Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

GENOME EDITING

Enhanced precision and efficiency

The expression of two DNA repair factors improves the recombination of single-stranded oligodeoxynucleotides with Cas9-induced double-strand breaks, facilitating precise and efficient gene editing.

Your institute does not have access to this article

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Fig. 1: Schematic of the DSB-repair pathways used in Cas9-induced genome editing.

References

  1. Barrangou, R. & Doudna, J. A. Nat. Biotechnol. 34, 933–941 (2016).

    CAS  Article  PubMed  Google Scholar 

  2. Daley, J. M. & Sung, P. Mol. Cell Biol. 34, 1380–1388 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  3. Heyer, W.-D., Ehmsen, K. T. & Liu, J. Annu. Rev. Genet. 44, 113–139 (2010).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. Chen, F. et al. Nat. Methods 8, 753–755 (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. Richardson, C. D., Ray, G. J., DeWitt, M. A., Curie, G. L. & Corn, J. E. Nat. Biotechnol. 34, 339–344 (2016).

    CAS  Article  PubMed  Google Scholar 

  6. Liang, X., Potter, J., Kumar, S., Ravinder, N. & Chesnut, J. D. J. Biotechnol. 241, 136–146 (2017).

    CAS  Article  PubMed  Google Scholar 

  7. Renaud, J.-B. et al. Cell Rep. 14, 2263–2272 (2016).

    CAS  Article  PubMed  Google Scholar 

  8. Paulsen, B. S. et al. Nat. Biomed. Eng. https://doi.org/10.1038/s41551-017-0145-2 (2017).

  9. Zimmermann, M. & de Lange, T. Trends Cell Biol. 24, 108–117 (2014).

    CAS  Article  PubMed  Google Scholar 

  10. Hanamshet, K., Mazina, O. M. & Mazin, A. V. Genes 7, 63 (2016).

  11. Maeder, M. L. & Gersbach, C. A. Mol. Ther. J. Am. Soc. Gene Ther. 24, 430–446 (2016).

    CAS  Article  Google Scholar 

  12. Dever, D. P. et al. Nature 539, 384–389 (2016).

    CAS  Article  PubMed  Google Scholar 

  13. Richardson, C. D. et al. Preprint at http://dx.doi.org/10.1101/136028 (2017).

  14. Ceccaldi, R., Sarangi, P. & D’Andrea, A. D. Nat. Rev. Mol. Cell Biol. 17, 337–349 (2016).

    CAS  Article  PubMed  Google Scholar 

  15. Ma, M. et al. Cell Res. 27, 578–581 (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ralf Kühn.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bashir, S., Kühn, R. Enhanced precision and efficiency. Nat Biomed Eng 1, 856–857 (2017). https://doi.org/10.1038/s41551-017-0159-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41551-017-0159-9

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing