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Gene delivery: Suddenly squeezed and shocked

A microfluidic device that integrates mechanical squeezing and electrical stimulation delivers DNA to the nucleus of cells at a rate of millions of cells per minute.

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Figure 1: Schematic of the delivery of large molecules, such as DNA, into the nucleus of cells through mechanical squeezing of the cell (which disrupts the plasma membrane), followed by electric-field disruption of the nuclear membrane.


  1. 1

    Stewart, M. P. et al. Nature 538, 183–192 (2016).

    CAS  Article  Google Scholar 

  2. 2

    Ding, X. et al. Nat. Biomed. Eng. 1, 0039 (2017).

  3. 3

    Sharei, A. et al. Proc. Natl Acad. Sci. USA 110, 2082–2087 (2013).

    CAS  Article  Google Scholar 

  4. 4

    Raab, M. et al. Science 352, 359–362 (2016).

    CAS  Article  Google Scholar 

  5. 5

    Denais, C. M. et al. Science 352, 353–358 (2016).

    CAS  Article  Google Scholar 

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Correspondence to Mehmet Toner.

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Toner, M. Gene delivery: Suddenly squeezed and shocked. Nat Biomed Eng 1, 0047 (2017).

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