Multiple origins of dichotomous and lateral branching during root evolution

Abstract

Roots of extant vascular plants proliferate through lateral branching (euphyllophytes) or dichotomy (lycophytes)1,2,3,4. The origin of these distinct modes of branching was key for plant evolution because they enabled the development of structurally and functionally different root systems that supported a diversity of shoot systems3,4,5,6. It has been unclear when lateral branching originated and how many times it evolved4,7,8. Here, we report that many euphyllophytes that were extant during the Devonian and Carboniferous periods developed dichotomous roots. Our data indicate that dichotomous root branching evolved in both lycophytes and euphyllophytes. Lateral roots then evolved at different times in three major lineages of extant euphyllophytes—the lignophytes, ferns and horsetails. The multiple origins of dichotomous and lateral root branching are extreme cases of convergent evolution that occurred during the Devonian and Carboniferous periods when the land-plant flora underwent a radiation in morphological diversity.

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Fig. 1: Differences between lateral and dichotomous root branching.
Fig. 2: Dichotomous root branching in L. goense.
Fig. 3: Multiple origins of dichotomous and lateral branching during root evolution.

Data availability

The paratype ULG 2057a and ULG 2057b of L. goense is housed in the collections of the University of Liège, Belgium. All other data supporting the findings of this study are included in the paper and its Supplementary Information.

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Acknowledgements

A.J.H. thanks C. Prestianni for help and hospitality while examining the collections of The University of Liège, as well as L. Ji and S. Snigirevsky for assistance with translation. A.J.H. was funded by the George Grosvenor Freeman Fellowship by Examination in Sciences, Magdalen College (Oxford); L.D. by a European Research Council Advanced Grant (EVO500, contract 250284), European Commission Framework 7 Initial Training Network (PLANTORIGINS, project identifier 238640) and European Research Council Grant (De NOVO-P, contract 787613); and C.M.B. by NERC grant NE/J007897/1.

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A.J.H. designed the project with advice from L.D. and C.M.B.; A.J.H. performed the analyses with assistance from C.M.B.; and A.J.H. and L.D. wrote the paper with comments from C.M.B.

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Correspondence to Alexander J. Hetherington.

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Hetherington, A.J., Berry, C.M. & Dolan, L. Multiple origins of dichotomous and lateral branching during root evolution. Nat. Plants 6, 454–459 (2020). https://doi.org/10.1038/s41477-020-0646-y

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