The frequency and orientation of cell division are regulated by intercellular signalling molecules; however, tissue-specific regulatory systems for cell divisions are only partially understood. Here, we report that the peptide hormone CLAVATA3/ESR-RELATED 9/10 (CLE9/10) regulates two different developmental processes, stomatal lineage development and xylem development, through two distinct receptor systems in Arabidopsis thaliana. We show that the receptor kinase HAESA-LIKE 1 (HSL1) is a CLE9/10 receptor that regulates stomatal lineage cell division, and BARELY NO MERISTEM (BAM) class receptor kinases are CLE9/10 receptors that regulate periclinal cell division of xylem precursor cells. Both HSL1 and BAM1 bind to CLE9/10, but only HSL1 recruits SOMATIC EMBRYOGENESIS RECEPTOR KINASES as co-receptors in the presence of CLE9/10, suggesting different signalling modes for these receptor systems.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Special thanks to H. Fukuda and Y. Kondo for providing us with all of the synthetic CLE peptides, Q.-J. Chen for the CRISPR vector (pHEE401E), K. Torii, D. Bergmann, S. Hou and the Arabidopsis Biological Resource Center seed stock centre for mutant seeds, and H. Deng and Y. Xu for mass spectrometry analysis. We thank K. Tsujimura for making vectors containing histone H2BsfGFP and mNEONGREEN optimized for A. thaliana. We thank Y. Matsubayashi and H. Shinohara for providing us with bam1-4 and bam1-4 clv1-101 mutants, discussions and preliminary experiments, and B. Morris and A. Deneve for advice on English usage. This work was supported by Grant-in-Aid for Scientific Research (B) number 25291060, Innovative Areas grant numbers 25113006 and 18H04837 to T.K. and the Ministry of Science and Technology of China (2015CB910200) to J.C.
The authors declare no competing interests.
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Qian, P., Song, W., Yokoo, T. et al. The CLE9/10 secretory peptide regulates stomatal and vascular development through distinct receptors. Nature Plants 4, 1071–1081 (2018). https://doi.org/10.1038/s41477-018-0317-4
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