Abstract
During infection of bladder epithelial cells, uropathogenic Escherichia coli (UPEC) can stop dividing and grow into highly filamentous forms. Here, we find that some filaments of E. coli UTI89 released from infected cells grow very rapidly and by more than 100 μm before initiating division, whereas others do not survive, suggesting that infection-related filamentation (IRF) is a stress response that promotes bacterial dispersal. IRF is accompanied by unstable, dynamic repositioning of FtsZ division rings. In contrast, DamX, which is associated with normal cell division and is also essential for IRF, is distributed uniformly around the cell envelope during filamentation. When filaments initiate division to regenerate rod cells, DamX condenses into stable rings prior to division. The DamX rings maintain consistent thickness during constriction and remain at the septum until after membrane fusion. Deletion of damX affects vegetative cell division in UTI89 (but not in the model E. coli K-12), and, during infection, blocks filamentation and reduces bacterial cell integrity. IRF therefore involves DamX distribution throughout the membrane and prevention of FtsZ ring stabilization, leading to cell division arrest. DamX then reassembles into stable division rings for filament division, promoting dispersal and survival during infection.
Similar content being viewed by others
Introduction
It is estimated that some 150 million people are affected by urinary tract infections (UTIs) each year1. The severity of these infections ranges from minor to life-threatening2,3,4. While there are many different species of bacteria that can cause UTIs, the major causative infectious agent is uropathogenic Escherichia coli (UPEC), which is responsible for more than 80% of reported cases5.
The UPEC infection cycle is initiated with bacteria being endocytosed by epithelial cells in the human urinary tract6. Once inside the host cells, the bacteria proliferate and undergo morphological changes to adopt cocci-like shapes while forming biofilm-like intracellular bacterial communities (IBC)7. These IBCs are exposed to various intercellular cues and develop into large communities that dominate the host cell. Eventually, the colony ruptures the host cell and disperses into the surrounding environment. During dispersal, a subset of the bacteria may stop dividing and grow into long filaments, some hundreds of microns long. The filaments can later divide back into rods and reinitiate the infection cycle8. Here we use the term infection-related filamentation (IRF) for this distinctive type of bacterial response.
It is not clear why UPEC undergoes IRF, but similar morphological changes are part of bacterial stress responses9,10,11. It has been suggested that filamentation might be a survival strategy to evade the immune system12. It is clear, however, that in order to reinitiate the infection cycle and colonize new host cells, the filaments must revert back to their typical rod shape13. As such, the reversion of filaments to rods is a crucial step in the UPEC infection cycle. Very little is known about how UPEC filaments reinitiate division to revert to rods.
During vegetative growth, binary fission is mediated by the bacterial cell division machinery—the divisome14. This multi-protein nanomachine is organized by the highly conserved FtsZ protein15. Together with a number of other early arriving proteins (e.g., the essential FtsA and ZipA16,17) FtsZ forms a proto-ring at the midcell when cells are primed to divide18. In a second step, the divisome matures by recruiting another set of core divisome proteins that have vital functions in chromosome segregation and peptidoglycan (cell wall) synthesis19. Apart from the core proteins, E. coli has specialized divisome-associated proteins that modify the septal peptidoglycan during division via a SPOR (Sporulation-related repeat) domain20,21.
SPOR domains are widely conserved and have been found in more than 1500 bacterial genomes22. E. coli, including UPEC, has four SPOR-containing proteins22, DamX, DedD, FtsN and RlpA, which localize to the division septum by binding to denuded glycan strands via their SPOR domains20,22. The molecular function(s) of the SPOR proteins in pathogenic bacteria are yet to be fully understood but important observations have been made during growth in standard laboratory conditions. FtsN, the only essential SPOR-domain protein, is thought to trigger division constriction23,24. RlpA is a lytic transglycosylase important for shape and division in Pseudomonas aeruginosa and Vibrio cholerae, but in E. coli the importance of this function is not clear25,26. DamX and DedD stimulate the enzymatic activity of cell wall synthases in E. coli27. Importantly, damX gene expression was found to be upregulated during the dispersal (filamentation) stage of bladder cell infection, and DamX is essential for UPEC IRF28.
In the present study, we have established an approach for studying UPEC filamentation and filament division using high-resolution and time-lapse fluorescence microscopy. We have used this approach to quantitatively characterize the growth and division dynamics of UPEC filaments dispersed from cultured bladder cells. To gain a better understanding of the dynamics and behaviour of the cell division machinery in IRF, we also visualized two divisome proteins expected to be important, FtsZ and DamX. Interestingly, FtsZ dynamically assembled in transient ring-like structures in filaments, which differs from normal rod cell division where one ring assembles and then completes division. On the other hand, DamX assembly into a ring always resulted in constriction of the membranes followed by division of the UPEC filaments. These data enable us to discriminate between two alternative models for the function and localization of DamX in UPEC filaments (post-infection). These models propose that DamX: (1) is localized at potential division sites along the filament during the arrest of cell division for filamentation, and then switches its function at those sites to promote division during reversal, or (2) is delocalized as part of the filamentation mechanism, and then assembles as rings, like in rod cell division, for the filament division process. The results consistently supported the second model.
Results
Shorter filaments are more likely to revert to rods
We used an in vitro urinary tract infection model system29, based on infection of cultured human bladder epithelial cells, to generate filaments of the model UPEC strain UTI8930. We transformed UTI89 with plasmid pGI529, which constitutively produced cytoplasmic sfGFP and did not alter growth rate, cell size or shape in standard laboratory conditions, i.e., during growth in rich medium at 37 °C (Supplementary Fig. 1). This strain was used interchangeably with wild-type (WT) UTI89 where appropriate. After infection of bladder epithelial cells with UTI89/pGI5, and then 20–22 h exposure to a flow of urine to induce filamentation and dispersal, a sample of the flow-through containing material from ruptured bladder cells was collected, washed once in PBS, resuspended in LB, immobilized on agarose pads, and imaged with light microscopy. As observed in previous studies, there was a mixture of both filaments and rods (Fig. 1a)13,29 and some areas of the slide showed noticeable clusters of filaments (Fig. 1b). We defined a cell as being a filament if it was at least two and a half times longer than the average length of cells grown in liquid LB culture. Using this definition, a filament was ~8 μm or more in length. The substantial heterogeneity in filament lengths (Fig. 1b, c) was consistent with the expected asynchronous dispersal of UPEC from the bladder cells29.
To characterize the growth and division dynamics of UPEC filaments, dispersal samples were placed on a LB-agarose pad and then time-lapse microscopy movies were captured with one image acquired every 3–5 min for at least 120 min. We observed several outcomes in the filaments. Relatively shorter filaments often grew into longer filaments (>20 μm) before starting to revert to rods, whereas some filaments elongated and then suddenly lysed (Supplementary Movie SM1). In contrast, many of the initially longer filaments (>50 μm) did not elongate further (Supplementary Movie SM2). Most of the shorter filaments (<50 μm) reverted to multiple rods over the course of imaging (Supplementary Movie SM3).
To quantify the state of filaments, we classified the fates of 343 filaments (from three different infection experiments) over at least 120 min into one of three groups: (1) ‘viable’, if they divided at least once, (2) ‘Lysed during imaging’, indicating filaments that did not divide and lysed during the image acquisition time (Supplementary Movie SM4), and (3) ‘dead’, representing shells of filaments that were highly translucent and did not divide nor extend in length at all during the imaging period. Supplementary Fig. 2 shows examples of ‘viable’ and ‘dead’ filaments. Average lengths of filaments at the start of imaging and their classification frequencies were: viable = 56.3 ± 56.5 μm (n = 208, ~61%), growing but no division = 117.6 ± 73.4 μm (n = 67, ~20%) and dead = 122.6 ± 79 μm (n = 68, ~20%) (Fig. 1c). Therefore, most of the viable filaments were significantly shorter than the average, indicating that extensive elongation carries a significant risk to filament survival.
To further investigate filament viability, we used a LIVE/DEAD differential staining assay (Supplementary Fig. 3) in which live cells are distinguished by their uptake of the SYTO9 green dye whereas only dead cells take up the Propidium Iodide (PI) red dye (Fig. 1d, e, PI shown in magenta). We examined 275 UTI89 filaments from 3 independent infections and found that 36% stained dead (Fig. 1f), similar to the proportion of non-dividing and dead filaments classified above (~39%). We were also able to follow filaments dying over time, transitioning from green to magenta (Fig. 1g and Supplementary Movie SM5). From here on, we only characterized filaments that were classified as viable.
Filaments divide with increasing frequency during reversion to rods
When further analysing the time-lapse movies of the UTI89 filaments, we noticed large differences in the time it took for filaments to initiate division and revert to rods, i.e., the time from placing filaments on the agarose pads until the first division event. This varied from just a few minutes to more than 2 h in the same sample, suggesting a large heterogeneity in metabolic states between filaments. Moreover, filaments elongated by <1 μm, to >140 μm before the first division (mean (±SD) = 19.78 ± 22.3 μm, n = 101, Fig. 2a, b). The time-averaged elongation rate (∆L/∆t) varied from 0.08 μm min−1 to 1.76 μm min−1 in some filaments, with an overall mean of 0.55 ± 0.4 μm min−1 (Fig. 2c). For comparison, UTI89 showed an elongation rate of 0.1 μm min−1 in standard LB culture (Supplementary Fig. 5), which is similar to the well-studied K-12 strain. We then estimated the specific growth rate (i.e., elongation rate normalized by length, Fig. 2d) to see if the elongation rate correlates with the starting length of the filaments, as would be expected for homogeneous growth of the filament population. However, we observed poor correlation (Fig. 2e), consistent with a large heterogeneity of metabolic states between filaments (Fig. 2a, d). There was no apparent correlation between the length of a filament at the start of imaging and the time to first division (Fig. 2f).
We next monitored individual filament division over four consecutive generations, after which most cells became too crowded and moved out of focus (Supplementary Movie SM6). Most filaments grew initially (Fig. 2b) and then often divided towards one end, resulting in rod cells ‘pinching-off’ (Fig. 2g and Supplementary Movie SM6). The sequential generations of the ‘mother’ filament occurred at a progressively greater frequency (Fig. 2h), often appearing at multiple locations near both ends. This was also be seen by measuring the mean filament interdivision time (Fig. 2j), where, for example, there was a ~25 % difference between filament division frequencies after the second and the fourth cells had pinched off (second cell, \({\bar{X}}_{2{nd}}=20.83\) min, compared to the fourth cell, \({\bar{X}}_{4{th}}=15.47\) min). The short interdivision times would suggest that multiple potential division sites may be primed as soon as—or even before—the previous generation has pinched off from the mother filament. This is similar to division frequency during recovery from antibiotic-induced filamentation31.
The size of newborn cells pinching off from the filament was quite consistent over time, with mean lengths of 4.66 ± 2.32 μm for the first cell, and then 4.89 ± 1.92 μm, 5.05 ± 2.59 μm, and 5.08 ± 3.1 μm for subsequent generations. There was a noticeable size skew, however, seen at all generations, with ~80% of newborns shorter than average and ~10% of newborns at least twice the average length (Fig. 2k and Supplementary Movie SM6). We then tracked the size of each pinched-off cell for 3–4 of its subsequent divisions (see Fig. 2l), which revealed a slight decrease in mean length over four generations: 4.83 ± 2.33 μm (n = 129), 4.01 ± 1.71 μm (n = 203), 3.44 ± 0.67 μm (n = 397) and 3.11 ± 0.5 μm (n = 664), respectively (Fig. 2m). For comparison, cells that had not been through an infection cycle were on average 2.79 ± 0.35 μm (n = 340) in length at birth (Fig. 2m, ‘No inf.’). The first and second divisions sometimes gave rise to abnormally long cells (Fig. 2m), but this corrected over the next one or two generations, with essentially all pairs of daughter cells in the fourth generation being the same length at birth (Fig. 2n).
Nucleoid distribution during filament reversal
To see how DNA partitioning was maintained during reversal, we also looked at the localization of chromosomes in filaments of UTI89 transformed with a plasmid expressing HupA-RFP32 (which did not interfere with UTI89 growth; Supplementary Fig. 1). Fluorescence microscopy revealed that the DNA was distributed along the lengths of filaments and in somewhat irregular patches (Supplementary Fig. 4a, 0 min). Filaments contained on average 28.2 nucleoids per 100 μm cell length, corresponding to ~3.5 μm cell length per nucleoid, which is similar to normal rods. The average length of nucleoid masses in filaments without visible constrictions was 2.05 ± 0.86 μm (n = 305), with a primarily bimodal size distribution (Supplementary Fig. 4b, red). In post-divisional rods that had been pinched off from a filament, and were about to divide, the average nucleoid length was 1.3 ± 0.24 μm (n = 254) (Supplementary Fig. 4b, green), similar to non-infection UTI89 growing in LB of 1.45 ± 0.32 μm (n = 308) (Supplementary Fig. 4b, blue). There was an increasing degree of symmetry in chromosome partitioning over generations (Supplementary Fig. 4b, Inset), consistent with the observed progression of cell size symmetry (Fig. 2k).
Dynamic repositioning of FtsZ-rings in IRF
Our results above showed that filaments surviving the infections rapidly coordinate reversion to rods when conditions become favourable for vegetative growth. It is not known when the cell division machinery assembles in UPEC filaments to reinitiate division. To determine whether or not the divisome was at least partially pre-assembled in filaments, we transformed the UTI89 strain with a plasmid producing FtsZ-mCitrine33 as a divisome marker, since all other essential division proteins are dependent on FtsZ assembly at midcell during vegetative growth14,34. Fluorescence microscopy of UPEC filaments expressing FtsZ-mCitrine released from the bladder cells after an infection showed that all filaments, regardless of length, had at least one FtsZ-ring assembled, but often more. Interestingly, however, movies revealed that FtsZ-rings formed, disassociated, and re-formed multiple times in various locations within the same filament (Fig. 3a, c and Supplementary Movies SM7 and SM8), and did not always end in a division event. There were on average less FtsZ rings per unit length in filaments compared to rods (i.e., one ring per ~4 μm) (Fig. 3d). These results indicate that FtsZ rings are significantly less stable in filaments than in rods. The dynamic repositioning of FtsZ rings suggests that in IRF the divisome is regulated at the stage of Z-ring stabilization or maturation.
The presence of FtsZ may help to explain how the first newborn daughter cells from filaments were able to quickly divide again (~15 min, Fig. 2h). Consistent with our results for wild-type UTI89, when the first divisions took place in filaments labelled with FtsZ-mCitrine, the majority (~75%) were away from midcell and predominantly towards the poles (Fig. 3a, b, yellow arrows, and Supplementary Movie SM7). Some FtsZ-rings were also inherited by daughter cells after division had occurred elsewhere (Fig. 3e, left column, yellow arrows).
Knockout of damX affects vegetative cell division and IRF in UTI89
Since FtsZ rings did not always result in a division even, it is likely that other division regulators are involved. We turned our attention to DamX, as it was previously found to be essential for IRF in UTI8928, though how it regulates IRF is unknown.
Previous studies on DamX showed it is localized to the divisome during vegetative growth in a strictly FtsZ-dependent manner35, but its exact role in division has been uncertain since damX deletion causes no obvious division defect in E. coli K-1222,23 (Fig. 4a, b). We found that UTI89∆damX grew at rates indistinguishable from WT in midlog LB cultures. However, the cells were on average ~33% longer than WT (UTI89∆damX = 5.25 ± 3.38 μm (n = 228), and WT UTI89 = 3.94 ± 1.14 (n = 171)) (Fig. 4c, d and Supplementary Fig. 5). Most noticeably, UTI89∆damX showed a skewed distribution of cell size compared to WT; ~15% of UTI89∆damX cells were 8 μm or longer, which is at least twice the average WT UTI89 length (Fig. 4d, Supplementary Movie SM9, and Supplementary Fig. 5). UTI89∆damX cells producing mEos3.2-DamX from a plasmid (pMP6) showed a mean cell length of 4.87 ± 1.41 μm (n = 219) and a substantial rescue of the elongated population, with ~3% cells longer than 8 μm, compared to more than 15% for the uncomplemented UTI89∆damX, and around 1% for WT UTI89 (Fig. 4e, f and Supplementary Fig. 5). Western blots indicated that DamX production levels were similar in E. coli K-12 (BW25113), UTI89 and UTI89∆damX + pMP6 (Supplementary Fig. 5d). Thus, loss of DamX results in a moderate vegetative division defect in UTI89, which is not seen in the model K-12 strain.
We then produced FtsZ-mCitrine to a level that does not interfere with growth or division36 (Supplementary Fig. 5e) in UTI89∆damX. In midlog LB cultures, FtsZ-mCitrine localized to midcell in the normal-sized rods, followed by constriction and division, like in the WT. However, in the elongated cells, the FtsZ rings frequently formed and then disassembled without division (Fig. 4g, white arrows, Supplementary Movie SM10), reminiscent of FtsZ-mCitrine in IRF (Fig. 3). DamX thus appears to influence FtsZ ring stability in UTI89 filaments.
In the infection model, we observed that UTI89∆damX failed to undergo IRF, consistent with the previous study28 (Fig. 4h). Instead, we saw a moderate level of elongation in a subpopulation of cells, as seen in the LB cultures. Interestingly, we also saw a relatively high proportion (~10%) of short cells that appeared translucent indicating a loss of cell integrity (Fig. 4h). We speculate that cells may be attempting to filament but were unable due to the deletion of damX. Use of the UTI89∆damX/pMP6 strain largely restored IRF and cell integrity, indicating that the mEos3.2-DamX protein fusion is mostly functional and confirms that DamX is responsible for the observed phenotypes (Fig. 4j and Supplementary Movie SM11). The mean length was 13.17 ± 15.72 μm (n = 184), with more than ~50% of the cells 8 μm or longer (Fig. 4k, Supplementary Fig. 5). DamX production from the plasmid was ~50% higher than WT (Fig. 4I) and did not interfere with growth or division of filaments (Supplementary Movie SM11). We conclude that DamX influences filamentation and cell integrity during infection.
A switch in DamX localization is associated with the onset of filament division
To investigate how DamX acts in IRF and filament division, we visualized mEos3.2-DamX produced from an anhydrotetracycline-inducible expression plasmid (pDD7) in UTI89. [Note, for unknown reasons mEos3.2 fluorescence was too weak in the constitutive expression plasmid pMP6.] In UTI89/pDD7, mEos3.2-DamX fusion represented ~60% of the total cellular DamX (Supplementary Fig. 1c), which did not affect vegetative growth or division (Supplementary Fig. 1a, b), consistent with previous studies36,37,38. High-level DamX overexpression has previously been shown to cause filamentation39,40, and its strong upregulation is associated with the UTI89 filamentous response during infection28. We found that the moderate production of mEos3.2-DamX in UTI89 did not inhibit filament reversal (Figs. 4 and 5 and Supplementary Figs. 1c and 5). Furthermore, in bacteria released from infections, mEos3.2-DamX localization and dynamics in the rod-cell population were similar to what has been seen in K-12 during vegetative growth22 (Supplementary Movie SM12).
To determine the localization and subcellular structures of mEos3.2-DamX in UPEC filaments, we imaged UTI89/pDD7 obtained from infections using live-cell single-molecule PhotoActivated Localization Microscopy (PALM). Filaments at various stages of preparation for division were identified in these samples. Those that appeared to be at an early stage of preparing to divide were identified by the appearance of broad mEos3.2-DamX rings prior to visible invagination (Fig. 5a, b). Consistent with this, measurement of the diameter of the broad mEos3.2-DamX rings and of the filament 1 μm either side showed a ratio of 1:1.05–0.95 (Fig. 5c, d). The fluorescence intensity profiles along the filament axis showed that the pre-constriction mEos3.2-DamX localizations were as broad as >500 nm (Fig. 5c, e). Active division was identified by a significantly narrower diameter of the mEos3.2-DamX ring than the filament (Fig. 5f, g). The mean axial breadth of these condensed mEos3.2-DamX rings along the cell length was 116.5 ± 13.4 nm (n = 122) (Fig. 5h), similar to midlog LB samples (102.5 ± 20.2 nm, n = 150) (Supplementary Fig. 6). In accordance with previous observations of another SPOR-domain protein, FtsN41, mEos3.2-DamX seemed to linger at the division sites after the inner membranes had closed, before being completely disassembled (Fig. 5j–l). Interestingly, live pre-divisional filaments were also identified, in which mEos3.2-DamX showed dispersed cell envelope localization with no clear rings (Fig. 5m). This suggested that filament formation may not require ring localization of DamX.
Given that DamX is essential for IRF, we sought to capture its behaviour during the switch from filament elongation through to completion of division and several subsequent generations. Time-lapse epifluorescence imaging of UTI89/pDD7 filaments collected from infection and recovering on LB agar pads revealed mEos3.2-DamX assembled as rings at different places along the length of filaments (Supplementary Movie SM13). Figure 6a shows the formation of at least three generations of mEos3.2-DamX division rings in one filament over time, and importantly, that the filaments then reverted into rods at those sites. In contrast to what we observed for the FtsZ-mCitrine rings, once an mEos3.2-DamX ring formed, it remained in place until division was completed.
Measurements of mEos3.2-DamX ring position indicated that there was a preference for rings to form initially at the approximate ¼, ½ or ¾ positions along the filament length regardless of the length, which was predominantly in the range of ~8–40 μm (n = 134) (Fig. 6b). The data also indicated that cells pinching off in the second and third generation did so closer to the poles (Fig. 6b, c). Note that the mother filament would have grown in length between each division.
In line with our observations that the apparent time between division of daughter cells from filaments decreased with successive generations, we wanted to know if this was also consistent in the first division (previously, we could only determine the relative time from the second division without a division marker, i.e., Fig. 2). That is, would the first division ring be present longer than the second before a division? To do this, we followed mEos3.2-DamX ring formation, constriction, and subsequent division in filaments over time. Indeed, the average time from the first mEos3.2-DamX ring formation to the first division was 56 ± 13 min (SD), and there was a significant reduction in time to the next (second) division: 15 ± 10 min (SD) (Fig. 6d, e). This was also in good agreement with our observation for the FtsZ-mCitrine rings that completed constriction (Fig. 3e).
We also wanted to see if the intensity of mEos3.2-DamX varied during ring formation, as this would be proportional to the total cellular expression level. The total cellular fluorescence did not change significantly with the formation of new mEos3.2-DamX rings (Fig. 6f); compared to filaments with no visible rings, the relative mean cellular fluorescence with one ring was 1.02 ± 0.07 (n = 45), while relative mean cellular fluorescence with two rings was 1.03 ± 0.15 (n = 32) (Fig. 6f, inset). Curiously, we also noticed that the mEos3.2-DamX rings did not always divide in the order that they initially formed (Fig. 6g). It was observed that around 20% of filaments had the second generation of rings pinch off prior to the first (Fig. 6h).
Since mEos3.2-DamX was assembled at multiple sites along filaments, we wondered whether septal PG (sPG42) synthesis/hydrolysis also occurred at multiple sites at the same time. To test this, we pulse-labelled filaments during reversal to detect regions of sPG synthesis/hydrolysis with an OregonGreen488-labelled Fluorescent D-amino acid (OGDA)43,44. As we saw with mEos3.2-DamX, OGDA was observed in multiple fluorescent rings (Fig. 7a), indicating that active peptidoglycan synthesis was present at multiple sites and that multiple cell division machineries were active in multiple rings simultaneously. We also observed various levels of OGDA intensity, indicating differing sPG activity in the rings. The localization of OGDA at many division sites preceded visible constriction, suggesting that the cell division machineries would be assembled at that stage, whereas other OGDA localizations were clearly associated with later-stage division constrictions in both filaments and rods (Fig. 7b, c).
Discussion
We have used live-cell time-lapse, epifluorescence and super-resolution microscopy to quantitatively characterize the growth and division of filamentous Uropathogenic E. coli (UPEC) released from the dispersal stage of infection of bladder epithelial cells29,30. UPEC filaments can grow to hundreds of microns long in UTI, but we found that shorter filaments (<50 μm) were more likely to survive and revert to rods in culture. UPEC filamentation has been proposed as a survival strategy to prevent their potential phagocytosis by immune cells or improve surface adhesion10,11,13. Our results suggest that UPEC filamentation is a response to stress during dispersal and exposure to human urine—and the stress is not always overcome.
Prior to filament reversal (i.e., division into rods) most filaments undertook a period of variable growth (on LB agarose), where elongation rates ranged from 0.08 μm min−1 to 1.72 μm min−1, or up to ~20 times the elongation rate of a standard rod cell. Since filamentation combines the growth power of multiple rods and effectively links the strength of their cell walls together in one cell body, we propose that the rapid directional elongation of filaments physically aids UPEC dispersal from the IBC biofilm and host cell carcass, helping them to ‘push out’ into the extracellular environment. Indeed, the extrusion-like images of UPEC filaments exiting host cells are consistent with this potential selective advantage29,45.
The elongation rate of UPEC filaments was not correlated with the initial length of the filament, indicating a substantial variability in metabolic states of filaments regardless of length. Consistent with this, the time to the first division ranged from just a few minutes to more than two hours. The substantial heterogeneity may be related to both the asynchronous dispersal from the bladder cells and the different morphological fates of individual IBC dispersal events11,13,29.
Most UPEC filaments divided near the ends, releasing a smaller ‘pinched-off’ cell. Over the first few divisions, the filament interdivision time became shorter, much like filament reversal after antibiotic treatment31. Furthermore, the pinched-off UPEC cells became shorter over the initial generations too, approaching the size of standard rods. The combined results suggest that division tends towards balanced growth over a few generations. Previous studies looking at the cell division protein FtsA during reversal of E. coli filaments induced by antibiotics and DNA damage (SOS response) found that division rings assemble evenly along the cell, sometimes only transiently and then at new locations, with one division event at a time occurring at the time31. In the current study, multiple overlapping divisions were common during reversal of IRF, predominantly towards the poles. Despite the strain and condition differences between the studies, the findings suggest that significantly different mechanisms of filamentation and reversal underpin the antibiotic, SOS, and IRF responses, highlighting the importance of understanding these differences at both strain and protein function levels for future development of infection therapeutics.
We investigated the role of two distinct cell division proteins FtsZ and DamX in UTI89 filaments. FtsZ has long been regarded as the main initiator of the division and assembly of its associated machinery in most bacteria. DamX, a member of the SPOR-domain family of proteins, has a less well-defined role but is known to localize to midcell in an FtsZ-dependent manner during vegetative growth. In cephalexin-induced filaments, FtsZ rings predominantly form at constant intervals46. To test if this was the case also in IRF and take a step towards understanding the mechanism of division arrest in IRF, we visualized how fluorescently tagged FtsZ behaved in UTI filaments. In contrast to what was observed with cephalexin, the Z-rings in UTI filaments were formed and spaced in highly irregular intervals. On the other hand, similar to what was seen for the antibiotic-induced filaments31, Z-rings were unstable in IRF and showed dynamic repositioning prior to division. This suggests that FtsZ ring instability is a part of the division arrest mechanism leading to filamentation, and that the eventual re-initiation of division in IRF is regulated by another protein that controls the behaviour of FtsZ during the switch from filament elongation to division. Once a FtsZ ring started to constrict, it remained at the division site until a late stage when it disassembled similar to what has been observed in rods37.
A recent study found that DamX—previously implicated in sPG remodelling during vegetative growth—was upregulated during the dispersal stage of infection when filamentation occurs and is also essential for UPEC filamentation28. This led to the hypothesis that DamX switches functions to block division at potential division sites during UPEC filamentation. Surprisingly, we instead observed that fluorescently-tagged DamX (mEos3.2-DamX) was initially not assembled at potential division sites in UPEC filaments, but was dissipated around the cell envelope and assembled later as rings where filaments eventually divided. In contrast to what we observed for FtsZ, once a DamX ring was formed it remained formed, a division event always followed, and the DamX rings were stable until the completion of division. Our single-molecule data suggest that mEos3.2-DamX assembles into rather broad rings at the quarter or half positions along filaments, consistent with the expected locations of division, and then condenses leading up to constriction, where mEos3.2-DamX rings showed very similar dimensions as those formed by related cell division components during E. coli division36,47,48. Lastly, mEos3.2-DamX remained assembled at the division septum until after the inner membranes were visibly separated. The lingering of the protein at the division site suggests a role for DamX in sPG regulation until building of the new cell wall is completed.
In summary, the current data suggest that during filamentation, DamX has a role in blocking division that involves dissipated localization around the whole membrane, possibly associated with sequestration of division proteins or the direct inhibition of division throughout the length of the filament. DamX then switches function for reversal to assist in cell division in a manner that structurally resembles normal cell division. DamX also has an apparently more significant role in vegetative division of UTI89 than in the traditional model strain, K-12. DamX has also been implicated in morphology changes and virulence in other pathogens (e.g., Vibrio parahaemolyticus), indicating a broader overall function of the protein across species49. Further studies on diverse strains will tell if one is atypical, but the difference may also reflect the apparent importance of DamX to the UPEC infection cycle. We envision that the IRF response and the infection model may become an increasingly important tool for the study of morphology, division and survival of pathogenic bacteria.
Methods
Ethics approval
This study has human research ethics approval from the UTS Human Research Ethics Committee (HRCH REF No. 2014000452). All urine donors gave their approval to participate in this study by informed consent under this study’s ethics approval number above.
Bacterial cell growth
A single colony of respective E. coli UTI89 strain was grown overnight in a 20 ml LB (Difco #244620) culture at 37 °C without shaking, to favour the expression of type-1 pili, which facilitates adhesion to the bladder cells during infection5. Antibiotics were added when needed (ampicillin 100 μg ml−1 (Sigma #A9518), spectinomycin 25 μg ml−1 (Sigma #S4014)). The following morning, the cultures were pelleted and resuspended in 1× PBS (Bio-Rad #1610780) to a concentration of OD600 0.2 and added to the infection model. Cells not used in the infection model were diluted 1:50 and grown to an OD600 of ~0.4 before imaging to measure cell length and fluorescence profiles. Bacterial strains and plasmids used in this study are listed in Table 1.
Plasmid construction
The expression plasmid pAJM.011 was used as a backbone in the construction of pDD7 (mEos3.2-DamX). This plasmid was part of the Marionette sensor collection50, which was obtained from Addgene (Kit #1000000137). Initially, the aph coding sequence in pAJM.011 was replaced by the bla coding sequence. In a second step, the coding sequence for EYFP was replaced by the coding sequences for mEos3.2 and FtsN. Finally, the coding sequence for FtsN was replaced by that for DamX. All fragments were amplified by polymerase chain reaction (PCR) using Q5 DNA polymerase (New England Biolabs, USA, NEB #M0491). Fragments to be ligated contained regions of 20-30 bp of homology, and were cloned using the in vivo DNA assembly method51. The coding sequence for bla was obtained from pET15b, mEos3.2 from Genscript (The Netherlands) and FtsN/DamX from the E. coli strain MG1655. Oligonucleotide synthesis and DNA sequencing was performed by Eurofins Genomics (Germany).
pGI429 was used as a plasmid backbone for the construction of a plasmid constituently producing mEos3.2-DamX. Firstly, pGI4 was digested with NcoI and BamHI-HF (New England Biolabs, #R0193S and # R31136R, respectively). mEos3.2-DamX was amplified from pDD7 using Q5 DNA polymerase (New England Biolabs) with oligonucleotides synthesized by Integrated DNA Technologies (USA). The products of the previous reactions were cloned using Gibson52 assembly and used to transform DH5α. The resulting plasmid, denoted pMP6, was purified and sequenced by AGRF (Australia). All primers used in this study are available in the Supplementary Information file.
Fluorescent protein production
WT UTI89 strain was transformed with pGI529, pMP6 or pDD7 to produce strains expressing sfGFP or mEos3.2-DamX, respectively. sfGFP (from pGI5) and mEos3.2-DamX (from pMP6) did not need inducer as it was produced from a constitutive promoter29. mEos3.2-DamX (pDD7) expression was induced by adding 1 μM anhydrotetracycline (aTc) for 1 h to the infection cultures prior to collecting filaments.
FtsZ-mCitrine (pHC054)33 was induced by adding 5 μM isopropyl β-D-1-thiogalactopyranoside IPTG for 1 h to the infection cultures prior to collecting filaments. We confirmed that the expression level of the FtsZ-mCitrine construct was not interfering with growth and division in neither rods nor filaments (Fig. 3e and Supplementary Fig. 1). Previous studies have shown that production of fluorescent protein fusions to FtsZ (e.g., FtsZ-mEos3 and FtsZ-GFP) below ~ 70% of total cellular FtsZ does not measurably alter cellular growth, divisions nor Z-ring morphology36. FtsZ-mCitrine in the UPEC filaments was produced to less than 50% of total cellular FtsZ (Fig. 3f).
WT UTI89 strain was transformed with pSTC01132 to produce a strain expressing HupA-RFP. HupA-RFP was induced for 1 h by adding 20 μM IPTG to the cultures containing filaments.
Preparation of human urine samples
Urine from two different donors (one female and one male) was collected in the morning and stored for at least 2 days at 4 °C. Samples were centrifuged at 4500 × g for 8 min and the supernatant was filtered through a 0.2 μm membrane filter. The specific gravity was determined by comparison to pure water using a gravity meter. Only urine samples in the pH range 5.12-5.83 and USG range 1.024–1.031 g ml−1 were used29. Filter sterilized samples were frozen at −20 °C until required. Note, to be as consistent as possible, only one batch of urine was used per technical replicates, since both pH and USG can alter the proportion of filamentation in the samples13.
Urinary tract infection model
We based our infection model on a previously described approach13,29, with minor modifications. In summary, on day 1, flow chambers (IBIDI μ-Slides I0.2 Luer, Cat#: 80166) were seeded with PD07i53 epithelial bladder cells at a concentration of ~3 × 106 cells in EpiLife Medium (Gibco, #MEPI500CA) supplemented with growth supplements and antibiotics (HKGS, #S0015, and 100 μg ml−1 Pen/Strep, SIGMA #P4333). The channels were left overnight for cells to adhere and grow into a confluent layer. The next day, flow channels were connected to New Era pumps via tubing and 20 ml disposable syringes, and a flow (15 μl min−1) of fresh EpiLife (supplemented with HKGS) without antibiotics was maintained for 18–20 h. On day three, to induce infection, bladder cells were exposed to bacterial cultures at a concentration of OD600 0.2 for 20 min at a flow rate of 15 μl min−1. Following this step, the media was changed back to EpiLife (supplemented with HKGS), after an initial flow of 100 μl min−1 to flush out the excess bacteria, and flowed for 9 h to allow bacteria to adhere to and invade the epithelial bladder cells. This step was followed by flow (15 μl min−1) of EpiLife (supplemented with HKGS) in the presence of 100 μg/ml gentamycin for 20 h to allow for the formation of intracellular bacterial communities (IBCs), as well as to kill and wash away any lingering extracellular bacteria. Following this 20 h, the media was changed to human urine (with pH between 5.12 and 5.83 and Urine Specific Gravity of at least 1.024 g ml−129) with flow (15 μl min−1) for at least 20 h to induce filamentation and dispersal of the bacteria from the bladder cells. Since the proportion of live filaments is expected to vary with different batches and sources of urine13; to avoid variability we used urine from the same batch in experimental replicates. Filaments were collected from the back-opening of the flow channels and resuspended in LB for fluorescent protein expression, Live/Dead cell staining and direct imaging, or stained (FDAA labelling) and fixed.
Live/Dead cell staining
Staining was performed as recommended by the manufacturer (LIVE/DEAD BacLight Bacterial Viability Kit, #L7012, Molecular Probes). Briefly, 3 μl of each dye mixture (SYTO9 and Propidium iodide) was added for each mL sample culture and mixed thoroughly. The dye/sample mixture was incubated for 15 min in the dark at room temperature. After incubation, 2–3 μl culture was spotted directly onto agarose pads (1.5% w/v in LB) for imaging. We also confirmed that cells and filaments divided and reverted using only SYTO9 labelling (Supplementary Fig. 3).
FDAA labelling
WT UTI89 filaments were labelled with 1 mM OGDA43, using a protocol similar to what has been described before54, for 5 min and washed twice in PBS, before fixation in 70% ice-cool ethanol for 1 h. After ethanol fixation, cells were washed twice in PBS and placed on agarose pads (1.5% w/v in water) for imaging.
Microscopy and imaging
Samples were placed on pre-made agarose (1–1.5% w/w) pads in LB (for dynamics) or M9 (single-molecule imaging) media in 65 μl gene frames (Thermo Scientific, AB0577), left to immobilize and imaged directly thereafter.
Live cell epifluorescence and bright-field time-lapse imaging of filaments reverting back to rods was done using a Nikon Ti2-E deconvolution microscope with extra-large Field Of View optics (full FOW of the microscope was 25 mm, this ensured us that long filaments could always be imaged within one FOW), equipped with a CFI Plan Apo Lambda DM ×100 oil objective (NA 1.45), and an environmental chamber set at 37 °C (Okolab cage incubator). Images were acquired every 2–10 min as required. Excitation of fluorophores was performed using a Lumencore Spectra II module, and fluorescence was detected using a back-illuminated Andor Sona 4.2 sCMOS camera. GFP and mEos3.2 (in the green state) emission was collected through a FITC filter and RFP emission through a Cy5 filter. Filters were from Semrock.
Live cell PALM imaging was performed on a Nikon (Ti2-E) N-STROM v5 with NIS v.5.30 using a 100x 1.49 NA oil objective in TIRF mode. Cover glass slides were washed with 95% EtOH, air dried, cleaned for at least 3 min with a plasma cleaner (Harrick plasma, PDC-23G) and used within 15 min of cleaning. Imaging was consistently performed at room temperature (~23 °C). Drift correction during image acquisition was minimized using the integrated PFS4 (Perfect Focus System). 100 nm multi-colour TetraSpeck beads were used as fiducial markers. Prior to single-molecule acquisition, the green state of mEos3.2 was excited by a 488 nm laser to acquire an epifluorescence image using a FITC emission filter cube. For single-molecule acquisition, mEos3.2 was photoconverted to its red state continuously by a 405 nm laser with increasing working powers ranging between 0.1 and 5 W cm−2. As readout, mEos3.2 was excited by a 561 nm laser line operating at an average power between 1 and 2 kW cm−2. The emission was collected by a quad band (Quad405/488/561/647 filter dual cSTORM). The exposure time was 20 ms and 4000–5000 images were typically acquired for each set of images. Images were captured using a sCMOS Flash 4.0 v3 (Hamamatsu) camera.
Note: we also imaged UTI89 cells fixed with 2% paraformaldehyde using standard protocols55, but we noticed that the fixative seemed to interfere with protein localization. This loss of localization was not noticed when imaging live cells, similar to what previous studies with other cell division proteins have found56,57.
Image analysis
Epi-fluorescence images and movies were visualized and analysed in Fiji58. Images and movies were post-processed for background subtraction (rolling ball radius 36 pixels). PALM images were processed using the Nikon N-STORM software and Fiji plugin ThunderSTORM59 using Gaussian blur of 20 nm for visualization.
Cell dimensions of rods and filaments during reversal
Cell lengths for rods that had not been through an infection were extracted from bright-field images of respective strains. Filaments were followed for one to four divisions on agarose pads using bright field imaging. To simplify the analysis, we only followed cells pinching off from one side of a filament. ‘Birth’ was determined from the first image frame where the poles of cells were not connected. ‘Lengths at birth’ and ‘symmetry at birth’ statistics were generated from length measurements in MicrobeJ60 or from manually traced lengths in Fiji. In bright field, a filament was manually classified as dead when it was clearly translucent and had similar grey values as the background. Fluorescence profiles were generated in Fiji and Full Width at Half Maxima (FWHM) was extracted by fitting a Gaussian to the generated curves. Plots and statistics were generated using the web apps PlotsOfData61 and SuperPlotsOfData62, as well as Origin9 pro (Origin Lab, US). Figures were prepared using Adobe Illustrator.
Western blotting
A volume of cells corresponding to OD600 0.1 was collected from cell cultures. The samples were suspended in loading buffer and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins were transferred to nitrocellulose membranes using a semi-dry Transfer-Blot apparatus (Bio-Rad). The membranes were blocked in 5% (w/v) milk and probed with anti-sera to DamX38 (1:5000) or FtsZ (1:3333) (Agrisera).
Statistical analysis
All statistical analysis were performed in either Origin Pro 9 or GraphPad Prism 9. P values are from unpaired two-tailed t tests, and are scored as P > 0.05 = not statistically significant, *P < 0.05, **P < 0.005 and ***P < 0.0005 if nothing else is indicated in the text.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The data generated in this study are provided either in the Supplementary Information or in the Source Data file. Source data are provided with this paper.
References
Stamm, W. E. & Norrby, S. R. Urinary tract infections: disease panorama and challenges. J. Infect. Dis. 183(Suppl 1), S1–4 (2001).
Wagenlehner, F. M., Tandogdu, Z. & Bjerklund Johansen, T. E. An update on classification and management of urosepsis. Curr. Opin. Urol. 27, 133–137 (2017).
Tandogdu, Z., Cai, T., Koves, B., Wagenlehner, F. & Bjerklund-Johansen, T. E. Urinary tract infections in immunocompromised patients with diabetes, chronic kidney disease, and kidney transplant. Eur. Urol. Focus 2, 394–399 (2016).
Hooton, T. M. Clinical practice. Uncomplicated urinary tract infection. N. Engl. J. Med. 366, 1028–1037 (2012).
Flores-Mireles, A. L., Walker, J. N., Caparon, M. & Hultgren, S. J. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat. Rev. Microbiol. 13, 269–284 (2015).
Sihra, N., Goodman, A., Zakri, R., Sahai, A. & Malde, S. Nonantibiotic prevention and management of recurrent urinary tract infection. Nat. Rev. Urol. 15, 750–776 (2018).
Anderson, G. G. et al. Intracellular bacterial biofilm-like pods in urinary tract infections. Science 301, 105–107 (2003).
Hannan, T. J., Mysorekar, I. U., Hung, C. S., Isaacson-Schmid, M. L. & Hultgren, S. J. Early severe inflammatory responses to uropathogenic E. coli predispose to chronic and recurrent urinary tract infection. PLoS Pathog. 6, e1001042 (2010).
Miller, C. et al. SOS response induction by beta-lactams and bacterial defense against antibiotic lethality. Science 305, 1629–1631 (2004).
Justice, S. S., Hunstad, D. A., Cegelski, L. & Hultgren, S. J. Morphological plasticity as a bacterial survival strategy. Nat. Rev. Microbiol. 6, 162–168 (2008).
Justice, S. S., Hunstad, D. A., Seed, P. C. & Hultgren, S. J. Filamentation by Escherichia coli subverts innate defenses during urinary tract infection. Proc. Natl Acad. Sci. USA 103, 19884–19889 (2006).
Justice, S. S. et al. Differentiation and developmental pathways of uropathogenic Escherichia coli in urinary tract pathogenesis. Proc. Natl Acad. Sci. USA 101, 1333–1338 (2004).
Andersen, T. E. et al. Escherichia coli uropathogenesis in vitro: invasion, cellular escape, and secondary infection analyzed in a human bladder cell infection model. Infect. Immun. 80, 1858–1867 (2012).
McQuillen, R. & Xiao, J. Insights into the structure, function, and dynamics of the bacterial cytokinetic FtsZ-ring. Annu. Rev. Biophys. 49, 309–341 (2020).
Bi, E. F. & Lutkenhaus, J. FtsZ ring structure associated with division in Escherichia coli. Nature 354, 161–164 (1991).
Hale, C. A. & de Boer, P. A. Recruitment of ZipA to the septal ring of Escherichia coli is dependent on FtsZ and independent of FtsA. J. Bacteriol. 181, 167–176 (1999).
Addinall, S. G. & Lutkenhaus, J. FtsA is localized to the septum in an FtsZ-dependent manner. J. Bacteriol. 178, 7167–7172 (1996).
Hale, C. A. & de Boer, P. A. Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli. Cell 88, 175–185 (1997).
Haeusser, D. P. & Margolin, W. Splitsville: structural and functional insights into the dynamic bacterial Z ring. Nat. Rev. Microbiol. 14, 305–319 (2016).
Yahashiri, A., Jorgenson, M. A. & Weiss, D. S. Bacterial SPOR domains are recruited to septal peptidoglycan by binding to glycan strands that lack stem peptides. Proc. Natl Acad. Sci. USA 112, 11347–11352 (2015).
Ursinus, A. et al. Murein (peptidoglycan) binding property of the essential cell division protein FtsN from Escherichia coli. J. Bacteriol. 186, 6728–6737 (2004).
Arends, S. J. et al. Discovery and characterization of three new Escherichia coli septal ring proteins that contain a SPOR domain: DamX, DedD, and RlpA. J. Bacteriol. 192, 242–255 (2010).
Gerding, M. A. et al. Self-enhanced accumulation of FtsN at division sites and roles for other proteins with a SPOR domain (DamX, DedD, and RlpA) in Escherichia coli cell constriction. J. Bacteriol. 191, 7383–7401 (2009).
Pichoff, S., Du, S. & Lutkenhaus, J. The bypass of ZipA by overexpression of FtsN requires a previously unknown conserved FtsN motif essential for FtsA-FtsN interaction supporting a model in which FtsA monomers recruit late cell division proteins to the Z ring. Mol. Microbiol. 95, 971–987 (2015).
Jorgenson, M. A., Chen, Y., Yahashiri, A., Popham, D. L. & Weiss, D. S. The bacterial septal ring protein RlpA is a lytic transglycosylase that contributes to rod shape and daughter cell separation in Pseudomonas aeruginosa. Mol. Microbiol. 93, 113–128 (2014).
Weaver, A. I. et al. Lytic transglycosylases RlpA and MltC assist in Vibrio cholerae daughter cell separation. Mol. Microbiol. 112, 1100–1115 (2019).
Pazos, M. et al. SPOR proteins are required for functionality of class a penicillin-binding proteins in Escherichia coli. mBio https://doi.org/10.1128/mBio.02796-20 (2020).
Khandige, S. et al. DamX controls reversible cell morphology switching in uropathogenic Escherichia coli. mBio https://doi.org/10.1128/mBio.00642-16 (2016).
Iosifidis, G. & Duggin, I. G. Distinct morphological fates of uropathogenic Escherichia coli intracellular bacterial communities: dependency on urine composition and pH. Infect Immun. https://doi.org/10.1128/IAI.00884-19 (2020).
Chen, S. L. et al. Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: a comparative genomics approach. Proc. Natl Acad. Sci. USA 103, 5977–5982 (2006).
Wehrens, M. et al. Size laws and division ring dynamics in filamentous Escherichia coli cells. Curr. Biol. 28, e975 (2018).
Wegner, A. S., Alexeeva, S., Odijk, T. & Woldringh, C. L. Characterization of Escherichia coli nucleoids released by osmotic shock. J. Struct. Biol. 178, 260–269 (2012).
Söderström, B., Badrutdinov, A., Chan, H. & Skoglund, U. Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells. Nat. Commun. 9, 4323 (2018).
Du, S. & Lutkenhaus, J. At the heart of bacterial cytokinesis: the Z ring. Trends Microbiol. 27, 781–791 (2019).
Arends, S. J. R. et al. Discovery and characterization of three new Escherichia coli septal ring proteins that contain a SPOR domain: DamX, DedD, and RlpA. J. Bacteriol. 192, 242–255 (2010).
Coltharp, C., Buss, J., Plumer, T. M. & Xiao, J. Defining the rate-limiting processes of bacterial cytokinesis. Proc. Natl Acad. Sci. USA 113, E1044–1053 (2016).
Söderström, B. et al. Disassembly of the divisome in Escherichia coli: evidence that FtsZ dissociates before compartmentalization. Mol. Microbiol. 92, 1–9 (2014).
Williams, K. B. et al. Nuclear magnetic resonance solution structure of the peptidoglycan-binding SPOR domain from Escherichia coli DamX: insights into septal localization. Biochemistry 52, 627–639 (2013).
Lyngstadaas, A., Lobner-Olesen, A. & Boye, E. Characterization of three genes in the dam-containing operon of Escherichia coli. Mol. Gen. Genet. 247, 546–554 (1995).
Tenorio, E. et al. Systematic characterization of Escherichia coli genes/ORFs affecting biofilm formation. FEMS Microbiol. Lett. 225, 107–114 (2003).
Söderström, B. et al. Coordinated disassembly of the divisome complex in Escherichia coli. Mol. Microbiol. 101, 425–438 (2016).
McCausland, J. W. et al. Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism. Nat. Commun. 12, 609 (2021).
Söderström, B., Ruda, A., Widmalm, G. & Daley, D. O. An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy. Microbiology 166, 1129–1135 (2020).
Kuru, E. et al. In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew. Chem. 51, 12519–12523 (2012).
Mulvey, M. A. et al. Induction and evasion of host defenses by type 1-piliated uropathogenic Escherichia coli. Science 282, 1494–1497 (1998).
Pogliano, J., Pogliano, K., Weiss, D. S., Losick, R. & Beckwith, J. Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential division sites. Proc. Natl Acad. Sci. USA 94, 559–564 (1997).
Söderström, B., Chan, H., Shilling, P. J., Skoglund, U. & Daley, D. O. Spatial separation of FtsZ and FtsN during cell division. Mol. Microbiol. 107, 387–401 (2018).
Lyu, Z. et al. FtsN activates septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthase in E. coli. Preprint at bioRxiv https://doi.org/10.1101/2021.08.23.457437 (2021).
Gode-Potratz, C. J., Kustusch, R. J., Breheny, P. J., Weiss, D. S. & McCarter, L. L. Surface sensing in Vibrio parahaemolyticus triggers a programme of gene expression that promotes colonization and virulence. Mol. Microbiol. 79, 240–263 (2011).
Meyer, A. J., Segall-Shapiro, T. H., Glassey, E., Zhang, J. & Voigt, C. A. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat. Chem. Biol. 15, 196–204 (2019).
Watson, J. F. & Garcia-Nafria, J. In vivo DNA assembly using common laboratory bacteria: a re-emerging tool to simplify molecular cloning. J. Biol. Chem. 294, 15271–15281 (2019).
Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343–U341 (2009).
Klumpp, D. J. et al. Uropathogenic Escherichia coli potentiates type 1 pilus-induced apoptosis by suppressing NF-kappaB. Infect. Immun. 69, 6689–6695 (2001).
Hsu, Y. P. et al. Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem. Sci. 8, 6313–6321 (2017).
Fu, G. et al. In vivo structure of the E. coli FtsZ-ring revealed by photoactivated localization microscopy (PALM). PLoS One 5, e12682 (2010).
Buss, J. et al. In vivo organization of the FtsZ-ring by ZapA and ZapB revealed by quantitative super-resolution microscopy. Mol. Microbiol. 89, 1099–1120 (2013).
Buss, J. et al. A multi-layered protein network stabilizes the Escherichia coli FtsZ-ring and modulates constriction dynamics. PLoS Genet. 11, e1005128 (2015).
Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676–682 (2012).
Ovesny, M., Krizek, P., Borkovec, J., Svindrych, Z. & Hagen, G. M. ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging. Bioinformatics 30, 2389–2390 (2014).
Ducret, A., Quardokus, E. M. & Brun, Y. V. MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis. Nat. Microbiol. 1, 16077 (2016).
Postma, M. & Goedhart, J. PlotsOfData-A web app for visualizing data together with their summaries. PLoS Biol. 17, e3000202 (2019).
Goedhart, J. SuperPlotsOfData-a web app for the transparent display and quantitative comparison of continuous data from different conditions. Mol. Biol. Cell 32, 470–474 (2021).
Acknowledgements
The authors would like to thank David Weiss (University of Iowa) for sharing the DamX antisera and the BW25113∆damX strain. Jakob Møller-Jensen is acknowledged for kindly sharing the UTI89∆damX strain. The authors are grateful to Dr. Tamika Blair for initial help with the infection model. The authors acknowledge support and equipment from the UTS Microbial Imaging Facility (MIF) and a UTS infrastructure grant for a Nikon N-STORM single-molecule microscope. B.S. was supported by a CPDRF scheme from the University of Technology Sydney, and I.G.D. was supported by an Australian Research Council Future Fellowship (FT1601000010).
Author information
Authors and Affiliations
Contributions
B.S. and I.G.D. conceptualized the study. D.O.D. and M.P. contributed reagents and advice on experiments. B.S. and M.J.P. carried out all experiments. B.S. led the data analysis with input from I.G.D. B.S. and I.G.D. wrote the paper with input from D.O.D. and M.J.P.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Peer review
Peer review information
Nature Communications thanks Matthew Jorgenson and the other anonymous reviewer(s) for their contribution to the peer review of this work. Peer review reports are available.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Source data
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Söderström, B., Pittorino, M.J., Daley, D.O. et al. Assembly dynamics of FtsZ and DamX during infection-related filamentation and division in uropathogenic E. coli. Nat Commun 13, 3648 (2022). https://doi.org/10.1038/s41467-022-31378-1
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41467-022-31378-1
This article is cited by
-
Harnwegsinfekte: Blick in die Zukunft der Therapie
MMW - Fortschritte der Medizin (2023)
-
Optimising expression of the large dynamic range FRET pair mNeonGreen and superfolder mTurquoise2ox for use in the Escherichia coli cytoplasm
Scientific Reports (2022)
-
Filamentous morphology of bacterial pathogens: regulatory factors and control strategies
Applied Microbiology and Biotechnology (2022)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.