# Neonatal genetics of gene expression reveal potential origins of autoimmune and allergic disease risk

## Abstract

Chronic immune-mediated diseases of adulthood often originate in early childhood. To investigate genetic associations between neonatal immunity and disease, we map expression quantitative trait loci (eQTLs) in resting myeloid cells and CD4+ T cells from cord blood samples, as well as in response to lipopolysaccharide (LPS) or phytohemagglutinin (PHA) stimulation, respectively. Cis-eQTLs are largely specific to cell type or stimulation, and 31% and 52% of genes with cis-eQTLs have response eQTLs (reQTLs) in myeloid cells and T cells, respectively. We identified cis regulatory factors acting as mediators of trans effects. There is extensive colocalisation between condition-specific neonatal cis-eQTLs and variants associated with immune-mediated diseases, in particular CTSH had widespread colocalisation across diseases. Mendelian randomisation shows causal neonatal gene expression effects on disease risk for BTN3A2, HLA-C and others. Our study elucidates the genetics of gene expression in neonatal immune cells, and aetiological origins of autoimmune and allergic diseases.

## Introduction

Infancy is a critical period during which physiological and developmental changes impact the pathogenesis of conditions later in life1,2. Many complex diseases, in particular immune and respiratory conditions, are partially determined by genetic predisposition and early-life environment exposures, such as microbes or allergens3,4,5. Yet, despite increasing evidence of its importance, little is known about the early-life genetic regulation of gene expression, nor its relevance to predisposition for diseases in adulthood.

Expression quantitative trait loci (eQTL) studies have provided insights into the gene regulatory effects of genetic variants and their relationship with complex disease6,7. The majority of eQTLs have been identified in adult tissues, while eQTLs in perinatal tissues have only been explored recently: for example, eQTLs identified in foetal placentas8 and foetal brains9 are enriched for genetic variants associated with growth (e.g. adult height) and neuropsychiatric (e.g. schizophrenia) traits, respectively. In addition to genetic variation, disease development is influenced by individual and cell-type-specific responses to external stimuli. Understanding the interaction between eQTLs and these stimuli can give insights into condition(s), whether they be cell type, microbe or temperature, under which genetic variants may influence disease. Previous studies have investigated response eQTLs (reQTLs), eQTLs with genetic effects modified by external stimulation, in CD14+ monocytes10,11, macrophages12, dendritic cells13,14, and CD4+ T cells15. However, reQTL studies to date have been largely performed using adult samples, and we currently have limited knowledge of how neonatal immune gene expression is regulated in response to stimuli.

Here, we characterise the genetics of gene expression in the innate and adaptive arms of the neonatal immune system using purified cord blood samples from 152 neonates16,17,18. In these samples, we identify cis- and trans-eQTLs of myeloid cells and CD4+ T cells, as well as reQTLs for myeloid cells stimulated with lipopolysaccharide (LPS; a component of bacterial cell walls) and CD4+ T cells stimulated with phytohemagglutinin (PHA; a pan-T cell mitogen). By comparing our neonatal cis-eQTLs with previously identified adult eQTLs, we find eQTLs that are specific to neonates. Using mediation analysis, instances of putative trans gene regulation are investigated to identify cis regulatory mechanisms. We show evidence for the shared genetic basis of neonatal eQTLs and reQTLs with common autoimmune and allergic diseases, and many of such colocalisations are cell type- or stimulation-specific. Finally, we use Mendelian randomisation to uncover the causal effects of neonatal gene expression on risk of immune-mediated diseases. Ultimately, we highlight the potential importance of the perinatal period in understanding the origins of immune-mediated disease.

## Results

### Genetics of neonatal gene expression in innate and adaptive immunity

We performed eQTL analysis on cell preparations derived from in vitro cultures of resting and stimulated neonatal immune cells from 152 neonates of the Childhood Asthma Study (CAS) cohort (Fig. 1), which were enriched respectively for non-adherent T cells and adherent myeloid cells as detailed in ‘Methods’, with the latter largely being monocytes and macrophages. Cell purities could not be experimentally confirmed by flow cytometry due to limited blood volumes that could be collected from neonates; rather, in silico analyses were utilised to estimate the abundances of relevant cell types using CIBERSORTx19. These analyses indicated dominance of the relevant T-cell and myeloid signatures (Supplementary Fig. 1). Among 135 genotyped individuals, 106 and 119 had gene expression data passing QC for both resting and stimulated conditions of myeloid and T-cell cultures, respectively, and 95 individuals had post-QC data for all four cultures. The total number of samples available for eQTL analysis was 116 for resting myeloid cells, 125 for LPS-stimulated myeloid cells, 126 for resting T cells, and 127 for PHA-stimulated T cells.

To identify cis-eQTLs, we applied a hierarchical procedure to correct for multiple testing within each experimental condition at 5% false discovery rate (FDR; ‘Methods’). Stimulated cells yielded a larger number of cis-eQTLs and associated genes (eGenes) than resting cells (1347 vs. 971 eGenes in PHA-stimulated vs. resting T cells, respectively; 376 vs. 136 in LPS-stimulated vs. resting myeloid cells, respectively; Fig. 2a, Supplementary Data 1). To investigate the differences in numbers of eGenes between conditions, we repeated the analysis controlling for differences in sample size (randomly sampling 116 samples in each condition). This yielded similar results to the numerical distribution of cis-eGenes: 1231, 900, and 350 in PHA-stimulated T cells, resting T cells, and LPS-stimulated myeloid cells, respectively. The lower number of eQTLs in myeloid cells may be explained by fewer genes being expressed (Supplementary Fig. 2).

For eGenes with eQTLs in multiple experimental conditions, we performed conditional analysis to distinguish whether these were independent or shared signals between conditions (‘Methods’). The majority (74%) of eQTL signals were specific to one cell type or stimulatory condition (Supplementary Fig. 3A), consistent with previous observations10. About 10–50% of the condition-specific signals were replicated using a multivariate adaptive shrinkage (mash) model (Supplementary Fig. 3B)20. We observed a majority of cis-eQTL effects after stimulation: 60% (262 of 376) of eGenes in LPS-stimulated myeloid cells and 58% (778 of 1347) in PHA-stimulated T cells. Using a two-step conditional analysis (‘Methods’), PHA-stimulated T cells had the largest number of eGenes (6.3%; Supplementary Table 1) with multiple independent eQTL signals. GARFIELD enrichment analysis21 showed that the cis-eSNPs were enriched in 3′ untranslated regions (UTR), 5′ UTR, and exon regions (Supplementary Fig. 4), consistent with known mechanisms of cis-eQTLs.

We compared our resting and LPS-stimulated myeloid cells with those from adults in Kim-Hellmuth et al.11 using the mash model20. We found that 6.7% and 7.2% of the cis-eQTLs tested in both studies (105 and 332 eQTLs) were specific to neonates at LFSR of 0.05 (Fig. 2b). Similarly, we compared resting neonatal T cells to adult T cells of the BLUEPRINT project22: after excluding 332 cis-eQTLs that were not tested in the adult T cells, we found that 24.7% neonatal cis-eQTLs were not active in adult T cells. We have also used the Storey and Tibshirani q-value approach23 to investigate eQTL sharing between neonatal and adult tissues. Replication rate quantified by $$\pi$$1 statistic was 0.998 for resting myeloid cells, 0.987 and 0.947 for LPS-stimulated myeloid cells comparing to monocytes treated by LPS for 90 min and 6 h, respectively, in Kim-Hellmuth et al., and 0.879 for resting T cells. We note, however, that this approach may overestimate the replication rate as it does not take the direction or magnitude of eQTL effects into account.

### Genetics of neonatal gene expression in response to stimuli

To quantify how genetic regulation of gene expression is altered by external stimuli, we identified response eQTLs (reQTLs) and response eGenes (reGenes) by performing interaction tests on the top eSNPs of each eGene in myeloid cells and T cells separately, and controlling FDR at 5% using permutation-determined P-values (‘Methods’). In myeloid cells, we identified 125 significant reQTLs involving 125 unique reGenes (31% of 398 myeloid eGenes); in T cells, we identified 956 reQTLs involving 918 unique reGenes (52% of 1749 T cell eGenes), among which 38 reGenes had distinct cis-eQTLs in two conditions where both eQTLs were reQTLs (Supplementary Data 2). Consistent with our findings for cis eQTLs and eGenes, the number of reQTLs and proportion of reGenes were greater in stimulated compared to resting conditions.

For two reQTLs, the direction of eQTL effect changed between conditions (Fig. 2c). The C allele of the top eSNP (rs5751775) for DDT (D-dopachrome tautomerase), a gene functionally related to the inflammatory cytokine MIF (migration inhibitory factor), increased DDT transcription in resting T cells but decreased expression after PHA stimulation (Supplementary Fig. 5A, Supplementary Data 2). Similarly, the T allele of the top eSNP (rs13068288) for ZNF589 increased transcription of ZNF589 in resting T cells but reduced expression after PHA stimulation (Supplementary Fig. 5B, Supplementary Data 2).

### Disentangling trans and cis effects using mediation analysis

While we were relatively underpowered to detect trans-eQTLs, we maintained stringent significance thresholds and compared overlapping cis and trans effects to generate hypotheses of gene regulation (‘Methods’). We identified 25 trans-eQTLs in T cells (10 in resting, 15 in PHA-stimulated), and one trans-eQTL in myeloid cells (Fig. 3a, Supplementary Data 3) at a genome-wide FDR of 5%. Notably, the trans-eQTL for MYH10, a component of myosin heavy chain which regulates cytokinesis, was shared across all four experimental conditions; furthermore, the same eQTL was associated with multiple trans-eGenes in T cells: MIR130A and STX1B in resting and stimulated T cells, and IP6K2 and MIR1471 in resting T cells only (Fig. 3a).

Consistent with previous reports that trans-eQTLs are enriched for cis-eQTLs24, we found that multiple trans-eQTLs (the single trans-eQTL in myeloid cells, 6 of the 10 in resting T cells, and 3 of the 15 in PHA-stimulated T cells) were significantly associated with local genes in cis (Fig. 3a, Supplementary Data 3). The trans-eQTL for MYH10 was also a cis-eQTL for RPS26 (part of the 40S subunit of the ribosome) in all conditions except PHA-stimulated T cells. The top eSNP (rs1131017) for RPS26, located in its 5’ UTR, was also a cis-eQTL for SUOX (sulphite oxidase, a homodimer in the intermembrane space of mitochondria) in resting and stimulated T cells. Separately, the trans-eQTL (rs687492) for microRNA MIR330 was also a cis-eQTL for the long non-coding RNA SNHG8 in resting and stimulated T cells.

Mediation analysis revealed that the trans-eQTL effects of rs687492 on MIR330 were cis mediated through SNHG8 (Fig. 3b, c, Supplementary Table 2; ‘Methods’), indicating a potential pathway containing this lncRNA-miRNA cross-talk. Furthermore, mediation analysis also revealed the regulatory logic of cis-eQTL (rs1131017) for SUOX and RPS26, identifying that its trans-effects on MYH10, MIR130A, and IP6K2 were mediated through RPS26 and not SUOX in resting T cells (Fig. 3d, Supplementary Table 2).

### Genetic overlap with immune-mediated diseases

To investigate the genetic overlap between neonatal gene expression and disease, we used a multi-pronged approach. First, we performed GARFIELD enrichment analyses to test for significant overlaps between the cis-eQTLs and variants associated with immune-mediated diseases in genome-wide association studies (GWAS; ‘Methods’). We found widespread enrichment amongst cis-eQTLs for genetic variants associated with diseases such as allergic disease (asthma, hay fever, or eczema) and inflammatory bowel disease. Conversely, same analysis with non-immune-related traits such as educational attainment identified limited enrichment (Supplementary Fig. 6). Specifically, we did not observe significant enrichment of GWAS signals for educational attainment in myeloid eQTLs; there was evidence of enrichment in resting and PHA-stimulated T cells but enrichment was not as strong (effect sizes 0.43 and 0.52 respectively) compared to immune-mediated diseases (ranging from 1.13 to 7.37).

Second, we performed colocalisation analysis25 to identify variants sharing regulatory (eQTL) and disease-associated (GWAS) signals (‘Methods’). In total, we observed 68 colocalisations, involving 5, 9, 15, and 17 independent cis-eQTLs in resting myeloid cells, LPS-stimulated myeloid cells, resting T cells, and PHA-stimulated T cells, respectively (Fig. 4a, Supplementary Data 4). Our analysis replicated the colocalisation of the cis-eQTL for BACH2 in resting T cells with variants for type 1 diabetes22, and also revealed widespread colocalisation with autoimmune thyroid disease, celiac disease, multiple sclerosis, rheumatoid arthritis (Supplementary Fig. 7). BACH2 encodes a transcriptional repressor that restrains terminal differentiation and promotes the development of memory lymphocytes including CD8+ T cells26 and B cells27. At the BACH2 locus, the A allele at the top eSNP (rs72928038) was associated with decreased BACH2 expression and increased risk of the above diseases. This was consistent with previous studies which showed that mutations and loss-of-function variants of BACH2 resulted in immunodeficiency and disruption to regulatory T cell function, with subsequent autoimmunity28,29.

We found 17 colocalisations of reQTLs and disease variants, in total involving 12 reQTLs: one myeloid reQTL specific to LPS stimulation (eQTL for CTSH), and 11 reQTLs in T cells, among which eight were specific to PHA stimulation. Notably, the reQTL for IL13 in PHA-stimulated T cells colocalised with GWAS hits associated with asthma, allergic sensitisation, and allergic disease (Fig. 4, Supplementary Data 4). The T allele of the top eSNP (rs1295686) was associated with greater IL13 expression in PHA-stimulated T cells as well as increased risk of all three diseases (Fig. 4b). rs1295686 is intronic to IL13 and in strong LD (r2 > 0.98) with four other eSNPs, including a Gln144Arg missense SNP (rs20541) in IL13. At the CCL20 locus, the A allele of the cis-eQTL/reQTL (rs13034664) in PHA-stimulated T cells was associated with lower CCL20 expression as well as increased risk of childhood-onset asthma (Fig. 4a, Supplementary Fig. 8, Supplementary Data 4). CCL20 is part of the CCR6-CCL20 receptor-ligand axis, a key driver of dendritic cell chemotaxis30.

Our analyses uncovered complex condition-specific colocalisations at multiple loci. The ubiquitin ligand UBASH3A, known to regulate apoptosis in T cells, had two independent cis-eQTLs in resting and stimulated T cells which were also reQTLs. However, only the cis-eQTL in resting T cells (rs1893592) colocalised with celiac disease, rheumatoid arthritis, and primary sclerosing cholangitis (PSC; Fig. 4, Supplementary Data 4). Cis-eQTLs for CTSH, which encodes the lysosomal cysteine proteinase cathepsin H, colocalised with signals for different diseases in a cell type- and condition-specific manner (Supplementary Fig. 9, Supplementary Data 4). Cis-eQTLs for CTSH in resting myeloid cells and resting T cells both colocalised with GWAS hits for celiac disease, narcolepsy, and type 1 diabetes, which has been observed in immune cell types from adults: colocalisation with causal variants of celiac disease and narcolepsy was reported in macrophages12, and type 1 diabetes in adult monocytes22. On the other hand, cis-eQTLs for CTSH in LPS-stimulated myeloid cells and PHA-stimulated T cells both colocalised with primary biliary cirrhosis (PBC).

### Causal effects of condition-specific gene expression on immune-mediated diseases

To identify putative causal effects of neonatal gene expression on risk of autoimmune and allergic disease, we performed two-sample Mendelian randomisation (MR) analysis using cis-eQTLs as genetic instruments, the neonatal cis-eGene as exposure, and disease as outcome (‘Methods’). We tested the 52 eGenes which had three or more genetic instruments available, and the diseases above, for which we had GWAS summary statistics available. We considered genes for which at least three of four MR methods (inverse variance weighted, weighted median, weighted mode, and MR Egger) were in agreement in detecting significant causal effects (P-value ≤ 0.05) on a disease without significant pleiotropic effects (Supplementary Data 5).

In our MR analysis, we found multiple conditions where neonatal gene expression had a causal effect on multiple diseases (Fig. 5), including BTN3A2 (butyrophilin subfamily 3 member A2), HLA-C (major histocompatibility complex class I molecule), MICB (ligand for an activatory receptor expressed on natural killer cells, CD8+ αβ T cells, and γδ T cells), ZNRD1 (RNA polymerase 1 subunit), and SLC22A5 (carnitine transporter) (Supplementary Data 5). BTN3A2 had a relatively large number of genetic instruments for resting (seven to eight) and stimulated (three to five) T cells, and the causal estimates were similar between these two conditions (Supplementary Figs. 10, 11). In resting T cells, increased expression of BTN3A2 was causally associated with decreased risk of asthma (weighted mode causal estimate = −0.056 log odds decrease per s.d. increase in BTN3A2), both childhood- and adult-onset asthma (−0.047 and −0.039, respectively), allergic rhinitis (−0.044), PSC (−0.440), and systemic lupus erythematosus (SLE; −0.256). Conversely, increased BTN3A2 expression was associated with increased risk of inflammatory bowel disease (IBD; 0.025), including Crohn’s disease (0.053), as well as risk of PBC (0.129), where PBC variants also showed colocalisation with BTN3A2 eQTLs (Fig. 4, Supplementary Fig. 12). Expression of HLA-C in T cells showed strong causal association with autoimmunity, in particular positive causal effects on psoriasis, SLE, PSC, multiple sclerosis, IBD, and ulcerative colitis; and negative causal effects on juvenile idiopathic arthritis, PBC, and rheumatoid arthritis (Fig. 5, Supplementary Figs. 13, 14).

## Discussion

In this study, we investigated the genetic regulation of gene expression in cells of neonatal innate and adaptive immunity, and its relationship to the genetic basis of autoimmune and allergic diseases. In this context, we illustrated that the genetics of gene expression in neonates is strongly specific to cell type and stimulatory condition, and is distinct from that of adults. We described regulatory mechanisms of eQTLs whose putative trans effects had evidence of mediation via gene expression in cis. In exploring the potential early-life origins of disease, our analyses showed an extensive genetic overlap of genetic variants associated with immune-mediated diseases and those with effects on gene expression in neonatal immune cells. Finally, Mendelian randomisation showed that myriad changes in gene expression at birth had potentially causal effects on autoimmune and allergic disease risk.

We observed stimuli changing the direction of eQTL effects in resting and PHA-stimulated T cells. This was the case for the C allele of reQTL (rs5751775) and DDT, a cytokine structurally and functionally related to MIF, a critical regulator of both innate and the adaptive immune response31. It is known that eQTL effects can change direction in immune cells32. In the Genotype-Tissue Expression (GTEx) Project6, the direction of eQTL effect for the C allele at rs5751775 was also variable across liver, pancreas, stomach, testis, brain, and muscle tissues, though the variable direction of eQTL effect may also be due to LD contamination, i.e. when the top eSNPs tag two distinct eQTLs in two conditions.

Our results suggest a trans mediation role for RPS26, rather than SUOX, consistent with previous studies linking RPS26 to IP6K233,34. RPS26 and SUOX were both identified as significant mediators in multiple tissues in the GTEx dataset; however, within the same tissue, the trans-associations mediated through SUOX were not identical to those through RPS26, indicating distinct effects of SUOX and RPS26 on distant genes34. RPS26 encodes a ribosomal subunit protein which, apart from its role in ribosome assembly and translation35, is involved in various other cellular processes, including nonsense-mediated mRNA decay36 and p53 transcriptional activity37. It is likely that the broad trans effects of RPS26 may be related to its ribosomal functions.

At the CCL20 locus, the reQTL (rs13034664) in PHA-stimulated T cells colocalised with childhood-onset asthma (Fig. 4a, Supplementary Fig. 8). CCL20 encodes a C–C chemokine ligand that binds to a G protein-coupled receptor, and elevated CCL20 expression has been shown in airways of patients with chronic obstructive pulmonary disease (COPD)38 and asthma39. CCL20 induces mucin production by binding to its unique receptor (CCR6) in human airway epithelial cells40. However, in PHA-stimulated T cells, the A allele of the reQTL (rs13034664), which was linked to increased risk of childhood-onset asthma, was associated with reduced CCL20 expression (Supplementary Fig. 8B). This reQTL and its direction of effect were replicated by others in activated CD4+ T cells7. T cells themselves respond to CCL20 via binding to CCR6, and this process is observed during allergen provocation41. It is possible that the pathophysiological mechanism is not T cell production of CCL20, but rather their response to it – hence the reduced CCL20 expression in a possible feedback loop. Altogether, these findings reinforce the cell-type-specific nature of eQTL and reQTL associations.

On the other hand, the reQTL for IL13 in neonatal PHA-stimulated T cells appeared to share causal variants with allergic disease and asthma, suggesting that this reQTL may affect allergic disease risk via mechanisms that do involve T cell activation and interleukin 13 (IL-13). PHA is a pan-T cell mitogen, and downstream intracellular signalling may be shared between PHA and allergen-mediated T cell activation. IL-13 is produced by activated CD4+ and CD8+ T cells42 among others, promoting immunoglobulin E (IgE) production in B cells43. IL-13 has been shown to induce asthma symptoms including airway hyper-responsiveness, increased total serum IgE, and increased mucus production in murine models44. Increased IL-13 expression is observed in sputum and bronchial biopsy in mild45 and severe46 asthma and can serve as a biomarker for severe refractory asthma47. Therapies that target IL-13 (anti-IL-13 antibodies) have been developed, such as lebrikizumab and tralokinumab however, they show inconsistent or only modest effects in treating severe asthma exacerbations in phase 3 clinical trials. Our results are consistent with IL-13 as a therapeutic target for asthma and suggest the potential for improved efficacy if treatment is targeted towards specific genetic subgroups.

We found strong causal effects of neonatal BTN3A2 expression on various autoimmune and allergic diseases. Butyrophilin (BTN) family members are immunoglobulin-like molecules that act as immune check-point regulators with roles in self-tolerance48. Increased BTN3A2 protein expression is a favourable prognostic biomarker in epithelial ovarian cancer patients, and indicates a higher density of intraepithelial infiltration of T cells49. BTN3 family members BTN3A1 and BTN3A3 are proximal to BTN3A2. The antigen-presenting BTN3A1 is critical to human γδ T cell activation50. A recent study showed that BTN3A2 regulated subcellular localisation of BTN3A1, and both were required for T cell activation51. Previous MR analysis found BTN3A2 lung expression had a causal effect on COPD risk52. Our findings suggest that altered neonatal BTN3A2 expression, with presumed subsequent dysfunction in immunomodulation, plays a role in the pathogenesis of multiple inflammatory conditions.

In our study, neonatal HLA-C expression in both myeloid cells and T cells was causally associated with multiple autoimmune diseases such as psoriasis, SLE, and primary biliary cirrhosis. HLA-C encodes an MHC Class I receptor which presents antigens to CD8+ T cells. It is also the major ligand for killer immunoglobulin-like receptors (KIRs), which regulate the activity of natural killer (NK) cells. HLA-C is an established locus for psoriasis susceptibility53 and the interaction between HLA-C and ERAP1 is associated with psoriasis risk, where ERAP1 variants only have psoriasis effects in individuals with the HLA-C risk allele54. In our analysis of resting neonatal T cells, the largest causal effect of HLA-C expression was for psoriasis.

Our study had limitations. Cord blood may be under the influence of in utero exposures (e.g. smoking, drug exposures, maternal stress) which may confound associations with maternal, placental or foetal genetics55, and may affect neonatal gene expression through epigenetic mechanisms56. In utero exposures, epigenetics, or maternal genetics were not measured in this cohort. While maternal cells are present in cord blood samples, the vast majority of cord blood cells are from neonates; previous estimates of the proportion of maternal cells in cord blood are of the order of 10−4 to 10−5 of nucleated foetal blood cells57. While the adherence protocol used in this study can lead to some level of cell activation, this is also the case with other cell isolation methods such as MACS, and there is no a priori reason to suspect that this would deleteriously influence subsequent responsiveness. Since this is a study in neonates, the blood volumes collected were necessarily small and mandated the use of micro methods for in vivo studies where all available material was needed to generate transcriptional profiles, and ancillary assays, e.g. quantification of cell purity, were not able to be performed. While RNA-seq technology allows the identification of additional signals, such as isoform QTLs, which are relevant in neonatal tissues, it can be prone to artefacts when constructing large-scale libraries with low input RNA; therefore, as this neonatal study necessarily relied on low sample volumes, we consciously chose gene expression arrays due to their robustness in this setting and well-established normalisation approaches. In silico analyses to infer cell type abundances showed good cell purities (Supplementary Fig. 1), and the estimated abundances were correlated with the PEER factors included when mapping eQTLs; thus, while differences in cell composition may not be completely controlled, we believe these effects are minimal. Furthermore, we are still relatively power-limited with regard to trans-eQTL analyses and further studies with greater sample sizes, particularly in the neonatal and early childhood settings, are necessary.

In conclusion, our study shows the remarkable complexity of the genetic regulation of gene expression in the innate and adaptive arms of the immune system at birth, and its potential role in the pathogenesis of autoimmunity and allergic disease.

## Methods

### Study cohort and RNA sample preparation

The study population is a subset of the CAS, a prospective birth cohort of 234 individuals followed from birth to up to 10 years of age16,17,18. This project was approved by the University of Western Australia Human Ethics Committee. Cord blood samples were collected for 152 individuals at birth, and we have obtained informed consent from all participants. One million peripheral blood mononuclear cells (PBMCs) from each individual were stimulated with either an innate immune system stimulant (LPS: lipopolysaccharide; 25 pg/ml), or a pan T cell stimulant (PHA: phytohemagglutinin; 1 μg/ml) for 24 h (Fig. 1). Initial stimulation prior to cell enrichment was carried out in unfractionated PBMCs to enable interactions to occur between myeloid/lymphoid cells to mimic in vivo conditions in which stimulation events occur in mixed cell environment. Unstimulated resting PBMC samples were also available. At the end of the culture period, non-adherent cells in suspension from resting and PHA-stimulated cultures were aspirated gently and enriched for CD4+ T cells (enriched T cells) using Dynabeads (Invitrogen) and stored in RNAprotect Cell reagent (Qiagen). Cells remaining in suspension in resting and LPS-stimulated cultures were aspirated, leaving an enriched population of monocytes and macrophages (enriched myeloid cells) adhered to the culture wells, and these were also resuspended vigorously into RNAprotect. Cell purities were not experimentally confirmed by flow cytometry because of the limited cell numbers available, and instead as a compromise we employed in silico analysis to estimate the abundances of relevant cell types from the resultant gene expression data using CIBERSORTx19. All cells in RNAprotect Cell reagent were banked at −80 °C.

The cells were thawed and centrifuged briefly for RNA extraction. Reagent was removed and total RNA was extracted from pelleted cells by an established in-house procedure using TRIzol (Life Technologies) in combination with RNEasy MinElute columns (Qiagen). The aqueous phase containing the RNA was then loaded onto an RNeasy MinElute column (Qiagen) to purify and concentrate the RNA. RNA quality was assessed on a Bioanalyzer 2100 using the RNA 6000 Nano kit (Agilent). There were 607 samples (one missing sample) in total from 152 individuals for gene expression profiling.

PCA of gene expression data showed the four clusters of samples of different cell types and conditions (Supplementary Fig. 15). For both deconvolution analysis (using web-based CIBERSORTx) and PCA, we used gene expression data that were quantile normalised across all 557 samples that passed QC (see the next section for the QC and normalisation pipeline), and the plots show the 494 samples from individuals with genotype data available that were included in the eQTL analysis.

### Gene expression profiling and data processing

Total RNA from four cell culture conditions (resting and LPS-stimulated myeloid cells, and resting and PHA-stimulated T cells) was quantified with Illumina HumanHT-12 v4 BeadChip gene expression array at the Genome Institute of Singapore. After excluding 31 samples with suspected cross-contamination or insufficient quantity of cDNA, 576 samples were successfully scanned. Supplementary Fig. 16 shows our QC and normalisation pipeline details. The raw microarray data and probe detection P-values were exported by the Illumina software GenomeStudio. We first removed three samples with zero intensity for almost all probes including negative controls and probes targeting housekeeping genes (two resting myeloid samples and one LPS-stimulated myeloid sample). We further removed 16 outlier samples (eight resting myeloid samples, five LPS-stimulated myeloid samples, and three resting T cell samples) with a low number of detectable probes (lying outside median ±2 × inter quartile range). Compared with other samples, these excluded samples had much lower intensity for positive controls including those targeting housekeeping genes.

After quality control, 557 samples remained for normalisation (resting monocyte/macrophage-enriched cultures: 130, LPS-stimulated monocyte/macrophage-enriched cultures: 141, resting T-cell-enriched cultures: 142, and PHA-stimulated T-cell-enriched cultures: 144). We performed background correction based on the intensity of the 770 negative control probes on the microarray, and then we performed quantile normalisation and log2 transformation within each cell type and condition using the neqc function from the limma (v3.36.5) R package58. We used updated probe annotation data and restricted the analysis to 33,436 reliable probes, excluding unaligned probes and probes aligned to multiple regions that were more than 25 bp apart59. Fifteen probes with missing data in ≥5 samples were removed. Detectable probes targeting autosomal genes (N = 20,532) were kept, comprising of probes with GenomeStudio detection P-values ≤ 0.01 in ≥2.5% of the samples from a specific condition group, or in ≥5% of all samples10. Gene annotation was obtained from the GENCODE release 19 (GRCh37 alignment, downloaded in October 2017). Among detectable probes, 19,230 had gene annotation in the GENCODE reference data. For genes that had multiple probes, we kept the probe with the highest mean intensity, resulting in 13,109 autosomal genes. For eQTL analysis, we performed a rank-based inverse normal transformation within each group, so that each gene expression followed a standard normal distribution.

### Genotyping and imputation

Genomic DNA was extracted from blood samples collected from 218 individuals. Genotyping was performed with Illumina Omni2.5 BeadChip array, with coverage of approximately 2.5 million markers. Variants with missing call rates >1%, MAF < 1%, or Hardy–Weinberg equilibrium (HWE) test P-value <1 × 10−6 were excluded, and individuals with missing call rates >1% were removed using Plink1.9. This produced an initial count of 1.4 million SNPs for 215 genotyped individuals. Of these, a total of 135 children also had gene expression data from cord blood (i.e. overlap with the 152 individuals described previously), and 106 and 119 individuals had gene expression data passing QC for both resting and stimulated conditions of monocyte/macrophage-enriched cultures and T-cell-enriched cultures, respectively, and 95 individuals had post-QC data for all four cultures.

We performed genotype imputation using the Michigan Imputation Server60 with Haplotype Reference Consortium (HRC) release r1.1 as the reference panel. Phasing was performed using Eagle v2.3 and genotype imputation was performed using Minimac3 v1.0.4. After filtering out variants with low imputation accuracy (R2 < 0.3), 12.7 million SNPs remained. For eQTL analysis, we focused on 4.3 million SNPs with MAFs ≥ 10%. The MAF cut-off used here was suggested by an eQTL simulation study in order to avoid inflated false positives in low-frequency variants given our limited sample size61.

### Cis-eQTL mapping and conditional analysis

To identify cis-eQTLs within each cell type and treatment group, we performed linear additive regression to model the effect of each SNP located within 1 Mb of the transcription start site (TSS) of the corresponding gene using the Matrix eQTL R package (v2.2)62. The sample size for eQTL mapping in each experimental condition was: 116 for resting myeloid cells, 125 for LPS-stimulated myeloid cells, 126 for resting T cells, and 127 for PHA-stimulated T cells. Genotype data were recoded as 0, 1, 2 based on the dosage of the HRC alternative allele. Gender, first three genotype PCs and first ten PEER63 factors capturing technical variation in transcriptomes were included as covariates in the linear model.

We applied a hierarchical correction procedure to correct for multiple testing61. Firstly, nominal P-values for all cis-SNPs from Matrix eQTL were adjusted by multiplying the number of effective independent SNPs for each gene (local correction), which was estimated by eigenMT based on genotype correlation matrix64. Secondly, the minimum locally adjusted P-value for each gene was kept and the FDR of significant genes was controlled at 5% using the Benjamini–Hochberg (BH) FDR-controlling procedure (global correction)65. Genes with global FDR ≤ 0.05 were considered significant eGenes. Thirdly, to obtain the list of significant eSNPs for each eGene, the locally adjusted minimum P-value corresponding to the global FDR threshold of 0.05 was calculated, and SNPs with a locally adjusted P-value lower than the threshold were considered significant eSNPs.

Next, we performed conditional analyses to identify additional independent eQTL signals for each eGene. The gene-level P-value nominal thresholds calculated in the hierarchical multiple-testing correction (eigenMT-BH) were used to determine significant associations: the locally adjusted minimum P-value corresponding to the global FDR threshold of 0.05 multiplied by the number of estimated independent SNPs for each gene. We used a two-step conditional analysis scheme as follows:

#### Forward stage

For each eGene, the number of independent cis-eQTL signals was learnt from an iterative procedure. We started from the top SNP with the minimum P-value for the eGene, which was added as a covariate in the linear model to test for cis-eQTLs. If any significant SNPs (with P-values smaller than the gene’s nominal threshold) were identified, the new top SNP identified in this iteration was added to the list of independent eQTL signals. In the next iteration of eQTL mapping, all previously identified eSNPs were adjusted for as covariates. The forward stage terminated if no additional significant associations were identified.

#### Backward stage

In this stage, the final list of significant SNPs representing each independent eQTL signal was determined. Let the list of independent SNPs for each eGene obtained from the forward stage be $$SNP_1,SNP_2,SNP_3, \ldots ,SNP_M$$, where M is the number of independent eQTL signals. Each of the independent eQTL signals was tested separately using a leave-out-one model adjusting for all other SNPs in the list as covariates. For example, when the ith eQTL signal was tested, $$SNP_1, \ldots ,SNP_{i - 1},SNP_{i + 1}, \ldots ,SNP_M$$ were added as covariates together with other covariates used in the original eQTL scan. The final set of independent eQTLs comprised of the eSNPs that remained significant in the backward stage.

For genes that had eQTLs in more than one experimental condition, we also applied conditional analyses to identify independent eQTL signals between conditions. More specifically, to determine whether two eQTL signals for an eGene identified in two experimental conditions were independent or the same signal, we adjusted for the top eSNP in one condition by adding it as a covariate in the linear model and performed eQTL scan again in the other condition. If any SNP was significant in the conditional model using the P-value threshold determined by the hierarchical correction procedure (eigenMT-BH), we considered these two eQTLs as independent signals. If none were significant in the conditional model, we considered it as shared eQTL signal between two conditions or lack of power to detect the independent signal.

### Comparison of neonatal vs adult cis-eQTLs

We downloaded full summary statistics of cis-eQTL analysis in adults from a response eQTL study in monocytes (Kim-Hellmuth et al. study11) and from the eQTL analysis in naïve T cells from the BLUERPRINT project22. Kim-Hellmuth et al. mapped cis-eQTLs in resting and LPS-stimulated CD14+ monocytes with two different durations of LPS: 90 min and 6 h, which were obtained from 134 adults aged from 18 to 35 years11. Chen et al. mapped cis-eQTLs in naïve CD4+ T cells from 169 adults (mean age of 55 years) from the BLUEPRINT project22. We could not find full summary statistics of any response eQTL analyses in stimulated T cells, so we only compared eQTLs in resting T cells.

To identify myeloid eQTLs that were specific to neonates, we analysed gene-SNP pairs (involving 10,749 genes) that had summary statistics available in both our study and the Kim-Hellmuth et al. study using the mashr (v0.2.21) R package20. Z scores were used as input. We used the most significant SNP for each gene as the strong set to learn data-driven covariance matrices, and randomly selected around nine tests for each gene as the random set to learn correlation structure among null tests and to fit the mash model. Posterior summaries were calculated for top eSNPs of each eGene using the fitted mash model, and calculated the local false sign rate (LFSR) for the most significant SNPs of 105 (out of 136) and 332 (out of 376) eGenes in resting and LPS-stimulated myeloid cells, respectively. Summary statistics for tests involving 7501 genes were available in both our study and the BLUEPRINT project, and we tested the most significant SNPs of 639 out of 971 eGenes identified in resting T cells. Neonate-specific eQTLs were defined as eQTLs that had LFDR < 0.05 in our dataset but not in cells from adults.

We have also estimated the sharing of cis-eQTLs between neonates and adults using the Storey and Tibshirani q-value approach23. $$\pi$$1 statistic was calculated to quantify the replication rate using the qvalue (v2.16.0) R package. We used the pi0est function to estimate the proportion of tests that were truly null ($$\pi$$0) among a list of P-values, given the assumption that P-values of truly null hypotheses should follow a uniform distribution. The proportion of true positive $$\pi$$1 was calculated as $$1 - \pi$$0. To estimate the sharing of eQTLs between two datasets, we focused on significant eQTL associations identified in one dataset, and used the corresponding P-values of the same list of tests (SNP-gene pairs) from the second dataset as input of the pi0est function.

### Enrichment analysis

We performed enrichment analyses using GARFIELD (version 2) to investigate the enrichment patterns of cis-eQTLs using predefined features such as genic annotations from ENCODE, GENCODE, and Roadmap Epigenomics project provided by this tool21. GARFIELD evaluates enrichment using generalised linear regression models that account for allele frequency, distance to the nearest gene TSS, and LD. LD correlation based on the UK10K dataset is also provided by the software. In each experimental condition, we used P-values for all SNPs tested in cis-eQTL analysis. If a SNP was tested for association with multiple genes, the smallest P-value was kept. Enrichment odds ratios were calculated at various eQTL significance thresholds: 1 × 10−3, 1 × 10−4, …, 1 × 10−7 (Supplementary Fig. 4), because GARFIELD accepts one single threshold.

### Response eQTL detection

Response eQTLs (reQTLs) were identified in myeloid cells and T cells separately. For each cell type, we focused on top eSNPs of eGenes that were significant in either resting or stimulated conditions. For eGenes that were significant in both conditions and for which two top eSNPs were not in high LD (r2 < 0.8), we tested both of the top eSNPs; on the other hand, if the two top eSNPs were in high LD, we tested the more significant one, to reduce tests on redundant SNPs. In myeloid cells, 417 interaction tests involving 398 eGenes were performed, and 1959 tests involving 1749 eGenes were performed in T cells. Gene expression data in two conditions were combined within each cell type, and the following linear mixed-effects model was tested for eGene–top eSNP pairs using the lmer function in the lme4 R package (v1.1-18-1)66:

$$y_i\sim x_i + c_i + x_i \, \times \, c_i + x_i^1 + \ldots + x_i^{14} + x_i^1 \times c_i + \ldots + x_i^{14} \, \times \, c_i + (1\left| {S_i} \right.).$$
(1)

where $$y_i$$ indicates the expression level of an eGene for the ith sample, $$x_i$$ the SNP allele dosage, $$c_i$$ the condition (resting: 0 and stimulated: 1) in which the gene expression was measured, $$x_i^1, \ldots x_i^{14}$$. the 14 covariates used in the original eQTL mapping (gender, three genotype PCs, and ten PEER factors), and $$S_i$$ the individual from which the ith sample was taken. The term $$x_i \times c_i$$ models the interaction between the genotype and the condition, and $$\left( {1\left| {S_i} \right.} \right)$$ indicates the individual-specific random effect for this paired study design.

We applied permutations to estimate empirical P-values for the interaction term. In each permutation step, the condition variable was shuffled within each individual, and the same linear mixed model was tested to get the permuted statistics for the interaction term12,67. The permutation-determined P-value for each interaction test was calculated as (s + 1)/(n + 1), where n was the total number of permutations (1000) and s was the number of cases where the permutated statistics were more significant than the original observed ones. We added 1 to both the numerator a the denominator to avoid underestimating permutation P-values. BH FDR-controlling procedure was applied to the permutation P-values and significant interactions were identified at 5% FDR.

### Trans-eQTL identification

To detect trans-acting genetic regulation of gene expression in each condition, we tested for associations between SNPs and genes that were located on different chromosomes using the same linear model and covariates as in the cis-eQTL mapping. We tried the following different approaches to deal with the multiple testing:

#### Genome-wide FDR correction

BH FDR-controlling procedure was applied to nominal P-values from all trans-association tests, and significant trans-associations were identified at 5% FDR6.

#### Gene-level FDR correction

For each gene, the P-value of the top SNP was multiplied by 1 × 106, which was the estimated number of independent SNPs across the genome (calculated as 0.05 divided by the commonly used genome-wide significance threshold of 5 × 10−8). To control gene-level FDR, a BH FDR-controlling procedure was then applied to the minimum adjusted P-values for all genes.

#### Gene-level Bonferroni correction

Bonferroni correction was used to control the gene-level FDR, by using a significance P-value threshold of 3.8 × 10−12 (5 × 10−8/13,109, where the denominator indicates the number of genes). The Bonferroni correction was extremely conservative because the tests (or genes) were not independent with each other.

In resting myeloid cells as well as in LPS-stimulated myeloid cells, one trans-eQTL signal was significant in all three methods. At 5% genome-wide FDR level, we observed 10 and 15 eGenes with significant trans-eQTLs (trans-eGenes) in resting and PHA-stimulated T cells, respectively, corresponding to a nominal P-value threshold of 1.9 × 10−10 in resting T cells and 2.5 × 10−10 in PHA-treated T cells. The number of significant trans-eGenes dropped, respectively, to six and eight by using the gene-level FDR correction (corresponding to a nominal P-value threshold of 5.3 × 10−12 in both conditions), and to five and seven by using the gene-level Bonferroni correction. The limited power was the major issue given the sample size; thus, we used genome-wide FDR correction, the least conservative method, to determine significant trans-eQTLs used in the downstream analysis6.

### Mediation analysis

We hypothesised that trans-eQTLs regulated the expression of distant genes through cis-mediators, or local genes whose expression was regulated by the same trans-eQTLs. To test this hypothesis, we focused on the trans-eQTLs that were also associated with adjacent cis-eGenes, meaning that the trans-eQTLs were also cis-eQTLs. For each trans-eGene–cis-eGene pair, we tested the trans-eSNP with the smallest P-value as the exposure, a cis-eGene as the mediator, and a trans-eGene as the outcome (Fig. 3b). In total, we tested 14 mediation trios: one from resting myeloid cells, one from LPS-stimulated myeloid cells, nine from resting T cells, and three from PHA-stimulated T cells.

We performed mediation test for the 14 trios using the mediation R package (v4.4.6)68. The effect of the exposure on the mediator ($$a$$) was estimated in cis-eQTL mapping. The effect of the mediator on the outcome ($$b$$) adjusting for the exposure and the effect of the exposure on the outcome ($$c{\prime}$$) adjusting for the mediator were estimated in the following multiple regression:

$$y_i\sim x_i + x_i^{{\mathrm{cis}}} + x_i^1 + \ldots + x_i^{14}.$$
(2)

where $$y_i$$ indicates the value of the outcome (or the expression level of the trans-eGene) for the ith sample, $$x_i$$ the exposure (or the eSNP allele dosage), $$x_i^{{\mathrm{cis}}}$$ the mediator (or the cis-eGene expression), and $$x_i^1, \ldots x_i^{14}$$ the 14 covariates used in eQTL mapping. The estimates of $$b$$ and $$c{\prime}$$ were beta coefficients for $$x_i^{{\mathrm{cis}}}$$ and $$x_i$$, respectively. The direct effect of the exposure on the outcome was quantified as $$c{\prime}$$, the indirect effect of the exposure on the outcome through the mediator was quantified as $$a \times b$$, and the total effect was the sum of the previous two effects. Complete mediation occurs when the direct effect $$c{\prime}$$ is zero after controlling for the mediator, and partial mediation happens when the direct effect is different from zero. To identify significant mediation trios (the null hypothesis $$H_0:ab = 0$$), we used a nonparametric bootstrap method (10,000 simulations) implemented in the mediation R package for variance estimation and P-value calculation. BH FDR-controlling procedure was applied to correct for multiple testing.

### Genetic overlap of eQTLs and diseases

We downloaded publicly-available GWAS data for the following immune-mediated diseases: allergic disease (asthma, hay fever, or eczema), allergic rhinitis, allergic sensitisation, asthma (childhood-onset and adult-onset asthma), inflammatory bowel disease including its two subtypes—Crohn’s disease and ulcerative colitis, celiac disease, autoimmune thyroid disease, juvenile idiopathic arthritis, multiple sclerosis, narcolepsy, primary biliary cirrhosis, primary sclerosing cholangitis, psoriasis, rheumatoid arthritis, systemic lupus erythematosus, and type 1 diabetes. We also analysed the summary statistics from a GWAS of educational attainment as a negative control. References of the GWAS studies are in Supplementary Information. These datasets contained summary statistics obtained using European populations for both significant and non-significant genetic variants, and GRCh37 genomic coordinates were available.

We performed enrichment tests for each of the four sets of significant eQTLs using GARFIELD21. Generalised linear models were applied to test for enrichment in eQTLs of variants associated with the above diseases at a significance threshold of 1 × 10−6. Bonferroni correction was applied to correct for multiple testing, where the number of tests was the number of GWAS datasets (24) multiplied by the number of eQTL datasets (4), and the Bonferroni-adjusted P-value threshold was 5.0 × 10−4, adjusting for the 4 × 25 (100) tests.

### Colocalisation of cis-eQTLs with disease associations

We applied a Bayesian method implemented in the coloc R package (v3.1)25 to test whether any of the disease-associated GWAS loci shared the same causal variants with the cis-eQTLs. Full summary statistics were required to run the colocalisation analysis using coloc. For loci where cis-eQTLs were also associated with diseases at a P-value threshold of 1 × 10−6, colocalisation test was performed on a 400-kb window centered on the top cis-eSNP. For each locus, colocalisation test was performed on overlapping SNPs where both eQTL and GWAS summary statistics were available. We excluded regions where not enough SNPs (<25) were available for colocalisation test. Selection of different prior probabilities of a SNP being causal for both of the traits affects the posterior support for colocalisation. To be conservative, we used a lower prior probability of 1 × 10−6 instead of the default value of 1 × 10−5.

For each locus, the Bayesian method assessed the support for the following five exclusive hypotheses: no causal variants for either of the two traits (H0), a causal variant for gene expression only (H1), a causal variant for disease risk only (H2), distinct causal variants for two traits (H3), and the same shared causal variant for both traits (H4). The package estimated posterior probabilities (PP0, PP1, PP2, PP3, PP4) to summarise the evidence for the above five hypotheses. High PP1 or PP2 and low PP3 + PP4 indicate a lack of power to identify the causal signals25. We excluded loci where PP3 + PP4 < 0.8, and focused on loci with strong evidence support for shared causal variants (H4), i.e. ratio of PP4 to PP3 ≥ 5.

To assess if our significant colocalisations were robust across a range of different p12 priors (from 1 × 10−9 to 1 × 10−5), we performed the sensitivity analysis using the sensitivity function in a newer version of the coloc R package (v4.0). All our significant colocalisations passed the sensitivity analysis, and PP4 values using different priors can be found in Supplementary Data 4.

### Mendelian randomisation analysis

To investigate causal effects of eGene expression on the above immune-mediated diseases, we performed a two-sample Mendelian randomisation (MR) analysis. Summary statistics from both our eQTL and external GWAS studies were required, including beta coefficient and its standard error, effective allele (based on which the beta was estimated), the other allele, and P-value. For each disease trait, we used cis-eSNPs that were also included in the GWAS dataset, and removed ambiguous variants (if any) using the TwoSampleMR R package (v0.4.14)69. We then selected LD pruned (r2 < 0.1) cis-eSNPs as genetic instrumental variables (IVs). We focused on eGenes with at least three genetic IVs available, and performed the following MR methods implemented in the MendelianRandomization R package (v0.4.1)70: inverse variance weighted (IVW), weighted median, weighted mode, and MR Egger. These methods have different assumptions for valid IVs: IVW assumes that all IVs are valid; weighted median assumes that valid IVs contribute to more than 50% of the weight; weighted mode assumes that the largest group of IVs are valid; MR Egger regression, which is the least sensitive, assumes that the pleiotropic effects of IVs are not correlated with the genetic effects on exposure. We excluded the causal associations for which the intercept in the MR Egger method was significantly not equal to 0, indicating significant average pleiotropic effects. Gene expression was considered to have suggestive evidence of causal effects when at least three out of the four methods provided significant P-value (≤0.05). In the ‘Results’ section, we reported the statistics of the weighted mode method, which has the least assumption among all methods except the MR Egger, but is more sensitive than MR Egger.

### Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

## Data availability

The raw microarray data, normalised gene expression data, and full summary statistics of the eQTL and response eQTL analyses have been deposited in ArrayExpress with the accession code E-MTAB-8977. GWAS summary statistics used in this study are from the following publicly available sources: ImmunoBase (https://www.immunobase.org/), LD Hub (http://ldsc.broadinstitute.org/ldhub/), and GWAS Catalog (https://www.ebi.ac.uk/gwas/). The transcriptome profiles used to infer cell type abundances (CIBERSORTx) are available on https://cibersortx.stanford.edu. The GENCODE reference data are available at https://www.gencodegenes.org. The Haplotype Reference Consortium (HRC) r1.1 imputation reference panel was available at https://imputationserver.sph.umich.edu/.

## Code availability

The analysis scripts for eQTL mapping, colocalisation, and Mendelian Randomisation are available at https://github.com/QinqinHuang/CAS_eQTL.

## References

1. 1.

Postma, D. S., Bush, A. & van den Berge, M. Risk factors and early origins of chronic obstructive pulmonary disease. Lancet 385, 899–909 (2015).

2. 2.

Barker, D. J. The origins of the developmental origins theory. J. Intern Med. 261, 412–417 (2007).

3. 3.

Gensollen, T., Iyer, S. S., Kasper, D. L. & Blumberg, R. S. How colonization by microbiota in early life shapes the immune system. Science 352, 539–544 (2016).

4. 4.

Reynolds, L. A. & Finlay, B. B. Early life factors that affect allergy development. Nat. Rev. Immunol. 17, 518–528 (2017).

5. 5.

Carraro, S., Scheltema, N., Bont, L. & Baraldi, E. Early-life origins of chronic respiratory diseases: understanding and promoting healthy ageing. Eur. Respir. J. 44, 1682–1696 (2014).

6. 6.

GTEx Consortium et al. Genetic effects on gene expression across human tissues. Nature 550, 204–213 (2017).

7. 7.

Schmiedel, B. J. et al. Impact of genetic polymorphisms on human immune cell gene expression. Cell 175, e16 (2018).

8. 8.

Peng, S. et al. Expression quantitative trait loci (eQTLs) in human placentas suggest developmental origins of complex diseases. Hum. Mol. Genet 26, 3432–3441 (2017).

9. 9.

O’Brien, H. E. et al. Expression quantitative trait loci in the developing human brain and their enrichment in neuropsychiatric disorders. Genome Biol. 19, 194 (2018).

10. 10.

Fairfax, B. P. et al. Innate immune activity conditions the effect of regulatory variants upon monocyte gene expression. Science 343, 1246949 (2014).

11. 11.

Kim-Hellmuth, S. et al. Genetic regulatory effects modified by immune activation contribute to autoimmune disease associations. Nat. Commun. 8, 266 (2017).

12. 12.

Alasoo, K. et al. Shared genetic effects on chromatin and gene expression indicate a role for enhancer priming in immune response. Nat. Genet. 50, 424–431 (2018).

13. 13.

Lee, M. N. et al. Common genetic variants modulate pathogen-sensing responses in human dendritic cells. Science 343, 1246980 (2014).

14. 14.

Barreiro, L. B. et al. Deciphering the genetic architecture of variation in the immune response to Mycobacterium tuberculosis infection. Proc. Natl Acad. Sci. USA 109, 1204–1209 (2012).

15. 15.

Ye, C. J. et al. Intersection of population variation and autoimmunity genetics in human T cell activation. Science 345, 1254665 (2014).

16. 16.

Kusel, M. M. et al. Role of respiratory viruses in acute upper and lower respiratory tract illness in the first year of life: a birth cohort study. Pediatr. Infect. Dis. J. 25, 680–686 (2006).

17. 17.

Kusel, M. M. et al. Early-life respiratory viral infections, atopic sensitization, and risk of subsequent development of persistent asthma. J. Allergy Clin. Immunol. 119, 1105–1110 (2007).

18. 18.

Teo, S. M. et al. Airway microbiota dynamics uncover a critical window for interplay of pathogenic bacteria and allergy in childhood respiratory disease. Cell Host Microbe 24, e5 (2018).

19. 19.

Newman, A. M. et al. Determining cell type abundance and expression from bulk tissues with digital cytometry. Nat. Biotechnol. 37, 773–782 (2019).

20. 20.

Urbut, S. M., Wang, G., Carbonetto, P. & Stephens, M. Flexible statistical methods for estimating and testing effects in genomic studies with multiple conditions. Nat. Genet 51, 187–195 (2019).

21. 21.

Iotchkova, V. et al. GARFIELD classifies disease-relevant genomic features through integration of functional annotations with association signals. Nat. Genet. 51, 343–353 (2019).

22. 22.

Chen, L. et al. Genetic Drivers of Epigenetic and Transcriptional Variation in Human Immune Cells. Cell 167, e24 (2016).

23. 23.

Storey, J. D. & Tibshirani, R. Statistical significance for genomewide studies. Proc. Natl Acad. Sci. USA 100, 9440–9445 (2003).

24. 24.

Bryois, J. et al. Cis and trans effects of human genomic variants on gene expression. PLoS Genet. 10, e1004461 (2014).

25. 25.

Giambartolomei, C. et al. Bayesian test for colocalisation between pairs of genetic association studies using summary statistics. PLoS Genet 10, e1004383 (2014).

26. 26.

Roychoudhuri, R. et al. BACH2 regulates CD8(+) T cell differentiation by controlling access of AP-1 factors to enhancers. Nat. Immunol. 17, 851–860 (2016).

27. 27.

Shinnakasu, R. et al. Regulated selection of germinal-center cells into the memory B cell compartment. Nat. Immunol. 17, 861–869 (2016).

28. 28.

Afzali, B. et al. BACH2 immunodeficiency illustrates an association between super-enhancers and haploinsufficiency. Nat. Immunol. 18, 813–823 (2017).

29. 29.

Roychoudhuri, R. et al. BACH2 represses effector programs to stabilize T(reg)-mediated immune homeostasis. Nature 498, 506–510 (2013).

30. 30.

Schutyser, E., Struyf, S. & Van Damme, J. The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 14, 409–426 (2003).

31. 31.

Merk, M., Mitchell, R. A., Endres, S. & Bucala, R. D-dopachrome tautomerase (D-DT or MIF-2): doubling the MIF cytokine family. Cytokine 59, 10–17 (2012).

32. 32.

Peters, J. E. et al. Insight into genotype-phenotype associations through eQTL mapping in multiple cell types in health and immune-mediated disease. PLoS Genet. 12, e1005908 (2016).

33. 33.

Pierce, B. L. et al. Mediation analysis demonstrates that trans-eQTLs are often explained by cis-mediation: a genome-wide analysis among 1,800 South Asians. PLoS Genet. 10, e1004818 (2014).

34. 34.

Yang, F., Wang, J., Consortium, G. T., Pierce, B. L. & Chen, L. S. Identifying cis-mediators for trans-eQTLs across many human tissues using genomic mediation analysis. Genome Res. 27, 1859–1871 (2017).

35. 35.

Ferretti, M. B., Ghalei, H., Ward, E. A., Potts, E. L. & Karbstein, K. Rps26 directs mRNA-specific translation by recognition of Kozak sequence elements. Nat. Struct. Mol. Biol. 24, 700–707 (2017).

36. 36.

Min, E. E., Roy, B., Amrani, N., He, F. & Jacobson, A. Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit. RNA 19, 1105–1115 (2013).

37. 37.

Cui, D. et al. The ribosomal protein S26 regulates p53 activity in response to DNA damage. Oncogene 33, 2225–2235 (2014).

38. 38.

Demedts, I. K. et al. Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 175, 998–1005 (2007).

39. 39.

Faiz, A. et al. Profiling of healthy and asthmatic airway smooth muscle cells following interleukin-1beta treatment: a novel role for CCL20 in chronic mucus hypersecretion. Eur. Respir. J. 52,1800310 (2018).

40. 40.

Kim, S., Lewis, C. & Nadel, J. A. CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells. J. Immunol. 186, 3392–3400 (2011).

41. 41.

Francis, J. N., Sabroe, I., Lloyd, C. M., Durham, S. R. & Till, S. J. Elevated CCR6+CD4+ T lymphocytes in tissue compared with blood and induction of CCL20 during the asthmatic late response. Clin. Exp. Immunol. 152, 440–447 (2008).

42. 42.

de Waal Malefyt, R. et al. Differential regulation of IL-13 and IL-4 production by human CD8+ and CD4+ Th0, Th1 and Th2 T cell clones and EBV-transformed B cells. Int Immunol. 7, 1405–1416 (1995).

43. 43.

Punnonen, J. et al. Interleukin 13 induces interleukin 4-independent IgG4 and IgE synthesis and CD23 expression by human B cells. Proc. Natl Acad. Sci. USA 90, 3730–3734 (1993).

44. 44.

Wills-Karp, M. et al. Interleukin-13: central mediator of allergic asthma. Science 282, 2258–2261 (1998).

45. 45.

Berry, M. A. et al. Sputum and bronchial submucosal IL-13 expression in asthma and eosinophilic bronchitis. J. Allergy Clin. Immunol. 114, 1106–1109 (2004).

46. 46.

Saha, S. K. et al. Increased sputum and bronchial biopsy IL-13 expression in severe asthma. J. Allergy Clin. Immunol. 121, 685–691 (2008).

47. 47.

Tsilogianni, Z. et al. Sputum interleukin-13 as a biomarker for the evaluation of asthma control. Clin. Exp. Allergy 46, 1498 (2016).

48. 48.

Guo, Y. & Wang, A. Y. Novel immune check-point regulators in tolerance maintenance. Front Immunol. 6, 421 (2015).

49. 49.

Le Page, C. et al. BTN3A2 expression in epithelial ovarian cancer is associated with higher tumor infiltrating T cells and a better prognosis. PLoS ONE 7, e38541 (2012).

50. 50.

Vavassori, S. et al. Butyrophilin 3A1 binds phosphorylated antigens and stimulates human gammadelta T cells. Nat. Immunol. 14, 908–916 (2013).

51. 51.

Vantourout, P. et al. Heteromeric interactions regulate butyrophilin (BTN) and BTN-like molecules governing gammadelta T cell biology. Proc. Natl Acad. Sci. USA 115, 1039–1044 (2018).

52. 52.

Lamontagne, M. et al. Leveraging lung tissue transcriptome to uncover candidate causal genes in COPD genetic associations. Hum. Mol. Genet. 27, 1819–1829 (2018).

53. 53.

Nair, R. P. et al. Sequence and haplotype analysis supports HLA-C as the psoriasis susceptibility 1 gene. Am. J. Hum. Genet. 78, 827–851 (2006).

54. 54.

Genetic Analysis of Psoriasis, C. et al. A genome-wide association study identifies new psoriasis susceptibility loci and an interaction between HLA-C and ERAP1. Nat. Genet. 42, 985–990 (2010).

55. 55.

Breen, M. S. et al. Gene expression in cord blood links genetic risk for neurodevelopmental disorders with maternal psychological distress and adverse childhood outcomes. Brain Behav. Immun. 73, 320–330 (2018).

56. 56.

Green, B. B. & Marsit, C. J. Select prenatal environmental exposures and subsequent alterations of gene-specific and repetitive element DNA methylation in fetal tissues. Curr. Environ. Health Rep. 2, 126–136 (2015).

57. 57.

Petit, T. et al. Detection of maternal cells in human fetal blood during the third trimester of pregnancy using allele-specific PCR amplification. Br. J. Haematol. 98, 767–771 (1997).

58. 58.

Ritchie, M. E. et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43, e47 (2015).

59. 59.

Arloth, J., Bader, D. M., Roh, S. & Altmann, A. Re-annotator: annotation pipeline for microarray probe sequences. PLoS ONE 10, e0139516 (2015).

60. 60.

Das, S. et al. Next-generation genotype imputation service and methods. Nat. Genet. 48, 1284–1287 (2016).

61. 61.

Huang, Q. Q., Ritchie, S. C., Brozynska, M. & Inouye, M. Power, false discovery rate and Winner’s Curse in eQTL studies. Nucleic Acids Res. 46, e133 (2018).

62. 62.

Shabalin, A. A. Matrix eQTL: ultra fast eQTL analysis via large matrix operations. Bioinformatics 28, 1353–1358 (2012).

63. 63.

Stegle, O., Parts, L., Piipari, M., Winn, J. & Durbin, R. Using probabilistic estimation of expression residuals (PEER) to obtain increased power and interpretability of gene expression analyses. Nat. Protoc. 7, 500–507 (2012).

64. 64.

Davis, J. R. et al. An efficient multiple-testing adjustment for eQTL studies that accounts for linkage disequilibrium between variants. Am. J. Hum. Genet. 98, 216–224 (2016).

65. 65.

Benjamini, Y. & Hochberg, Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Stat. Soc. B 57, 289–300 (1995).

66. 66.

Bates, D., Machler, M., Bolker, B. M. & Walker, S. C. Fitting linear mixed-effects models using lme4. J. Stat. Softw. 67, 1–48 (2015).

67. 67.

Davenport, E. E. et al. Discovering in vivo cytokine-eQTL interactions from a lupus clinical trial. Genome Biol. 19, 168 (2018).

68. 68.

Tingley, D., Yamamoto, T., Hirose, K., Keele, L. & Imai, K. mediation: R Package for causal mediation analysis. J. Stat. Softw. 59, 1–38 (2014).

69. 69.

Hemani, G. et al. The MR-Base platform supports systematic causal inference across the human phenome. Elife 7, e34408 (2018).

70. 70.

Yavorska, O. O. & Burgess, S. MendelianRandomization: an R package for performing Mendelian randomization analyses using summarized data. Int J. Epidemiol. 46, 1734–1739 (2017).

## Acknowledgements

We thank the participants, their parents, and project staff of the Childhood Asthma Study. This research was supported by the NHMRC of Australia (project grant no. 1049539 to M.I. and K.E.H., Fellowships 1061409 to K.E.H., and 1061435 to M.I.). This research was supported by core funding from: the UK Medical Research Council (MR/L003120/1), the British Heart Foundation (RG/13/13/30194; RG/18/13/33946) and the National Institute for Health Research [Cambridge Biomedical Research Centre at the Cambridge University Hospitals NHS Foundation Trust]. This research was supported in part by the Victorian Government’s Operational Infrastructure Support Program and the UK National Institute of Health Research. M.I. and S.C.R. are funded by the National Institute for Health Research [Cambridge Biomedical Research Centre at the Cambridge University Hospitals NHS Foundation Trust]. This work was supported by Health Data Research UK, which is funded by the UK Medical Research Council, Engineering and Physical Sciences Research Council, Economic and Social Research Council, Department of Health and Social Care (England), Chief Scientist Office of the Scottish Government Health and Social Care Directorates, Health and Social Care Research and Development Division (Welsh Government), Public Health Agency (Northern Ireland), British Heart Foundation and Wellcome. K.E.H. is supported by a Senior Medical Research Fellowship from the Viertel Foundation of Australia. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health and Social Care.

## Author information

Authors

### Contributions

M.I. conceived this project and supervised the work. K.E.H. and P.G.H. supervised the work. P.G.H. and P.D.S. are the principle investigators of the Childhood Asthma Study. Q.Q.H. carried out all analyses and wrote the paper. H.H.F.T. carried out quality control of the genotype data and Q.Q.H. carried out genotype imputation. M.I, H.H.F.T., and S.M.T. helped writing and editing the paper. H.H.F.T. contributed to the biological interpretation. H.H.F.T. and S.M.T. helped with quality control and data analysis. D.M. performed the stimulation experiments and RNA extraction. S.C.R. provided the codes for the Mendelian randomisation analysis and helped with the interpretation. A.P.N. helped with quality control of the microarray data, and the colocalisation analysis and its interpretation. M.B. was the project manager. A.S. contributed to the interaction model of the reQTL analysis. A.B. helped with quality control and normalisation of the microarray gene expression data. B.J.H. helped with lab work. C.C.K. contributed to the quantification of microarray gene expression data. All authors approved the paper.

### Corresponding authors

Correspondence to Qin Qin Huang or Michael Inouye.

## Ethics declarations

### Competing interests

The authors declare no competing interests.

Peer review information Nature Communications thanks Rachel Freathy and the other, anonymous reviewer(s) for their contribution to the peer review of this work. Peer review reports are available.

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

## Rights and permissions

Reprints and Permissions

Huang, Q.Q., Tang, H.H.F., Teo, S.M. et al. Neonatal genetics of gene expression reveal potential origins of autoimmune and allergic disease risk. Nat Commun 11, 3761 (2020). https://doi.org/10.1038/s41467-020-17477-x