Intermolecular [3+3] ring expansion of aziridines to dehydropiperi-dines through the intermediacy of aziridinium ylides


The importance of N-heterocycles in drugs has stimulated diverse methods for their efficient syntheses. Methods that introduce significant stereochemical complexity are attractive for identifying new bioactive amine chemical space. Here, we report a [3 + 3] ring expansion of bicyclic aziridines and rhodium-bound vinyl carbenes to form complex dehydropiperidines in a highly stereocontrolled rearrangement. Mechanistic studies and DFT computations indicate that the reaction proceeds through formation of a vinyl aziridinium ylide; this reactive intermediate undergoes a pseudo-[1,4]-sigmatropic rearrangement to directly furnish heterocyclic products with net retention at the new C-C bond. In combination with asymmetric silver-catalyzed aziridination, enantioenriched scaffolds with up to three contiguous stereocenters are rapidly delivered. The mild reaction conditions, functional group tolerance, and high stereospecificity of this method are well-suited for appending piperidine motifs to natural product and complex molecules. Ultimately, our work establishes the value of underutilized aziridinium ylides as key intermediates for converting small, strained rings to larger N-heterocycles.


The importance of nitrogenated heterocycles in pharmaceuticals, natural products, and fine chemicals continues to drive innovative strategies for their efficient syntheses from readily available precursors1,2,3,4,5,6,7,8,9,10,11,12,13,14. The ability to improve upon existing preparations of known compounds, enable alternate retrosynthetic approaches to useful building blocks, and increase opportunities to explore novel chemical space outside of ‘flatland’ are all compelling reasons to develop new approaches to N-heterocycles15,16,17,18,19,20. Piperidines rank as the most prominent N-heterocyclic pharmacophore in current drugs on the market, appearing in ~55% of all FDA-approved drugs containing at least one N-heterocycle, as well as in numerous bioactive natural products3. Convergent methods that unite multiple reactive fragments, particularly hetero-[4+2] cycloadditions, deliver substituted dehydropiperidines in enantioenriched form21,22,23,24,25,26,27,28,29,30,31; however, critical substrate and/or catalyst control over regio- and stereoselectivity is challenging and often results in narrow scope. Traditional preparations of stereodefined piperidines using intramolecular SN2-type reactions require selective installation of functional groups prior to ring closure, resulting in lower efficiency, modularity, and step economy as the desired target’s complexity increases3. A less common approach is to engage an aziridine and a simple coupling partner in a stereocontrolled, and ideally stereospecific, ring expansion reaction.

Aziridines are an ideal starting material for conversion into larger nitrogenated heterocycles. They are easily accessible from a variety of alkenes by nitrene transfer or from simple manipulations of epoxides. In addition, methods for asymmetric aziridination enable these strained rings to be prepared with substantial stereochemical and substitutional complexity32,33,34,35,36. An attractive feature of aziridines is their ~26 kcal mol−1 of ring strain, ensuring a favorable thermodynamic driving force for ring-opening. Figure 1 illustrates a selection of transition metal-catalyzed aziridine expansions that furnish 4–7 membered N-heterocycles37,38,39,40,41,42,43,44,45,46,47,48. For example, Alper and others have achieved metal-catalyzed carbonylations of aziridines to yield valuable β-lactams, although good regioselectivity depends on the substitution pattern of the three-membered ring45. Njardarson has described a series of Cu-catalyzed transformations of aziridines to pyrrolidines and related rings; while these reactions are often stereospecific, they are largely limited to intramolecular examples40,41,42. Scattered examples describing the conversion of aziridines to piperidines, dehydropiperidines, and azepines (Fig. 1a) are known, but these reactions are also intramolecular or have limited scope44,46,47. In contrast, we were encouraged by Rowlands report of a single example of the formation of a dehydropiperidine in 21% yield (Fig. 1b) from a vinylaziridine49. This reaction presumably occurs through intramolecular formation of an aziridinium ylide, followed by [2,3]-rearrangement; however, only one invertomer undergoes the desired cyclization. The low yield is ascribed to the required coplanarity of the anion and the vinyl group; if the ylide forms with the opposite stereochemistry at nitrogen, [1,5]-hydrogen shifts and other decomposition50 pathways compete with productive ring expansion.

Fig. 1: Transformations of aziridines to N-heterocycles.

a Typical transition metal-catalyzed ring expansions. b Cu-catalyzed aziridine ring expansion through the intermediacy of an aziridinium ylide.

As noted in Fig. 1, aziridines must often be highly engineered to achieve effective ring expansions. In particular, inclusion of an adjacent vinyl group provides the kinetic impetus to drive successful metal-mediated isomerizations and other functionalizations39,40,41,44,46,47. In contrast, utilizing unbiased aziridines for stereocontrolled expansions to larger N-heterocycles can be challenging, as epimerization or racemization of the aziridine must be avoided to successfully relay stereochemical information at sp3 stereocenters to the product with excellent fidelity51,52,53,54. Despite these difficulties, the ease of aziridine preparation and their strain-loaded reactivity make them attractive scaffolds for the discovery of new reactivity32,33,34,35,36.

We previously leveraged the unusual strain (~42 kcal/mol) in methyleneaziridine 1a to achieve a formal [3+1] reaction to furnish methyleneazetidine 1d upon exposure to rhodium-supported carbene 1b (Fig. 2a)55,56,57,58,59,60,61,62. Mechanistic studies support initial formation of the aziridinium ylide 1c, which subsequently undergoes a highly asynchronous, concerted [2,3]-Stevens rearrangement to form 1d63. The complete transfer of the chirality in 1ad provides further experimental evidence to support this mechanism. This efficient transformation, which forms new C–C and C–N bonds and two adjacent stereocenters in a stereospecific intermolecular two-fragment coupling, prompted us to undertake further studies of aziridinium ylides to extend the scope to unbiased aziridines. Unfortunately, removing the exocyclic alkene of 1a in 1e (Fig. 2b) gave only cheletropic extrusion with 1b to furnish 1g, a pathway also observed by Watanabe in Cu-catalyzed reaction of aziridines with ethyldiazoacetate50. We reasoned appending a vinyl group to the carbene precursor 1i (Fig. 2c) could facilitate the desired ring expansion of aziridinium ylide 1j–k over competing cheletropic extrusion to 1l. This transformation represents a net [3+3] annulation of a vinyl carbenoid and a bicyclic aziridine; depending on the mechanism, transfer of stereochemical information from the aziridine to the dehydropiperidine with good fidelity could be envisaged. Herein, we report an attractive strategy to assemble stereochemically complex and highly substituted dehydropiperidines via an intermolecular ring expansion between simple bicyclic aziridines and Rh-supported vinyl carbenes. The intermediacy of an unusual aziridinium ylide species is followed by a stereospecific rearrangement that secures access to enantioenriched products.

Fig. 2: Reaction design.

a Methyleneazetidines from ring expansion of aziridines. b Competing cheletropic extrusion pathways. c Favoring ring expansion over cheletropic extrusion for the synthesis of dehydropiperidines.


Scope of the [3+3] ring expansion

To our delight, reaction of cis-alkene-derived aziridine 2a (Table 1) with Davies’ styrenyl diazoacetate64 3a under dirhodium catalysis produced 4aa in 75% yield and in excellent >19:1 diastereoselectivity. Optimization of conditions for the [3+3] aziridine ring expansion revealed the dirhodium paddlewheel complex Rh2(OAc)4 was the superior catalyst using slow addition of the vinyl diazoacetate (see the Supplementary Information for further details). With these conditions in hand, the aziridine scope was further explored using 3a as the vinyl carbene precursor. Linear alkyl groups on aziridines 2a–d, including benzyl, methyl, ethyl, and n-butyl, gave good yields of the products 4aa–da in high dr. 1H NMR spectroscopy indicated a dr of at least >19:1 for the dehydropiperidines, with only trace amounts of the presumed diastereomers noted. Increasing the bulk of the substituent on the aziridine to an isopropyl group in 2e furnished 4ea in 71% yield and excellent dr. Alkyl chloride and ether functionalities were also well tolerated to deliver dehydropiperidines 4fa and 4ga. Alkyl substitution α to the carbamate tether in 2h (dr > 19:1) gave a 74% yield of 4ha as a single diastereomer. Aziridine 2i, unsubstituted at the terminal carbon, gave 4ia in 67% yield and >19:1 dr. Finally, it was not necessary to have the carbamate contained in a six-membered ring, as the [5.3]-bicyclic aziridine 2j (R1 = Et, n = 0) gave the [5.6]-bicyclic ring 4ja in 42% yield and in >19:1 dr.

Table 1 Scope of the aziridine and diazoester in Rh-catalyzed ring expansions to dehydropiperidines.

We next examined the scope of the carbene precursor using ethyl-substituted aziridine 2c (Table 1). The impact of the electronics of a series of phenyl-substituted diazo acetates 3a–f was investigated first. Similar yields were obtained for 4ca and 4cb–cc, irrespective of whether the diazoester carbene precursor contains electron-donating or neutral substituents, highlighting there is little effect of the styrene electronics on the reaction outcome. A single-crystal X-ray structure of 4cc established the relative stereochemical configuration of the heterocycle product (see expansion for 4cc and the Supplementary Information for further details), corroborating nOe studies. Moving the Br to the meta position in 3d resulted in a similar 79% yield of 4cd. Diazoester 3e, bearing a strongly electron-withdrawing trifluoromethyl group, gave a 69% yield of 4ce, also in good dr, providing a convenient way to introduce valuable fluorines into the dehydropiperidine products.

Carbene transfer of the naphthyl-substituted 3f provided 4cf in similar yield and dr as compared with 4ca. It was not necessary to employ a styrenyl-derived diazoester, as a series of β-alkyl-substituted diazoesters 3g–i all resulted in good yields of unsaturated piperidines 4cgci as single diastereomers as determined by 1H NMR. The furan-substituted 3j gave 4cj in only 14% isolated yield, as these oxygen heterocycles have been reported to be reactive in the presence of metal-supported carbenoids65,66. Bulking up the methyl ester on the diazoester to a cyclohexyl ester was also successful, producing 4ck in 56% yield and >19:1 dr.

Computational studies of the mechanism

The difficulty of studying carbene transfer using traditional kinetics, particularly when slow addition is required, led us to turn to DFT calculations (computational details are in the Supplementary Information and Supplementary Data 1) to gain more insight into the mechanism of the ring expansion (Fig. 3). The reaction of the methyl-substituted aziridine 2b and the [Rh2]-carbenoid derived from diazoacetate 3a to furnish 4ba was explored computationally. Nucleophilic attack of the aziridine nitrogen atom on the carbon of the Rh-supported carbenoid occurs as the first step to form aziridinium ylide INT1 via transition state TS162,63. Then, barrierless and exergonic dissociation of the dirhodim catalyst from the nitrogen produces zwitterion INT2, where the negative charge is fully delocalized into the allylic system (Fig. 3b).

Fig. 3: Mechanistic studies of the [3+3] ring expansion.

a Computed reaction profile for the process involving 2b and Rh2-bound carbene 3a-Rh2. Relative free energies (ΔG, computed at 298.15 K and 1 M) and bond distances are in kcal/mol and Å, respectively. All data are computed at the SMD(CH2Cl2)-B3LYP-D3/def2-SVP level. Values within parentheses are computed at the SMD(CH2Cl2)-B3LYP-D3/def2-TZVPP//SMD(CH2Cl2)-B3LYP-D3/def2-SVP level of theory. b Stereochemical retention experiment.

Two fates were envisaged for INT2. In the first case, INT2 forms azadiene INT3 through a cheletropic extrusion (via TS2), followed by a concerted aza-Diels–Alder cycloaddition (via TS3) to furnish 4ba. However, subjecting enantioenriched (S,R)-2a67 to the standard reaction conditions resulted in transfer of the chirality to (R,R,R)-4aa with good fidelity (Fig. 3b). This effectively rules out cheletropic extrusion, followed by aza-Diels–Alder reaction, as the operative mechanism for formation of the dehydropiperidine. We also considered that Stevens rearrangements involving ammonium ylides are occasionally reported to proceed through diradical intermediates68; however, the chirality transfer from (S,R)-2a to (R,R,R)-4aa suggests that a diradical pathway cannot be operative in the absence of a solvent cage.

An alternate fate for the aziridinium ylide INT2 is suggested by the computations in Scheme 3 and involves a rare pseudo-[1,4]-sigmatropic rearrangement of INT2 via TS2′. This pathway has never been observed for an aziridinium ylide; however, a single example of a [1,4]-rearrangement of an ammonium benzylide has been reported69,70. According to our calculations, this path proceeds with a lower barrier than the competing cheletropic extrusion (∆∆G = 5.3 kcal/mol). In addition, this mechanism would be expected to directly parlay absolute and relative stereochemical information from the aziridine into the product, supporting our experimental observations of chirality transfer from (S,R)-2a to (R,R,R)-4aa62,63. Zwitterion INT3′ could not be located on the potential energy surface. Indeed, intrinsic reaction calculations (IRCs) starting from TS2′ show the final closing of the C–C bond to form the piperidine exists on a plateau-like energy pathway, where ring closure only begins to occur following completion of the C–N bond rupture.

The retention of stereochemistry at the internal aziridine carbon C1 of 2b in 4ba provided an interesting clue to the exact nature of the rearrangement of INT2 to 4ba. One possibility involves intramolecular SN2 attack of the benzylic carbon on C1 of INT2 (Fig. 3a) to close the ring. However, it is unlikely a π orbital on the benzylic carbon can overlap effectively with the σ* of the aziridinium ylide to enable SN2. More importantly, SN2 attack at C1 would invert the stereochemistry at C1, which is ruled out by the X-ray crystal structure of 4cc. Rather, we propose ring expansion occurs via an unusual stereoretentive nucleophilic substitution; examples of this rare mechanism include the chlorination of secondary alcohols with thionyl chloride (SNi) and benzylic substitution in supramolecular cavities71,72. Computations also support a stereoretentive ‘SN1-like’ mechanism. First, the lower energy TS2′ is comprised mainly of C–N bond breakage at the external C1–N bond, which elongates to 1.937 Å. This contrasts to the bond-breaking sequence in the disfavored TS2, where the internal bicyclic C2–N bond shows more elongation at 2.360 Å, as compared with the C1–N bond (1.735 Å). Thus, even though both TS2 and TS2′ can be described as relatively low-barrier, early transition states, the extent to which the C–N bond breaks appears biased. Second, and consistent with our experimental results, TS2′ predicts that ring opening of the C1–N aziridine bond and the subsequent C–C bond formation must proceed with retention. This is largely due to the stereochemical relationship established between the nitrogen and the carbon substituents of the aziridine during formation of the aziridinium ylide; the carbamate tether controls this stereochemistry and likely plays a key role in restricting the conformational flexibility of the intermediates. Thus, the Me-bearing C1 of the original aziridine 2b is essentially a full carbocation, with nearly complete C–N rupture in TS2′ just prior to stereoretentive ring closure.

Chirality transfer

The synthetic utility of the sequential nitrene/carbene transfer reaction could be amplified by running it in tandem with asymmetric alkene aziridination to form enantioenriched piperidines over two steps. In 2017, we disclosed an asymmetric aziridination protocol using silver bisoxazoline complexes to enact intramolecular aziridination of homoallylic carbamate esters to achieve high enantioselectivities with a cost-effective catalyst67. Application of our asymmetric aziridination to the carbamate of (3Z)-3-penten-5-phenyl-1-ol gave enantioenriched (S,R)-2a67 in 95:5 er (Figs. 3b and 4a). Treatment of (S,R)-2a under the standard reaction conditions gives (R,R,R)-4aa with minimal loss of ee. Alternatively, a single catalyst could be used to accomplish the sequential nitrene/carbene transfer. Preparation of the allylic N-tosyloxycarbamate ester 5, according to the method described by Lebel et al.73, followed by treatment with Rh2(OAc)4 and K2CO3 generated the intermediate [5.3]-bicyclic aziridine. Filtration and solvent exchange, followed by slow addition of 3a, gave the unsaturated piperidine 4ja in 54% and >19:1 dr. Efforts to identify chemo- and enantioselective Rh-catalyzed aziridination catalysts are underway to secure access to these valuable heterocycles in one pot with good ee.

Fig. 4: Tandem nitrene/carbene chemistry and derivatization of products.

a Streamlining the nitrene/carbene transfer sequence. b Late-stage functionalization of complex molecules. c Further derivatization of dehydropiperidines.

Functionalization of dehydropiperidine products

Further functionalization of the unsaturated piperidine 4ca provides fully substituted, stereochemically rich piperidines in just two steps from the bicyclic aziridine. Indeed, treatment of 4ca with a higher order cuprate furnished 6 in 57% yield and >19:1 dr through a diastereoselective conjugate addition reaction; the relative stereochemistry was verified by both NOE and 1H NMR-coupling constants (Fig. 4b, see the Supplementary Information for details)74,75,76. In an initial effort to expand the conjugate addition reaction to include other heteroatom nucleophiles, we found treatment of 4ca with DBU and various nucleophiles did not furnish a conjugate addition product, but rather produced styrene 7 in 71% yield and excellent dr (Fig. 4b). Further treatment of 7 with mCPBA yielded epoxide 8 in equally good yield and dr. Furthermore, inspired by MacMillan and coworkers77, 4ca was found to undergo a radical Michael addition with Boc-protected tryptophan 9 to yield fully elaborated piperidine 10 in 32% yield as a single diastereomer. Although the yield was modest, this transformation rapidly builds complexity in two steps from a simple aziridine.

Finally, the mild reaction conditions and the transfer of the stereochemical information in the aziridine (S,R)-2a to (R,R,R)-4aa with good fidelity promoted us to explore the potential of this chemistry to append biomolecules to our unsaturated piperidine scaffolds. d-Phenylalanine and cholic acid were transformed into suitable diazoesters 11a and 11b (Fig. 4c), then subjected to treatment with (S,R)-2a (94% ee) under the standard reaction conditions. The products 12a and 12b were obtained in good yields, with excellent diastereoselectivities and enantiomeric ratios. We envisage this strategy could be effectively applied to explorations of new bioactive chemical space uncovered through fragment-based screening approaches.

In conclusion, aziridinium ylides, accessed in high diastereoselectivity from the intermolecular reaction of simple aziridines with metal-bound vinyl carbenes, are shown to be efficient intermediates for the conversion of small ring heterocycles to complex piperidines, a privileged motif in bioactive compounds. DFT computations, in tandem with transfer of chirality experiments, revealed that the ylides undergo a concerted, asynchronous, pseudo-[1,4]-sigmatropic rearrangement to yield products in high diastereoselectivity and with retention of ee installed in the aziridine precursor. Surprisingly, this chemistry bypasses deleterious cheletropic extrusion using unbiased aziridines to give synthetically useful yields of N-heterocycles. In addition, this mechanism proceeds with retention at the C–C bond, a unique consequence of the SN1-like closing of the vinyl anion tether. We anticipate this report will spur further research into the reactivity of both aziridinium ylides and other onium ylides derived from small-ring heterocycles.


General procedure for the dehydropiperidine synthesis

A flame-dried round bottom flask is placed under nitrogen and charged with Rh2(OAc)4 (0.03 equiv), followed by a solution of the aziridine (0.1 M in dry CH2Cl2). Upon the addition of the aziridine substrate, a color change of green to purple is observed. To this mixture is added a solution of the diazoester compound (1.2 equiv diluted to 0.1 M in CH2Cl2) dropwise over 2 h using a syringe pump. The conversion is checked by TLC and 1H NMR after the addition of the diazoester is complete; once all the starting material is consumed, the reaction mixture is concentrated and loaded directly onto a silica gel column for purification by chromatography using a gradient of 0–50% EtOAc/hexanes.

Data availability

The authors declare that all data supporting the findings of this study are available within the paper and its Supplementary Information and Supplementary Data 1 files, including experimental procedures, computational details, and characterization data for all new compounds. The crystallographic data for Compound 4cc are available in the CCDC repository under deposition number 1921208.


  1. 1.

    O’Hagan, D. Pyrrole, pyrrolidine, pyridine, piperidine and tropane alkaloids. Nat. Prod. Rep. 17, 435–446 (2000).

    Article  PubMed  Google Scholar 

  2. 2.

    Chen, Q., Gao, J., Zou, G. A., Xin, X. L. & Aisa, H. A. Piperidine alkaloids with diverse skeletons from Anacyclus pyrethrum. J. Nat. Prod. 81, 1474–1482 (2018).

    CAS  Article  PubMed  Google Scholar 

  3. 3.

    Vitaku, E., Smith, D. T. & Njardarson, J. T. Analysis of the structural diversity, substitution patterns, and frequency of nitrogen heterocycles among U.S. FDA-approved pharmaceuticals. J. Med. Chem. 57, 10257–10274 (2014).

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Taylor, R. D., MacCoss, M. & Lawson, A. D. Rings in drugs. J. Med. Chem. 57, 5845–5859 (2014).

    CAS  Article  PubMed  Google Scholar 

  5. 5.

    Baumann, M. & Baxendale, I. R. An overview of the synthetic routes to the best-selling drugs containing 6-membered heterocycles. Beilstein J. Org. Chem. 9, 2265–2319 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Lamberth, C. Heterocyclic chemistry in crop protection. Pest Manag. Sci. 69, 1106–1114 (2013).

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Nauen, R. & Jeshke, P. Neonicotinoids-from zero to hero in insecticide chemistry. Pest Manag. Sci. 64, 1084–1098 (2008).

    Article  CAS  PubMed  Google Scholar 

  8. 8.

    Lamberth, C. Pyrimidine chemistry in crop protection. Heterocycles 68, 561–603 (2006).

    CAS  Article  Google Scholar 

  9. 9.

    Lamberth, C. Pyrazole chemistry in crop protection. Heterocycles 71, 1467–1502 (2007).

    CAS  Article  Google Scholar 

  10. 10.

    Di, NisioM., Middeldorp, S. & Büller, H. R. Direct thrombin inhibitors. N. Engl. J. Med. 353, 1028–1040 (2005).

    Article  Google Scholar 

  11. 11.

    Ito, T., Otsuki, M. & Nishino, T. In vitro antibacterial activity of Q-35, a new fluoroquinolone. Antimicrob. Agents Chemother. 36, 1708–1714 (1992).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Brüning A. Analysis of Nelfinavir-Induced endoplasmic reticulum stress. In Methods in Enzymology. Academic Press 491, 127–142 (2011).

  13. 13.

    Børup, C., Meidahl, B., Petersen, I. M., Vangtorp, A. & le Fèvre Honoré, P. An early clinical Phase II evaluation of Paroxetine, a new potent and selective 5HT-uptake inhibitor in patients with depressive illness. Pharmacopsychiatry 15, 183–186 (1982).

    Article  Google Scholar 

  14. 14.

    Lund, J., Lomholt, B., Fabricius, J., Christensen, J. A. & Bechgaardl, E. Paroxetine: pharmacokinetics, tolerance and depletion of blood 5-HT in man. Acta Pharmacol. Toxicol. 44, 289–295 (1979).

    CAS  Article  Google Scholar 

  15. 15.

    Scannell, J. W., Blanckley, A., Boldon, H. & Warrington, B. Diagnosing the decline in pharmaceutical R&D efficiency. Nat. Rev. Drug Discov. 11, 191–200 (2012).

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Barker, A., Kettle, J., Nowak, T. & Pease, J. Expanding medicinal chemistry space. Drug Discov Today 18, 298–304 (2013).

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Lovering, F., Bikker, J. & Humblet, C. Escape from Flatland: Increasing saturation as an approach to improving clinical success. J. Med. Chem. 52, 6752–6756 (2009).

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Brown, D. G. & Bostrom, J. An analysis of past and present synthetic methodologies on medicinal chemistry: Where have all the new reactions gone? J. Med. Chem. 59, 4443–4458 (2016).

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Doak, B. C., Zheng, J., Dobrizsch, D. & Kihlberg, J. How beyond Rule of 5 drugs and clinical candidates bind to their targets. J. Med. Chem. 59, 2312–2327 (2016).

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Lovering, F. Escape from Flatland 2: complexity and promiscuity. Med. Chem. Commun. 4, 515–519 (2013).

    CAS  Article  Google Scholar 

  21. 21.

    Ma, C. et al. 1-Azadienes as regio- and chemoselective dienophiles in aminocatalytic asymmetric Diels–Alder reaction. Org. Lett. 15, 6206–6209 (2013).

    CAS  Article  PubMed  Google Scholar 

  22. 22.

    Jarrige, L., Blanchard, F. & Masson, G. Enantioselective organocatalytic intramolecular aza-Diels–Alder reaction. Angew. Chem. Int. Ed. 56, 10573–10576 (2017).

    CAS  Article  Google Scholar 

  23. 23.

    Wang, H., Zhou, W., Tao, M., Hu, A. & Zhang, J. Functionalized tetrahydropyridines by enantioselective phosphine-catalyzed aza-[4+2] cycloaddition of N-sulfonyl-1-aza-1,3-dienes with vinyl ketones. Org. Lett. 19, 1710–1713 (2017).

    CAS  Article  PubMed  Google Scholar 

  24. 24.

    He, M., Struble, J. R. & Bode, J. W. Highly enantioselective azadiene Diels–Alder reactions catalyzed by chiral N-heterocyclic carbenes. J. Am. Chem. Soc. 128, 8418–8420 (2006).

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Chu, J. C., Dalton, D. M. & Rovis, T. Zn-catalyzed enantio- and diastereoselective formal [4+2] cycloaddition involving two electron-deficient partners: Asymmetric synthesis of piperidines from 1-azadienes and nitro-alkenes. J. Am. Chem. Soc. 137, 4445–4452 (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Bossharth, E., Desbordes, P., Monteiro, N. & Balme, G. Palladium-mediated three-component synthesis of furo[2,3-b]pyridones by one-pot coupling of 3-iodopyridones, alkynes, and organic halides. Org. Lett. 5, 2441–2444 (2003).

    CAS  Article  PubMed  Google Scholar 

  27. 27.

    Shu, C. & Liebeskind, L. S. Enantiocontrolled synthesis of 2,6-disubstituted piperidines by desymmetrization of meso-η-(3,4,5)-dihydropyridinylmolybdenum complexes. Application to the total synthesis of (−)-dihydropinidine and (−)-andrachcinidine. J. Am. Chem. Soc. 125, 2878–2879 (2003).

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Fayol, A. & Zhu, J. Synthesis of polysubstituted 4,5,6,7-tetrahydrofuro[2,3-c]pyridines by a novel multicomponent reaction. Org. Lett. 6, 115–118 (2004).

    CAS  Article  PubMed  Google Scholar 

  29. 29.

    Zhu, W. et al. Multicomponent reactions for the synthesis of complex piperidine scaffolds. Angew. Chem. Int. Ed. 48, 5880–5883 (2009).

    CAS  Article  Google Scholar 

  30. 30.

    Jakubec, P., Cockfield, D. M., Helliwell, M., Raftery, J. & Dixon, D. J. Stereoselective, nitro-Mannich/lactamization cascades for the direct synthesis of heavily decorated 5-nitropiperidin-2-ones and related heterocycles. Beilstein J. Org. Chem. 8, 567–578 (2012).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  31. 31.

    Kubota, K., Watanabe, Y., Hayama, K. & Ito, H. Enantioselective synthesis of chiral piperidines via the stepwise dearomatization/borylation of pyridines. J. Am. Chem. Soc. 138, 4338–4341 (2016).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  32. 32.

    Katsuki, T. & Martin, V. Asymmetric epoxidation of allylic alcohols: the Katsuki–Sharpless epoxidation reaction. Org. React. 48, 1–299 (2004).

    Google Scholar 

  33. 33.

    Zhang, W., Loebach, J. L., Wilson, S. R. & Jacobsen, E. N. Enantioselective epoxidation of unfunctionalized olefins catalyzed by salen manganese complexes. J. Am. Chem. Soc. 112, 2801–2803 (1990).

    CAS  Article  Google Scholar 

  34. 34.

    Katsuki, T. & Sharpless, K. B. The first practical method for asymmetric epoxidation. J. Am. Chem. Soc. 102, 5974–5976 (1980).

    CAS  Article  Google Scholar 

  35. 35.

    Li, Z., Conser, K. R. & Jacobsen, E. N. Asymmetric alkene aziridination with readily available chiral diimine-based catalysts. J. Am. Chem. Soc. 115, 5326–5327 (1993).

    CAS  Article  Google Scholar 

  36. 36.

    Evans, D. A., Faul, M. M., Bilodeau, M. T., Anderson, B. A. & Barnes, D. M. Bis (oxazoline)-copper complexes as chiral catalysts for the enantioselective aziridination of olefins. J. Am. Chem. Soc. 115, 5328–5329 (1993).

    CAS  Article  Google Scholar 

  37. 37.

    Ghorai, M. K., Bhattacharyya, A., Das, S. & Chauhan, N. Ring expansions of activated aziridines and azetidines in Synthesis of 4-to 7-membered heterocycles by ring expansion. Springer, 49–142, (2015).

  38. 38.

    Ghorai, M. K., Shukla, D. & Bhattacharyya, A. Syntheses of chiral β-and γ-amino ethers, morpholines, and their homologues via nucleophilic ring-opening of chiral activated aziridines and azetidines. J. Org. Chem. 77, 3740–3753 (2012).

    CAS  Article  PubMed  Google Scholar 

  39. 39.

    Schomaker, J. M., Bhattacharjee, S., Yan, J. & Borhan, B. Diastereomerically and enantiomerically pure 2,3-disubstituted pyrrolidines from 2,3-aziridin-1-ols using a sulfoxonium ylide: a one-carbon homologative relay ring expansion. J. Am. Chem. Soc. 129, 1996–2003 (2007).

    CAS  Article  PubMed  Google Scholar 

  40. 40.

    Ilardi, E. A. & Njardarson, J. T. Ring expansions of vinyloxiranes, -thiiranes, and-aziridines: synthetic approaches, challenges, and catalytic success stories. J. Org. Chem. 78, 9533–9540 (2013).

    CAS  Article  PubMed  Google Scholar 

  41. 41.

    Brichacek, M., Navarro Villalobos, M., Plichta, A. & Njardarson, J. T. Stereospecific ring expansion of chiral vinyl aziridines. Org. Lett. 13, 1110–1113 (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  42. 42.

    Mack, D. J. & Njardarson, J. T. Recent advances in the metal-catalyzed ring expansions of three-and four-membered rings. ACS Catal. 3, 272–286 (2013).

    CAS  Article  Google Scholar 

  43. 43.

    D’hooghe, M. & Ha, H. J. Synthesis of 4-to 7-membered heterocycles by ring expansion. in Topics in Heterocyclic Chemistry. Springer 41 (2016).

  44. 44.

    Provoost, O. Y., Hazelwood, A. J. & Harrity, J. P. Pd-catalysed [3+3] annelations in the stereoselective synthesis of indolizidines. Beilstein J. Org. Chem. 3, (2007).

  45. 45.

    Piens, N. & D’hooghe, M. Carbonylation of aziridines as a powerful tool for the synthesis of functionalized β-lactams. Eur. J. Org. Chem. 40, 5943–5960 (2017).

  46. 46.

    Feng, J.-J., Lin, T.-Y., Wu, H.-H. & Zhang, J. Modular access to the stereoisomers of fused bicyclic azepines: Rhodium-catalyzed intramolecular stereospecific hetero-[5+2] cycloaddition of vinyl aziridines and alkenes. Angew. Chem. Int. Ed. 54, 15854–15858 (2015).

    CAS  Article  Google Scholar 

  47. 47.

    Aahman, J. & Somfai, P. Aza-[2,3]-Wittig rearrangements of vinylaziridines. J. Am. Chem. Soc. 116, 9781–9782 (1994).

    CAS  Article  Google Scholar 

  48. 48.

    Clark, J. S. et al. Rearrangement of ammonium ylides produced by intramolecular reaction of catalytically generated metal carbenoids. Part 2. Stereoselective synthesis of bicyclic amines. J. Chem. Soc. Perkin Trans. 1, 3325 (2001).

    Google Scholar 

  49. 49.

    Kentish Barnes, W. & Rowlands, G. J. Studies on the [2,3]-Stevens rearrangement of aziridinium ions. Tetrahedron Lett. 45, 5347–5350 (2004).

    Article  CAS  Google Scholar 

  50. 50.

    Hata, Y. & Watanabe, M. Fragmentation reaction of aziridinium ylides. Tetrahedron Lett. 46, 4659–4660 (1972).

    Article  Google Scholar 

  51. 51.

    Dolfen, J., Kenis, S., Van Hecke, K., De Kimpe, N. & D’hooghe, M. Selective synthesis of functionalized trifluoromethylated pyrrolidines, piperidines, and azepanes starting from 1‐tosyl‐2‐(trifluoromethyl) aziridine. Chem. Eur. J. 20, 10650–10653 (2014).

    CAS  Article  PubMed  Google Scholar 

  52. 52.

    Coldham, I., Collis, A. J., Mould, R. J. & Rathmell, R. E. Ring expansion of aziridines to piperidines using the aza-Wittig rearrangement. Tetrahedron Lett. 36, 3557–3560 (1995).

    CAS  Article  Google Scholar 

  53. 53.

    Vervisch, K., D’hooghe, M., Törnroos, K. W. & De Kimpe, N. Synthesis of stereodefined piperidines from aziridines and their transformation into conformationally constrained amino acids, amino alcohols and 2,7-diazabicyclo[3.3.1]nonanes. J. Org. Chem. 75, 7734–7744 (2010).

    CAS  Article  PubMed  Google Scholar 

  54. 54.

    Jarvis, S. B. & Charette, A. B. Synthesis of enantiopure substituted piperidines via an aziridinium ring expansion. Org. Lett. 13, 3830–3833 (2011).

    CAS  Article  PubMed  Google Scholar 

  55. 55.

    Boralsky, L. A., Marston, D., Grigg, R. D., Hershberger, J. C. & Schomaker, J. M. Allene functionalization via bicyclic methyleneaziridines. Org. Lett. 13, 1924–1927 (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  56. 56.

    Rigoli, J. W. et al. 1,4-Diazaspiro[2.2]pentanes as a flexible platform for the synthesis of diamine-bearing stereotriads. J. Org. Chem. 77, 2446–2455 (2012).

    CAS  Article  ADS  PubMed  PubMed Central  Google Scholar 

  57. 57.

    Adams, C. S., Boralsky, L. A., Guzei, I. A. & Schomaker, J. M. Modular functionalization of allenes to aminated stereotriads. J. Am. Chem. Soc. 134, 10807–10810 (2012).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  58. 58.

    Adams, C. S., Grigg, R. D. & Schomaker, J. M. Complete stereodivergence in the synthesis of 2-amino-1,3-diols from allenes. Chem. Sci. 5, 3046–3056 (2014).

    CAS  Article  Google Scholar 

  59. 59.

    Burke, E. G. & Schomaker, J. M. Oxidative allene amination for the synthesis of azetidin-3-ones. Angew. Chem. Int. Ed. 54, 12097–12101 (2015).

    CAS  Article  Google Scholar 

  60. 60.

    Gerstner, N. C., Adams, C. S., Tretbar, M. & Schomaker, J. M. Stereocontrolled syntheses of seven-membered carbocycles by tandem allene aziridination/[4+3] reaction. Angew. Chem. Int. Ed. 128, 13434–13437 (2016).

    Article  Google Scholar 

  61. 61.

    Adams, C. S., Weatherly, C. D., Burke, E. G. & Schomaker, J. M. The conversion of allenes to strained three-membered heterocycles. Chem. Soc. Rev. 43, 3136–3316 (2014).

    CAS  Article  PubMed  Google Scholar 

  62. 62.

    Schmid, S. C., Guzei, I. A. & Schomaker, J. M. A stereoselective [3+1] ring expansion for the synthesis of highly substituted methylene azetidines. Angew. Chem. Int. Ed. 56, 12229–12233 (2017).

    CAS  Article  Google Scholar 

  63. 63.

    Schmid, S. C., Guzei, I., Fernandez, I. & Schomaker, J. M. Ring expansion of bicyclic methyleneaziridines via concerted, near-barrierless [2,3]-Stevens rearrangement. ACS Catal. 8, 7907–7914 (2018).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  64. 64.

    Davies, H. M. & Morton, D. Guiding principles for site selective and stereoselective intermolecular C–H functionalization by donor/acceptor rhodium carbenes. Chem. Soc. Rev. 40, 1857–1869 (2011).

    CAS  Article  PubMed  Google Scholar 

  65. 65.

    Ahmend, G., Churchhill, M. R. & Davies, H. L. M. Asymmetric synthesis of highly functionalized 8-oxabicyclo[3.2.1]octene derivatives. J. Am. Chem. Soc. 118, 10774–10782 (1996).

    Article  Google Scholar 

  66. 66.

    Yang, J., Manning, J. R. & Davies, H. M. L. Direct synthesis of methyl 2-diazo-4-aryl-3-butenoates and their application to the enantioselective synthesis of 4-aryl-4-(1-naphthyl)-2-butenoates. Tetrahedron. Asymm. 17, 665–673 (2006).

    Article  CAS  Google Scholar 

  67. 67.

    Ju, M., Weatherly, C. D., Guzei, I. A. & Schomaker, J. M. Chemo- and enantioselective intramolecular silver-catalyzed aziridinations. Angew. Chem. Int. Ed. 56, 9944–9948 (2017).

    CAS  Article  Google Scholar 

  68. 68.

    Bhakat, S. The controversial reaction mechanism of Stevens rearrangement: a review. J. Chem. Pharm. Res. 3, 115–121 (2011).

    CAS  Google Scholar 

  69. 69.

    Sweeney, J. B. Sigmatropic rearrangements of ‘ononium’ ylids. Chem. Soc. Rev. 38, 1027–1038 (2009).

    CAS  Article  PubMed  Google Scholar 

  70. 70.

    Adrojewski, T. & Jonczyk, A. On the [1,4] sigmatropic rearrangement of ammonium benzylides. Tetrahedron Lett. 36, 1355–1358 (1995).

    Article  Google Scholar 

  71. 71.

    Müller, P. & Rossier, J.-C. Solvent effects on the steric course of the solvolysis of tertiary acyclic derivatives. J. Chem. Soc. Perk. Trans. 2, 2232–2239 (2000).

    Article  Google Scholar 

  72. 72.

    Zhao, C., Toste, F. D., Raymond, K. N. & Bergman, R. G. Nucleophilic substitution catalyzed by a supramolecular cavity proceeds with retention of absolute stereochemistry. J. Am. Chem. Soc. 136, 14409–14412 (2014).

    CAS  Article  PubMed  Google Scholar 

  73. 73.

    Lebel, H., Lectard, S. & Parmentier, M. Copper-catalyzed alkene aziridination with N-tosyloxycarbamates. Org. Lett. 9, 4797–4800 (2007).

    CAS  Article  PubMed  Google Scholar 

  74. 74.

    Toyooka, N. et al. Synthesis of alkaloid 223A and a structural revision. Org. Lett. 4, 1715–1717 (2002).

    CAS  Article  PubMed  Google Scholar 

  75. 75.

    Okada, T. et al. Synthesis of 8-deoxypumiliotoxin 193H and 9-deoxyhomopumiliotoxin 2070. Tetrahedron Lett. 59, 3797–3800 (2018).

    CAS  Article  Google Scholar 

  76. 76.

    Toyooka, N., Fukutome, A., Shinoda, H. & Nemoto, H. Stereodivergent synthesis of the 2,3,5,6-tetrasubstituted piperidine ring system: An application to the synthesis of alkaloids 223A and 205B from poison frogs. Tetrahedron 60, 6197–6216 (2004).

    CAS  Article  Google Scholar 

  77. 77.

    Chu, L., Ohta, C., Zuo, Z. & MacMillan, D. W. C. Carboxylic acids as a traceless activation group for conjugate additions: a three-step synthesis of (±)-pregabalin. J. Am. Chem. Soc. 136, 10886–10889 (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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J.M.S. thanks the NIH 1R01GM132300-01 and the University of Wisconsin Vilas Faculty Mid-Career Award for support of this work. The NMR facilities at UW-Madison are funded by the National Science Foundation (NSF; CHE-9208463, CHE-9629688) and National Institutes of Health (NIH; RR08389-01). The Q-Exactive mass spectrometer was acquired from an NIH-S10 award (NIH-1S10OD020022-1). I.F. acknowledges financial support from the Spanish MINECO-FEDER (Grants CTQ2016-78205-P and CTQ2016-81797-REDC).

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J.M.S., K.A.N., J.E., and S.C.S designed the research. J.E. and K.A.N. synthesized substrates, developed the reaction conditions, carried out scope studies and post-functionalizations of the dehydropiperidine products. W.T.R. synthesized substrates. I.A.G. carried out the single X-ray crystal structure studies. I.F. carried out the computational studies. J.M.S., S.C.S., J.E., K.A.N., and I.F. co-wrote the paper. All the authors discussed the results and commented on the paper.

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Correspondence to Jennifer M. Schomaker.

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Eshon, J., Nicastri, K.A., Schmid, S.C. et al. Intermolecular [3+3] ring expansion of aziridines to dehydropiperi-dines through the intermediacy of aziridinium ylides. Nat Commun 11, 1273 (2020).

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