A major challenge in the treatment of retinal degenerative diseases, with the transplantation of replacement photoreceptors, is the difficulty in inducing the grafted cells to grow and maintain light sensitive outer segments in the host retina, which depends on proper interaction with the underlying retinal pigment epithelium (RPE). Here, for an RPE-independent treatment approach, we introduce a hyperpolarizing microbial opsin into photoreceptor precursors from newborn mice, and transplant them into blind mice lacking the photoreceptor layer. These optogenetically-transformed photoreceptors are light responsive and their transplantation leads to the recovery of visual function, as shown by ganglion cell recordings and behavioral tests. Subsequently, we generate cone photoreceptors from human induced pluripotent stem cells, expressing the chloride pump Jaws. After transplantation into blind mice, we observe light-driven responses at the photoreceptor and ganglion cell levels. These results demonstrate that structural and functional retinal repair is possible by combining stem cell therapy and optogenetics.
Cell replacement therapy offers hope for the treatment of late-stage retinal degeneration, when the outer retinal photoreceptor layer is lost1,2,3. However, a remaining obstacle of photoreceptor replacement is that transplanted cells have to develop into functional photoreceptors with light sensitive outer segments (OS). Indeed, in mouse models of severe degeneration, the formation of light-sensitive OS by transplanted photoreceptors has been difficult to achieve4,5,6. Recent studies, using retinal sheet transplantation lead to major improvements in terms of OS formation and light sensitivity7,8. Despite these promising results, a major problem has not yet been solved: photoreceptors need tight interaction with the retinal pigment epithelium (RPE) in order to maintain their structure and function via continuous disc shedding and renewal9. Since in retinal degenerative diseases the RPE is often also compromised9,10, the probability that transplanted photoreceptors stay sensitive to light is very low11,12. To tackle this problem, we introduce optogenetic light sensors into photoreceptors, derived from the developing mouse retina as well as from human induced pluripotent stem cells (hiPSCs), and transplant them into mouse models of severe retinal degeneration. The key point of our approach is that these optogenetically-transformed photoreceptors stay functional based on the activity of the microbial opsin, even in the absence of properly formed OS and without the support from the RPE.
Neonatal mouse-derived NpHR photoreceptor precursors
For optogenetic transformation of mouse photoreceptors, eyes of newborn wild-type mice at post-natal day (P) 2 were injected with an adeno-associated viral (AAV) vector encoding enhanced Natronomonas pharaonis halorhodopsin eNpHR2.0 (NpHR)13 under the control of the rhodopsin promoter (AAV-Rho-NpHR-YFP) (Fig. 1a and Supplementary Fig. 1). At P4, photoreceptor precursors were sorted by magnetic activated cell sorting (MACS) using the photoreceptor specific cell surface marker CD7314,15. The harvested cells were transplanted via sub-retinal injections into two blind mouse models of late-stage retinal degeneration (Cpfl1/Rho−/− mice16 aged 9 to 18 weeks and C3H rd/rd (rd1) mice17 aged 4 to 11 weeks; see Supplementary Table 1 for a complete overview of mouse ages). At these ages, the vast majority of outer nuclear layer (ONL) cells were lost in host mice (Fig. 1b, e). Cpfl1/Rho−/− mice are left with 2–3 rows of photoreceptors at the age of 9 weeks, and a single row of photoreceptors by 10–12 weeks of age. These mice are born with non-functional rods and cones16. Rd1 mice loose photoreceptor OS and only a single row of cone cell bodies in the ONL remains by 3 weeks after birth18. Four weeks after transplantation, we investigated the morphology of the transplanted donor cells and their ability to integrate into the host retina. In both mouse models, we found NpHR-positive donor cells in close contact to cell bodies of rod bipolar cells, but none of the transplanted cells displayed correctly formed OS (Fig. 1c, d, f, g). Transplanted cells expressed the synaptic marker Synaptophysin (Supplementary Fig. 2) suggesting synapse formation between donor photoreceptors and the downstream neurons. We quantified the number of YFP+ cells in the subretinal space transplanted with donor-derived NpHR-expressing rod precursors and found substantial numbers of cells to survive at four weeks post transplantation (Supplementary Fig. 3). Next, we assessed potential material transfer between transplanted cells and remaining photoreceptors by fluorescence in situ hybridization with Y chromosome-specific probe (Y chromosome FISH). NpHR-expressing rod precursors derived from male P4 mice were injected into female Cpfl1/Rho−/− mice at 9 weeks of age, and imaged after 4 weeks using structured illumination microscopy (Fig. 1h). Y chromosome+/YPF+ cells (transplanted donor cells) and Y chromosome−/YFP+ cells (endogenous photoreceptor that underwent material transfer) were quantified. 90% of YFP+ cells co-stained with Y chromosome probe, leaving only very few cells exclusively YFP+. This could either be due to an artifact or very rare events of cytoplasmic exchange among donor and host photoreceptors (Fig. 1h, i). We then tested if we can elicit light responses from these NpHR-positive donor cells in the absence of functional OS. Two-photon targeted patch-clamp recordings revealed robust responses to orange light pulses (580 nm, 1016 photons cm−2s−1) (Fig. 2b and Supplementary Fig. 4). There were no measurable light-evoked currents in transplanted photoreceptors expressing GFP only, which is consistent with the finding that the transplanted cells lacked their light sensitive OS. Stimulation at different wavelengths showed a spectral sensitivity matching the action spectrum of NpHR (Fig. 2b). To measure the temporal properties of NpHR-positive photoreceptors, we recorded photocurrents using light pulses at increasing frequencies, and we observed that they could follow up to 25 Hz (Fig. 2c and Supplementary Fig. 4). Although, frequencies above 10 Hz are filtered out by the bipolar cells, the ability of optogenetically engineered photoreceptors to respond to light in a faster than natural pace implies that retinal ganglion cells (RGCs) receiving signal from these cells should follow high-frequency stimulation in a similar manner to normal retina19. The rise constants were significantly faster compared to photocurrents of wild-type mice (Fig. 2d). Both, from the spectral (peak current at 580 nm) and the temporal (TauON < 10 ms) response properties we concluded that the photocurrents were driven by the introduced NpHR (Fig. 2a–d and Supplementary Fig. 4).
Connectivity and signal transmission to host neurons
Next, we investigated if the signals from transplanted photoreceptors are transmitted to RGCs, the output neurons of the retina. By using extracellular spike recordings, we measured ON- and OFF-light responses in RGCs. These results demonstrate that NpHR-induced signals are transmitted to the retinal output neurons via ON- and OFF-pathways suggesting that the transplanted photoreceptors can form functional synaptic connections with the inner retinal neurons (Fig. 3a and Supplementary Fig. 5), which was supported by histological analysis (Supplementary Fig. 2). Recordings performed under pharmacological block of photoreceptor input to ON-bipolar cells (50 µM L-AP4) showed complete abolition of ON light responses, which recovered after 20 min of L-AP4-washout. These control experiments confirmed that light-induced signals were indeed transmitted via photoreceptor-to-bipolar cell synapses (Fig. 3b, c). By stimulating treated retinas at different wavelengths we determined the spectral sensitivity of the light responses, which peaked at 580–600 nm, reflecting the action spectrum of NpHR (Fig. 3d, e). To assess the light intensities required to trigger spike responses, we used light pulses (580 nm) at different intensities. Importantly, the intensities required to evoke light responses were well below the safety limit for optical radiation in the human eye20,21 (Fig. 3f, g and Supplementary Fig. 5). We did not observe measurable light responses in retinae from age-matched control-mice, where photoreceptor precursors expressing only GFP were transplanted (Fig. 3h, i and Supplementary Fig. 5). Lastly, to test whether the behaviour of treated mice could be modulated by light, we used the light/dark box test22 employing high intensity orange light (Fig. 3j). Treated Cpfl1/Rho−/− mice displayed robust light avoidance behaviour (40.7 ± 3.5% of time in the illuminated compartment), compared to non-injected (59.8 ± 2.2%) mice and mice transplanted with photoreceptor precursors expressing GFP (56.6 ± 4.5%) (Fig. 3k).
Generation of hiPSC-derived Jaws-expressing photoreceptors
To evaluate the translatability of our approach to human subjects, we asked if it is possible to replace the mouse donor cells with optogenetically-transformed hiPSCs (Fig. 4a). To do so, we first optimized a previous protocol of differentiation based on the self-generation of 3D neural-retina-like structures23. Using this system, we generated cone-enriched retinal organoids, expressing the pan-photoreceptor markers Cone Rod Homeobox (CRX) and recoverin (RCVRN) alongside the cone-specific marker cone arrestin (CAR) (Fig. 4b–f and Supplementary Fig. 6). Contrary to nocturnal rodents, cone photoreceptors are responsible for high acuity daylight vision in humans, and are therefore the preferred choice for transplantation. To render these immature cones light sensitive, we used the hyperpolarizing chloride pump Jaws, a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum and engineered to result in red light–induced photocurrents three times those of earlier silencers24. Jaws was chosen for iPSC experiments based on its enhanced expression level and improved membrane trafficking in human tissue, compared to NpHR24,25,26. By using an AAV vector, encoding Jaws-GFP under the control of CAR promoter, we delivered the microbial opsin to the hiPSC-derived cone photoreceptors (Fig. 4g, h). Single cell recordings from optogenetically transformed cones in retinal organoids revealed solid light responses, matching the response properties of Jaws, while recordings from hiPSC-derived cones, expressing GFP only, showed no light responses (Fig. 4i–l). Additionally, monolayer cultures of these human cones expressing Jaws, maintained their ability to strongly respond to light after dissociation of the retinal organoids (Supplementary Fig. 7). These results collectively demonstrate the possibility to induce robust optogenetic light responses in photoreceptors derived from hiPSCs in the absence of light sensitive OS.
Transplantation of hiPSC-derived Jaws-expressing PRs
In order to transplant Jaws-positive photoreceptors, we dissociated the retinal organoids and injected the cell suspension subretinally into the blind hosts (Cpfl1/Rho−/−, age 10 to 15 weeks; rd1, age 4 to 5 weeks). In both Cpfl1/Rho−/− and rd1 mice, we observed Jaws-expressing donor cells in close proximity to the host INL several weeks after transplantation (Fig. 5a–c). Due to recent concerns about material transfer in photoreceptor transplantation27,28,29, we stained cryosections from the transplanted retinas with the human nuclear antigen (HNA) and we examined the size of the transplanted cells (HNA+) in relation to the chromatin structure and diameter of host cells (Fig. 5d). HNA stained cell counts confirmed that only a very small portion (5%) of the GFP+ labeled cells could potentially be endogenous mouse cells that underwent material transfer (HNA−/GFP+) (Supplementary Fig. 8). Both the HNA staining and nuclei comparison confirmed the human identity of transplanted cells in close proximity of the host INL. The transplanted GFP+ cells were RCVRN positive (Supplementary Fig. 8) and located next to PKCα-positive bipolar cells (Fig. 5a). They expressed the synaptic marker Synaptophysin in close apposition to the bipolar cell dendrites (Fig. 5b), suggesting that the human cells form synaptic connections with the host bipolar cells. The transplanted cells displayed robust Jaws-induced photocurrents by patch clamp, demonstrating the functionality of the microbial opsin in the host environment (Fig. 5e). The measured photocurrents peaked at 575 nm and showed fast kinetics (TauON < 10 ms) (Fig. 5f–h), reflecting the response properties of Jaws. At the ganglion cell level, we observed ON- and OFF responses from different ganglion cell types, which shows that Jaws-driven signals from transplanted photoreceptors were transmitted via second order neurons (Supplementary Fig. 9) to ON and OFF ganglion cells (Fig. 5i and Supplementary Fig. 10). The light intensity requirements were again below the safety threshold for the human retina20,21 (Fig. 5j and Supplementary Fig. 10). After transplantation of control human donor cells, expressing GFP only, no light responses were detected (Fig. 4k and Supplementary Fig. 10), as expected in absence of OS-like structures.
Transplantation of healthy photoreceptors holds great promise to restore vision in patients with outer retinal degeneration. This approach has received significant attention over the past years as it can restore vision independently from the cause of photoreceptor cell loss30. Significant progress has been made in the generation23,26,31,32,33,34,35, purification36,37 and transplantation of photoreceptors3,7,35,36,38,39,40 from hiPSCs. However, photoreceptor replacement faces a three-fold challenge: transplanted cells need to develop (1) synaptic contact to bipolar cells for signal transmission, (2) functional photoreceptor OS, and (3) tight contact to RPE cells to maintain OS light-sensitivity (Fig. 6). This makes photoreceptor transplantation complex and challenging. Recent studies have shown that the recipient environment is of great importance for successful integration and survival of transplanted photoreceptor cells. In animals with severely degenerated ONL, transplanted photoreceptor precursors derived from postnatal mouse retina4,5,6 or from hiPSCs35 failed to develop normal OS structure and establish correct OS polarity with respect to host RPE. The RPE cells are indispensable for OS renewal as they phagocytose the shed OS discs. Moreover, they re-isomerize the chromophore all-trans-retinal into 11-cis-retinal. Thus, in the absence of intimate contact with the RPE photoreceptors cannot maintain their light sensitivity41.
For an OS and RPE-independent treatment approach, we introduced a hyperpolarizing microbial opsin into photoreceptors derived from either neo-natal mouse retinas or from human retinal organoids derived from iPSCs. We transplanted these optogenetically-transformed photoreceptors into blind mice lacking the photoreceptor layer. We have shown that these cells can mediate visual function, as demonstrated by a battery of tests from RGC recordings to behavioural tests. The paradigm that transplanted photoreceptors migrate and structurally integrate into the ONL of the recipient has been challenged recently by several groups27,28,29 providing strong evidence that cytoplasmic material transfer occurs between transplanted cells, residing in the sub-retinal space, and remaining photoreceptor cells of the host. In these experiments, however, late-stage degeneration animals were used to model patients with advanced disease, thus there are only few remaining photoreceptors, minimizing the potential contribution of material transfer42. To distinguish between potential fusion events and structural integration of donor photoreceptors, we performed Y chromosome FISH and HNA staining in the Cpfl1/Rho−/− model where some remaining cells were visible in earlier transplantation time-points. Our Y chromosome FISH experiments revealed a very limited number of events of potential material transfer (<10%). In our blind rd1 mice, only sparse population of cones and no rods remain after 36 days of age18. We confirmed this observation in our control animals, obviating the possibility of material transfer from the transplanted NpHR-expressing mouse progenitors to remnant ONL cells of the host. Moreover, NpHR-positive cells that were attached to the host INL visibly show rod nuclear morphology, indicating that these are indeed donor cells and not remaining cones. As for the transplantation of Jaws-expressing hiPSCs, histological analysis using a human-specific nuclear marker (HNA) in transplanted mice, confirmed that the vast majority (95%) of GFP-expressing cells were HNA positive. This result along with the measured nuclei size confirmed the human origin of the transplanted cells, ruling out material exchange between human donor photoreceptors and mouse host cells. Although these do not fully rule out that material transfer may contribute to the improved functional responses, we have observed that the level of functional improvement is independent of the host age at time of transplantation, further supporting the optogenetically transformed photoreceptors are the major source of functional light responses (Supplementary Fig. 11). Moreover, material transfer is rare between human donor and mouse host photoreceptors35,36 (Fig. 5d and Supplementary Fig. 8), arguing against a significant contribution of material transfer to the observed functional improvements.
Lastly, any possible rescue effect mediated by remaining host photoreceptors is expected to be very minor as our control groups transplanted at the same ages with wild-type donor-derived photoreceptor precursors or hiPSC-derived photoreceptors expressing GFP only, never showed any detectable functional responses. This confirms that any possible rescue effect on remaining host photoreceptors cannot be a result of the transplantation itself and suggests that the functional outcomes are a direct consequence of the presence of an optogenetic protein expressed in the transplanted photoreceptors.
In conclusion, by using immature photoreceptors equipped with a microbial opsin, we went beyond the current limitations of optogenetic gene therapy approaches. Optogenetic approaches commonly target bipolar cells or RGCs that are viable targets in late stages of retinal degenerative diseases such as retinitis pigmentosa or age-related macular degeneration. Unfortunately, conferring light sensitivity to cells downstream from photoreceptors, bypasses the important information processing normally conducted by the inner retinal circuitry. Photoreceptor-directed optogenetic therapy that aims to rescue the function of remaining dormant cones harnesses the information processing of the inner retina allowing the recovery of complex visual responses such as lateral inhibition and directional selectivity in previously blind mice43, but this strategy can only be useful in patients with remaining cones which represent a minor portion of late-stage retinitis pigmentosa patients44. Here, we use the synergy of cell replacement and optogenetic therapy that allows the restoration of retinal structure with stem cell derivatives and visual function with microbial opsins. In a future perspective, optogenetically engineered hiPSC-derived cones could serve as donor cells for photoreceptor transplantation in late-stage retinal degeneration. In patients, degenerative diseases of the retina such as retinitis pigmentosa, age-related macular degeneration, and Leber congenital amaurosis, often manifest RPE degeneration along with photoreceptor degeneration, especially in their late stages10,45,46,47. Our approach bodes well for applications in such patients who can only obtain limited benefit from transplantation of photoreceptors in the absence of chromophore replenishment from their dystrophic RPE.
Wild-type C57BL/6 mice (Janvier Laboratories) were used as a source of photoreceptor precursor donor cells. The following two models are both models of late-stage degeneration and were used as cell recipients. Cone photoreceptor function loss 1/rhodopsin-deficient double-mutant Cpfl1/Rho−/− mice16 were provided by Marius Ader and rederived by Charles River Laboratory. The line was the result of crossing Cone photoreceptor function loss 1 (Cpfl1) mice48 with rhodopsin knock-out mice (Rho−/−)49. The outcome were mice with no functional photoreceptors starting from eye opening and with the ONL degenerating to one row of cell bodies by 10 to 12 weeks16. Retinal degeneration 1 (rd1) mice (C3Hrd/rd)17 were provided by Thierry Leveillard. The retina in these mice degenerates to a single row of cones by 3 weeks of age18,50.
All mice were housed under a 12-h light-dark cycle with free access to food and water. We have complied with all relevant ethical regulations for animal testing and research. All animal experiments and procedures were approved by the local animal experimentation ethics committee (Le Comité d’Ethique pour l’Expérimentation Animale Charles Darwin) and were carried out according to institutional guidelines in adherence with the National Institutes of Health guide for the care and use of laboratory animals as well as the Directive 2010/63/EU of the European Parliament.
Recombinant AAVs were produced using the triple-transfection method on HEK293 cells (ATCC CRL-1573), harvested 24–72 h post transfection and purified by iodixanol gradient ultracentrifugation51. The 40% iodixanol fraction was collected after a 90 min spin at 354000 g. Concentration and buffer exchange were performed against PBS containing 0.001% Pluronic. AAV vector stocks titers were then determined based on real-time quantitative PCR titration method using ITR primers52 and SYBR Green (Thermo Fischer Scientific).
AAV-infection of photoreceptor precursors
Wild-type mice (C57BL/6J) at P2 were anesthetized on ice. Eyelids were cut and 1 µl of AAV9 2YF carrying eNpHR gene under the control of human rhodopsin promoter and fused to the fluorescent reporter eYFP (AAV9 2YF hRho-eNpHR-eYFP), or of AAV9 2YF hRho-GFP in the case of GFP only-expressing controls, was injected bilaterally using an ultrafine 34-gauge Hamilton syringe.
MACS with CD73
Two days following the AAV injections in P2 mice, at P4, retinas were isolated from the injected wild-type mice and cells were enriched using CD73 cell surface marker before transplantation14,15. Briefly, retinas were dissociated, pelleted by centrifugation (5 min at 300 g), resuspended in 500 µL MACS buffer (phosphate-buffered saline [PBS; pH 7.2], 0.5% BSA, 2 mM EDTA) and incubated with 10 µg/mL rat anti-mouse CD73 antibody (BD Biosciences, 550738) for 5 minutes at 4 °C. After washing in MACS buffer, cells were centrifuged for 5 minutes at 300 g. The cell pellet was resuspended in 480 µL MACS buffer and 120 µL goat anti-rat IgG magnetic beads (Miltenyi Biotec, 130-048-501). The suspension was incubated for 15 min at 4 °C followed by a washing step with MACS buffer and centrifugation. Before magnetic separation, the cells were resuspended in MACS buffer and filtered through a 30-µm pre-separation filter.
The cell suspensions were applied onto a LS column fixed to a MACS separator. The column was rinsed with 3 × 3 mL MACS buffer and the flow through was collected (CD73 negative cells). The column was removed from the magnet and placed in a new collection tube. The CD73-positive fraction was eluted by loading 5 mL MACS buffer and immediately applying the plunger supplied with the column. The cells were then counted and concentrated to about 200,000 cells/µl.
Maintenance of hiPSC culture
All experiments were carried out using hiPSC-2 cell line, previously established from human dermal fibroblasts from an 8-year-old boy (gift from P. Rustin, INSERM U676, Paris) by co-transfecting OriP/EBNA1-based epi-somal vectors pEP4EO2SEN2K (3 μg), pEP4EO2SET2K (3 μg) and pCEP4-M2L (2 μg) (Addgene) via nucleofection (Nucleofector 4D, V4XP, withDT-130 program; Lonza)31, and recently adapted to feeder-free conditions23. Cells were kept at 37 °C, under 5% CO2/95% air atmosphere, and 20% Oxygen tension and 80-85% of humidity. Colonies were cultured with Essential 8™ medium (Thermo Fisher Scientific) in culture dishes coated with truncated recombinant human Vitronectin and passaged once a week23.
Generation of retinal organoids from human iPS cells
Human iPSC were differentiated towards retinal organoids following an optimized protocol based on the one published by Reichman et al.23. Briefly,hiPSC-2 cell line was expanded to 80% confluence in Essential 8™ medium were switched in Essential 6™ medium (Thermo Fisher Scientific). After 3 days, cells were moved to the Proneural medium (Supplementary Table 2). The medium was changed every 2–3 days. After 4 weeks of differentiation, neural retina-like structures grew out of the cultures and were mechanically isolated. Pigmented parts, giving rise to RPE were carefully removed. The extended 3D culture in Maturation medium (Table S1) allowed the formation of retinal organoids. Addition of 10 ng/ml Fibroblast growth factor 2 (FGF2, Preprotech) at this point favoured the growth of retinal organoids and the commitment towards retinal neurons instead of RPE lineage53. In order to promote the commitment of retinal progenitors towards photoreceptors, we specifically blocked Notch signalling for a week starting at day 42 of differentiation using the gamma secretase inhibitor DAPT (10 µM, Selleckchem)54. Floating organoids were cultured in 6 well-plates (10 organoids per well) and medium was changed every 2 days. Supplementary Table 2 summarizes the formulations for the different media used.
Infection of retinal organoids with AAV expressing Jaws
Introduction of Jaws optogene was done by one single infection at day 42 at a 5 × 1010 vg per organoid. Retinal organoids were infected with an AAV with an engineered capsid, AAV2-7m855 carrying Jaws gene under the control of mouse cone arrestin promoter and fused to the fluorescent reporter GFP (AAV2-7m8-mCAR-Jaws-GFP). For GFP only-expressing controls, an infection with AAV2-7m8-mCAR-GFP was carried out in the same manner as mentioned above.
Monolayer cultures of dissociated cells
After removal of any pigmented tissue, 70-day old retinal organoids were collected and washed three times in Ringer solution (Supplementary Table 2) before dissociation with two units of pre-activated papain at 28.7 μ/mg (Worthington) in Ringer solution for 25 min at 37 °C. Once a homogeneous cell suspension was obtained after pipetting up and down, papain was deactivated with Proneural medium (Supplementary Table 2). Cells were centrifuged and resuspended in pre-warmed Proneural medium. Dissociated retinal cells were plated onto coverslips coated with human recombinant 30 µg/cm² Laminin (Sigma-Aldrich) and 150 µg/cm² Poly-L-Ornithine in 24 well-plates56. Monolayers were incubated at 37 °C in a standard 5% CO2/95% air incubator and medium was changed every 2 days for the next 15–20 days, before immunostaining.
Preparation of cells for transplantation
At day 70 of differentiation retinal organoids were dissociated using papain as described above to obtain a single cell suspension in Proneural medium (Supplementary Table 2). Cell suspension was filtered through a 30 µm mesh (Miltenyi Biotec) to remove residual aggregates. After counting, cells were centrifuged and resuspended in Proneural medium at a concentration of 300,000 cells/µl.
RNA isolation and real-time RT-qPCR
Total RNA isolation was performed using a NucleoSpin RNA XS kit (Macherey-Nagel), according to the manufacturer’s instructions. RNA concentration and purity were determined using a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific).
Reverse transcription was carried out with 250 ng of total RNA using the QuantiTect retrotranscription kit (Qiagen). Quantitative PCR (qPCR) reactions were performed using Taqman Array Fast plates and Taqman Gene expression master mix (Thermo Fisher Scientific) in an Applied Biosystems real-time PCR machine (7500 Fast System). All samples were normalized against a housekeeping gene (18S) and the gene expression was determined based on the ΔΔCT method. Average values were obtained from at least four biological replicates. The primer sets and MGB probes (Thermo Fisher Scientific) labelled with FAM for amplification are listed in Supplementary Table 3.
Mice were sedated by intraperitoneal injection of ketamine (50 mg/kg) and xyazine (10 mg/kg) and the pupils were dilated with tropicamide drops. The mice were placed onto a heating pad to maintain the temperature at 37 °C. A drop of Lubrithal eye gel (Dechra) was used to keep the eyes hydrated during the surgery. A small glass slip was put on the eye to enable visualization through the Leica Alcon ophthalmic microscope while a syringe with a blunt, 34-gauge needle was inserted tangentially through the conjunctiva and sclera. One microlitre of cell suspension including 200,000–300,000 cells was injected between the retina and RPE, into the subretinal space, creating a bullous retinal detachment. Injections were performed bilaterally. Mice were placed into a warm chamber after the surgery until their awakening.
Tissue preparation and immunostaining
70-day old organoids were washed in PBS and fixed in 4% paraformaldehyde for 10 min at 4 °C before they were incubated overnight in 30% sucrose (Sigma-Aldrich) in PBS. Organoids were embedded in gelatin blocks (7.5% gelatin (Sigma-Aldrich), 10% sucrose in PBS) and frozen using isopentane at −50 °C.
At least 4 weeks after transplantation, mice were sacrificed by CO2 inhalation followed by a cervical dislocation. The eyeballs were removed, fixed in 4% paraformaldehyde for 30 minutes at room temperature (RT) and incubated overnight at 4 °C in PBS containing 30% (w/v) sucrose (Sigma-Aldrich). The eyes were then dissected to obtain only the back of the eye with the retina and the RPE. The samples were embedded in gelatin blocks (7.5% gelatin (Sigma-Aldrich), 10% sucrose in PBS), frozen with liquid nitrogen and stored at −80 °C.
Ten micrometres of thick sections were obtained using a Cryostat Microm and mounted on Super Frost Ultra Plus® slides (Menzel Gläser). Cryosections were washed in PBS (5 min, RT) and then permeabilised in PBS containing 0.5 % Triton X-100 during 1 h at RT. Blocking was done with PBS containing 0.2% gelatin, 0.25% Triton X-100 for 30 min at RT and incubation with primary antibodies was performed overnight at 4 °C. We used the following primary antibodies for immunostaining: hCAR (1:20,000; gift from Cheryl Craft), CRX (1:5000; Abnova, H00001406-M02), GFP (1:500; Abcam, ab13970), HNA (1:200; Millipore, MAB4383), Ki67 (1:200; BD Pharmagen, 550609), PKCα (1:100; Santa Cruz, sc-208), RCVRN (1:5000-1:2000; Millipore, AB5585), Synaptophysin (1:200; Sigma, SAB4502906). The antibodies used are also listed in Supplementary Table 4. After incubation with primary antibodies, sections were washed with PBS containing 0.25% Tween20 and incubated with fluorochrome-conjugated secondary antibodies (1:500; Thermo Fisher Scientific) for 1 h at RT. After successive washing in PBS-Tween20, nuclei were counterstained with DAPI (4′-6′-diamino-2-phenylindole, dilactate; Invitrogen-Molecular Probe, Eugene, OR) at a 1:1000 dilution. Samples were further washed in PBS and dehydrated with 100% ethanol before mounting using fluoromount Vectashield (Vector Laboratories).
FISH for Y chromosome detection
For combined chromosomal fluorescence in situ hybridization (Y chromosome FISH) and immunohistochemistry, retinas from female Cpfl1/Rho−/− mice transplanted with male donor-derived rod precursors (N = 5) were collected 4 weeks post-surgery, fixed for 1 h at 4 °C with freshly prepared 4% paraformaldehyde (Merck Millipore), incubated in 30% sucrose overnight, followed by cryopreservation. After embedding and freezing in OCT medium, cryosections of 12 µm were rehydrated with 10 mM sodium citrate buffer pH 6, antigen retrieval performed (80 °C, 25 min). Sections were washed in PBS for 5 min and incubated with a primary antibody against GFP (1:500; Abcam, ab13970) overnight at RT, followed by incubation with secondary antibody conjugated to AlexaFluor 488 (1:1000; Jackson Immunoresearch, 103545155) overnight at RT. Next, slides were post-fixed in 2% PFA for 10 min, pre-treated with 50% formamide for 1 h at RT, then hybridization of the XMP Y orange probe (Metasystems, D-1421-050-OR) to the Y chromosome was performed. To allow the probe to penetrate the tissue, samples were incubated for 3 h at 45 °C in a HybEZ II oven. Then, samples were transferred to a hot block at 80 °C for 5 min, to denature DNA. Afterwards, probes were hybridized with DNA for 2 days at 37 °C. Posthybridization consisted of 3 × 15 min washes with 2× SCC at 37 °C and 2 × 5 min stringency washes with 0.1× SCC at 60 °C. Finally, sections were counterstained with DAPI (1:15,000; Sigma). The samples were imaged and quantified using structured illumination microscopy (SIM; ApoTome, Zeiss).
For information on antibodies used, see Supplementary Table 4.
Quantification of YFP+ cells after transplantation
Tranplanted host eyes (N = 6) were processed and cryosectioned as described for the Y chromosome FISH experiment, and subsequently stained for GFP (1:500; Abcam, ab13970) and photoreceptor specific marker RCVRN (1:5000; Millipore, AB5585), followed by secondary antibody staining (1:1000; Jackson Immunoresearch) Every fourth serial section from whole experimental retinas was used to quantify the total amount of YFP+ photoreceptors. Cells were counted from images obtained with the NanoZoomer microscope (Hamamatsu Photonics). Following these cell counts, the resulting value was multiplied by four to estimate the total amount of labelled cells per retina.
For information on antibodies used, see Supplementary Table 4.
Nuclear size measurements
Measurements of the nuclear size were performed with FIJI software (NIH) on immunostained sections of rd1 transplanted retinas and compared with the values in wild-type mice.
Immunofluorescence was observed using a Leica DM6000 microscope (Leica microsystems) equipped with a CCD CoolSNAP-HQ camera (Roper Scientific) or using an inverted or upright laser scanning confocal microscope (FV1000, Olympus) with 405, 488, 515 and 635 nm pulsing lasers. The images were acquired sequentially with the step size optimized based on the Nyquist–Shannon theorem. The analysis was conducted in FIJI (NIH). Images were put into a stack, Z-sections were projected on a 2D plane using the MAX intensity setting in the software’s Z-project feature, and the individual channels were merged.
Images of Y chromosome labelled retinas were acquired using SIM (ApoTome, Zeiss). Samples stained to perform quantification of surviving YFP+ photoreceptors were imaged with the NanoZoomer microscope (Hamamatsu Photonics).
Light stimulation of NpHR-positive, Jaws-positive, and control cells
Light-triggered responses were measured in donor cells before transplantation—in vivo in AAV-injected wild-type donor mice at P12 for NpHR+, and in retinal organoids and monolayer cultures from dissociated organoids for Jaws+ cells. In order to measure light responses we used a monochromatic light source (Polychrome V, TILL photonics). After patching the cells we first stimulated them with a pair of 590 nm full-field light pulses. Then the activity spectrum was measured by using light flashes ranging from 400 to 650 nm (separated by 25 nm steps). Finally we generated light pulses at different frequencies ranging between 2 and 30 Hz in order determine the temporal response properties of NpHR and Jaws in AAV-transduced cells. Stimulation and analysis were performed using custom-written software in Matlab (Mathworks) and Labview (National Instruments). We used light intensities ranging between 1 × 1016 and 3.2 × 1017 photons cm−2 s−1.
Live two-photon imaging and patch-clamp recordings
Donor mouse retina (P12), retinal organoids or monolayer cultures from dissociated organoids were placed in the recording chamber of the microscope at 36 °C in oxygenated (95% O2/5% CO2) Ames medium (Sigma-Aldrich) during the whole experiment. Transplanted mice were sacrificed by CO2 inhalation followed by quick cervical dislocation, and eyeballs were removed. Retinae from Cpfl1/Rho−/− or rd1 mice were isolated in oxygenated (95% O2/5% CO2) Ames medium and whole mount retinas with ganglion cell side down were placed in the recording chamber of the microscope at 36 °C for the duration of the experiment for both live two-photon imaging and electrophysiology.
A custom-made two-photon microscope equipped with a 25x water immersion objective (XLPlanN-25 × -W-MP/NA1.05, Olympus) equipped with a pulsed femto-second laser (InSight™ DeepSee™ - Newport Corporation) were used for imaging and targeting AAV-transduced fluorescent photoreceptor cells (eYFP+ or GFP+ cells). Two-photon images were acquired using the excitation laser at a wavelength of 930 nm. Images were processed offline using ImageJ (NIH). A CCD camera (Hamamatsu Corp.) was also used to visualize the donor cells or the retina under infrared light.
For patch-clamp recordings, AAV-transduced fluorescent cells were targeted with a patch electrode under visual guidance using the reporter tag’s fluorescence. Whole-cell recordings were obtained using the Axon Multiclamp 700B amplifier (Molecular Device Cellular Neurosciences). Patch electrodes were made from borosilicate glass (BF100-50-10, Sutter Instrument) pulled to 7–10 MΩ and filled with 115 mM K Gluconate, 10 mM KCl, 1 mM MgCl2, 0.5 mM CaCl2, 1.5 mM EGTA, 10 mM HEPES, and 4 mM ATP-Na2 (pH 7.2). Photocurrents were recorded while voltage-clamping cells at a potential of −40 mV. Some cells were also recorded in current-clamp (zero) configuration, hence allowing us to monitor the membrane potential during light stimulations.
A monochromatic light source (Polychrome V, TILL photonics) was used to stimulate cells during electrophysiological experiments and hence record photocurrents or changes in cells membrane potential. First, in order to measure the activity spectrum of NpHR and Jaws, we used 300 ms light flashes ranging from 650 to 400 nm (25 nm steps; interstimulus interval 1.5 s) at a constant light intensity of 1.2 × 1016 photons cm−2 s−1. Then this light source was used at a constant wavelength of 590 nm to generate light pulses at different frequencies (ranging from 2 to 30 Hz) in order determine the temporal response properties of optogenetic proteins used. Stimuli were generated using custom-written software in LabVIEW (National Instruments) and output light intensities were calibrated using a spectrophotometer (USB2000+, Ocean Optics).
Multi-electrode array recordings and data analysis
The mice were euthanized, the retinas isolated, cut each in two pieces and placed in Ames medium bubbled with 95% O2 and 5% CO2. Each piece was mounted separately on a cellulose membrane soaked overnight in poly-L-lysin and gently pressed against a 60-µm electrode spacing 252 channel multi-electrode array chip (256MEA60/10iR, Multi Channel Systems) with RGCs facing the electrodes. The piece remained perfused with oxygenated Ames medium at 34 °C throughout the experiment. Full field light stimuli were applied with a Polychrome V monochromator (TILL Photonics) driven by a STG2008 stimulus generator (Multichannel Systems) using custom written stimuli in MC_Stimulus II (MC_Stimulus II Version 3.4.4, Multichannel Systems).
The basic stimulus pattern applied was 10 repetitions of 2-s stimuli of 580 nm light (close to excitation maximum for NpHR and Jaws) and intensity of 7 × 1016 photons cm−2 s−1, with 10 s intervals. To assess temporal dynamics of responding cells, stimuli ranging from 1 ms to 2 s were played to the retina. Action spectrum of optogenetic protein-expressing cells was examined by playing sets of stimuli of different wavelengths (450 nm, 500 nm, 550 nm, 580 nm, 600 nm, 650 nm; 10 stimuli of 2 s with 10 s intervals for each wavelength). To determine sensitivity of responding cells, stimuli of lower intensities were also used (1 × 1014, 7 × 1014, 2 × 1015 and 9 × 1015 photons cm−2 s−1). During the experiments aiming to show that the light responses are really coming from the ONL, we perfused the tissue with L-AP4 (50 μM) for at least 20 min before the recordings in order to block input from photoreceptors to ON bipolar cells. This was followed by at least 15 min rinse with Ames medium and another set of light stimulation to observe whether the response returned.
Data were acquired using the MC_Rack software (MC_Rack v4.5, Multi Channel Systems). RGC responses were amplified and sampled at 20 kHz. Data was then filtered with a 200 Hz high pass filter and individual channels were spike sorted using template matching and cluster grouping based on principal component analysis of the waveforms in Spike2 software v.7 (Cambridge Electronic Design Ltd). The raster plots and peristimulus time histogram data (bin size of 10 ms) were constructed in MATLAB using custom scripts from spike-sorted channels and further processed in Adobe Illustrator CS4 (Adobe Systems) for presentation.
Maximum firing rate for each responding cell was measured in the 2 s after the onset (for ON-responding cells) or 2 s after the offset (for OFF-responding cells) of the stimulus. The number of cells and mice that were used for quantitative analysis are stated in Figure legends. Error bars were calculated over cells.
For light-avoidance behaviour, we used a custom-made dark-light box22,57 of dimensions 36 cm × 20 cm × 18 cm, divided longitudinally into two equal sized compartments with a non-transparent wall with a 7 cm × 5 cm hole in the middle. The light compartment was equipped with eight 590 nm LEDs (Cree XP-E, amber, Lumitronix) 3 cm from the bottom of the box. A light intensity of 2.05 × 1016 photons cm−2 s−1 was used for all the experiments. The mice were habituated in the dark for at least 2 h prior the testing. Each mouse was introduced into the light compartment and was left in the box for at least 5 min before the start of illumination. The lights were turned on when the mouse was in the light compartment and were left on for at least 5 min. The behaviour of the mice was recorded with a camera and subsequently analyzed manually by recording the times spent in each compartment after the start of illumination, and using the Smart Vision Tracking Software (Harvard Apparatus). The mouse’s head was used to define the compartment it occupied.
Data was analyzed with GraphPad Prism and it was expressed as mean ± standard error of mean (SEM). Comparisons between values were analyzed using unpaired two-tailed non-parametric Mann––Whitney Student’s test. A level of p < 0.05 was considered significant. The labels used were: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
The data that support the findings of this study are available from the corresponding author upon reasonable request. The source data underlying Figs. 1i, 2a, d, 3c, e, g, i, k, 4f, 5d, e, h, j, resting membrane potential values (RPM), and Supplementary Figs. 3c, 6b, e, j and 8c are provided as a Source Data file.
The MATLAB codes that were used to represent MEA raster and PSTH data are available from the corresponding author upon reasonable request.
Jayakody, S. A., Gonzalez-Cordero, A., Ali, R. R. & Pearson, R. A. Cellular strategies for retinal repair by photoreceptor replacement. Prog. Retin. Eye Res. 46, 31–66 (2015).
West, E. L., Pearson, R. A., MacLaren, R. E., Sowden, J. C. & Ali, R. R. Cell transplantation strategies for retinal repair. Prog. Brain Res. 175, 3–21 (2009).
Santos-Ferreira, T. F., Borsch, O. & Ader, M. Rebuilding the missing Part-A review on photoreceptor transplantation. Front. Syst. Neurosci. 10, 105 (2017).
Eberle, D. et al. Outer segment formation of transplanted photoreceptor precursor cells. PloS ONE 7, e46305 (2012).
Barber, A. C. et al. Repair of the degenerate retina by photoreceptor transplantation. Proc. Natl Acad. Sci. USA 110, 354–359 (2013).
Singh, M. S. et al. Reversal of end-stage retinal degeneration and restoration of visual function by photoreceptor transplantation. Proc. Natl Acad. Sci. USA 110, 1101–1106 (2013).
Mandai, M. et al. iPSC-derived retina transplants improve vision in rd1 end-stage retinal-degeneration mice. Stem Cell Rep. 8, 1112–1113 (2017).
Iraha, S. et al. Establishment of immunodeficient retinal degeneration model mice and functional maturation of human ESC-derived retinal sheets after transplantation. Stem Cell Rep. 10, 1059–1074 (2018).
Strauss, O. The retinal pigment epithelium in visual function. Physiological Rev. 85, 845–881 (2005).
Wright, A. F., Chakarova, C. F., Abd El-Aziz, M. M. & Bhattacharya, S. S. Photoreceptor degeneration: genetic and mechanistic dissection of a complex trait. Nat. Rev. Genet. 11, 273–284 (2010).
Milam, A. H., Li, Z. Y. & Fariss, R. N. Histopathology of the human retina in retinitis pigmentosa. Prog. Retin. Eye Res. 17, 175–205 (1998).
Chiba, C. The retinal pigment epithelium: an important player of retinal disorders and regeneration. Exp. Eye Res. 123, 107–114 (2014).
Gradinaru, V., Thompson, K. R. & Deisseroth, K. eNpHR: a Natronomonas halorhodopsin enhanced for optogenetic applications. Brain Cell Biol. 36, 129–139 (2008).
Koso, H. et al. CD73, a novel cell surface antigen that characterizes retinal photoreceptor precursor cells. Investigative Ophthalmol. Vis. Sci. 50, 5411–5418 (2009).
Eberle, D., Schubert, S., Postel, K., Corbeil, D. & Ader, M. Increased integration of transplanted CD73-positive photoreceptor precursors into adult mouse retina. Investigative Ophthalmol. Vis. Sci. 52, 6462–6471 (2011).
Santos-Ferreira, T. et al. Stem cell-derived photoreceptor transplants differentially integrate into mouse models of cone-rod dystrophy. Investigative Ophthalmol. Vis. Sci. 57, 3509–3520 (2016).
Viczian, A., Sanyal, S., Toffenetti, J., Chader, G. J. & Farber, D. B. Photoreceptor-specific mRNAs in mice carrying different allelic combinations at the rd and rds loci. Exp. Eye Res. 54, 853–860 (1992).
Carter-Dawson, L. D., LaVail, M. M. & Sidman, R. L. Differential effect of the rd mutation on rods and cones in the mouse retina. Investigative Ophthalmol. Vis. Sci. 17, 489–498 (1978).
Crevier, D. W. & Meister, M. Synchronous period-doubling in flicker vision of salamander and man. J. Neurophysiol. 79, 1869–1878 (1998).
European Parliament and Council of the European Union. Directive 2006/25/EC on artificial optical radiation. Off. J. Eur. Opean Union L 114, 38–59 (2006).
International Commission on Non-ionizing Radiation Protection. ICNIRP guidelines on limits of exposure to incoherent visible and infrared radiation. Health Phys. 105, 74–96 (2013).
Bourin, M. & Hascoet, M. The mouse light/dark box test. Eur. J. Pharmacol. 463, 55–65 (2003).
Reichman, S. et al. Generation of storable retinal organoids and retinal pigmented epithelium from adherent human iPS cells in xeno-free and feeder-free conditions. Stem Cells, https://doi.org/10.1002/stem.2586 (2017).
Chuong, A. S. et al. Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nat. Neurosci. 17, 1123–1129 (2014).
Khabou, H. et al. Noninvasive gene delivery to foveal cones for vision restoration. JCI insight 3, https://doi.org/10.1172/jci.insight.96029 (2018).
Garita-Hernandez, M. et al. Optogenetic light sensors in human retinal organoids. Front. Neurosci. 12, 789 (2018).
Santos-Ferreira, T. et al. Retinal transplantation of photoreceptors results in donor-host cytoplasmic exchange. Nat. Commun. 7, 13028 (2016).
Pearson, R. A. et al. Donor and host photoreceptors engage in material transfer following transplantation of post-mitotic photoreceptor precursors. Nat. Commun. 7, 13029 (2016).
Singh, M. S. et al. Transplanted photoreceptor precursors transfer proteins to host photoreceptors by a mechanism of cytoplasmic fusion. Nat. Commun. 7, 13537 (2016).
Dalkara, D., Goureau, O., Marazova, K. & Sahel, J. A. Let There Be Light: Gene and Cell Therapy for Blindness. Hum. Gene Ther. 27, 134–147 (2016).
Reichman, S. et al. From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium. Proc. Natl Acad Sci. USA 111, 8518–8523 (2014).
Wright, L. S., Phillips, M. J., Pinilla, I., Hei, D. & Gamm, D. M. Induced pluripotent stem cells as custom therapeutics for retinal repair: progress and rationale. Exp. Eye Res. 123, 161–172 (2014).
Zhong, X. et al. Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs. Nat. Commun. 5, 4047 (2014).
Wiley, L. A. et al. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness. Sci. Rep. 6, 30742 (2016).
Gonzalez-Cordero, A. et al. Recapitulation of human retinal development from human pluripotent stem cells generates transplantable populations of cone photoreceptors. Stem Cell Rep. 9, 820–837 (2017).
Gagliardi, G. et al. Characterization and transplantation of CD73-positive photoreceptors isolated from human iPSC-derived retinal organoids. Stem Cell Rep. 11, 665–680 (2018).
Lakowski, J. et al. Isolation of human photoreceptor precursors via a cell surface marker panel from stem cell-derived retinal organoids and fetal retinae. Stem Cells 36, 709–722 (2018).
Barnea-Cramer, A. O. et al. Function of human pluripotent stem cell-derived photoreceptor progenitors in blind mice. Sci. Rep. 6, 29784 (2016).
Zhu, J., Cifuentes, H., Reynolds, J. & Lamba, D. A. Immunosuppression via loss of IL2rgamma enhances long-term functional integration of hESC-derived photoreceptors in the mouse retina. Cell Stem Cell 20, 374–384 (2017). e375.
Shirai, H. et al. Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration. Proc. Natl Acad. Sci. USA 113, E81–E90 (2016).
Sparrow, J. R., Hicks, D. & Hamel, C. P. The retinal pigment epithelium in health and disease. Curr. Mol. Med. 10, 802–823 (2010).
Nickerson, P. E. B., Ortin-Martinez, A. & Wallace, V. A. Material exchange in photoreceptor transplantation: updating our understanding of donor/host communication and the future of cell engraftment science. Front. Neural Circuits 12, 17 (2018).
Busskamp, V. et al. Genetic reactivation of cone photoreceptors restores visual responses in retinitis pigmentosa. Science 329, 413–417 (2010).
Azoulay-Sebban, L. Etude des corrélations anatomiques et fonctionnelles au cours de la rétinopathie pigmentaire: identification et validation de nouveaux marqueurs prédictifs Sciences Recherche Clinique thesis, Paris 6, (2015).
Li, Z. Y., Possin, D. E. & Milam, A. H. Histopathology of bone spicule pigmentation in retinitis pigmentosa. Ophthalmology 102, 805–816 (1995).
Athanasiou, D. et al. The cell stress machinery and retinal degeneration. FEBS Lett. 587, 2008–2017 (2013).
Cideciyan, A. V. Leber congenital amaurosis due to RPE65 mutations and its treatment with gene therapy. Prog. Retin. Eye Res. 29, 398–427 (2010).
Chang, B. et al. Retinal degeneration mutants in the mouse. Vis. Res. 42, 517–525 (2002).
Humphries, M. M. et al. Retinopathy induced in mice by targeted disruption of the rhodopsin gene. Nat. Genet. 15, 216–219 (1997).
LaVail, M. M. & Sidman, R. L. C57BL-6J mice with inherited retinal degeneration. Arch. Ophthalmol. 91, 394–400 (1974).
Choi, V. W., Asokan, A., Haberman, R. A. & Samulski, R. J. Production of recombinant adeno-associated viral vectors. Curr. Protoc. Hum. Genet. Chapter 12, Unit 12 19 (2007).
Aurnhammer, C. et al. Universal real-time PCR for the detection and quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Hum. Gene Ther. Methods 23, 18–28 (2012).
Fuhrmann, S. Eye morphogenesis and patterning of the optic vesicle. Curr. Top. Develop. Biol. 93, 61–84 (2010).
Jadhav, A. P., Mason, H. A. & Cepko, C. L. Notch 1 inhibits photoreceptor production in the developing mammalian retina. Development 133, 913–923 (2006).
Dalkara, D. et al. In vivo-directed evolution of a new adeno-associated virus for therapeutic outer retinal gene delivery from the vitreous. Sci. Transl. Med. 5, 189ra176 (2013).
Garita-Hernandez, M. et al. Hypoxia increases the yield of photoreceptors differentiating from mouse embryonic stem cells and improves the modeling of retinogenesis in vitro. Stem Cells 31, 966–978 (2013).
Sengupta, A. et al. Red-shifted channelrhodopsin stimulation restores light responses in blind mice, macaque retina, and human retina. EMBO Mol. Med. 8, 1248–1264 (2016).
Nikonov, S. S., Kholodenko, R., Lem, J. & Pugh, E. N. Jr. Physiological features of the S- and M-cone photoreceptors of wild-type mice from single-cell recordings. J. Gen. Physiol. 127, 359–374 (2006).
We thank Thierry Léveillard for providing the rd1 mice and Cheryl Craft for providing the hCAR antibody. We are thankful to Romain Caplette, Olivier Marre and Stéphane Deny for their help with the MEA recordings and analysis. We thank Abhishek Sengupta for the construction of the light/dark box. We are grateful to Mélissa Desrosiers and Camille Robert for AAV productions and the fundraising department of the Vision Institute for LCL fundraising. This study was supported by a ERC Starting Grants (OPTOGENRET, 309776/JD, REGNETHER 639888/DD), the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé et de la Recherche Médicale (INSERM), Labex-Lifesenses (D.D., J.D.), Sorbonne Université, Marie Curie CIG (334130, Retinal Gene Therapy, D.D.), INSERM, ANR grant RHU Light4Deaf (D.D.,O.G.), LCL Foundation (D.D.), Deutsche Forschungsgemeinschaft (DFG) FZT 111, Center for Regenerative Therapies Dresden, FZT 111 Cluster of Excellence (M.A.), DFG Grant AD375/6-1 (M.A.), BMBF Research Grant 01EK1613A (M.A.).
Sorbonne Universite, Centre National De La Recherche Scientifique and INSERM have submitted a patent application (PCT/EP2017/074125) based on the work covered in this manuscript. J.D., M.A., A.C., M.G.-H., M.L., D.D., O.G. and J.-A.S are affiliated with these organisations and the patent. The remaining authors declare no competing interests.
Peer review information Nature Communications thanks John Flannery and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
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Garita-Hernandez, M., Lampič, M., Chaffiol, A. et al. Restoration of visual function by transplantation of optogenetically engineered photoreceptors. Nat Commun 10, 4524 (2019). https://doi.org/10.1038/s41467-019-12330-2
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