A central problem in speciation is the origin and mechanisms of reproductive barriers that block gene flow between sympatric populations. Wind-pollinated plant species that flower in synchrony with one another rely on post-pollination interactions to maintain reproductive isolation. In some locations in Mexico, sympatric populations of domesticated maize and annual teosinte grow in intimate associate and flower synchronously, but rarely produce hybrids. This trait is typically conferred by a single haplotype, Teosinte crossing barrier1-s. Here, we show that the Teosinte crossing barrier1-s haplotype contains a pistil-expressed, potential speciation gene, encoding a pectin methylesterase homolog. The modification of the pollen tube cell wall by the pistil, then, is likely a key mechanism for pollen rejection in Zea and may represent a general mechanism for reproductive isolation in grasses.
Reproductive isolation is essential for the origin of new species1. Wind-pollinated plant species, like those of the grass family, rely on post-pollination barriers to prevent hybrid production, including both pollen–pistil interactions that prevent fertilization and post-fertilization hybrid abortion or sterility2,3,4. Maize (Zea mays ssp. mays) was domesticated from annual teosinte (Z. mays ssp. parviglumis) in the Balsas River valley of Mexico about 9000 years ago5. Despite this recent divergence and the full compatibility of some maize and teosinte populations, other sympatric populations of annual teosinte grow together and flower synchronously with domesticated maize, but rarely produce hybrids6,7. The Teosinte crossing barrier1-s (Tcb1-s) haplotype, originally identified from strains of Z. mays ssp. mexicana teosinte, confers this cross-incompatibility trait to these plants.
In Z. mays, haplotypes at three loci, Tcb1-s, Gametophyte factor1-s (Ga1-s) and Gametophyte factor2-s (Ga2-s) confer unilateral cross-incompatibility against varieties carrying the tcb1 (or ga1 or ga2, respectively) haplotype. While Ga1-s and Ga2-s are widespread in domesticated maize, Tcb1-s is almost exclusively found in wild teosinte populations, with the exception of one ancient Maiz Dulce sweet corn variety from Mexico8. Tcb1-s was first described in teosinte subspecies mexicana Collection 48703 from central and southern Mexico6,7,9. Other collections of teosinte of both mexicana and parviglumis subspecies from the central Mexican plateau also carry Tcb1-s10. Since Tcb1-s is unilateral it can be crossed into maize and functions as it does in teosinte to reject standard maize pollen7.
Tcb1-s females block fertilization by maize (tcb1 type) pollen by restricting pollen tube growth in the pistil (silk)4. This function makes Tcb1 a candidate speciation gene contributing to isolation of diverging maize and teosinte populations, as wild teosinte populations respond to the pressure of cultivated, closely related varieties of domesticated maize10. In the reciprocal cross, teosinte pollen is able to fertilize maize, although at a slight disadvantage when in competition with maize pollen7. The Tcb1-s locus contains male (Tcb1-male) and female (Tcb1-female) genes, which are tightly linked but separable by recombination4. Thus, there are four functional haplotypes at this locus (Supplementary Table 1 for gene content and origin): Tcb1-s has both functional male and female genes, Tcb1-m has only the functional male gene4,10, Tcb1-f has only the functional female gene, and the tcb1 haplotype found in almost all maize lines has neither of the two functional genes. In teosinte, Tcb1-f activity in the silks prevents fertilization by maize (tcb1) pollen, while Tcb1-m activity in pollen enables fertilization of Tcb1-f or Tcb1-s females4.
There are two alternative explanations of rejection of tcb1 pollen: (1) tcb1 pollen expresses an allele of Tcb1-m that is recognized by the Tcb1-female gene leading to directed inhibition of tcb1 but not the unrecognized Tcb1-m pollen; or (2) Tcb1-m confers a function to pollen not present in tcb1 allowing it to overcome the barrier set up indiscriminately in Tcb1-f pistils. This second case is preferred since pollen heterozygous for Tcb1-s and tcb1 haplotypes (using a trisomic line carrying a duplication of the short arm of Chromosome 4) fertilizes Tcb1-s females, indicating that the Tcb1-female:Tcb1-male match overcomes the Tcb1-female:tcb1-male mismatch (the situation is similar for the Ga1 and Ga2 systems)11,12.
This study demonstrates that the Tcb1-female gene encodes a pistil-expressed pectin methylesterase38 (PME38) homolog. The identity of this gene suggests a mechanism for reproductive isolation in diverging plant populations of maize and some teosintes, in which the pollen tube cell wall is modified by the female thus preventing continued pollen tube growth and delivery of the sperm cells. Agriculturally, this work may facilitate reproductive isolation of specialty crops, and enrichment of crop plant germplasm by overcoming barriers to crossing with wild relatives13.
Fine mapping analysis and mutant screen of Tcb1-s
To clone the Tcb1 genes, fine mapping of Tcb1-s::Col48703 haplotype was performed based on a tcb1 backcross population of ~15,000 chromosomes. Using the maize B73 genome as a reference14, the Tcb1 locus was delimited to a region spanning 480 kb on the short arm of Chromosome 4. Within this region, there are 10 annotated genes. However, all of these were ruled out as candidates for Tcb1 functions because they either had identical sequence with identical expression levels between tcb1 and Tcb1-s haplotypes or no expression in the silk or pollen in Tcb1-s or tcb1 (mapping markers included in Supplementary Table 2). The Tcb1 genes, therefore, are likely absent from the maize genome. This is not surprising considering the widespread structural variations in genomes between maize lines and between teosinte populations15. Using gene content information from B73, clones were isolated and sequenced from a Bacteria Artificial Chromosome (BAC) library of the Tcb1-s::Col48703 haplotype to identify gene models present in the Tcb1-s haplotype that are absent in maize reference genomes. These BACs were assembled into three contigs with two gaps in the Tcb1-s region. Four new gene models from this assembly could be eliminated as candidates based on lack of expression or identical sequence coupled with identical expression to unlinked maize genes. However, the data did provide more mapping markers for delineating the Tcb1-f and Tcb1-m gene regions.
To identify Tcb1-f knockout mutants, maize lines homozygous for the Tcb1-s::Col48703 haplotype and carrying active Mutator transposons were crossed to maize inbred A195 su1. The progeny are expected to be heterozygous for Tcb1-s with su1 ~6 cM away and in repulsion7. Due to the rejection of the tcb1 pollen (which is predominantly su1), about 3% of the kernels in every ear with functional Tcb1-f were expected to be su1 homozygotes in this open-pollinated population, while any ears without a crossing barrier that fail to exclude tcb1 pollen were predicted to segregate su1 at 25%. Out of a population of ~6000 individuals, two exceptional ears were found. One ear segregated for 25.6% sugary. This allele is termed tcb1-f(KO1). The second isolate contained a sector of about 45 kernels within which the segregation was one-fourth sugary despite sugary segregating at ~3% over the rest of the ear. This allele is termed tcb1-f(KO2) and was recovered from the loss-of-function sector. Mixed pollination tests with the progeny of both individuals show that the loss of function is heritable, and both variants fertilized a Tcb1-s/tcb1 strain normally, indicating the retention of the male function of Tcb1-s (Tcb1-f mutated, but Tcb1-m intact) (Fig. 1). In the case of tcb1-f(KO2), progeny of seeds within the loss-of-function side of the ear inherited the knock-out, while those on the other side of the ear inherited fully functional Tcb1-s.
Tcb1-female encodes a PME38 homolog
RNA from silks of four genotypes were subjected to short read RNA-seq: the two knockout mutants, a standard maize inbred line W22 (genotype tcb1), and a functional Tcb1-s line (a W22 subline to which the Tcb1-s::Col48703 haplotype had been introduced by backcrossing). Transcript models were assembled de novo from the RNA-seq reads, and expression levels of genes were compared between lines. One gene, encoding a maize PME38 homolog, was identified as a candidate for the Tcb1-f gene. This gene is highly expressed in Tcb1-s silks (with a peak read depth of ~100,000) compared to the standard maize tcb1 W22 silks, tcb1-f(KO2) silks (maximum read depths of ~100) and tcb1-f(KO1) silks (maximum read depth of ~10,000 for the 5′ end and ~100 for the 3′ end of the transcript model) (Fig. 2a). qRT-PCR confirmed this expression difference (Fig. 2b). A BAC clone from the Tcb1-s library carrying this PME38 homolog was identified by PCR. By comparing mRNA and BAC sequences, a 99-base pair intron was identified in Tcb1-f (Supplementary Fig. 1). Comparison of this gene to maize sequences (maizegdb.org and NCBI) by BLAST demonstrated that it is not present in the maize B73 reference genome, which is consistent with the mapping data. Its closest homologs are pseudogenes located at the ga1 locus in both B73 and W22 and a silk-expressed (putative Ga1-f) gene in Ga1-s maize16. It is likely that the small number of reads from tcb1 RNA-Seq that map to the gene are a consequence of miss-assignment of reads from the poorly expressed pseudogenes.
In addition to the two knockout mutants above, several other lines derived from Tcb1-s::Col48703 have lost female barrier function. One was recovered during early backcrossing of the Tcb1-s::Col48703 haplotype into maize6. Mixed pollination confirmed this is a Tcb1-male only plant (Supplementary Fig. 2). Additionally, two independent Tcb1-s lines were isolated in which the barrier gradually lost strength over 10 generations of backcrossing to maize4, and are named as tcb1-f::silent lineage1 (tcb1-f::sl1) and tcb1-f::silent lineage2 (tcb1-f::sl2), based on the progressive manner of the barrier loss. This PME38 has much lower expression in these three lines than Tcb1-s::Col48703 (Fig. 2b). Expression of the PME38 was also tested on two Tcb1-m lines from the mapping population, which lost the Tcb1-female gene by recombination. Again, the PME38 expression was much lower than in Tcb1-s lines (Fig. 2b).
A PCR-based derived Cleaved Amplified Polymorphic Sequence (dCAPS) marker was designed for the PME38 gene (Supplementary Fig. 3). This marker was tested on the 15 closest recombinants from the mapping population of ~15,000 individuals (including four recombinants between the Tcb1-f and Tcb1-m genes)4. Of the 15 plants, 6 carried Tcb1-f and blocked maize pollen, and 9 lacked the barrier. Results showed that all the six recombinants that carry the barrier had the PME38 gene, while in all nine recombinants that are receptive to maize pollen, this PME38 was absent, demonstrating tight linkage between the PME38 and Tcb1-f barrier function.
RNA-seq data suggest that the mutation in the PME38 lies in the first exon in tcb1-f(KO1) (Fig. 2a). Quite differently, in tcb1-f(KO2) mutant silk RNA-seq reads had the same low level of expression as tcb1 silks along the whole PME38 transcript, and the whole coding of PME38 can be PCR-amplified from tcb1-f(KO2) genomic DNA but not tcb1-f(KO1) (Supplementary Fig. 4). Whole genome resequencing of both mutants identified a Hopscotch retrotransposon insertion in the first exon in tcb1-f(KO1), a mutation unrelated to Mutator transposons, but deletions and non-Mutator insertions occur in these lines17. This insertion is close to the site where the PME38 expression drops sharply. PCR spanning both ends of the insertion confirmed the insertion event and the border sequences (Supplementary Fig. 5). In contrast, in tcb1-f(KO2), the PME38 gene was fully assembled, consistent with the PCR data. The tcb1-f(KO2) allele then could either be mutated in a regulatory region, potentially hundred kilobases away from the coding region, or could be an epi-allele. Similarly, no mutations were found in the coding region in the Tcb1-m line or the tcb1-f::sl1 or tcb1-f::sl2 lines described above.
Reversion of silenced tcb1-f loss-of-funtion lines
Since several of the loss-of-function lines did not carry mutations in the PME38 coding region, tests were performed to determine if they might be epi-alleles and so could revert back to full-strength Tcb1-s. The tcb1-f(KO2), tcb1-f::sl1, and tcb1-f::sl2 lines were tested for reversion to Tcb1-s in double mutants with the mediator of paramutation1 (mop1) mutation. MOP1 encodes a RNA-dependent RNA polymerase and is a key component of RNA-directed DNA methylation18. mop1 mutations reactivate silenced genes and affect broad developmental programs19. Re-activation of the Tcb1-f function was rare. In only ~14–22% of the mop1 females tested, did the loss-of-function plants show some recovery of Tcb1-f function. Pollen competition experiments were performed for full strength, wild-type Tcb1-s females, tcb1 females, and the tcb1-f loss of function lines without sequence changes (tcb1-f(KO2), tcb1-f::sl1, tcb1-f::sl2, and a few Tcb1-m) (Fig. 3 and Source Data file). All of the Tcb1-s ears tested showed strong preference for Tcb1-s pollen (0–7% kernels from tcb1 pollen regardless of the ratio of the two pollen types in the mix as indicated by the neutral ear) with the kernel ratio on the test ear and control ear being different from each other at p < 0.0001 (two-tailed Fisher exact test) (Fig. 3b). Of the 36 mop1; tcb1-f loss of function females tested only one (tcb1-f::sl1; mop1) had as strong of a pollen preference as full strength Tcb1-s females, but five had a difference between the test and control ears at p < 0.0001 and an additional three females could be included if the stringency was relaxed to p < 0.01 (two-tailed Fisher exact test) (Fig. 3b). These partial revertants included plants of lines tcb1-f(KO2), tcb1-f::sl1, and tcb1-f::sl2. Of the 12 loss of function plants tested that were heterozygous wild-type for mop1, none of the plants passed the more stringent p < 0.0001 threshold and one passed the less stringent p < 0.01 threshold (two-tailed Fisher exact test). Additionally, none of the 13 comparisons between two tcb1 ears had significant differences between the two ears.
A subset of homozygous mop1 tcb1-f::sl2 plants were tested at random for PME38 expression in silks prior to pollination. Among the seven tested plants, one plant, yx57-13, showed increased expression, about 400-fold higher expression compared to that of the standard W22 maize and eight times higher than tcb1-f::sl2 plants (Fig. 3c). This plant was the only one of the seven tested for expression that recovered the ability to reject tcb1 pollen, although not as efficiently as full strength Tcb1-s plants, which have still higher expression of the PME38 gene. This indicates a correlation between PME38 expression level and the female barrier strength with the expression level in tcb1-f::sl1 and tcb1-f::sl2 being below the expression threshold for producing a detectable barrier. This correlation and the Hopscotch insertion in tcb1-f(KO1) supports this PME38 as the Tcb1-f gene.
In addition to the Tcb1-s::Col48703 strain descried above, three other teosinte-derived Tcb1-s lines, two from ssp. mexicana and one ssp. parviglumis10, were tested for Tcb1-f/PME38 expression in silk tissue. In all three lines, Tcb1-f/PME38 expression levels are extremely high and comparable to that of the original central plateau TIC haplotype Tcb1-s::Col48703 (Fig. 4). Interestingly, even though none of the modern north American maize lines tested to date carry the Tcb1-s haplotype, Tcb1-s was identified in lines descended from an ancient Maiz Dulce variety, Jalisco78 that grows at intermediate altitudes in southwestern Mexico, during a survey for cross-incompatibility factors8. This is a specialty line that may have undergone selection for cross-incompatibility factors similarly to Ga1-s in maize popcorn lines but in this case to maintain a sweet corn trait. Whether this Maiz Dulce line acquired Tcb1-s from nearby teosinte populations during its origin is unknown, but maize lines from this region have been shown to have substantial introgression from ssp. mexicana teosintes20.
Diversity and relationship of Tcb1-f to other PME genes
Predicted Tcb1-f/PME38 coding sequences are identical in all five Tcb1-s lines: three mexicana accessions, one parviglumis accession, and the Maiz Dulce line. One single nucleotide polymorphism (SNP) in the intron separates these lines into two groups: one group including the parviglumis line (Col104-4a) and one mexicana line (Col109-4a), and the other group including two mexicana lines (Col48703 and Col207-5d) and the Maiz Dulce line (Supplementary Fig. 6).
The most similar gene to Tcb1-f/PME38 is a candidate PME gene for Ga1-female function. This gene, termed ZmPME3, was found to be expressed in the silks of Ga1-s, but not in ga1 silks, and maps to the Ga1 locus16. Alignment of the ZmPME3 and Tcb1-f/PME38 show that the two PMEs differ in eight amino acids (Supplementary Fig. 7). The number of polymorphisms (15 of 1296 nucleotides) between Tcb1-f/PME38 and ZmPME3 suggests that these two genes diverged ~175,000 years ago, well before the split between the mexicana and parviglumis subspecies of teosinte and just before the split between Z. mays and Zea luxurians, using calculated nucleotide substitution rates for maize21 and a calculated time since the split between mexicana and parviglumis of ~60,000 years and parviglumis and luxurians of ~140,000 years22. It will be interesting to test whether the Tcb1-male and Ga1-male genes diverged at a similar time, suggesting that the male–female genes were paired before divergence of Tcb1-s and Ga1-s.
Tcb1-s and Ga1-s are mostly cross-incompatible with one another, suggesting the male genes are also divergent. However, Tcb1-s and Ga1-s are not fully cross-incompatible. In situations where pollen rejection is not absolute, Tcb1-s pollen has a competitive advantage over tcb1 pollen on Ga1-s or Ga2-s silks. This is true for all combinations of interactions between crossing barrier loci7,12 and is consistent with them encoding related proteins. The confounding result that the behavior of pollen tubes during rejection by each system is slightly different may be explained by differences in amino acid sequence or differences in expression level or pattern between the haplotypes4. Tcb1-f/PME38 encodes a group 1 type of PME without an N-terminal pectin methylesterase inhibitor domain23, and contains a predicted signal peptide, so it has the potential to be secreted and interact directly with the pollen tube to remove methyl-esters from the pectin wall of the pollen tube. Esterified pectins are typically associated with the tip of the growing pollen tube, while de-esterified pectins are enriched distally, and there is a correlation between pectin de-esterification and increased cell wall stiffness24. Pollen cells finely tune the stiffness of the tip cell wall to sustain pollen tube elongation. Either under-supply or over-supply of PME activity can result in disturbed pollen tube growth and compromised male fertility25,26,27,28. The TCB1-F (and ZMPME3) protein falls into the Plant 1a clade of mature PME enzymes29 (Supplementary Fig. 8).
In summary, genetic and genomic data identify the Tcb1-female barrier gene as a PME38 homolog. Teosinte lines carrying Tcb1-f block maize pollen that lacks the male function provided by Tcb1-m. That the Tcb1-f gene encodes a cell wall modifying enzyme is consistent with the result that heterozygous Tcb1-s/tcb1 pollen is functional on Tcb1-s silks rather than active targeting of a pollen protein encoded by (a hypothetical maize allele of) the tcb1-m gene11. Surprisingly, it was shown that another PME family member is encoded by the Ga1-male gene30 (in a very distinct clade, Plant X2, of PME enzymes (Supplementary Fig. 8)), raising the possibility that the biochemical barrier to pollen and the ability of pollen to overcome that barrier are conferred by different classes of PME proteins. It will be interesting to test how universal this barrier mechanism is in sexual plant reproduction. The grasses also have an unusually high species diversity for a family with abiotic pollinators31. Identification of the Tcb1-female gene may facilitate research into the mechanisms of speciation in the grasses.
Maize and teosinte lines and growth conditions
All Tcb1-s lines used in this study have had the Tcb1-s haplotype introgressed from their maize or teosinte line of origin into standard maize lines W22 or B73 by backcrossing. The location of origin of these lines has been described elsewhere4,7,8,10. Plants were grown under summer field conditions at either Stanford, California or Madison, Wisconsin.
As described before7, a Central Plateau teosinte collection 487039 carrying the Tcb1-s barrier was backcrossed to the Mid-western US dent inbred W22 to incorporate the Tcb1-s locus into a maize background. This Tcb1-s strain was crossed to a chromosome 4 maize tester line virescent17 (v17) brown midrib3 (bm3) sugary1 (su1), and the F1 was then backcrossed to the same tester line. Recombinants carrying crossovers between the four visual markers were tested for the Tcb1-s male and female functions in reciprocal crosses with Tcb1-s/su1 F1 plants. PCR mapping markers were developed to refine the location of crossovers in these recombinants.
Tcb1-f knockout mutant screen
To identify loss-of-function mutants of Tcb1-female, a Ga1-m Tcb1-s active Mutator strain was crossed to maize inbred A195 su1 (tcb1), and then the progeny were grown as an open-pollinated block. Most of the progeny are expected to be heterozygous for Tcb1-s and su1 in repulsion with su1 ~6 cM away from the tcb1 locus7. Due to the rejection of the tcb1 pollen (which is predominantly su1), about 3% of the kernels in every ear with functional Tcb1-f are expected to be sugary in this open-pollinated population, while those without a crossing barrier were predicted to segregate su1 at 25%.
Mixed pollination experiments
For the mixed pollination testing of the two tcb1-f knockout mutants, two pollen donor lines and three pollen receiver lines were used. Pollen from a maize line (tcb1) that does not have the Tcb1-s barrier genes but carries the endosperm color marker R1-self color (R1-sc) will produce purple kernels after fertilization of the lines used, while pollen from the knockout plants and the Tcb1-m plants carry r1-r and produce anthocyaninless kernels that are white or yellow. After being collected from the two donors and mixed, pollen was put on the three receiving ears: (1) a Tcb1-s tester ear was used to verify the presence of the Tcb1 male function from the tcb1-f::KO pollen; (2) the tcb1-f (KO) ear was used to test the presence/absence of the female barrier function in the knockout mutant; and (3) a maize (tcb1) neutral ear was used to assay the percentage of viable pollen grains from the two donors in the mixture. Because any two tassels cannot be counted on to produce the same amounts of pollen, the ratio of the two pollen types in any mix cannot be assumed to be 1:1. Consequentially, it is essential to make a cross of each pollen mix onto a standard tcb1 maize ear (with a known pollen acceptance/function bias of 60% tcb1: 40% Tcb1-s10) to verify the ratio of functional pollen grains in the mix. The same protocol was used on the spontaneous Tcb1-m plant, except the Tcb1-m plant being tested was substituted for the KO plant. For mixed pollinations of the tcb1-f::silent lineage mop1 double-mutant plants, pollen from the same R1-sc tcb1 line and a r1-r Tcb1-s tester line was collected, mixed, and applied to the individual silent line ears and the neutral maize ears.
Silk tissue collection, RNA isolation, and cDNA synthesis
Plants for RNA isolation were grown in summer field conditions in Stanford, CA. Silk tissues were collected around 11 a.m., immediately put into liquid nitrogen in the field, and stored at −80 °C. Total RNA was isolated from silks with Trizol reagent (Invitrogen), DNase-treated, and either subjected to Illumina short read paired end RNA-seq, or used to synthesize the first strand cDNA with the Superscript IV RT kit (Invitrogen).
RT-PCR was performed on a Roche Lightcycler 480II (Roche Diagnostics). Each line/genotype had three biological replicates, and each in turn had three technical replicates. For cases where expression levels in individual plants is shown three technical replicates were used. Tubulin (Zm00001d033850) was used as a reference gene. In each line, relative expression levels were obtained by comparing Tcb1-f/PME38 to tubulin. Primers are listed in Supplementary Table 3.
Sequencing, assembly, and analysis
All the RNA and DNA sequencing works were done with Illumina Paired-end sequencing by Novogene (CA, USA). RNA-seq reads from all samples were combined and de novo assembled with Trinity v2.4.032. The gene in contig DN33598_c7_g3_i1 was identified as the Tcb1-f candidate gene due to its extremely high expression in the functional Tcb1-s line and the near lack of expression in the KO mutants and a standard W22 maize line. PCR primers were designed based on the DN33598_c7_g3_i1 sequence, and one BAC clone was isolated from a library made from a maize line into which the Tcb1-s::Col48703 haplotype had been introgressed. The BAC sequencing reads were assembled with SPAdes v3.11.133. NODE_62, a contig that is 13,656 bp with coverage of 4029, was identified as having the Tcb1-f candidate gene. Whole genome sequencing reads from the two KO mutants were individually assembled with SPAdes v3.11.1 and BLASTed against NODE_62. Also, the mutant sequencing reads were mapped against NODE_62 using GSNAP34. Combining both approaches identified the hopscotch retrotransposon insertion in the tcb1-f(KO1)-mutant allele.
For phylogenetic analyses, alignments were made using the ClustalW algorithm in MegAlign (DNASTAR). The predicted mature PME enzymes and the Arabidopsis PME family members were taken from Markovic and Janecek, as were the subfamily designations29. Phylogenies were produced from these alignments using MrBayes v3.2.0 using default settings for amino acid analysis35. The MrBayes analysis was performed for 4,100,000 generations at which point the standard deviation of the split frequencies was below 0.004.
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
RNA-seq and genomic sequence data of Knockout lines and sequence reads of the BAC clone carrying Tcb1-f are available at NCBI BioProjects PRJNA528310 and PRJNA528983 and PRJNA532627. Coding and genomic sequence of Tcb1-f are available at GenBank MK789594 and MK789593. Data supporting the findings of this work are available within the paper and its Supplementary Information files. The source data underlying Figs. 2, 3, and 4 are provided as a Source Data file. Seed stocks and other data are available from the authors.
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The authors would like to thank Kathy Barton for the encouragement, insight, and support that kept this project alive, and to thank members of the Evans and Barton labs past and present for helpful discussions. We would also like to thank Beverly Oashgar for help with the screen for loss-of-function mutants and David Heller, Lance Cabalona, Clayton Coker, Amber Glowacki, and Hannah Vahldick for help growing plants and making crosses, and Jeffrey Yen for help isolating BAC clones. We would also like to thank Jeffrey Ross-Ibarra for help in calculating the divergence time between ZmPME3 and Tcb1-f. This work was supported by National Science Foundation Award number IOS-0951259 and by United States Department of Agriculture-National Research Initiative Competitive Grants Program Award number 35301-13314.
M.M.S.E. and J.L.K. are named as inventors on U.S. Patent No. 7,074,984: Cross-incompatibility traits from teosinte and their use in corn (J.L.K., Steven R. Gerrish, and M.M.S.E.). The remaining authors declare no competing interests.
Journal peer review information: Nature Communications thanks James Holland and other anonymous reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.
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Lu, Y., Hokin, S.A., Kermicle, J.L. et al. A pistil-expressed pectin methylesterase confers cross-incompatibility between strains of Zea mays. Nat Commun 10, 2304 (2019). https://doi.org/10.1038/s41467-019-10259-0
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