Squamous cell carcinomas (SCCs) are aggressive malignancies. Previous report demonstrated that master transcription factors (TFs) TP63 and SOX2 exhibited overlapping genomic occupancy in SCCs. However, functional consequence of their frequent co-localization at super-enhancers remains incompletely understood. Here, epigenomic profilings of different types of SCCs reveal that TP63 and SOX2 cooperatively and lineage-specifically regulate long non-coding RNA (lncRNA) CCAT1 expression, through activation of its super-enhancers and promoter. Silencing of CCAT1 substantially reduces cellular growth both in vitro and in vivo, phenotyping the effect of inhibiting either TP63 or SOX2. ChIRP analysis shows that CCAT1 forms a complex with TP63 and SOX2, which regulates EGFR expression by binding to the super-enhancers of EGFR, thereby activating both MEK/ERK1/2 and PI3K/AKT signaling pathways. These results together identify a SCC-specific DNA/RNA/protein complex which activates TP63/SOX2-CCAT1-EGFR cascade and promotes SCC tumorigenesis, advancing our understanding of transcription dysregulation in cancer biology mediated by master TFs and super-enhancers.
Squamous cell carcinomas (SCCs) are aggressive malignancies arising from squamous epithelium of various organs, such as esophagus, head and neck, lung and skin. Recently, large-scale molecular studies have characterized comprehensively both genomic and epigenomic (predominantly at the methylation level) alterations in different forms of SCCs1,2,3,4,5,6. However, these molecular profilings have not substantially improved clinical management of SCC patients, and no effective targeted regimens have been established for these cancers.
Although derived from diverse epithelial origins, SCCs have a number of unified genomic characteristics, with some being lineage-specific. The most notable SCC-specific genomic lesions target several transcription factors (TFs) with prominent functions in both healthy and neoplastic squamous cells, including SOX2, TP63, ZNF750, and NOTCH family genes. Specifically, SOX2 and TP63 are frequently co-amplified and overexpressed1,2,5, while the NOTCH family genes and ZNF750 exhibit recurrent loss-of-function mutations in SCCs4,5,7,8. These genetic alterations rarely occur in non-SCC cancers, highlighting their pathogenic significance in SCC biology.
Super-enhancers play prominent roles in driving expression of cell-type-specific genes through interacting with master TFs, co-factors, RNA polymerase II as well as non-coding RNAs9,10,11,12. We recently identified and characterized super-enhancer-associated genes with lineage-specific expression patterns in esophageal SCC (ESCC)13,14, including TP63 and SOX2. Previous investigations have shown that deletion of TP63 causes striking defects in epidermal development, highlighting its key role in the regulation of squamous cell differentiation and proliferation15,16,17. In squamous cancer cells, overexpression of either SOX2 or TP63 promotes proliferation and tumorigenesis1,18,19,20,21, suggesting oncogenic functions of these master TFs. Notably, Watanabe et al. (2014) showed that genomic occupancy of TP63 and SOX2 were significantly overlapping, and suggested functional co-operation between these two TFs in SCCs22. However, whether and how super-enhancers are under regulation by TP63 and SOX2, and its associated biological significance in SCCs remain unexplored.
Here, we perform epigenomic profilings to characterize the super-enhancer landscape in SCCs and investigate the importance of co-localization of TP63 and SOX2 at super-enhancer regions. Integrative analysis shows that TP63 and SOX2 co-bind to the promoter and super-enhancer regions of a long non-coding RNA (lncRNA), CCAT1, a novel SCC oncogenic lncRNA. Further mechanistic exploration demonstrates a complex interplay between CCAT1, TP63, and SOX2 in the transcriptional activation of EGFR, resulting in the hyper-activation of EGFR downstream pathways in SCC cells.
Super-enhancer regions are co-occupied by TP63 and SOX2 in SCC
Cancer cells acquire super-enhancers to drive expression of prominent oncogenes by recruiting a high density of TFs, coactivators, and noncoding RNAs9,12,23,24. To explore whether and how super-enhancers are regulated by SCC master TFs (TP63 and SOX2), we first performed chromatin immunoprecipitation sequencing (ChIP-seq) with antibodies against H3K27 acetylation (H3K27ac), TP63, and SOX2. Super-enhancer-associated genes were annotated (Fig. 1a, b and Supplementary Data 1-4). Consistent with our previous report, a numbered of well-defined SCC oncogenes had super-enhancers, (e.g. TP63, SOX2, EGFR, and MYC)13,20,21,22,25 in four ESCC cell lines. SCC-specific expression pattern was, as expected, observed with both TP63 and SOX2 (Supplementary Fig. 1). A direct interaction between TP63 and SOX2 in SCC was also verified by our immunoprecipitation (IP) results (Supplementary Fig. 2), confirming a previous report22.
Not surprisingly, the majority of TP63 and SOX2 ChIP-seq peaks were located at intergenic and intron regions (Fig. 1c and Supplementary Fig. 3). Importantly, almost half of TP63 binding peaks overlapped with SOX2 enriched loci (Fig. 1d, e). However, in embryonic stem cells, such overlapping genomic pattern of TP63 and SOX2 was absent22, suggesting their unique functional interplay in SCCs. We observed prominently enriched H3K27ac signals adjacent to both TP63 and SOX2 peaks (Fig. 1e), suggesting that transcriptional activation was associated with the binding of these two TFs.
To gain additional insights into the interactions between super-enhancers and TP63 and SOX2, we assigned TP63 and SOX2 ChIP-seq peaks to both super-enhancers and typical-enhancers. Notably, the co-occupancy of TP63/SOX2 was significantly enriched in super-enhancer-associated genes, relative to unique occupancy from either TFs (Fig. 1f left three columns). This significant enrichment of co-binding was specific to super-enhancer elements, as it was not observed in typical-enhancers (Fig. 1f right three columns). Moreover, super-enhancer-associated genes which were co-bound by TP63 and SOX2 consistently had higher mRNA levels relative to those unique-bound by either TP63 or SOX2 across all four SCC cell lines (Fig. 1g). These results together characterized the landscape of ESCC super-enhancers, and suggest that TP63 or SOX2 co-operatively activate a subset of these super-enhancers with a higher potency.
Identification of CCAT1 as a key target co-regulated by TP63 and SOX2 through a super-enhancer
To identify gene targets which are regulated by TP63/SOX2, we silenced either TP63 or SOX2 with shRNAs in SCC cell lines (Supplementary Fig. 4) and followed by whole-transcriptome sequencing (RNA-seq) (Fig. 2a, b and Supplementary Data 5-8). Given our earlier findings that TP63- and SOX2-occupied regions were strongly enriched for H3K27ac modification, we primarily focused on genes that were downregulated following the silencing of these two TFs. Gene Ontology (GO) analysis showed that downregulated genes (decreased greater than onefold relative to control) upon TP63 or SOX2 silencing were strongly enriched for cellular phenotypes important for cancer biology, including cell-cycle regulation, chromatin binding, and cell proliferation (Fig. 2a). To identify high-confident downstream targets co-regulated by TP63/SOX2, we further required that these transcripts were co-occupied by the two TFs based on our ChIP-seq data. As a result, we identified a total of 154 and 78 downregulated super-enhancer-associated transcripts upon knockdown of either TP63 or SOX2, respectively. Importantly, 52 of these transcripts were overlapped (P < 10−10, two-tailed Student t tests), suggesting a strong co-regulation of the down-stream targets between TP63 and SOX2. Among these 52 transcripts, ETV4 was experimentally validated as a common target of the two TFs22. Notably, two transcripts (CCAT1 and TXNRD1) consistently had reduced mRNA levels upon either TP63 or SOX2 knockdown (Fig. 2b) across all three ESCC cell lines.
CCAT1 (Colon cancer associated transcript-1) is a lncRNA, which was initially noted to be highly expressed in colon cancers26. The oncogenic property of CCAT1 was recently reported in several types of tumors, including cancers of the liver, gallbladder and stomach, as well as ESCC27,28,29,30. Notably, we also identified CCAT1 as a super-enhancer-associated gene, which was strongly expressed in ESCC (Fig. 1a). We validated that either TP63 or SOX2 knockdown caused significant reduction of the mRNA level of CCAT1, while overexpression either of TFs induced CCAT1 expression in SCC cell lines (Fig. 2c and Supplementary Fig. 5). However, expression of TP63 or SOX2 was not altered following silencing of CCAT1 (Supplementary Fig. 6), suggesting that CCAT1 is the downstream target of TP63 and SOX2 in SCCs.
Human CCAT1 gene is located at chr8q24.21, a recurrently amplified genomic region in SCCs. Therefore, we analyzed copy number alteration of CCAT1 in three major SCC types, ESCC, head and neck SCC (HNSCC) and lung SCC (LSCC), based on the data from The Cancer Genome Atlas Network (TCGA). As expected, in all SCC types, recurrent co-amplification of TP63 and SOX2 was detected (Fig. 2d). Importantly, although CCAT1 also exhibited genomic amplification (Fig. 2d), the level of CCAT1 mRNA was markedly higher in TP63/SOX2 co-amplified samples relative to CCAT1-amplified samples across different types of SCC tumors (Fig. 2e), suggesting that TP63/SOX2 co-regulation plays a more important role in driving CCAT1 expression than the amplification of CCAT1 DNA itself. Furthermore, the mRNA level of CCAT1 correlated significantly with that of TP63 and SOX2 in all SCC tumors (Fig. 2f). Given that TP63 and SOX2 are often overexpressed in SCC tumors compared with corresponding nonmalignant tissues, we next analyzed CCAT1 expression levels. Indeed, mRNA upregulation of CCAT1 in tumor samples relative to nonmalignant tissues was consistently observed in all types of SCCs (Fig. 2g). Together, these results identify super-enhancer-associated CCAT1 as a downstream target co-regulated by TP63 and SOX2, which are recurrently co-amplified in SCCs.
CCAT1 promotes SCC cell proliferation both in vitro and in vivo
To begin to probe the biological function of CCAT1 in SCCs, we procured a total of 28 SCC cell lines of ESCC, LSCC, HNSCC and cervical SCC (CSCC) (Supplementary Fig. 7a). A total of 12 of these cell lines with high CCAT1 expression representing each type of SCC were selected for loss-of-function assays (Supplementary Fig. 7a, b). Importantly, silencing of CCAT1 strongly impaired both cell viability and clonogenic capacity in all 12 SCC lines (Fig. 3a, b). We next ectopically overexpressed CCAT1 in both KYSE150 and KYSE510 cells, which had the lowest level of CCAT1 among all 13 lines (Supplementary Fig. 8a-c). Importantly, overexpression of CCAT1 significantly increased both the proliferation and colony growth of these two cell lines (Supplementary Fig. 8d, e). Moreover, CCAT1 knockdown in the xenograft assays resulted in marked reduction in both volume and mass of the tumors (Fig. 3c–e). These data showed a strong oncogenic potential of CCAT1 in SCC cells.
TP63 and SOX2 co-occupy at both the promoter and super-enhancers of CCAT1
To explore how CCAT1 was regulated by TP63 and SOX2, we analyzed ChIP-seq data of H3K27ac, TP63, and SOX2 generated from SCC cells, and compared them to those from other cells types, including embryonic stem cells and adenocarcinoma cells from various organs. The first observation was that the super-enhancers flanking CCAT1 (denoted by two red bars on top of Fig. 4a) were SCC-specific, since they were either undetectable or much weaker in adenocarcinoma cells of the esophagus (OE33, OE19, Flo-1), lung (A549 and H2009), and cervix (HeLa). Importantly, both CCAT1 promoter and super-enhancer regions [particularly constituents Enhancer 1 (E1) and Enhancer 2 (E2)] were co-occupied by TP63 and SOX2 in almost all SCC cell lines (except for TP63 at the promoter of JHU-029 cells, Fig. 4a). Again, this binding pattern was absent in non-SCC cells, suggesting the lineage-specific feature.
To validate the ChIP-seq results, ChIP-qPCR was performed to quantify the occupancy of H3K27ac, TP63, and SOX2, and their enrichment was confirmed at the promoter and super-enhancers (E1 and E2) of CCAT1 (Fig. 4b–d). We further performed luciferase reporter assays, and demonstrated that the reporter activity was prominently increased upon transfection of either CCAT1 promoter or enhancer (E1 or E2) (Fig. 4e and Supplementary Fig. 9). Importantly, silencing of TP63 or SOX2 potently inhibited this reporter activity (Fig. 4e and Supplementary Fig. 9). These data demonstrate that TP63 and SOX2 co-occupy the promoter and super-enhancers of CCAT1, thereby activating its transcription in SCC cells in a lineage-specific manner.
CCAT1 activates both MEK/ERK1/2 and PI3K/AKT signaling pathways
To characterize the mechanisms underlying CCAT1-mediated cellular effects, RNA-Seq was first performed upon silencing of CCAT1 in different SCC cells. Interestingly, gene set enrichment analysis (GSEA) showed that super-enhancer-associated genes, but not typical-enhancer-associated genes, were preferentially downregulated upon silencing of CCAT1 (Fig. 5a), suggesting that silencing of CCAT1 disproportionately downregulated super-enhancer-mediated transcription. GO analysis revealed that these downregulated genes were enriched for processes involved in cancer biology, including cell proliferation, growth, and migration (Fig. 5b). This is in agreement with our findings that CCAT1 was required for SCC cell viability. Indeed, silencing of CCAT1 led to downregulation of many genes involved in cancer cell proliferation, survival and metastasis, such as EGFR, CDK4, YES1, PAK4, and HMGA1 (Fig. 5c and Supplementary Data 9). Notably, both EGFR and PAK4 were also associated with super-enhancers in SCC cells (Fig. 1a)13. Interestingly, genes involved in DNA and protein interactions (e.g., nucleotide binding, protein complex biogenesis and transcription cofactor binding) were also downregulated following CCAT1 knockdown (Fig. 5b), implying that CCAT1 might regulate the interaction of macromolecules.
To complement the investigations at RNA level, the Human Phospho-kinase array was utilized to detect changes of phosphorylated proteins associated with silencing of CCAT1. Notably, CCAT1 knockdown cells had reduced phosphorylation levels of a number of key molecules mediating the mitogen-activated protein kinase kinase/extracellular signal-regulated kinase 1/2 (MEK/ERK1/2) and phosphatidylinositol 3-kinase (PI3K/AKT) signaling pathways, including AKT, MEK1/2, ERK1/2, and p38 (MAPK) (Fig. 5d). Moreover, both upstream (EGFR) and downstream proteins (β-catenin, AMPK, WNK, and CREB) of these two pathways exhibited concordant reductions in their phosphorylation levels. Given the critical roles of these pathways in cancer biology, western blotting was performed to validate these changes. Indeed, the phosphorylation levels of all of the tested signaling molecules were consistently reduced in CCAT1-silenced SCC cells and xenografts compared to controls (Fig. 5e and Supplementary Fig. 10). Importantly, all of these reductions were also evident in either TP63 or SOX2 silenced cells (Fig. 5e), strongly supporting our earlier findings that CCAT1 was the downstream target of these two master TFs.
Integration of the results of both RNA-seq analysis and phospho-kinase array, drew attention to EGFR, a super-enhancer-associated oncogene, which exhibited consistent decreases in mRNA, protein and phosphorylation levels following CCAT1 depletion (Fig. 5f, g). Importantly, abundances of mRNA, total protein as well as the phosphorylation of EGFR were all markedly decreased upon silencing of either TP63, SOX2 or CCAT1 (Fig. 5f, g).
Moreover, the phosphorylation levels of key signaling mediators were restored upon overexpression of EGFR in CCAT1-silenced cells. In addition, overexpression of EGFR rescued significantly the decreased proliferation and colony growth of SCC cells induced by CCAT1 knockdown (Supplementary Fig. 11). These results characterized a novel regulatory cascade involving TP63/SOX2-CCAT1-EGFR, which activates both MEK/ERK1/2 and PI3K/AKT signaling pathways in SCC cells.
CCAT1 recruits both TP63 and SOX2 to the super-enhancers of EGFR to promote its transcription
To elucidate the mechanisms underlying TP63/SOX2-CCAT1-EGFR regulation, ChIP-seq data was initially interrogated. Notably, flanking EGFR were two super-enhancers (denoted by red bars on top of Fig. 6a), and TP63/SOX2 occupied both of them (shadowed regions containing E1, E2, and E3 enhancer) across all SCC cell lines (Fig. 6a). Again, these two super-enhancers were SCC-specific, being either not present or much weaker in LUAD, CAC and EAC cells. To evaluate the activity of these super-enhancer constituents, a series of luciferase reporter assay were performed. Each constituent (E1, E2, and E3) alone was capable of elevating the reporter activity (Fig. 6b and Supplementary Fig. 12). Importantly, all of these enhanced reporter activities were strongly reduced by silencing either TP63, SOX2, or CCAT1 (Fig. 6b and Supplementary Fig. 12), suggesting that the EGFR super-enhancers activity are modulated by TP63, SOX2, and CCAT1.
Rescue assays were next carried out to determine the mechanistic relationship between TP63/SOX2 and CCAT1 in the regulation of the EGFR super-enhancers. As expected, silencing of either TP63 or SOX2 reduced EGFR expression (Figs. 5g and 6c). Importantly, the reduction was not rescued by overexpression of CCAT1 (Fig. 6c and Supplementary Fig. 13), suggesting that CCAT1 cannot activate the EGFR enhancer independent of TP63 and SOX2. Notably, the abundance of occupancy of TP63 and SOX2 at super-enhancers (E1, E2 and E3) of EGFR substantially decreased after CCAT1 knockdown (Fig. 6d and Supplementary Fig. 14). These data suggest that the co-binding affinity of TP63/SOX2 at EGFR super-enhancers was maintained or reinforced by CCAT1 RNA. However, downregulation of CCAT1 did not affect the binding between TP63 and SOX2, suggesting that this protein-protein interaction is independent of CCAT1 (Supplementary Fig. 15). Given the physical association between TP63 and SOX2, these results also imply that TP63, SOX2, and CCAT1 might form a complex at these enhancers.
This hypothesis was first tested by RNA immunoprecipitation (RIP) pulling down the TP63- or SOX2-containing complex using either TP63 or SOX2 antibody. RT-PCR results showed that CCAT1 RNA strongly associated with TP63 and SOX2, but not with IgG antibody (Fig. 6e). To complement the RIP assay, chromatin isolation by RNA purification (ChIRP) was conducted. We designed a total of 13 probes tiling the full-length of CCAT1 RNA, and they were separated into odd and even pools31,32. Following IP, CCAT1-bound proteins and DNAs were detected by western blotting and qPCR assays, respectively. CCAT1 probes successfully retrieved ~60% of cellular CCAT1 RNA but minimal GAPDH RNA (negative control) (Supplementary Fig. 16a). On the other hand, probes tiling the Laz gene did not retrieve either CCAT1 or GAPDH RNA (Supplementary Fig. 16a). These results validated both the specificity and efficiency of CCAT1 ChIRP probes. Importantly, the interactions between CCAT1 RNA with both TP63 and SOX2 proteins were detected by western blotting in two different SCC cell lines (Fig. 6f), supporting the existence of a complex containing CCAT1 RNA, TP63, and SOX2 proteins.
We next examined DNA molecules, which were immunoprecipitated by CCAT1 ChIRP probes. Importantly, the EGFR super-enhancer constituents (E1, E2 and E3) were strongly enriched in the CCAT1-containing complex (Fig. 6g, Supplementary Figs. 1, 6b and 1, 6c). Moreover, knockdown of either TP63 or SOX2 markedly reduced the occupancy of CCAT1 in all three enhancer regions in comparison with control shRNA group (Supplementary Fig. 17). Taken together, the data demonstrate that a protein/nucleotide complex containing TP63/SOX2/CCAT1 occupies the super-enhancers of EGFR and promotes the transcription of EGFR in SCC cells.
TP63 and SOX2 are master TFs in SCC cells, which are also associated with super-enhancers themselves. However, whether and how super-enhancers are regulated by TP63 and SOX2 remains to be elucidated. In this study, we first noted that co-localization of TP63/SOX2 at super-enhancers occurred more frequently than unique occupancy by either TFs. Furthermore, this enrichment of co-binding was unique to super-enhancers, since it was not observed in typical-enhancer elements. Importantly, transcripts co-occupied by both TFs had higher expression relative to those bound by either TFs, suggesting stronger functional cooperation of TP63/SOX2 at super-enhancer elements.
Integrative analysis of both RNA-seq and ChIP-seq identified 52 transcripts as super-enhancer transcriptional targets directly co-occupied by both TP63 and SOX2. Among them, CCAT1 and TXNRD1 showed consistent changes across all three SCC cell lines examined. CCAT1 was detected to be distributed both in the nucleus and cytoplasm29,33. Previous reports studying gastric, colon and live cancers demonstrated that c-MYC could directly bind to the promoter region of CCAT1 to enhance expression of this lncRNA, facilitating tumor progression34,35,36. Here, we observed that TP63 and SOX2 co-bound to the promoter and super-enhancer regions of CCAT1 and contributed to its transcription activity specifically in SCCs, but not other cancer types. These results indicate tissue-specific regulations of CCAT1 transcription, which is consistent with the lineage-specific nature of TP63 in SCC cells.
Functionally, our results characterized CCAT1 as a prominent oncogenic lncRNA in all four types of SCCs tested. To decipher the mechanisms responsible for the actions of CCAT1, both RNA-seq and phospho-kinase array were performed which identified EGFR as an important target of CCAT1. Nuclear lncRNAs have been observed to be involved in chromatin interactions, transcriptional regulation, and RNA processing by binding with DNA, RNA, and TFs; cytoplasmic lncRNAs can modulate stability or translation of transcripts and influence cellular signaling cascades37,38,39,40,41. For example, HOTAIR directly binds androgen receptor (AR), promotes AR-dependent transcriptional network and drives castration-resistant prostate cancer42. Notably, in the regulation of neurogenesis, SOX2 was found to interact with lncRNA-RMST, forming a protein/RNA complex binding at the promoter regions of several neurogenic TFs, including SP8, HEY1, NEUROG2, and DLX1. SOX2 occupancy at these promoters was reduced upon RMST knockdown, resulting in downregulation of the expression of these neurogenic TFs43. Similarly, using ChIP-qPCR and ChIRP-qPCR, we showed that both CCAT1 and SOX2 co-occupied the super-enhancer regions of EGFR in SCCs cells, and that CCAT1 was required for SOX2 binding (Fig. 6d, g). To the best of our knowledge, lncRNAs interacting with TP63 have not been identified previously. Here we showed the interaction of CCAT1 with TP63 and, together with SOX2, they co-bound EGFR super-enhancers and promoted their activities.
In colorectal cancer cells, a study noted that CCAT1 was located within a super-enhancer and interacted with CTCF protein to maintain chromatin looping between the MYC promoter and its enhancers, resulting in elevated expression of MYC33. In SCC cells, the mechanism by which CCAT1 regulates EGFR transcription appears similar to a certain degree, i.e., forming a protein/DNA/RNA complex within cis-regulatory regions. These results highlight CCAT1 as an oncogenic enhancer RNA (eRNA) with unique functions in SCC cells44, as it regulates transcription via interacting with both master TFs (TP63 and SOX2) and DNA molecules (super-enhancer loci of EGFR). Occupancy of TP63/SOX2/CCAT1 complex at EGFR super-enhancers induced high transcription of the gene, which activates both MEK/ERK1/2 and PI3K/AKT signaling pathways, driving SCC cells proliferation and survival45,46. Interestingly, in gastric cancer cells CCAT1 modulated cancer cell proliferation, migration and invasion also by regulating the ERK/MAPK pathway47, congruent with our findings in SCC cells.
In summary, we provide compelling evidence to highlight a closely cooperative machinery between super-enhancers, lncRNA and master TFs that contribute to SCCs malignancy. SCC master TFs, TP63, and SOX2, transcriptionally activate CCAT1 RNA through direct co-occupying its promoter and super-enhancer elements. CCAT1 in turn recruits both TP63 and SOX2 and forms a protein/RNA complex, co-localizing at EGFR super-enhancers to activate its transcription. These complex regulations result in the high expression of both CCAT1 and EGFR, promoting SCC tumor progression through activating downstream MEK/ERK1/2 and PI3K/AKT signalings (Fig. 7).
Human cell lines
KYSE cell line series were provided by Dr Y Shimada (Kyoto University, Japan), and TE-5 and TT cells were provided by Dr Koji Kono (Cancer Science Institute of Singapore, Singapore). HNSCC cell lines (UMSCC1 and 93UV147) were kindly provided by Dr Timothy Chan (Memorial Sloan Kettering Cancer Center). LSCC cell lines (SK-MES-1, Calu-1, ChagoK1, H520, H2170 and H226) kindly provided by Dr GOH Boon Cher (Cancer Science Institute of Singapore). ESCC, LSCC, HNSCC, CSCC Cells were maintained at 37 °C in 5% CO2 in either RPMI-1640 medium or DMEM supplemented with 10% FBS, penicillin, and streptomycin. All these cells were recently authenticated by STR analysis13,48.
Cell proliferation and colony formation assays
Cells were seeded into 96-well plates (2000–5000 cells/well) in quintuplicate. Cell proliferation was measured using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) staining. For colony formation assay, cells were seeded into six-well plates (100–500 cells per well) and cultured for 2–3 weeks. Resulting colonies were calculated following 1% crystal violet staining.
RNA extraction, cDNA synthesis, and quantitative PCR
Total RNA was extracted with RNeasy Mini kit (QIAGEN) and cDNA was obtained from the total RNA using EvoScript Universal cDNA Master (Roche). Quantitative PCR (qRT-PCR) was conducted with PrecisionTMFAST Mastermix (Precision, Precision-LR). GAPDH was used for normalization. Primers used in the study were listed in Supplementary Table 1.
RNA-seq data analysis
The 100 bp paired end reads were aligned to hg19 Ensemble (V82) transcriptome using Kallisto pseudo aligner49. Transcript level abundances and counts were summarized to gene level using tximport Bioconductor package50. Differential gene expression analysis was performed using DESeq/DESeq251,52. RNA-Seq data for KYSE70 and TT cell lines were downloaded from GEO (GSE47058) and processed in a similar way to avoid bias due to differences in analysis protocols. All differentially expressed genes (adjusted P value < 0.1) were used for Gene Ontology analysis using goseq Bioconductor package53. For GSEA analysis, we used all expressed genes with mean FPKM values > 0.5.
Western blotting and IP
Cells were lysed with in RIPA lysis and extraction buffer (Thermo Fisher Scientific), supplemented with proteinase inhibitor cocktail, phosphatase inhibitor cocktail (Roche) for 30 min on ice. Protein concentrations were determined with Bio-Rad Protein Assay Kit (Bio-Rad) according to the instruction. Western blotting was performed using SDS-PAGE followed by transfer to nitrocellulose membrane (Bio-Rad). Primary antibody was incubated overnight in cold room. Secondary antibody was incubated for 1–2 h at room temperature.
For IP, 500 µg whole-cell lysate (for each experiment) was incubated with either indicated antibody or IgG on the rotary agitation overnight in cold room. The immunoprecipitates were then incubated with Dynabeads protein A/G (ThermoFisher Scientific) for 4 h in cold room, followed by purification and Western blotting analysis using indicated antibodies (Supplementary Fig. 15). Primary antibodies used were listed in Supplementary Table 2. Secondary antibodies were purchased from GE Healthcare SciMed.
Plasmid transfection and lentiviral production
Lentiviral cloning vector pLKO.1-TRC (Plasmid #10878), pcDNA3.1-ΔNP63alpha-Flag (Plasmid # 26979), and pcDNA3.3-SOX2 (Plasmid #26817) plasmids were purchased from Addgene. The double-stranded oligonucleotide shRNAs were cloned into the AgeI/EcoRI sites of the pLKO.1-TRC lentiviral vector. Plasmid transfection, lentivirus production and lentivirus infection in cell lines were described previously13,54. shRNA target sequences were listed in Supplementary Table 3. As TP63 has two isoforms-TAp63 and ΔNp63, we designed two shRNAs, with shRNA-1 targeting all TP63 isoforms and shRNA-2 specifically targeting only ΔNp63α isoform. Both of them were efficient in knocking down TP63 mRNA and protein expression (Supplementary Fig. 4).
Animal studies were performed in accordance with protocols approved by the ethical regulations of Institutional Animal Care and Use Committee (IACUC) of National University of Singapore. Xenograft models were established by subcutaneous injecting of 1 × 106 TE5-Scramble or TE5-CCAT1 silencing cell lines (TE5-shCCAT1-1 or TE5-shCCAT1-2) into the flank of recipient NOD-SCID Gamma (NSG) mice (six weeks old, six mice). Mice general behaviors were monitored and the tumor volume was measured every 5 days for a total 20 days. At the end of the experiments, mice were sacrificed and the tumor tissues were collected for growth analysis.
Luciferase reporter assay
Luciferase reporter vectors were purchased from Promega. The cDNA fragments of each promoter or enhancer regions containing were amplified using PCR and then inserted into pGL3-Promoter or pGL3-Enhancer vectors. The constructs with correct sequences were used for the transfection and a Renilla luciferase plasmid was co-transfected as a normalization control. The luciferase assays were carried out with Dual-Luciferase Reporter Assay System (Promega) and reporter activity was measured by Luminometer (Promega). The primers used for the amplification of each region were listed in Supplementary Table 4.
Human phospho-kinase array
Human phospho-kinase array was performed according to the manual instructions (R & D Systems). Briefly, cell lysate were diluted into the desired quantity (1 µg/µl, 200–300 µg per sample for one experiment), incubated with membranes (A and B parts) overnight at 4 °C on a rocking platform. The membranes were then incubated with detection antibody cocktail A or B for 2 h following by Streptavidin-HRP for another 30 min at room temperature on a rocking platform shaker. Signal detection applied the Chemi Reagent Mix and exposed to film.
To study interactions of TP63 or SOX2 protein with CCAT1, RIP was performed using the published protocols55. Briefly, cell lysates were firstly incubated with Dynabeads protein A/G to remove non-specific binding. The precleared lysate was incubated with indicated antibody or the same type of IgG antibody at 4 °C overnight. Dynabeads were then added to immunoprecipitate antibody-antigen complexes at 4 °C for 4 h and the unbound proteins were washed off. RNA was then separated from the beads-antibody-RNA complexes for reverse transcription followed by PCR analysis.
ChIRP analysis was performed according to the published protocols31,32. CCAT1 anti-sense DNA probes with BiotinTEG at 3-prime end were designed using online probe designer (singlemoleculefish.com) and produced by Biosearch Technologies. Cells were collected and subjected to crosslink with 1% glutaraldehyde. The crosslinked cells were lysed and sheared into 100–500 bp DNA fragments by sonicating in a 4 °C water bath at highest setting with 30 s ON, 45 s OFF pulse intervals. CCAT1 biotinylated probes were separated into odd and even two pools to hybridize with RNA at 37 °C for 4 h with shaking. LacZ probes were used as a negative control. qRT-PCR was performed with RNA samples to confirm lncRNA-CCAT1 retrieval. Interaction of CCAT1 with DNA and proteins were examined by qPCR and Western blotting analysis. Probes used in this study are listed in Supplementary Table 5.
ChIP sequencing and analysis
Cells were crosslinked with 1% formaldehyde solution and neutralized by 1.25 M glycine. Crosslinked cells were then lysed and sonicated with Bioruptor (Diagenode). Sonicated chromatin was precleared with Dynabeads protein A/G then incubated with indicated antibody for overnight at 4 °C and with beads for additional 2 h. DNA was eluted from immunoprecipitate complexes, reverse crosslinked and purified with QIAquick PCR spin kit (QIAGEN). High quality ChIP DNA were sequenced on Illumina HiSeq2000. ChIP-qPCR was performed to verify ChIP-seq results13,54. Gene desert (chr11: 127,277,673–127,322,674) serves as a negative control56. Primers for qPCR were shown in Supplementary Table 1. For ChIP-seq analysis, 50 bp single-end ChIP-seq reads were aligned to reference human genome (hg19) using bowtie short-read aligner, with alignment parameters –e70 –k2 –m2 –n2 –best –strata57. PCR duplicates were marked with picard MarkDuplicates and removed from further analysis. Peaks were identified using MACS2 peak caller with parameters –bdg –SPMR –nomodel –extsize 200 –q 0.0158. Bedgraph files generated with MACS2 were later converted bigwig using ucsc bedGraphToBigWig tool.
ChIP-seq data for TP63 and SOX2 in KYSE70 and TT cell lines were downloaded from GSE46837 and processed uniformly. TP63 and SOX2 peaks from all four cell lines were merged to generate a consensus peak sets. Overlapping SOX2 and TP63 peaks were considered as co-occupied regions. SOX2 or TP63 peaks with no nearby co-occupancy from the other TF within a distance of 1 kb were considered as unique peaks. In Fig. 1e, ChIP-seq signals from bigwig files were extracted using bwtool (summary and matrix subcommands) and manually plotted in R using base graphics or ComplexHeatmap Bioconductor package59,60. Super-enhancer-associated genes were identified using ROSE framework. All other test statistics were performed in R (VN. 3.3.0).
For comparisons of continuous variables between groups, two-tailed Student t tests were used. Data were shown as the mean ± SD. The values at P < 0.05 (*), P < 0.01 (**), and P < 0.001 (***) were considered statistically significant. The details of statistical analysis were presented in figure legends. Diagrams were created by GraphPad Prism software.
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We thank Dr. Chen Ling-Ling (Institute of Biochemistry and Cell Biology, China) for sharing the pcDNA3-CCAT1 plasmid. This research is supported by the National Research Foundation Singapore under its Singapore Translational Research (STaR) Investigator Award (NMRC/STaR/0021/2014) and administered by the Singapore Ministry of Health’s National Medical Research Council (NMRC), the NMRC Centre Grant awarded to National University Cancer Institute of Singapore, the National Research Foundation Singapore and the Singapore Ministry of Education under its Research Centers of Excellence initiatives. D-C.L is supported by the DeGregorio Family Foundation, the Price Family Foundation as well as Samuel Oschin Comprehensive Cancer Institute (SOCCI) at Cedars-Sinai Medical Center through the Translational Pipeline Discovery Fund. This research is additionally supported by National Natural Science Foundation of China (81672786 and 81520108023 to M.-R.W., 81871921 to J.-J.X.) and by the RNA Biology Center at the Cancer Science Institute of Singapore, NUS, as part of funding under the Singapore Ministry of Education’s Tier 3 grants, grant number MOE2014-T3-1-006.
The authors declare no competing interests.
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Jiang, Y., Jiang, YY., Xie, JJ. et al. Co-activation of super-enhancer-driven CCAT1 by TP63 and SOX2 promotes squamous cancer progression. Nat Commun 9, 3619 (2018). https://doi.org/10.1038/s41467-018-06081-9
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