Honeybee caste development is nutritionally regulated by royal jelly (RJ). Major royal jelly protein 1 (MRJP1), the most abundant glycoprotein among soluble royal jelly proteins, plays pivotal roles in honeybee nutrition and larvae development, and exhibits broad pharmacological activities in humans. However, its structure has long remained unknown. Herein, we identify and report a 16-molecule architecture of native MRJP1 oligomer containing four MRJP1, four apisimin, and eight unanticipated 24-methylenecholesterol molecules at 2.65 Å resolution. MRJP1 has a unique six-bladed β-propeller fold with three disulfide bonds, and it interacts with apisimin mainly by hydrophobic interaction. Every four 24-methylenecholesterol molecules are packaged by two MRJP1 and two apisimin molecules. This assembly dimerizes to form an H-shaped MRJP14-apisimin4-24-methylenecholesterol8 complex via apisimin in a conserved and pH-dependent fashion. Our findings offer a structural basis for understanding the pharmacological effects of MRJPs and 24-methylenecholesterol, and provide insights into their unique physiological roles in bees.
Royal jelly (RJ) is mixture of natural products secreted from the hypopharyngeal and mandibular glands of nurse honeybees (Apis mellifera)1. RJ is essential for queen reproduction and larvae development in honeybee colonies, and is now widely used in human health and beauty products. Castes are nutritionally regulated, and only larvae exclusively fed on RJ develop into queens2,3,4. Intriguingly, queen honeybees have an approximately twofold larger body size and tenfold longer lifespan than worker bees of the same genotype.
RJ contains 12–15% protein, of which 82–90% are members of the major royal jelly protein family (MRJPs) that share high homology, and are designated MRJP1−95,6,7. MRJPs have a common evolutionary origin with the Yellow protein family from insects and some bacteria8. MRJP1 is the most important and abundant glycoprotein in RJ and in bee bread, accounting for 48% of water-soluble RJ proteins1, and is also the most prominent colony-specific honeybee protein9. Besides their nutritional role, MRJP1 is a key factor responsible for honeybee development10,11,12. Furthermore, MRJP1 plays important roles in bee brain functions such as learning, memory, and social behaviour9,13. MRJP1 also exhibits a broad range of pharmacological activities in human health, such as promoting cell growth and wound healing14,15,16,17,18,19, broad-spectrum antibacterial and antifungal activities20,21, hypocholesterolemic effects22, antitumor activity23, vasodilative and anti-hypertension activity24,25, and immune enhancement26,27.
Soluble MRJP1 is present in both monomeric and oligomeric forms in RJ1. It exists predominantly as an oligomer in physiological conditions2,28,29. In particular, the MRJP1 oligomer (also called apalbumin) has significantly higher heat resistance and longer storage stability than the monomeric form30. This underscores the significance of the oligomer when studying the biological properties of MRJP1. Interestingly, the MRJP1 oligomer reportedly comprises both MRJP1 and apisimin. Apisimin is a serine-valine-rich peptide exclusively found in honeybee and has the tendency to form oligomers31. However, the composition, molecular weight, and binding stoichiometry of the MRJP1 oligomer remain controversial16,28,31,32. The molecular weight of the MRJP1 oligomer has been estimated to be 280 kDa2, 290 kDa28, 350 kDa17,33, 420 kDa1, or 450 kDa10. Moreover, no structures of the intriguing MRJP family members have been resolved at the atomic level5, which hampers rational understanding and drug applications.
Herein, we identify the components of the large MRJP1 oligomer, and determined the structure at 2.65 Å. 24-methylenecholesterol is unexpectedly found in it. To the best of our knowledge, no structures of MRJP family member proteins, apisimin, and 24-methylenecholesterol are reported previously. The overall architecture of MRJP14-apisimin4-24-methylenecholesterol8 reveals a quaternary structure consisting of a dimer of dimers, in which eight 24-methylenecholesterol molecules are packaged. Moreover, our biochemical, biophysical, and crystal structure analyses demonstrate that the assembly states of MRJP1 oligomers are highly conserved and pH-dependent. Interaction between two apisimin proceeds via a mode consisting mostly of hydrophobic contacts. Taken together, our findings provide a structural basis for a better understanding of the unique physiological roles of MRJP family proteins and 24-methylenecholesterol in bees and their pharmacological effects in humans.
Structure determination of the MRJP1 oligomer
Previous studies indicated that MRJP1 exhibits a relatively high degree of disorder32. Various constructs and fusion tags have been tested to express MRJP1 in E. coli and yeast systems. However, it was difficult to obtain sufficient amounts of soluble recombinant MRJP1 for X-ray crystallographic analysis. We therefore attempted to purify native MRJP1 from RJ. Using ultrafiltration, and ion-exchange and gel filtration chromatography steps, both the MRJP1 monomer and oligomer were purified to high homogeneity (Supplementary Fig. 1A, B). To further confirm the purified MRJP1 proteins, matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) and liquid chromatography (LC–MS/MS) were performed. For the oligomer, molecular mass analysis by MALDI-TOF MS identified the digested peptides as MRJP1 following database searches, with 84% coverage, and LC–MS/MS spectra further confirmed the peptide sequences (Supplementary Fig. 1C−F). The identity of the MRJP1 monomer was similarly confirmed by MS.
During initial screening, only the native MRJP1 oligomer successfully yielded crystals (Supplementary Fig. 2A). But the small needle-like crystals did not grow large, and the diffraction quality was poor, even after extensive optimisation of the crystallisation conditions using additives, detergents, and seeding (Supplementary Fig. 2B). Therefore, several protein treatments, such as protease digestion, lysine methylation34, and deglycosylation were performed. Lysine methylation increased the size of crystals (Supplementary Fig. 2C), but the diffraction resolution remained low. Interestingly, the native MRJP1 oligomer was only partly deglycosylated by peptide:N-glycanase (PNGase) F under mild conditions (Supplementary Fig. 3A), and several crystal forms were obtained after deglycosylation of MRJP1 oligomers (Supplementary Fig. 2D−F). Fortunately, high-quality diffraction data were eventually obtained using cuboid crystals (Supplementary Fig. 4A, B).
Because, the sequence homology between MRJP1 and any other protein of known three-dimensional structure was <17%, the structure were determined by combining multiple isomorphous replacement and molecular replacement methods, and refined to a resolution of 2.65 Å in space group of H32, with an Rwork of 23.9% and an Rfree of 27.5% (Table 1). The MRJP1 molecule possesses two orthogonal twofold axes, as confirmed by self-rotation analysis (Fig. 1a and Supplementary Fig. 4C).
Apisimin-dependent dimerisation of dimers MRJP1 structure
The asymmetric unit contains two MRJP1, two apisimin, four unexpected 24-methylenecholesterol (ostreasterol, Osl), and 144 water molecules. The electron density maps clearly showed electron densities for bound apisimin (Supplementary Fig. 5B). Moreover, the composite simulated-annealing mFo—DFc omit electron density map further confirmed the presence of apisimin (Fig. 1b). For both MRJP1 and apisimin, mature proteins were detected without N-terminal signal peptides. For MRJP1 molecules, protomer A spans residues 23–432 and protomer B spans residues 28–432 with N-acetylglucosamine linked to Asn144. Residues 197–199 and 290–291 in protomer A, residues 117, 120, 172–173, 183, 192–194, 196−199, 219–220, 242, 245–247, 284−288, 291−294, and 389–391 in protomer B could not be modelled due to disorder (Supplementary Fig. 6A), which may indicate conformational flexibility of these residues. For two apisimin molecules, protomer C spans residues 37–77 and protomer D spans residues 36–78. The two protomers of MRJP1 and apisimin are almost identical, with an overall root-mean-square deviation (RMSD) for all Cα atoms of 0.78 Å and 0.93 Å, respectively (Supplementary Fig. 6A, B). Details of the data collection and processing statistics are shown in Table 1.
The complete MRJP1-apisimin-24-methylenecholesterol ternary structure is obtained by applying a twofold symmetry operation. The overall complex adopts an H-shaped structure with approximate height, width, and length of 100 × 50 × 115 Å, respectively. It has an apisimin-dependent dimerisation of MRJP1 dimers quaternary structure, comprising 16 molecules, including four MRJP1, four apisimin, and eight 24-methylenecholesterol molecules (Supplementary movie 1).
The MRJP1 oligomer contains 24-methylenecholesterol
During refinement of the MRJP1 oligomer structure, we noticed four large electron density features located between two apisimin molecules in the asymmetric unit (Supplementary Fig. 5B), in which Osl fitted well. Moreover, the composite simulated-annealing mFo—DFc omit electron density map further confirmed the presence of Osl (Fig. 1c). Osl was previously reported to be present in RJ and honeybees35,36. Thus, to exclude any possible contamination from RJ directly, we first purified the MRJP1 oligomer to high homogeneity by ultrafiltration, ion-exchange and gel filtration chromatography steps. The unknown small molecule was then released from the purified MRJP1 oligomer by adding 2M NaOH and extracted with n-hexane. Three different methods were used to identify the small molecule, namely electron ionization gas chromatography–mass spectrometry (EI-GC/MS), 1H, and 13C NMR (Supplementary Fig. 7), and the results matched perfectly with those of the Osl standard/ reference compound37, confirming its identity.
Due to the existence of non-crystallographic twofold symmetry, the positions of the four Osl molecules could be divided into inner and outer types (Supplementary Fig. 6E). Each of the inner Osl molecules contact two MRJP1, one apisimin, and the other three Osl molecules in the asymmetric unit (Supplementary Fig. 6F), while each of the outer Osl molecules only interact with two apisimin molecules and the other three Osl molecules.
Disulfide-bond stabilised six-bladed β-propeller fold
MRJP1 is folded into a unique six-bladed β-propeller shape containing 31 β-strands and 11 α-helices with a central channel (Fig. 2). The protein fold is similar to that of LJM11 from sand fly Lutzomyia longipalpiss38, the template structure used for molecular replacement (Fig. 3b). Each blade consists of a four-stranded β-sheet, and the fourth β-strand of each blade connects with the first β-strand of the subsequent one to form a bucket. The wall of the central channel consists of a parallel arrangement of the first β-strands from each blade (Fig. 2). A series of large loops, β-strands, and α-helices are in the bucket periphery. The temperature factors of these peripheral regions are high, suggesting they are flexible (Supplementary Fig. 6C). A DALI search39 of the MRJP1 structure identified an uncharacterised protein, Ava_4197 from Anabaena variabilis (PDB ID 2qe8, RMSD = 2.4 Å, Z = 34.6) as the closest structural homolog. Structural alignment between MRJP1, Ava_4197, and LJM11 indicated that the position of the core six-bladed β-propeller was similar, while the peripheral bucket was somewhat more variable in terms of the number and position of α-helices, β-strands, and loops (Fig. 3a, b). Interestingly, the C-terminus of MRJP1, which interacts with a neighbouring MRJP1, is much longer than those of Ava_4197 and LJM11 (Fig. 3a, b, and Supplementary Fig. 8A). Moreover, very low sequence conservation is observed between MRJP1 and other Yellow protein family members (Supplementary Fig. 8A), suggesting MRJP1 might function differently from other Yellow proteins.
Three disulfide bonds (Cys132–Cys195, Cys118–Cys150, and Cys329–Cys416) were observed in MRJP1, with the last one stabilising the C-terminal portion (Supplementary Fig. 6A). All cysteine residues in the last two disulfide bonds of MRJP1 are conserved in homologous members of the MRJP family (Supplementary Fig. 8B), suggesting that the overall structure might be conserved, and both disulfide bonds are essential for structural stability. By contrast, the Cys132–Cys195 disulfide between two blades of MRJP1 is not conserved in other MRJPs, and the most substantial differences are observed in the C-terminal region (Supplementary Fig. 8B). In addition to the very low sequence homology between MRJP1 and other Yellow family members, the Cys118–Cys150 disulfide bond is not present in LJM1138, and no disulfide bond was found in Ava_4197, highlighting the divergence of MRJPs from other Yellow protein family members. Overall, the marked differences in disulfide bonds and other sequence features may indicate that MRJP1 is functionally different from its homologs.
The apisimin structure and interaction mode
All residues in the determined apisimin structure are conserved across different species (Supplementary Fig. 8C), indicating that the overall structure may be similar. Each apisimin molecule in the asymmetric unit contains two α-helices connected by a loop, with two hydrogen bonds and a large hydrophobic zone between the two α-helices, giving apisimin a relatively stable 47° oblique structure (Supplementary Fig. 6B). Consistently, both termini appear to be more flexible than the central region, as reflected by their higher temperature factors (Supplementary Fig. 6D).
A DALI search of the apisimin structure identified the chaperone GrpE (PDB 3a6m, RMSD = 4.2 Å, Z = 5.3)40 as the closest structural homolog (Fig. 3c). However, the sequence similarity is <10%, indicating low homology. Additionally, only interaction between apisimin protomers from different MRJP1 dimers is observed, and apisimin molecules in the same MRJP1 dimer do not interact. By contrast, in GrpE, interactions occur between two protomers from the same GrpE dimer (Fig. 3c). Furthermore, only the C-terminal α-helix of apisimin is involved in interactions, and hence forming the MRJP1 oligomer (MRJP14-apisimin4-Osl8), which is clearly distinct from GrpE in which both helices are involved (Fig. 3c). Moreover, the interface area of each apisimin is only 509 Å2, which is significantly lower than the 3051 Å2 for each GrpE molecule, as calculated by the PISA server41. Taken together, these findings suggest that the spatial arrangement, interaction mode, and oligomeric packing of apisimin are markedly different from the closest structural homolog. Therefore, the apisimin structure reveals an unusual interaction mode.
Extensive hydrophobic interactions are crucial for oligomer
In the asymmetric unit (MRJP12-apisimin2-Osl4), there exist two interfaces between MRJP1 protomers; symmetric intermolecular C-terminal and N-terminal interactions, and interactions between intermolecular antiparallel C-terminal β-sheets β31 (Fig. 4a–d). These interactions are strong, with a buried interface area of 771 Å2, and include extensive hydrophobic interactions, six salt bridges, and 16 hydrogen bonds (Fig. 4b–d). Most of the interacting residues are positioned at both termini, including Glu25, Lys29, Pro32, Arg342, Glu409, Leu410, Ile411, Asn413, Thr414, Arg415, Asp422, Thr424, Phe426, Ile428, Ser429, Ile430, and His431. Moreover, the temperature factors of the residues in the C-terminal β31 are 42% lower than the mean value, suggesting β31 is involved in forming a tight complex (Supplementary Fig. 6C). Additionally, two apisimin and the inner pair of Osl molecules also greatly contribute to the binding interface (Figs. 4e, 5b), resulting in a total buried interface of 1760 Å2 for each MRJP1, which strongly stabilises the dimer.
For apisimin and Osl molecules in the MRJP1 dimer, most of the interactions are also hydrophobic. Apisimin interacts with two MRJP1 and three Osl molecules with a total buried interface area of 1168 Å2 (Fig. 4e). For the inner two Osl molecules, each is totally enveloped by two MRJP1 molecules, one apisimin, and the other three Osl molecules (Fig. 5a–c and Supplementary Fig. 6F). For the outer two Osl molecules, each interacts with two apisimin molecules and the other three Osl molecules (Fig. 5d), with 92% of the total buried surface involved in the interaction. Meanwhile, no interactions occur between MRJP1 and the outer two Osl molecules. Interestingly, the inner and outer Osl molecules are almost parallel to the N-terminal or C-terminal α-helices of apisimin, respectively, ensuring maximal interaction. Taken together, these strong interactions render the entire MRJP1 dimer highly stable.
MRJP1 oligomer is mainly tetrameric and is pH-dependent
Careful structural analysis revealed two symmetric interfaces between two apisimin molecules from two neighbouring MRJP1 dimers. Each interface is formed mainly by hydrophobic interactions and four additional hydrogen bonds (Fig. 6a, b and Supplementary Fig. 6G). Interestingly, only the C-terminal α-helices of the four apisimin molecules are involved in forming the interfaces. The residues involved in interface formation are conserved across different species (Supplementary Fig. 8C), revealing the importance of apisimin in the MRJP1 oligomer. The total buried interface area reaches 1117 Å2, slightly less than the value of 1600 ± 400 Å2 that is generally believed to be of physiological significance42. Furthermore, the free energy of assembly/association (ΔGass) of the whole-MRJP1 oligomer was −9.3 kcal/mol, as calculated by the PISA server41. A large, negative value of ΔGass is indicative of a strong interface, suggesting the interface between the two apisimin molecules in the two MRJP1 dimers is sufficient for tetramer formation. Taken together, the evidence suggests the complete MRJP1 oligomer (MRJP14-apisimin4-Osl8) is formed by apisimin-dependent dimerisation of MRJP1 dimers, and the four apisimin molecules are the key bridges holding the complex together.
To further investigate the oligomeric states of MRJP1 oligomers in solution, we performed gel filtration, sedimentation velocity experiments, and small-angle X-ray scattering (SAXS) analyses. The molecular weight of the native MRJP1 oligomer was determined by analytical ultracentrifugation to be 254 kDa (Fig. 6c). MRJP1 monomer, dimer, and oligomer were observed in analytical ultracentrifugation. Gel filtration and SAXS analyses further confirmed that the MRJP1 oligomer mainly exists as a MRJP1 tetramer at near-neutral pH (Supplementary Figs. 10C, 9). Therefore, the solved structure was likely that of the predominant oligomeric state in solution, and not an artefact of crystal packing. Moreover, gel filtration chromatography showed that the MRJP1 tetramer existed universally in RJ collected from the nectar of different plants and honeybee species (Supplementary Fig. 10A, B).
Interestingly, the MRJP1 oligomer has been reported to adopt different assembly states and molecular weights, ranging from 231 to 450 kDa, and large soluble aggregates have also been reported1,2,10,17,28,32,33. Upon reviewing the literature, we noticed that these analyses were carried out under different conditions. We think that pH might be the main cause. To evaluate the influence of pH on the oligomeric state of MRJP1, we performed gel filtration experiments on the purified MRJP1 oligomer using a Superdex-200 HR10/300 column (GE Healthcare) at a variety of different pH values (Fig. 6d). Between pH 5 and pH 9, the peak of the MRJP1 oligomer eluted at 13.6 mL, corresponding to the molecular weight of the MRJP14-apisimin4-Osl8 complex. However, at pH 4.5 and pH 4, the peak of higher oligomer eluted at 9.9 mL, close to the void volume of the column, indicating the presence of larger soluble aggregates. To eliminate the possibility that MRJP1 might behave differently depending on treatment, we purified MRJP1 from RJ in buffer at a different pH, and the results were comparable to the original pH (Supplementary Fig. 10C). By contrast, the behaviour of the MRJP1 monomer did not change at different pH. Taken together, these results suggest large soluble aggregates and the MRJP14-apisimin4-Osl8 complex are interchangeable, consistent with previous observations3,43.
The honeybee is one of the most familiar and important insects for humans and plants, critical for apiculture and pollination, and its benefits are arguably immeasurable. RJ are natural products that are vital for honeybee development and reproduction, and widely used in human health-promoting foods, and cosmetics. The MRJP1 monomer (royalactin) rather than the oligomer is reported to be the key factor inducing queen differentiation in honeybees10,12. This raises the question why the more abundant MRJP1 oligomer does not have this function. Our structural analyses of the MRJP1 oligomer show that 10% surface of MRJP1 is involved in binding apisimin and Osl, especially in the C-terminal region. We suspect that the binding of apisimin and Osl in the MRJP1 oligomer block driving queen development through the downstream signalling pathways.
Over the years, the honeybee MRJP1 oligomer has been reported to be a hexamer, pentamer28, or tetramer32, with varying molecular mass43. Moreover, it is unclear which factors influence the oligomerisation of MRJP1, and whether the oligomeric state varies with the source of RJ. In this work, the native MRJP1 oligomer was purified from RJ and determined by analytical ultracentrifugation as a 254 kDa assembly at neutral pH. The structure of the MRJP1 oligomer was determined at the same pH as the insect midgut, and therefore likely represents the physiologically relevant and native structure. MRJP1 oligomer mainly exists as a MRJP14-apisimin4-Osl8 complex and adopts an elegant H-like shape at near-neutral pH, and this was further confirmed by SAXS analysis (Fig. 6c and Supplementary Fig. 9). Interestingly, although the MRJP1 tetramer is the most common state, monomers and dimers were also observed. To investigate why, we performed a series of structural analyses. Apisimin functions as a dimer joining the protein complex, stabilising the complete MRJP14-apisimin4-Osl8 tetramer. The tetramer interface area of 1117 Å2 is lower than the total dimeric interface area of 1760 Å2, and slightly less than the lower limit of 1600 ± 400 Å2 that is the assumed cutoff for physiologically relevant interfaces. Therefore, the MRJP1 tetramer is not highly stable, and may disassociate into dimers or even monomers to some extent, which could contribute to its in vivo function in honeybees.
We detected two types of MRJP1 oligomer in solution, and self-assembly was pH-dependent and reversible, as confirmed by size exclusion chromatography and MS. The MRJP1 tetramer was completely converted to large aggregates when the pH was lower than 4.5, close to the pH of natural RJ. Moreover, this phenomenon is universally conserved among all different sources of MRJP1, including various nectar plants and honeybee species (Supplementary Fig. 10A, B). Further structural analyses suggest that nine histidine residues (with pK2 = 6.0) on the protein surface may play crucial roles in the pH-dependent self-association of the MRJP1 oligomer. Our results of pH-dependent self-assembly size are similar to the recent online publication11. The larger soluble aggregates of MRJP1 oligomer form long fibrous structures that build the basis for the high viscosity of RJ to hold queen larvae on the RJ surface and prevent them falling out of their cells.
Structural analyses revealed that both termini of MRJP1 play key roles in forming the MRJP1 dimer (Fig. 4a−d). Indeed, the C-terminus of MRJP1 interacts with the other MRJP1 of the dimer, as well as with apisimin and Osl, thereby determining the formation of the MRJP1 dimer. Sequence alignment revealed that both termini and the regions involved in forming the MRJP1 dimer are highly diverse across the MRJP family (Supplementary Fig. 8B), which may explain why oligomers were not found for other MRJPs, with the exception of MRJP3, which forms a trimer44.
The surface of the MRJP1 oligomer is highly negatively charged at near-neutral pH (Figs. 5a, 6a), presumably resulting in repulsion between oligomers, making them resistant to aggregation under near-neutral conditions, and thus highly stable. Moreover, extensive interactions are involved in forming the MRJP1 oligomer, and many surfaces are protected from proteases by other MRJP1, apisimin, and Osl molecules. Thus, the MRJP1 oligomer appears to be heat-stable and more resistant to degradation than monomer during storage30,45. Indeed, our synchrotron radiation circular dichroism experiments confirmed that the denaturation temperature of the MRJP1 oligomer was much higher than that of the monomer (Tm = 55 °C vs. 43 °C, Supplementary Fig. 11), consistent with previously reported observations30,45. Therefore, the MRJP1 oligomer is more stable than the MRJP1 monomer. Since other MRJPs appear not to form oligomers, their stability may also be much lower than that of the MRJP1 oligomer, which may explain why the MRJP1 oligomer is the major storage protein in RJ.
The honeybee provides a convenient classical model for understanding the biology of insects, epigenetics, and social systems. Diploid eggs become queens or workers depending on whether larvae are fed royal jelly (RJ) or not4,46. The role of RJ for caste determination in the honeybee is the textbook classic paradigm for environmental caste differentiation in social insects. The association between RJ and caste formation has been known for >100 years, but the identity of the component(s) in RJ that induce queen development has remained elusive and controversial3,10,12. It is believed that MRJP1 play pivotal roles in honeybee development and reproduction11. Our current findings suggest that large soluble aggregates of the MRJP1 oligomer in RJ might provide a means for storing the physiologically active MRJP1 in the colony. The pH of the insect midgut is normally above 5.5. After ingestion by bee larvae, the large soluble aggregates of the MRJP1 oligomer would be converted to the tetramer or even the monomer in the midgut. Interestingly, proteomic analyses revealed that queen development was not triggered by intact MRJP1 oligomer complexes, but rather by proteolytic products generated in the digestive tract47. Moreover, because the amino acid sequence of apisimin has several protease cleavage sites, including those recognised by chymotrypsin and trypsin, it also provides an opportunity to release MRJP1 from the MRJP1 oligomer in the honeybee digestive system. Moreover, apisimin can be separated from the MRJP1 oligomer under mild conditions28, implying easy conversion between oligomeric and monomeric MRJP1. Thus, the MRJP1 oligomer might be digested at somewhat lower rate than the monomer in the digestive tract, thereby fulfilling some biological functions.
Sand fly LJM11 confers protective immunity against Leishmania infection due to its high affinity for serotonin38. Since both MRJP1 and LJM11 belong to the same Yellow protein family, we investigated whether the MRJP1 oligomer and/or monomer could bind serotonin. Isothermal titration calorimetry (ITC) results showed that neither form could bind serotonin, at least not under the experimental conditions employed (Supplementary Fig. 12A, B). Structural alignment of the MRJP1 and Ljm11 proteins revealed that the central hole in MRJP1 is too small, and residues near the hole in MRJP1 would sterically clash with serotonin (Supplementary Fig. 12C−E). Moreover, we failed to find LJM11 when searching for homologs of the MRJP1 structure using the DALI server in protein data bank, revealing the low structural similarity between MRJP1 and LJM11. Therefore, unlike LJM11, MRJP1 cannot bind serotonin, implying a different function for MRJP1.
MRJP1 is the most abundant glycoprotein among the soluble proteins in RJ, and has three predicted glycosylation sites at Asn28, Asn144, and Asn17748. Our experiments clearly showed that N-linked glycan sites hindered the formation of tight crystal contacts. Interestingly, electron density for N-acetylglucosamine (NAG) was observed at glycosylation site Asn144 in the crystal structure (Supplementary Fig. 3). Although PNGase F was effective for deglycosylating N-linked glycans, NAG interacts with surrounding residues including Asn84, Val143, and Gln147, hindering its removal by PNGase F under mild conditions. Observation and structural analysis confirmed partial deglycosylation of the MRJP1 oligomer under native conditions (Supplementary Fig. 3A). Our structure therefore directly confirmed that native MRJP1 is glycosylated. Hypertension is a major risk factor that leads to cerebral stroke, heart failure, and acute myocardial infarction in humans. MRJP1 is a potential candidate for anti-hypertension, but interestingly, only glycosylated MRJP1 significantly inhibits the migration of human vascular smooth muscle cells (VSMCs), and therefore possesses anti-hypertension activity, unlike its deglycosylated form24, revealing the importance of glycosylation.
Osl is a natural product first identified in marine organisms, which is also found in RJ and honeybees35,36. In the present study, electron density, EI-GC/MS, and 1H and 13C NMR results confirmed its presence in the MRJP1 oligomer (Fig. 1c and Supplementary Fig. 7). To our knowledge, Osl has not been reported to exist in a protein complex. Although unexpected, Osl is a tightly bound cofactor in the MRJP1 oligomer. Rearing experiments reveal that Osl might greatly promote the growth of honeybee larvae. Importantly, Osl is known to significantly decrease cholesterol levels in both serum and liver49, and the MRJP1 oligomer exhibits high hypocholesterolemic activity in rats, although the mechanism remains to be elucidated22. Our discovery of Osl in the MRJP1 oligomer may explain its hypocholesterolemic effect, and provides a potential new strategy for lowering cholesterol in humans. Moreover, Osl is reported to have anti-aging effects and neuroprotective functions50, suggesting the MRJP1 oligomer may be used to develop promising neuroprotective drugs. Osl, constituting 49–58% of RJ sterols, also has a high-level content in the queens, but very little in worker honeybees or pollens51. Osl is an essential sterol in bee metabolism influencing moulting and the development of ovaries52. Moreover, it shows the strongest estrogenic activity among the main small compounds in RJ35. Therefore MRJP1 might play important roles in queen development, and have the potential to improve menopausal symptoms and bone formation, and prevent osteoporosis in humans.
We believe our findings will contribute to a better understanding of the unique pharmacological effects of MRJP1 in humans, and they provide insights into the mechanisms underlying its physiological functions in bees, including caste differentiation, learning ability, colony organisation, and development. Importantly, MRJPs have great potential as drug candidates for promoting human health, due to their hypocholesterolemic, anti-hypertension, antibiotic, anti-tumour, and cell growth-promoting activities, and their ability to prolong longevity and protect the immune system. For example, the MRJP1 glycoprotein harbours three antimicrobial jelleine peptides53.
Fresh royal jelly (RJ) samples were collected from Apis mellifera and Apis cerana colonies located in several regions of Zhejiang province during the flowering periods of different nectar plants. All RJ samples were stored at −20 °C until analyses.
One gram of RJ was dissolved in 10 mL of 40 mM phosphate buffer (pH 8.0). The suspension was centrifuged (25,000 g) for 15 min at 4 °C to separate the supernatant and pellet, and the supernatant was filtered with the 0.45 μm membrane filter. After adding 30 mL 20 mM Tris-HCl (pH 8.0), the filtered fluid was concentrated to 5 mL by ultrafiltration through a 30 kDa MW cutoff membrane (Millipore). The concentrated solution was further purified by the Q sepharose (Bio-rad). The protein was eluted with a linear gradient of NaCl from 0 to 1.0 M at a flow rate of 0.5 mL/min, and the elution was detected by SDS-PAGE and Coomassie blue staining. The fractions containing MRJP1 were collected and concentrated to 2 mL. MRJP1 collected from Q sepharose was applied to a Hiload Superdex-200 26/600 column (GE Healthcare) previously equilibrated with the buffer containing 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl. The column was then eluted at a flow rate of 1.5 mL/min, and the fractions containing MRJP1 monomer and oligomer protein were collected, respectively.
N-linked deglycosylation was conducted with PNGase F (peptide:N-glycosidase F). PNGase F was added to MRJP1 protein and incubated overnight at 4 °C. Then the mixture was changed to buffer containing 20 mM Tris-HCl (pH 8.0) and 20 mM NaCl, then purified using UNOTM Q (Bio-rad). Fractions containing MRJP1 were collected and concentrated to 15 mg/mL for crystallization.
MALDI-TOF-MS of MRJP1 oligomer
The time-of-flight instrument (Autoflex III TOF/TOF, Bruker Daltonics) fitted with a 337-nm nitrogen laser in linear mode was used for peptide molecular weight determination. Peptides were analyzed in reflector mode with delayed extraction using an acceleration voltage of 20 kV. Data were generally collected after ionization at 100 MHz in the m/z 500 to 4000 range with the instrument calibrated externally using a mixture of human angiotensin II, ACTH clip (residues 18–39) and bovine insulin.
The purified MRJP1 oligomer was desalted by ultrafiltration using a 30 kDa MW cutoff membrane Amicon Ultra-15 centrifugal filter units (Millipore) to 2 mg/mL. Then the protein was digested by trypsin overnight. A total of 1 μL of the digested peptide was spotted and dried naturally and 2 μL of 5 mg/mL MALDI matrix α-cyano-4-hydroxycinnamic acid in 50% acetonitrile/0.1% TFA was spotted on the sample spot. The solvent evaporated and the peptide/matrix co-crystallized.
Liquid chromatography/electrospray mass spectrometry
The HPLC Series 1100 system used for the analysis of the digested peptides was composed of a binary pump, a column temperature stabilizer and a diode array detector (Agilent, Waldbronn, Germany). Agilent ChemStation software was used for instrument control and data processing. A reversed-phase HPLC column (Zorbax 300SB-C18 150 × 2.1 mm, 5 μm) was used for peptide separation with the mobile phase flow rate set at 0.2 mL/min (solution A: 0.1% TFA in H2O (V/V); solution B: 0.1% TFA in acetonitrile (V/V)). For the separation, the solutions A and B were used as the gradient elution. The gradient program was set as follows: 5% B (0 min), 5% B (5 min), 40% B (40 min), 75% B (70 min), 75% B (80 min), 5% B (85 min) for total runtime of 85 min.
The mass spectra were obtained using a Finnigan LCQ ion trap mass spectrometer (Thermo electron, San Jose, CA, USA) equipped with an electrospray ionization source (Spectronex AG, Basel, Switzerland). The spray voltage and heated capillary were 4.5 kV and 275 °C, respectively. The scan range was set from m/z 400 to 2000. After electrospray ionization, tandem MS (MS/MS) data were obtained by collisional activating of the solute molecules colliding with argon gas. The collision energy value was set at 35%.
Expression and purification of recombinant MRJP1
The cDNA encoding the mature protein, excluding the signal peptide, of full-length MRJP1 (58–1296 bases, GenbankTM accession number D79207) and various fragments were subcloned into pET19b (Merck), pGEX-4T-2 (GE), pCold™ TF (TaKaRa) or some other vectors using the primers in Supplementary Table 1. The method was similar to the reference10. Briefly, Escherichia coli strain BL21 (DE3) pLysS cells (Merck) harboring the plasmids were grown at 37 °C to an OD600 of ~0.8 in LB medium containing ampicillin (50 μg/mL). Then isopropyl β-D-thiogalactoside (0.5 mM) was added to induce protein expression, and culture was continued at 37 °C for 3 h or at 18 °C for 17 h. Then, the cells were collected by centrifugation, suspended in buffer A (50 mM Tris-HCl pH 8.0 containing 500 mM NaCl), and disrupted with a Tomy Ultrasonic Disruptor at 20 kHz and 80 W for 80 s. The sonicated solution was centrifuged at 20,000×g for 30 min at 4 °C, and the supernatant was applied to a His-Bind or GST resin affinity column and eluted with 400 mM imidazole or 10 mM glutathione, respectively. The Tag was removed by the corresponding protease at a 1:10 (w/w, protease/protein) ratio for 6 h at 4 °C to remove His, GST or TF Tag. The fractions containing MRJP1 were collected and applied to a HiPrepTM 16/60 Sephacryl S-200 column. The column was eluted with Buffer A at a flow rate of 1.0 mL/min, and the fractions containing MRJP1 were pooled, dialyzed against distilled water and lyophilized. All the protein purification procedures were performed at 4 °C.
For the recombinant expression of MRJP1 in yeast, the method was according to the publication54.
The method used is slightly modified from the publication34. The buffer was changed to 50 mM HEPES (pH 7.5), 250 mM NaCl at protein concentrations of 1 mg/mL. A total of 20 μl freshly prepared 1 M dimethylamine-borane complex (ABC) and 40 μl 1 M formaldehyde were added per mL protein solution, and the reactions were gently incubated at 4 °C for 2 h. A further 20 μl ABC and 40 μl formaldehyde per mL protein solution were added and the incubation continued for 2 h. Then a final addition of 10 μl ABC and 20 μl formaldehyde per mL protein solution, the reaction was incubated overnight at 4 °C. The protein was further purified by size exclusion chromatography columns in buffer A.
Analytical size exclusion chromatography
The MRJP1 proteins were applied to a Superdex-200 10/300 column (GE Healthcare) equilibrated with a buffer containing 150 mM NaCl at 20 mM Tris (pH 7.0, 8.0, 9.0) or 20 mM sodium citrate (pH 4.0, 4.5, 5.0, 6.0). To compare the different states between MRJP1 oligomer and monomer, protein was exchanged to the corresponding buffer before loaded onto the Superdex-200 column. The proteins were detected by SDS-PAGE followed by Coomassie blue staining.
Extensive crystallization screens were performed at both 18 °C and 4 °C for native, lysine methylated and deglycosylated MRJP1 with concentration at 20–25 mg/ml. Monomer MRJP1 failed to grow a crystal after many trials. For native MRJP1 oligomer crystal screen, only needle clusters were obtained in several crystal conditions and the extensive optimization could not change the crystal shape. Lysine methylated MRJP1 oligomer were prone to crystallization in 15% PEG8000 with a pH range of 6.5–8.5. Large cube crystals were obtained after optimization. However, they diffracted poorly, usually at 8–10 Å resolution. Moreover, a skin was formed in the crystallization drop, which made it difficult to harvest the crystals. The breakthrough was made with deglycosylated proteins, three different forms of crystals were obtained. The cylinder crystals diffracted poorly, with the best diffraction to 6.8 Å. The optimization could not decrease the nucleation of crystals, and a skin was also found on the crystallization drop. Rodlike crystals were so fragile that they disappeared when harvesting. Cuboid crystals obtained using seeding method appeared in 2 days and grew to full size in 2 weeks using the hanging-drop vapor-diffusion method. The well buffer contained 0.1 M Tris, pH 8.5 and 2.0 M ammonium phosphate.
Diffraction data collection and structure determination
All data sets were collected on beamlines BL17U and BL19U at the Shanghai Synchrotron Radiation Facility (SSRF) or BL5A at the Photon Factory (KEK). Data were indexed, integrated and scaled using HKL200055, and processed according to CC1/256.
For no homologous protein of MRJP1 was found in the Protein Data Bank (PDB), we tried to solve the MRJP1 oligomer structure by multiple isomorphous replacement (MIR) or multiple-wavelength anomalous diffraction (MAD) method. More than 100 MRJP1 oligomer crystals, soaked in the reservoir solution mixed with different heavy metals or haloids such as Pt, Au, Hg, Pb, Br, or I under variable concentration and time, were screened. Some crystals after heavy metal soaking still displayed poor or even no diffraction, and the others had good diffraction but with weak anomalous signal of heavy metal. We also tried the co-crystallization of MRJP1 and different concentration of several kinds of heavy metals or haloids. Nevertheless these crystals with strong diffraction had no specific heavy metal ions or haloids bound. Moreover, we also expressed the selenomethionyl MRJP1 in both E. coli and yeasts, but we failed in the generation of diffraction-quality crystals.
After the failure of extensive trials to solve the phase by MIR or MAD methods, we searched for the weak homology of MRJP1 and found a structure of a Yellow family protein LJM11 from the saliva of the sand fly lutzomyia longipalpiss38. The sequence homology was low between LJM11 and MRJP1 with a value of 16.9%. Using the monomer structure of LJM11 (PDB: 3Q6K) as the search model, an initial molecular replacement (MR) solution of MRJP1 was found by BALBES57 after extensive trials of different resolution and space groups (H32 or H3). The best solution found two monomers with an MR score of 2.20 and an Rwork/Rfree value of 0.538/0.568 for space group H32. After removing the inappropriate main and side chains in the model using the program COOT58, the optimized monomer structure was used to find the right position by program BALBES again. The resulting model was then fed back to the program IPCAS59 to combine single isomorphous replacement phasing and partial structure information to break the phase ambiguity intrinsic in the MR method. The primitive density map has a relatively clear outline, with many recognizable alpha-helix bundles.
Then we iteratively refined and built the model using CNS60, IPCAS59, the CCP4 suite61 and the autobuild program in the Phenix62. According to the constraints of non-crystallographic symmetry with twofold, the non-rational main and side chains were adjusted on the electron density map using the program COOT. After more than 100 cycles of manual rebuilding and refining, the best solution stopped at an Rwork/Rfree value of 0.32/0.36. At this point, the structures of two MRJP1 molecules were well fitted into electron-density maps with clear α-helices and β-strand bundles. Interestingly, two anti-parallel α-helices and four blobs density were found and fit to two apisimin and four 24-methylenecholesterol molecules, respectively. After more than 40 rounds of position modulation and five rounds of simulated annealing correction, we continued to modulate the non-rational main and side chains to make R factor, Φ–Ψ angle and B factor lying in the allowed regions. Finally, the crystal structure of MRJP1 oligomers was improved by non-crystallographic symmetry restraints. The final structure was refined to a resolution of 2.65 Å in the space group H32, with an Rwork of 23.9% and an Rfree of 27.5% (Table 1). The whole-MRJP1-Apisimin-24-methylenecholesterol ternary structure was obtained by a twofold axis using a symmetric operation.
All structural figures in this article were drawn by using the PyMOL program63. The Adaptive Poisson-Boltzmann Solver64 combined with PDB2PQR was used for molecular electrostatics calculations.
Sedimentation velocity (SV) experiments were performed in a Beckman/Coulter XL-I analytical ultracentrifuge using double-sector or six-channel centerpieces and sapphirine windows. Before SV experiments, an additional protein purification step was applied using size exclusion chromatography in buffer B containing 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl. The experiments were conducted at 42,000 rpm and 4 °C using interference detection and double-sector cells loaded at approximate 0.5 mg/mL for MRJP1 proteins. The SV data were analyzed using the SEDFIT and SEDPHAT programs65.
Small angle X-ray scattering experiments
Small angle X-ray scattering (SAXS) data (Supplementary Table 2 and Supplementary Data 1) were collected at the BioSAXS station (1W2A) of the BSRF or beamline BL19U2 of the SSRF, using previously published methods66. Briefly, the proteins were subjected to size exclusion chromatography with a buffer containing 20 mM sodium phosphate (pH 8) and 150 mM NaCl. The protein concentrations were in a gradient concentration of 0.1, 0.2, 0.5, 1, 2, 5, 10 mg/mL, and the data of the protein samples were collected at 1.54 Å with a distance of 1.64 m from the detector. Data collection time of 5 min was used for all samples split into two equal time frames to assess and remove effects from radiation damage to the samples. Individual data were processed by FIT2D67. The average of the scattering from the buffer alone before and after each sample was used for background subtraction. The theoretical scattering curves were fitted to the experimental scattering curve using MES.
Synchrotron radiation circular dichroism spectra
The Synchrotron radiation circular dichroism (CD) spectra of MRJP1 proteins were collected on beamline 4B8 of the Beijing Synchrotron Radiation Facility. All proteins were changed to a buffer solution 20 mM sodium phosphate (pH 8.0), filtered and accurately weighed. All spectra were recorded three times using a 0.007 mm optical path length quarts SUPRASIL cell, over a wavelength range of 175–255 nm in 1 nm steps and a dwell time of 1 s per wavelength point. A pure solvent baseline was measured with the same cell and subtracted. CD spectra were also measured at 2 °C or 5 °C intervals from 5 °C to 85 °C. The sample was equilibrated 5 min at each temperature prior to the measurement.
All spectra were processed by the CDtool software package. The machine unit (mdeg) was converted into the per residue molar absorption unit, delta epsilon (Δε) in M cm-1, by normalization with respect to protein concentration and path length. Secondary structure content analysis of the spectra was based on the CDSSTR reference set 6 from the software package CDPro68.
Isothermal titration calorimetry
Experiments were performed on a Nano ITC instrument (TA Instruments) at 20 °C. An additional protein purification step was applied using size exclusion chromatography in buffer B before applying to ITC. MRJP1 oligomer was diluted to 2 mg/mL using buffer B. A total of 200 mM serotonin was dissolved in buffer B and adjusted to pH 8.0 with NaOH. A total of 2 mM serotonin was obtained by diluting 200 mM stock solution with buffer B. A total of 2 mM serotonin in the syringe was titrated against 2 mg/mL MRJP1 proteins in the cell. ITC data were processed with NanoAnalyze software (TA Instruments).
Isolation of small molecules from MRJP1 oligomer
A total of 5 mL 2 mol/L NaOH was added to 1 g purified MRJP1 oligomer, then the solution was extracted with 30 mL n-hexane for three times. The combined n-hexane part was washed by water, then was evaporated to remove the organic solvents.
GC–MS of 24-methylenecholesterol
The small molecule isolated from the purified MRJP1 oligomer was confirmed by EI-GC–MS analysis via a QP-2010 mass spectrometer (Tokyo, Japan). The GC was equipped with a DB-5MS (25 m × 0.25 mm × 0.25 µm) capillary column (Agilent, Santa Clara, CA, USA). The temperature of the injector was kept at 300 °C, and 1.0 µL of the sample solution was injected in high pressure and splitless mode. The flow control mode was a constant linear velocity (36.8 cm/s). Helium (purity ≥99.999%) at 1.0 mL/min was used as the carrier gas. The oven temperature was programmed as follows: the initial temperature was 100 °C (maintained for 1 min), 10 °C/min ramp, 150 °C, 30 °C/min, 290 °C for 15 min. The running time for each assay was ~26 min.
The conditions were set as follows: mass spectra and retention times were obtained in EI mode (70 eV), with an ion source temperature of 200 °C, and a transfer line temperature of 280 °C. MS data were acquired in the scan mode (m/z 40–1000) for quantization with 4 min of solvent delay. The GC–MS Solution Version 2.40 and NIST database were used for data processing and compound searching, respectively. The data are consistent with the standard spectra in the website (http://www.massbank.jp/jsp/FwdRecord.jsp?type=disp&id=JP008184) with molecular formula C28H46O (MS m/z: 398).
NMR spectroscopic data for 24-methylenecholesterol
The small molecule isolated from the purified MRJP1 oligomer was further confirmed by NMR analysis. NMR spectra in CDCl3 were recorded on a Bruker AV III HD-600 instrument for 1H and 13C using standard pulse programs and acquisition parameters. Chemical shifts were reported in δ (ppm) referencing to the NMR solvent used. The data were consistent with the references37 and69.
1H NMR (600 MHz, CDCl3) δ: 5.36 (d, J = 4.8 Hz, 1H, 6-H), 4.71 (s, 1H, 28-H), 4.66 (d, J = 1.2 Hz, lH, 28-H), 3.55–3.50 (m, lH, 3α-H), 2.31–2.28 (m, 1H), 2.25–2.21 (m, 2H), 2.12–2.07 (m, 1H), 2.03–1.85 (m,2H), 1.61–1.52 (m, 4H), 1.51–1.49 (m, 2H), 1.45–1.40 (m, 3H), 1.31–1.25 (m, 2H), 1.25 (s, 2H), 1.19–1.12 (m, 3H), 1.11–1.05 (m, 2H), 1.03 (d, J = 6.6 Hz, 3H, 26-H3), 1.02 (d, J = 7.2 Hz, 3H, 27-H3), 1.01 (s, 3H, 19-H3), 0.99–0.97 (m, 1H), 0.95 (d, J = 6.6 Hz, 3H, 21-H3), 0.93–0.87 (m, 1H), 0.68 (s, 3H, 18-H3).
13C NMR (150 MHz, CDCl3) δ: 156.9 (24-C), 140.8 (5-C), 121.7 (6-C), 106.0 (28-C), 71.8 (3-C), 56.8 (14-C), 56.1 (17-C), 50.2 (9-C), 42.4 (4-C), 42.4 (13-C), 39.8 (12-C), 37.3 (1-C), 36.5 (10-C), 35.8 (20-C), 34.8 (23-C), 33, 9 (25-C), 32.0 (7-C), 31.9 (8-C), 31.7 (2-C), 31.0 (22-C), 28.2 (16-C), 24.3 (15-C), 22.0 (27-C), 21.9 (26-C), 21.1 (11-C), 19.4 (19-C), 18.7 (21-C), 11.9 (18-C).
The accession number for the coordinates of the MRJP1-apisimin-24-methylenecholesterol complex reported in this paper is PDB: 5YYL. 24-methylenecholesterol has been added to the PDB component dictionary with code 94R. Further data are available from the corresponding author upon reasonable request.
Simuth, J. Some properties of the main protein of honeybee (Apis mellifera) royal jelly. Apidologie 32, 69–80 (2001).
Tamura, S., Kono, T., Harada, C., Yamaguchi, K. & Moriyama, T. Estimation and characterisation of major royal jelly proteins obtained from the honeybee Apis merifera. Food Chem. 114, 1491–1497 (2009).
Buttstedt, A., Ihling, C. H., Pietzsch, M. & Moritz, R. F. Royalactin is not a royal making of a queen. Nature 537, E10–E12 (2016).
Kucharski, R., Maleszka, J., Foret, S. & Maleszka, R. Nutritional control of reproductive status in honeybees via DNA methylation. Science 319, 1827–1830 (2008).
Buttstedt, A., Moritz, R. F. & Erler, S. Origin and function of the major royal jelly proteins of the honeybee (Apis mellifera) as members of the yellow gene family. Biol. Rev. Camb. Philos. Soc. 89, 255–269 (2014).
Nozaki, R. et al. A rapid method to isolate soluble royal jelly proteins. Food Chem. 134, 2332–2337 (2012).
Schmitzova, J. et al. A family of major royal jelly proteins of the honeybee Apis mellifera L. Cell Mol. Life Sci. 54, 1020–1030 (1998).
Drapeau, M. D., Albert, S., Kucharski, R., Prusko, C. & Maleszka, R. Evolution of the Yellow/Major Royal Jelly Protein family and the emergence of social behavior in honey bees. Genome Res. 16, 1385–1394 (2006).
Hojo, M., Kagami, T., Sasaki, T., Nakamura, J. & Sasaki, M. Reduced expression of major royal jelly protein 1gene in the mushroom bodies of worker honeybees with reduced learning ability. Apidologie 41, 194–202 (2010).
Kamakura, M. Royalactin induces queen differentiation in honeybees. Nature 473, 478–483 (2011).
Buttstedt, A. et al. How honeybees defy gravity with royal jelly to Raise Queens. Curr. Biol. (2018).
Kamakura, M. Kamakura replies. Nature 537, E13 (2016).
Kucharski, R., Maleszka, R., Hayward, D. C. & Ball, E. E. A royal jelly protein is expressed in a subset of Kenyon cells in the mushroom bodies of the honey bee brain. Naturwissenschaften 85, 343–346 (1998).
Majtan, J., Kumar, P., Majtan, T., Walls, A. F. & Klaudiny, J. Effect of honey and its major royal jelly protein 1 on cytokine and MMP-9 mRNA transcripts in human keratinocytes. Exp. Dermatol. 19, e73–e79 (2010).
Watanabe, K. et al. Stimulation of cell growth in the U-937 human myeloid cell line by honey royal jelly protein. Cytotechnology 26, 23–27 (1998).
Watanabe, K. et al. Growth stimulation with honey royal jelly DIII protein of human lymphocytic cell lines in a serum-free medium. Biotechnol. Tech. 10, 959–962 (1996).
Kimura, M. et al. 350-kDa royal jelly glycoprotein (Apisin), which stimulates proliferation of human monocytes, bears the β1-3Galactosylated N-Glycan- Analysis of the N-Glycosylation Site. Biosci. Biotech. Biochem. 67, 2055–2058 (2003).
Kamakura, M. & Sakaki, T. A hypopharyngeal gland protein of the worker honeybee Apis mellifera L. enhances proliferation of primary-cultured rat hepatocytes and suppresses apoptosis in the absence of serum. Protein Expr. Purif. 45, 307–314 (2006).
Kamakura, M., Suenobu, N. & Fukushima, M. Fifty-seven-kDa protein in royal jelly enhances proliferation of primary cultured rat hepatocytes and increases albumin production in the absence of serum. Biochem. Biophys. Res. Commun. 282, 865–874 (2001).
Brudzynski, K., Sjaarda, C. & Lannigan, R. MRJP1-containing glycoproteins isolated from honey, a novel antibacterial drug candidate with broad spectrum activity against multi-drug resistant clinical isolates. Front. Microbiol. 6, 711 (2015).
Vezeteu, T. V., Bobis, O., Moritz, R. F. & Buttstedt, A. Food to some, poison to others—honeybee royal jelly and its growth inhibiting effect on European Foulbrood bacteria. Microbiologyopen 6, e00397 (2017).
Kashima, Y. et al. Identification of a novel hypocholesterolemic protein, major royal jelly protein 1, derived from royal jelly. PLoS ONE 9, e105073 (2014).
Nakaya, M. et al. Effect of royal jelly on bisphenol A-induced proliferation of human breast cancer cells. Biosci. Biotechnol. Biochem. 71, 253–255 (2007).
Feng, M. et al. In-depth N-glycosylation reveals species-specific modifications and functions of the royal jelly protein from western (Apis mellifera) and eastern honeybees (Apis cerana). J. Proteome Res. 14, 5327–5340 (2015).
Fan, P. et al. Functional and proteomic investigations reveal major royal jelly protein 1 associated with anti-hypertension activity in mouse vascular smooth muscle cells. Sci. Rep. 6, 30230 (2016).
Majtan, J., Kovacova, E., Bilikova, K. & Simuth, J. The immunostimulatory effect of the recombinant apalbumin 1-major honeybee royal jelly protein-on TNFalpha release. Int. Immunopharmacol. 6, 269–278 (2006).
Simuth, J., Bilikova, K., Kovacova, E., Kuzmova, Z. & Schroder, W. Immunochemical approach to detection of adulteration in honey: physiologically active royal jelly protein stimulating TNF-alpha release is a regular component of honey. J. Agric. Food Chem. 52, 2154–2158 (2004).
Tamura, S. et al. Molecular characteristics and physiological functions of major royal jelly protein 1 oligomer. Proteomics 9, 5534–5543 (2009).
Furusawa, T., Arai, Y., Kato, K. & Ichihara, K. Quantitative analysis of Apisin, a major protein unique to royal jelly. Evid. Based Complement. Altern. Med. 2016, 5040528 (2016).
Moriyama, T., Ito, A., Omote, S., Miura, Y. & Tsumoto, H. Heat resistant characteristics of major royal jelly protein 1 (MRJP1) oligomer. PLoS ONE 10, e0119169 (2015).
Bilikova, K. et al. Apisimin, a new serine-valine-rich peptide from honeybee (Apis mellifera L.) royal jelly: purification and molecular characterization. FEBS Lett. 528, 125–129 (2002).
Mandacaru, S. C. et al. Characterizing the structure and oligomerization of major royal jelly protein 1 (MRJP1) by mass spectrometry and complementary biophysical tools. Biochem 56, 1645–1655 (2017).
Kimura, Y., Washino, N. & Yonekura, M. N-linked sugar chains of 350-kDa royal jelly glycoprotein. Biosci. Biotechnol. Biochem. 59, 507–509 (1995).
Walter, T. S. et al. Lysine methylation as a routine rescue strategy for protein crystallization. Structure 14, 1617–1622 (2006).
Suzuki, K. M. et al. Estrogenic activities of Fatty acids and a sterol isolated from royal jelly. Evid. Based Complement. Altern. Med. 5, 295–302 (2008).
Barbier, M., Reichstein, T., Schindler, O. & Lederer, E. Isolation of 24-methylene-cholesterol from honey bees (Apis mellifica L). Nature 184(Suppl 10), 732–733 (1959).
Lu, W., Zhang, C., Zeng, L. & Su, J. Synthesis of polyhydroxysterols (V): efficient and stereospecific synthesis of 24-methylene-cholest-5-ene-3beta,7alpha-diol and its C-7 epimer. Steroids 69, 803–808 (2004).
Xu, X. et al. Structure and function of a “yellow” protein from saliva of the sand fly Lutzomyia longipalpis that confers protective immunity against Leishmania major infection. J. Biol. Chem. 286, 32383–32393 (2011).
Holm, L. & Rosenström, P. Dali server: conservation mapping in 3D. Nucleic Acids Res. 38, W545–W549 (2010).
Nakamura, A., Takumi, K. & Miki, K. Crystal structure of a thermophilic GrpE protein: insight into thermosensing function for the DnaK chaperone system. J. Mol. Biol. 396, 1000–1011 (2010).
Krissinel, E. & Henrick, K. Inference of macromolecular assemblies from crystalline state. J. Mol. Biol. 372, 774–797 (2007).
Lo Conte, L., Chothia, C. & Janin, J. The atomic structure of protein-protein recognition sites. J. Mol. Biol. 285, 2177–2198 (1999).
Cruz, G. C. N. et al. Calcium effect and pH-dependence on self-association and structural stability of the Apis mellifera major royal jelly protein 1. Apidologie 42, 252–269 (2011).
Okamoto, I. et al. Major royal jelly protein 3 modulates immune responses in vitro and in vivo. Life. Sci. 73, 2029–2045 (2003).
Kamakura, M., Fukuda, T., Fukushima, M. & Yonekura, M. Storage-dependent degradation of 57-kDa protein in royal jelly: a possible marker for freshness. Biosci. Biotechnol. Biochem. 65, 277–284 (2001).
Herb, B. R. et al. Reversible switching between epigenetic states in honeybee behavioral subcastes. Nat. Neurosci. 15, 1371–1373 (2012).
Chan, Q. W. et al. Honey bee protein atlas at organ-level resolution. Genome Res. 23, 1951–1960 (2013).
Kimura, Y., Nagai, H., Miyamoto, M., Kimura, M. & Yonekura, M. Identification of a royal jelly glycoprotein that carries unique complex-type N-glycans harboring the T-antigen (Galbeta1-3GalNAc) unit. Biosci. Biotechnol. Biochem. 74, 2148–2150 (2010).
Teshima, S., Kanazawa, A., Yoshioka, M. & Kitahara, K. Hypocholesterolemic effect of 24-methylenecholesterol and 7-cholestenol in the rat. J. Steroid Biochem. 5, 69–72 (1974).
Sun, Y., Lin, Y., Cao, X., Xiang, L. & Qi, J. Sterols from Mytilidae show anti-aging and neuroprotective effects via anti-oxidative activity. Int. J. Mol. Sci. 15, 21660–21673 (2014).
Barbier, M. Isolation of 24-methylenecholesterol-derived oxidation products from queen honeybee ovaries (Apis mellifica L.). J. Chem. Ecol. 13, 1681–1687 (1987).
Vanderplanck, M. et al. How does pollen chemistry impact development and feeding behaviour of polylectic bees? PLoS ONE 9, e86209 (2014).
Fontana, R. et al. Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera). Peptides 25, 919–928 (2004).
Shen, L. et al. Expression of recombinant AccMRJP1 protein from royal jelly of Chinese honeybee in Pichia pastoris and its proliferation activity in an insect cell line. J. Agric. Food Chem. 58, 9190–9197 (2010).
Otwinowski, Z. & Minor, W.  Processing of X-ray diffraction data collected in oscillation mode. in Methods in Enzymology, Vol. 276 (ed. Charles W. Carter, Jr.) 307–326 (Academic Press, 1997).
Karplus, P. A. & Diederichs, K. Assessing and maximizing data quality in macromolecular crystallography. Curr. Opin. Struct. Biol. 34, 60–68 (2015).
Long, F., Vagin, A. A., Young, P. & Murshudov, G. N. BALBES: a molecular-replacement pipeline. Acta Crystallogr. D Biol. Crystallogr. 64, 125–132 (2008).
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 66, 486–501 (2010).
Wu, L. J., Zhang, T., Gu, Y. X., Zheng, C. D. & Fan, H. F. Direct-method SAD phasing of proteins enhanced by the use of intrinsic bimodal phase distributions in the subsequent phase-improvement process. Acta Crystallogr. D Biol. Crystallogr. 65, 1213–1216 (2009).
Brunger, A. T. Version 1.2 of the Crystallography and NMR system. Nat. Protoc. 2, 2728–2733 (2007).
Winn, M. D. et al. Overview of the CCP4 suite and current developments. Acta Crystallogr. D Biol. Crystallogr. 67, 235–242 (2011).
Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, (213–221 (2010).
Schrödinger, L. The PyMOL Molecular Graphics System,Version 1.3. (2010).
Baker, N. A., Sept, D., Joseph, S., Holst, M. J. & McCammon, J. A. Electrostatics of nanosystems: application to microtubules and the ribosome. Proc. Natl. Acad. Sci. USA 98, 10037–10041 (2001).
Schuck, P. Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling. Biophys. J. 78, 1606–1619 (2000).
Zhang, X. et al. Complex structures of the abscisic acid receptor PYL3/RCAR13 reveal a unique regulatory mechanism. Structure 20, 780–790 (2012).
Hammersley, A. P., Svensson, S. O., Hanfland, M., Fitch, A. N. & Hausermann, D. Two-dimensional detector software: From real detector to idealised image or two-theta scan. High Press. Res. 14, 235–248 (1996).
Sreerama, N. & Woody, R. W. Estimation of protein secondary structure from circular dichroism spectra: comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set. Anal. Biochem. 287, 252–260 (2000).
Yu, S., Ye, X., Chen, L., Lian, X. Y. & Zhang, Z. Polyoxygenated 24,28-epoxyergosterols inhibiting the proliferation of glioma cells from sea anemone Anthopleura midori. Steroids 88, 19–25 (2014).
Wallace, A. C., Laskowski, R. A. & Thornton, J. M. LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions. Protein Eng. 8, 127–134 (1995).
We thank Prof. Jiafu Long for help with analytical ultracentrifugation experiments. We thank Prof. Hening Lin and Prof. Peng Gong for critical reading. We would like to thank the staff at the beamlines BL17U, BL19U1, and BL19U2 at Shanghai Synchrotron Radiation Facility, beamline BL5A of at Photon Factory at Tsukuba and the beamlines 1W2A and 4B8 at the Beijing Synchrotron Radiation Facility for the excellent technical assistance during data collection. This work was supported financially by the National Natural Science Foundation of China (31570725, 31370720, 91519332, and 31502022), National Key Research and Development Program of China (2016YFC1200400), Extramural Scientists of State Key Laboratory of Agrobiotechnology Grant (2018SKLAB6-1), the Agricultural Science and Technology Innovation Program, China (ASTIP) and the Building of Modern Agricultural Industry (Bees) R&D Systems in China (NYCYTI-43-KXJ17).
The authors declare no competing interests.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Tian, W., Li, M., Guo, H. et al. Architecture of the native major royal jelly protein 1 oligomer. Nat Commun 9, 3373 (2018). https://doi.org/10.1038/s41467-018-05619-1
This article is cited by
Structure of native glycolipoprotein filaments in honeybee royal jelly
Nature Communications (2020)
The nuclear and mitochondrial genomes of Frieseomelitta varia – a highly eusocial stingless bee (Meliponini) with a permanently sterile worker caste
BMC Genomics (2020)
pH-dependent stability of honey bee (Apis mellifera) major royal jelly proteins
Scientific Reports (2019)
Royal jelly in focus
Insectes Sociaux (2019)
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.