Correspondence | Open | Published:

# Antipsychotics with similar association kinetics at dopamine D2 receptors differ in extrapyramidal side-effects

## Introduction

The recent publication in Nature Communications by Sykes et al.1 made the interesting observation that forward binding rate constants (kon) at the dopamine D2 receptor (D2R) differ widely between antipsychotics, and moreover, that kon rather than koff (dissociation rate constant) values correlate with their liabilities to produce extrapyramidal symptoms (EPS). A high kon is postulated to cause a high degree of rebinding of the antipsychotic to D2R, conferring increased competition with endogenous dopamine, and thereby more EPS. As pointed out by the authors, their results are largely in agreement with our previous findings from a potassium channel (G protein-activated inward rectifier potassium; GIRK) activation assay2. However, there are some discrepancies between their work and ours which may have important consequences for the interpretation of the correlation between kon and EPS, and which we would like to point out below.

Remoxipride, a clinically effective antipsychotic which was withdrawn due to the occurrence of aplastic anemia as a rare side-effect, has a very low rate of EPS compared to the typical antipsychotic, haloperidol3 (odds ratio for rigidity, 0.30; 95% CI, 0.22 to 0.41; n = 1104; P < 0.0001, Fisher’s exact test; Fig. 1). Importantly, unlike many other antipsychotics which target multiple types of monoamine receptors, both of these compounds preferentially bind to dopamine D2-like receptors4. Thus, the difference in EPS liability between remoxipride and haloperidol cannot easily be attributed to differential engagement of muscarinic or serotonergic receptors, which are thought to contribute to the low EPS potential of many of the newer antipsychotics, such as clozapine, quetiapine, and olanzapine4,5,6. This should make remoxipride and haloperidol particularly suitable for comparison based on their kon values at D2R. Interestingly, in our hands, remoxipride has a high kon, similar to that of haloperidol2 (Fig. 1), and a very rapid koff, more than 5 times faster than that of clozapine. In the study by Sykes et al.1, however, remoxipride kon is among the lowest in their dataset, thus fitting their kon hypothesis, while they report a koff for remoxipride which is similar to that of clozapine. It should be mentioned that remoxipride does not induce direct blockade of potassium currents up to a concentration of at least 300 µM, thus ruling out a potentially confounding effect of direct GIRK channel block on the measurement of D2R binding kinetics in our assay7.

Plotting our own kon data against EPS odds ratios taken from Leucht et al.8 yields a similar positive correlation (Fig. 1) as that reported by Sykes et al.1 However, considering the kinetic properties of remoxipride found in our study, along with its low EPS liability, the correlation between kon and said side-effects becomes less striking. We have inserted data for remoxipride vs. placebo (odds ratio, 1.43; 95% CI, 0.96 to 2.13; see Methods), which was not included in the data set presented by Leucht et al.8, based on the odds ratio for rigidity given above. When analyzing data for the 9 compounds which were included both in our study2 and that of Leucht et al.8, the correlation is strong (Spearman’s Rs = 0.93; P < 0.001), but is weakened by the inclusion of remoxipride (Spearman’s Rs = 0.70; P = 0.03).

The reasons for the discrepancies in remoxipride kon and koff between our study2 and that of Sykes et al.1 may relate to the differential kinetics of the probe ligands employed in the two studies–as pointed out in a previous publication by the same authors9, the kinetics of fast-dissociating ligands cannot by accurately measured by a much slower-dissociating probe ligand. It is thus relevant to note that the dissociation T1/2 of PPHT-red, the fluorescent ligand used to probe receptor-ligand interactions, is about 80 s (koff = 0.52 min−1)1, whereas the kinetics of the dopamine response in our potassium channel assay is considerable faster, with a deactivation T1/2 of just below 3 s and an activation T1/2 below 1 s10. For comparison, the T1/2 which we measured for remoxipride was 3.4 s2, whereas Sykes et al.1 reported it as 0.36 min (21.6 s). Thus, estimates of the very rapid binding kinetics of remoxipride may have been distorted by the relatively slow kinetics of PPHT-red. In addition, the differences in kinetic parameters measured for remoxipride may also reflect the differences in receptor state–whereas our assay measures the functional, high-affinity state of D2R in living cells, the study by Sykes et al.1 used GppNHp to convert all D2R in their membrane preparation to the non-signaling, low-affinity state. We consider that drug interactions with the functional state of the receptor might be more relevant to the occurrence of side-effects. Whereas dopamine binding to D2R is known to be sensitive to the transmembrane voltage, such that the EC50 for dopamine-induced GIRK activation is about 4.2-fold higher at 0 mV compared to −80 mV10, we found the IC50 of remoxipride to inhibit the GIRK response to 100 nM dopamine to be 4.8-fold lower at 0 mV (IC50 202.2 nM; pIC50 6.69 ± 0.11; n = 5) compared to −80 mV (IC50 963.5 nM; pIC50 6.02 ± 0.09; n = 5). The IC50 value at −80 mV has been published previously7. This would be consistent with a competitive interaction between dopamine and remoxipride, where the affinity of remoxipride itself is not substantially altered by transmembrane voltage. Thus, a difference in remoxipride binding kinetics related to differential membrane potentials (the transmembrane voltage across isolated membranes, as used by Sykes et al.1, would be 0 mV, while the whole cells used in our study were clamped at a more physiological −80 mV2), would seem unlikely, although a reciprocal change in both kon and koff cannot be ruled out. Lastly, we note that thioridazine, which had been placed in the uncertain typical/atypical category1, has among the highest kon values reported by Sykes et al.1, yet it has consistently been reported to have a favorable profile in terms of EPS11.

In summary, while we find the concept of rebinding, as discussed by Sykes et al.1, to be of great interest and of potential utility to the field of neuropharmacology, we believe that kon is not the only factor governing EPS liability, even when considering D2R-preferring compounds. This is illustrated by comparing haloperidol and remoxipride, which despite having similar kon at D2R in our hands, show very distinct EPS profiles in the clinic.

## Methods

### Electrophysiology data acquisition

The IC50 of remoxipride at −80 and 0 mV was determined using two-electrode voltage clamp in Xenopus oocytes expressing the human D2R together with RGS-4 and GIRK1/4 channels, measuring the GIRK current amplitude as a readout of D2R occupancy by dopamine2, 7, 10. For concentration-response data, oocytes were randomly clamped at either −80 or 0 mV and exposed first to 100 nM dopamine, to evoke GIRK activation. Thereafter, four increasing concentrations (10 nM–10 µM) of remoxipride were applied consecutively, in the continued presence of 100 nM dopamine, at 50-s intervals. The current amplitude at the end of each antagonist application interval was normalized to the control response to 100 nM dopamine, within every oocyte. kon values were also obtained using the GIRK assay, and were taken from our previous work2. Oocyte harvesting from female Xenopus laevis toads was performed in accordance with the Guide for Care and Use of Laboratory Animals as adopted and promulgated by the United States National Institutes of Health. Prior approval for the procedure was granted by the Swedish National Board for Laboratory Animals and the local ethics committee; Stockholms Norra Djurförsöksetiska nämnd.

### Odds ratio calculation

The odds ratio for remoxipride vs. haloperidol was obtained from EPS (rigidity) incidences and number of patients reported by Lewander et al.3 The odds ratio for remoxipride vs. placebo was calculated as

$${\mathrm{OR}}\left( {R{\mathrm{|}}P} \right) = \frac{{R_{{\mathrm{EPS}} + }/R_{{\mathrm{EPS}} - }}}{{P_{{\mathrm{EPS}} + }/P_{{\mathrm{EPS}} - }}} =$$
(1)
$$\left( {\frac{{R_{{\mathrm{EPS}} + }/R_{{\mathrm{EPS}} - }}}{{H_{{\mathrm{EPS}} + }/H_{{\mathrm{EPS}} - }}}} \right) \times \left( {\frac{{H_{{\mathrm{EPS}} + }/H_{{\mathrm{EPS}} - }}}{{P_{{\mathrm{EPS}} + }/P_{{\mathrm{EPS}} - }}}} \right) = {\mathrm{OR}}\left( {R{\mathrm{|}}H} \right) \times {\mathrm{OR}}\left( {H{\mathrm{|}}P} \right)$$

where $$R_{{\mathrm{EPS}} \pm }$$, $$H_{{\mathrm{EPS}} \pm }$$, and $$P_{{\mathrm{EPS}} \pm }$$ denote number of patients treated with remoxipride, haloperidol, and placebo, with our with or without EPS, respectively. Given the relationship above (1), the standard error $${\mathrm{SE}}_{\log {\mathrm{OR}}\left( {R|P} \right)}$$ propagates according to

$${\mathrm{SE}}_{\log {\mathrm{OR}}\left( {R|P} \right)} = \sqrt {{\mathrm{SE}}_{\log {\mathrm{OR}}\left( {R|H} \right)}^2 + {\mathrm{SE}}_{\log {\mathrm{OR}}\left( {H|P} \right)}^2}$$
(2)

which was used to calculate the confidence interval for remoxipride. This extension of the data set to include remoxipride did not use the Bayesian hierarchical model employed by Leucht et al.8 However, the odds ratio calculated here should provide a good estimate for comparison with the antipsychotics in the original analysis.

### Statistical analysis

The statistical significance of the differential proportions of patients experiencing EPS between haloperidol-treated and remoxipride-treated subjects (data from Lewander et al.3) was calculated using Fisher’s exact test.

### Data availability

All relevant data are available from the authors upon request.

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

## References

1. 1.

Sykes, D. A. et al. Extrapyramidal side effects of antipsychotics are linked to their association kinetics at dopamine D2 receptors. Nat. Commun. 8, 763 (2017).

2. 2.

Sahlholm, K. et al. The fast-off hypothesis revisited: A functional kinetic study of antipsychotic antagonism of the dopamine D2 receptor. Eur. Neuropsychopharmacol. 26, 467–476 (2016).

3. 3.

Lewander, T., Westerbergh, S. E. & Morrison, D. Clinical profile of remoxipride--a combined analysis of a comparative double-blind multicentre trial programme. Acta Psychiatr. Scand. Suppl. 358, 92–98 (1990).

4. 4.

Bymaster, F. P. et al. Radioreceptor binding profile of the atypical antipsychotic olanzapine. Neuropsychopharmacology 14, 87–96 (1996).

5. 5.

Bymaster, F. P. et al. Muscarinic mechanisms of antipsychotic atypicality. Prog. Neuropsychopharmacol. Biol. Psychiatry 27, 1125–1143 (2003).

6. 6.

Meltzer, H. Y., Massey, B. W. & Horiguchi, M. Serotonin receptors as targets for drugs useful to treat psychosis and cognitive impairment in schizophrenia. Curr. Pharm. Biotechnol. 13, 1572–1586 (2012).

7. 7.

Sahlholm, K. et al. Typical and atypical antipsychotics do not differ markedly in their reversibility of antagonism of the dopamine D2 receptor. Int. J. Neuropsychopharmacol. 17, 149–155 (2014).

8. 8.

Leucht, S. et al. Comparative efficacy and tolerability of 15 antipsychotic drugs in schizophrenia: a multiple-treatments meta-analysis. Lancet 382, 951–962 (2013).

9. 9.

Klein Herenbrink, C. et al. The role of kinetic context in apparent biased agonism at GPCRs. Nat. Commun. 7, 10842 (2016).

10. 10.

Sahlholm, K. The role of RGS protein in agonist-dependent relaxation of GIRK currents in Xenopus oocytes. Biochem. Biophys. Res. Commun. 415, 509–514 (2011).

11. 11.

Fenton, M., Rathbone, J., Reilly, J. & Sultana, A. Thioridazine for schizophrenia. Cochrane Database Syst. Rev. CD001944, https://doi.org/10.1002/14651858.CD001944.pub2 (2007).

## Author information

H.Z. performed the mathematical analysis. K.S. drafted the first version of the manuscript. Both authors jointly conceived of the idea for the paper and prepared the final version of the manuscript.

### Competing interests

The authors declare no competing interests.

Correspondence to Kristoffer Sahlholm.

## Rights and permissions

Reprints and Permissions

• ### Reply to âAntipsychotics with similar association kinetics at dopamine D2 receptors differ in extrapyramidal side-effectsâ

• David A. Sykes
• , J. Robert Lane
• , Monika Szabo
• , Ben Capuano
• , Jonathan A. Javitch
•  & Steven J. Charlton

Nature Communications (2018)