The conserved decapping enzyme Dcp2 recognizes and removes the 5′ eukaryotic cap from mRNA transcripts in a critical step of many cellular RNA decay pathways. Dcp2 is a dynamic enzyme that functions in concert with the essential activator Dcp1 and a diverse set of coactivators to selectively and efficiently decap target mRNAs in the cell. Here we present a 2.84 Å crystal structure of K. lactis Dcp1–Dcp2 in complex with coactivators Edc1 and Edc3, and with substrate analog bound to the Dcp2 active site. Our structure shows how Dcp2 recognizes cap substrate in the catalytically active conformation of the enzyme, and how coactivator Edc1 forms a three-way interface that bridges the domains of Dcp2 to consolidate the active conformation. Kinetic data reveal Dcp2 has selectivity for the first transcribed nucleotide during the catalytic step. The heterotetrameric Edc1–Dcp1–Dcp2–Edc3 structure shows how coactivators Edc1 and Edc3 can act simultaneously to activate decapping catalysis.
Degradation of mRNA transcripts plays a critical role in maintenance of the cellular transcriptome and in control of eukaryotic gene expression1. Removal of the m7GpppN cap structure found at the 5′ end of all eukaryotic mRNA marks these transcripts for rapid clearance via the 5′-to-3′ mRNA decay pathway2. The conserved mRNA-decapping enzyme Dcp2 catalyzes hydrolysis of the 5′ cap to liberate m7GDP and 5′ monophosphate RNA, which can then be degraded by conserved 5′-3′ exonucleases3,4,5,6. Dcp2-mediated decapping is a critical step in many cellular RNA decay pathways including bulk 5′–3′ decay4,5, nonsense-mediated decay7,8,9,10, microRNA-mediated decay11,12, decay of long-noncoding RNA13, and decay of transcripts containing non-optimal codons14. Recently, Dcp2 was also shown to be a reader of reversible m6A modifications in the 5′ cap, whereby m6A methylation inhibits decapping and stabilizes cellular mRNA transcripts15.
Dcp2 consists of an N-terminal regulatory domain (NRD) and a catalytic Nudix domain (CD)16,17 linked by a short, flexible hinge that allows the enzyme to dynamically adopt different conformations in solution18,19 and in crystal structures20,21,22,23,24. The CD harbors a positively charged patch for RNA binding (box B motif)25,26 and a Nudix motif with conserved Glu residues that bind magnesium and carry out cap hydrolysis chemistry27,28. Fungal Dcp2 also contains an unstructured C-terminal extension that harbors protein–protein interaction motifs and elements that inhibit Dcp2 catalysis29. At least some of these motifs are transferred to the C-terminus of the essential activator Dcp1 in metazoans, suggesting a conserved biological function30. The NRD enhances decapping activity by specifically recognizing the m7G nucleotide of cap and binding Dcp1, which further accelerates decapping17,19,21,22,31,32.
The activity of the core Dcp1–Dcp2 complex can be further modulated by the enhancer of decapping proteins (Edcs)33. For example, the Edc1-type activators, which include the yeast paralog Edc2 and PNRC2 in metazoans, contain conserved tandem short linear motifs to bind Dcp1 and activate Dcp2 catalysis19,20,21,34,35,36,37. Edc3 activates decapping by promoting RNA binding and alleviating autoinhibition of Dcp2 in yeast22,29,38,39,40,41,42. Edc3 is recruited to the decapping complex via short linear motifs found in the fungal or metazoan Dcp2 or Dcp1 C-terminal extension, respectively29,33. Edc4 is a metazoan-specific scaffolding protein that strengthens Dcp1–Dcp2 interaction and coordinates decapping with exonucleolytic degradation by Xrn132,43,44,45. Genetic and physical interactions between activator proteins suggest these cofactors work together to promote decapping, but how this is achieved at the structural level is unknown45,46,47,48,49.
Several recent structural studies have provided new insights into how Dcp2 recognizes the m7G cap, how Dcp2 activity is enhanced by coactivators, and how Dcp2 solution conformations are tied to catalysis. First, a study by Valkov et al. presented a structure of S. pombe Edc1–Dcp1–Dcp2 in which the activation motif in Edc1 bridges the Dcp2 NRD and CD to apparently promote a conformation of Dcp2 that enhances RNA binding20. Soon after, our lab published the first substrate analog-bound structure of S. pombe Dcp1–Dcp2 in complex with human PNRC221. Our structure showed a very different conformation than the Valkov structure, in which the substrate analog was bound by a composite binding site using conserved residues on the NRD and CD. However, in this pre-catalytic conformation of Dcp2 the catalytic Glu on the Nudix helix was positioned too far from substrate for effective catalysis, and the activation motif of PNRC2 was disordered, suggesting an additional conformational change in Dcp2 was needed to achieve catalysis. Simultaneously, Charenton et al. published a K. lactis Dcp1–Dcp2–Edc3 structure with bound m7GDP product in which m7G was recognized by the Dcp2 NRD as in our Mugridge et al. substrate-bound structure, but the CD was rotated by ~90° to bring the Nudix helix much closer to the cleaved m7GDP product, consistent with an active conformation of Dcp222. Finally, most recently Wurm et al. published a study that presented an S. pombe Edc1–Dcp1–Dcp2 product-bound structure in the same conformation as observed by Charenton et al., a conformation that is significantly populated in solution when Edc1 and m7GDP or capped RNA are bound19.
In this study, we present a new, four-protein structure of K. lactis Edc1–Dcp1–Dcp2–Edc3 at 2.84 Å resolution in the catalytically active conformation with bound substrate analog poised for catalysis. We show that the conserved YAGxxF activation motif of Edc1 mediates a three-way interface between Dcp1 and the Dcp2 NRD and CD, and that lesions on the NRD and in the flexible hinge linking the CD and NRD abolish activation by Edc1. Substrate analog binding to the active conformation of Dcp2 suggests that the first transcribed nucleotide of RNA is recognized by base stacking to a conserved aromatic residue at the top of the RNA-binding channel, and that the RNA body follows this positively charged path to bound Edc3 coactivator. We show that Dcp2 has selectivity for the first transcribed nucleotide of RNA substrate and that this selectivity depends on the conserved aromatic residue that binds this nucleotide of our substrate analog. Overall, these data show how substrate binds and is recognized by the catalytically active conformation of Dcp2, suggest a path for RNA binding consistent with prior in vitro mutagenesis and binding data, and show how coactivators Edc1 and Edc3 can simultaneously bind Dcp2 to enhance mRNA decapping by the Dcp1–Dcp2 complex. Our structure establishes that the active conformation of Dcp2 is the same for both substrate and product-bound complexes, and clarifies the mechanisms of decapping catalysis and control of decapping activation by multiple protein interactions.
Structure of substrate analog-bound Edc1–Dcp1–Dcp2–Edc3
To obtain our recently published substrate analog-bound PNRC2–Dcp1–Dcp2 structure21, we crystallized an apo PNRC2–Dcp1–Dcp2 complex and soaked in cap analog to induce a conformational change and substrate binding within the crystal. While this substrate-bound conformation gave new insight into cap recognition by Dcp2, it was clear that this did not represent the catalytically active conformation because the catalytic Glu residue on the Nudix helix was positioned to too far from bound substrate to carry out hydrolysis chemistry. When we attempted to push Dcp2 into the active conformation by soaking in catalytically required magnesium along with substrate analog, the crystals were physically damaged and diffraction was completely destroyed, suggesting this induced an additional conformational change in Dcp2 that could not be accommodated by the crystal lattice.
To trap the substrate-bound active conformation, we screened different Dcp1–Dcp2 constructs (S. pombe, K. lactis, H. sapiens) in which the catalytic Glu was mutated to Gln to prevent cap hydrolysis for co-crystallization with magnesium, a tight-binding two-headed cap analog50, and coactivators Edc1 and Edc3 (Fig. 1a,b). The K. lactis constructs crystallized most readily and we obtained a structure of Kl Edc1–Dcp1–Dcp2–Edc3 with bound magnesium and two-headed cap analog at 2.84 Å resolution (Fig. 1c, Table 1, solved by molecular replacement with PDB 5LOP22 Dcp1–Dcp2). Dcp2 is in the catalytically active conformation, very similar to that found in recently reported m7GDP product-bound structures (Supplementary Fig. 1; Dcp2 core backbone RMSD is 1.2 Å with PDB 5LOP and 1.9 Å with PDB 5N2V)19,22, with the Nudix helix positioned close to bound cap. Edc3 Lsm domain binds an extended helix at the C-terminus of the Dcp2 CD, as in the recent Kl Dcp1–Dcp2–Edc3 structure22, and the fully ordered Edc1 activation peptide bridges Dcp1 and Dcp2, similar to the recent Sp Edc1–Dcp1–Dcp2 structure19. The co-occupancy of Edc1 and Edc3 coactivators in our structure highlights the idea that these decapping factors work together to promote decapping by complementary mechanisms42.
Edc1 consolidates the active conformation of Dcp2
Edc1-like coactivator proteins contain tandem, conserved short linear motifs for Dcp1 binding and Dcp2 activation (Fig.1b)20,21,35,37. Several structures have now been published that show how the proline-rich Dcp1-binding motif in Edc1-like peptides binds a conserved aromatic cleft on Dcp119,20,21,36, and our structure displays a very similar interaction between Kl Edc1 and Kl Dcp1 as that observed in Sp, Hs, and chimeric Sp–Hs structures (Supplementary Fig. 2). The conserved YAGxxF Dcp2-activating motif creates a 3-way interface that bridges Dcp1, Dcp2 NRD, and Dcp2 CD in the catalytically active conformation by threading through a narrow channel between these domains (Fig. 1d,e). In total, the Edc1 peptide interaction with the Dcp1–Dcp2 complex buries ~1300 Å2 of surface area, with the YAGxxF Dcp2-activing motif accounting for ~500 Å2 of this buried surface. The interface between the Dcp2 CD and Edc1 peptide covers only ~250 Å2 of surface area, consistent with evidence that the peptide alone is insufficient to drive a conformational change in solution19.
We have shown that Edc1-like proteins accelerate decapping catalysis by both increasing kcat and decreasing KM.21,35 Kinetic analysis of the Dcp2-activating YAGxxF motif in PNRC2 showed that mutation of the Tyr residue completely abolishes kcat activation of Dcp2 catalysis, while mutation of Ala and Phe residues significantly impairs activation21. We can now rationalize our previous biochemical observations in the context of the fully activated, substrate analog-bound structure. Tyr359 of Kl Edc1, the residue most critical for activation, makes a cation–π interaction with the strictly conserved Arg39 on the Dcp2 NRD (Fig. 1d). Ala360 packs against conserved hydrophobic residues in the flexible hinge between Dcp2 NRD and CD, and Phe364 plugs into a pocket at the Dcp1–Dcp2 NRD interface consisting of positively charged and hydrophobic residues (Supplementary Fig. 3). These contacts anchor the Edc1 activation motif to Dcp1–Dcp2 and provide a complementary surface for the flexible hinge and 130s loop on the Dcp2 CD to pack against Edc1 in the catalytically active conformation of the enzyme. In this way, protein interactions mediated by the Dcp2 activation motif of Edc1 accelerate kcat not by contacting cap, but instead by limiting torsional degrees of freedom in the hinge to favor the active conformation of Dcp2, in which conserved residues on the CD and NRD recognize and hydrolyze cap using the composite active site.
Residues in Dcp2 NRD and hinge are critical for activation
In order to validate the Edc1–Dcp2 interface observed in our structure and better understand how the Edc1 peptide interacts with Dcp2 to promote catalysis, we mutated conserved residues on the Dcp2 NRD, CD, and hinge (Dcp2 residues shown as sticks in Fig. 1d) that contact YAG residues of the Edc1 peptide, and asked how these mutations affect activation of Dcp2 catalysis by Edc1. Using single-turnover in vitro kinetic decapping assays with cap-radiolabeled RNA substrate and S. pombe Edc1–Dcp1–Dcp2 complex, we find that residues Arg33 (Kl Arg39) and Ile96 (Kl Ile102) are absolutely essential for Edc1 function and that mutation of these conserved residues to Ala or Gly, respectively, completely blocks activation by Edc1 (Fig. 2). In our structure, Arg33 (Kl Arg39) makes a cation–π interaction with Edc1 Tyr359 to anchor the activation motif to Dcp2 NRD, and Ile96 (Kl Ile102) packs against Edc1 Ala360 and the Tyr359 backbone. These data suggest that both positioning of the peptide activation motif along the Dcp1–Dcp2 NRD surface, and specific van der Waals interactions with the Dcp2 hinge that links the NRD and CD, are critically important for Edc1-mediated activation.
Interestingly, mutation of other strictly conserved residues on Dcp2 that contact the Edc1 peptide in the catalytically active conformation, such as Phe36 (Kl Phe42) and Tyr92 (Kl Tyr98), have very little effect on Edc1’s ability to activate Dcp2. Mutation of Tyr220, a distal residue on the CD that is important for conformational dynamics in Dcp242, also has no effect on activation by Edc1. Importantly, none of the tested mutations to conserved Dcp2 residues resulted in large defects in decapping in the absence of Edc1 (Supplementary Fig. 4), showing that Arg33 (Kl Arg39) and Ile96 (Kl Ile102) are critical specifically for Edc1-mediated activation of Dcp2. Finally, in contrast to our previous observations examining Dcp2 activation in the chimeric Hs PNRC2–Sp Dcp1–Dcp2 system where we showed PNRC2 peptides with the YAGxxF Dcp2-activating motif had little impact on the KM of the decapping complex, with native Sp Edc1–Dcp1–Dcp2 complexes we see a ~100-fold decrease in KM along with a ~10-fold increase in kmax upon addition of the activator peptide (Supplementary Fig. 5; Supplementary Table 1). This is consistent with recent evidence that Sp Edc1 peptides can enhance RNA binding to the decapping complex19,20.
Substrate analog binds a composite active site on Dcp2
Two-headed cap substrate analog bound at the Dcp2 active site engages conserved residues on the Dcp2 NRD and CD (Fig. 3a,b). In agreement with our previously reported substrate analog-bound pre-catalytic structure21, and recently reported m7GDP product-bound structures19,22, the m7G of cap is recognized by conserved, catalytically critical Trp and Asp residues on the Dcp2 NRD. The opposite face of this m7G nucleotide packs against the backbone of the Dcp2 CD 190s loop in the catalytically active conformation. This agrees with recent product-bound structures, but differs from the pre-catalytic conformation, in which this face of m7G was engaged in base stacking with Sp Tyr220 (Kl Phe223). In the active conformation reported here however, the CD is rotated ~90° from the pre-catalytic conformation, and Phe223 base stacks with the second m7G of our two-headed substrate analog, likely mimicking binding of the first transcribed nucleotide of RNA substrate to the Dcp2 CD. Additional conserved and catalytically important residues make contacts with the substrate analog phosphate chain, including the general acid Lys134 and Arg132 on the CD, and Lys99 on the NRD.
Substrate analog positioning suggests a path for RNA binding
Our structure suggests that the first transcribed nucleotide of RNA binds Phe223 at the top of an extended, positively charged C-terminal helix on the CD of Dcp2 (Box B helix, Fig. 3a). Based on this cap orientation, the RNA body likely extends down the positively charged Box B helix, past a conserved Lys-rich patch on the dorsal surface of Dcp2, to coactivator Edc3. This predicted RNA-binding path is consistent with many lines of biophysical and biochemical evidence, including: (1) NMR chemical shift perturbations induced by RNA binding on the surface of the Dcp2 CD19,26, (2) biochemical data showing that the Box B helix is critical for decapping activity in vitro25, (3) kinetic analysis showing that mutations to basic residues in both the Box B helix and Lys-rich patch significantly increase substrate KM26, (4) SPR data showing that mutations in the Box B helix significantly weaken RNA binding17, (5) yeast growth assays showing that mutations in the Box B helix and Lys-rich patch result in temperature-sensitive growth defects26, and (6) evidence that Edc3 activates decapping in part by enhancing RNA binding to the decapping complex22. The positioning of substrate analog in the Dcp2 active site revealed by our Edc1–Dcp1–Dcp2–Edc3 structure suggests an RNA-binding path along the Dcp2 Box B helix that nicely agrees with all of this previous RNA binding and activity data, adding structural support to the RNA-binding path that has been hypothesized for some time by ourselves and others.
Substrate and product-bound Dcp2 conformations are similar
Alignment of the Dcp2 catalytic core from the active conformation substrate analog and product-bound structures shows that these adopt overall very similar Dcp2 domain orientations (Fig. 3d; Supplementary Fig. 1a). This is consistent with NMR chemical shift data that suggest the Edc1–Dcp1–Dcp2 complex with either product m7GDP or capped RNA substrate have similar conformations in solution19. The most notable difference in the aligned substrate analog and product-bound Dcp2 structures is the difference in positioning of the phosphate chain of the cap. In the substrate analog-bound state, the β-phosphate, which undergoes nucleophilic attack by water during cap cleavage, is located 5.4 Å from the general base Glu152. This is very similar to the substrate-Glu distance in the prototypical Nudix hydrolase MutT, in which a solution-state NMR structure showed the catalytic Glu residue to be ~6 Å from the non-hydrolyzable AMPCPP substrate, with an intervening water molecule well-positioned for nucleophilic attack of the β-phosphate51. In the post-catalytic, m7GDP product-bound structure, the β-phosphate of cap is positioned nearer to the Nudix helix, at a distance of 4.1 Å from the general base Glu152.
Substrate and product-bound structures also differ in the number of magnesium atoms located at the active site. In our substrate analog-bound structure, we were able to build one magnesium atom that is coordinated by Glu148. Although there is some additional density present in the Fo–Fc difference map at the active site (Supplementary Fig. 6a), this was too weak to reliably build additional Mg2+ or water. This agrees well with a high-resolution structure of the S. cerevisiae Dcp2 CD, which showed a single magnesium atom coordinated directly to Sc Glu149 (Kl Glu148), which is coordinated by other conserved Glu residues through water-mediated contacts. In both the K. lactis and S. pombe product-bound structures, however, three magnesium atoms are placed in the Dcp2 active site, coordinated by the conserved CD Glu residues and the phosphate groups of m7GDP (Supplementary Fig. 6b,c). Enzymology from our lab has shown that the Mg2+-dependence of decapping kinetics fits a hill coefficient of 2.4, suggesting Dcp2 binds at least two metal ions52, which is most consistent with the product-bound structures. In the end, both our substrate analog-bound structure (2.84 Å resolution) and the product-bound structures (3.1 Å and 3.5 Å for PDB 5N2V37 and 5LOP22, respectively) are not at sufficient resolution to very accurately distinguish Mg2+ and water molecules, and high-resolution structures of Dcp2 with bound substrate and product will be required to unambiguously determine the location and precise role of coordinated magnesium and water molecules in the active site.
Dcp2 shows selectivity for the first transcribed nucleotide
As discussed above, our structure of substrate analog-bound Dcp2 suggests that the first transcribed nucleotide of RNA is recognized by the conserved aromatic Phe223 (Sp Tyr220). Surprisingly, although mutation of this residue produces temperature-sensitive growth defects in yeast26, Tyr220Ala mutations in our single-turnover in vitro decapping assays with S. pombe Edc1–Dcp1–Dcp2 or Dcp1–Dcp2 complex had no effect on catalysis (Fig. 2a,b; Supplementary Fig. 4). In the apo, inactive conformation of Dcp1–Dcp2 that predominates in solution19, Tyr220 (Kl Phe223) interacts with conserved aromatic W43 on the NRD of Dcp2 (e.g., PDB 2QKM17, 5J3Y20, 5KQ121), which likely stabilizes the inactive conformation of the enzyme. Furthermore, we have recently shown that Tyr220 plays an important role in mediating Dcp2 conformational dynamics and that mutation of this residue alleviates an autoinhibited conformation of the apo enzyme42. It is therefore likely that Tyr220 is a multi-functional residue, which stabilizes an inactive/autoinhibited conformation of Dcp2 in the apo state and acts as a nucleotide binding platform in the substrate-bound, active conformation. In this way, mutation of Tyr220 may both accelerate catalysis by alleviating autoinhibition and impair catalysis by causing defects in substrate binding, resulting in decapping kinetics that appear relatively unaffected by Tyr220Ala mutation.
It was recently reported that Dcp2-mediated decapping in mammals is inhibited by m6A modification of the first transcribed nucleotide of RNA substrates15, suggesting that Dcp2 may be selective for the identity of the first transcribed nucleotide, as is observed in the bacterial decapping enzyme NudC53. Accordingly, we next asked if Dcp2 selectivity for the first transcribed nucleotide of RNA substrate depends on Tyr220. We compared decapping kinetics for cap-radiolabeled 29-mer RNA substrates where the first transcribed nucleotide was either guanosine (G-RNA), adenosine (A-RNA), or cytosine (C-RNA) using WT, or Tyr220Gly Sp Edc1–Dcp1–Dcp2 decapping complexes (Fig. 4; Supplementary Table 2). Tyr220Gly mutations were used here to ensure maximal disruption of hydrophobic stacking interactions with substrate; this mutation also resulted in a small acceleration of decapping, likely resulting from disruption of the Y220–W43 interaction in the inactive Dcp2 conformation discussed above. With WT decapping complex, kmax for both G-RNA and A-RNA is ~2-fold faster than C-RNA. Upon mutation of Tyr220, decapping is accelerated and the selectivity of 5′ G/A versus C nucleotides is abolished, resulting in approximately the same kmax value for G-RNA, A-RNA, and C-RNA. This suggests that Dcp2 discriminates between G/A versus C at the first transcribed nucleotide position during the catalytic step of decapping, and that this selectivity critically depends on Tyr220 (Kl Phe223). Dcp2′s Tyr220-dependent preference for G/A versus C at this position is in line with the observation that there is a strong genome-wide bias, in both fission yeast54 and humans55, toward purines (G,A) over pyrimidines (C,U) at the transcription start site +1 position. Furthermore, our selectivity data supports the hypothesis that Tyr220 (Kl Phe223) is important for recognition of the first transcribed nucleotide and that the apparent lack of kinetic defects, or even acceleration of decapping, when this residue is mutated may arise from its multi-functional role in different conformations of the enzyme19,42.
Finally, we also asked whether the activated S. pombe decapping complex showed selectivity for A versus m6A at the first transcribed nucleotide position of RNA, as has been recently shown for mammalian Dcp215. In contrast to the mammalian decapping enzyme, we found that fission yeast Edc1–Dcp1–Dcp2 complex, or Dcp2 alone, showed no selectivity for A versus m6A at the 5′-most nucleotide position on a 29-mer RNA substrate in in vitro decapping assays (Supplementary Fig. 7). This suggests that recognition of the m6A modification at the first transcribed nucleotide of mRNA (m6Am in mammals, where this nucleotide is also 2′-O-methylated) by Dcp2 may only be conserved in higher eukaryotes.
Several recent studies have provided new structural information about the mRNA-decapping enzyme Dcp219,20,21,22. These include structures of the Dcp1–Dcp2 heterodimer with markedly different conformations of Dcp2, bound cap analog or m7GDP product, and in complex with Edc1-like or Edc3 coactivators (Fig. 5a). Here we expand on the structural understanding of Dcp2 activation and catalysis with a new structure of the four-protein complex Edc1–Dcp1–Dcp2–Edc3 in the catalytically active conformation of Dcp2 with a tight-binding substrate analog bound to the enzyme active site (Fig. 1a-c, Table 1). The coactivator Edc1 peptide engages conserved residues on Dcp1–Dcp2 NRD, providing a complementary surface against which the Dcp2 CD and flexible hinge pack in the catalytically active conformation, thus consolidating this conformation and promoting catalysis (Fig. 1d,e and 2) Cap substrate analog binds to the enzyme active site in an orientation that positions the RNA body to follow an extended, positively charged helix of Dcp2 to the bound Edc3 coactivator (Fig. 3), which has been shown to enhance RNA binding to these complexes in vitro22. Finally, we show that the conserved aromatic residue Tyr220 (Kl Phe223) that binds the second nucleotide of our substrate analog is critical for recognition of the first transcribed nucleotide in RNA by the decapping complex (Fig. 4). Purine specificity is conferred for this nucleotide during the catalytic step of decapping.
Many different conformations of the decapping enzyme Dcp2 have been structurally characterized, but a consensus is now emerging around the identity of the catalytically active conformation of Dcp2. With the addition of the Edc1–Dcp1–Dcp2–Edc3 cap analog structure presented here, we now have both product m7GDP and substrate analog-bound structures of the Dcp1–Dcp2 decapping complex in what can safely be described as the active conformation (Fig.5a, active structures). The substrate and product-bound active conformations are nearly identical, with major differences only in the positioning of the cap phosphate chain before and after hydrolysis (Fig. 3d). These structures all show that the m7G nucleotide of cap is specifically recognized by stacking with a conserved Trp and hydrogen bonding to a conserved Asp residue on the Dcp2 NRD, explaining why the NRD contributes 100-fold to catalysis31. In the active conformation, the 190s loop of the Dcp2 CD packs against the opposite face of the m7G base, which brings the Nudix helix and catalytic Glu within ~6 Å of the cap β-phosphate, close enough to carry out hydrolysis chemistry and cap cleavage (Fig. 3b).
The catalytic importance and roles of the additional conformations of Dcp2 that have been structurally characterized, but are very different from the active conformation (Fig. 5a, inactive, RNA-binding, and pre-catalytic structures; Fig. 5b,c), remain less clear. In our previously published pre-catalytic substrate analog-bound PNRC2–Dcp1–Dcp2 structure21, Dcp2 adopts a conformation in which the NRD recognizes one m7G nucleotide of cap analog as in the active conformation, but the CD of Dcp2 is rotated 90° and Sp Tyr220 stacks against the opposite face of this m7G, rather than the 190s loop (Fig. 5a, pre-catalytic; Fig. 5c). In the pre-catalytic conformation, the second m7G nucleotide of our two-headed cap analog is bound by the strictly conserved Sp Tyr92 residue near the Dcp2 hinge. In the Edc1-bound active conformation, however, this Tyr is occluded by interaction with the Dcp2-activating motif on Edc1 (Fig. 5d). It is possible that this pre-catalytic conformation, in which cap analog is bound by a composite nucleotide binding site consisting of conserved residues on the Dcp2 CD and NRD, represents an early step in cap recognition that precedes formation of the active conformation and its consolidation by the Edc1 YAGxxF motif. Likewise, the apo Edc1–Dcp1–Dcp2 structure by Valkov et al.20, which adopts a conformation that positions RNA-binding residues on the Dcp2 CD and Dcp1 opposite each other to create a positively charged channel, may represent a conformation of the apo enzyme that is primed for RNA binding or scanning21,24 (Fig. 5a; RNA-binding). With both of these alternative conformations, however, it is so far difficult to rule out the possibility that these are simply off-pathway conformations captured by crystallization. This is not to say that valuable information is not provided by conserved cap or coactivator contacts with the enzyme, but rather that these specific conformations may not be on-pathway in the Dcp2 catalytic cycle. Indeed, recent PRE NMR data suggests that the apo Edc1-bound conformation in the Valkov et al. structure is not populated in solution in vitro19. In these same experiments, it was not possible to distinguish our pre-catalytic substrate-bound conformation from the closed, inactive conformation of Dcp2 that predominates in solution (Fig. 5a, inactive; Fig. 5b).
Building on recent reports of Dcp2 autoinhibition in yeast29, our lab has now shown that the C-terminal extension in fission yeast Dcp2 contains autoinhibitory motifs that interact with the core domains of Dcp1–Dcp2 to inhibit the decapping complex and impair activation by Edc1, and that this autoinhibition is alleviated by coactivator Edc342. The Edc1–Dcp1–Dcp2–Edc3 structure presented here provides the structural framework to begin to understand how coactivators act to alleviate autoinhibition and activate catalysis. We suggest a model of decapping regulation whereby full-length Dcp2 is autoinhibited and coactivators Edc3 and Edc1 act simultaneously to (1) alleviate this autoinhibition, and (2) activate decapping catalysis by both consolidating the Dcp2 active conformation and by enhancing RNA binding to the complex. In this way, switch-like mutli-log unit changes in decapping activity are easily achievable when multiple coactivators bind Dcp2 and function simultaneously. Because many decapping coactivators have genetic or physical interactions with one another29,45,46,47,48,49, we anticipate that combinatorial control of decapping via multiple coactivators is a general and important principle for regulation of mRNA decay.
Protein expression and purification
K. lactis his-tev-Dcp1(1–188)–Dcp2(1–275, E152Q)-Edc3(1–66) polycistronic construct used for crystallization experiments was obtained as a single E. coli codon-optimized DNA sequence with a T7 promoter preceding each protein as a gBlock from Integrated DNA Technologies (his is hexahistadine affinity tag, tev is Tobacco Etch Virus protease cleavage site; see Supplementary Table 3 for sequence). This was cloned into a Kanamycin-resistant expression vector and transformed into E. coli BL21-star DE3 cells (Invitrogen; see Supplementary Table 4 for primers used). Cells were grown in LB media to OD ~0.6, protein expression was induced with IPTG for 18 h at 20 °C. Cells were harvested at 5000 × g, lysed by sonication, and clarified at 16,000 × g in lysis buffer (50 mM sodium phosphate pH 7.5, 300 mM sodium chloride, 10 mM imidazole, 5% glycerol, 10 mM 2-mercaptoethanol, Roche EDTA-free protease inhibitor cocktail). The protein complex was purified by Ni-NTA affinity chromatography and solubility/affinity tags were cleaved by treatment with TEV protease overnight at room temperature in elution buffer (50 mM sodium phosphate pH 7.5, 300 mM sodium chloride, 250 mM imidazole, 10 mM 2-mercaptoethanol). The Kl Dcp1–Dcp2–Edc3 complex was separated from cleaved tags by size exclusion chromatography on a GE Superdex 200 16/60 gel filtration column in crystallization buffer (10 mM HEPES pH 7, 150 mM NaCl, 1 mM DTT). The purified complex was concentrated to 15 mg/mL and flash frozen in liquid nitrogen for crystallization experiments.
S. pombe his-GB1-tev–Dcp1(1–127)–Dcp2(1–243) constructs used for kinetic experiments were obtained by cloning the genomic Sp Dcp2(1–243) coding sequence into MCS2 of Novagen pACYC co-expression vector, and an E. Coli codon-optimized Sp Dcp1(1–127) DNA sequence obtained from Integrated DNA Technologies into MCS1 of the pACYC vector containing an N-terminal his-GB1-tev tag (GB1 is a solubility tag derived from the B1 domain from Protein G; see Supplementary Table 3 for codon-optimized Sp Dcp1 sequence). The Sp Dcp1–Dcp2 protein complex was expressed and purified as described above for the Kl Dcp1–Dcp2–Edc3 complex. After TEV cleavage, the complex was separated from cleaved tags by size exclusion chromatography on a GE Superdex 200 16/60 gel filtration column in storage buffer (50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT). The purified Sp Dcp1–Dcp2 complex was concentrated, glycerol added to a final concentration of 20%, and flash frozen in liquid nitrogen; final concentration was 200 µM. Point mutants of the Sp Dcp1–Dcp2 complex were made by whole plasmid PCR with mutagenic divergent primers, verified by sequencing, and purified as above (see Supplementary Table 4 for primer sequences).
Crystallization of Edc1–Dcp1–Dcp2–Edc3 with substrate analog
Kl Edc1(355-380) peptide (from gene KLLA0_A01474g) was chemically synthesized by Peptide 2.0 and dissolved in crystallization buffer to a concentration of 5 mM. Two-headed cap substrate analog, m7Gp(S)ppp(S)m7G50,56, was dissolved in water at a concentration of 100 mM and the pH was adjusted to ~7.
Protein solution consisting of 4 mg/mL purified K. lactis Dcp1(1–188)–Dcp2(1–275, E152Q)–Edc3(1–66), 1 mM Kl Edc1 peptide, 6 mM substrate analog, and 10 mM MgCl2 was prepared in crystallization buffer and incubated at room temperature for 30 min. Using a TTP Labtech Mosquito Crystal robot, 250 nL of protein solution was combined with 250 nL of well solution, and then 50 nL of seed stock, in a hanging-drop vapor diffusion experiment. Crystallization drops were prepared at room temperature and immediately moved to 4 °C. The well solution contained 220 mM magnesium acetate, 30 mM EDTA, and 8% w/v PEG 8000. The seed stock was prepared using Hampton’s Seed Bead kit, using crystals grown with 1:1 protein solution and well solution containing 200 mM magnesium acetate and 10% w/v PEG 8000 at room temperature, diluted 10,000-fold. Block-shaped crystals ~40–80 µm in size grew at 4 °C within 1–2 days. Crystals were flash frozen in liquid nitrogen using a cryoprotectant consisting of well solution with 15% PEG 8000.
Crystallographic data collection and refinement
All data were collected at Lawrence Berkeley National Lab, Beamline 8.3.1 at the Advanced Light Source on a Dectris Pilatus3 S 6 M detector at 100 K and a wavelength of 1.11583 Å. Data were indexed, integrated and scaled using XDS57. The structures were solved in space group C2 by molecular replacement using Phaser in the CCP4 suite58. Fragments of chains A, B, and C of PDB 5LOP were used as molecular replacement models22, first placing Dcp1–Dcp2 NRD(1–96), then Dcp2 CD(104–240), then Dcp2(252–260)–Edc3(1–66). The asymmetric unit contained two nearly symmetric copies of the Edc1–Dcp1–Dcp2–Edc3 complex. In the crystal lattice, one molecule of Edc3 Lsm domain forms a dimer as in the Kl Edc3–Dcp1–Dcp2–m7GDP product structure (PDB 5LOP)22, but the spacegroups (C2 for AM60 and P6422 for 5LOP) and overall crystal packing are entirely different between our structure and the previously determined K. lactis structure. Molecular replacement failed to correctly place the Dcp2(252–260)–Edc3(1–66) fragment for chain H, as the density was very weak for this chain of Edc3, likely because this molecule of Edc3 does not form a dimer in the crystal lattice. This fragment was placed manually in COOT, based on strong density for the Dcp2(252–260) Edc3-binding motif and strong density for some β-strands in Edc3. After a preliminary round of refinement including rigid-body refinement in PHENIX59, the Edc1 peptide, Dcp2 hinge, and Dcp2 extended helix were built manually in COOT60. The structure was then iteratively refined in PHENIX and manually adjusted in COOT. Final refinement used Non-Crystallographic Symmetry, Translation-Libration-Screw parameterization for chain H, and secondary structure, stereochemical and atomic displacement parameter restraints. For the final model of Kl Edc1–Dcp1–Dcp2–Edc3, all residues were in favored (95.6%) or allowed (4.4%) regions of the Ramachandran plot as evaluated by MolProbity61.
Structural analysis and visualization
All alignments and structural figures were prepared using PyMol version 1.7. Electrostatic surfaces were calculated using PDB2PQR & APBS webservers (http://nbcr-222.ucsd.edu/pdb2pqr_2.0.0/)62,63, using the AMBER94 forcefield to calculate charges. Electrostatic surfaces were visualized in PyMol using APBS tools 2.1. Buried surface area in protein interfaces was calculated using the PDBePISA webserver (http://www.ebi.ac.uk/pdbe/prot_int/pistart.html)64.
Kinetic decapping assays
For the decapping assays measuring kobs for Dcp1–Dcp2 point mutants ±Edc1 described in Fig. 2 and Supplementary Fig. 4, Sp Dcp1–Dcp2(1–243) WT and mutant complexes had a final concentration of 2 µM and Sp Edc1 (155–180) peptide had a final concentration of 100 µM. Sp Edc1 (155–180) peptide was chemically synthesized by Peptide 2.0 and stock solutions were prepared at 5 mM concentration in crystallization buffer, flash frozen in liquid nitrogen, and stored at −20 °C. These experiments used an in vitro transcribed, enzymatically capped 355-mer RNA substrate derived from MFA2 and 32P-labeled at the α-phosphate of cap. Reactions were carried out in the decapping buffer used previously to measure PNRC2 activation kinetics (50 mM HEPES pH 7, 100 mM KCl, 10% glycerol, 5 mM MgCl2, with RNase inhibitor and 0.1 mg/mL acetylated BSA)21. 3× protein solutions with Dcp1–Dcp2+Edc1 peptide, and 1.5× capped RNA solutions were equilibrated separately at 4 °C for 30 min prior to initiation. Reactions were initiated by combining 15 µL 3× protein solution with 30 µL 1.5× RNA solution at 4 °C. Time points were quenched by the addition of excess EDTA, TLC was used to separate RNA from product m7GDP, and the decapped fraction was quantified using a GE Typhoon scanner and ImageQuant software. Plots of fraction m7GDP product versus time were fit to a first order exponential to obtain kobs; in the case of R33A and I96G point mutants, decapping kinetics were too slow to obtain reliable exponential fits and kobs was obtained from a linear fit of initial rates (less than 20% decapped), by dividing the slope by the average endpoint of 0.95. Errors on individual time points in Fig. 2a are the s.d. between two independent repetitions of the decapping reaction; errors on kobs in Fig. 2b are s.d. of kobs values obtained from these two independent replicates.
For decapping assays measuring kinetics with G versus C at the first transcribed nucleotide described in Fig. 4 and those measuring KM and kmax for Edc1 activation (Supplementary Fig. 5, Supplementary Table 1), synthetic 5′-triphosphate 29-mer RNA oligonucleotides with either guanosine (G-RNA) or cytosine (C-RNA) as the 5′ nucleotide were obtained from Trilink BioTechnologies and enzymatically capped with 32P-radiolabeled GTP, as above. In the decapping reaction, Sp Dcp1–Dcp2(1–243) had a final concentration ranging from 2 µM to 4 nM, the final concentration of Sp Edc1(155–180) peptide was held constant at 50 µM, and capped RNA concentration was <100 pM. For these experiments, glycerol was removed from the decapping buffer described above in order to obtain more easily saturable kinetics with the 29-mer RNA substrate. Decapping reactions were prepared, executed, and analyzed as described above. To obtain kmax and KM, kobs was plotted versus protein concentration and fit to the model: kobs = kmax[E]/KM + [E]. Errors on kobs values shown in Fig. 4a are s.d. of two independent replicates. Errors on kmax values shown in Fig. 4b are the standard error from fits to determine kmax and KM.
Coordinates and structure factors were deposited in the Protein Data Bank with accession code PDB 6AM0. Other data are available from the corresponding author upon reasonable request.
Moore, M. J. From birth to death: the complex lives of eukaryotic mRNAs. Science 309, 1514–1518 (2005).
Parker, R. RNA degradation in Saccharomyces cerevisae. Genetics 191, 671–702 (2012).
Dunckley, T. & Parker, R. The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif. EMBO J. 18, 5411–5422 (1999).
Arribas-Layton, M., Wu, D., Lykke-Andersen, J. & Song, H. Structural and functional control of the eukaryotic mRNA decapping machinery. Biochim. Biophys. Acta 580–589, 2013 (1829).
Li, Y. & Kiledjian, M. Regulation of mRNA decapping. Wiley Interdiscip. Rev. 1, 253–265 (2010).
Nagarajan, V. K., Jones, C. I., Newbury, S. F. & Green, P. J. XRN 5′ → 3′ exoribonucleases: structure, mechanisms and functions. Biochim. Biophys. Acta 1829, 590–603 (2013).
Kervestin, S. & Jacobson, A. NMD: a multifaceted response to premature translational termination. Nat. Rev. Mol. Cell Biol. 13, 700–712 (2012).
Popp, M. W.-L. & Maquat, L. E. Organizing principles of mammalian nonsense-mediated mRNA decay. Annu. Rev. Genet. 47, 139–165 (2013).
Cho, H., Kim, K. M. & Kim, Y. K. Human proline-rich nuclear receptor coregulatory protein 2 mediates an interaction between mRNA surveillance machinery and decapping complex. Mol. Cell 33, 75–86 (2009).
Karousis, E. D., Nasif, S. & Mühlemann, O. Nonsense-mediated mRNA decay: novel mechanistic insights and biological impact. Wiley Interdiscip. Rev. RNA 7, 661–682 (2016).
Guo, H., Ingolia, N. T., Weissman, J. S. & Bartel, D. P. Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature 466, 835–840 (2010).
Jonas, S. & Izaurralde, E. Towards a molecular understanding of microRNA-mediated gene silencing. Nat. Rev. Genet. 16, 421–433 (2015).
Geisler, S., Lojek, L., Khalil, A. M., Baker, K. E. & Coller, J. Decapping of long non-coding RNAs regulates inducible genes. Mol. Cell 45, 279–291 (2012).
Presnyak, V. et al. Codon optimality is a major determinant of mRNA stability. Cell 160, 1111–1124 (2015).
Mauer, J. et al. Reversible methylation of m6Am in the 5′ cap controls mRNA stability. Nature 541, 371–375 (2017).
She, M. et al. Crystal structure and functional analysis of Dcp2p from Schizosaccharomyces pombe. Nat. Struct. Mol. Biol. 13, 63–70 (2006).
She, M. et al. Structural basis of Dcp2 recognition and activation by Dcp1. Mol. Cell 29, 337–349 (2008).
Floor, S. N., Borja, M. S. & Gross, J. D. Interdomain dynamics and coactivation of the mRNA decapping enzyme Dcp2 are mediated by a gatekeeper tryptophan. Proc. Natl. Acad. Sci. USA 109, 2872–2877 (2012).
Wurm, J. P., Holdermann, I., Overbeck, J. H., Mayer, P. H. O. & Sprangers, R. Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme. Proc. Natl. Acad. Sci. USA 114, 6034–6039 (2017).
Valkov, E. et al. Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Nat. Struct. Mol. Biol. 23, 574–579 (2016).
Mugridge, J. S., Ziemniak, M., Jemielity, J. & Gross, J. D. Structural basis of mRNA-cap recognition by Dcp1-Dcp2. Nat. Struct. Mol. Biol. 23, 987–994 (2016).
Charenton, C. et al. Structure of the active form of Dcp1-Dcp2 decapping enzyme bound to m7GDP and its Edc3 activator. Nat. Struct. Mol. Biol. 23, 982–986 (2016).
Coller, J. mRNA decapping in 3D. Nat. Struct. Mol. Biol. 23, 954 (2016).
Valkov, E., Jonas, S. & Weichenrieder, O. Mille viae in eukaryotic mRNA decapping. Curr. Opin. Struct. Biol. 47, 40–51 (2017).
Piccirillo, C., Khanna, R. & Kiledjian, M. Functional characterization of the mammalian mRNA decapping enzyme hDcp2. RNA 9, 1138–1147 (2003).
Deshmukh, M. V. et al. mRNA decapping is promoted by an RNA-binding channel in Dcp2. Mol. Cell 29, 324–336 (2008).
Aglietti, R. A., Floor, S. N., McClendon, C. L., Jacobson, M. P. & Gross, J. D. Active site conformational dynamics are coupled to catalysis in the mRNA decapping enzyme Dcp2. Structure 21, 1571–1580 (2013).
Mildvan, A. S. et al. Structures and mechanisms of Nudix hydrolases. Arch. Biochem. Biophys. 433, 129–143 (2005).
He, F. & Jacobson, A. Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain. RNA 21, 1633–1647 (2015).
Fromm, S. A. et al. The structural basis of Edc3‐ and Scd6‐mediated activation of the Dcp1:Dcp2 mRNA decapping complex. EMBO J. 31, 279–290 (2012).
Floor, S. N., Jones, B. N., Hernandez, G. A. & Gross, J. D. A split active site couples cap recognition by Dcp2 to activation. Nat. Struct. Mol. Biol. 17, 1096–1101 (2010).
Chang, C.-T., Bercovich, N., Loh, B., Jonas, S. & Izaurralde, E. The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1. Nucleic Acids Res. 42, 5217–5233 (2014).
Jonas, S. & Izaurralde, E. The role of disordered protein regions in the assembly of decapping complexes and RNP granules. Genes Dev. 27, 2628–2641 (2013).
Dunckley, T., Tucker, M. & Parker, R. Two related proteins, Edc1p and Edc2p, stimulate mRNA decapping in Saccharomyces cerevisiae. Genetics 157, 27–37 (2001).
Borja, M. S., Piotukh, K., Freund, C. & Gross, J. D. Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition. RNA 17, 278–290 (2011).
Lai, T. et al. Structural basis of the PNRC2-mediated link between mRNA surveillance and decapping. Structure 20, 2025–2037 (2012).
Wurm, J. P., Overbeck, J. & Sprangers, R. The S. pombe mRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface. RNA 22, 1360–1372 (2016).
Kshirsagar, M. & Parker, R. Identification of Edc3p as an enhancer of mRNA decapping in Saccharomyces cerevisiae. Genetics 166, 729–739 (2004).
Badis, G., Saveanu, C., Fromont-Racine, M. & Jacquier, A. Targeted mRNA degradation by deadenylation-independent decapping. Mol. Cell 15, 5–15 (2004).
Dong, S. et al. YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA. Mol. Cell 25, 559–573 (2007).
Harigaya, Y., Jones, B. N., Muhlrad, D., Gross, J. D. & Parker, R. Identification and analysis of the interaction between Edc3 and Dcp2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 30, 1446–1456 (2010).
Paquette, D. R., Tibble, R. W., Daifuku, T. S. & Gross, J. D. Control of mRNA decapping by autoinhibition. Preprint at https://doi.org/10.1101/266718 (2018).
Fenger-Grøn, M., Fillman, C., Norrild, B. & Lykke-Andersen, J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Mol. Cell 20, 905–915 (2005).
Yu, J. H., Yang, W.-H., Gulick, T., Bloch, K. D. & Bloch, D. B. Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body. RNA 11, 1795–1802 (2005).
Eulalio, A. et al. Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing. Genes Dev. 21, 2558–2570 (2007).
Gavin, A.-C. et al. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415, 141–147 (2002).
Krogan, N. J. et al. Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440, 637–643 (2006).
Collins, S. R. et al. Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map. Nature 446, 806–810 (2007).
Decourty, L. et al. Linking functionally related genes by sensitive and quantitative characterization of genetic interaction profiles. Proc. Natl. Acad. Sci. USA 105, 5821–5826 (2008).
Ziemniak, M. et al. Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex. RNA 22, 518–529 (2016).
Lin, J., Abeygunawardana, C., Frick, D. N., Bessman, M. J. & Mildvan, A. S. Solution structure of the quaternary MutT−M2+ −AMPCPP−M2+ complex and mechanism of its pyrophosphohydrolase action. Biochemistry 36, 1199–1211 (1997).
Jones, B. N., Quang-Dang, D.-U., Oku, Y. & Gross, J. D. in Methods in Enzymology (eds Maquat, L.E. and Kiledjian, M.) Vol. 448, 23–40 (Academic Press, Cambridge, Massachusetts, 2008).
Höfer, K. et al. Structure and function of the bacterial decapping enzyme NudC. Nat. Chem. Biol. 12, 730–734 (2016).
Li, H. et al. Genome-wide analysis of core promoter structures in Schizosaccharomyces pombe with DeepCAGE. RNA Biol. 12, 525–537 (2015).
Carninci, P. et al. Genome-wide analysis of mammalian promoter architecture and evolution. Nat. Genet. 38, 626–635 (2006).
Ziemniak, M. et al. Phosphate-modified analogues of m(7)GTP and m(7)Gppppm(7)G-synthesis and biochemical properties. Bioorg. Med. Chem. 23, 5369–5381 (2015).
Kabsch, W. XDS. Acta Crystallogr. D Biol. Crystallogr. 66, 125–132 (2010).
McCoy, A. J. et al. Phaser crystallographic software. J. Appl. Crystallogr. 40, 658–674 (2007).
Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213–221 (2010).
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 66, 486–501 (2010).
Chen, V. B. et al. MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr. D Biol. Crystallogr. 66, 12–21 (2010).
Dolinsky, T. J. et al. PDB2PQR: expanding and upgrading automated preparation of biomolecular structures for molecular simulations. Nucleic Acids Res. 35, W522–W525 (2007).
Baker, N. A., Sept, D., Joseph, S., Holst, M. J. & McCammon, J. A. Electrostatics of nanosystems: application to microtubules and the ribosome. Proc. Natl. Acad. Sci. USA 98, 10037–10041 (2001).
Krissinel, E. & Henrick, K. Inference of macromolecular assemblies from crystalline state. J. Mol. Biol. 372, 774–797 (2007).
We thank J. Holton and G. Meigs at Lawrence Berkeley National Laboratory, Advanced Light Source beamline 8.3.1, and X. Liu for help with X-ray data collection and refinement. We also thank J. Kowalska for helpful discussions on the design and synthesis of the two-headed cap analog. This work was supported by the US National Institutes of Health (R01 GM078360 to J.D.G. and NRSA fellowship F32 GM105313 to J.S.M.), The Foundation for Polish Science (TEAM/2016-2/13 to J.J.), the National Science Centre, Poland (fellowship no. UMO-2014/12/T/NZ1/00528 to M.Z.), the Genentech Foundation Fellowship (to R.W.T), and the UCSF Discovery Fellowship (to R.W.T). The Advanced Light Source is supported by the US Department of Energy under contract no. DE-AC02-05CH11231.
The authors declare no competing interests.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Mugridge, J.S., Tibble, R.W., Ziemniak, M. et al. Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis. Nat Commun 9, 1152 (2018). https://doi.org/10.1038/s41467-018-03536-x
This article is cited by
Nature Chemical Biology (2021)
Nature Structural & Molecular Biology (2018)