Polymyxins remain the last line treatment for multidrug-resistant (MDR) infections. As polymyxins resistance emerges, there is an urgent need to develop effective antimicrobial agents capable of mitigating MDR. Here, we report biodegradable guanidinium-functionalized polycarbonates with a distinctive mechanism that does not induce drug resistance. Unlike conventional antibiotics, repeated use of the polymers does not lead to drug resistance. Transcriptomic analysis of bacteria further supports development of resistance to antibiotics but not to the macromolecules after 30 treatments. Importantly, high in vivo treatment efficacy of the macromolecules is achieved in MDR A. baumannii-, E. coli-, K. pneumoniae-, methicillin-resistant S. aureus-, cecal ligation and puncture-induced polymicrobial peritonitis, and P. aeruginosa lung infection mouse models while remaining non-toxic (e.g., therapeutic index—ED50/LD50: 1473 for A. baumannii infection). These biodegradable synthetic macromolecules have been demonstrated to have broad spectrum in vivo antimicrobial activity, and have excellent potential as systemic antimicrobials against MDR infections.
“ESKAPE” pathogens, including A. baumannii, K. pneumoniae, and P. aeruginosa, are a coterie of bacteria that exhibit a high incidence of antibiotic resistance, and are common causes of hospital-acquired infections especially in immunocompromised and critically ill patients1. Over the past few decades, the emergence of polymyxins resistance in these pathogens has become increasingly prevalent2. Despite tremendous efforts, the panacea for these recalcitrant infections has not been found. With no effective and safe treatments available, multidrug-resistant (MDR) infections are quickly morphing into a global healthcare threat. Polymyxins remain the last line treatment for these MDR Gram-negative bacterial infections although they are associated with nephrotoxicity and neurotoxicity, and are not active against Gram-positive bacteria or fungi3, 4. There is thus a dire need to develop novel antimicrobial compounds that have a broad spectrum of activity against both Gram-positive and -negative bacteria, yet at the same time, are well tolerated with low propensity for resistance development.
To address this problem, an unconventional class of antimicrobial agents comprising of peptides5,6,7 and synthetic polymers have recently emerged8. These peptides/polymers contain cationic charges that target negatively charged microbial membrane (phosphate head groups) through electrostatic interaction and hydrophobic components that disrupt lipid domains of the cytoplasmic membrane, leading to cell death (membrane-disruption antimicrobial mechanism). The balance of cationic charge (hydrophilicity) and hydrophobicity renders selectivity toward microbes as mammalian cell surface is generally neutral9,10,11,12. While exhibiting broad-spectrum antimicrobial activities, antimicrobial peptides possess certain inherent limitations such as high manufacturing costs and in vivo toxicity, which limits their systemic applications in the clinic although they are currently used in topical treatments8, 13. Significant efforts have thus been directed toward the development of synthetic antimicrobial polymers as pioneered by DeGrado and colleagues14, Gellman and colleagues15, Tew and colleagues9, 10, and Kuroda and colleagues16, 17. Most antimicrobial polymers reported in the literature have non-degradable backbones, which may potentially lead to accumulation in the body and long-term toxicity. In addition, the related studies mainly focused on structure–activity relationships through evaluation of in vitro antimicrobial and hemolytic activities. Little research has been performed on in vivo activity of antimicrobial polymers, although one paper by Kuroda’s lab reported that treatment with cationic methacrylate polymers effectively reduced the number of bacteria in a rat nasal Gram-positive S. aureus colonization model18. We recently synthesized biodegradable quaternary ammonium-containing amphiphilic polycarbonates as antimicrobial agents12, 19,20,21,22 using metal-free organocatalytic living ring-opening polymerization (ROP) methodology developed by our group23, 24. This polymerization strategy allows for precise control of molecular length and functionality as well as modulation of their corresponding structure–activity relationships. The polymers with optimal structures were potent against bacteria through the membrane-disruption mechanism, and effective in treating Gram-positive methicillin-resistant S. aureus (MRSA)-caused systemic infection21, 25. However, these polycarbonates are not active against P. aeruginosa, A. baumannii, or K. pneumoniae21. Guanidinium-rich molecules (e.g., HIV-1 TAT peptide) exhibit a membrane-translocation property, which is attributed to the ability of guanidinium to form stable multidentate hydrogen bonds with phosphate or sulfate head groups on the cell membrane26,27,28,29,30. Guanidinium-functionalized non-degradable polynorbornenes31 and polymethacrylates32 were reported to be more potent against bacteria in vitro than their amine-functionalized counterparts, and had significantly less interaction with serum proteins32.
In this study, polycarbonates are functionalized with guanidinium groups to provide biodegradable broad-spectrum polyguanidiniums. From octanol–water partition, bacterial membrane integrity, SEM and TEM analyses, at the effective concentration, the antibacterial function of the polymer is mainly based on membrane translocation followed by precipitation of bacterial cytoplasmic contents (e.g., proteins and genes). These polymers are demonstrated to be effective in treating MDR A. baumannii, E. coli, K. pneumoniae, P. aeruginosa, and MRSA infections in vivo with negligible toxicity while mitigating drug resistance.
To synthesize guanidinium-functionalized polycarbonates, guanidinium-functionalized alcohol precursors were first made by a facile, efficient, and modular route as depicted in Fig. 1a. Using 1,3-bis(tert-butoxycarbonyl)-2-methyl-2-thiopseudourea as the guanylating agent, the respective primary amine group belonging to a structurally diverse range (e.g., homologous straight chain alkyl, cyclohexyl, aromatic, etc.) of amino alcohols reacted readily under ambient conditions in desirable quantitative yields accompanied by the concomitant loss of a primary thiol by-product (i.e., methanethiol, MeSH) to afford the consequent Boc-protected guanylated alcohols (Supplementary Figure 1). In addition to its low cost, rapid reaction rates and high yields as compared to other conventional guanylating agents, e.g., pyrazoles33, the use of methylisothioureas in guanidine synthesis is advantageous in several ways34. Its use can be extended to the less reactive aromatic amines, such as p-aminophenol with good yields; there is also ease of isolation and purification upon reaction completion by virtue of the gaseous nature of the thiol by-product. The desired guanidinium-functionalized monomers were efficiently accessed through conversion of the cyclic carbonate carboxylic acid (i.e., MTC-OH) to an acid chloride intermediate in situ, followed by an esterification with the aforementioned guanylated alcohols23, 24 (Supplementary Figure 1). This illustrates the amenability and expeditiousness of our synthetic strategy.
Polymerization was subsequently carried out by metal-free organocatalytic ROP of Boc-protected guanidine-functionalized carbonate monomers containing the various requisite hydrophobic spacer moieties (Fig. 1b). In situ analysis by gel permeation chromatography (GPC) demonstrated more than 90% consumption of the respective monomer (MTC-OX-BocGua) within a remarkably short reaction time of 30 min at room temperature. By referencing the integrated intensities of relevant resonances attributed from the terminal initiator with respect to the polymer side chains, 1H NMR characterization of the resulting polymers elucidated that the average DP (degree of polymerization) obtained was in good agreement with that predicted from initial monomer/initiator feed ratio (Supplementary Figure 1 and Fig. 2). Furthermore, all polymers obtained exhibited narrow molecular weight distribution with a polydispersity index (Ɖ) ranging between 1.1 and 1.2, as ascertained by GPC prior to deprotection of the polymers’ Boc groups (Fig. 1c). Upon purification of the Boc-protected polycarbonates, a facile removal of the Boc groups with trifluoroacetic acid afforded the desired water-soluble guanidinium-functionalized polymers (i.e., P(MTC-OX-Gua)). Taken together, these results thus exemplify the expedient and highly controlled ability of the organocatalytic ROP for acquiring functional and well-defined polycarbonates with predictable molecular weights and narrow molecular weight distribution. Such properties are deemed particularly pivotal for an unambiguous and systematic elucidation of the role that the various structural determinants (molecular length and hydrophobic spacer) have on biological activity to optimize activity and selectivity. The polymers are labeled according to the structure of the hydrophobic spacer and the DP predicted from initial monomer/initiator feed ratio. As an example, pEt_20 denotes a guanidinium-functionalized polycarbonate containing 20 repeating units with an ethyl group as the hydrophobic spacer.
Antimicrobial and hemolytic activities
The influence of the polymers’ molecular length was first studied on antimicrobial and hemolytic activities by varying DP from 5 to 40. The polymer pEt_20 exhibited the strongest antimicrobial activity against a broad panel of pathogenic microbes with minimum inhibitory concentrations (MICs, the lowest polymer concentrations that completely inhibited microbial growth) in the range of 7.8–15.6 µg/mL and the lowest geometric mean (Gm) value of MICs (Supplementary Table 1). The MICs of pEt_20 were much lower than the previous antimicrobial polycarbonates bearing quaternary ammonium groups12, 19,20,21,22. Remarkably, pEt_20 possessed a pronounced lack of toxicity toward rat red blood cells (HC50, polymer concentration that induces 50% hemolysis: >8000 μg/mL) (Supplementary Table 1). This is the highest HC50 value that has ever been reported for biodegradable synthetic antimicrobial polymers12, 19,20,21,22. Serum content was less than 2% in the hemolysis assay. The MIC values of pEt_20 (and pEt_10) against the panel of bacteria were determined to be the same in the presence of 2% serum. Lack of interaction with proteins was possibly due to diffused charge in the guanidinium group. Its selectivity (HC50/Gm and HC5/Gm, >584) is significantly higher than those of many host defense peptides, e.g., cecropin A (HC5/Gm = 0.034)35, porcine protegrin-1 (HC50/Gm = 2.3)35, magainins (HC50/MIC = 10)36 and defensins (rMicasin: HC5/Gm = 4)37, synthetic peptides38, 39, guanidinium-functionalized non-degradable polynorbornenes (HC50/Gm, 75)31, and polymethacrylates (HC5/Gm, <2)32, and recently reported star-shaped peptide PAMAM-[poly(lysine-co-valine)]32 (HC5/Gm, 23)40. Moreover, pEt_20 and pEt_10 had significantly lower cytotoxicity against human embryonic kidney (HEK293T) cells after 18-h incubation than polymyxin B (Supplementary Figure 2) and PAMAM-[poly(lysine-co-valine)]32 (shorter incubation time: 90 min)40 (IC50, inhibitory concentration of compound that leads to 50% cell viability: >500, >500, 165, and 128 μg/mL, respectively).
Having established the optimal DP to be 20 for the highest potency and selectivity, the influence of the hydrophobic spacer was further investigated for a series of polymers bearing different spacer group structures encompassing of aliphatic and aromatic types (Fig. 2). The alkyl series (pEt_20, pPr_20, and pBut_20) had greater antimicrobial potency and higher HC50 than the aromatic ones (pPh_20 and pBn_20). Increasing the alkyl length from ethyl to pentyl or cyclohexyl led to a dramatic increase in hemolysis. This is largely attributed to the corresponding increase in polymer’s hydrophobicity that might enhance its hydrophobic interaction with cell membrane, facilitating membrane disruption. Although oligoarginine peptide R20 has the same number of guanidinium groups and the same hydrophobic spacer as pPr_20, its antimicrobial activity was significantly lower (Fig. 2). R10 was even less potent. This stark difference might be due to enhanced membrane translocation of polymer and/or stronger interaction between the polymer and cytoplasmic proteins/genes stemming from the more hydrophobic polycarbonate backbone as compared to peptide backbone. As pEt_10 and pEt_20 exhibited strong antimicrobial activity and high selectivity, they were chosen for further studies.
Antimicrobial activity against clinically isolated MDR bacteria
To evaluate the potential of the polymers as effective antimicrobials for future clinical applications, pEt_10 and pEt_20 were assayed against four of the most opportunistic and MDR bacteria in both developing and developed healthcare systems, Gram-negative A. baumannii, K. pneumoniae, and E. coli as well as Gram-positive MRSA (Supplementary Table 2). These bacteria are part of “ESKAPE” pathogens1, and are resistant to multiple antibiotics including polymyxin B (Supplementary Table 2). The polymers demonstrated efficacious antimicrobial activity and favorable selectivity toward the clinically isolated MDR bacteria (Fig. 3a), in concordance with efficacy seen against commercially available ATCC strains (Fig. 2). In sharp contrast, the oligoarginine peptides R10 and R20 were not effective against the bacteria even at 512 µg/mL. The presence of 10% serum did not alter the MIC values of both pEt_10 and pEt_20 against these MDR clinical isolates. A similar phenomenon was also observed in guanidinium-functionalized non-degradable polymethacrylates32. In the presence of 40% serum, MIC increased by 1–7 times (1 time for MRSA and A. baumannii, 1–3 times for K. pneumoniae, and 7 times for E. coli). The increases in MIC for MRSA and ATCC S. aureus (1 time) were lower than those for small molecular synthetic foldamers against ATCC S. aureus (31–63 times increase in MIC in the presence of 40% serum), which showed high in vivo efficacy in a S. aureus-caused mouse thigh infection model41, indicating that pEt_10 and pEt_20 had less protein interaction than the foldamers.
In vitro killing efficiency and kinetics
The antimicrobial activity of the polymers was further investigated by colony forming unit (CFU) assay against a representative strain of clinically isolated MDR bacteria, A. baumannii 10073, E. coli 56809, K. pneumoniae 8637, and MRSA 25312, in comparison with antibiotics imipenem (control for the Gram-negative bacteria) and vancomycin (control for MRSA). The A. baumannii and K. pneumoniae strains were resistant to imipenem, whereas the E. coli and MRSA strains were susceptible to imipenem and vancomycin, respectively (Supplementary Table 3). Like imipenem, at 1× MIC, 2× MIC, or 4× MIC, pEt_10 and pEt_20 demonstrated ∼100% killing efficiency against A. baumannii (Supplementary Figure 3). In the case of E. coli and K. pneumoniae, they achieved more than 99.9% efficiency at 2 × MIC and 1 × MIC, respectively (Supplementary Figure 3). Killing efficiency of vancomycin against MRSA was less than 99% (2-log reduction in bacterial counts) even at 4 × MIC, while pEt_10 and pEt_20 eliminated more than 99.9% MRSA (3-log reduction in bacterial counts) at 2 × MIC and 1 × MIC, respectively. These results clearly demonstrated bactericidal activity of the polymers against both Gram-positive and Gram-negative MDR bacteria. Although imipenem and vancomycin were shown to be bactericidal (more than 3-log reduction in bacterial counts) at 1 × MIC and 2-log reduction in bacterial counts at 2 × MIC, respectively, their efficacies were only observed after 18 h of treatment (Supplementary Figure 3). They were unable to eradicate 99.9% bacteria after 1 h (A. baumannii), 2 h (K. pneumoniae), or 3 h (MRSA, E. coli) of treatment even at 4 × MIC (Fig. 3, Supplementary Figure 4, and Supplementary Figure 5). In sharp contrast, pEt_10 and pEt_20 eliminated these MDR bacteria effectively even at shorter treatment durations (Fig. 3, Supplementary Figure 4, and Supplementary Figure 5). For example, pEt_20 eradicated ~99.9% A. baumannii at 1 × MIC within 10 min (Fig. 3b). An increase in polymer concentration resulted in faster elimination of bacteria. In addition, pEt_20 with a longer polymer chain eradicated bacteria more efficiently especially at higher concentrations. Such promising in vitro results with these polymers certainly warrant further in vivo studies for the treatment of MDR infections.
To shed light on the mode of action of the guanidinium-functionalized polycarbonate, a number of techniques were employed to understand polymer-bacterial membrane interaction and cause of efficient cell death with high selectivity. Octanol–water partition study was first performed using the fluorescent dye dansyl-labeled pEt_20 and corresponding polycarbonate containing quaternary ammonium (Supplementary Figure 6). Both polymers partitioned almost exclusively into the aqueous phosphate-buffered saline (PBS) layer as a consequence of the charged nature of the polymers. However, upon the addition of a model surrogate for a membrane-bound fatty acid salt (sodium laurate) into the octanol layer, pEt_20 was seen to partition significantly into the octanol layer with just 0.5 equivalents of sodium laurate. As the concentration of the fatty acid salt increased, pEt_20 was virtually partitioned only within the octanol layer. In contrast, the quaternary ammonium polymer stayed in the aqueous layer even at 2.0 equivalents of the fatty acid salt added. This finding showed that pEt_20 effectively translocated into the membrane-mimic lipophilic layer. Next, the integrity of bacterial membrane was studied after treatment with pEt_20 or the membrane-lytic polymyxin B by testing leakage of cytoplasmic materials (e.g., proteins and genes) with absorbance at 260 nm. No significant leakage of cytoplasmic materials was detected from A. baumannii cells after 2-h pEt_20 treatment at MBC (minimum bactericidal concentration that leads to 99.9% bacteria killing in 2 h) and below although leakage was seen at a higher concentration (2 × MBC) (Fig. 4). This indicated that pEt_20 killed the bacteria without lysing membrane at the effective dose. In contrast, significant membrane leakage was observed with polymyxin B treatment even at 0.5 × MBC.
The morphological changes in A. baumannii were further observed under scanning electron microscopy (SEM) before and after polymer treatment. No membrane lysis was seen in the majority of the cells after 2-h treatment with either pEt_10 or pEt_20 at 4 × MIC, and only a few cells had distorted membrane (Fig. 4a, b). Although prolonging the treatment time from 2 to 6 h led to more cells with disrupted membrane, a larger population of cells had an intact membrane (Supplementary Figure 7). Similar phenomena were also observed under transmission electron microscopy (TEM) after 2-h polymer treatment at 4 × MIC and 16 × MIC (Fig. 4c, d and Supplementary Figure 7). Particularly, the TEM micrographs of polymer-treated bacteria revealed precipitation of the bacterial cytoplasm with an intact membrane in a larger population of the cells at both concentrations (see arrows in Fig. 4c, d and Supplementary Figure 7), which was likely caused by polymer interactions with cytoplasmic materials (e.g., proteins and genes) upon membrane translocation. Similarly, non-biodegradable broad-spectrum antimicrobial polyhexamethylene biguanide was recently reported to enter bacterial cells, bind DNA and condense chromosome, leading to cell death42. This unique antimicrobial mechanism provides the polymers with higher potency and greater selectivity than quaternary ammonium-containing polycarbonates that function based on the membrane-disruption mechanism (Fig. 2)12, 19,20,21,22. Moreover, another key consideration with this novel mechanism over membrane lysis is the minimization of possibility of septicemia from the rapid release of cytosolic content. This distinctive mechanism warrants further investigation and will be the topic of future studies as it distinguishes this new class of polymers from antimicrobial peptides that have been widely studied.
Prevention of resistance development
To investigate if repeated use of the polymers with the unique antimicrobial mechanism develops resistance, A. baumannii 10073 cells were treated with pEt_10 or pEt_20 for 30 passages at sub-MIC concentrations and MIC was determined in each passage of the bacteria. As shown in Fig. 4e, repeated use of these polymers did not cause resistance development in A. baumannii even after 30 passages, while multiple treatments with imipenem at sublethal doses developed resistance after 8 passages as a result of reduced antimicrobial effect. A similar phenomenon was also observed previously for the antibiotics gentamicin in E. coli43 and vancomycin in MRSA25, and for gentamicin in K. pneumoniae as shown in Supplementary Figure 8.
RNA-seq analysis of bacterial resistance
In order to systematically understand bacterial response to different xenobiotic stress, i.e., antibiotic or polymer, bacterial RNA-seq was performed on MDR A. baumannii 10073 cultures after 30 passages. An MDR strain AB030 was used to recapitulate known imipenem response genes as a proof of concept and to compare it to pEt_20 treatment. A total of 506 genes were differentially regulated [Log2-fold change 1; 5% false discovery rate (FDR)] upon imipenem treatment relative to the untreated control (Supplementary Data 1 and Fig. 5). KEGG pathway analyses of the significant genes implicated pathways that are involved in beta-lactam, vancomycin, and cationic antimicrobial peptide resistances. Importantly, the predominant efflux pump-mediated carbapenem resistance genes from the resistance nodulation (RND) family, i.e., adeA and adeB, were both upregulated upon imipenem treatment44, 45. In addition, ftsI (penicillin binding protein 3) was also shown to be differentially regulated, possibly conferring carbapenem resistance46. We were also able to recapitulate some of the known categories implicated in imipenem resistance including quorum sensing and biofilm formation44, 47. However, in the case of pEt_20, there were fewer differentially regulated genes that were significant (n = 206; Supplementary Data 2, Fig. 5, Log2-fold change 1; 5% FDR) and no apparent transcriptional response in any known resistance pathways (KEGG database) were observed relative to pEt_20 treatment.
A systematic comparison of imipenem and pEt_20 response genes resulted in 47 common genes (Fig. 5a). Interestingly, a majority (~78%) of these common genes show opposite patterns for pEt_20 and imipenem treatments (Fig. 5b). Overall, the above data suggest that imipenem and polymer response genes have mutually exclusive functions, while the polymer has no observed propensity for resistance development. This merits in effective treatment possibilities for polymer-based antimicrobial agents to combat resistance.
Evaluation of in vivo toxicity and immunogenicity
To evaluate these polymers for in vivo application to treat drug-resistant infections, LD50/LD5 (single lethal dose resulting in 50% and 5% mice mortality, respectively) values of pEt_10 and pEt_20, were first determined. No significant differences in toxicity were observed with both polymers as evidenced by similar LD50 and LD5 values (Fig. 6a). In addition, immunogenicity of the polymers was evaluated in mouse peripheral blood mononuclear cells (PBMCs) by testing secretion of the pro-inflammatory cytokines IFN-γ and TNF-α by PBMCs after polymer treatment. The secretion of the cytokines may result in undesirable non-specific immunogenic response in vivo. The treatment with the bacterial lipopolysaccharides (LPS) stimulated a high level of IFN-γ and TNF-α secretion, while pEt_10 or pEt_20 treatment showed negligible immunogenic response as compared to the negative control without any treatment (Supplementary Figure 9).
In vivo biodistribution and pharmacokinetics
From the biodistribution study of a NIR dye (AF750)-labeled pEt_20 (Supplementary Figure 10), the polymer was found in mouse liver, spleen, lungs, and kidneys after i.p. or i.v. injection, indicating that the polymer penetrated tissues and got into the blood stream even after i.p. injection. The pharmacokinetics study of the dye-labeled polymer demonstrated its mean plasma elimination half-life of ∼17 min (Supplementary Tables 4 and 5), which is comparable to (if not longer than) that of the clinically used antibiotics meropenem (6 min), imipenem (12 min), and doripenem (12 min)48. Taken together with rapid killing kinetics (Fig. 3b, c, Supplementary Figure 4, and Supplementary Figure 5), the polymers have potential utility in vivo.
In vivo antimicrobial activity
The in vivo treatment efficacy of the polymers was then investigated in A. baumannii 10073-, E. coli 56809-, K. pneumoniae 8637-, and MRSA 25312-induced peritonitis mouse models. The minimum lethal doses of A. baumannii, E. coli, K. pneumoniae, and MRSA sufficient to cause 100% mortality within 48 h through intraperitoneal (i.p.) injection were pre-determined: 2.7 × 108, 8.8 × 106, 2.6 × 108, and 5.2 × 108 CFU/mL (0.5 mL), respectively. Mice were inoculated with the bacteria at their respective lethal doses by i.p. injection, and subsequently treated with two i.p. injections of pEt_10, pEt_20, imipenem (control for the Gram-negative bacteria), or vancomycin (control for MRSA) at 1 and 6 h or 3 and 8 h post infection. Both polymers demonstrated high treatment efficacy across all four infection models with low ED50/ED95 (effective dose resulting in 50 and 95% survival of infected mice) values, which were well below their LD5 values especially in A. baumannii infection (Fig. 6a), showing a wide therapeutic window (e.g., therapeutic index—ED50/LD50: 1473 for A. baumannii infection). Delay in the treatment by 2 h slightly increased ED50/ED95 values. In imipenem-susceptible E. coli and vancomycin-susceptible MRSA infections, the polymers had similar ED50/ED95 as compared to the antibiotics. However, in imipenem-resistant A. baumannii and K. pneumoniae infections, the polymers displayed higher efficacy with lower ED50/ED95 than imipenem. In addition, pEt_20 achieved better treatment efficacy than pEt_10 (lower ED50/ED95 for pEt_20). This may be in part attributable to pEt_20’s superior bactericidal kinetics (Fig. 3b, c, Supplementary Figure 4, and Supplementary Figure 5).
In vivo elimination efficiency of bacteria was subsequently assessed in A. baumannii 10073- and E. coli 56809-induced peritonitis mouse models. The polymers and imipenem effectively removed bacteria from the blood, peritoneal fluid, and organs with >99.0–99.9% efficiency at their respective ED95 doses as compared to the control without any treatment at 24 h post infection (Fig. 6b, c). Particularly, in the A. baumannii infection, pEt_20 exhibited higher efficacy than imipenem (Fig. 6b) primarily because pEt_20 had faster killing kinetics (Fig. 3b). Effective removal of bacteria is key to ensuring survival against bacterial sepsis. This limits the production and consequent circulation of deleterious bacterial endo- and exo-toxins otherwise responsible for septic shock and multi-organ dysfunction syndrome.
The treatment efficacy of polymer was further evaluated in a cecal ligation and puncture (CLP)-induced polymicrobial peritonitis mouse model, the most commonly used sepsis model with comparable features of peritonitis in humans, where a systemic infection was induced by release of fecel material into peritoneal cavity49. As there are both Gram-positive and Gram-negative bacteria present in fecal material, the broad-spectrum antibiotic gentamicin was used as control. With a single day treatment, pEt_20 achieved comparable in vivo efficacy as compared to gentamicin in terms of mouse survival (Fig. 7a) and bacterial removal efficiency (Fig. 7b, c).
To demonstrate in vivo efficacy in treating a distal infection, the polymer was used to treat a clinical strain of P. aeruginosa (PA14)-caused lung infection in a mouse model by intravenous injection (MIC of pEt_10 against PA14: 25 µg/mL; 8 mg/kg of mouse body weight per injection, 3 i.v. injections at 1, 6, and 25 h post infection). A single day treatment with pEt_10 saved five out of six infected mice, while imipenem treatment saved four infected mice (Fig. 7d). In addition, the polymer was more effective in removing the bacteria from the blood and the lung tissues (Fig. 7e, f).
More importantly, mice treated with the polymers at 8 mg/kg mouse body weight, 0.2 mL per injection, twice daily for 3 consecutive days (48 mg/kg in total), which is close to the highest dose used in this study (50 mg/kg), did not induce nephro- or hepato-toxicity, nor any electrolyte disturbances (Supplementary Table 6). There was also negligible hemolysis as evidenced by normal blood potassium levels. This may be explained by complete degradation of the polymer after 3 days (Supplementary Figure 11), and negligible toxicity of the degradation products (Supplementary Figure 12). These results suggest negligible polymer toxicity especially when administered at their effective dosages.
In this study, we have demonstrated high in vivo efficacy of the guanidinium-functionalized polycarbonates with broad-spectrum activity including MDR A. baumannii-, K. pneumonia-, E. coli-, MRSA-, P. aeruginosa-, and CLP-induced polymicrobial systemic infections, and P. aeruginosa lung infection. The multiple treatments of bacteria with the polymers do not develop drug resistance, owing to a distinctive new mechanism, as evidenced in an in vitro evolution model and genetic sequencing. Polymer treatment at the effective doses does not cause acute systemic toxicity in vivo. It is envisaged that these polymers bear great potential as antimicrobial agents in the prevention and treatment of multidrug-resistant systemic infections.
N-(3,5-trifluoromethyl) phenyl-N′-cyclohexylthiourea (TU) was prepared according to our previous protocol23, 24. TU was dissolved in dry tetrahydrofuran (THF), stirred with CaH2, filtered, and freed of solvent in vacuo. Prior to use, 1,8-diazabicyclo [5,4,0]undec-7-ene (DBU) was stirred over CaH2 and vacuum distilled before being transferred to a glove box. All other chemical reagents were purchased from Sigma-Aldrich and used as received unless specified. Ultra pure (HPLC grade) water was obtained from J.T. Baker (USA). Phosphate-buffered saline (PBS) at 10× concentration was purchased from first BASE (Singapore) and diluted to the intended concentrations before use. Cation-adjusted Mueller–Hinton broth (MHB) powder was bought from BD Diagnostics (Singapore) and used to prepare the microbial broths according to the manufacturer’s instructions. Staphylococcus aureus (ATCC No. 6538), Escherichia coli (ATCC No. 25922), Pseudomonas aeruginosa (ATCC No. 9027), and yeast Candida albicans (ATCC No. 10231) were obtained from ATCC (USA) and reconstituted according to the suggested protocols. Clinically isolated MDR bacteria that were extracted from blood or phlegm samples of the patients hospitalized in The First Affiliated Hospital of Medical College, Zhejiang University (Hangzhou, China) were kindly provided by Dr. Kaijin Xu. All isolates were identified by routine laboratory methods and stored in 20% (v/v) glycerol at −80 °C prior to use. Drug susceptibility of the bacteria was evaluated according to Clinical Laboratory Standards Institute (CLSI) (Supplementary Table 2).
Synthesis of Boc-protected guanylated alcohol precursors
For the guanylation of amino alcohols, a detailed protocol for the synthesis using ethanolamine as the starting reagent is described as a representative example (Figure 1a). To a solution mixture of ethanolamine (1.37 mL, 22.8 mmol, 2.0 equiv) and N,N-diisopropylethylamine (DIPEA) (6.0 mL, 34.4 mmol, 3.0 equiv) was added 1,3-bis(tert-butoxycarbonyl)-2-methyl-2-thiopseudourea (3.3 g, 11.6 mmol, 1.0 equiv) in 20 mL of dry CH2Cl2, and the mixture was left to stir overnight at room temperature. Upon reaction completion, a constant stream of nitrogen gas was bubbled through the reaction mixture for ~1 h so as to aid in purging of the gaseous by-product, MeSH. After the removal of residual solvent in vacuo, the crude product was purified by flash column chromatography using silica gel and a hexane-ethyl acetate solvent system as the eluent (gradient elution up to 50% vol. ethyl acetate) to yield the Boc-protected guanylated alcohol (HO-Et-BocGua) as a white solid (3.2 g, 10.4 mmol, 90% yield). 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.45 (s, 1H, NH), 8.72 (s, 1H, NH), 3.77 (dd, J = 5.3, 3.9 Hz, 2H, –CH2–), 3.57 (dd, J = 9.2, 5.5 Hz, 2H, –CH2–), 1.49 (d, J = 8.2 Hz, 18H, Boc –CH3).
HO-Pr-BocGua: Yield: 87%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.44 (s, 1H, NH), 8.47 (s, 1H, NH), 3.57 (dt, J = 12.1, 5.8 Hz, 4H, –CH2–), 1.72–1.66 (m, 2H, –CH2–), 1.48 (d, J = 9.9 Hz, 18H, Boc –CH3).
HO-But-BocGua: Yield: 90%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.48 (s, 1H, NH), 8.39 (s, 1H, NH), 3.69 (t, J = 6.1 Hz, 2H, –CH2–), 3.49–3.41 (m, 2H, –CH2–), 1.71–1.59 (m, 4H, –CH2–), 1.49 (d, J = 2.3 Hz, 18H, Boc –CH3).
HO-Pen-BocGua: Yield: 89%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.49 (s, 1H, NH), 8.31 (s, 1H, NH), 3.64 (t, J = 6.5 Hz, 2H, –CH2–), 3.42 (td, J = 7.2, 5.4 Hz, 2H, –CH2–), 1.64–1.56 (m, 4H, –CH2–), 1.49 (d, J = 3.6 Hz, 18H, Boc –CH3), 1.43 (ddd, J = 12.4, 5.7, 3.0 Hz, 2H, –CH2–).
HO-Cy-BocGua: Yield: 82%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.53 (s, 1H, NH), 8.24 (s, 1H, NH), 4.08–3.95 (m, 1H, –CH), 3.70–3.59 (m, 1H, –CH), 2.12–2.04 (m, 2H, –CH2–), 2.00–1.91 (m, 2H, –CH2–), 1.49 (d, J = 7.7 Hz, 18H, Boc –CH3), 1.45–1.38 (m, 2H, –CH2–), 1.32–1.22 (m, 2H, –CH2–).
HO-Ph-BocGua: Yield: 72%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.62 (s, 1H, NH), 9.95 (s, 1H, NH), 7.08–7.02 (m, 2H, Ph –CH), 6.63–6.53 (m, 2H, Ph –CH), 1.49 (d, J = 34.8 Hz, 18H, Boc –CH3).
HO-Bn-BocGua: Yield: 85%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.53 (s, 1H, NH), 8.53 (s, 1H, NH), 7.19–7.09 (m, 2H, Ph –CH), 6.84–6.73 (m, 2H, Ph –CH), 4.52 (d, J = 5.1 Hz, 2H, –CH2–), 1.49 (d, J = 12.4 Hz, 18H, Boc –CH3).
Synthesis of MTC-OX-BocGua monomers
MTC-OX-BocGua was synthesized with reference to the protocol reported in the previous work33 (Figure 1a). Briefly, in a dry three-neck round bottom flask equipped with a stir bar, MTC-OH (3.08 g, 19.3 mmol) was dissolved in dry THF (50 mL) with a 3–4 drops of dimethylformamide (DMF). A solution of oxalyl chloride (2.45 mL, 28.5 mmol) in THF (50 mL) was subsequently added from a dropping funnel. Under an inert atmosphere, the solution was stirred for 1 h, after which volatiles were removed under vacuum, yielding an off-white solid (i.e., 5-chlorocarboxy-5-methyl-1,3-dioxan-2-one intermediate). The solid was heated to 60 °C for a brief 2–3 min to remove any residual solvent, and then re-dissolved in dry CH2Cl2 (50 mL) and cooled down to 0 °C via an ice bath. A mixture of the relevant Boc-protected guanylated alcohol (e.g., HO-Et-BocGua, 5.4 g, 17.8 mmol) and pyridine (1.55 mL, 19.3 mmol) dissolved in dry CH2Cl2 (50 mL) was then added dropwise over a duration of 30 min, and allowed to stir at 0 °C for an additional 30 min before leaving it at ambient temperature for further stirring overnight. After removal of solvent, the crude product was subjected to purification by flash column chromatography using silica gel and a hexane-ethyl acetate solvent system as the eluent (gradient elution up to 80% vol. ethyl acetate) to yield MTC-OEt-BocGua as a white solid (78% yield). 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.48 (s, 1H, NH), 8.64 (s, 1H, NH), 4.71 (d, J = 10.9 Hz, 2H, CH a Hb), 4.33 (t, J = 5.2 Hz, 2H, –OCH2–), 4.22 (d, J = 10.9 Hz, 2H, CHaH b ), 3.77 (d, J = 5.1 Hz, 2H, –CH2N–), 1.49 (d, J = 1.8 Hz, 18H, Boc –CH3), 1.39 (d, J = 3.4 Hz, 3H, –CH3).
MTC-OPr-BocGua: Yield: 75%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.50 (s, 1H, NH), 8.40 (s, 1H, NH), 4.72 (d, J = 10.9 Hz, 2H, CH a Hb), 4.26 (t, J = 6.1 Hz, 2H, –OCH2–), 4.21 (d, J = 10.9 Hz, 2H, CHaH b ), 3.53 (dd, J = 12.5, 6.7 Hz, 2H, –CH2N–), 1.99–1.91 (m, 2H, –CH2–), 1.49 (d, J = 1.7 Hz, 18H, Boc –CH3), 1.37 (s, 3H, –CH3).
MTC-OBut-BocGua: Yield: 75%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.44 (s, 1H, NH), 8.30 (t, J = 4.6 Hz, 1H, NH), 4.64 (d, J = 10.8 Hz, 2H, CH a Hb), 4.20–4.14 (m, 4H, CHaH b and –OCH2–), 3.40 (dd, J = 12.4, 6.9 Hz, 2H, –CH2N–), 1.68 (dd, J = 5.3, 3.0 Hz, 2H, –CH2–), 1.63–1.57 (m, 2H, –CH2–), 1.44 (s, 18H, Boc –CH3), 1.28 (s, 3H, –CH3).
MTC-OPen-BocGua: Yield: 79%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.50 (s, 1H, NH), 8.33 (s, 1H, NH), 4.68 (d, J = 10.8 Hz, 2H, CH a Hb), 4.20 (m, J = 11.4, 4.5 Hz, 4H, CHaH b and –OCH2–), 3.43 (dd, J = 12.5, 6.8 Hz, 2H, –CH2N–), 1.70 (dd, J = 14.8, 7.0 Hz, 2H, –CH2–), 1.63–1.58 (m, 2H, –CH2–), 1.50 (d, J = 3.6 Hz, 18H, Boc –CH3), 1.46–1.38 (m, 2H, –CH2–), 1.34 (s, 3H, –CH3).
MTC-OCy-BocGua: Yield: 60%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.50 (s, 1H, NH), 8.31 (s, 1H, NH), 4.88–4.77 (m, 1H, –CH), 4.67 (d, J = 10.8 Hz, 2H, CH a Hb), 4.18 (d, J = 10.8 Hz, 2H, CHaH b ), 4.14–4.01 (m, 1H, –CH), 2.09 (dd, J = 13.2, 3.3 Hz, 2H, –CH2–), 2.01–1.94 (m, 2H, –CH2–), 1.62–1.56 (m, 2H, –CH2–), 1.49 (d, J = 6.0 Hz, 18H, Boc –CH3), 1.42–1.32 (m, 2H, –CH2–), 1.31 (s, 3H, –CH3).
MTC-OPh-BocGua: Yield: 75%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.62 (s, 1H, NH), 10.40 (s, 1H, NH), 7.65 (d, J = 9.0 Hz, 2H, Ph –CH), 7.05 (d, J = 9.0 Hz, 2H, Ph –CH), 4.84 (d, J = 11.0 Hz, 2H, CH a Hb), 4.31 (d, J = 10.9 Hz, 2H, CHaH b ), 1.52 (d, J = 14.3 Hz, 21H, Boc –CH3 and –CH3).
MTC-OBn-BocGua: Yield: 78%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.53 (s, 1H, NH), 8.67 (s, 1H, NH), 7.35 (dd, J = 6.6, 4.7 Hz, 2H, Ph –CH), 7.10–7.04 (m, 2H, Ph –CH), 4.84 (d, J = 11.0 Hz, 2H, CH a Hb), 4.66 (d, J = 5.2 Hz, 2H, –CH2–), 4.32 (d, J = 10.9 Hz, 2H, CHaH b ), 1.53–1.47 (m, 21H, Boc –CH3 and –CH3).
Synthesis of P(MTC-OX-BocGua) polymers
The detailed procedures for the ring-opening polymerization (ROP) of MTC-OEt-BocGua with 4-methyl benzyl alcohol as initiator are given as a representative example (Figure 1b). Using a glove box, the 4-methyl benzyl alcohol (6.1 mg, 0.05 mmol) was added to a reaction vial containing TU (18.5 mg, 0.05 mmol) and DBU (7.47 μL, 0.05 mmol) dissolved in dry DCM (1 mL) and left to stir for about 10 min. The mixture was subsequently charged with MTC-OEt-BocGua (445 mg, 1.0 mmol) and left to stir at room temperature for an additional 30 min. The reaction was stopped by quenching the catalyst using an excess of benzoic acid (10 mg, 0.08 mmol). The crude polymer was isolated and purified via preparative size-exclusion chromatography using THF as the eluent. Upon removal of the solvent in vacuo, a transparent white solid was obtained as the product, P(MTC-OEt-BocGua) _ 20 (79% yield). 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.47 (s, 22H, NH), 8.61 (bs, 22H, NH), 4.41–4.20 (m, 129H, CH a Hb, CHaH b and –OCH2–), 3.79–3.65 (m, –CH2N–, overlapped with residual THF peak), 2.34 (s, 3H, initiator –CH3), 1.47 (d, J = 14.3, 427H, Boc –CH3), 1.24 (bs, 69H, –CH3).
P(MTC-OPr-BocGua)_20: Yield: 74%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.49 (s, 18H, NH), 8.43 (bs, 18H, NH), 5.10 (s, 2H, initiator –CH2–), 4.36–4.25 (m, 69H, CH a Hb and CHaH b ), 4.23–4.17 (m, 39H, –OCH2–), 3.56–3.46 (m, 37H, –CH2N–), 2.34 (s, 3H, initiator –CH3), 1.93 (m, 38H, –CH2–), 1.46 (d, J = 23.0 Hz, 386H, Boc –CH3), 1.24 (bs, 69H, -CH3).
P(MTC-OBut-BocGua)_20: Yield: 72%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.50 (s, 18H, NH), 8.36 (bs, 18H, NH), 5.09 (s, 2H, initiator –CH2–), 4.29 (m, 66H, CH a Hb and CHaH b ), 4.14 (m, 35H, –OCH2–), 3.45 (m, 36H, –CH2N–), 2.34 (s, 3H, initiator –CH3), 1.72–1.63 (m, 71H, –CH2–), 1.53–1.41 (d, 363H, Boc –CH3), 1.28–1.18 (bs, 57H, –CH3).
P(MTC-OPen-BocGua)_20: Yield: 70%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.53 (s, 19H. NH), 8.41 (bs, 19H, NH), 5.12 (s, 2H, initiator –CH2–), 4.44–4.23 (m, 80H, CH a Hb and CHaH b ), 4.14 (m, 43H, –CH2–), 3.46 (m, 42H, –CH2–), 2.36 (s, 3H, initiator –CH3), 1.66 (m, 101H, –CH2–), 1.49 (d, J = 22.2, 4.7 Hz, 410H, Boc –CH3), 1.48–1.34 (m, 70H, –CH2–), 1.26 (bs, 60H, –CH3).
P(MTC-OCy-BocGua)_20: Yield: 71%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.53 (s, 19H, NH), 8.35 (bs, 18H, NH), 5.12 (s, 2H, initiator –CH2–), 4.80 (m, 19H, –CH), 4.37–4.22 (m, 69H, CH a Hb and CHaH b ), 4.10 (s, 19H, –CH2–), 2.37 (s, 3H, initiator –CH3), 2.10 (m, 41H, –CH2–), 1.96 (m, 40H, –CH2–), 1.64 (m, 40H, –CH2–), 1.49 (d, J = 13.7 Hz, 416H, Boc -CH3), 1.38 (m, 43H, –CH2–), 1.23 (bs, 64H, –CH3).
P(MTC-OPh-BocGua)_20: Yield: 70%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.64 (s, 16H, NH), 10.34 (bs, 16H, NH), 7.54 (m, 35H, Ph –CH), 7.04–6.97 (m, 36H, Ph –CH), 5.12 (s, 2H, initiator –CH2–), 4.67–4.16 (m, 88H, CH a Hb and CHaH b ), 2.34 (s, 3H, initiator –CH3), 1.56–1.42 (m, 383H, Boc –CH3 and –CH3).
P(MTC-OBn-BocGua)_20: Yield: 70%; 1H-NMR (400 MHz, CDCl3, 22 °C): δ 11.54 (s, 17H, NH), 8.61 (bs, 17H, NH), 7.30 (m, 35H, Ph –CH), 7.11–6.98 (m, 47H, Ph –CH), 5.12 (s, 2H, initiator –CH2–), 4.61 (m, 39H, –CH2–), 4.48 (m, 76H, CH a Hb and CHaH b ), 2.35 (s, 3H, initiator –CH3), 1.55–1.41 (m, 464H, Boc –CH3 and –CH3).
Synthesis of deprotected P(MTC-OX-BocGua) polymers (P(MTC-OX-Gua))
For the post-polymerization removal of Boc groups, an acid-mediated deprotection strategy was adopted (Figure 1b). Briefly, P(MTC-OEt-BocGua)_20 (150 mg) was dissolved in CH2Cl2 (9 mL) and trifluoroacetic acid (1 mL). The reaction mixture was sealed and stirred at room temperature for 14–18 h. After the removal of solvent in vacuo, slightly yellow waxy solid was obtained as the deprotected guaninidium-functionalized polymer in quantitative yields. The polymer was subsequently dissolved in water and lyophilized to yield a white transparent solid, pEt_20. Complete deprotection was ascertained by 1H-NMR analysis. Yield: 87%; 1H-NMR (400 MHz, CD3OD, 22 °C): δ 4.33 (s, 83H, CH a Hb and CHaH b ), 4.25 (m, 43H, –OCH2–), 3.56–3.48 (m, 45H, –CH2N–), 1.24 (bs, J = 30.2 Hz, 69H, –CH3).
pPr_20: Yield: 80%; 1H-NMR (400 MHz, CD3OD, 22 °C): δ 4.32 (s, 70H, CH a Hb and CHaH b ), 4.23 (m, 39H, –OCH2–), 3.28 (m, –CH2N–, overlapped with residual H2O peak), 2.01–1.88 (m, 38H, –CH2–), 1.24 (bs, 61H, –CH3).
pBut_20: Yield: 81%; 1H-NMR (400 MHz, CD3OD, 22 °C): δ 4.30 (m, 61H, CH a Hb and CHaH b ), 4.18 (m, 36H, –OCH2–), 3.22 (m, 36H, –CH2N–), 1.77–1.64 (m, 69H, –CH2–), 1.23 (bs, 53H, –CH3).
pPen_20: Yield: 85%; 1H-NMR (400 MHz, CD3OD, 22 °C): δ 4.31 (m, 78H, CH a Hb and CHaH b ), 4.16 (m, 43H, –OCH2–), 3.20 (m, 42H, –CH2N–), 1.76–1.60 (m, 86H, –CH2–), 1.52–1.40 (m, 44H, –CH2–), 1.23 (bs, 65H, –CH3).
pCy_20: Yield: 82%; 1H-NMR (400 MHz, CD3OD, 22 °C): δ 4.77 (m, 19H, –CH), 4.37–4.19 (m, 70H, CH a Hb and CHaH b ), 3.45 (m, 20H, –CH), 2.00 (m, 77H, –CH2–), 1.60–1.40 (m, 80H, –CH2–), 1.21 (bs, 58 H, -CH3).
pPh_20: Yield: 70%; 1H-NMR (400 MHz, CD3OD, 22 °C): δ 7.38–7.04 (m, 71H, Ph –CH), 4.59–4.34 (m, 72H, CH a Hb and CHaH b ), 1.46–1.32 (m, 55H, –CH3).
pBn_20: Yield: 84%; 1H-NMR (400 MHz, CD3OD, 22 °C): δ 7.28–7.39 (m, 39H, Ph –CH), 7.16–7.03 (m, 40H, Ph –CH), 4.60–4.29 (m, 115H, CH a Hb, CHaH b and –CH2–), 1.47–1.30 (m, 60H, –CH3).
Octanol–water bilayer partitioning
Dansylated guanidinium (pEt_20)21 and ammonium-functionalized polycarbonates were synthesized using a modified dansyl alcohol initiator (Supplementary Figure 6). A stock aqueous solution (in PBS) of the respective polymers was prepared to obtain a final concentration of 50 µM. To a 2-mL Eppendorf tube was added an aqueous solution of the polymer (0.5 mL) and octanol (0.5 mL). A series of sodium laurate (various concentrations of 0.5, 1.0, and 2.0 equiv per cationic group) in octanol solutions was prepared. The bilayer mixture was then vortexed for 30 s, centrifuged (2000 rpm for 1 min) and subsequently photographed under illumination from a UV lamp (wavelength: 365 nm).
Degradation study of pEt_20
The degradation study of pEt_20 was conducted in PBS (pH 7.4) at 37 °C to mimic the physiological environment. PBS buffer was prepared in D2O, which allowed for 1H NMR analysis. The samples were taken out at 24, 48, and 72 h for 1H NMR analysis.
Membrane integrity study
The minimum bactericidal concentration (MBC that leads to 99.9% killing after 2-h treatment) of pEt_20 and polymyxin B against A. baumannii was determined prior to the membrane integrity study. A. baumannii 10073 were suspended in PBS to a concentration of 2 × 109 CFU/mL. The polymer pEt_20 or polymyxin B was added to the bacteria suspension to a concentration of 0.5 × MBC, 1 × MBC, and 2 × MBC. The untreated bacteria suspension was employed as control. The samples were incubated at 37 °C for 2 h, and were then filtered with a 0.22 μm filter to harvest the supernatant. The supernatant was subsequently measured for its absorbance using the Thermo Scientific NanoDrop 2000 spectrophotometer based on UV absorption at 260 nm. Each assay was performed in triplicates, and the data were normalized against the absorbance of supernatant of the untreated cells in PBS. The experiment was independently repeated three times.
Drug resistance study and gene sequencing
Drug resistance was induced by repeatedly treating A. baumannii 10073 with imipenem or pEt_20 at sublethal doses50. The MIC of imipenem and pEt_20 against the bacteria at each passage was measured using the broth microdilution method described in Supplementary Methods. A. baumannii 10073 exposed to sub-MIC concentrations (1/2 of MIC at that particular passage) were allowed to regrow and reach a logarithmic growth phase before being used for MIC measurement of the subsequent passage. Development of drug resistance in A. baumannii was evaluated over 30 passages by recording changes in the MIC normalized to that of the first passage.
After treatment with pEt_20 or imipenem for 30 passages, the RNA samples of the bacteria at 30 passages and the first passage were extracted, sequenced, and analyzed. Briefly, total RNA was isolated from bacteria using the Trizol (Invitrogen) according to the manufacture’s protocol. The purity of RNA samples was assessed using the ND-1000 Nanodrop. RNA integrity was evaluated using the Agilent 2200 TapeStation (Agilent Technologies, USA) and each sample has the RINe above 7.0. Then, rRNAs were removed from the total RNA using Epicentre Ribo-Zero rRNA Removal Kit (Illumina, USA) and fragmented to approximately 200 bp. The purified RNAs were subsequently subjected to first-strand and second-strand cDNA synthesis, followed by adapter ligation and enrichment with a low cycle according to the instructions from TruSeq® RNA LT/HT Sample Prep Kit (Illumina, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit®2.0 (Life Technologies, USA) and then diluted to 10 pM for cluster generation in situ on the HiSeq2500 pair-end flow cell, followed by sequencing (2 × 100 bp) on HiSeq2500.
RNA-seq data analyses
Paired-end fastq files were mapped using BWA-MEM51 to the curated reference MDR strain AB030 [NZ_CP009257.1]. Mapped reads were counted using HTSeq52. EdgeR Bioconductor package53 was used to assess differential gene expression. All samples in each comparison (imipenem treatment versus control or pEt_20 treatment versus control) were filtered for lowly expressed/low coverage genes, i.e., rows with counts per million values (CPM) less than 1 were removed and the library size was adjusted accordingly before normalization. Differential gene expression was assessed after modeling for variability in the data using moderated tagwise dispersion. P values are adjusted for multiple hypothesis testing using Benjamini–Hochberg method and genes with adjusted p values <0.05 were considered significant. Significant genes with at least a Log2-fold change of 1 are presented in Supplementary Data 1 (imipenem/control) and Supplementary Data 2 (pEt_20/control), respectively.
In vitro immunogenicity test
Peripheral blood mononuclear cells (PBMCs) were isolated by a standard Ficoll-Hypaque density centrifugation technique (Ficoll-paque Plus, GE Healthcare, Cat #10255485). Briefly, blood of 6–8 weeks old C57BL/6 mice was collected in 1.5 mg/mL EDTA-K2 solution in the presence of the anti-coagulant heparin. The samples were mixed with RPMI1640 medium (1:1). Having added Ficoll-Paque (1:1 ratio), we centrifuged the solution at 500 × g for 30 min and carefully aspirated mononuclear cell layer. Cells were washed twice by RPMI1640 and were re-suspended in medium at a density of 4 × 106 cells/mL and cultured in 24-well plates (2 × 106 cells per well) in 5% CO2 incubator at 37 °C for 4 h. Then, cells were gently washed for three times by RPMI1640 medium to remove non-adherent cells. PMBCs were numerated and recultured at 3 × 10 5/well in 96-well plate and treated with pEt_10 and pEt_20 (100 μg/mL) as well as LPS (100 ng/mL). After 2 days of treatment, cell supernatants were collected to measure IFN-γ and TNF-α by ELISA (Invitrogen, Cat #4337757 and Cat #4341109).
ICR mice (female, 7 weeks old) and C57BL/6J mice (6–8 weeks old) were used in the in vivo studies. Immunosuppression was induced by intraperitoneal injection of 200 mg cyclophosphamide per kg of body weight 4 days before the injection of bacteria. The animal study protocols were approved by the Institutional Animal Care and Use Committee at the University of North Dakota.
Evaluation of LD50 and LD5
The ICR mice were randomly divided into five groups (six per group). Each of the mice was injected intraperitoneally (i.p.) with pEt_10 or pEt_20 at different doses (i.e., 42.0, 43.0, 44.0, 45.0, and 46.0 mg/kg, 0.2 mL/20 g). LD50 and LD5 values were estimated from the survival rate of treated mice over 7 days using the BLISS method54.
Evaluation of ED50 and ED95
The ED50 and ED95 values of pEt_10 and pEt_20 were evaluated in MDR A. baumannii 10073-, E. coli 56809-, K. pneumoniae 8637-, and MRSA 25312-induced peritonitis mouse models (ICR mice). Overnight cultures of A. baumannii 10073, E. coli 56809, K. pneumoniae 8637, and MRSA 25312 were suspended in PBS. The cyclophosphamide-pretreated mice were injected intraperitoneally (i.p.) with bacterial suspension at various doses (A. baumannii 10073: 8.0 × 107, 1.6 × 108, 2.4 × 108, 3.2 × 108 and 4.0 × 108 CFU/mL; E. coli 56809: 2.0 × 106, 4.0 × 106, 6.0 × 106, 8.0 × 106, and 1.0 × 107 CFU/mL; K. pneumoniae 8637: 6.7 × 107, 1.0 × 108, 1.5 × 108, 2.3 × 108 and 3.5 × 108 CFU/mL; MRSA 25312: 1.0 × 108, 2.0 × 108, 4.0 × 108 and 6.0 × 108 and 8.0 × 108 CFU/mL, 0.5 mL via i.p. injection; Six to eight mice per group). The minimum lethal dose, which was enough to cause 100% mortality, was determined based on the survival rate of infected mice at 48 h post infection using the BLISS method54.
To evaluate ED50 and ED95 of the polymers, the immunosuppressed mice were infected with A. baumannii 10073, E. coli 56809, K. pneumoniae 8637, or MRSA 25312 by injecting the corresponding bacterial suspension at the minimum lethal dose to mice intraperitoneally (A. baumannii 10073: 2.7 × 108 CFU/mL; E. coli 56809: 8.8 × 106 CFU/mL; K. pneumoniae 8637: 2.6 × 108 CFU/mL; MRSA 25312: 5.2 × 108 CFU/mL; 0.5 mL per injection). The mice were divided into four groups (six to eight mice per group), and injected with PBS, pEt_10, and pEt_20 and imipenem i.p. at 1 and 6 h or 3 and 8 h post infection at various doses (A. baumannii 10073 infection: 0.02, 0.05, 0.1, 0.2, and 0.5 mg/kg for the polymers; 0.1, 0.2, 0.5, 1.0, and 2.0 mg/kg for imipenem. E. coli 56809 infection: 0.1, 0.2, 0.5, 1.0, and 2.0 mg/kg for the polymers and imipenem. K. pneumoniae 8637 infection: 0.5, 1.0, 2.0, 4.0, and 8.0 mg/kg for the polymers; 2.0, 4.0, 8.0, 16.0, and 32.0 mg/kg for imipenem. MRSA 25312: 0.5, 1.0, 2.0, 4.0, and 8.0 mg/kg for the polymers and vancomycin. 0.2 mL/20 g per injection). The BLISS method was employed to estimate ED50 and ED95 based on the survival rate of the infected mice over 2 days54.
Bacteria count in the blood, abdominal cavity, and organs after polymer treatment
The immunosuppressed ICR mice were injected i.p. with 0.5 mL of A. baumannii 10073 or E. coli 56809 suspension at 2.0 × 108 and 8.0 × 106 CFU/mL, respectively. The mice were then divided into four groups, and injected with PBS, pEt_10, pEt_20, and imipenem at doses that saved 95% mice (ED95) (A. baumannii 10073 infection: 0.28, 0.15, and 2.7 mg/kg, respectively; E. coli 56809 infection: 3.3, 1.8, and 1.1 mg/kg, respectively) at 1 and 6 h post infection. At 24 h post infection, five mice in each group were killed to obtain blood, peritoneal fluid, and organ samples. For taking peritoneal fluid sample, 3.0 mL of PBS was injected into the peritoneal cavity, and the abdomen area was then gently massaged. Peritoneal fluid (2.0 mL per mouse) was recovered from the peritoneum of each mouse after the abdomen was opened. Blood and peritoneal fluid samples were diluted and plated on MH agar plates. At the same time, liver, spleen, and kidneys were removed and homogenized in 2.0 mL of PBS. The homogenate was diluted and plated on MH agar plates. After overnight incubation at 37 °C, the number of bacterial colonies was counted. For the organs, the data are presented as lg (CFU/mL of homogenate).
CLP-induced polymicrobial peritonitis mouse model
C57BL/6J mice (male and female, 6–8 weeks, 18–22 g, Jackson Laboratory) were grouped randomly (WT-without surgery or any treatment, Sham-with surgery but without CLP, CLP, CLP-pEt_20, CLP-gentamicin, 6 mice in each group). Mice were fed with normal diet for 12 h before surgery. Then mice were anesthetized with intramuscular (i.m.) injection of ketamine (40 mg/kg of body weight), and the abdominal area was disinfected. The cecum was exposed, ligatured at its external third, and punctured with 27-gauge needle. The abdominal musculature and abdominal skin were closed by applying simple suture. NaCl solution (0.9%, 1 mL) was injected i.p. to supplement the lost moisture. After surgery, the mice were allowed to drink freely. In the Sham group, the cecum was only exposed but not punctured, and was then returned to the abdominal cavity. At 1 h post-CLP (all mice were able to move normally), mice were injected i.p. with pEt_20 and gentamicin solution at 25 and 10 mg/kg, respectively. Another dose was given at 6 h post-CLP. The mice were observed for survival using Kaplan–Meier curves [n = 6, p = 0.0112 (<0.05), Log-rank test].
In another experiment (three mice in each group), mice were killed at 24 h post-CLP. Blood and peritoneal fluid were collected for analysis of bacterial load.
Lung infection model
C57BL/6J mice (male and female, 6–8 weeks, 18–22 g, Jackson Laboratory) were grouped randomly (control without infection or any treatment, infection without any treatment, pEt_20 treated and imipenem treated, 6 mice in each group). Lung infection was established by intranasal instillation of P. aeruginosa PA14 strain [a clinically isolated sample, kindly provided by George A O’Toole (Dartmouth Medical School); 1 × 107 CFU]. The infected mice were treated with pEt_10 and imipenem at 1, 6, and 25 h post infection (8 mg/kg of mouse body weight per injection for pEt_10 and 15 mg/kg of mouse body weight per injection for imipenem). The mice were monitored for survival using Kaplan–Meier curves [n = 6, p = 0.0214 (<0.05), Log-rank test].
In another experiment (three mice in each group), mice were killed at 72 h post infection. Blood and the lung were collected for analysis of bacterial burden.
Biodistribution of pEt_20
The experiments were conducted in accordance with the approved protocol from the IACUC at the Biological Resource Centre of Singapore. Balb/c mice, with an average weight of 20 g, were used for this study. The mice were divided into two groups and administrated with 1.3 mg/kg AF750-conjugated polymer (synthesis in Supplementary Information) (in 200 µL of sterile PBS) by i.v. or i.p. injection. The mice were killed at 4, 24 and 48 h post administration and organs including the brain, heart, liver, spleen, lungs, and kidneys were excised and imaged using IVIS (Caliper Life Science, USA). The near-infrared fluorescence was imaged using the ICG filter pairs and exposure time was set to 3 s.
Pharmacokinetics of pEt_20
The experiments were conducted in accordance with the approved protocol from the Institutional Animal Care and Use Committee (IACUC) at the Biological Resource Centre of Singapore. Balb/c mice (n = 5) with an average weight of 20 g was injected with the AF750-conjugated pEt_20 solution (1.3 mg/kg in 200 µL of sterile PBS) as a single bolus into the tail vein. Blood was collected prior to administration of pEt_20 and at 2, 10, 15, 30, 60, 120, 240, and 480 min thereafter, and plasma was harvested by centrifugation at 1000 × g. Plasma samples were stored at −80 °C until analysis. The concentrations of AF750-conjugated pEt_20 in the samples were determined by measuring the fluorescence intensities at Ex/Em 749 nm/775 nm.
Analyses for difference between the treatment and control arms were performed using one-way analysis of variance (ANOVA) and post hoc Tukey’s test. P values of <0.05 were considered significant. Statistical calculations were performed using SPSS software.
Data supporting the findings of this study are available within the article (and its Supplementary information files).
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This work was financially supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council and ARP-DG 001–005, Agency for Science, Technology and Research, Singapore), and IBM Almaden Research Center (USA). W.C. and Z.C.L. acknowledge the support of National University of Singapore (NUS) Graduate School for Integrative Sciences & Engineering through a Ph.D. Scholarship.