Article | Open

Ancient environmental DNA reveals shifts in dominant mutualisms during the late Quaternary

  • Nature Communicationsvolume 9, Article number: 139 (2018)
  • doi:10.1038/s41467-017-02421-3
  • Download Citation
Received:
Accepted:
Published online:

Abstract

DNA-based snapshots of ancient vegetation have shown that the composition of high-latitude plant communities changed considerably during the late Quaternary. However, parallel changes in biotic interactions remain largely uninvestigated. Here we show how mutualisms involving plants and heterotrophic organisms varied during the last 50,000 years. During 50–25 ka BP, a cool period featuring stadial-interstadial fluctuations, arbuscular mycorrhizal and non-N-fixing plants predominated. During 25-15 ka BP, a cold, dry interval, the representation of ectomycorrhizal, non-mycorrhizal and facultatively mycorrhizal plants increased, while that of N-fixing plants decreased further. From 15 ka BP, which marks the transition to and establishment of the Holocene interglaciation, representation of arbuscular mycorrhizal plants decreased further, while that of ectomycorrhizal, non-mycorrhizal, N-fixing and wind-pollinated plants increased. These changes in the mutualist trait structure of vegetation may reflect responses to historical environmental conditions that are without current analogue, or biogeographic processes, such as spatial decoupling of mutualist partners.

Introduction

Biotic interactions, including mutualisms, play an important role in determining species’ responses to environmental change and thus mediate community composition and diversity. Through time, both plant life-history traits1 and biotic interactions are modified by changes in climate2. Until now, most attention has been paid to herbivory and specifically its dynamic relationship with vegetation composition.3,4 Much less is known about the stability, or otherwise, of mutualisms through time. However, the spatial and temporal scales over which such relationships change is an important additional key to understanding ecosystem responses to future climate change5. Palaeodata offer important insights into the long-term trajectory of interactions and the longevity of ecosystems6. Conceivably insights into key plant mutualisms can be gained from examining floristic composition and related plant traits during the climate changes of the late Quaternary.

Most records of past vegetation derive from fossil pollen, to the extent that records have been collated into comprehensive databases7,8. However, many records from north-temperate and boreal regions are dominated by wind-pollinated taxa that are high pollen producers, weighting the signal against insect-pollinated taxa and underestimating species richness in records based on relatively low pollen counts9. Macrofossils can provide a greater level of taxonomic resolution than many pollen records, but they are less common, they provide localized snapshots of palaeovegetation and their preservation varies depending upon the durability of the plant tissue10. Metabarcoding of ancient DNA is an alternative approach to studying past vegetation11 that has the potential to produce taxonomically rich records12. While it has limitations, such as poor resolution of some taxonomic groups13 and representational biases among functional groups14, ancient DNA occurs in most depositional environments, and its analysis can potentially reveal those fractions of plant communities and local floras not reliably retrieved by analysis of pollen or plant macrofossils.

Recently, Willerslev et al.12 generated an extensive environmental DNA (eDNA) data set describing the floristic composition of vegetation at sites across the largely unglaciated northern high latitudes (Siberia, Alaska and Yukon) during the last 50,000 years. They assigned samples to three time periods, representing a compromise that allowed robust statistical comparisons but also reflected key changes in climatic conditions pertinent to the study region, which differed from those of glaciated Eurasia and North America (15,16; see Methods). Because the period 25 to 15 ka BP was comparatively climatically stable (cold and arid) in the study region, Willerslev et al.12 designated it the last glacial maximum (LGM). The pre-LGM period (>25 ka BP) was characterized by fluctuating stadial-interstadial conditions17, while after 15 ka BP (post-LGM), a major climatic transition affected the region, bringing moister and warmer conditions that were a precursor to the establishment of the current interglacial period (18; Holocene; 11.4 ka BP-present). Willerslev et al.’s12 results indicated the existence of taxonomically rich pre-LGM vegetation that was dominated by forbs and encompassed taxa that typify both tundra and arctic steppe environments. Taxonomic diversity declined during the LGM, and the LGM flora was largely a subset of the pre-LGM flora. Post-LGM diversity increased, without reaching the pre-LGM level, and comprised fewer forbs and more graminoids and trees than in earlier periods, reflecting a shift from dry tundra-steppe to moist tundra and forest. Willerslev et al.12 linked vegetation structure and composition with megafaunal diet but did not consider other biotic interactions, such as mutualisms. Here, we investigate the representation of mutualisms associated with late-Quaternary vegetation, focusing on three types of mutualistic interaction between plants and heterotrophic organisms: mycorrhizal associations, symbiotic nitrogen fixation, and pollination.

Mycorrhizae are an ancient symbiosis between soil fungi and plant roots. Several types of mycorrhizal symbiosis are recognized, of which three are widespread. Arbuscular mycorrhizae (AM) are the most ancient type and also the commonest, with > 80% of vascular plant species capable of entering into the symbiosis. Ectomycorrhizae (ECM) and ericoid mycorrhizae (ERM) have evolved multiple times, all more recently than AM, and are only formed by approximately 2 and 1% of vascular plant species, respectively. Non-mycorrhizal status (NM), which is also evolutionarily recent, can be attributed to about 6% of plant species19,20.

With increasing latitude or altitude, predominantly AM vegetation (grassland, forest or shrubland) is replaced by ECM forests with AM understory, then by ECM forests with ERM understory, and finally by ERM dominated heathlands and tundra heaths. One might expect that temporal change in the predominance of mycorrhizal types should follow a broadly analogous pattern to those observed in relation to latitude and altitude, e.g. such that the predominance of mycorrhizal types in historically cooler periods most closely resembles the pattern in contemporary high-latitude vegetation. However, actual data about the occurrence during the late Quaternary of plants with particular mycorrhizal characteristics are scarce and concern differential extinction21 and migration22,23 of AM and ECM trees during the Holocene.

If a plant species is involved in mycorrhizal symbiosis, its mycorrhizal status—whether it is obligately mycorrhizal (OM) or facultatively mycorrhizal (FM)—provides an indication of its reliance on the symbiosis19,24. In Europe, at the regional scale, OM plant species are known to prefer drier and warmer habitats with higher soil pH, while FM species have broader distributions25,26. The benefit of mycorrhizal association may change in relation to CO2 concentration27, but there is no empirical information about mycorrhizal function in relation to historically lower CO2 concentrations or under conditions of carbon starvation. Nor is there information on how the shares of NM, OM and FM plant species have changed through the late Quaternary.

Symbiotic nitrogen fixation reflects the ability of some plant species to fix gaseous N2 from the air with the help of i) bacteria collectively referred to as rhizobia, ii) the actinobacteria genus Frankia or iii) cyanobacteria28,29. In certain conditions, plants hosting symbiotic nitrogen-fixing microbes are major ecosystem drivers30,31.The local abundance of the N-fixing symbiosis depends on temperature, soil resources and other biotic interactions29, while, at large scales, the rate of biological N-fixation depends on climatic parameters and CO2 concentration29. In current vegetation the share of N-fixing plants in communities tends to decline with increasing latitude32. However, studies describing variations through geological time in the relative abundance of N-fixing plants are scarce (but see31), and we are unaware of any studies relating to the late Quaternary.

Alongside predominantly trophic interactions, such as mycorrhiza and biological N-fixation, plant-pollinator relationships represent an important class of mutualistic interaction. The proportion of animal-pollinated species among Angiosperms varies from 78% in the temperate zone to 94% in the tropics33. Empirical information about the geographic distribution of pollination modes remains limited. Some studies have shown that the proportion of particular pollination modes in plant communities may depend on altitude and average wind speed34,35. Dalsgaard et al.36 modelled the impact of historical climate change on current plant-pollinator networks, but we are unaware of similar studies using empirical palaeoecological data. Indeed, it has generally been difficult to assess the relative abundance of different pollination modes in palaeovegetation because wind-pollinated species predominate in pollen records. Using DNA-based techniques to estimate the proportions of wind-pollinated plants in late Quaternary vegetation could provide an important insight into potential biases affecting pollen-based analyses.

Here, we integrate available data on plant mutualistic traits with Willerslev et al.’s12 data set to investigate how the abundance of plants associated with different symbioses changed during the late Quaternary. Contemporary correlates of mutualist trait abundance provide some basis for making general predictions about temporal patterns during the late Quaternary. Since most traits have been shown to vary in relation to latitude we hypothesized that trends in the relative abundance of mutualist types through warmer and cooler periods of the late Quaternary might broadly reflect patterns seen along contemporary latitudinal gradients. However, we note that modern analogues for past vegetation (and associated mutualisms) are incomplete, reflecting the large difference in climatic conditions between glacial and interglacial periods37,38. Indeed, our analyses show that changes in the relative abundance of mycorrhizal types and statuses, N-fixing plants and insect-pollinated plants in late-Quaternary vegetation do not consistently reflect patterns seen along contemporary latitudinal gradients. Instead, alternative patterns of mutualistic relationship may have been driven by the direct or interactive effects of non-analogous environmental conditions or by biogeographic processes accompanying climate changes.

Results

Trait abundance and correlation

Among the molecular operational taxonomic units (MOTUs) retained for analysis, 86 (66%) were forbs, 28 (21%) were graminoids, 11 (8%) were dwarf shrubs and 6 (5%) were trees or shrubs; 86 (66%) were AM, 7 (5%) were ECM, 10 (8%) were ERM and 28 (21%) were NM; 58 (44%) were FM and 45 (34%) were OM; 9 (7%) formed N-fixing symbioses and 122 (93%) did not form N-fixing symbioses; 87 (66%) were insect-pollinated and 44 (34%) were wind-pollinated.

Mycorrhizal traits are known to be related to growth form19, and our data confirmed these correlations (Table 1). For instance, we recorded relatively more trees and shrubs among ECM and ERM species, which in turn tended to be obligately mycorrhizal species. We also recorded varying degrees of correlation between all other traits (Table 1). For instance, there were no NM species among N-fixers in this data set.

Table 1 Correlation between mutualist traits and growth form in a taxon list of 131 plant MOTUs recorded in northern high-latitude permafrost samples12

Community-level trait responses to climate

The growth form composition of vegetation across the study region, as estimated from the proportion of DNA sequence reads representing the different categories, varied between climatic periods (Fig. 1 and Table 2; see ref. 12). The post-LGM was characterized by fewer forb reads and more reads corresponding to other growth form categories, compared with both of the earlier periods. The proportions of taxa falling into the different growth form categories followed a similar pattern (Fig. 1).

Fig. 1
Fig. 1

Functional structure of past northern high-latitude vegetation. Representation of growth forms, mycorrhizal types, mycorrhizal statuses, pollination types and N-fixing taxa among plants recorded from permafrost samples (n = 216) dated to three climatic periods (pre-LGM, n = 145; LGM, n = 32; post-LGM, n = 39) during the late Quaternary. The proportional abundance of DNA sequence reads and MOTUs (molecular operational taxonomic units) is shown. Error bars show bootstrapped 95% confidence intervals

Table 2 Variation in the relative abundance of sequencing reads corresponding to different trait categories (Reads) and in the relative abundance of taxa in different trait categories (MOTUs) in relation to climatic period (pre-LGM, LGM, post-LGM)

The relative abundance of mycorrhizal types, expressed either as the proportion of sequence reads or the proportion of MOTUs, was significantly related to climatic period (Fig. 1 and Table 2). In both analyses, the relative proportions of mycorrhizal types differed significantly between pre-LGM and post-LGM, while the LGM was intermediate (Table 2). During the pre-LGM, AM host plants predominated, with other mycorrhizal types present at lower abundance. During the LGM, the shares of AM plants decreased while the shares of ECM plants and NM plants increased. In the post-LGM climatic period, the proportions of AM and ERM plants decreased, while the shares of ECM and NM plants increased.

The relative abundance of plants exhibiting different mycorrhizal statuses was also significantly related to climatic period, both when considered as the number of sequence reads or the number of MOTUs (Fig. 1 and Table 2). The proportions of reads corresponding to different mycorrhizal statuses differed between all climatic periods, while the proportion of MOTUs differed significantly between pre-LGM and post-LGM, with the LGM intermediate (Table 2). Pre-LGM was characterized by a relatively high proportion of OM plants, while in later stages the proportions of FM (particularly LGM) and NM (particularly post-LGM) plants increased.

The share of N-fixing plants in vegetation was lower in the LGM than during both pre-LGM and post-LGM (though not significantly different from pre-LGM for the proportion of reads; Fig. 1 and Table 2), while the abundance of different pollination modes did not vary significantly between climatic periods (Fig. 1 and Table 2).

Most trait categories exhibited positive spatial autocorrelation in trait composition at 0–200 km and negative autocorrelation at >2000 km (Supplementary Fig. 1). However, the magnitude of correlation was low and had a minor influence on predicted type I error rates in our analyses (Supplementary Fig. 2 and Supplementary Table 1). The results of most trait–climate models were consistent with a scale-independent trait–climate relationship; though some results relating to growth form and mycorrhizal type were ambiguous (Supplementary Fig. 3).

Traits associated with MOTU responses to climate change

We used an ordination approach—the outlying mean index (OMI)—to approximate the position of MOTUs in a climatic niche space defined by the pre-LGM, LGM and post-LGM periods. Niche positioning is calculated as the distance between the mean environmental conditions used by the taxon (its occurrence in different periods) and the mean conditions of the study as a whole (i.e., reflecting the extent to which different periods were sampled39). The average OMI of individual MOTUs was significantly greater than expected at random (P < 0.01), supporting the positioning of MOTUs on the ordination plot (Fig. 2). When traits were considered in isolation, growth form, mycorrhizal type, mycorrhizal status and pollination mode all had significant power to explain MOTU niche locations (Table 3). After accounting for the effects of growth form and other non-confounded mutualist traits (mycorrhizal type and mycorrhizal status were strongly correlated and partially confounded, so were not included in the same model), only mycorrhizal status retained significant explanatory power.

Fig. 2
Fig. 2

Positioning of northern high-latitude plants in historical climatic niche space. The OMI constrained ordination approach is used to estimate the niche position of plant species (points) as defined by their abundance in three climatically different periods during the late Quaternary: pre-LGM, LGM and post-LGM. Symbol sizes are scaled to represent the density of data points at identical positions in ordination space

Table 3 Power of mutualist traits and growth form to predict the climatic niche of 131 plant MOTUs in northern high-atitude permafrost samples15

The position of MOTUs differed significantly between trait categories along the first OMI axis (pre-LGM/LGM vs post-LGM) but not along the second (pre-LGM/post-LGM vs LGM) (Fig. 3). The average niche position of forb MOTUs was located significantly closer to the pre-LGM/LGM and further from the post-LGM (axis 1) than all other growth forms. The average niche position of AM MOTUs mirrored that of forbs, being located significantly closer to the pre-LGM/LGM and further from the post-LGM (axis 1) than all other mycorrhizal types. FM MOTUs were also located closer to the pre-LGM/LGM (axis 1), and NM closer to the post-LGM (axis 1), compared with other mycorrhizal statuses. MOTUs belonging to different N-fixing categories did not differ in average niche position along either axis. Wind-pollinated MOTUs were located closer to the post-LGM and insect-pollinated MOTUs closer to the pre-LGM/LGM.

Fig. 3
Fig. 3

Outlying mean index (OMI) axis scores of plants with different mutualist traits. OMI axis scores represent an estimate of species’ niche positioning. The first axis corresponds to the distinction between post-LGM (more negative values) and earlier periods (more positive values), while the second corresponds to the distinction between LGM (more negative values) and other periods (more positive values). The mean value (±SE) for each trait category is presented

The estimated positioning of different mutualist trait categories in climatic niche space was relatively similar in non-phylogenetic models and in models where phylogenetic signal was co-estimated with other model parameters (Supplementary Fig. 4). Nonetheless, mean positions along axis 1 were generally estimated with less precision by the phylogenetic model, and all categories of mycorrhizal status, N-fixing status and pollination mode showed relatively greater affiliation with the post-LGM in the phylogenetic model.

Discussion

The distribution of mutualist traits in northern high-latitude vegetation has fluctuated during the last 50,000 years. The relatively taxon-rich vegetation of the pre-LGM was dominated by taxa that form AM, with fairly equal abundances of OM and FM taxa. Most taxa were non-N-fixing during this period. During the LGM, plant species richness decreased12, and the mutualist characteristics of the vegetation changed: the representation of AM taxa decreased, while that of ectomycorrhizal (ECM) and non-mycorrhizal (NM) taxa increased, as did the share of FM taxa. The proportion of N-fixing taxa decreased. Plant species richness increased again in the post-LGM, though it did not reach the level of the pre-LGM12. Notably, there was a further decrease in the share of AM taxa and an increase of ECM and NM taxa. The share of N-fixing taxa increased to approximately pre-LGM levels, though there was no association between the N-fixing trait and MOTU climatic niche positioning. Temporal variations in the pollination trait also received inconsistent statistical support: temporal patterns in the share of pollination types were weak, but wind-pollinated MOTUs were significantly associated with the post-LGM. These results indicate weak support for some contemporary environmental correlates as drivers of mutualist change through the late Quaternary, but no support for others. Our hypothesis that trends in the abundance of mutualisms during cool and warm periods of the late Quaternary would mirror patterns seen along contemporary latitudinal gradients was generally not supported.

Both physical data40, data-model comparisons41 and ecosystem function reconstructions taking into account the extinction of megafauna4 attest that conditions in the study region for much of the late Quaternary do not have direct analogues today16,37,38,42. Below we consider how such non-analogous aspects of the climate and environment may have contributed to the results we observed. We also consider whether biogeographic processes and biotic interactions within the wider ecosystem might have played a role. While we cannot explicitly address the mechanisms underlying the observed patterns on the basis of descriptive data, we propose what we believe to be plausible explanations.

Trends in the abundance of mycorrhizal types across warmer and colder periods of the late Quaternary did not mirror patterns seen along contemporary latitudinal and elevational gradients. We observed a shift from AM to ECM and NM in the post-LGM, but, interestingly, not to ERM, even though Ericales are most dominant in post-LGM pollen records43. Trends in precipitation may be an important driver of these changes. High soil moisture and low temperature are associated with NM status and low representation of AM species in contemporary northern-hemisphere vegetation25,26,44. Increasing precipitation across the study region from ca. 14 ka BP onwards16,45,46 resulted in higher soil moisture, making the post-LGM the wettest of the three periods. This shift is therefore consistent with the observed NM increase and AM decrease in the post-LGM. The changing level of atmospheric CO2 may also have played a role, given the strong increase in CO2 concentrations from as low as 190 ppm during the LGM to ambient post-industrial levels of 300–400 ppm47. The efficiency of AM associations has been shown to decrease with increasing CO2 (34), while ECM plants exhibit a strong positive response to elevated CO227.

Plant associations with mycorrhizal fungi and N-fixing microorganisms may also have been influenced by variation in the availability of potentially limiting nutrients. Nitrogen is thought to be the most limiting nutrient for plant productivity in many contemporary northern ecosystems, although co-limitation by P is not uncommon48,49,50. In general, AM particularly enhances plant P nutrition, while ECM allows plants to obtain N19. On this basis, the observed relative abundances of these mycorrhizal types suggest that P-limitation in pre-LGM vegetation may have been replaced by N-limitation in post-LGM vegetation. Increasing precipitation with the onset of interglacial conditions led to the development of more organic soils and to paludification in some parts of the study area51. These changes are likely to have offset, at least in part, increases in mineralization of both N and P with rising temperatures52, although the effect may have been dependent on vegetation type53. Aerts et al.49 showed experimentally that moderate changes in summer temperatures stimulate litter P release while having limited effects on litter N release in sub-arctic ecosystems. As a result, N-limitation of plant growth in such ecosystems may be promoted by warming. While no direct evidence of a transition from P-limitation to N-limitation during the last 50 ka exists, a global comparison of sedimentary nitrogen isotopes and carbon accumulation across the glacial–deglacial transition led McLauchlan et al.54 to conclude that terrestrial nitrogen availability generally declined as global climates warmed at the onset of interglacial conditions.

Because the magnitude of biological nitrogen fixation is positively related to ecosystem evapotranspiration16, and considering that CO2 levels and soil C:N ratios were lowest in the LGM54, the share of N-fixing plants might be expected to be moderate during the pre-LGM, low during the LGM and highest during the post-LGM. The recorded fluctuation in the share of N-fixing plant taxa was partially consistent with this because the total share of N-fixing plants was slightly higher during the post-LGM.

Warmer regions of the globe tend to harbour higher proportions of insect-pollinated plants55, so it might be expected that warmer climatic periods have also been associated with higher levels of insect pollination. However, the contemporary latitudinal gradient incorporates a larger temperature range than the temporal gradient of the late Quaternary. Indeed, we found that the proportion of insect-pollinated plants did not vary between climatic periods, and ordination analysis suggested that, if anything, wind pollination was more associated with the post-LGM. However, pollination traits were not strongly associated with plant climatic niche positioning once growth form differences were accounted for. Analogous results were recorded by Moeller et al.56 in relation to a contemporary latitudinal pattern. Indeed, many ectomycorrhizal trees and non-mycorrhizal sedges, which increased during the post-LGM, are wind pollinated. The tendency for wind pollination to increase in the post-LGM may indicate that pollen-based analyses capture a larger fraction of existing diversity in this period compared with earlier periods.

Besides important changes in the abiotic environment, the late Quaternary has been characterized by dynamic patterns of organism distribution, including individualistic species responses to climate change37 and the extinction of certain megaherbivores4. The onset of the post-LGM represents a period when temperate organisms recolonized higher latitudes and boreal floras occupied many areas previously dominated by arctic floras. This spatial reorganization was reflected in high temporal rates of compositional change in plant communities in eastern North America37,57 and Europe58. The post-LGM vegetation communities investigated here also exhibited high spatial turnover and differed from those that had been locally present during either the pre-LGM or the LGM12. Among the biological communities that (re)colonized local sites as the climate warmed during the post-LGM, some potential mutualistic partnerships may not have re-formed if the taxa involved possessed differing dispersal abilities. Analogous disconnects between potential partners can occur during primary succession when plants and AM fungi colonize new sites59,60, but it is unclear to what extent such processes occur over much larger temporal and spatial scales22,23. Nonetheless, the high proportions of NM and FM taxa in the post-LGM are consistent with this scenario.

Major vegetation changes marking the transition to interglacial conditions were broadly coincident with the extinction of megaherbivores in northern Eurasia and North America4,42. Changes in the mutualist trait structure of vegetation during the post-LGM—such as the increase of ECM woody plants and decrease of predominantly AM forbs—may have been to some extent related to a reduction in herbivory and disturbance. Nonetheless, it is notable that mycorrhizal status remained a significant predictor of species responses to climatic change even after accounting for the effect of growth form.

Incorporation of phylogeny into models decreased the precision of estimated trait–environment relationships. Where phylogenetic and non-phylogenetic models suggest similar relationships but with stronger effects in the non-phylogenetic model, this may indicate that trait and niche are to some degree phylogenetically conserved, but that some differentiation is apparent within recently emerged clades61. If similarly conserved but unmeasured traits were critical to plant survival under late-Quaternary conditions, such traits might also underlie the patterns observed here. However, we note that mycorrhizal traits—specifically mycorrhizal status—were significant predictors of taxon responses to climate change, even when the effects of other mutualist traits and growth form were accounted for. Therefore, we consider the evidence for a direct link between mycorrhizal traits and taxon responses to be the strongest to emerge from these analyses, although responses associated with other traits should not be discounted.

Our results make the implicit assumption of scale-independent correlation between environment and traits, i.e., a consistent relationship throughout the study region. To the degree that we were able to assess this, the data were largely consistent with this assumption. However, it should be acknowledged that the available sample set provided spatially and temporally uneven coverage12, which, allied with the moderate total sample size, meant that we did not have high power to detect short-term or regional variations. We therefore do not dismiss the possibility that mutualisms across different regional biotas within the study area responded differently to the environmental changes of the last 50,000 years.

Many forecasts of the impact of climate change on biodiversity are made using macroecological or species distribution models62 that incorporate information about current relationships between organisms and the abiotic environment, usually climate parameters. The results of this study show that temporal changes in the abiotic environment were paralleled not only by shifts in floristic composition but by changes in the structure of mutualist relationships among late-Quaternary plant communities. In general the patterns of mutualist trait abundance seen along contemporary latitudinal gradients did not make good predictors of variations in trait abundance between the cooler and warmer periods of the late Quaternary. We have attempted to provide plausible ad hoc explanations for the observed patterns. The complexity of observed responses, even based on a relatively small suite of traits, indicates how features not directly related to climate may be important in predicting trait distributions, and hence floristic composition, in communities.

Methods

Ancient DNA data set

We used data from Willerslev et al.12, who identified plant species or species groups (molecular operational taxonomic units; MOTUs) from 242 permafrost sediment samples collected from 21 sites in the northern high latitudes. Of these, 221 samples came from northern Siberia, Alaska and northwest Canada, regions left largely ice-free during the last glacial maximum that preserve older deposits. Sample radiocarbon ages ranged from modern to approximately 50 ka BP63. Some of the oldest sample ages (40–50 ka BP) may actually be infinite, though most samples are stratigraphically consistent with an MIS-3 designation.

Samples were assigned to a simplified temporal classification relevant to the climate history of the study region: >25 ka BP, 25 to 15 ka BP, <15 ka BP, and given the labels pre-LGM, LGM and post-LGM for ease of reference. Samples dating to the pre-LGM and LGM were largely derived from yedoma, ice-rich silt deposits that accumulated, mostly as a result of nutrient-rich loess deposition40, under cold, dry climates. The sampled deposits were incorporated into the permafrost zone after deposition, but each 100-mm diameter sample represents years of accumulation and may have been subject to bioturbation/cryoturbation over a few centuries prior to freezing, depending upon active-layer depth and sediment accumulation rate (e.g., given a sedimentation rate of 1 mm per yr and active-layer depth 300 mm: a sample would have accumulated over 100 yr and seasonally thawed for 300 yr). Samples were collected away from the contacts between ice wedges and sediment to ensure that material that may have been moved vertically in relation to frost-cracking and ice-wedge growth was not sampled. Although sites are widely separated, from their current landscape position, many localities were sited on low-lying interfluves meaning that the sampled landscape was more homogenous than if all landscape units (e.g., river valleys, mountaintops) were sampled evenly. These landscapes supported a diverse Pleistocene megafauna64. Post-LGM samples were more varied: some were from yedoma, others from lake sediment, peat and soil. This reflects a major rearrangement of northern landscapes and soils, which featured cessation of loess deposition, development of wetlands and organic soils (with a concomitant reduction in nutrient availability), the northward spread of boreal forest into previous treeless vegetation, and the extinction of many megafaunal taxa. While their origins are more varied, the samples nevertheless record the consequences for vegetation composition of this major environmental shift.

Willerslev et al.12 sequenced the P6 loop of the chloroplast trnL (UAA) intron, generating 14,601,839 reads after stringent quality filtering. Reads were assigned to MOTUs present in Arctic and boreal trnL taxonomic libraries13 or GenBank on the basis of sequence similarity (using the programme ecotag65). Rare MOTUs (<5 reads) were omitted12. In total a match was found for 7,738,725 reads and these were presented in Supplementary Table 2 of Willerslev et al.12.

Because the relative number of reads of a given taxon has been shown to be a sufficiently good proxy of its relative cover or biomass in a vegetation sample14,66, Willerslev et al.12 used the quantitative information in their data as a proxy for MOTU abundance. We use the same time period classification and quantitative data in our analysis.


Plant traits

Willerslev et al.12 used information in the BiolFlor database67 to assign MOTUs to the following growth form categories: forbs, graminoids (grasses, sedges and rushes), dwarf shrubs or other woody plants (trees and shrubs). Where MOTUs could only be linked with species groups or higher-level taxa, and all constituent species had the same growth form, this was allocated to the MOTU; otherwise the MOTU was left ‘undefined’. Growth form was allocated to 147 of the 154 identified MOTUs. We used the same approach to compile information on MOTUs for mycorrhizal traits, N-fixation and pollination mode traits from available databases (BiolFlor, Mycoflor26) and additional literature searches.

To assign a category of mycorrhizal type (AM, ECM, ERM and NM) and status (FM, OM and NM) to each plant taxon (NM was considered both in the context of types and status), we compiled mycorrhizal information from published data sets, starting with the most recent before moving to progressively older sources—from Hempel et al.26 to Akhmetzhanova et al.68, Wang and Qiu69 and Harley and Harley70—as the more recent data sets generally cover, update and correct earlier information. This process was done carefully to avoid errors propagating in the literature. We performed a review of the mycorrhizal trait data set, limiting possible sources of error as indicated by Brundrett20 and quality controlling the data as described in Bueno et al.25 Four and six MOTUs were allocated to the ‘undefined’ category for mycorrhizal status and type, respectively.

To identify which MOTU component plant species were involved in N-fixing mutualisms, we used data from the BiolFlor database and an additional literature search. MOTUs were assigned to two categories: involved or not involved in N-fixing mutualism. Four MOTUs were undefined.

We categorized MOTUs according to the recorded pollination mode in the BiolFlor database. We considered only two major pollination modes: (1) wind pollination and (2) insect pollination. Although many arctic plant populations are predominantly self-pollinated, mixed-mating (i.e., combining self-pollination with mostly insect-mediated cross-pollination), or partly or fully agamospermous, this may vary within a species across its range71,72 and there is insufficient information available to classify such species separately in our data set. Because those taxa in our data set that are known to exhibit some degree of self-pollination or agamospermy have insect pollination syndromes (i.e., they are derived from insect-pollinated ancestors), we classified them as insect-pollinated. Nine MOTUs were ‘undefined’.

After compiling the trait database, we removed 14 MOTUs (2226214 reads) that could not be defined for all traits and five samples that no longer contained any MOTU data. This left a data table containing 216 samples (145 pre-LGM, 32 LGM and 39 post-LGM), 131 MOTUs (98 pre-LGM, 40 LGM and 51 post-LGM) and 5026792 reads (2469914 pre-LGM, 469290 LGM and 2087588 post-LGM). The precision of taxonomic and trait composition estimates was similarly high in all periods (Supplementary Fig. 5).


Sensitivity analysis

Plant trait databases (e.g., Biolflor) almost certainly include errors resulting from misidentification of traits and incorrect transfer of information among sources. The level of error among mycorrhizal trait categorizations has been estimated at 10% of species20. We assessed the robustness of our data set to potential errors by introducing random errors into some of the mutualist trait assignments. We assumed that errors may affect assignment of mycorrhizal status, mycorrhizal type (except ERM) and pollination mode; by contrast, we assumed that ERM mycorrhizal type and N-fixing associations, which are phylogenetically conserved, should be reliable. Thus, for each susceptible trait we randomly selected species corresponding to a given error rate (we considered rates between 1 and 20%). For these species, we reassigned the trait category to a randomly selected alternative category. We repeated this process 1000 times at each error rate to generate replicate data sets with a given level of randomly introduced error. We then calculated the proportional abundance of each trait in every sample and correlated these values with the trait abundance observed in the original data. We also recalculated the major analyses described below assuming a 10% error rate in susceptible traits. We found that while trait abundance varied slightly between data sets using original and random trait errors, the trait responses to climatic period remained very similar, with the possible exception of ECM, which was less strongly associated with the post-LGM in data sets incorporating random error (Supplementary Figs. 6 and 7). Also, the results of the most conservative multivariate analysis—the effect of mutualist trait category on species abundance after accounting for growth form and confounded traits—did not change qualitatively in analysis of randomly altered data sets: mycorrhizal status generally remained a significant predictor; original R2 = 0.04; original P = 0.01; median R2 from random analyses = 0.03; median P from random analyses = 0.04.


Data analysis

To assess the abundance of different mutualist traits in different climatic periods (pre-LGM, LGM and post-LGM), we calculated for each sample the relative share of each mutualist category in the MOTU list, as well as the relative abundance of MOTUs in each mutualist category, estimated on the basis of read frequency. The shares of different categories among the total pool of reads recovered from a sample resembles what is known as the community weighted mean in plant community ecology73. We used bootstrapping to estimate confidence intervals around the shares of different categories: we resampled the raw data table 1000 times, randomly selecting samples with replacement within each climatic period category. The 2.5 and 97.5% percentiles of the bootstrapped distributions were used to estimate 95% confidence intervals. For each trait response matrix we used permutational multivariate analysis of variance (PERMANOVA; function adonis in R package vegan with Bray-Curtis distance74) to test the null hypothesis of no difference in trait category distribution (both number of taxa and number of reads per category) between climatic periods.

We also used a constrained ordination approach—the outlying mean index (OMI)—to identify the mutualist traits associated with MOTU-level responses to climatic period. OMI approximates niche positions for species (i.e., MOTUs in this case) by calculating the distance between the mean conditions used by a species and the mean conditions available in a study (i.e., species OMI39). Unlike some other constrained ordination techniques, it is applicable to environmental gradients of any length and is not influenced by variations in site richness. A recent review of methods proposed OMI as a stable and sensitive tool for investigating trait–environment relationships75. We used OMI to generate a constrained ordination of MOTU abundance in response to climatic period (a factor with three-levels: pre-LGM, LGM and post-LGM). The species scores from this ordination thus potentially represented MOTU locations in a two-dimensional ‘climatic’ niche space, with the first axis representing a gradient from pre-LGM and LGM on one hand to post-LGM on the other; and the second axis representing a gradient from pre-LGM and post-LGM on one hand to LGM on the other. We used a Monte Carlo randomization test to test whether average MOTU OMI was greater than would be expected at random (i.e. whether the climatic gradients had significant explanatory power39).

Using the species scores on the constrained ordination axes as the multivariate response variable in PERMANOVA (Euclidian distance), we tested the null hypothesis of no difference in MOTU climatic niche location in relation to mutualist trait category. We constructed models where the effects of single explanatory traits (the mutualist traits plus growth form) were (i) tested in isolation, (ii) tested after accounting for growth form (for mutualist traits); and (iii) tested after accounting for growth form and non-confounded mutualist traits (for mutualist traits; since mycorrhizal type and mycorrhizal status were strongly correlated and partially confounded, these were not entered into the same model). To identify the climatic periods associated with particular trait categories, we tested differences between trait categories in the MOTU scores on the separate individual axes. To test the null hypothesis of no difference between categories, we used Dunn’s non-parametric test, since the residuals from parametric models were not normally distributed.

Willerslev et al.12 demonstrated spatial distance decay in the compositional similarity of plant communities in the permafrost data set. Spatial autocorrelation can lead to inflated Type I error in statistical hypothesis tests, while spatial dependence in trait–environment relationships limits the generality of global estimates of trait–environment correlation76,77. We assessed the degree of spatial autocorrelation and scale-dependence in trait–climate relationships using multi-scale ordination (MSO)77 and Mantel correlograms. Multi-scale ordination is a method based on canonical correspondence analysis that decomposes total, explained and residual variance at different spatial scales. A systematic difference between total variance and the sum of residual and explained variance can indicate scale-dependent species-environment correlation. To construct correlograms, we used distance-based redundancy analysis (Bray-Curtis distance), which is similar to PERMANOVA but allows the calculation of a residual matrix for use in the correlogram. The MSO procedure also generates an estimate of the degree to which residual variance is underestimated as a result of autocorrelation. We used these values to estimate the influence of measured autocorrelation on Type I error rates in PERMANOVA.

Analytical approaches that incorporate phylogenetic relationships between taxa can help to link the evolution of functional traits with adaptation to abiotic or biotic environments. Such ‘phylogenetic correction’ can provide a surrogate of functional trait differences between taxa, and may be particularly important in the presence of missing or complex trait information61. We constructed a phylogeny to represent approximate relationships between the plant MOTUs used in this analysis. To do this, we used a backbone supertree of the European flora78. We first identified MOTUs that corresponded to single plant species that were present in the backbone phylogeny (n = 26). We then grafted terminal branches onto the tree to represent MOTUs that comprised multiple species or higher taxa. For each such MOTU we attached the terminal branch to a node in the phylogenetic tree representing the lowest taxonomic level among genus (75 MOTUs), tribe (7 MOTUs), subfamily (5 MOTUs) and family (18 MOTUs) that comprised all component taxa of the MOTU and was identifiable in the tree. We then pruned all redundant species in the tree to leave a phylogeny containing 131 MOTU tips (Supplementary Fig. 8). We incorporated this phylogeny into a reanalysis of the relationship between MOTU trait categories and MOTU scores along individual OMI ordination axes. We used generalized least squares to simultaneously estimate phylogenetic signal (Pagel’s Lambda) and other model parameters79.


Data availability

The data set of this study is published by Willerslev et al.12. Additional data are available from the corresponding author upon request.

Additional information

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

  1. 1.

    Lacourse, T. Environmental changes controls postglacial forest dynamics through interspecific histories in life-history trains. Ecology 90, 2149–2160 (2009).

  2. 2.

    Blois, J. L., Zarnetske, P. L., Fitzpatrick, M. C. & Finnegan, S. Climate change and the past, present, and future of biotic interactions. Science 341, 499–504 (2013).

  3. 3.

    Gill, J. L., Williams, J. W., Jackson, S. T., Lininger, K. B. & Robinson, G. S. Pleistocene Megafaunal collapse, novel plant communities, and enhanced fire regimes in North America. Science 326, 1100–1103 (2009).

  4. 4.

    Bakker, E. S. et al. Combining paleo-data and modern exclosure experiments to assess the impact of megafauna extinctions on woody vegetation. Proc. Natl Acad. Sci. USA 113, 847–855 (2016).

  5. 5.

    van der Putten, W. H., Macel, M. & Visser, M. E. Predicting species distribution and abundance responses to climate change: why it is essential to include biotic interactions across trophic levels. Philos. Trans. R. Soc. B 365, 2025–2034 (2010).

  6. 6.

    Jackson, S. T. Vegetation, environment, and time: the origination and termination of ecosystems. J. Veg. Sci. 17, 549–557 (2006).

  7. 7.

    Bradshaw, R. H. W. in Encyclopedia of Quaternary Science 2nd edn (ed Elias, S. A.) 699–704 (Elsevier, Amsterdam, 2013).

  8. 8.

    Grimm, E. C. et al. in Encyclopedia of Quaternary Science 2nd edn (ed Elias, S. A.) 831–838 (Elsevier, Amsterdam, 2013).

  9. 9.

    Seppa. H. in Encyclopedia of Quaternary Science 2nd edn (ed Elias, S. A.) 794–804 (Elsevier, Amsterdam, 2013).

  10. 10.

    Bigelow, N. H., Zazula, G. D. & Atkinson, D. E. in Encyclopedia of Quaternary Science 2nd edn (ed Elias, S.A.) 746–759 (Elsevier, Amsterdam, 2013).

  11. 11.

    Willerslev, E. et al. Diverse plant and animal genetic records from Holocene and Pleistocene sediments. Science 300, 791–795 (2003).

  12. 12.

    Willerslev, E. et al. Fifty thousand years of Arctic vegetation and megafaunal diet. Nature 506, 47–51 (2014).

  13. 13.

    Sønstebø, J. H. et al. Using next-generation sequencing for molecular reconstruction of past Arctic vegetation and climate. Mol. Ecol. Resour. 10, 1009–1018 (2010).

  14. 14.

    Yoccoz, N. G. et al. DNA from soil mirrors plant taxonomic and growth form diversity. Mol. Ecol. 21, 3647–3655 (2012).

  15. 15.

    Lozhkin, A.V. & Anderson, P.M. in Encyclopedia of Quaternary Science 2nd edn (ed Elias, S. A.) 27–38 (Elsevier, Amsterdam, 2013)

  16. 16.

    Anderson, P. M., Edwards, M. E. & Brubaker, L. B. in The Quaternary Period in the United States (eds Gillespie, A. R., Porter, S. C. & Atwater, B. F.) 427–440 (Elsevier, Amsterdam, 2004)

  17. 17.

    Lozhkin, A. V. & Anderson, P. M. Forest or no forest: implications of the vegetation record for climatic stability in Western Beringia during oxygen isotope stage 3. Quat. Res. 30, 2160–2181 (2011).

  18. 18.

    Miller, G. H. et al. Temperature and precipitation history of the Arctic. Quat. Sci. Rev. 29, 1679–1715 (2010).

  19. 19.

    Smith, S.E. & Read, D. J. Mycorrhizal Symbiosis 3rd edn (Academic Press, Amsterdam, 2008).

  20. 20.

    Brundrett, M. C. Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant Soil 320, 37–77 (2009).

  21. 21.

    Dickie, I. A., Koele, N., Blum, J. D., Gleason, J. D. & McGlone, M. S. Mycorrhizas in changing ecosystems. Botany 92, 149–160 (2014).

  22. 22.

    Wilkinson, D. M. Mycorrhizal fungi and quaternary plant migrations. Glob. Ecol. Biogeogr. Lett. 7, 137–140 (1998).

  23. 23.

    Elias, S. A. The problem of conifer species migration lag in the Pacific Northwest region since the last glaciation. Quat. Sci. Rev. 77, 55–69 (2013).

  24. 24.

    Moora, M. Mycorrhizal traits and plant communities: perspectives for integration. J. Veg. Sci. 25, 1126–1132 (2014).

  25. 25.

    Bueno, C. G. et al. Plant mycorrhizal status, but not type, shifts with latitude and elevation in Europe. Glob. Ecol. Biogeogr. 26, 690–699 (2017).

  26. 26.

    Hempel, S. et al. Mycorrhizas in the Central European flora: relationships with plant life history traits and ecology. Ecology 94, 1389–1399 (2013).

  27. 27.

    Terrer, C., Vicca, S., Hungate, B. A., Phillips, R. P. & Prentice, I. C. Mycorrhizal association as a primary control of the CO2 fertilization effect. Science 353, 72–74 (2016).

  28. 28.

    Kuyper, T. W. & De Goede, R. G. M. in Vegetation Ecology (eds Van der Maarel, E. & Franklin, J.) 260–284 (Blackwell, Chichester, 2013)

  29. 29.

    Vitousek, P. M., Menge, D. N. L., Reed, S. C. & Cleveland, C. C. Biological nitrogen fixation: rates, patterns and ecological controls in terrestrial ecosystems. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 368, 20130119 (2013)

  30. 30.

    St. John, M. G. et al. Loss of a dominant nitrogen-fixing shrub in primary succession: consequences for plant and below-ground communities. J. Ecol. 100, 1074–1084 (2012).

  31. 31.

    Gulbranson, E. L. et al. Nitrogen-fixing symbiosis inferred from stable isotope analysis of fossil tree rings from the Oligocene of Ethiopia. Geology 45, 687–690 (2017).

  32. 32.

    Menge, D. N. L. et al. Nitrogen-fixing tree abundance in higher-latitude North America is not constrained by diversity. Ecol. Lett. 20, 842–851 (2017).

  33. 33.

    Ollerton, J., Winfree, R. & Tarrant, S. How many flowering plants are pollinated by animals? Oikos 120, 321–326 (2011).

  34. 34.

    Kühn, I., Bierman, S. M., Durka, W. & Klotz, S. Relating geographical variation in pollination types to environmental and spatial factors using novel statistical methods. New Phytol. 172, 127–139 (2006).

  35. 35.

    Hoiss, B., Gaviria, J., Leingartner, A., Krauss, J. & Steffan-Dewenter, I. Combined effects of climate and management on plant diversity and pollination type in alpine grasslands. Divers. Distrib. 19, 386–395 (2013).

  36. 36.

    Dalsgaard, B. et al. Historical climate-change influences modularity and nestedness of pollination networks. Ecography 36, 1331–1340 (2013).

  37. 37.

    Williams, J. J. & Jackson, S. T. Novel climates, no-analog communities, and ecological surprises. Front. Ecol. Environ. 5, 475–482 (2007).

  38. 38.

    Hais, M., Komprdová, K., Ermakov, N. & Chytrý, M. Modelling the last glacial maximum environments for a refugium of Pleistocene biota in the Russian Altai Mountains, Siberia. Palaeogeogr. Palaeoclimatol. Palaeoecol. 438, 135–145 (2015).

  39. 39.

    Doledec, S., Chessel, D. & Gimaret-Carpentier, C. Niche separation in community analysis: a new method. Ecology 81, 2914–2927 (2000).

  40. 40.

    Murton, J. B. et al. Palaeoenvironmental Interpretation of Yedoma Silt (Ice Complex) Deposition as Cold-Climate Loess, Duvanny Yar, Northeast Siberia. Permafr. Periglac. Process. 26, 208–288 (2015).

  41. 41.

    Kaplan, J. O. et al. Climate change and Arctic ecosystems: 2. Modeling, paleodata-model comparisons, and future projections. J. Geophys. Res. Atmos. 108, 8171 (2003).

  42. 42.

    Gill, J. L. Ecological impacts of the late quaternary megaherbivore extinctions. New Phytol. 201, 1163–1169 (2014).

  43. 43.

    Binney, H. A. et al. Vegetation of Eurasia from the last glacial maximum to present: key biogeographic patterns. Quat. Sci. Rev. 157, 80–97 (2017).

  44. 44.

    Kytoviita, M. M. Asymmetric symbiont adaptation to Arctic conditions could explain why high Arctic plants are non-mycorrhizal. FEMS Microbiol. Ecol. 53, 27–32 (2005).

  45. 45.

    Shuman, B. & Finney, B. in Encyclopedia of Quaternary Sciences (ed Elias, S. A.) 1374–1383 (Elsevier, Amsterdam, 2006)

  46. 46.

    Clark, P. U. et al. Global climate evolution during the last deglaciation. Proc. Natl Acad. Sci. USA 109, E1134–E1142 (2012).

  47. 47.

    Schmitt, J. et al. Carbon Isotope constraints on the deglacial CO2 rise from ice cores. Science 336, 711–714 (2012).

  48. 48.

    Shaver, G. R. & Chapin, F. S. Response to fertilization by various plant growth forms in an Alaskan tundra – nutrient accumulation and growth. Ecology 61, 662–675 (1980).

  49. 49.

    Aerts, R., Callaghan, T. V., Dorrepaal, E., van Logtestijn, R. S. P. & Cornelissen, J. H. C. Seasonal climate manipulations have only minor effects on litter decomposition rates and N dynamics but strong effects on litter P dynamics of sub-arctic bog species. Oecologia 170, 809–819 (2012).

  50. 50.

    Beermann, F., Teltewskoi, A., Fiencke, C., Pfeiffer, E. M. & Kutzbach, L. Stoichiometric analysis of nutrient availability (N, P, K) within soils of polygonal tundra. Biogeochemistry 122, 211–227 (2015).

  51. 51.

    Jones, M. C. & Wu, Z. Rapid deglacial and early Holocene expansion of peatlands in Alaska. PNAS 107, 7347–7352 (2010).

  52. 52.

    Chapin, F. S., Shaver, G. R., Giblin, A. E., Nadelhoffer, K. J. & Laundre, J. A. Responses of arctic tundra to experimental and observed changes in climate. Ecology 76, 694–711 (1995).

  53. 53.

    Sundqvist, M. K., Wardle, D. A., Vincent, A. & Giesler, R. Contrasting nitrogen and phosphorus dynamics across an elevational gradient for subarctic tundra heath and meadow vegetation. Plant Soil 383, 387–399 (2014).

  54. 54.

    McLauchlan, K. K., Williams, J. J., Craine, J. M. & Jeffers, E. S. Changes in global nitrogen cycling during the Holocene epoch. Nature 495, 352–355 (2013).

  55. 55.

    Schweiger, O. et al. Multiple stressors on biotic interactions: how climate change and alien species interact to affect pollination. Biol. Rev. 85, 777–795 (2010).

  56. 56.

    Moeller, D. A. et al. Global biogeography of mating system variation in seed plants. Ecol. Lett. 20, 375–384 (2017).

  57. 57.

    Jacobson, G. L. Jr., Webb, T. III & Grimm, E. C. in The Geology of North America, Voi. K-3, North America and Adjacent Oceans during the Last Deglaciation (eds Ruddiman, W. F., Wright, H. E. Jr) 277–288 (Geological Society of America, Boulder, CO, 1987).

  58. 58.

    Huntley, B. Rates of change in the European palynological record of the last 13000 years and their climatic interpretation. Clim. Dynam. 6, 185–191 (1992).

  59. 59.

    Hart, M. M., Reader, R. J. & Klironomos, J. N. Life-history strategies of arbuscular mycorrhizal fungi in relation to their successional dynamics. Mycologia 93, 1186–1194 (2001).

  60. 60.

    Zobel, M. & Öpik, M. Plant and arbuscular mycorrhizal fungal (AMF) communities - which drives which? J. Veg. Sci. 25, 1133–1140 (2014).

  61. 61.

    de Bello, F. et al. On the need for phylogenetic ‘corrections’ in functional trait-based approaches. Folia Geobot. 50, 349–357 (2015).

  62. 62.

    Guisan, A. & Rahbek, C. SESAM - a new framework integrating macroecological and species distribution models for predicting spatio-temporal patterns of species assemblages. J. Biogeogr. 38, 1433–1444 (2011).

  63. 63.

    Lisiecki, L. E. & Raymo, M. E. A Pliocene-Pleistocene stack of 57 globally distributed benthic δ18O records. Paleoceanography 20, PA1003 (2005).

  64. 64.

    Guthrie, R.D. Frozen Fauna of the Mammoth Steppe (Chicago University Press, Chicago, 1990).

  65. 65.

    Boyer, F. et al. obitools: a unix-inspired software package for DNA metabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016).

  66. 66.

    Valentini, A., Pompanon, F. & Taberlet, P. DNA barcoding for ecologists. Trends Ecol. Evol. 24, 110–117 (2009).

  67. 67.

    Klotz, S., Kühn, I. & Durka, W. BIOLFLOR - Eine Datenbank mit Biologisch-Ökologischen Merkmalen zur Flora von Deutschland (Bundesamt für Naturschutz, Bonn, 2002)

  68. 68.

    Akhmetzhanova, A. A. et al. A rediscovered treasure: mycorrhizal intensity database for 3000 vascular plant species across the former Soviet Union. Ecology 93, 689–690 (2012).

  69. 69.

    Wang, B. & Qiu, L. Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza 16, 299–363 (2006).

  70. 70.

    Harley, J. L. & Harley, E. L. A check-list of mycorrhiza in the British flora. New Phytol. 105, 1–102 (1987).

  71. 71.

    Murray, D. F. in Differentiation Patterns in Higher Plants (ed. Urbanska, K. M.) 239–262 (Academic Press, London, 1987).

  72. 72.

    Brochmann, C. & Steen, S. W. Sex and genes in the flora of Svalbard – implications for conservation biology and climate change. Norske Vidensk. Akad. Mat. Nat. Kl. Skr. Ny. Ser. 38, 33–72 (1999).

  73. 73.

    Garnier, E. et al. Plant functional markers capture ecosystem properties during secondary succession. Ecology 85, 2630–2637 (2004).

  74. 74.

    Oksanen, J. et al. vegan: community ecology package. R package version 2.3-5. https://CRAN.R-project.org/package=vegan (2016)

  75. 75.

    Kleyer, M. et al. Assessing species and community functional responses to environmental gradients: which multivariate methods? J. Veg. Sci. 23, 805–821 (2012).

  76. 76.

    Legendre, P. Spatial autocorrelation: trouble or new paradigm? Ecology 74, 1659–1673 (1993).

  77. 77.

    Wagner, H. H. Direct multi-scale ordination with canonical correspondence analysis. Ecology 85, 342–351 (2004).

  78. 78.

    Durka, W. & Michalski, S. G. Daphne: a dated phylogeny of a large European flora for phylogenetically informed ecological analyses. Ecology 93, 2297–2297 (2012).

  79. 79.

    Revell, L. J. Phylogenetic signal and linear regression on species data. Methods Ecol. Evol. 1, 319–329 (2010).

Download references

Acknowledgements

We are grateful to F. de Bello for his constructive comments on the manuscript. The data production was supported by the European Union 6th framework project ECOCHANGE (GOCE-2006-036866, coordinated by P.T.). M.Z., J.D. and M.M. were supported by grants from the Estonian Research Council (IUT 20-28) and the European Regional Development Fund (Centre of Excellence EcolChange). C.B. was supported by the Research Council of Norway (grant no 191627/V40).

Author information

Affiliations

  1. Department of Botany, Institute of Ecology and Earth Sciences, University of Tartu, 40 Lai Street, 51005, Tartu, Estonia

    • Martin Zobel
    • , John Davison
    •  & Mari Moora
  2. Geography and Environment, University of Southampton, Highfield, Southampton, SO17 1BJ, UK

    • Mary E. Edwards
  3. Natural History Museum, University of Oslo, PO Box 1172 Blindern, NO-0318, Oslo, Norway

    • Christian Brochmann
  4. CNRS, Laboratoire d’Ecologie Alpine (LECA), Univ. Grenoble Alpes, F-38000, Grenoble, France

    • Eric Coissac
    •  & Pierre Taberlet
  5. Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark

    • Eske Willerslev
  6. Department of Zoology, University of Cambridge, Downing St, Cambridge, CB2 3EJ, UK

    • Eske Willerslev

Authors

  1. Search for Martin Zobel in:

  2. Search for John Davison in:

  3. Search for Mary E. Edwards in:

  4. Search for Christian Brochmann in:

  5. Search for Eric Coissac in:

  6. Search for Pierre Taberlet in:

  7. Search for Eske Willerslev in:

  8. Search for Mari Moora in:

Contributions

M.M. and M.Z. designed the study. M.M., J.D. and C.B. compiled the trait database and J.D. performed the analyses of the data. M.Z., J.D., M.E. and M.M. wrote the first draft of the paper, C.B., P.T., E.W. and E.C. discussed the results and contributed to the writing of the final manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Mari Moora.

Electronic supplementary material

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Creative Commons BY

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.