The deep biosphere is one of the least understood ecosystems on Earth. Although most microbiological studies in this system have focused on prokaryotes and neglected microeukaryotes, recent discoveries have revealed existence of fossil and active fungi in marine sediments and sub-seafloor basalts, with proposed importance for the subsurface energy cycle. However, studies of fungi in deep continental crystalline rocks are surprisingly few. Consequently, the characteristics and processes of fungi and fungus-prokaryote interactions in this vast environment remain enigmatic. Here we report the first findings of partly organically preserved and partly mineralized fungi at great depth in fractured crystalline rock (−740 m). Based on environmental parameters and mineralogy the fungi are interpreted as anaerobic. Synchrotron-based techniques and stable isotope microanalysis confirm a coupling between the fungi and sulfate reducing bacteria. The cryptoendolithic fungi have significantly weathered neighboring zeolite crystals and thus have implications for storage of toxic wastes using zeolite barriers.
The deep subsurface biosphere comprises microorganisms several kilometers below the surface1. Microbiological investigations have revealed active deep ecosystems in marine sediments2, deep-sea hydrothermal vents3, sub-seafloor igneous rocks4, and terrestrial sedimentary5 and crystalline rocks6. Owing to its recent discovery and the difficulties in accessing samples, the deep biosphere is among the least understood ecosystems on Earth. Although processes are relatively slow because of the low energy supply7 the deep ecosystems are proposed to comprise a significant biomass and play an important role in the energy cycling of the Earth8. Estimates from the deep continental subsurface suggest that this environment accommodates a significant part of the Earth’s biomass (up to 19%)8. Until recently, the majority of the microbiological investigations in the deep biosphere have been focused on prokaryotes, and the potential presence of eukaryotes such as fungi has been largely neglected9, 10. Recently, fungi have been found to exist and have been isolated from various deep marine settings10,11,12,13,14 including sub-seafloor basalt9, 15,16,17, suggesting that fungi play a major role in the element and energy cycling9. In continental deep settings studies involving fungi are surprisingly rare. Reitner et al.18 described fossilized putative fungal hyphae in the Triberg granite, Germany, and Ivarsson et al.19 described fossilized mycelia from the Lockne impact structure, Sweden, of Ordovician age. Ekendahl et al.20 isolated a few strains of yeast fungi from fluids at Äspö, Sweden, and Sohlberg et al.21 examined the total fungal diversity in anaerobic bedrock fractures at Olkiluoto, Finland, and found the diversity to be higher than expected consisting of most major fungal phyla, some minor phyla and even novel species. At great depth in continental granite aquifer systems anoxic conditions prevail, and the fungi living there are considered anaerobic21. Anaerobic fungi are so far poorly understood in an environmental context, reported only from a few anoxic settings14, 22, but are most thoroughly described from rumina of ruminating herbivores23, 24. Because of the production of H2 during their respiration, anaerobic fungi consort with H2-dependent methanogenic and acetogenic archaea in the rumen, which enhances growth of both organisms. Potentially any H2-dependent chemoautotrophic microorganism could be fuelled by anaerobic fungi in an anoxic environment25 and it has been suggested that anaerobic fungi represent a neglected geobiological force in the subsurface ecosystem9. Direct evidence of such consortia in the subsurface remains to be confirmed. However, the fungi that form symbiotic relationships with acetogens and methanogens in the rumen have recently been described from marine sediments26. Even though the presence of fungi in the continental crystalline basement is confirmed by a few reports, there is a huge gap in knowledge compared to what is known about the prokaryotes, and there is an urgent need to investigate the abundance, diversity and ecological role of fungi in these deep environments.
Here we present an extensive study of previously unseen organically preserved and partly mineralized fungal hyphae, inferred as anaerobic fungi, from deep fractured continental crystalline rocks (740 m depth at the Laxemar site in Sweden). Utilization of state-of-the-art methodology including secondary ion mass spectrometry (SIMS), synchrotron radiation X-ray tomographic microscopy (SRXTM), and Time-of-Flight (ToF-) SIMS enables comprehensive new characterisation of the fungi, the cryptoendolithic behaviour of fungi, and the prokaryote-fungus interaction in this environment. This not only increases the understanding of the role fungi play in the energy cycling of the deep biosphere, but also has societal implication for long-term storage of toxic wastes.
Mycelial-like networks consisting of long filamentous structures were discovered at 740 m depth in a drill core (KLX09) at the Laxemar site, Sweden, where the Swedish Nuclear Fuel and Waste Management Co. (SKB) recently carried out site investigations for a deep nuclear-waste repository. At this site evidence of both active27, 28 and ancient prokaryotic activity, such as bacterial sulfate reduction29 and anaerobic oxidation of methane30, has been reported. The mycelial-like networks occur in a partly open fracture in a meter-wide quartz vein (Supplementary Fig. 1). The fracture walls are coated by euhedral zeolite, identified as cowlesite by Raman spectroscopic analysis, as well as calcite, pyrite and clay minerals. The filaments cover an area of about 25–30 cm2 of the fracture walls in the drill core sample. The filaments are undulating and typically have a diameter of 5–20 µm (Figs 1a and 2), consistent throughout the filaments, while the length of the filaments extends up to several hundred micrometres in length. The filaments branch frequently, forming both Y- and T-junctions and occasional anastomoses between branches (Fig. 1b–d, f). The overall architecture of the networks is an intertwined, mycelium-like structure where the filaments are interconnected (Fig. 1c). The filaments are characterized by a central strand encircled by a thicker marginal wall. At places where filaments have been broken off, probably during sampling or preparation, the central strand is clearly visible in cross-section occupying about a third of the total filament diameter (Fig. 1e).
The filaments are either mineralized or carbonaceous in composition. Energy Dispersive X-ray Analysis (EDX) and Raman spectroscopic analysis show that the mineralized parts of the fossilized filaments are dominated by Fe/Mg/Ca-rich clay minerals, together with some minor Fe-oxide. The central strand is composed of Fe-oxides and surrounded by the clay phase. EDX analyses indicate the presence of carbon throughout the filaments, even where these are mineralized.
Raman spectroscopic analysis on the mineralized filaments and the carbonaceous filaments shows characteristics of typical organic compounds with three clear peaks at 1300, 1440 and 1660 cm−1, which may be attributed to CH2 and CH3 deformations and C=C stretching vibrations similar to what has been found in fatty acids31. The spectra further show several bands between 2720 and 3300 cm−1 with the most intense at 2850 and 2930 cm−1 assigned to CH2 and CH3 stretching vibrations.
There are detectable biomarkers in the carbonaceous filaments, but none of them are specific for prokaryotes or eukaryotes. The gas chromatography (GC)–MS analyses mainly detected fatty acids and aromatic substances like dimethylnaphthalenes, trimethylnaphthalenes, and methylbiphenyls (Fig. 3 and Supplementary Table 1), which are characteristic for thermal maturation. The Time-of-Flight (ToF)-SIMS results of partly mineralized hyphae show possible association of sulfur (pyrite), SO4H− and PO2 with sugar fragments (potentially chitin-related fragment as the C3H3O2 ion produces a strong signal in the chitin standard spectrum, but also in spectra of other types of sugar molecules), and fatty acids (C16:0 and C18:1 at m/z 255.23 and 281.25, respectively, Supplementary Fig. 2). The mineralized filaments and carbonaceous filaments occur side by side as parts of the same mycelium but at various stages of fossilization (Fig. 1a). There are also previously unseen gradual transitions from carbonaceous parts to mineralized parts in single filaments (Fig. 4). In addition to the filaments, there is a carbonaceous/partly mineralized biofilm, a few micrometres thick, that covers parts of the minerals and from which some filaments, especially the carbonaceous ones, originate (Fig. 2b).
Significant weathering has occurred at the contact between the mycelium-forming microorganism (including the biofilm) and the secondary mineralizations (zeolite and calcite, Fig. 2). Such weathering is absent in minerals not covered by the mycelium. The minerals have a rough and clearly etched surface when associated with the mycelium. Filaments creeping along a mineral surface leave an etched gorge-like channel in the surfaces (Fig. 2c, d). Filaments are also continually penetrating the minerals through micro-fractures as revealed by the SRXTM investigations (Fig. 2e).
Pyrite crystals are widely distributed out in the fracture void (Fig. 5a), in particular in association with hyphae, and occur as two different generations. The older pyrite generation consists of cubic and octahedral crystals ranging between 30 and 70 µm (Fig. 6a), and the younger more fine-grained pyrite (2–25 µm) occurs partly as overgrowths on the older pyrite generation (Fig. 6a) but mainly as fine-grained cubic crystals on the mycelial-forming filaments (Figs 5b–e and 6b). Detailed micro-scale analyses (n = 56) of S-isotopes show that the older pyrite generation ranges in δ34S from +12.3 to +30.4‰ V-CDT (denoting Vienna Canyon Diablo Troilite), with most values in a quite narrow span around +23 ± 4‰ (Fig. 6c). The younger pyrite generation has δ34S values relatively evenly distributed from −53.3 to +0.3‰ (Figs. 6c, d and Supplementary Table 2).
Detailed micro-scale isotope analyses were also carried out on calcite (n = 47 for C-isotopes and n = 44 for O-isotopes). The variability of δ13C was 42.7‰ V-PDB, (denoting Vienna Pee Dee Belemnite) and of δ18O 16.5‰ V-PDB (Fig. 7c; Supplementary Table 3). The calcite crystals show distinct growth zonation that represents at least three precipitation events (generations) with specific stable isotope compositions. Analytical transects within the crystals show that the oldest generation has highest δ13C and lowest δ18O (Fig. 7a, d, zoned crystal with growth direction from left to right). This is followed by a generation strongly depleted in 13C (−43 to −26‰) and with highest δ18O (−11 to −4.4‰), whereas the youngest generation with δ13C of −24 to −16‰ and δ18O of −15 to −11‰ (Fig. 7b, e) is most affected by microorganism-related chemical weathering. Single phase liquid fluid inclusions in the calcite (Supplementary Fig. 3) indicate formation temperatures below 50 °C32. The inclusions show low eutectic melting temperature close to −50 °C, which points to a composition where CaCl2 is the most abundant salt. Final ice melting temperatures in the range of −22.4 to −23.5 °C correspond to salinities of 21.7–22.2 wt.% CaCl2.
The mycelium-like appearance, the diameter, and the anastomosing behavior of the filaments (Fig. 1) are all distinctive features of fungi. The presence of a mineralized central strand has been shown as a common feature among fossilized fungal hyphae33,34,35,36. Lining of mineral surfaces by a basal biofilm from which further hyphal growth emanates to form a mycelium is also typical for endolithic fungi15, 19, 33, 34. Except for fungi, actinobacteria and the stramenopile oomycetes are the only microorganisms forming mycelium-like networks of branching filaments. However, actinobacterial filaments never exceed 2 µm in diameter and anastomoses have not been confirmed37, 38. Thus, the diameter of the current filaments, together with the presence of anastomoses excludes an actinobacterial interpretation. Oomycete filaments have been reported to form anastomoses, but as a means of conjugation rather than as a structural feature39. Based on these morphological features we infer a fungal interpretation of the networks and suggest that they represent diagenetically mineralized fungal hyphae. As further support for this, the weathered mineral surfaces in contact with the fungi bear close resemblance to fungal induced weathering seen in many other minerals, owing to production of organic acids40. Active boring seen among fungi in sub-seafloor basalts is not observed in the sample of the present study. However, the fungal hyphae typically exploit microfractures in the minerals for penetration.
The partly mineralized nature is in itself a rare finding, which has previously only been observed in the laboratory41, 42. Usually, subsurface fungi are completely fossilized to clay minerals and Fe-oxides with rare carbonaceous elements and no biomarkers at all33, 34, except rare chitin observations16. Our findings give insights into the fossilization process of fungi through a transition from maturation of the organic matter to a carbonaceous material, before being finally mineralized by clays and Fe-oxides. Based on our observations the mineralization starts from the centre of the hypha with a fully mineralized Fe-oxide dominated central strand and clay-mineral dominated margins. The negatively charged carbonaceous material attracts the positively charged Si, Al, Mg, Fe (and minor Na, and Ca) cations of the clays. Initial adsorption of cations on the carbonaceous hyphae sparks subsequent adsorption and clay mineralization34, 43. The end result of complete mineralization by clays and Fe-oxides is in agreement with previous observations of deep fossilized fungi and supports what seems to be an overall characteristic pattern of fungal fossilization in deep ecosystems.
Fungi are heterotrophs and need access to carbohydrates like mono- or polysaccharides for their metabolism. In the deep oligotrophic granite environment, the most likely source of carbohydrates is living or dead bacterial biofilms44. The large 13C-depletions of the calcite, resulting in δ13C values as low as −43‰ (Fig. 7c), point to oxidation of methane in the fracture system45, as described previously for this setting30. The youngest generation of calcite shows δ13C values suggesting microbial degradation of organic C. Remnant biofilms of metanotrophs, as well as sulfate-reducing bacteria (SRB) that evidently have occupied the fracture at several occasions, may have acted as nutrients for the fungi and triggered the fungal colonization of the system.
The fungi cannot be taxonomically classified in detail because of the fossilization and lack of morphological features like septa, but they are considered to have grown in an anaerobic environment. A major support for this is that the current groundwater in the fracture network turns anoxic in the upper tens of meters46 and below that depth remains strictly reducing, with Eh values in the range of −300 to −200 mV47. Based on these features, it seems highly unlikely that the conditions in the paleo-groundwater would have been oxidising or suboxic at a depth as great as 740 m. There are several additional lines of support for prolonged anoxic conditions. First, there is pyrite in relation to the hyphae; second, oxidation-related alteration features are not detected in any of the pyrite generations in the fractures, in contrast to pyrite at shallower depth where waters with dissolved oxygen have infiltrated46; third, Ce(IV) and positive Ce anomalies, which are indicative of oxidising conditions, are frequent in fracture coatings in the upper 10 m of the bedrock but absent below that depth48; and, fourth, abundant signs of anaerobic oxidation of methane (13C-depleted calcite, in this fracture and elsewhere in the fracture network30). There is thus strong evidence that the fungi were anaerobic, in a manner similar to fungi filtered from deep-water samples from fractured crystalline rocks in Finland21. The partly mineralized nature of the fungi and the degraded and matured carbon in the fungi speak against a modern origin. The only timing indication available is offered by the fluid inclusions in the calcite showing <50 °C, which rule out formation prior to the Mesozoic era based on the uplift history of the area49, 50, but it should be emphasized that this is a maximum age estimate and not a direct age determination. In addition, the calcite crystals show fluid inclusion salinities that are much higher than in the present groundwater.
The S−-isotope signatures indicate that the older pyrite generation precipitated from a more homogeneous fluid than the fine-grained younger generation of pyrite (Fig. 6). The relatively small variation in δ34S of the older pyrite generation likely reflects formation during bacterial sulfate reduction (BSR) at relatively open system conditions. The younger fine-grained pyrite has a more clear relation to the hyphae than the older pyrite, and, hence, the discussion about the hyphae-SRB relation only takes the younger of these two pyrite generations into account. These fine-grained pyrite crystals were produced via the activity of SRB, because the low δ34S values,are diagnostic for microbial transformation of sulfate to sulfide as 32SSO4 is favoured over 34SSO4 in the SRB metabolism51. The large span in δ34S of this pyrite generation is interpreted as the result of Rayleigh type distillation where the δ34S composition of the sulfate and thus of the precipitated pyrite became progressively higher as the sulfate pool was exhausted during BSR under closed system conditions51. Coexistence of the SRB producing these pyrite crystals and the fungi is thus possible and supported by a number of features. First, pyrite grows on original hyphal walls and not on parts that have been exposed by later breakdown/degradation caused by the core drilling or sample preparation, and clusters of pyrite enfold hyphae, forming almost a girdle-like structure that follows the hyphal morphology tightly (Fig. 5b, c); second, lack of hyphal degradation at the direct contact with the pyrite and no sign of pervasive replacement of hypha by pyrite, which is the common case in complete pyritization of fossils caused by heterotrophic bacterial activity52, 53, argue against a situation where SRB only scavenged the fungal biomass; third, hyphal growth has been influenced by the presence of pyrite crystals; for example, hyphae have grown around existing pyrite that sits upon other hyphae (Fig. 5c) and pyrite crystals are partly enclosed by the hyphae (Fig. 5d, e); and fourth, spatial relation between pyrite, fatty acids and sugar compounds that resemble chitin, as revealed by Tof-SIMS analyses.
Anaerobic fungal species have no mitochondria and are unable to produce energy by either aerobic or anaerobic respiration54, 55. Instead, anaerobic fungi have hydrogenosomes, and produce mainly H2, but also formate, lactate, acetate and carbon dioxide, as metabolic waste products54, 56. Anaerobic fungi consort with H2-dependent methanogenic archaea in the rumen of ruminants, but potentially any H2-dependent chemoautotrophic microorganism could be fuelled by anaerobic fungi in an anoxic environment25, for instance SRB. Although the largest S-isotope fractionations observed in pure culture experiments have been associated with heterotrophic BSR57, autotrophic BSR using H2 also involves significant S-isotope fractionation (δ34SH2S-δ34SSO4 of up to 37‰), particularly at low H2 concentrations and slow BSR rates58. Because the in situ rate of bacterial processes and generally also the concentrations of H2 appear to be substantially lower in the granitic fractures6, 59 than those manipulated in the laboratory, larger fractionation than reported from the laboratory appears reasonable under the extreme oligotrophy in the deep granite fractures. Hence, H2 is a plausible electron donor for the SRB that produced the younger generation of pyrite, in line with the fact that the current groundwater at the site carries autotrophic microorganisms alongside heterotrophic ones27. We accordingly propose that H2, and potentially some other substrate such as acetate, provided by anaerobic fungi, have triggered SRB growth (Fig. 8) and that, consequently, the intimate relationship between the fungal mycelium and the pyrite crystals represents a fossilized consortium of anaerobic fungi and SRB being the first record of these previously hypothesized communities25. This further suggests that the deep oligotrophic biosphere in crystalline continental rocks may be a neglected vast fungal habitat.
Hydrogen gas has been proposed to be an important substrate for deep subsurface lithoautotrophic ecosystems60,61,62,63 and a limiting factor for the persistence of an indigenous SRB community64, 65. However, the formation and origin of H2 remain elusive, and several different processes have been proposed, including radiolysis60 over long time spans in the subsurface environment. Investigations from the Fennoscandian shield show highly variable H2 concentrations in the deep groundwater (down to 1000 m depth) with concentrations up to 190 µl/l27, 59, that are correlated with neither depth nor residence times of the waters, which in several cases are in the order of just a couple of thousand years66. Theseare certainly too short time periods for build-up of significant H2 concentrations by radiolysis (cf. ref. 60) implying that radiolysis is not the sole source of the elevated H2 concentrations. Instead, based on our findings and ambiguous traces of fungi in the deep aquifer at Olkiluoto, Finland21, we propose that subsurface fungi are neglected and likely significant providers of H2 for autotrophic microbial processes in the oligotrophic crystalline continental crust.
Anaerobic fungi could potentially pose an environmental threat to barriers in geological repositories of toxic wastes, via two mechanisms: mediating direct bioerosion of the barrier system by chemical dissolution, as well as supporting an H2-dependent SRB community capable of causing corrosion to copper canisters containing spent nuclear fuel67. Regarding the first mechanism, the extensive weathering of zeolites seen in the granite fracture in the present study, as well as similar observations in sub-seafloor basalts elsewhere33, 34, calls for consideration when planning to use this group of minerals as geochemical barriers in subsurface storages. Zeolites have been planned to function as an ion-exchange retention barrier for the storage of high-level nuclear waste in the US68, 69. The cryptoendolithic behavior of anaerobic fungi may challenge the long-term stability of such systems, at least at low-temperatures. Regarding the second mechanism, our study shows a previously unseen relation between fungi and SRB at great depth in fractured granite which may, alongside long-term radiolytic H2 consumption coupled to sulfate reduction, enhance sulfide levels in the deep groundwater aquifer. The ultimate result may be higher rates of sulfide-induced copper corrosion and CuS formation64, 65. The recognition of fungi in the subsurface realm, thus, indicates the presence of a previously neglected geobiological agent, the environmental impact and societal implications of which have yet to be accounted for.
Following sample characterisation in situ on the fracture surfaces using a Hitachi S-3400N scanning electron microscope (SEM) equipped with an integrated energy-dispersive spectroscopy (EDS) system under low-vacuum conditions, calcite and pyrite crystals were mounted in epoxy, polished to expose crystal cross-sections and examined again using SEM (to trace zonations). Intra-crystal SIMS-analysis of carbon, oxygen and sulfur isotopes were performed on a Cameca IMS1280 ion microprobe, at the NordSIM facility at the Museum of Natural History, Stockholm, Sweden. Analytical transects were made within the crystals. In total 47 analyses were made for δ13C and 44 for δ18O in calcite and 55 for δ34S of pyrite in samples with AOM-signature in the calcite. Set up follows descriptions in ref. 30. Sulfur was sputtered using a 133Cs+ primary beam with 20 kV incident energy (10 kV primary, −10 kV secondary) and a primary beam current of ~ 1.5 nA, producing secondary ions from a slightly elliptical area of ~ 10 μm (long axis, depth dimension is 1–2 μm). A normal incidence electron gun was used for charge compensation. Analyses were performed in automated sequences, with each analysis comprising a 70 s presputter to remove the gold coating over a rastered 25 × 25 µm area, centring of the secondary beam in the field aperture to correct for small variations in surface relief, and data acquisition in 16 4-s integration cycles. The magnetic field was locked at the beginning of the session using an NMR field sensor. Secondary ion signals for 32S and 34S were detected simultaneously using two Faraday detectors with a common mass resolution of 4860 (M/ΔM). O was measured on two Faraday cages (FC) at mass resolution 2500, whereas C used a FC/EM combination, with mass resolution 2500 on the 12C peak and 4000 on the 13C peak to resolve it from 12C1H. Data were normalized for instrumental mass fractionation using matrix matched standards which were mounted together with the sample mounts and analyzed after every sixth sample analysis. Isotope data from calcite were normalized using calcite standard S0161 and the Ruttan standard was used for pyrite (recommended values provided in Supplementary Tables 2 and 3). Precision was δ18O: ± 0.2‰, δ13C: ± 0.4‰ and δ34S: ± 0.13‰. Significant influence of organic carbon was avoided in the SIMS-analyses by careful spot placement to areas in the crystals without micro-fractures or inclusions, at a sufficient distance from grain-boundaries where fine-grained clusters of other minerals and remnants of organic material may appear. The uncertainty associated with potential organic inclusions and matrix composition is therefore considered to be insignificant compared to the isotopic variations.
Right before ToF-SIMS analyses, the rock containing fractures with hyphae was cracked open, using clean tweezers (heptane, acetone and ethanol in that order), to expose fresh hyphae surfaces. The small pieces of rock containing the hyphae were then mounted with clean tweezers on double-sticky tape on a silica wafer. The ToF-SIMS analysis was performed on a ToF-SIMS IV (ION-TOF GmbH) by rastering a 25 keV Bi3 + beam (pulsed current of 0.1 pA) over an area of ~ 300 × 300 µm for 200–300 s. Analyses were performed in positive and negative mode at high mass resolution (bunched mode: Δl ~ 3 µm, m/Δm ~ 2000–4000 at m/z 30). As a control, additional spectra were also acquired from the tape to confirm that samples had not been contaminated by it.
The tomographic measurements were carried out on the TOMCAT beamline at the Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. A total of 1501 projections were acquired equiangularly over 180°, post-processed online and rearranged into flat- and darkfield-corrected sinograms. The beam energy used for the six aliquots was 25 (n = 1), 30 (3), 35 (1) or 40 (1) keV, for maximum absorption contrast. Specimens were scanned with a 10x objective, resulting in cubic voxel dimensions of 0.65 µm. Visualization was done using Avizo 9.1.1 (FEI Company).
Extraction of fungi and a sea sand blank reference sample for analyses of organic compounds was carried out accordingly. Powder (1–2 g) was extracted with 4 ml of dichloromethane/methanol (3:1) in a Teflon-capped glass vial (ultrasonication, 35 min, 50 °C). The supernatant was decanted after centrifuging. Extraction was repeated with dichlormethane and afterwards with n-hexane. After evaporation of the combined extracts and re-dissolution in pure dichloromethane, the solvents were dried with N2. Extracts were re-dissolved with 20 µl of n-Hexane and derivatized by adding 20 µl BSTFA/Pyridine and heated (40 °C, 1.5 h). Remnant powder was decarbonized with TMCS/Methanol (2:9) and derivatized by heating (80 °C, 1.5 h). The lipid fraction was separated by mixing with hexane and decanting the supernate. Extraction was repeated three times. The samples were dried with N2, redissolved with 1 ml of n-Hexane and analyzed with GC/MS. For the analysis of the kerogen fraction ca 35 mg of sample extraction residues were mixed with sea sand (glowed for 2 h at 550 °C) and Molybdenum—catalyst. Catalytic hydropyrolysis (HyPy) was conducted with a constant H2 flow at 5 l/min and a temperature program from 20 to 250 °C for 50 min and 250° to 500 °C for 8 min using a device from Strata Technology Ltd. (Nottingham, UK). The generated pyrolysate was absorbed on silica gel in the dry ice cooled trap tube. The HyPy pyrolysates were separated into an aliphatic, aromatic and polar fraction using column chromatography. To avoid any contamination, only pre-distilled solvents were used. All glassware used was first glowed at 500 °C. Solvent blank extracts (with pre-heated sea sand) were performed concomitantly as contamination controls and measured together with the investigated samples. One microlitre of each sample extract was analyzed with Thermo Trace 1310 GC coupled to a Thermo TSQ Quantum Ultra triple quadrupole MS. The GC was equipped with a fused silica capillary column (5 MS, 30 m lengths, 0.25 mm i.d., 0.1 µm film thickness, with He as carrier gas. The temperature program of the GC oven was 80 to 310 °C. The MS source was kept at 240 °C in electron impact mode at 25 eV ionization energy. Compounds and corresponding characteristic fragments detected with GC/MS are listed in Supplementary Table 1.
Analysis was performed following30 of fungi and for identification of minerals in a polished section using a Horiba instrument LabRAM HR 800 confocal laser Raman spectrometer equipped with a multichannel air-cooled CCD array detector. A low laser power 0.05 mW was used to avoid laser induced degradation of the sample. An Olympus BX41 microscope was coupled to the instrument. The laser beam was focused through a ×100 objective to obtain a spot size of about 1 µm. The accuracy of the instrument was controlled by repeated use of a silicon wafer calibration standard with a characteristic Raman line at 520.7 cm−1. The Raman spectra were achieved with LabSpec 5 software.
Fluid Inclusion Microthermometry
Fluid inclusions were analyzed in handpicked calcite crystals (0.5–1.5 mm in size). Microthermometric analysesof fluid inclusions were made with a Linkam THM 600 stage mounted on a Nikon microscope utilizing a ×40 long working-distance objective. The working range of the stage is from −196° to +600 °C. The thermocouple readings were calibrated by means of SynFlinc synthetic fluid inclusions and well-defined natural inclusions in Alpine quartz. The reproducibility was ± 0.1 °C for temperatures below 40 and ± 0.5 °C for temperatures above 40 °C.
All relevant data are included in the Supplementary material to this article.
Glud, R. N. et al. High rates of microbial carbon turnover in sediments in the deepest oceanic trench on Earth. Nat. Geosci. 6, 284–288 (2013).
Parkes, R. J. et al. Deep sub-seafloor prokaryotes stimulated at interfaces over geological time. Nature. 436, 390–394 (2005).
Jorgensen, B. B., Isaksen, M. F. & Jannasch, H. W. Bacterial sulfate reduction above 100 °C in deep-sea hydrothermal vent sediments. Science 258, 1756–1757 (1992).
Schrenk, M. O., Huber, J. A. & Edwards, K. J. Microbial provinces in the subseafloor. Annu. Rev. Mar. Sci. 2, 279–304 (2009).
Fredrickson, J. K. et al. Microbial community structure and biogeochemistry of Miocene subsurface sediments: implications for long-term microbial survival. Mol. Ecol. 4, 619–626 (1995).
Pedersen, K. et al. Numbers, biomass and cultivable diversity of microbial populations related to depth and borehole-specific conditions in groundwater from depths of 4 to 450 m in Olkiluoto, Finland. ISME J. 2, 760–775 (2008).
Wu, X. et al. Microbial metagenomes from three aquifers in the Fennoscandian shield terrestrial deep biosphere reveal metabolic partitioning among populations. ISME J. 10, 1192–1203 (2016).
McMahon, S. & Parnell, J. Weighing the deep continental biosphere. FEMS Microbiol. Ecol. 87, 113–120 (2014).
Ivarsson, M., Bengtsson, S. & Neubeck, A. The igneous oceanic crust-Earth’s largest fungal habitat. Fungal Ecol 20, 249–255 (2016).
Orsi, W. D., Biddle, J. R. & Edgcomb, V. D. Deep sequencing of subseafloor eukaryotic rRNA reveals active fungi across marine subsurface provinces. PLoS ONE. 8, 1–10 (2013).
Le Calvez, T., Burgaud, G., Mahé, S., Barbier, G. & Vandenkoornhuyse, P. Fungal diversity in deep-sea hydrothermal ecosystems. Appl. Environ. Microbiol. 75, 6415–6421 (2009).
López-García, P., Vereshchaka, A. & Moreira, D. Eukaryotic diversity associated with carbonates and fluid–seawater interface in Lost City hydrothermal field. Environ. Microbiol. 9, 546–554 (2007).
Connell, L., Barrett, A., Templeton, A. & Staudigel, H. Fungal diversity associated with an active deep sea volcano: Vailulu’u Seamount, Samoa. Geomicrobiol. J. 26, 597–605 (2009).
Nagano, Y. & Nagahama, T. Fungal diversity in deep-sea extreme environments. Fungal Ecol 5, 463–471 (2012).
Ivarsson, M. et al. Fossilized fungi in subseafloor Eocene basalts. Geology. 40, 163–166 (2012).
Ivarsson, M., Holm, N. G. & Neubeck, A. in Trace Metal Biogeochemistry and Ecology of Deep-Sea Hydrothermal Vent Systems (eds Demina L. L. & Galkin V. S.), 143-166 (Springer International Publishing, 2016).
Hirayama, H. et al. Data report: cultivation of microorganisms from basaltic rock and sediment cores from the North Pond on the western flank of the Mid-Atlantic Ridge, IODP Expedition 336. Proc. Int. Oc. Drill. Prog. 336, (2015). doi:10.2204/iodp.proc.336.204.2015.
Reitner, J., Schumann, G. & Pedersen, K. in Fungi in Biogeochemical Cycles (Cambridge Univ. Press, 2006).
Ivarsson, M. et al. Fungal colonization of an Ordovician impact-induced hydrothermal system. Sci. Rep. 3, 3487 (2013).
Ekendahl, S., O’Neill, H. A., Thomsson, E. & Pedersen, K. Characterisation of yeasts isolated from deep igneous rock aquifers of the fennoscandian shield. Microb. Ecol. 46, 416–428 (2003).
Sohlberg, E. et al. Revealing the unexplored fungal communities in deep groundwater of crystalline bedrock fracture zones in Olkiluoto, Finland. Front. Microbiol. 6, 573 (2015).
McDonald, J. E., Houghton, J. N. I., Rooks, D. J., Allison, H. E. & McCarthy, A. J. The microbial ecology of anaerobic cellulose degradation in municipal waste landfill sites: evidence of a role for fibrobacters. Environ. Microbiol. 14, 1077–1087 (2012).
Khejornsart, P. & Wanapat, M. Diversity of rumen anaerobic fungi and methanogenic archaea in swamp buffalo influenced by various diets. J. Anim. Vet. Adv. 9, 3062–3069 (2010).
Liggenstoffer, A. S., Youssef, N. H., Couger, M. B. & Elshahed, M. S. Phylogenetic diversity and community structure of anaerobic gut fungi (phylum Neocallimastigomycota) in ruminant and non-ruminant herbivores. ISME J. 4, 1225–1235 (2010).
Ivarsson, M., Schnürer, A., Bengtson, S. & Neubeck, A. Anaerobic fungi: a potential source of biological H2 in the oceanic crust. Front. Microbiol. 7, 674 (2016).
Picard, K. T. Coastal marine habitats harbor novel early-diverging fungal diversity. Fungal Ecol 25, 1–13 (2017).
Hallbeck, L. & Pedersen, K. Characterization of microbial processes in deep aquifers of the Fennoscandian Shield. Appl. Geochem. 23, 1796–1819 (2008).
Drake, H., Tullborg, E.-L., Sandberg, B., Blomfeldt, T. & Åström., M. E. Extreme fractionation and micro-scale variation of sulphur isotopes during bacterial sulphate reduction in deep groundwater systems. Geochim. Cosmochim. Acta. 161, 1–18 (2015).
Drake, H., Åström, M., Tullborg, E.-L., Whitehouse, M. J. & Fallick, A. E. Variability of sulphur isotope ratios in pyrite and dissolved sulphate in granitoid fractures down to 1 km depth-evidence for widespread activity of sulphur reducing bacteria. Geochim. Cosmochim. Acta. 102, 143–161 (2013).
Drake, H. et al. Extreme 13C-depletion of carbonates formed during oxidation of biogenic methane in fractured granite. Nat. Commun. 6, 7020 (2015).
De Gelder, J., De Gussern, K., Vandenabeele, P. & Moens, L. Reference database of Raman spectra of biological molecules. J. Raman Spectrosc. 38, 1133–1147 (2007).
Goldstein, R. H. Fluid inclusions in sedimentary and diagenetic systems. Lithosphere 55, 159–193 (2001).
Bengtson, S. et al. Deep-biosphere consortium of fungi and prokaryotes in Eocene subseafloor basalts. Geobiology 12, 489–496 (2014).
Ivarsson, M., Bengtson, S., Skogby, H. & Belivanova, V. Fungal colonies in open fractures of subseafloor basalt. Geomar. Lett. 33, 233–234 (2013).
Deacon, J. W. Modern Mycology (Wiley Blackwell, 1997).
Smith, S. E. & Read, O. J. Mycorrhiza symbiosis (Academic Press, 1997).
Erikson, D. The morphology, cytology, and taxonomy of the actinomycetes. Ann. Rev. Microbiol. 3, 23–54 (1949).
Higgins, M. L. & Silvey, J. K. G. Slide culture observations of two freshwater actinomycetes. Trans. Am. Microsc. Soc. 85, 390–398 (1966).
Stephenson, L. W., Erwin, D. C. & Leary, J. V. Hyphal anastomosis in Phytophthora capsici. Phytopathology. 64, 149–150 (1974).
Gadd, G. M. Geomycology: biogeochemical transformations of rocks, minerals, metals and radionuclides by fungi, bioweathering and bioremediation. Mycol. Res. 111, 3–49 (2007).
Fomina, M., Charnock, J. M., Hillier, S., Alvarez, R. & Gadd, G. M. Fungal transformations of uranium oxides. Environ. Microbiol. 9, 1696–1710 (2007).
Fomina, M., Burford, E. P., Hillier, S., Kierans, M. & Gadd, G. M. Rock-building fungi. Geomicrobiol. J. 27, 624–629 (2010).
Konhauser, K. O. & Urrutia, M. M. Bacterial clay authigenesis: a common biogeochemical process. Chem. Geol. 191, 399–413 (1999).
Gadd, G. M. Fungi in Biochemical Cycles (Cambridge Univ. Press, 2005).
Whiticar, M. J. Carbon and hydrogen isotope systematics of bacterial formation and oxidation of methane. Chem. Geol. 161, 291–314 (1999).
Drake, H., Tullborg, E.-L. & Mackenzie, A. B. Detecting the near-surface redox front in crystalline bedrock using fracture mineral distribution, geochemistry and U-series disequilibrium. Appl. Geochem. 24, 1023–1039 (2009).
Laaksoharju, M. et al. Hydrogeochemical evaluation and modelling performed within the Swedish site investigation programme. Appl. Geochem. 23, 1761–1795 (2008).
Yu, C., Drake, H., Mathurin, F. A. & Åström, M. E. Cerium sequestration and accumulation in fractured crystalline bedrock: the role of Mn-Fe (hydr-)oxides and clay minerals. Geochim. Cosmochim. Acta. 199, 370–389 (2017).
Larson, S. Å. et al. The Caledonian foreland basin in Scandinavia; constrained by the thermal maturation of the Alum Shale; discussion and reply. GFF 121, 155–159 (1999).
Cederbom, C., Larson, S. Å., Tullborg, E.-L. & Stiberg, J. P. Fission track thermochronology applied to Phanerozoic thermotectonic events in central and southern Sweden. Tectonophysics 316, 153–167 (2000).
Seal, R. R. II. Sulfur isotope geochemistry of sulfide minerals. Rev. Mineral. Geochem. 61, 633–677 (2006).
Wacey, D. et al. Nanoscale analysis of pyritized microfossils reveals differential heterotrophic consumption in the ∼1.9-Ga Gunflint chert. Proc. Natl Acad. Sci. USA 110, 8020–8024 (2013).
Raiswell, R. A geochemical framework for the application of stable sulphur isotopes to fossil pyritization. J. Geol. Soc. 154, 343–356 (1997).
Yarlett, N., Orpin, C. G., Munn, E. A., Yarlett, N. C. & Greenwood, C. A. Hydrogenosomes in the rumen fungus Neocallimastix patriciarum. Biochem. J. 236, 729–739 (1986).
O’Fallon, J. V., Wright, R. W. & Calza, R. E. Glucose metabolic pathways in the anaerobic rumen fungus Neocallimastix frontalis EB188. Biochem. J. 274, 595–599 (1991).
Brul, S. & Stumm, C. K. Symbionts and organelles in anaerobic protozoa and fungi. Trends. Ecol. Evol. 9, 319–324 (1994).
Sim, M. S., Bosak, T. & Ono, S. Large sulfur isotope fractionation does not require disproportionation. Science 333, 74–77 (2011).
Hoek, J., Reysenbach, A.-L., Habicht, K. S. & Canfield, D. E. Effect of hydrogen limitation and temperature on the fractionation of sulfur isotopes by a deep-sea hydrothermal vent sulfate-reducing bacterium. Geochim. Cosmochim. Acta 70, 5831–5841 (2006).
Hallbeck, L. & Pedersen, K. Explorative analyses of microbes, colloids, and gases together with microbial modelling. Site description model. SDM-Site Laxemar. Report No. SKB Report R-08-109 (Stockholm, Sweden, 2009).
Lin, L.-H., Slater, G. F., Sherwood Lollar, B., Lacrampe-Couloume, G. & Onstott, T. C. The yield and isotopic composition of radiolytic H2, a potential energy source for the deep subsurface biosphere. Geochim. Cosmochim. Acta 69, 893–903 (2005).
Stevens, T. O. & McKinley, J. P. Lithoautotrophic microbia, ecosystems in deep basalt aquifers. Science 270, 450–454 (1995).
Pedersen, K. Metabolic activity of subterranean microbial communities in deep granitic groundwater supplemented with methane and H2. ISME J, 7, 839–849 (2012).
Lau, M. C. Y. et al. An oligotrophic deep-subsurface community dependent on syntrophy is dominated by sulfur-driven autotrophic denitrifiers. Proc. Natl Acad. Sci. USA 113, E7927–E7936 (2016).
Parkes, J. R. Review of the Geomicrobiological Aspects of SKB’s Licence Application for a Spent Nuclear Fuel Repository in Forsmark, Sweden. Swe. Rad. Safe. Auth. 2012, 10 (2012).
Pedersen, K. Analysis of copper corrosion in compacted bentonite clay as a function of clay density and growth conditions for sulfate-reducing bacteria. J. Appl. Microbiol. 108, 1094–1104 (2010).
Laaksoharju, M. et al. Bedrock hydrogeochemistry Laxemar. Site descriptive modelling SDM-Site Laxemar. SKB Report R-08-93. Report No. SKB Report R-08-93 (Stockholm, Sweden, 2009).
King, F., Lilja, C. & Vähänen, M. Progress in the understanding of the long-term corrosion behaviour of copper canisters. J. Nucl. Mater. 438, 228–237 (2013).
Bish, D. L., Vaniman, D. T., Chipera, S. J. & Carey, J. W. The distribution of zeolites and their effects on the performance of a nuclear waste repository at Yucca Mountain, Nevada, U.S.A. Amer. Mineral. 88, 1889 (2003).
Bish, D. L. in Natural Microporous Materials in Environmental Technology (eds Misaelides P., Macášek F., Pinnavaia T. J., & Colella C.) 177-191 (Springer Netherlands, 1999).
Thanks to the Swedish Nuclear Fuel and Waste Management Co. (SKB) and NOVA-FoU for giving access to drill cores from Laxemar. Thanks to L. Ilyinski and K. Lindén for assistance during SIMS-analysis and F. Marone for assistance at the TOMCAT beamline. This is NordSIM publication 511.
The authors declare no competing financial interests.
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Drake, H., Ivarsson, M., Bengtson, S. et al. Anaerobic consortia of fungi and sulfate reducing bacteria in deep granite fractures. Nat Commun 8, 55 (2017). https://doi.org/10.1038/s41467-017-00094-6
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