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Cell seeding accelerates the vascularization of tissue engineering constructs in hypertensive mice

Hypertension Research volume 44, pages 23–35 (2021)Cite this article

Abstract

Rapid blood vessel ingrowth into transplanted constructs represents the key requirement for successful tissue engineering. Seeding three-dimensional scaffolds with suitable cells is an approved technique for this challenge. Since a plethora of patients suffer from widespread diseases that limit the capacity of neoangiogenesis (e.g., hypertension), we investigated the incorporation of cell-seeded poly-L-lactide-co-glycolide scaffolds in hypertensive (BPH/2J, group A) and nonhypertensive (BPN/3J, group B) mice. Collagen-coated scaffolds (A1 and B1) were additionally seeded with osteoblast-like (A2 and B2) and mesenchymal stem cells (A3 and B3). After implantation into dorsal skinfold chambers, inflammation and newly formed microvessels were measured using repetitive intravital fluorescence microscopy for 2 weeks. Apart from a weak inflammatory response in all groups, significantly increased microvascular densities were found in cell-seeded scaffolds (day 14, A2: 192 ± 12 cm/cm2, A3: 194 ± 10 cm/cm2, B2: 249 ± 19 cm/cm2, B3: 264 ± 17 cm/cm2) when compared with controls (A1: 129 ± 10 cm/cm2, B1: 185 ± 8 cm/cm2). In this context, hypertensive mice showed reduced neoangiogenesis in comparison with nonhypertensive animals. Therefore, seeding approved scaffolds with organ-specific or pluripotent cells is a very promising technique for tissue engineering in hypertensive organisms.

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Acknowledgements

The authors are grateful for the excellent technical assistance of Marie Luise Jenzer and Stefanie Rausch.

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Authors and Affiliations

  1. Division of Cranio-Maxillo-Facial and Oral Surgery, University Hospital Zurich, University of Zurich, Frauenklinikstrasse 24, 8091, Zurich, Switzerland

    Maximilian E. H. Wagner, Kathrin Schumann-Moor, Martin Rücker, Martin Lanzer, Thomas Gander, Harald Essig & Paul Schumann

  2. Department of Oral and Maxillofacial Surgery, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany

    Andreas Kampmann, Nils-Claudius Gellrich, Frank Tavassol & Friederike Schmeltekop

  3. Schuhstraße 40, 31134, Hildesheim, Germany

    Friederike Schmeltekop

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Wagner, M.E.H., Kampmann, A., Schumann-Moor, K. et al. Cell seeding accelerates the vascularization of tissue engineering constructs in hypertensive mice. Hypertens Res 44, 23–35 (2021). https://doi.org/10.1038/s41440-020-0524-z

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