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High-salt intake increases TRPC3 expression and enhances TRPC3-mediated calcium influx and systolic blood pressure in hypertensive patients

Hypertension Research volume 43, pages 679–687 (2020)Cite this article

Abstract

Enhanced transient receptor potential canonical subtype 3 (TRPC3) expression and TRPC3-mediated calcium influx in monocytes from hypertensive rats and patients are associated with increased blood pressure. Daily salt intake is closely related to hypertension, but the relationship between TRPC3 expression and salt intake has not yet been evaluated in hypertensive patients. Using reverse transcription-polymerase chain reaction, we studied the expression of TRPC3 and TRPC3-related store-operated calcium entry (SOCE) in peripheral blood mononuclear cells (PBMCs) from hypertensive and normotensive control subjects. Measurement of SOCE was performed using the fluorescent dye Fura-2 AM. Participants were divided into a low-salt group (<9 g) and a high-salt group (≥9 g) based on 24-h urinary sodium excretion. Increased TRPC3 mRNA expression levels and SOCE were observed in THP-1 cells after high-NaCl treatment. However, administration of the TRPC3-specific inhibitor Pyr3 significantly decreased the effect. Furthermore, the TRPC3 mRNA expression levels in PBMCs from high-salt intake patients with essential hypertension were significantly higher than those in low-salt intake patients compared with those in normotensive control subjects. We also observed significantly increased TRPC3-mediated SOCE in PBMCs from hypertensive subjects (but not from normotensive control subjects), with calcium concentration correlating with salt intake. More importantly, TRPC3 mRNA levels showed a significant correlation with salt intake and systolic blood pressure in patients with essential hypertension. This study demonstrated, for the first time, that increased TRPC3 mRNA levels are associated with elevated salt intake and systolic blood pressure in hypertensive patients.

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Acknowledgements

We thank Lijuan Wang, Peng Gao, Tingbing Cao and Hua Yang for their technical assistance. This study was supported by grants from the National Natural Science Foundation of China (81770416, 81630015, 81721001, 81600331) and National Key R&D Program of China (2018YFA0800601).

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  1. These authors contributed equally: Yingru Hu, Weijie Xia

Authors and Affiliations

  1. Department of Endocrinology, Yongchuan Hospital of Chongqing Medical University, Chongqing, 402160, China

    Yingru Hu, Cui Zhou & Zhiyong Li

  2. Department of Burn and Plastic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

    Weijie Xia & Hengshu Zhang

  3. Department of Hypertension and Endocrinology, Center for Hypertension and Metabolic Diseases, Daping Hospital, Third Military Medical University, Chongqing Institute of Hypertension, Chongqing, 400042, China

    Yingsha Li, Qianran Wang, Shaoyang Lin, Bin Wang, Yuanting Cui, Yanli Jiang, Xiaona Pu, Xiao Wei, Hao Wu, Zhiming Zhu & Daoyan Liu

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Correspondence to Daoyan Liu or Zhiyong Li.

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Hu, Y., Xia, W., Li, Y. et al. High-salt intake increases TRPC3 expression and enhances TRPC3-mediated calcium influx and systolic blood pressure in hypertensive patients. Hypertens Res 43, 679–687 (2020). https://doi.org/10.1038/s41440-020-0409-1

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