Using genetics to understand biology

The 100th Anniversary of the Genetics Society is a time to celebrate how much this distinguished and congenial Society has contributed to successive generations of geneticists at all stages of their research careers. It is also a time to celebrate genetics itself, a discipline that is both powerful and elegant, and that has provided much insight into the nature of life and how it works.

There are three important pillars of genetics, which permeate all aspects of our understanding of how living organisms function and evolve, and how the processes of life can be investigated. The first pillar is transmission genetics, the basis of heredity. Central to this pillar is the concept of the gene, first proposed by Mendel based on his brilliant abstract analysis and experimentation and championed by William Bateson (1901), even if Fisher’s (1936) subsequent statistical analysis suggested that the experimental data were perhaps just too good. The second pillar is how an organism’s genotype determines its phenotype. This is a problem of how information stored in the gene influences the phenotypic characteristics of an organism. This is essentially a coding problem as suggested by Watson and Crick (1953), who with their exceptional insight realised that the DNA making up the genes could act as, what we would now call, a digital information storage device. DNA sequences determine protein sequences and thus the structure and properties of the proteins that are responsible for phenotype. Combining these two pillars is informative about how living organisms work and how they come about during evolution. The third pillar is concerned with how genetics can be used to investigate the processes underpinning life.

It is this third pillar that is the subject of this article. Genetics methodologies provide powerful ways to investigate biological processes, and can ultimately reveal the underlying molecular mechanisms involved even when there is no knowledge at the outset of a study as to the mechanistic basis of a biological phenomenon. Our discussion will cover in general terms how genetics can be used to investigate how living organisms work, but for practical examples it draws primarily on work from the fission yeast Schizosaccharomyces pombe, based on our studies of the eukaryotic cell cycle. In Boxes throughout the text we describe in a more anecdotal way how some of these discoveries were made. The fission yeast was developed as a model organism in the 1950s and 1960s by Urs Leupold and Murdoch Mitchison (Hoffman et al. 2015), two outstanding scientists and generous advisors to PN.

So how has genetics helped to unravel processes and phenomena central to biology? Knowledge of the basis of heredity was extended beyond Mendel particularly by Bateson (1905) using the sweet pea, Lathyrus odoratus and Morgan (1910) and his colleagues (1915) using the fruit fly Drosophila melanogaster. Major understanding of the generation of phenotype from genotype came from Beadle and Tatum with their research into the fungus Neurospora crassa, work that led to the formulation of the one gene one enzyme hypothesis (Beadle and Tatum 1941). Principles of animal and plant development were established by research in many different organisms, but especially important were Drosophila (Morgan 1910; Morgan et al. 1915), the nematode worm Caenorhabditis elegans, developed as a model genetic organism by Brenner (1972), and the cress weed Arabidopsis thaliana (Feenstra 1964). A beautiful example of the power of developmental genetics are the studies in Drosophila of homeotic genes that when mutated can alter developmental fate, such as changing whether a leg or an antenna is formed in a particular location on the fly (Lewis 1978; Struhl 1981). Understanding the logical basis of gene regulation was explored by Jacob and Monod (1961) using the bacterium Escherichia coli, whereas understanding of neural development has drawn heavily on studies in Caenorhabitis (Bargmann 1998) as well as of the mouse, Mus musculus (Ellenbroek and Youn 2016). Mechanisms for a variety of eukaryotic cell biological phenomena have been revealed by research in the yeasts, including regulation of the cell cycle (Hartwell et al. 1970; 1973; Nasmyth and Nurse 1981; Nurse et al. 1976), secretion (Novick and Schekman 1979) and autophagy (Takeshige et al. 1992; Thumm et al. 1994).

These are just a few examples of the many processes illuminated by the application of genetic analyses. Most of these studies have been carried out with model genetic organisms characterised by ease of manipulation, short-generation times, ability to be mated, mutagenized and screened, and a suitability for molecular genetics. The topic of model organisms amenable to genetic analysis is covered more fully by Jonathan Hodgkin elsewhere in this volume.

Genetic screens

Forward genetic screens are usually the starting point for the genetic investigation of biological phenomena. The principle behind this type of screen is to search for mutants that will be informative about the process of interest. This requires procedures that allow efficient mutagenesis, easy screening, and the rapid detection of recessive mutations, either by using haploid and hemizygous organisms or via homozygosis of mutations in diploids. However, the very first step needs to be an act of creative imagination. What mutant phenotype can be imagined that will be informative about the process under study? This is crucial if the subsequent mutant screen is to be successful. A mutant screen is an exercise in pathology, a hunt for mutants with abnormal behaviours that disturb the process but at the same time are revealing about the normal functioning of that process. To make good choices about an appropriate mutant screen requires good knowledge about the biology of the model organism being used. As Barbara McClintock aptly put it, what is needed is a ‘feeling for the organism’ (pers com. PN). Genetics is derided by some for being too reductionist, but in fact the opposite is true; successful genetic studies need a good understanding of how the biological entity under investigation behaves as a whole, be it a cell, an organ, or an organism.

The use of forward genetic screens in the fission yeast S. pombe for study of the cell cycle and its control, provides a useful case study. Fission yeast is a single-celled haploid eukaryotic organism, a rod-shaped cylinder that grows by cell elongation at the tips (Fig. 1a). At the beginning of their cell cycle, wild type cells undergo a short G1 followed by S-phase. A subsequent long G2 ends with mitosis and is followed by cytokinesis, where a centrally placed septum divides the cell into two equally sized daughter cells. For genetic studies of the cell cycle, mutants need to be identified that are unable to complete the cell cycle successfully, and thus cannot undergo cell division. Such mutants will be lethal in a haploid organism, so the mutant phenotype needs to be conditional, which is the failure to complete cell division only in certain restrictive conditions allowing the cells to be kept alive when grown in the permissive conditions. The approach used was to mutagenize haploid cells to generate mutations, and then to screen for mutants that had a temperature sensitive phenotype because they had a thermolabile protein that was dysfunctional at high temperature.

Fig. 1: Cell cycle (cdc) mutant.
figure1

a A cdcts mutant at the permissive temperature. Cells can grow and divide and do not elongate. b A cdcts mutant at the restrictive temperature is unable to divide but continues to grow and so has an elongated cell phenotype. The cell wall and septum are stained with calcofluor

But what phenotypes should be screened for that are relevant to the cell cycle? The search is for mutant cells that cannot divide, but the problem is that there are many ways of stopping a cell from dividing, most of which are not informative because they are not directly involved in the cell cycle. Any gene required for the growth of the cell which is rendered non-functional will also block cell division if made non-functional by mutation. This includes defects in protein, RNA and other macromolecular synthesis, as well as metabolism and energy production, in fact most of the functions needed for the life of the cell. This problem can be overcome by selecting temperature sensitive mutants, which cannot complete the cell cycle but that are still able to grow because activities required for growth are still taking place. These rod-shaped fission yeast cells continue to grow but do not divide and thus form elongated cells which can be identified by microscopic visual screening (Fig. 1) (Nurse et al. 1976). This visual screen was carried out, and the first search isolated 27 mutants, called cdc for cell division cycle, which were found to define a total of 14 genes in complementation tests (Nurse et al. 1976). A second similar screen isolated 59 mutants defining a total of 10 further genes (Nasmyth and Nurse 1981). These genes formed the basis of initial work on the cell cycle, showing that well designed forward genetic screens can identify genes required for a biological process of interest and open it up for study.

The unexpected phenotype

The random forward genetic screens also have the potential to bring about serendipitous discovery, and designing screens that are somewhat open ended can assist such chance outcomes. Visual screens of the type used to find cell cycle mutants in fission yeast provide a chance to discover mutants that are different from what was originally conceived. In one sense, such open-ended searches allow ‘nature’ to deliver unexpected mutant phenotypes to any geneticist ready to recognise such opportunities.

Such serendipity had a hand in uncovering the role of the cdc2 gene at the G1–S transition. At the time, cdc2ts mutants were thought only to arrest at the G2–M transition and were used as a negative control for a screen to identify cdcts mutants that were blocked in G1 before commitment to the mitotic cell cycle and thus could still undergo sporulation. The cdc2ts mutant consistently showed a low level of sporulation at the restrictive temperature. Further experiments showed that in fact Cdc2 function was also required for the G1–S transition (Nurse and Bissett 1981). The majority of cdc2ts cells were blocking in G2 with a small percentage blocking in G1, and it was these G1 cells that were able to undergo conjugation and sporulation because they were blocked before commitment to the cell cycle. Demonstrating that a single-gene function was required at two completely different control points in the cell cycle was a significant step forward. (Box 1—Believing data).

Another example of chance discovery was the finding of a micro-colony of small cells during a screen for elongated cell cycle mutants (Box 2—Serendipity). Seeing such cells led to the realisation that they were being advanced into mitosis and cell division, before they grew to the normal size for entry into mitosis. This small cell phenotype (Fig. 2) revealed that there were rate-limiting steps acting in the cell cycle, one of which controlled the timing of the G2 to mitosis transition, providing new insight into cell cycle control. Following this chance observation, a systematic screen for small cell mutants was carried out (Thuriaux et al. 1978). The mutants were called wee (meaning small in Scotland) because they were first observed in Edinburgh. Two genes were identified, wee1 and cdc2 (originally called wee2), now known to encode CDK1, which is the name for cdc2 orthologues in all eukaryotic organisms. (Box 3—Throwing mutants away). Wee1 acts negatively and Cdc2 positively at the G2–M transition (Nurse and Thuriaux 1980). The wee phenotype of cdc2 was a consequence of a gain of function mutation, which would not have been found by screening a genome wide deletion collection (described in the next section).

Fig. 2: Wee mutants.
figure2

a Wild-type cells. b Wee mutant cells that are advanced into mitosis and divide at a small cell size. The cell wall and septum are revealed by dark field microscopy

Once the logic of advancement through the cell cycle was revealed as useful for understanding cell cycle control, it could be applied to other cell cycle events, such as the control of S-phase. Screens to identify mutants that could advance cells into S-phase produced mutant cells with a very unexpected phenotype- elongated cells with huge nuclei that had a high DNA content (Fig. 3). The phenotype was caused by overexpression of either cdc18 (cdc6 in other organisms) or rum1, advancing cells into S-phase and leading to DNA re-replication and thus higher ploidy (Moreno and Nurse 1994; Nishitani and Nurse 1995). These genes and another gene cdt1 (Hofmann and Beach 1994) were found to be core to the control acting over the onset of S-phase (Nishitani et al. 2000; Yanow et al. 2001). A deletion of the cyclin B cdc13 gene also caused this phenotype and it was subsequently shown that the Cdc13—CDK1 complex, required for entry into mitosis, was also required to prevent a further round of DNA replication from taking place from G2, thus ensuring that there is only one S-phase each cell cycle (Hayles et al. 1994).

Fig. 3: Cells undergoing DNA re-replication in the absence of mitosis.
figure3

Cells undergoing repeated rounds of DNA replication in the absence of mitosis produce elongated cells with increased ploidy. The nuclei are stained with the DNA-specific dye DAPI

Serendipitous discoveries such as these have been found in many different organisms and can be extremely useful in opening up new understanding of biological phenomena.

Systematic genomic screens

Forward mutagenesis based on the screens described above have proved to be very informative about a process but are neither systematic nor comprehensive. In contrast, systematic genome -wide screens allow the identification of a more complete catalogue of gene functions that are involved in the biological process of interest. Such screens are usually based on genetic approaches that eliminate or downregulate gene functions, so by definition will only identify genes that generate the mutant phenotype when they lose or reduce function. This is a limitation because gain of function mutants can be very illuminating, but this shortcoming is offset by the comprehensive nature of the screen. Molecular genetics can be used to systematically delete gene functions on a genome -wide basis using homologous recombination, an approach that has been valuable with bacteria (Baba et al. 2006) and the yeasts (Baudin et al. 1993; Giaever et al. 2002; Kim et al. 2010; Winzeler et al. 1999) and to some extent with multicellular organisms (Frokjaer-Jensen et al. 2010; Gong and Golic 2004). Alternative approaches include genome -wide systematic reduction of gene expression through RNAi knockdowns (Dietzl et al. 2007; Kamath and Ahringer 2003; Kiger et al. 2003) and overexpression screens that are useful for drug target screening (Arnoldo et al. 2014). CRISPR-Cas is also a useful gene-eliminating methodology, particularly for multicellular organisms (Dickinson and Goldstein 2016; Doudna and Charpentier 2014; Gratz et al. 2015). Its use and that of similar approaches will allow analysis of genome -wide gene deletions to be carried out in organisms that are less amenable to genetic studies.

In the fission yeast, a genome-wide gene deletion collection was constructed using homologous recombination to delete 4836 (98.4%) of the 4914 protein coding genes annotated at the time (Kim et al. 2010). Essential gene deletions are maintained as heterozygous deletion diploids, and the haploid deletions can be derived from each of these diploids by sporulation followed by germination of the haploid spores. Of the 4836 deletions constructed, only 26% were found to be essential for cell viability under the growth conditions used. By visual screening, all gene deletions after germination, it was possible to identify the genes that caused cell elongation when deleted because they were unable to complete the cell cycle (Hayles et al. 2013). A total of 513 cell cycle genes were identified of which 341 cdc genes were essential, more than double the number previously identified, despite the fact that earlier conventional forward genetic screens had been carried out for over 40 years. Interestingly, it was mainly the previously identified genes that showed the strongest cdc phenotype. A further 172 gene deletion strains were identified, which are not essential for viability but are elongated at cell division, and therefore are delayed in completing the cell cycle, and so contribute to cell cycle progression. (Box 4—I am not a robot). A screen of ~ 3000 of the non-essential gene deletions for cells that divided at a small size, and are thus defective in the timing of the G2–M transition (Navarro and Nurse 2012) identified 18 genes that are likely to be rate-limiting for cell cycle progression (see—Understanding the networks).

These systematic genomic approaches have generated an almost complete catalogue of the genes required for successful cell division, identifying the majority of genes that need to be considered when thinking about the eukaryotic cell cycle and particularly control of the cell cycle. From this ‘naming of the parts’ exercise for the cell cycle, we concluded that ~ 10% of fission yeast genes have roles in the cell cycle.

Systematic genomic screens similar to this have been carried out using model organisms to study many other biological processes, for example, identifying genes affecting cell morphology in Drosophila (Kiger et al. 2003) or UV sensitivity in budding yeast (Birrell et al. 2001). Given the conservation of molecular mechanisms throughout the living world, work on model organisms like bacteria, yeasts, worms and flies is likely to be relevant to all eukaryotes including ourselves.

Becoming molecular and cellular

Genetics is generally rather abstract in the ways in which it reveals how things work. Biological phenomena and processes are described in terms of gene names, but do not provide mechanistic explanations that describe the nature of the molecules and the biochemical processes involved. To move from abstract explanations to biochemical mechanisms requires cloning the relevant genes. This is possible by genetically mapping the genes and determining their position within the genome followed by sequencing of the region, a methodology greatly helped by the availability of whole-genome sequences. Another approach can be used when efficient DNA transformation procedures are available. Gene libraries can be constructed and transformed into mutant cells to select for clones that rescue the mutant function. This is cloning by complementation (Fig. 4), and was the approach used to clone genes in the yeasts (Beach et al. 1982; Nasmyth and Reed 1980). (Box 5—Is it a contaminant?).

Fig. 4: Cloning by complementation.
figure4

A cdc2ts mutant is able to grow at the restrictive temperature when cells carry the human CDC2 gene on a plasmid. This gene is able to complement the yeast cdc2ts mutant function and cells can grow and divide to form colonies (white arrow heads). Cells that lose the plasmid are no longer able to divide, but continue to grow and form elongated cells (black arrow heads)

In fission yeast complementation, cloning was combined with whole genome sequencing and positional mapping to generate the sequences of the majority of cell cycle genes (Kohli et al. 1977; Wood et al. 2002) (Box 6—Cottage Industry). With the availability of gene sequences, it is possible to predict their putative molecular functions. Biochemical investigations of these molecular functions are facilitated by purification of the gene products; for example, through tagging the genes or raising antibodies via protein expression in bacteria and protein purification, or by peptide synthesis. With gene product purification, comes the ability to perform biochemical assays, providing the link between genetics and molecular mechanism. Using tagged genes or specific antibodies against gene products also allows the cellular locations and behaviour of gene products of interest to be determined. Many cell cycle genes in different organisms have been tagged, and the locations and levels of the tagged proteins have been monitored as cells proceed through the cell cycle. Combining molecular and cellular information leads to the development of mechanistic explanations of biological processes, linking molecules to phenotypes.

One of the advantages of toggling between genetic, cellular and molecular data is that it increases the robustness of explanations. Each of these spheres of investigation have strengths and weaknesses that can complement each other, generating different types of explanations both abstract and mechanistic, thus strengthening the understanding of biological phenomena and processes. This is considered further in the next section.

Describing the networks

The next stage in understanding is to generate the networks of interacting molecules, the interactome, which is responsible for the proper functioning of the biological processes. This requires comprehensive databases, examples being pombase.org, flybase.org, wormbase.org, yeastgenome.org and thebiogrid.org (Chervitz et al. 1999; Gramates et al. 2017; Howe et al. 2016; Lock et al. 2018; Stark et al. 2006). These databases contain a complete list or near-complete list of the genes together with gene product and genetic interactions derived from forward genetic and systematic genomic screens and biochemical analyses. The components encoded by these genes can be organised together to generate a network underlying the process under study, using programmes such as esyn.org (Bean et al. 2014) or string-db.org (Jensen et al. 2009). Two types of data are available to generate these networks, based on either physical interactions using techniques such as yeast two hybrid analysis, affinity pull downs, and mass spectroscopy, or genetic interactions revealed by analyses such as suppression, synergism and epistasis. These physical and genetic methodologies complement each other because of the conceptually different approaches they use, so there is extra confidence when similar conclusions are reached.

Once an interactome underpinning a biological process has been generated there can be a tendency amongst researchers to leave it there, although such descriptions of networks are usually insufficient for a proper understanding of how the process works. Having the protein sequences derived from the gene sequences available allows predictions of the biochemical functions associated with the gene products to be made. From these predictions, at least a partial understanding of the network of biochemical processes can be built up. But what really is required is to move from identifying the components and their biochemical activities, to comprehension of the basic principles and operational logic that are critical to the network of interest. This is difficult because there is no clear investigative pathway to follow, but we shall consider what is useful when tackling this problem.

Understanding the networks

A major question is what types of explanations lead to a meaningful understanding of the biological process or phenomenon of interest? One concept we have found useful is to consider the process under study in terms of the management of information, because this can help in moving from chemical description to biological understanding. Issues pertinent to the management of information are inputs of information into a process, the integration, processing and storage of information intrinsic to the process, and how information determines the subsequent output that bring about a particular outcome for that process. Two classical biological phenomena that illustrate this concept are the structure of DNA and regulation of the Lac operon. The structure of DNA describes how the atoms are positioned with respect to each other in the DNA molecule. However, biological understanding only emerges when the management of information is considered. The structure of DNA only made sense biologically when it was shown that it was essentially a digital information storage device (first proposed by Mikhail Neiman (1964)) that could be precisely copied, explaining both coding and the inheritance of information (Brenner et al. 1961; Crick et al. 1961; Leder and Nirenberg 1964; Meselson and Stahl 1958). Similarly, the behaviour of the Lac operon can be described in terms of the chemistry of the molecules involved and how they interact with each other to control gene expression. However, biological understanding only comes when it is recognised that information flow through the system results in a negative feedback loop, which regulates the level of the Lac operon expression (Jacob and Monod 1961). Cell cycle control in fission yeast has also profited from this thinking. Informational inputs to the cell cycle control acting over mitosis and cell division come from the increasing size of the cell as the cell cycle proceeds, and from monitoring whether the DNA is undamaged and fully replicated. This information is integrated at the level of the cyclin dependent protein kinase (CDK1) activity, and output from the cyclin B–CDK1 complex results in phosphorylation of proteins with key roles at the onset and progression through mitosis (Blethrow et al. 2008; Swaffer et al. 2016).

A second concept that needs to be considered is what is meant by control. Improved biological understanding of a process often comes from knowing how the controls operate in the process. Sometimes the term control is used rather loosely, for example, when it is thought that control is associated with any step that is necessary for a process to work, even though with such a view nearly all steps can be considered as controls. It is more useful to identify the major rate-limiting steps that contribute in a significant way to the rate at which a biological process occurs. Thinking about this with respect to the cell cycle had its origins with discussions about rate-limiting steps in metabolic pathways, which revealed that rate-limiting controls can be distributed among a number of different steps in a network (Kacser and Burns 1995). It is also important to realise that the steps which are rate limiting can change depending on the biological context. An experiment useful for thinking about rate-limiting steps is to undertake ‘local perturbation analysis’. This approach requires the rate of an individual step in a network to be varied by a small amount, and then for the consequences of that local perturbation on the rate of the overall process to be determined. A systematic way to undertake local perturbation experiments is to use a haploinsufficient approach. Studying genes of interest in a heterozygous deletion situation where the level of component is likely to be reduced by half, and to test how this affects the process of interest. Over 500 cell cycle genes of fission yeast were investigated to identify those that delayed or advanced cells into mitosis. This led to the identification of 17 haploinsufficient genes that have impacts on the overall progression through the cell cycle (Moris et al. 2016). The reasoning behind this analysis was that mutants delaying or advancing the rate at which cells proceeded through the cell cycle, would identify potential control points in the cell cycle. This work identified tyrosine phosphorylation of CDK1 as a critical rate-limiting step for the timing of entry into mitosis. This approach together with a screen of the non-essential deletion collection for wee mutants (Navarro and Nurse 2012) has identified a set of 33 genes whose activities are likely to be rate limiting for cell cycle progression.

A further way is to think about controls is as decision steps, such as commitment to a specific developmental fate or entry into the cell cycle. A biological decision is made within the network that leads to the process either taking place or not. An extension of this idea is the checkpoint control by which a cell determines whether it should inhibit or continue with a process (Hartwell and Weinert 1989). Understanding this has been important for cell cycle studies, particularly when applied to controls acting over the onset of mitosis when DNA replication is incomplete or DNA is damaged (al-Khodairy and Carr 1992). These checkpoint inputs were found to operate in fission yeast through inhibitory tyrosine phosphorylation of CDK1 (Rhind et al. 1997).

Concepts such as these are useful for giving biological meaning to an understanding of how a network brings about a particular process. To test these ideas further needs knowledge of the molecular steps in the network and the context of how they operate in the cell or organism. It requires detailed hypothesis testing, and experimentation that combines genetics, biochemistry and cell biology. As data accumulate, it should be possible to develop systematic, theoretical and in silico approaches. Knowledge of the biochemical activities associated with different steps in the network and how they interact with each other can be combined with knowledge of whether these combinations of activities generate logical modules critical to network operation. For example, GTPases and their associated regulators can act as switches, amplifiers and timers within a network. Extending such knowledge to the various component combinations that make up networks may assist working out how they operate (Karlebach and Shamir 2008).

Perhaps a more radical approach to understanding the networks underpinning a biological process or phenomenon is to simplify the network. The thinking here is that systematic screens can identify many of the components that need to be considered when working out how a biological network operates, but they are unlikely to all be of significant importance. As a consequence components can be identified that when removed simplify regulation, while still maintaining the core operations of the network. In principle, this allows attention to be focussed on the key elements necessary to maintain core operations, reducing the risk of being distracted by functions that are more peripheral. This was used in fission yeast cell cycle studies to demonstrate that the four cyclin–CDK1 complexes identified as having roles in the mitotic cell cycle and the six cyclin–CDK1 complexes in the meiotic cell cycle, can all be replaced by a single cyclin B-CDK1 (Coudreuse and Nurse 2010; Gutierrez-Escribano and Nurse 2015). This means that orderly progression through the cell cycle is not driven by a series of qualitatively different cyclin–CDK1s as is generally assumed, but can be brought about by the rising activity of a single monomeric cyclin B–CDK1 as cells proceed through the cell cycle (Swaffer et al. 2016). This approach identified the core principle underlying CDK1 regulation of the cell cycle as being based on controlled quantitative increase and decrease in CDK1 activity.

Post-script

In this article, we have tried to demonstrate how genetics can help to understand biological processes and phenomena. Key to this are powerful classical and molecular genetic methodologies, imaginative approaches, and the ability to move between genetics, biochemistry and cell biology. For this type of approach to work well, it requires a clear focus on the physiology of the organism under study and for the researcher to have a true ‘feeling for the organism’.

References

  1. al-Khodairy F, Carr AM (1992) DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe. EMBO J 11:1343–1350

  2. Arnoldo A, Kittanakom S, Heisler LE, Mak AB, Shukalyuk AI, Torti D et al. (2014) A genome scale overexpression screen to reveal drug activity in human cells. Genome Med 6:32

  3. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:2006 0008

  4. Bargmann CI (1998) Neurobiology of the Caenorhabditis elegans genome. Science 282:2028–2033

  5. Bateson W (1901) Experiments in plant hybridisation. J R Hortic Soc 26:1–32

  6. Bateson W, Saunders ER, Punnet, RC (1905). Further experiments on inheritance in sweet Peas and Stocks: preliminary account. Proc R Soc B 77: 236–238.

  7. Baudin A, Ozier-Kalogeropoulos O, Denouel A, Lacroute F, Cullin C (1993) A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res 21:3329–3330

  8. Beach D, Durkacz B, Nurse P (1982) Functionally homologous cell cycle control genes in budding and fission yeast. Nature 300:706–709

  9. Beach D, Nurse P (1981) High-frequency transformation of the fission yeast Schizosaccharomyces pombe. Nature 290:140–142

  10. Beadle GW, Tatum EL (1941) Genetic control of biochemical reactions in neurospora. PNAS 27:499–506

  11. Bean DM, Heimbach J, Ficorella L, Micklem G, Oliver SG, Favrin G (2014) esyN: network building, sharing and publishing. PLoS ONE 9:e106035

  12. Beggs JD (1978) Transformation of yeast by a replicating hybrid plasmid. Nature 275:104–109

  13. Birrell GW, Giaever G, Chu AM, Davis RW, Brown JM (2001) A genome-wide screen in Saccharomyces cerevisiae for genes affecting UV radiation sensitivity. Proc Natl Acad Sci USA 98:12608–12613

  14. Blethrow JD, Glavy JS, Morgan DO, Shokat KM (2008) Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates. Proc Natl Acad Sci USA 105:1442–1447

  15. Brenner S (1972) The genetics of Caenorhabditis elegans. Genetics 77:71–94

  16. Brenner S, Jacob F, Meselson M (1961) An unstable intermediate carrying information from genes to ribosomes for protein synthesis. Nature 190:576–581

  17. Chervitz SA, Hester ET, Ball CA, Dolinski K, Dwight SS, Harris MA et al. (1999) Using the Saccharomyces Genome Database (SGD) for analysis of protein similarities and structure. Nucleic Acids Res 27:74–78

  18. Coudreuse D, Nurse P (2010) Driving the cell cycle with a minimal CDK control network. Nature 468:1074–1079

  19. Crick FH, Barnett L, Brenner S, Watts-Tobin RJ (1961) General nature of the genetic code for proteins. Nature 192:1227–1232

  20. Dickinson DJ, Goldstein B (2016) CRISPR-based methods for Caenorhabditis elegans genome engineering. Genetics 202:885–901

  21. Dietzl G, Chen D, Schnorrer F, Su KC, Barinova Y, Fellner M et al. (2007) A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448:151–156

  22. Doudna JA, Charpentier E (2014) Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science 346:1258096

  23. Ellenbroek B, Youn J (2016) Rodent models in neuroscience research: is it a rat race? Dis Model Mech 9:1079–1087

  24. Feenstra WJ (1964) Isolation of nutritional mutants in Arabidopsis thaliana. Genetica 35:259–269

  25. Fisher RA (1936) Has Mendel’s work been rediscovered. Ann Sci 1:115–126

  26. Frokjaer-Jensen C, Davis MW, Hollopeter G, Taylor J, Harris TW, Nix P et al. (2010) Targeted gene deletions in C. elegans using transposon excision. Nat Methods 7:451–453

  27. Giaever G, Chu AM, Ni L, Connelly C, Riles L, Veronneau S et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418:387–391

  28. Gong WJ, Golic KG (2004) Genomic deletions of the Drosophila melanogaster Hsp70 genes. Genetics 168:1467–1476

  29. Gramates LS, Marygold SJ, Santos GD, Urbano JM, Antonazzo G, Matthews BB et al. (2017) FlyBase at 25: looking to the future. Nucleic Acids Res 45:D663–D671

  30. Gratz SJ, Harrison MM, Wildonger J, O’Connor-Giles KM (2015) Precise genome editing of drosophila with CRISPR RNA-guided Cas9. Methods Mol Biol 1311:335–348

  31. Gutierrez-Escribano P, Nurse P (2015) A single cyclin-CDK complex is sufficient for both mitotic and meiotic progression in fission yeast. Nat Commun 6:6871

  32. Hartwell LH, Culotti J, Reid B (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci USA 66:352–359

  33. Hartwell LH, Mortimer RK, Culotti J, Culotti M (1973) Genetic control of the cell division cycle in yeast: V. Genetic analysis of cdc mutants. Genetics 74:267–286

  34. Hartwell LH, Weinert TA (1989) Checkpoints: controls that ensure the order of cell cycle events. Science 246:629–634

  35. Hayles J, Fisher D, Woollard A, Nurse P (1994) Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex. Cell 78:813–822

  36. Hayles J, Wood V, Jeffery L, Hoe KL, Kim DU, Park HO et al. (2013) A genome-wide resource of cell cycle and cell shape genes of fission yeast. Open Biol 3:130053

  37. Hoffman CS, Wood V, Fantes PA (2015) An ancient yeast for young geneticists: a primer on the Schizosaccharomyces pombe Modelsystem. Genetics 201:403–423

  38. Hofmann JF, Beach D (1994) cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis. EMBO J 13:425–434

  39. Howe KL, Bolt BJ, Cain S, Chan J, Chen WJ, Davis P et al (2016) WormBase 2016: expanding to enable helminth genomic research Nucleic Acids Res 44:D774–D780

  40. Jacob F, Monod J (1961) Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol 3:318–356

  41. Jensen LJ, Kuhn M, Stark M, Chaffron S, Creevey C, Muller J et al (2009) STRING 8--a global view on proteins and their functional interactions in 630 organisms Nucleic Acids Res 37:D412–D416

  42. Kacser H, Burns JA (1995) The control of flux. Biochem Soc Trans 23:341–366

  43. Kamath RS, Ahringer J (2003) Genome-wide RNAi screening in Caenorhabditis elegans. Methods 30:313–321

  44. Karlebach G, Shamir R (2008) Modelling and analysis of gene regulatory networks. Nat Rev Mol Cell Biol 9:770–780

  45. Kiger AA, Baum B, Jones S, Jones MR, Coulson A, Echeverri C et al. (2003) A functional genomic analysis of cell morphology using RNA interference. J Biol 2:27

  46. Kim DU, Hayles J, Kim D, Wood V, Park HO, Won M et al. (2010) Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe. Nat Biotechnol 28:617–623

  47. Kohli J, Hottinger H, Munz P, Strauss A, Thuriaux P (1977) Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic analysis and induced haploidization. Genetics 87:471–489

  48. Leder P, Nirenberg MW (1964) Rna codewords and protein synthesis, III. On the nucleotide sequence of a cysteine and a leucine Rna codeword. Proc Natl Acad Sci USA 52:1521–1529

  49. Lewis EB (1978) A gene complex controlling segmentation in Drosophila. Nature 276:565–570

  50. Lock A, Rutherford K, Harris MA, Hayles J, Oliver SG, Bahler J et al. (2018). PomBase 2018: user-driven reimplementation of the fission yeast database provides rapid and intuitive access to diverse, interconnected information. Nucleic Acids Res 47:D821-D827.

  51. Meselson M, Stahl FW (1958) The replication of DNA in escherichia coli. Proc Natl Acad Sci USA 44:671–682

  52. Moreno S, Nurse P (1994) Regulation of progression through the G1 phase of the cell cycle by the rum1+gene. Nature 367:236–242

  53. Morgan TH (1910) Sex-limited inheritance in Drosophila. Science 32:120–122

  54. Morgan TH, Sturtevant AH, Muller HJ, Bridges CB (1915) The Mechanism of Mendelian Inheritance. Henry Holt, New York, NY

  55. Moris N, Shrivastava J, Jeffery L, Li JJ, Hayles J, Nurse P (2016) A genome-wide screen to identify genes controlling the rate of entry into mitosis in fission yeast. Cell Cycle 15:3121–3130

  56. Nasmyth K, Nurse P (1981) Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 182:119–124

  57. Nasmyth KA, Reed SI (1980) Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc Natl Acad Sci USA 77:2119–2123

  58. Navarro FJ, Nurse P (2012) A systematic screen reveals new elements acting at the G2/M cell cycle control. Genome Biol 13:R36

  59. Neiman M (1964) Some fundamental issues of microminiaturization. Radiotekhnika 1:3–12

  60. Nishitani H, Lygerou Z, Nishimoto T, Nurse P (2000) The Cdt1 protein is required to license DNA for replication in fission yeast. Nature 404:625–628

  61. Nishitani H, Nurse P (1995) p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast. Cell 83:397–405

  62. Novick P, Schekman R (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci USA 76:1858–1862

  63. Nurse P, Bissett Y (1981) Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast. Nature 292:558–560

  64. Nurse P, Thuriaux P (1980) Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe. Genetics 96:627–637

  65. Nurse P, Thuriaux P, Nasmyth K (1976) Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 146:167–178

  66. Rhind N, Furnari B, Russell P (1997) Cdc2 tyrosine phosphorylation is required for the DNA damage checkpoint in fission yeast. Genes Dev 11:504–511

  67. Stark C, Breitkreutz BJ, Reguly T, Boucher L, Breitkreutz A, Tyers M (2006) BioGRID: a general repository for interaction datasets Nucleic Acids Res 34:D535–D539

  68. Struhl G (1981) A homoeotic mutation transforming leg to antenna in Drosophila. Nature 292:635–638

  69. Swaffer MP, Jones AW, Flynn HR, Snijders AP, Nurse P (2016) CDK substrate phosphorylation and ordering the cell cycle. Cell 167:1750–1761 e1716

  70. Takeshige K, Baba M, Tsuboi S, Noda T, Ohsumi Y (1992) Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction. J Cell Biol 119:301–311

  71. Thumm M, Egner R, Koch B, Schlumpberger M, Straub M, Veenhuis M et al. (1994) Isolation of autophagocytosis mutants of Saccharomyces cerevisiae. FEBS Lett 349:275–280

  72. Thuriaux P, Nurse P, Carter B (1978) Mutants altered in the control co-ordinating cell division with cell growth in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 161:215–220

  73. Watson JD, Crick FH (1953) Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature 171:737–738

  74. Winzeler EA, Shoemaker DD, Astromoff A, Liang H, Anderson K, Andre B et al. (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285:901–906

  75. Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A et al. (2002) The genome sequence of Schizosaccharomyces pombe. Nature 415:871–880

  76. Yanow SK, Lygerou Z, Nurse P (2001) Expression of Cdc18/Cdc6 and Cdt1 during G2 phase induces initiation of DNA replication. EMBO J 20:4648–4656

Download references

Acknowledgements

We thank everyone in the Cell Cycle Lab for useful discussions and suggestions during the writing of this review. PN and JH are supported by the Francis Crick Institute (FC01121 that receives its core funding from Cancer Research UK (FC01121), the UK Medical Research Council (FC01121) and The Wellcome Trust (FC01121).

Author information

Correspondence to Jacqueline Hayles.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark