Two new secondary metabolites from a mangrove-derived fungus Cladosporium sp. JS1-2

Abstract

One new pentenoic acid derivative, named 1,1′-dioxine-2,2′-dipropionic acid (1) and a new natural product, named 2-methylacetate-3,5,6-trimethylpyrazine (2), along with six known compounds (38), were obtained from the Cladosporium sp. JS1-2, an endophytic fungus isolated from the mangrove Ceriops tagal collected in South China Sea. Their structures were elucidated by detailed analysis of comprehensive spectroscopic data, and the structure of 1 was further determined by X-ray diffraction analyses. 13C NMR chemical shifts of structure 2 was further determined by GIAO based 13C NMR chemical shifts calculations. Compounds 14 and 6 showed growth inhibition activities against newly hatched larvae of Helicoverpa armigera Hubner with the IC50 values ranging from 100 to 150 μg ml−1. Compounds 1, 2, 4, 6 and 7 showed moderate antibacterial activities against Staphylococcus aureus with the MIC values of 25.0, 12.5, 6.25, 1.25, and 6.25 μg ml−1, respectively.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 21662012, 41866005), Program for Innovative Research Team in University (No. IRT-16R19), Innovation project of university students (No. cxcyxj2018009) and Innovation project of Postgraduate (No. Hyb2018-26).

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Correspondence to Guang-Ying Chen.

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X-ray data of Compound 1

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