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Simpotentin, a new potentiator of amphotericin B activity against Candida albicans, produced by Simplicillium minatense FKI-4981


Simpotentin, a new potentiator of amphotericin B activity against Candida albicans and Cryptococcus neoformans, was isolated from the culture broth of Simplicillium minatense FKI-4981 by Diaion HP-20 column chromatography, centrifugal partition chromatography, and preparative HPLC. The structure of simpotentin was elucidated by spectroscopic analyses including NMR and MS. The compound has a mannose core to which two medium-chain fatty acids are linked. Simpotentin was found to potentiate amphotericin B activity against C. albicans by the microdilution method.

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We express our thanks to Dr. Kenichiro Nagai and Ms. Noriko Sato of the School of Pharmacy, Kitasato University for the measurements of NMR and mass spectra. This work was supported by JSPS KAKENHI Grant Numbers 16H05095 (to RU) and 21310146 (to HT).

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Correspondence to Hiroshi Tomoda.

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Uchida, R., Kondo, A., Yagi, A. et al. Simpotentin, a new potentiator of amphotericin B activity against Candida albicans, produced by Simplicillium minatense FKI-4981. J Antibiot 72, 134–140 (2019).

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