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Simpotentin, a new potentiator of amphotericin B activity against Candida albicans, produced by Simplicillium minatense FKI-4981

The Journal of Antibiotics volume 72, pages 134–140 (2019)Cite this article

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Abstract

Simpotentin, a new potentiator of amphotericin B activity against Candida albicans and Cryptococcus neoformans, was isolated from the culture broth of Simplicillium minatense FKI-4981 by Diaion HP-20 column chromatography, centrifugal partition chromatography, and preparative HPLC. The structure of simpotentin was elucidated by spectroscopic analyses including NMR and MS. The compound has a mannose core to which two medium-chain fatty acids are linked. Simpotentin was found to potentiate amphotericin B activity against C. albicans by the microdilution method.

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Acknowledgements

We express our thanks to Dr. Kenichiro Nagai and Ms. Noriko Sato of the School of Pharmacy, Kitasato University for the measurements of NMR and mass spectra. This work was supported by JSPS KAKENHI Grant Numbers 16H05095 (to RU) and 21310146 (to HT).

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Authors and Affiliations

  1. Microbial Chemistry and Medical Research Laboratories, Graduate School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan

    Ryuji Uchida, Ariko Kondo, Akiho Yagi, Keisuke Kobayashi & Hiroshi Tomoda

  2. Department of Natural Product Chemistry, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi, 981-8558, Japan

    Ryuji Uchida & Akiho Yagi

  3. Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan

    Kenichi Nonaka & Rokurou Masuma

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  1. Ryuji Uchida
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  2. Ariko Kondo
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Correspondence to Hiroshi Tomoda.

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Uchida, R., Kondo, A., Yagi, A. et al. Simpotentin, a new potentiator of amphotericin B activity against Candida albicans, produced by Simplicillium minatense FKI-4981. J Antibiot 72, 134–140 (2019). https://doi.org/10.1038/s41429-018-0128-x

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