This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 digital issues and online access to articles
$119.00 per year
only $9.92 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Pinaud, E. et al. The IgH locus 3’ regulatory region: pulling the strings from behind. Adv. Immunol. 110, 27–70 (2011).
Saintamand, A. et al. Elucidation of IgH 3’ region regulatory role during class switch recombination via germline deletion. Nat. Commun. 6, 7084 (2015).
Methot, S. P. & Di Noia, J. M. Molecular mechanisms of somatic hypermutation and class switch recombination. Adv. Immunol. 133, 37–87 (2017).
Chaudhuri, J. & Alt, F. W. Class-switch recombination: interplay of transcription, DNA deamination and DNA repair. Nat. Rev. Immunol. 4, 541–552 (2004).
Panchakshari, R. A. et al. DNA double-strand break response factors influence end-joining features of IgH class switch and general translocation junctions. Proc. Natl Acad. Sci. USA 115, 762–767 (2018).
Matthews, A., Zheng, S., DiMenna, L. J. & Chaudhuri, J. Regulation of immunoglobulin class-switch recombination: choregraphy of noncoding transcription, targeted DNA deamination, and long-range DNA repair. Adv. Immunol. 122, 1–57 (2014).
Pan-Hammarström, Q. et al. Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells. J. Exp. Med. 201, 189–194 (2005).
Björkman, A. et al. DNA-PKcs is involved in Ig class switch recombination in human B cells. J. Immunol. 195, 5608–5615 (2015).
Boyer, F. et al. CSReport: a new computational tool designed for automatic analysis of class switch recombination junctions sequenced by high-throughput sequencing. J. Immunol. 198, 4148–4155 (2017).
Ghazzaui, N., Issaoui, H., Saintamand, A., Boyer, F. & Denizot, Y. Analysis of IgD CSR junctions by high-throughput sequencing. Immunol. Lett. 188, 86–88 (2017).
Issaoui, H., Ghazzaui, N., Saintamand, A., Denizot, Y. & Boyer, F. IgD class switch recombination is not controlled through the immunoglobulin heavy chain 3’ regulatory region super-enhancer. Cell Mol. Immunol. 14, 871–874 (2017).
Issaoui, H., et al. The IgH 3’ regulatory region super-enhancer does not control IgA class switch recombination in the B1 lineage. Cell Mol. Immunol. in press (2018) https://doi.org/10.1038/cmi.2017.103.
Acknowledgements
This work was supported by ANR (project EpiSwitch-3’RR 2016). N.G. was supported by a grant from Association de Spécialisation et d’Orientation Scientifique (Lebanon) and the municipality of Khiam (Lebanon). H.I. is supported by CORC (FJA/NP 2015-109) and the University of Limoges. The authors are “Equipe Labellisée LIGUE 2018”. We thank the GénoLim platform and the Cytometry platform of the University of Limoges for sequencing and cell sorting. F.B. is supported by Fondation Partenariale de l’Université de Limoges and ALURAD.
Author information
Authors and Affiliations
Contributions
H.I. and N.G. contributed equally to this work. Y.D. designed the research. N.G. and H.I. performed the research. A.S., F.B., and Y.D. analyzed the data. N.G., H.I., F.B., and Y.D. wrote the paper.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Issaoui, H., Ghazzaui, N., Saintamand, A. et al. High-throughput sequencing reveals similar molecular signatures for class switch recombination junctions for the γ and α isotypes. Cell Mol Immunol 16, 90–92 (2019). https://doi.org/10.1038/s41423-018-0025-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41423-018-0025-z
This article is cited by
-
Molecular analysis of γ1, γ3, and α class switch recombination junctions in APOBEC3-deficient mice using high-throughput sequencing
Cellular & Molecular Immunology (2020)
-
Uracil-DNA glycosylase is not implicated in the choice of the DNA repair pathway during B-cell class switch recombination
Cellular & Molecular Immunology (2019)