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Data availability
Cryo-EM density maps of thiamine-, pyridoxamine-, and fedratinib-bound SLC19A3 have been deposited in the Electron Microscopy Data Bank under the accession codes EMD-38691, EMD-38692, and EMD-38693, respectively. Their atomic coordinates have been deposited in the Protein Data Bank under accession codes 8XV2, 8XV5, and 8XV9, respectively.
Change history
05 April 2024
A Correction to this paper has been published: https://doi.org/10.1038/s41422-024-00953-0
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Acknowledgements
We thank the cryo-EM platform at the School of Life Sciences of Peking University for cryo-EM data collection. We thank Shitang Huang at the National Center for Protein Sciences of Peking University for assistance with radioactive experiments. We thank the High-Performance Computing Platform of Peking University for computing support. This research was funded by the National Key R&D Program of China (2021YFA1302300 to Z.Z.) and the National Natural Science Foundation of China (32171201 to Z.Z.). The study was also supported by the Center for Life Sciences, School of Life Sciences of Peking University, the State Key Laboratory of Membrane Biology of China (to Z.Z.), and ALSAC (to C.-H.L.).
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Z.Z. conceived the research and supervised the project. Y.D., T.Z., and Y.W. performed the cloning. Y.D. and T.Z. prepared all the protein samples and collected the cryo-EM data. G.W., Y.D., and Z.Z. processed the EM data. Z.Z. built and refined the structural models. T.Z. and Y.D. performed the radiolabeled [3H]-thiamine uptake assay. S.P. carried out the [3H]-pyridoxine uptake experiments. Y.W. and Y.D. carried out the thermostability and GFP thermal shift assay. N.C. and C.S. performed the MD simulation studies. C.-H.L. assisted in model building and structural analysis. Z.Z. wrote the manuscript. All authors participated in manuscript preparation and revision.
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The original online version of this article was revised: during proofreading, the authors intended to update Fig. S1 in the Supplementary information. They inadvertently uploaded only Fig. S1 without uploading the full supplementary file.
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Dang, Y., Zhang, T., Pidathala, S. et al. Substrate and drug recognition mechanisms of SLC19A3. Cell Res (2024). https://doi.org/10.1038/s41422-024-00951-2
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DOI: https://doi.org/10.1038/s41422-024-00951-2