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Cryo-EM structure of the type III-E CRISPR-Cas effector gRAMP in complex with TPR-CHAT

Cell Research volume 32, pages 1128–1131 (2022)Cite this article

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Fig. 1: Cryo-EM structure of the gRAMP in complex with TPR-CHAT.

Data availability

The atomic coordinates of the gRAMP–crRNA–TPR-CHAT and gRAMP–crRNA–ssRNA–TPR-CHAT complexes have been deposited to the Protein Data Bank under the accession numbers 7Y8T and 7Y8Y, respectively. The corresponding maps have been deposited in the Electron Microscopy Data Bank under the accession numbers EMD-33685 and EMD-33686, respectively.

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Acknowledgements

We thank Anqi Zhang and Changyou Guo of the EM platform at the School of Life Science and Technology of Harbin Institute of Technology for sample screening and data collection. This research was funded by the National Natural Science Foundation of China (31825008 and 31422014), the Tencent Foundation, and Heilongjiang Touyan Team (HITTY-20190034) to Z.H., and the fellowship of China National Postdoctoral Program for Innovative Talents (BX20200110) to M.G.

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  1. These authors contributed equally: Shuo Wang, Minghui Guo, Yuwei Zhu.

Authors and Affiliations

  1. HIT Center for Life Sciences, School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, China

    Shuo Wang, Minghui Guo, Yuwei Zhu, Zhiying Lin & Zhiwei Huang

  2. Westlake Center for Genome Editing, Westlake Laboratory of Life Sciences and Biomedicine, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China

    Zhiwei Huang

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Contributions

S.W., M.G., and Z.L. prepared the samples and performed functional assays. S.W. and M.G. performed negative staining. Y.Z. and Z.H. performed cryo-EM data processing and model building. S.W., Y.Z., and Z.H. wrote the manuscript. Z.H. supervised the study and edited the manuscript.

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Correspondence to Zhiwei Huang.

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Wang, S., Guo, M., Zhu, Y. et al. Cryo-EM structure of the type III-E CRISPR-Cas effector gRAMP in complex with TPR-CHAT. Cell Res 32, 1128–1131 (2022). https://doi.org/10.1038/s41422-022-00738-3

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