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The atomic coordinates of the gRAMP–crRNA–TPR-CHAT and gRAMP–crRNA–ssRNA–TPR-CHAT complexes have been deposited to the Protein Data Bank under the accession numbers 7Y8T and 7Y8Y, respectively. The corresponding maps have been deposited in the Electron Microscopy Data Bank under the accession numbers EMD-33685 and EMD-33686, respectively.
Koskella, B. & Brockhurst, M. A. FEMS Microbiol. Rev. 38, 916–931 (2014).
Marraffini, L. A. & Sontheimer, E. J. Nat. Rev. Genet. 11, 181–190 (2010).
Makarova, K. S. et al. Nat. Rev. Microbiol. 18, 67–83 (2020).
Sander, J. D. & Joung, J. K. Nat. Biotechnol. 32, 347–355 (2014).
Fagerlund, R. D., Staals, R. H. & Fineran, P. C. Genome Biol. 16, 251 (2015).
Cox, D. B. et al. Science 358, 1019–1027 (2017).
van Beljouw, S. P. et al. Science 373, 1349–1353 (2021).
Özcan, A. et al. Nature 597, 720–725 (2021).
Taylor, D. W. et al. Science 348, 581–585 (2015).
Kato, K. et al. Cell 185, 2324–2337.e16 (2022).
Hu, C. et al. Science 377, 1278–1285 (2022).
Kato, K. et al. bioRxiv https://doi.org/10.1101/2022.08.17.504292 (2022).
We thank Anqi Zhang and Changyou Guo of the EM platform at the School of Life Science and Technology of Harbin Institute of Technology for sample screening and data collection. This research was funded by the National Natural Science Foundation of China (31825008 and 31422014), the Tencent Foundation, and Heilongjiang Touyan Team (HITTY-20190034) to Z.H., and the fellowship of China National Postdoctoral Program for Innovative Talents (BX20200110) to M.G.
The authors declare no competing interests.
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Wang, S., Guo, M., Zhu, Y. et al. Cryo-EM structure of the type III-E CRISPR-Cas effector gRAMP in complex with TPR-CHAT. Cell Res 32, 1128–1131 (2022). https://doi.org/10.1038/s41422-022-00738-3
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