Cryo-EM structure of human heptameric Pannexin 1 channel

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Cryo-EM structure of the human PANX1 channel.

References

  1. 1.

    Panchin, Y. et al. Curr. Biol. 10, R473–R474 (2000).

  2. 2.

    Bruzzone, R., Hormuzdi, S. G., Barbe, M. T., Herb, A. & Monyer, H. Proc. Natl. Acad. Sci. USA 100, 13644–13649 (2003).

  3. 3.

    Penuela, S., Harland, L., Simek, J. & Laird, D. W. Biochem. J. 461, 371–381 (2014).

  4. 4.

    Sang, Q. et al. Sci. Transl. Med. 11, eaav8731 (2019).

  5. 5.

    Boassa, D. et al. J. Biol. Chem. 282, 31733–31743 (2007).

  6. 6.

    Chekeni, F. B. et al. Nature 467, 863–867 (2010).

  7. 7.

    Dahl, G. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 370, 20140191 (2015).

  8. 8.

    Isakson, B. E. & Thompson, R. J. Channels 8, 118–123 (2014).

  9. 9.

    Wang, J. & Dahl, G. Am. J. Physiol. Cell Physiol. 315, C290–C299 (2018).

  10. 10.

    Chiu, Y. H. et al. Nat. Commun. 8, 14324 (2017).

  11. 11.

    Bunse, S. et al. J. Membr. Biol. 244, 21–33 (2011).

  12. 12.

    Sandilos, J. K. et al. J. Biol. Chem. 287, 11303–11311 (2012).

Download references

Acknowledgements

We are grateful to Dr Qingxia Wang (Cryo-Electron Microscopy Research Center at Shanghai Institute of Materia Medica) and Dr Yu Kong (EM facility at the Institute of Neuroscience) for facility support, to Dr Xiang Zhang and Hong Wu (Shanghai YueXin Life-science Tech) for computational support, to Dr Shanshuang Chen (Shanghai Jiao Tong University) for data analysis, and to Dr Qing Sang (Fudan University) for mouse oocyte microinjection. This work was financially supported by the National Key R&D Program of China (2017YFA0505700, 2016YFC1000600, 2017YFC1001500, 2018YFC1003800), the National Natural Science Foundation of China (31771115, 81725006, 81822019, 81771581, 81571501, 81971450, 81971382), the Strategic Priority Research Program of the Chinese Academy of Science (XDB32020000, XDA12010317), the Shanghai Municipal Science and Technology Major Project (2018SHZDZX05, 2017SHZDZX01), the CAS Key Laboratory of Receptor Research (SIMM1804YKF-03), the Thousand Young Talents Program, the 100 Talents Program of the Chinese Academy of Sciences, the Natural Science Foundation of Shanghai (18ZR1447700), and Shanghai Rising-Star Program (grant 17QA1404800).

Author information

Affiliations

Authors

Contributions

R.Q., L.W., and S.Z. designed the project; R.Q. performed the protein purification and sample preparation; R.Q., L.D., and J.Z. collected the data; L.D. and X.Y. processed the cryo-EM images and built the model; and R.Q. and S.Z. analyzed the data and wrote the manuscript with inputs from all co-authors.

Corresponding authors

Correspondence to Xuekui Yu or Lei Wang or Shujia Zhu.

Ethics declarations

Competing interests

The authors declare no competing interests.

Supplementary information

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Qu, R., Dong, L., Zhang, J. et al. Cryo-EM structure of human heptameric Pannexin 1 channel. Cell Res 30, 446–448 (2020). https://doi.org/10.1038/s41422-020-0298-5

Download citation