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Buijs J, Hlady V. Adsorption kinetics, conformation, and mobility of the growth hormone and lysozyme on solid surfaces, studied with TIRF. J Colloid Interface Sci. 1997;190:171–81.
Krisdhasima V, McGuire J, Sproull R. Surface hydrophobic influences on β-lactoglobulin adsorption kinetics. J Colloid Interface Sci. 1992;154:337–50.
MacRitchie F. The adsorption of proteins at the solid/liquid interface. J Colloid Interface Sci. 1972;38:484–8.
Nakanishi K, Sakiyama T, Imamura K. On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon. J Biosci Bioeng. 2001;91:233–44.
Shirahama H, Suzawa T. Adsorption of bovine serum albumin onto styrene/acrylic acid copolymer latex. Colloid Polym Sci. 1985;263:141–6.
Zittle CA. Adsorption studies of enzymes and other proteins. Adv Enzymol Relat Areas Mol Biol. 1953;14:319–74.
Bos MA, Shervani Z, Anusiem AC, Giesbers M, Norde W, Kleijn JM. Influence of the electric potential of the interface on the adsorption of proteins. Colloids Surf B: Biointerfaces. 1994;3:91–100.
Goebel-Stengel M, Stengel A, Taché Y, Reeve JR Jr. The importance of using the optimal plasticware and glassware in studies involving peptides. Anal Biochem. 2011;414:38–46.
Manning MC, Patel K, Borchardt RT. Stability of protein pharmaceuticals. Pharm Res. 1989;6:903–18.
Jeyachandran Y, Mielczarski J, Mielczarski E, Rai B. Efficiency of blocking of non-specific interaction of different proteins by BSA adsorbed on hydrophobic and hydrophilic surfaces. J Colloid Interface Sci. 2010;341:136–42.
Hockings C, Anwari K, Ninnis R, Brouwer J, O’Hely M, Evangelista M, et al. Bid chimeras indicate that most BH3-only proteins can directly activate Bak and Bax, and show no preference for Bak versus Bax. Cell Death & Dis. 2015;6:e1735.
Hockings C, Alsop AE, Fennell SC, Lee EF, Fairlie WD, Dewson G, et al. Mcl-1 and Bcl-x L sequestration of Bak confers differential resistance to BH3-only proteins. Cell Death & Differ. 2018;25:719.
We thank Melissa Shi for technical assistance. This work was supported by NHMRC grants (1008434, 1016701), scholarship (Australian Postgraduate Award to CH), and by the Victorian State Government Operational Infrastructure Support and the Australian Government NHMRC IRIISS.
Conflict of interest
The authors declare that they have no conflict of interest.
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Hockings, C., Iyer, S., Uren, R.T. et al. Avoiding adsorption of Bcl-2 proteins to plasticware is important for accurate quantitation. Cell Death Differ 26, 794–795 (2019) doi:10.1038/s41418-019-0284-y