Abstract
In recent years, liquid biopsy has emerged as an alternative method to diagnose and monitor tumors. Compared to classical tissue biopsy procedures, liquid biopsy facilitates the repetitive collection of diverse cellular and acellular analytes from various biofluids in a non/minimally invasive manner. This strategy is of greater significance for high-grade brain malignancies such as glioblastoma as the quantity and accessibility of tumors are limited, and there are collateral risks of compromised life quality coupled with surgical interventions. Currently, blood and cerebrospinal fluid (CSF) are the most common biofluids used to collect circulating cells and biomolecules of tumor origin. These liquid biopsy analytes have created opportunities for real-time investigations of distinct genetic, epigenetic, transcriptomics, proteomics, and metabolomics alterations associated with brain tumors. This review describes different classes of liquid biopsy biomarkers present in the biofluids of brain tumor patients. Moreover, an overview of the liquid biopsy applications, challenges, recent technological advances, and clinical trials in the brain have also been provided.
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Acknowledgements
The authors thank The University of Texas MD Anderson Cancer Center, Houston, USA for providing the computational facilities and literature required to complete this review. The authors also gratefully acknowledge stimulating discussions with their colleagues Dr. Marcos Estecio and Dr. Chad Tang which helped in completing this review.
Funding
We would like to thank funding from MD Anderson Startup funds and a grant from the NCI 5R01 CA225963 to Krishna P Bhat for financial support.
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RT and KB established the review idea and finalized the framework. RT and KB were involved in the writing and editing of the review. All authors read and approved the final version of the manuscript.
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Trivedi, R., Bhat, K.P. Liquid biopsy: creating opportunities in brain space. Br J Cancer 129, 1727–1746 (2023). https://doi.org/10.1038/s41416-023-02446-0
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DOI: https://doi.org/10.1038/s41416-023-02446-0
