Abstract
Background
Plasmacytoid urothelial carcinoma (PUC) is a rare, aggressive histologic variant of urothelial cancer characterised by a diffuse growth pattern and CDH1 mutation. We studied the efficacy of preoperative platinum-based chemotherapy in nonmetastatic PUC and immune checkpoint inhibitors (ICIs) in advanced PUC.
Methods
Cases of nonmetastatic PUC and advanced PUC treated with ICIs at our institution were identified. Outcomes were compared to those of a published cohort of patients with urothelial carcinoma not otherwise specified.
Results
We identified 81 patients with nonmetastatic PUC. Of the patients with localised disease who underwent neoadjuvant chemotherapy, pathologic complete response and downstaging rates were 12 and 21%, respectively. Pathologic downstaging was not associated with significant improvement in clinical outcomes. Up to 18% of localised disease and 28% of locally advanced cases had unresectable disease at the time of surgery. ICI-treated advanced PUC (N = 21) had progression-free and overall survival of 4.5 and 10.5 months, respectively, and a 38% response rate. FGFR3 and DNA damage response gene alterations were observed in 3 and 15% of cases, respectively.
Conclusions
PUC is associated with high disease burden and poor chemosensitivity. Increased awareness and recognition of this disease variant will allow for new treatment strategies.
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Background
Plasmacytoid urothelial carcinoma (PUC) is a rare histologic variant of urothelial carcinoma (UC) characterised by an aggressive natural history and clinical course.1 It has a diffuse growth pattern2 and is more likely to be locally advanced at diagnosis than conventional UC (not otherwise specified [NOS]).3 Somatic truncating alterations in CDH1, which encodes the protein E-cadherin, have been reported in 80% of sequenced PUC cases; hypermethylation of the CDH1 promoter is found in other PUC cases.4 Although some data have suggested that PUC survival outcomes are not inferior to UC NOS when matched for disease stage at diagnosis, the rate of perioperative chemotherapy use is low in these analyses.5,6
Preoperative cisplatin-based chemotherapy increases pathologic responses and cancer-specific survival7 and is an integral component of modern management of UC. The presence of variant histology does not appear to compromise the efficacy of preoperative chemotherapy in patients with UC of squamous or glandular differentiation.8,9 However, the utility of preoperative chemotherapy for patients with PUC remains poorly defined. Anti-programmed cell death 1 (anti-PD1)/programmed cell death ligand 1 (PDL1) immune checkpoint inhibitors (ICIs) are also used to treat patients with UC10,11,12,13 and have an emerging role in preoperative therapy.14,15 However, the efficacy of these agents in PUC has not been studied.
In describing our institutional experience in the management of PUC, the primary objectives of this study are to (1) define the clinicopathologic features of PUC at diagnosis, (2) determine the response of PUC to preoperative platinum-based chemotherapy, and (3) further describe the genomic landscape of this under-studied disease entity. We also described the clinical activity of ICIs in a cohort of patients with advanced PUC.
Methods
This work was performed under an institutional review board–approved protocol. Cases of PUC of the bladder diagnosed at our institution were identified, and all cases were reviewed by a genitourinary pathologist (H.A.-A.) to confirm the diagnosis. Cases diagnosed between 2000 and 2017 with clinical follow-up were included for primary clinical analysis (Fig. 1). Only cases that were nonmetastatic at presentation were included in the study. All cases were classified as either localised PUC (cT2–T4a N0 disease) or locally advanced PUC (radiographic evidence of regional lymphadenopathy and/or fixed bladder at examination under anaesthesia). Tumours were classified according to the 2016 World Health Organisation Classification of Tumours of the Urinary System.1 Tumours were characterised by a diffuse and discohesive growth pattern with minimal stroma reaction. Tumour cells had eccentric or centrally located nuclei. Cytoplasmic vacuoles and signet ring cells were identified in all cases at variable proportions.
Pathologic stages were compared between patients with clinically localised PUC who underwent either neoadjuvant chemotherapy before cystectomy or cystectomy first. Outcomes were also compared between patients with PUC and patients with UC NOS. A cohort of patients with UC NOS (N = 146) treated with neoadjuvant cisplatin and gemcitabine for whom clinical outcome data have been reported was used as a comparator for this analysis.16
An expanded pathology database of cases of PUC diagnosed between 2000 and 2020 was used to identify cases that were genomically profiled (Supplementary Fig. S1). Tumours were sequenced using Memorial Sloan Kettering Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT), a Food and Drug Administration-authorised, hybridisation capture–based, next-generation sequencing platform.17,18 Genes that are sequenced in MSK-IMPACT were chosen based on documentation in the Catalogue of Somatic Mutations in Cancer19,20 and annotation of oncogenicity in the OncoKB Precision Oncology Knowledge Base (https://oncokb.org).21 Alterations in DNA damage response (DDR) genes were also evaluated. DDR status was classified based on the presence of mutations in 34 genes that are involved in different DDR pathways and are in the MSK-IMPACT sequencing panel. The expanded database was also used to identify patients with advanced PUC who received anti-PD1/PDL1 ICIs.
Fisher’s exact and Wilcoxon rank-sum tests were used to test the significance of differences of categorical and continuous data, respectively. Recurrence-free survival (RFS) was calculated from the date of radical cystectomy (or attempted surgery) to the date of radiographic evidence of recurrence or death. Patients whose cystectomies were aborted were excluded from RFS analysis. Overall survival (OS) was calculated from the date of radical cystectomy (or attempted surgery) to the date of death. Survival was estimated by Kaplan–Meier and compared by log-rank test. Cox proportional hazard models were used for univariate and multivariate analyses, and interactions were tested between histology (PUC vs UC NOS) and response (responders vs non-responders). Analyses were conducted with SAS version 9.4 (SAS institute Inc., Cary, NC). All tests were two sided, and p < 0.05 was considered significant.
Results
We identified 81 patients with nonmetastatic PUC (Fig. 1) who received treatment at our institution. The majority of patients (n = 62, 77%) had clinically localised disease; 19 (23%) patients had locally advanced disease at diagnosis, defined as the presence of fixed tumour at examination under anaesthesia or unequivocal radiographic evidence of locoregional lymphadenopathy. Baseline characteristics of the study population are listed in Table 1.
Clinically resectable, localised PUC
Of the 62 patients with localised muscle-invasive PUC, 29 (47%) underwent surgery first. Thirty-three (53%) patients received neoadjuvant chemotherapy, all of whom received cisplatin-based chemotherapy. Pathologic staging of these patients is shown in Table 2 and Supplementary Table S1. Although 97% of those who underwent up-front surgery had radiographic cT2 disease, 79% had pT3–4 disease on pathologic evaluation, and 38% had positive nodes. Twelve (41%) of the 29 patients in the surgery only group received adjuvant chemotherapy, of which 11 (92%) had pT3/4 disease and 6 (50%) had nodal involvement. In comparison, 12 (71%) of the 17 patients who did not receive adjuvant chemotherapy had pT3/4 disease and 5 (29%) had nodal involvement. (p = 0.06 for pT3–4 disease; p = 0.44 for nodal involvement).
Of the patients who received neoadjuvant chemotherapy, 10 (30%) patients received triplet chemotherapy regimen (cisplatin, gemcitabine, and paclitaxel), and 6 (18%) patients received more than four cycles of chemotherapy (up to six planned cycles). Cystectomy was aborted in 6 (18%) cases at the time of surgery due to locally advanced, unresectable disease following chemotherapy. All six patients had fixed bladders—three to the pelvic side wall, two to the intestines and/or rectum, and one to both. Three of these patients had positive biopsies that confirmed disseminated disease.
Following neoadjuvant chemotherapy, pathologic downstaging to non-muscle-invasive disease (<ypT2 ypN0) was observed in 7 (21%) patients, 4 (12%) of which had pathologic complete response. Of the remaining three patients, two had carcinoma in situ and one had pT1 disease. Triplet chemotherapy, extended number of chemotherapy cycles, or other clinical variables were not associated with response to neoadjuvant chemotherapy (Supplementary Table S2). Of the 23 patients with cT2 disease at diagnosis, 10 (43%) had ypT3–4 disease and 6 (26%) had nodal involvement at pathologic evaluation despite neoadjuvant chemotherapy. Three surgeries were aborted due to fixed bladder at attempted cystectomy.
Locally advanced PUC
Locally advanced PUC was defined as disease with regional lymphadenopathy and/or fixed tumour on examination under anaesthesia. Nineteen patients with locally advanced disease were identified, 18 of whom received systemic chemotherapy. The patient who did not receive systemic therapy underwent up-front surgery. Post-surgical pathology showed extensive bladder wall involvement along the entire circumference, with extensive invasion into the perivesical soft tissue, prostate, and seminal vesicles and positive surgical margins. Local recurrence was noted 4.6 months later.
Among those who received systemic therapy, 10 (56%) had locally advanced disease per examination under anaesthesia, 7 (39%) had radiographic evidence of regional lymphadenopathy, and 1 had both features. Most patients received cisplatin (n = 13, 72%), triplet chemotherapy regimen (n = 13, 72%), and >4 cycles of chemotherapy (n = 16, 89%). Although resection was attempted for every patient with locally advanced PUC after chemotherapy, the surgeries of 5 (28%) patients were aborted due to inoperable disease at intraoperative assessment.
Four (22%) patients had pathologic responses, with 2 (11%) pathologic complete responses. Median RFS and OS for patients with locally advanced PUC were 5.7 and 10.6 months, respectively, with 19% of patients alive at 2 years. The OS of patients whose tumours were found unresectable during surgery was significantly shorter than the OS of those who underwent resection (6.0 and 19.6 months, respectively; hazard ratio [HR] 3.57; 95% confidence interval [CI] 0.76–16.65, p = 0.009).
Comparison of PUC and UC NOS clinical outcomes
A previously published cohort of patients with muscle-invasive UC NOS of the bladder treated with neoadjuvant cisplatin and gemcitabine (n = 146) was used as comparator for RFS and OS.16 The percentage of patients with pathologic complete response was lower in patients with PUC as compared to patients with UC NOS, but this difference was not statistically significant (12 vs 21%, p = 0.456). The percentage of patients with pathologic downstaging (<ypT2 pN0) was significantly lower in patients with PUC than in patients with UC NOS (21 vs 45%, p = 0.018).
Patients with PUC treated with neoadjuvant cisplatin-based chemotherapy had significantly inferior RFS and OS than patients with UC NOS who received neoadjuvant chemotherapy (median RFS 16.7 months vs not reached [NR], log-rank p < 0.001; median OS 30.2 months vs NR, log-rank p < 0.004).
RFS and OS were also compared between PUC and UC NOS based on pathologic responses (Fig. 2a, b). For responders, PUC patients had worse RFS (HR 10.17, 95% CI 2.26–45.72) and OS (HR = 11.47, 95% CI 2.55–51.57). For non-responders, RFS (HR = 1.16, 95% CI 0.66–2.03) and OS (HR = 1.20, 95% CI 0.66–2.16) were comparable.
One of the 4 patients with pathologic complete response experienced disease recurrence after 15.5 months, with an overall survival of 22.2 months. The other three remained recurrence-free after 10.4, 14.4, and 29.9 months of follow-up. Of the 3 patients with residual non-muscle invasive disease, 2 (67%) had relapses: one at 3.2 months, who died after 3.5 months, and another at 17.6 months, who died after 40.9 months. The third patient remained recurrence-free at 21.9 months follow-up.
Patterns of failure
Of the 29 patients with clinically localised PUC treated with cystectomy first, 11 (38%) patients had documented recurrences—5 (45%) osseous, 4 (36%) nodal, 2 (18%) peritoneal, and 1 (9%) pelvic. One patient had two sites of disease at first recurrence. Of the 4 nodal recurrences, 3 (75%) were retroperitoneal lymphadenopathy.
Of the patients with clinically localised PUC who received neoadjuvant chemotherapy, 17 patients (52%) had documented recurrences—sites of recurrence included: 4 (24%) nodal (all retroperitoneal), 4 (24%) peritoneal, 4 (24%) local, 3 (18%) osseous, 2 (12%) hepatic, 2 (12%) pulmonary, and 1 (6%) brain.
Of those with locally advanced disease treated with chemotherapy, cystectomy was aborted in 5 (28%) cases. The most common patterns of failure were peritoneal (7 patients, 39%), osseous (2 patients, 11%), nodal (1 patient, 6%), hepatic (1 patient, 6%), and pulmonary (1 patient, 6%). Three cases of peritoneal recurrence presented as bowel obstruction.
Anti-PD1/PDL1 immune checkpoint blockade in advanced PUC
Between September 2014 and May 2020, 21 patients with advanced PUC were treated with ICIs (19 with anti-PD1/PDL1 monotherapy and 2 with anti-PD1 and anti-CTLA4 combination). Baseline characteristics are tabulated in Supplementary Table S3.
Among the 19 patients with advanced PUC, 6 (32%) experienced radiographic response. Median progression-free survival and OS were 4.5 and 10.5 months, respectively (Supplementary Fig. S1). Median duration of response was 17.0 months, with 2 on-going responses at 26.0 and 66.5 months at the time of analysis.
The two patients who received combination therapy had on-going responding disease for 4.5 and 7.2 months at the time of data analysis. Responses were observed in 8/21 patients (38%, including both patients treated with combination therapy).
Genomic characterisation of PUC
Thirty-three tumours in our database of 122 pathologically reviewed and confirmed cases of PUC were sequenced using MSK-IMPACT (Supplementary Fig. S2). None of these tumours had been sequenced in our previous analysis.4 Deleterious CDH1 alterations were noted in 20/33 (61%) cases.
Median tumour mutation burden (TMB) for the cohort was 14.9 mutations/Mb (range 1.8–45.6; interquartile range 8.9–21.6); this represents the 75th percentile among all UC (UC NOS and variant histologies) in our MSK-IMPACT database (N = 1961 samples as of June 2020). For the ICI-treated subset, 16/21 (76%) had MSK-IMPACT performed, with median TMB of 18.4 mutations/Mb (range 3.3–45.6; interquartile range 8.9–26.6). The median TMB for ICI monotherapy responders and non-responders was 15.3 and 14.3 mutations/Mb, respectively (p = 0.8).
Alterations in DDR genes have been shown to be associated with platinum responsiveness.22,23 Five (15%) tumours harboured six alterations in DDR genes: ERCC2 (I174M and I444M), ATM (E38*, K196*, and E2904Dfs*29), BRCA2 (E23Vfs*17), and RECQL4 (X822_splice). Neither ERCC2 alteration has been functionally validated, but the mutated residues are at or near the helicase domains.
Although the genes mutated in PUC were not dissimilar to those mutated in UC NOS,24 we observed higher rates of alterations in TP53 (25 cases, 76 vs 48%, p = 0.006) and RB1 (18 cases, 55 vs 17%, p < 0.001) compared to UC NOS from The Cancer Genome Atlas data set.25 Only 1 (3%) case in the PUC cohort harboured an alteration in FGFR3 (Fig. 3).
Discussion
PUC is an aggressive variant of UC that is characterised by poor clinical outcomes and is frequently locally advanced at diagnosis. Our series represents one of the largest efforts in evaluating the natural history and clinicopathologic characteristics of nonmetastatic PUC. This is also the largest series examining the response of PUC to preoperative platinum-based chemotherapy and the first assessment of response of advanced PUC to ICIs. For clinically resectable PUC, administration of cisplatin-based chemotherapy was associated with lower pathologic compete response and pathologic downstaging rates than UC NOS. Although many patients receive neoadjuvant chemotherapy, survival among patients with PUC was significantly lower than for patients with UC NOS, even among pathologic responders. Even after preoperative chemotherapy, a large proportion of PUCs were found to be unresectable at the time of surgery. Conversely, response rates and clinical outcomes with ICIs in advanced disease were not dissimilar to reported studies in urothelial cancers in general.10,11,12,13
The unique pattern of PUC dissemination contributes to the under-appreciation of disease extent. In our series, extravesical disease was prevalent among patients who underwent cystectomy first, and cystectomy was aborted due to extensive and fixed disease in 18–28% of patients who underwent preoperative chemotherapy, despite the absence of radiographic progression after systemic therapy. This is consistent with prior reports indicating that PUC is characterised by sheet-like peritoneal dissemination26 and is associated with a higher probability of margin involvement.3,5 In accordance with other published series,27 we reported a high number of recurrences, which were frequently in the peritoneum.
PUC might also be associated with reduced chemosensitivity, as we observed decreased pathologic response and survival than in UC NOS. A post hoc analysis from the AUO-AB05/95 adjuvant trial28 and other retrospective series27,29 did not show a survival advantage for preoperative platinum-based chemotherapy over cystectomy alone. In a recently reported series of 26 PUC cases, 2 pathologic complete responses (10%) and 1 residual non-muscle invasive disease (5%) were noted, an observation consistent with our findings.30 We also observed a low rate of genomic alterations in DDR genes, including ERCC2 and ATM, which have been reported to be associated with response to platinum-based chemotherapy.22,23,29,31,32
Treatment with anti-PD1/PDL1 ICI was associated with durable benefit in a third of patients in our series. Our observed response rate was comparable to ICI data from trials of patients with metastatic UC,10,33 but contrasts with a series of 13 patients with advanced PUC treated with ICI who had poor survival.34 More recently, an interim analysis of a Phase 2 trial of rare genitourinary cancers reported promising activity with ipilimumab and nivolumab, although only one PUC case was enrolled and responded to treatment at the time of reporting.35
Our genomic analysis confirmed the high rate of truncating CDH1 alterations in PUC and further characterised other genes that are frequently altered in PUC. The current series represents the largest cohort of genomically characterised PUC and is independent from our previous analysis, which reported truncating CDH1 mutations in 26/31 (84%) cases.4 Compared with published UC NOS sequencing data, we observed higher rates of alterations in TP53 and RB1 and lower rates of alterations in FGFR3 and DDR genes, which is consistent with other PUC series.4,36
Our study was limited by its size, single institutional, and retrospective nature, with patients treated over a 20-year period. This is unfortunately inevitable due to the rarity of PUC. During this 17-year period, different histomorphological definitions existed; these were updated and consolidated recently.1 To ensure that all cases that were included in our study were PUC, a detailed histopathologic review was performed on each case to confirm the diagnosis.
In summary, in our series of pathologically verified PUC, preoperative chemotherapy response and clinical outcomes were poor relative to UC NOS, indicating that therapeutic strategies other than platinum-based chemotherapy are urgently needed. Genomic analyses have not identified potential therapeutic targets. We found a higher TMB in our cohort; this observation, in addition to PDL1 being expressed in 21–36% of cases, suggests a potential susceptibility to anti-PD1/PDL1 checkpoint blockade.37 In fact, in our small cohort of patients with advanced PUC, durable responses were observed in one-third of patients, supporting further investigations in the treatment of PUC with ICI. Indeed, the aforementioned Phase 2 trial of ipilimumab and nivolumab is still actively enrolling (NCT03333616). In fact, the Alliance A031702 Phase 2 trial of nivolumab, ipilimumab plus cabozantinib is a basket trial for rare genitourinary cancers, and it includes an arm for PUC (NCT03866382). Improved awareness of PUC as an aggressive disease entity is needed to ensure appropriate case selection in order to identify and refine therapeutic strategies for patients with PUC.
Change history
10 February 2022
A Correction to this paper has been published: https://doi.org/10.1038/s41416-022-01721-w
References
Humphrey, P. A., Moch, H., Cubilla, A. L., Ulbright, T. M. & Reuter, V. E. The 2016 WHO Classification of Tumours of the Urinary System and Male Genital Organs-Part B: Prostate and Bladder Tumours. Eur. Urol. 70, 106–119 (2016).
Nigwekar, P., Tamboli, P., Amin, M. B., Osunkoya, A. O., Ben-Dor, D. & Amin, M. B. Plasmacytoid urothelial carcinoma: detailed analysis of morphology with clinicopathologic correlation in 17 cases. Am. J. Surg. Pathol. 33, 417–424 (2009).
Kaimakliotis, H. Z., Monn, M. F., Cheng, L., Masterson, T. A., Cary, K. C., Pedrosa, J. A. et al. Plasmacytoid bladder cancer: variant histology with aggressive behavior and a new mode of invasion along fascial planes. Urology 83, 1112–1116 (2014).
Al-Ahmadie, H. A., Iyer, G., Lee, B. H., Scott, S. N., Mehra, R., Bagrodia, A. et al. Frequent somatic CDH1 loss-of-function mutations in plasmacytoid variant bladder cancer. Nat. Genet 48, 356–358 (2016).
Cockerill, P. A., Cheville, J. C., Boorjian, S. A., Blackburne, A., Thapa, P., Tarrell, R. F. et al. Outcomes following radical cystectomy for plasmacytoid urothelial carcinoma: defining the need for improved local cancer control. Urology 102, 143–147 (2017).
Li, Q., Assel, M., Benfante, N. E., Pietzak, E. J., Herr, H. W., Donat, M. et al. The impact of plasmacytoid variant histology on the survival of patients with urothelial carcinoma of bladder after radical cystectomy. Eur. Urol. Focus 5, 104–108 (2019).
Grossman, H. B., Natale, R. B., Tangen, C. M., Speights, V. O., Vogelzang, N. J., Trump, D. L. et al. Neoadjuvant chemotherapy plus cystectomy compared with cystectomy alone for locally advanced bladder cancer. N. Engl. J. Med. 349, 859–866 (2003).
Scosyrev, E., Ely, B. W., Messing, E. M., Speights, V. O., Grossman, H. B., Wood, D. P. et al. Do mixed histological features affect survival benefit from neoadjuvant platinum-based combination chemotherapy in patients with locally advanced bladder cancer? A secondary analysis of Southwest Oncology Group-Directed Intergroup Study (S8710). BJU Int 108, 693–699 (2011).
Kaimakliotis, H. Z., Monn, M. F., Cho, J. S., Pedrosa, J. A., Hahn, N. M., Albany, C. et al. Neoadjuvant chemotherapy in urothelial bladder cancer: impact of regimen and variant histology. Future Oncol. 12, 1795–1804 (2016).
Bellmunt, J., de Wit, R., Vaughn, D. J., Fradet, Y., Lee, J. L., Fong, L. et al. Pembrolizumab as second-line therapy for advanced urothelial carcinoma. N. Engl. J. Med. 376, 1015–1026 (2017).
Balar, A. V., Galsky, M. D., Rosenberg, J. E., Powles, T., Petrylak, D. P., Bellmunt, J. et al. Atezolizumab as first-line treatment in cisplatin-ineligible patients with locally advanced and metastatic urothelial carcinoma: a single-arm, multicentre, phase 2 trial. Lancet 389, 67–76 (2017).
Powles, T., Durán, I., van der Heijden, M. S., Loriot, Y., Vogelzang, N. J., De Giorgi, U. et al. Atezolizumab versus chemotherapy in patients with platinum-treated locally advanced or metastatic urothelial carcinoma (IMvigor211): a multicentre, open-label, phase 3 randomised controlled trial. Lancet 391, 748–757 (2018).
Sharma, P., Siefker-Radtke, A., de Braud, F., Basso, U., Calvo, E., Bono, P. et al. Nivolumab alone and with ipilimumab in previously treated metastatic urothelial carcinoma: CheckMate 032 nivolumab 1 mg/kg plus ipilimumab 3 mg/kg expansion cohort results. J. Clin. Oncol. 37, 1608–1616 (2019).
Necchi, A., Anichini, A., Raggi, D., Briganti, A., Massa, S., Luciano, R. et al. Pembrolizumab as neoadjuvant therapy before radical cystectomy in patients with muscle-invasive urothelial bladder carcinoma (PURE-01): an open-label, single-arm, phase II study. J. Clin. Oncol. 36, 3353–3360 (2018).
Powles, T., Kockx, M., Rodriguez-Vida, A., Duran, I., Crabb, S. J., Van Der Heijden, M. S. et al. Clinical efficacy and biomarker analysis of neoadjuvant atezolizumab in operable urothelial carcinoma in the ABACUS trial. Nat. Med. 25, 1706–1714 (2019).
Tully, C. M., Bochner, B. H., Dalbagni, G., Zabor, E. C., Herr, H. W., Donat, S. M. et al. Gemcitabine-cisplatin (GC) plus radical cystectomy-pelvic lymph node dissection (RC-PLND) for patients (pts) with muscle-invasive bladder cancer (MIBC): assessing impacts of neoadjuvant chemotherapy (NAC) and the PLND. J. Clin. Oncol. 32(4_suppl), 355 (2014).
Cheng, D. T., Mitchell, T. N., Zehir, A., Shah, R. H., Benayed, R., Syed, A. et al. Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT): a hybridization capture-based next-generation sequencing clinical assay for solid tumor molecular oncology. J. Mol. Diagn. 17, 251–264 (2015).
Zehir, A., Benayed, R., Shah, R. H., Syed, A., Middha, S., Kim, H. R. et al. Mutational landscape of metastatic cancer revealed from prospective clinical sequencing of 10,000 patients. Nat. Med. 23, 703–713 (2017).
Tate, J. G., Bamford, S., Jubb, H. C., Sondka, Z., Beare, D. M., Bindal, N. et al. COSMIC: the Catalogue Of Somatic Mutations In Cancer. Nucleic Acids Res. 47(D1), D941–D947 (2019).
Chang, M. T., Asthana, S., Gao, S. P., Lee, B. H., Chapman, J. S., Kandoth, C. et al. Identifying recurrent mutations in cancer reveals widespread lineage diversity and mutational specificity. Nat. Biotechnol. 34, 155–163 (2016).
Chakravarty D., Gao J., Phillips S. M., Kundra R., Zhang H., Wang J. et al. OncoKB: a precision oncology knowledge base. JCO Precis. Oncol. https://doi.org/10.1200/po.17.00011 (2017).
Iyer, G., Balar, A. V., Milowsky, M. I., Bochner, B. H., Dalbagni, G., Donat, S. M. et al. Multicenter prospective phase II trial of neoadjuvant dose-dense gemcitabine plus cisplatin in patients with muscle-invasive bladder cancer. J. Clin. Oncol. 36, 1949–1956 (2018).
Teo, M. Y., Bambury, R. M., Zabor, E. C., Jordan, E., Al-Ahmadie, H., Boyd, M. E. et al. DNA damage response and repair gene alterations are associated with improved survival in patients with platinum-treated advanced urothelial carcinoma. Clin. Cancer Res 23, 3610–3618 (2017).
Robertson, A. G., Kim, J., Al-Ahmadie, H., Bellmunt, J., Guo, G., Cherniack, A. D. et al. Comprehensive molecular characterization of muscle-invasive bladder cancer. Cell 171, 540.e5–556.e5 (2017).
Robertson, A. G., Kim, J., Al-Ahmadie, H., Bellmunt, J., Guo, G., Cherniack, A. D. et al. Comprehensive molecular characterization of muscle-invasive bladder cancer. Cell 174, 1033 (2018).
Chung, A. D., Schieda, N., Flood, T. A., Cagiannos, I., Mai, K. T., Malone, S. et al. Plasmacytoid urothelial carcinoma (PUC): imaging features with histopathological correlation. Can. Urol. Assoc. J. 11, E50–E57 (2017).
Dayyani, F., Czerniak, B. A., Sircar, K., Munsell, M. F., Millikan, R. E., Dinney, C. P. et al. Plasmacytoid urothelial carcinoma, a chemosensitive cancer with poor prognosis, and peritoneal carcinomatosis. J. Urol. 189, 1656–1661 (2013).
Keck, B., Wach, S., Stoehr, R., Kunath, F., Bertz, S., Lehmann, J. et al. Plasmacytoid variant of bladder cancer defines patients with poor prognosis if treated with cystectomy and adjuvant cisplatin-based chemotherapy. BMC Cancer 13, 71 (2013).
Diamantopoulos, L. N., Khaki, A. R., Vakar-Lopez, F., Tretiakova, M. S., Gore, J. L., Schade, G. R. et al. Patient characteristics, treatment patterns, outcomes and prognostic factors in plasmacytoid urothelial carcinoma. J. Clin. Oncol. 37, e16007 (2019).
Diamantopoulos, L. N., Khaki, A. R., Grivas, P., Gore, J. L., Schade, G. R., Hsieh, A. C. et al. Plasmacytoid urothelial carcinoma: response to chemotherapy and oncologic outcomes. Bladder Cancer 6, 71–81 (2020).
Liu, D., Plimack, E. R., Hoffman-Censits, J., Garraway, L. A., Bellmunt, J., Van Allen, E. et al. Clinical validation of chemotherapy response biomarker ERCC2 in muscle-invasive urothelial bladder carcinoma. JAMA Oncol. 2, 1094–1096 (2016).
Van Allen, E. M., Mouw, K. W., Kim, P., Iyer, G., Wagle, N., Al-Ahmadie, H. et al. Somatic ERCC2 mutations correlate with cisplatin sensitivity in muscle-invasive urothelial carcinoma. Cancer Discov. 4, 1140–1153 (2014).
Rosenberg, J. E., Hoffman-Censits, J., Powles, T., van der Heijden, M. S., Balar, A. V., Necchi, A. et al. Atezolizumab in patients with locally advanced and metastatic urothelial carcinoma who have progressed following treatment with platinum-based chemotherapy: a single-arm, multicentre, phase 2 trial. Lancet 387, 1909–1920 (2016).
Andrews, E., Curran, C., Grivas, P., Diamantopoulos, L. N., Drakaki, A., Jain, R. K. et al. Outcomes of advanced plasmacytoid urothelial carcinoma receiving systemic therapy. J. Clin. Oncol. 38, 580 (2020).
McGregor, B. A., Campbell, M. T., Xie, W., Farah, S., Bilen, M. A., Sonpavde, G. et al. Phase II study of nivolumab and ipilimumab for advanced rare genitourinary cancers. J. Clin. Oncol. 38(15_suppl), 5018–5018 (2020).
Ramamurthy, C., Arguello, D., Anari, F., Ghatalia, P., Zibelman, M. R., Plimack, E. R. et al. Molecular profiling of aggressive variant urothelial carcinoma. J. Clin. Oncol. 37(7_suppl), 378 (2019).
Reis, H., Serrette, R., Posada, J., Lu, V., Chen, Y. B., Gopalan, A. et al. PD-L1 expression in urothelial carcinoma with predominant or pure variant histology: concordance among 3 commonly used and commercially available antibodies. Am. J. Surg. Pathol. 43, 920–927 (2019).
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Sara DiNapoli provided editorial support for this manuscript.
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Contributions
M.Y.T. conceptualised and designed the study, acquired data, and drafted the manuscript. H.A.-A. conceptualised and designed the study, acquired data, and analysed and interpreted the data. K.S. drafted the manuscript and performed statistical analysis. C.T. acquired data. A.M.R. provided technical support and revised the manuscript. E.P., D.B.S., E.C., H.H., T.D., S.M.D., G.D., B.H.B., S.F., and D.F.B. acquired data and revised the manuscript. S.T. and V.R. acquired data. G.I. conceptualised the study, acquired data and revised the manuscript. I.O. performed statistical analysis. J.E.R. drafted the manuscript and supervised the study.
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This study was approved by the Memorial Sloan Kettering Cancer Center Institutional Review Board (#17–214). The need for informed consent was waived by the institutional review board. This study was performed in accordance with the Declaration of Helsinki.
Data availability
Anonymised data will be provided upon request.
Competing interests
MYT has received Research Support from Bristol Myers Squibb, Clovis and Pharmacyclics; DBS has consulted with/received honoraria from Pfizer, Loxo Oncology, Lilly Oncology, BridgeBio, Vividion Therapeutics, Scorpion Therapeutics and Illumina; SAF has received research support from AstraZeneca, Genentech/Roche, is a consultant/advisory board member for Merck, and owns stock in Urogen, Allogene Therapeutics, Neogene Therapeutics, Kronos Bio, and Inconovir; DFB has received research funding from Novartis, has received personal fees from Merck Sharp & Dohme, Eisai, Fidia Farmaceutici S.p.A., Lilly, and UroGen Pharma; and has received grants and personal fees from Bristol‐Myers Squibb, Roche/Genentech, and Novartis; and grants from Dendreon; GVI has received personal fees from Mirati Therapeutics and Janssen and research support from Novartis; JER has consulted for AstraZeneca, Bayer, Merck, BMS, Roche, Genentech, Seattle Genetics, Astellas, Boehringher Ingelheim, GSK, Mirati, Janssen, Lilly, and Pfizer. He has also received funding for clinical trials from Roche/Genentech, AstraZeneca, Bayer, Seattle Genetics and Astellas.
Funding information
This work was supported in part by the National Cancer Institute at the National Institutes of Health [P30 CA008748 to Memorial Sloan Kettering Cancer Center].
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Teo, M.Y., Al-Ahmadie, H., Seier, K. et al. Natural history, response to systemic therapy, and genomic landscape of plasmacytoid urothelial carcinoma. Br J Cancer 124, 1214–1221 (2021). https://doi.org/10.1038/s41416-020-01244-2
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DOI: https://doi.org/10.1038/s41416-020-01244-2
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