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Evaluation of diagnostic performance of metagenomic next-generation sequencing when applied in patients after allogeneic hematopoietic stem cell transplant

Bone Marrow Transplantation volume 56, pages 2860–2861 (2021)Cite this article

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Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is widely used to bring patients with various hematologic malignancies cure by rebuilding their immune system [1]. Unfortunately, Patients after allo-HSCT are under the threat of infections because of immunodeficiency [2, 3]. In clinical practice, there are a range of conventional tests including culture, smear microscopy, plasma 1, 3-β-D-glucan (G)/galactomannan (GM) tests, C-reactive protein (CRP) test, procalcitonin (PCT) test and so on applied to identify pathogens [4, 5], while they are time-consuming and the preceding information about patients are significant. A completely new pathogen detection method is urgently required. Metagenomic next-generation sequencing (mNGS) is a high throughput technology that can detect all the organisms of one sample [6,7,8]. However, reports evaluating the ability of mNGS to detect pathogens from patients after allo-HSCT remain scarce [9, 10].

We retrospectively reviewed 149 cases suspected of infection after allo-HSCT in 3 years at the First Affiliated Hospital of Soochow University in Suzhou, China, between April 2018 and January 2021 and all the 149 cases were analysed and categorized into 2 groups-infection groups (IG) and non-infection groups (NIG) on the basis of final clinical diagnosis.

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References

  1. Ray P, De A, Yaghoubi S, Khanna A. Application of adult stem cells in medicine. Stem Cells Int. 2015;2015:258313.

    Article  Google Scholar 

  2. Ogonek J, Kralj Juric M, Ghimire S, Varanasi PR, Holler E, Greinix H, et al. Immune reconstitution after allogeneic hematopoietic stem cell transplantation. Front Immunol. 2016;7:507.

    Article  Google Scholar 

  3. Styczyński J, Tridello G, Koster L, Iacobelli S, van Biezen A, van der Werf S, et al. Infectious Diseases Working Party EBMT. Death after hematopoietic stem cell transplantation: changes over calendar year time, infections and associated factors. Bone Marrow Transpl. 2020;55:126–36.

    Article  Google Scholar 

  4. McKenzie R, Reimer LG. Effect of antimicrobials on blood cultures in endocarditis. Diagn Microbiol Infect Dis. 1987;8:165–72.

    Article  CAS  Google Scholar 

  5. Li L, Xu L, Zhu R, Song J, Wang X. Effect of prior receipt of antibiotics on the pathogen distribution: a retrospective observational cohort study on 27,792 patients. BMC Infect Dis. 2020;20:8.

    Article  CAS  Google Scholar 

  6. Palacios G, Druce J, Du L, Tran T, Birch C, Briese T, et al. A new arenavirus in a cluster of fatal transplant-associated diseases. N. Engl J Med. 2008;358:991–8.

    Article  CAS  Google Scholar 

  7. Xu B, Liu L, Huang X, Ma H, Zhang Y, Du Y, et al. Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: discovery of a new bunyavirus. PLoS Pathog. 2011;7:e1002369.

    Article  CAS  Google Scholar 

  8. Simner PJ, Miller S, Carroll KC. Understanding the Promises and Hurdles of Metagenomic Next-Generation Sequencing as a Diagnostic Tool for Infectious Diseases. Clin Infect Dis. 2018;66:778–88.

    Article  CAS  Google Scholar 

  9. Niles DT, Wijetunge DSS, Palazzi DL, Singh IR, Revell PA. Plasma metagenomic next-generation sequencing assay for identifying pathogens: a retrospective review of test utilization in a large children’s hospital. J Clin Microbiol. 2020;58:e00794–20.

    Article  CAS  Google Scholar 

  10. Hogan CA, Yang S, Garner OB, Green DA, Gomez CA, Dien Bard J, et al. Clinical impact of metagenomic next-generation sequencing of plasma cell-free DNA for the diagnosis of infectious diseases: a multicenter retrospective cohort study. Clin Infect Dis. 2021;72:239–45.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China

    Yanjun Wu, Ye Zhao, Tingjing Wang, Jing Xia, Depei Wu & Feng Chen

  2. Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China

    Yanjun Wu, Ye Zhao, Tingjing Wang, Jing Xia, Depei Wu & Feng Chen

  3. Key Laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou, China

    Yanjun Wu, Ye Zhao, Tingjing Wang, Jing Xia, Depei Wu & Feng Chen

Authors
  1. Yanjun Wu
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  2. Ye Zhao
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  3. Tingjing Wang
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  4. Jing Xia
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  5. Depei Wu
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Contributions

YW, DW, and FC conceived the study and interpreted the data; YW, and FC wrote the paper; YW, YZ, and TW performed the statistical analyses; JX, DW, and FC reviewed the paper and contributed to the final draft.

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Correspondence to Depei Wu or Feng Chen.

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Wu, Y., Zhao, Y., Wang, T. et al. Evaluation of diagnostic performance of metagenomic next-generation sequencing when applied in patients after allogeneic hematopoietic stem cell transplant. Bone Marrow Transplant 56, 2860–2861 (2021). https://doi.org/10.1038/s41409-021-01423-6

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  • DOI: https://doi.org/10.1038/s41409-021-01423-6

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