TO THE EDITOR:
Myelodysplastic syndrome (MDS) with del(5q) is a subcategory of MDS without excess blasts (<5% in bone marrow and 2% peripheral blood) and is characterized by the presence of an often isolated del5q cytogenetic abnormality. In addition, the diagnosis according to the International Consensus Classification (ICC) allows for the presence of one additional cytogenetic abnormality, exclusive of -7/del(7q), while requiring the absence of “multi-hit” TP53 mutation [1]. Patients with MDS-del(5q) also harbor other somatic mutations, with the two most frequent being TP53 (~20% incidence at diagnosis) and SF3B1 (~18%) [2]. These two mutations display significant clustering and are more prevalent in leukemic phase disease (~50% incidence), suggesting pathogenetic contribution to leukemic progression [2]. Furthermore, TP53 variant allele frequency (VAF) > 22% has been shown to portend inferior overall and leukemia-free survival [2] and, along with therapy-related disease, was recently identified as the most prominent risk factor for survival in MDS-del(5q) [3]. The current standard of care in MDS-del(5q) is lenalidomide monotherapy for the alleviation of anemia [4, 5]. The emergence of TP53 mutations during lenalidomide therapy of patients with MDS-del(5q) has previously been published [6,7,8]. In the current report, we extend these observations in an unselected cohort of 10 Mayo Clinic patients with MDS-del(5q) in whom next-generation sequencing (NGS) information was available before and after treatment with lenalidomide. The study was conducted under institutional review board-approved minimum risk protocols that allowed retrospective collection and analysis of patient history. Diagnosis of MDS-del(5q) and therapy-related qualification was according to ICC criteria [1]. Patient selection was based on the availability of NGS-derived mutation information before starting treatment with lenalidomide and at least one additional NGS analysis during treatment with lenalidomide.
Table 1 illustrates the mutational landscape in each of the 10 study patients (median age 75 years, range 65–79; 6 males), both before the start of treatment (1st NGS) with lenalidomide and a second-time point (2nd NGS) during treatment with lenalidomide; therapy-related disease was documented in 2 patients (patients 7 and 8). Baseline (1st) NGS study was performed before initiation of treatment with lenalidomide in all but one patient who was studied within 6 weeks of treatment with lenalidomide (Table 1; patient 2). The median time from initial diagnosis of MDS-del(5q) to 1st NGS study was 16 months (range 0–80). Mutation profiling at baseline (1st NGS; prior to lenalidomide therapy) disclosed one (10%) patient with monoallelic TP53 mutation (VAF 19%; patient 4; Table 1) and two other patients (patients 1 and 2) with SF3B1 mutation (VAF 8% and 14%); one of the latter SF3B1-mutated patients (patient 2) also harbored an ASXL1 mutation (VAF 37%). The second NGS study during lenalidomide therapy was performed in all 10 patients at a median of 27 months (range 10-58) from the time of the first NGS study (Table 1). New TP53 mutations emerged in 3 (33%) of 9 patients in whom the mutation was not detected at baseline (patients 1, 2, and 3; Table 1); the mutations were monoallelic (VAF 31%) in patient 1 (19 months from the 1st NGS study) and biallelic in the other two, with VAF of 22%/12% (patient 2; 49 months from the 1st NGS study) in one and 9%/3% (patient 3; 58 months from the 1st NGS study) in the other. In one patient (patient 4) harboring a TP53 mutation at baseline (1st NGS study), TP53 VAF decreased from 19% to 6% at 13-month interval (Table 1). Among the 3 patients with treatment-emergent TP53 mutations, two were known to harbor SF3B1 mutations at baseline (patient 1 with VAF 14% and patient 2 with VAF 8%) with patient 2 losing the SF3B1 mutation at 49 months interval while SF3B1 VAF increased in patient 1 from 14% to 32% at 19 months interval. Another patient (patient 9) acquired a new SF3B1 mutation (VAF 5%), during the 2nd NGS study at 25 months interval (Table 1); the latter patient also acquired CEBPA (VAF 26%) and WT1 (VAF 2%) mutations at the same time (Table 1). Additional lenalidomide treatment-emergent mutations included ASXL1 (VAF 4%) and IDH1 (VAF 4%) in patient 3 at 58 months interval and RUNX1 (VAF 2%) in patient 1 at 19 months interval; all three patients were those with treatment-emergent TP53 mutations (Table 1).
Cytogenetic information at the time of diagnosis showed sole del(5q) in 8 patients and one other additional cytogenetic abnormality in 2 patients; patient 3 with del(17)(q25) in 14 of 15 metaphases and patient 10 with del(12)(p11.2p13) in 8 of 11 metaphases. Cytogenetic information at the time of the 2nd NGS study was also available in 9 patients and revealed no changes in 7 cases and disapperance of del(5q) in 2 patients (patients 2 and 8); both patients with lenalidomide treatment-associated disapperance of del(5q) started with only two abnormal metaphases at baseline. Transfusion-dependent anemia was documented in 1 patient at the time of the 1st NGS study (patient 1) and remained transfusion-dependent at the time of the 2nd NGS study but showed a steep decline in platelet count (174 to 53 × 10(9)/L; Table 1); the particular patient had responded to lenalidomide therapy and had become transfusion-independent in-between the 1st and 2nd NGS studies but had lost this response at the time of the 2nd NGS study, concurrent with the emergence of a new TP53 mutation (VAF 31%; Table 1). Of note, patient 1 was recently described in a case report where salvage therapy with attenuated dose decitabine was shown to be successful in eradicating the TP53 mutation and resumption of a transfusion-independent state [8]. Hemoglobin levels in the other 2 patients with treatment-emergent TP53 mutations either improved (patient 2) or worsened (patient 3); the latter patient had also acquired new ASXL1 (VAF 4%) and IDH1 (VAF 4%) mutations (Table 1). Hemoglobin levels in four of the remaining 7 patients showed a decline, including patient 8 who acquired new SF3B1 (5%), CEBPA (26%), and WT1 (VAF 2%) mutations (Tables 1 and 2) or either an improvement or no change in 3 cases (Table 2). At last follow-up, all three patients with treatment-emergent TP53 mutations were alive at 10 (patient 1), 52 (patient 2), and 44 (patient 3) months post-lenalidomide therapy; of note, the TP53 mutations in the latter two patients were biallelic. None of the three patients with treatment-emergent TP53 mutations were previously exposed to chemotherapy or radiotherapy and none experienced leukemic transformation; the latter was documented in patients 6 and 10, neither of whom displayed any mutation at baseline or during treatment with lenalidomide (Table 1).
The current report confirms previously published observations regarding treatment-emergent TP53 mutations in patients with MDS-del(5q) receiving lenalidomide therapy [9,10,11]. In one of these reports, Lode et al., described 24 patients with MDS-del(5q) who had recieved lenalidomide therapy with 75% erythroid response and 21% complete cytogenetic response [7]; peripheral blood or bone marrow samples from these patients were retrospectively screened for TP53 mutations with 6 (25%) patients expressing the mutation at diagnosis and another 9 (38%) during follow-up, of whom, one also manifested a new RUNX1 mutation, as was the case in the current report. The particular study showed appearance of TP53 mutations in 9 (50%) of 18 patients in whom the mutation was not detected at diagnosis and the authors were able to show correlation between TP53 clonal evolution and disease progression [7]. Interestingly, in a more recent report of 416 patients with therapy-related myeloid neoplasms, the authors described an association between TP53 mutations and prior treatment with thalidomide analogs, specifically lenalidomide, and the authors were able to provide experimental evidence for lenalidomide-induced selective advantage for TP53 mutant clones [6]. Taken together, these observations suggest lenalidomide-enhanced expansion of pre-existing mutant TP53 clones that might be less sensitive to lenalidomide-induced clonal suppression in MDS-del(5q). However, none of the aforementioned studies were controlled and do not necessarily prove cause and effect and, instead, the particular phenomenon might represent clonal evolution as an intrinsic disease biology that may or may not be influenced by specific therapy. The latter impression is consistent with the observations from the current study, which suggest lenalidomide treatment-emergent mutations might not be restricted to TP53 but also involve other genes.
Current practice in the real-world setting, in regard to patients with MDS-del(5q), is heterogeneous, in terms of both treatment initiation with lenalidomide and disease monitoring [12, 13]. The observations from the current study underline the need for monitoring of mutations both at diagnosis (preferably using highly sensitive assays) and during the disease course, in patients with MDS-del(5q), regardless of specific treatment. Actionable events include the presence of TP53 VAF ≥ 22%, which requires intervention with allogeneic stem cell transplant [14], and utilization of alternative therapy, such as hypomethylating agents [6], which might be active in the absence of complex karyotype [15].
Data availability
Data might be requested by email to the corresponding author.
References
Arber DA, Orazi A, Hasserjian RP, Borowitz MJ, Calvo KR, Kvasnicka HM, et al. International Consensus Classification of Myeloid Neoplasms and Acute Leukemias: integrating morphologic, clinical, and genomic data. Blood. 2022;140:1200–28. https://doi.org/10.1182/blood.2022015850.
Fleti F, Chan O, Singh A, Abdelmagid MG, Al-Kali A, Elliott MA, et al. TP53 mutations and variant allele frequency in myelodysplastic syndromes with del(5q): A Mayo-Moffitt study of 156 informative cases. Am J Hematol. 2023;98:E76–E79. https://doi.org/10.1002/ajh.26845.
Tefferi A, Fleti F, Chan O, Al Ali NH, Al-Kali A, Begna KH, et al. TP53 variant allele frequency and therapy-related setting independently predict survival in myelodysplastic syndromes with del(5q). Br J Haematol. 2023. https://doi.org/10.1111/bjh.19247.
Patnaik MM, Lasho TL, Finke CM, Gangat N, Caramazza D, Holtan SG, et al. WHO-defined ‘myelodysplastic syndrome with isolated del(5q)’ in 88 consecutive patients: survival data, leukemic transformation rates and prevalence of JAK2, MPL and IDH mutations. Leukemia. 2010;24:1283–9. https://doi.org/10.1038/leu.2010.105.
List A, Kurtin S, Roe DJ, Buresh A, Mahadevan D, Fuchs D, et al. Efficacy of lenalidomide in myelodysplastic syndromes. N. Engl J Med. 2005;352:549–57. https://doi.org/10.1056/NEJMoa041668.
Sperling AS, Guerra VA, Kennedy JA, Yan Y, Hsu JI, Wang F, et al. Lenalidomide promotes the development of TP53-mutated therapy-related myeloid neoplasms. Blood. 2022;140:1753–63. https://doi.org/10.1182/blood.2021014956.
Lode L, Menard A, Flet L, Richebourg S, Loirat M, Eveillard M, et al. Emergence and evolution of TP53 mutations are key features of disease progression in myelodysplastic patients with lower-risk del(5q) treated with lenalidomide. Haematologica. 2018;103:e143–e146. https://doi.org/10.3324/haematol.2017.181404.
Gangat N, Bellam N, Reichard K, Tefferi A Emergence of TP53 mutation during lenalidomide therapy of myelodysplastic syndrome with del(5q) and its subsequent disappearance following salvage therapy with decitabine. Haematologica. 2023. https://doi.org/10.3324/haematol.2023.284547.
Jadersten M, Saft L, Pellagatti A, Gohring G, Wainscoat JS, Boultwood J, et al. Clonal heterogeneity in the 5q- syndrome: p53 expressing progenitors prevail during lenalidomide treatment and expand at disease progression. Haematologica. 2009;94:1762–6. https://doi.org/10.3324/haematol.2009.011528.
Jadersten M, Saft L, Smith A, Kulasekararaj A, Pomplun S, Gohring G, et al. TP53 mutations in low-risk myelodysplastic syndromes with del(5q) predict disease progression. J Clin Oncol. 2011;29:1971–9. https://doi.org/10.1200/JCO.2010.31.8576.
Mossner M, Jann JC, Nowak D, Platzbecker U, Giagounidis A, Gotze K, et al. Prevalence, clonal dynamics and clinical impact of TP53 mutations in patients with myelodysplastic syndrome with isolated deletion (5q) treated with lenalidomide: results from a prospective multicenter study of the German MDS Study Group (GMDS). Leukemia. 2016;30:1956–9. https://doi.org/10.1038/leu.2016.111.
Rufer A, Angermann H, Benz R, Bonadies N, Calderoni A, Cantoni N, et al. Real-world Data From the Swiss Lenalidomide in MDS del(5q) (SLIM)-Registry Identify New Chances and Challenges in Lenalidomide Treatment of Patients With MDS del(5q). Hemasphere. 2022;6:e741. https://doi.org/10.1097/HS9.0000000000000741.
Gurnari C, Piciocchi A, Soddu S, Bonanni F, Scalzulli E, Niscola P, et al. Myelodysplastic syndromes with del(5q): A real-life study of determinants of long-term outcomes and response to lenalidomide. Blood Cancer J. 2022;12:132. https://doi.org/10.1038/s41408-022-00724-3.
Garderet L, Ziagkos D, van Biezen A, Iacobelli S, Finke J, Maertens J, et al. Allogeneic stem cell transplantation for myelodysplastic syndrome patients with a 5q deletion. Biol Blood Marrow Transpl. 2018;24:507–13. https://doi.org/10.1016/j.bbmt.2017.11.017.
Pasca S, Haldar SD, Ambinder A, Webster JA, Jain T, Dalton WB et al. Outcome heterogeneity of TP53-mutated myeloid neoplasms and the role of allogeneic hematopoietic cell transplantation. Haematologica 2023. https://doi.org/10.3324/haematol.2023.283886.
Author information
Authors and Affiliations
Contributions
AT wrote the paper, performed the statistical analysis and contributed patients; KR provided pathology and molecular expertise; NG participated in study design and data abstraction; MA participated in data abstraction; all authors reviewed and approved the manuscript.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Abdallah, M., Reichard, K., Gangat, N. et al. Treatment-emergent mutations in myelodysplastic syndrome with del(5q) – lenalidomide related or disease-intrinsic clonal evolution?. Blood Cancer J. 14, 49 (2024). https://doi.org/10.1038/s41408-024-01027-5
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41408-024-01027-5
This article is cited by
-
Lenalidomide
Reactions Weekly (2024)