Abstract
Our previous study showed that chronic treatment with tumor necrosis factor-α (TNF-α) decreased cAMP concentration in fibroblast-like synoviocytes (FLSs) of collagen-induced arthritis (CIA) rats. In this study we investigated how TNF-α impairs cAMP homeostasis, particularly clarifying the potential downstream molecules of TNF-α and prostaglandin receptor 4 (EP4) signaling that would interact with each other. Using a cAMP FRET biosensor PM-ICUE3, we demonstrated that TNF-α (20 ng/mL) blocked ONO-4819-triggered EP4 signaling, but not Butaprost-triggered EP2 signaling in normal rat FLSs. We showed that TNF-α (0.02–20 ng/mL) dose-dependently reduced EP4 membrane distribution in normal rat FLS. TNF-α significantly increased TNF receptor 2 (TNFR2) expression and stimulated proliferation in human FLS (hFLS) via ecruiting TNF receptor-associated factor 2 (TRAF2) to cell membrane. More interestingly, we revealed that TRAF2 interacted with G protein-coupled receptor kinase (GRK2) in the cytoplasm of primary hFLS and helped to bring GRK2 to cell membrane in response of TNF-α stimulation, the complex of TRAF2 and GRK2 then separated on the membrane, and translocated GRK2 induced the desensitization and internalization of EP4, leading to reduced production of intracellular cAMP. Silencing of TRAF2 by siRNA substantially diminished TRAF2-GRK2 interaction, blocked the translocation of GRK2, and resulted in upregulated expression of membrane EP4 and intracellular cAMP. In CIA rats, administration of paroxetine to inhibit GRK2 effectively improved the symptoms and clinic parameters with significantly reduced joint synovium inflammation and bone destruction. These results elucidate a novel form of cross-talk between TNFR (a cytokine receptor) and EP4 (a typical G protein-coupled receptor) signaling pathways. The interaction between TRAF2 and GRK2 may become a potential new drug target for the treatment of inflammatory diseases.
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Introduction
Fibroblast-like synoviocyte (FLS) hyperplasia is the principle feature and initiator of rheumatoid arthritis (RA). Excessive proliferation of FLSs is triggered by a variety of proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β) and prostaglandin E2 (PGE2) [1]. TNF-α is a major mediator of the inflammatory response and is implicated in the onset of RA [2]. In addition, TNF-α significantly promotes the production of PGE2 in FLSs [1]. Cytokine-mediated induction of PGE2 is the key factor that induces arthralgia both locally and centrally; furthermore, PGE2 was reported to be a major player in exacerbating the proliferation of synoviocytes [3]. These pathological functions of PGE2 clearly illustrate the beneficial effects of selective cyclooxygenase-2 (COX-2) inhibitors and nonsteroidal anti-inflammatory drugs (NSAIDs) in the clinic. Both types of anti-inflammatory drugs exert their functions mainly through inhibiting COX, thus impairing the production of PGE2 [4, 5].
Conversely, a potent immunosuppressive or immunomodulatory effect of PGE2 was reported based on its ability to increase intracellular cAMP levels [6, 7]. PGE2 binds and activates four prostaglandin receptors (EPs), known as EP1, EP2, EP3 and EP4 [8], which are typical G protein-coupled receptors (GPCRs) and are precisely controlled by G protein-coupled receptor kinases (GRKs) and β-arrestins. EP2 and EP4 are linked to stimulatory G protein (Gαs), through which they activate adenylate cyclase (AC) and increase intracellular cyclic adenosine monophosphate (cAMP) levels; EP1 is linked to Gαq, while EP3 couples with inhibitory G protein (Gαi) and reduces cAMP levels [9]. Human FLSs (hFLSs) have constitutive mRNA expression of EP2 and EP4; however, there have been inconsistent reports on EP1 and EP3 mRNA expression [10]. Therefore, EP2 and EP4 are regarded as attractive pharmacological targets for RA treatment. In the inflammatory microenvironment of arthritic joints, abundant PGE2 stimulation leads to increases in intracellular cAMP levels via the activation of EP2 and EP4, thus inhibiting FLS proliferation through the following pathways: (a) activating exchange protein activated by cAMP (EPAC)/Rap1 signaling [11]; (b) inhibiting extracellular signal-regulated kinase (Erk) phosphorylation and the mitogen-activated protein kinase (MAPK) pathway, which controls cell proliferation and differentiation [12]; (c) promoting the inhibitory effect of C1q/TNF-related protein (CTRP) 9 on DNA synthesis [13]; (d) suppressing the activation of mammalian target of rapamycin complex (mTORC) by both PKA and the PKB-MAPK/adenosine monophosphate activated protein kinase (AMPK)-TSC1/2 pathway, thus triggering the dissociation of mTORC and the inactivation of mTOR kinase, which leads to the inhibition of cell proliferation [14]; and e) controlling the transcription of proliferative genes targeted by cAMP element binding (CREB) or the YAP ortholog Yorki [15]. The fundamental underlying reason for opposing functions of PGE2-EP signaling in the inflammatory microenvironment with abnormal increases in the proinflammatory cytokine TNF-α is worth exploring to attain better understanding of the pathogenesis of RA. Moreover, how TNF-α affects EP receptor-mediated signaling remains largely unknown.
TNF-α interacts with two different receptors: TNF receptor 1 (TNFR1) and TNFR2. TNFR family signaling is mediated by tumor necrosis factor receptor-associated factors (TRAFs). To date, six TRAF family proteins (TRAF1-TRAF6) have been identified. All TRAFs share a common sequence of amino acids at their carboxyl termini termed the TRAF domain, which is required for the binding of the cytoplasmic domain [16].
Typically, TNFR signaling is independent of the cAMP pathway, but interestingly, our previous work showed that after TNF-α stimulation, the cAMP concentration in FLSs in collagen-induced arthritis (CIA) rats was decreased [17]. The present study aimed to explore how TNF-α regulated cAMP production in FLSs, particularly by further clarifying the potential downstream molecules of TNF-α and EP4 signaling that interact with each other. This investigation will not only identify a new form of crosstalk between TNF-α and EP4 signaling but also reveal a novel pattern of TNF-α action in inflammatory arthritis.
Materials and methods
Experimental animals
Sprague–Dawley (SD) rats (male, 140 ± 20 g, Certificate No. SCXK (WAN) 2011–002) were obtained from the Animal Department of Anhui Medical University (Hefei, China). All rats were maintained under SPF conditions. The experiments reported here were approved by the Ethics Review Committee for Animal Experimentation of the Institute of Clinical Pharmacology, Anhui Medical University.
Antibodies and reagents
Incomplete Freund’s adjuvant (IFA) and chick CB peptides of type II collagen (CII) were obtained from Chondrex Inc. (Redmond, WA, USA). Recombinant human TNF-α was purchased from Peprotech Inc. (Rocky Hill, NJ, USA). Indometacin (Cat. No. 17378) was obtained from Sigma-Aldrich (Saint Louis, MO, USA). TNF-R1 (H-5) (Cat#: sc-8436), TNF-R2 (D-2) (Cat#: sc-8041), EP4 (Cat#: sc-55596), GRK2 (C-9) (Cat#: sc-13143), TRAF2 (H-10) (Cat#: sc-7346), TNFR1 siRNA (siTNFR1) (Cat. No. sc-29507), TNFR2 siRNA (siTNFR2) (Cat. No. sc-36689), and control siRNA (Cat. No. sc-37007) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Protein A+G (Cat. No. P2012) agarose was obtained from Beyotime (Shanghai, China). Cy3-conjugated Goat anti-mouse IgG (H+L) (Cat#: 115–167–003), Alexa Fluor 488 AffiniPure goat anti-mouse IgG (H+L) (Cat#: 115–545–062), Alexa Fluor Rhodamine Red AffiniPure donkey anti-goat IgG (H+L) (Cat#: 705–295–003) and Alexa Fluor 488 AffiniPure goat anti-rabbit IgG (H+L) (Cat#: 111–005–003) antibodies were purchased from Jackson ImmunoResearch Inc. (West Grove, PA, USA). The Gαs activation assay kit (Cat. No. 80801) was purchased from New East Biosciences (King of Prussia, PA, USA). The 125I-cAMP radioimmunoassay (RIA) kit was obtained from the Nuclear Medicine Laboratory of Shanghai College (Shanghai, China). An enzyme-linked immunosorbent assay (ELISA) kit for PGE2 was purchased from Research & Development Co., Ltd. (MN, USA). Phospho-GRK2 (pSer670) was obtained from Pierce Biotechnology (Rockford, IL, USA). A Lipofectamine 2000 transfection kit from Invitrogen (Carlsbad, CA, USA) was used as suggested by the supplier. The SuperScript First-Strand Synthesis System for RT-PCR kit (Cat. No. 11904–018) was purchased from Invitrogen (Carlsbad, CA, USA). The primers for EP4, TRAF2 and GAPDH were obtained from Invitrogen (Shanghai, China). TRAF2 and control small interfering RNA (siRNA) were synthesized by Invitrogen (Shanghai, China). The control sequence does not match any human genome sequence.
Induction of CIA
CIA was induced by immunizing rats with native chicken CII that had been dissolved overnight at 4 °C in 0.1 M acetic acid (2 mg/mL) and emulsified with an equal volume of IFA. Rats were injected with 0.2 mL (collagen: 200 mg) of the emulsion subcutaneously at the base of the tail. A booster injection was given on day 7 after the initial immunization. Control rats were injected with vehicle. The day of the first immunization was defined as day 0.
In vivo drug administration
After the onset of CIA (around 14d), model rats were randomly divided into CIA-Veh, CIA-MTX and CIA-Paroxetine groups according to the clinical scores. MTX (0.5 mg/kg every 3 days) and paroxetine (15 mg·kg−1·d−1) were dissolved in 0.5% CMC-Na and administered by gavage for 15 days.
Arthritis assessment
Rats were inspected daily for signs of arthritis by two independent observers who were not aware of the treatments. The uninjected hind paw volume was measured with a YLS-7B toe volume meter. Paw swelling (▵mL) was calculated by subtracting the paw volume at day 0. The arthritis severity of each paw was evaluated by using a macroscopic scoring system ranging from 0 to 4 (0, paws with no swelling or focal redness; 1, mild but definite redness and swelling of the ankle or wrist or apparent redness and swelling limited to individual digits, regardless of the number of affected digits; 2, moderate redness and swelling of the ankle or wrist; 3, severe redness and swelling of the entire paw, including digits; and 4, maximally inflamed limbs with the involvement of multiple joints). The cumulative score for all four paws of each mouse was used as the polyarthritis index and had a maximum value of 16 [18].
Histopathological joint examination
The hind paws of rats were removed on day 28, fixed with neutral buffered 10% formalin, decalcified for 10 days with EDTA and embedded in paraffin for histological analysis. The paraffin sections were stained with hematoxylin and eosin. The micrographs were evaluated histologically by two independent observers.
The severity of arthritis in the joint was graded from 0 to 4 according to the intensity of lining layer hyperplasia, mononuclear cell infiltration, and pannus formation (grade 0, normal ankle joint; grade 1, normal synovium with occasional mononuclear cells; grade 2, definite arthritis with a few layers of flat to rounded synovial lining cells, scattered mononuclear cells and dense infiltration of mononuclear cells; grade 3, clear hyperplasia of the synovium with three or more layers of loosely arranged lining cells and dense infiltration of mononuclear cells; and grade 4, severe synovitis with pannus formation and erosions of articular cartilage and subchondral bone) [19].
FLS culture
Rat FLSs were obtained from the joint synovia of normal SD rats or CIA rats as specified. Normal hFLSs were isolated from primary synovial tissue obtained from trauma patients who had undergone total joint replacement surgery or synovectomy, and RA hFLSs were isolated from primary synovial tissue obtained from RA patients as indicated [20]. Informed consent was obtained from all patients, and the study protocol was approved by the Anhui Medical University Ethics Committee. FLSs were grown in DMEM with 10% fetal bovine serum (FBS) at 37 °C in a humidified atmosphere of 5% CO2. Passage 3–8 cells were used for subsequent experiments.
FRET analysis of cAMP
YFP/CFP PM-ICUE3 adenovirus was added to the culture medium of rat FLSs, which was changed to fresh complete medium 4 h later. Forty-eight hours after infection, cells coexpressing the cAMP sensor (ICUE) were plated in imaging dishes and imaged in the dark at 37 °C on a temperature-controlled stage using a Leica DM IRB (Leica Microsystems) microscope with a CoolSnap Fx cooled charge-coupled device camera (Ropper Scientific) controlled by MetaFluor 7.5 (Universal Imaging Corporation, Downingtown, PA, USA). Dual emission ratio imaging was carried out using a 436DF10 excitation filter, a 436–510 DBDR dichroic mirror, and 480-AF30 and 550-AF30 emission filters for CFP and YFP, respectively. The exposure time was 200 ms, and images were taken every 20 s. Typically, equal sensor-positive cells and nonspecific areas were chosen in the microscopic field. The development of fluorescence was recorded individually in each area for the duration of the experiment. Several independent plates were analyzed according to this procedure, and the specific FRET signals of each cell (positive minus negative area) were pooled. Cells displaying a range of intensities were selected and analyzed without any impact of the sensor expression level on the responsiveness noted. After 5 min of baseline measurement, cells were stimulated with 20 ng/mL TNF-α, the control group was not stimulated, and 1 μM PGE2, Butaprost or ONO-4819 was added as a positive control after 20 min of stimulation. A low hormone dose was chosen to avoid saturation of the ICUE sensor, which has a limited dynamic range compared to that of BRET assays. The fluorescence intensity of nonspecific areas was subtracted from the intensity of fluorescent cells expressing the sensor to quantify the specific signal. The FRET ratio (CFP/YFP) was calculated for each individual cell. The data represent the mean ± SEM of at least 20 individual cell responses measured in three independent experiments.
Flow cytometric analysis
FLSs were detached with 1 mM PBS EDTA, fixed with 4% paraformaldehyde, and then suspended in fluorescence-activated cell sorting (FACS) buffer (PBS/0.1% bovine serum albumin/0.01% NaN3). A total of 1 × 105 cells were incubated with specific primary antibodies overnight at 4 °C and then incubated for 2 h with the corresponding fluorescently labeled secondary antibodies. As a negative control, FLSs were incubated with fluorescently labeled secondary antibodies only. Cells were then washed, resuspended in FACS buffer and analyzed by flow cytometry (FC500, Beckman). Data analysis was performed using Cell Quest™ analysis software.
Determination of hFLS viability
To test the effect of TNF-α on hFLS proliferation, hFLSs were seeded in triplicate in a 96-well plate at 1.5 × 104 cells/well and cultured for 6 h. The cells were then treated with TNF-α (20 ng/mL) or TNF-α + INN (20 ng/mL + 1 × 10−6 M) for 48 h. The number of metabolically active mitochondria and viable cells was determined at 490 nm using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Measurement of cAMP concentrations
After the indicated treatments in 24-well plates, cell culture supernatants were discarded, and hFLSs (5 × 104 cells/well) were washed with ice-cold PBS. The cells were then dissociated in lysis buffer and centrifuged at 1500 × g for 3 min at 4 °C, and the supernatants were immediately frozen for later use. The concentration of intracellular cAMP was measured using a 125I-cAMP RIA kit according to the instructions specified by the manufacturer [21].
Protein extraction and membrane preparation
To prepare total cell lysates, FLSs (1 × 105 cells/well) in 6-well plates were washed with ice-cold PBS and lysed in ice-cold lysis buffer containing 50 mM Tris, 150 mM NaCl, 1% Nonidet P-40, and protease inhibitor cocktail (Sigma-Aldrich) for 40–50 min at 4 °C. The lysates were centrifuged for 4 min at 3000 rpm, and the resulting supernatants were diluted to 1 mg protein/mL and kept frozen at −80 °C to analyze total protein expression. Membrane fragments were obtained from the supernatant by centrifugation at 30,000 × g for 2.5 h, and the pellet was resuspended in PBS [22]. The efficiency of protein extraction was determined using pan-cadherin, a general plasma membrane marker, as described previously [23]. The protein content was determined by standard bovine serum albumin protein assays.
Coimmunoprecipitation
FLSs were lysed in 0.5 mL of lysis buffer and centrifuged for 10 min at 10,000 × g in a refrigerated centrifuge. The supernatant was collected, mixed with 5× Laemmli buffer, and then boiled for 5 min. The whole-cell lysate was precleared for 30 min at 4 °C with 20 μL of protein A+G agarose. The precleared cell lysates were subsequently incubated with 2 μg of the primary antibody for 1 h, 20 μL of protein A+G agarose beads was added, and the incubation was continued overnight at 4 °C with constant rotation. Next, the agarose beads were pelleted by centrifugation at 2500 rpm for 5 min at 4 °C. After extensive washes with ice-cold lysis buffer, the beads were resuspended in sample loading buffer, boiled for 5 min, and subjected to SDS-polyacrylamide gel electrophoresis, and the interaction of both proteins was detected by the corresponding primary antibody [24].
Western blotting
Proteins were separated by SDS-PAGE, electroblotted onto PVDF membranes (Bio-Rad), blocked for 2 h in 5% nonfat milk in TBS-0.1% Tween and incubated with the indicated primary antibodies diluted 1:1,000 in primary antibody dilution buffer (Beyotime, China) and the appropriate secondary antibody diluted 1:10,000 in 5% nonfat milk in TBS-0.1% Tween. Autoradiographs were detected using a LAS400Mini Chemilux CCD camera (GE Healthcare). All the experiments reported in this study were performed three times, and the results were reproducible.
Laser confocal microscopy
Immunofluorescence analysis was performed as described previously [25]. Briefly, hFLSs were seeded in chamber slides, fixed in 4% paraformaldehyde at room temperature, permeabilized with 0.2% Triton X-100, blocked with 1% BSA at 37 °C for 0.5 h in a humidified chamber, incubated with anti-TNFR1, anti-TNFR2, anti-TRAF2, or anti-GRK2 overnight at 4 °C, washed with PBS three times, and then incubated with Alexa Fluor 488-conjugated or Cy3-conjugated secondary antibodies (Invitrogen) at 37 °C for 2 h. Following three washes in PBS, DNA was counterstained with DAPI for 5 min. Fluorescent images were collected under a Leica SP5 laser scanning confocal microscope. The gray value per pixel was measured by the plot profile function of ImageJ software. The area under the curve (AUC) was used to compare the fluorescence intensity, including protein expression and distribution on the cell membrane and in the cytoplasm and total cells. The average relative fluorescence intensity (AFI) of protein expression in total cells was quantified using ImageJ software (version 1.42q, NIH).
High content screening
hFLSs were seeded in 96-well plates, fixed in 4% paraformaldehyde at room temperature, permeabilized with 0.2% Triton X-100, blocked with 1% BSA at 37 °C for 0.5 h in a humidified chamber, washed with PBS three times, and counterstained with DAPI for 5 min. Fluorescent images were taken under a mage Xpress Micro 4 high content screening system.
Small interfering RNA transfection
Before transfection, hFLSs were cultured to 70%–80% confluence at 37 °C as described previously and then transfected with the siRNA using Lipofectamine 2000 transfection reagent according to the manufacturer’s protocol. hFLSs were transiently transfected with 50 nmol of siRNA. The transfection efficiency was analyzed by RT-PCR or Western blotting after 24 h, and the maximum transfection efficiency of each siRNA was determined. The siRNA sequences were as follows: TRAF2 sense: 5’-AAUGCCGGCAGCUCAGGCUUCUCUC-3’; TRAF2 antisense: 5’-GAGAGAAGCCUGAGCUGCCGGCAU-3’; negative control sense: 5’-UUCUCCGAACGUGUCACGUTT-3’; and antisense: 5’-ACGUGACACGUUCGGAGAATT-3’.
Real-time qPCR
Total RNA was prepared using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). First strand cDNA was synthesized from total RNA (1 μg) by reverse transcription (Fermentas). PCR products were analyzed on a 1.5% agarose gel and stained with ethidium bromide for UV detection. Real-time PCR assays were performed with an Applied Biosystems 7500 fast real-time PCR system (ABI) using SYBR® green real-time PCR master mixe (Invitrogen). The oligonucleotide primer sequences were as follows: EP4 forward primer, 5’-AAGTCGCGCAAGGAGCAGAA-3’; EP4 reverse primer, 5’-CTTGTCCACGTAGTGGCTGT-3’ (270 bp); TRAF2 forward primer, 5’-AGATTGAAGCCCTGAGTAGCAAGG-3’; TRAF2 reverse primer, 5’-ATCACCACAAAGAAGAGGGACAGG-3’ (304 bp); GAPDH forward primer, 5’ -ACCACAGTCCATGCATCAC-3’; and GAPDH reverse primer, 5’-TCCACCACCCTGTTGCTGTA-3’. Quantitative analysis was performed using the ratio of the target gene to GAPDH. All results are representative of at least three experiments [26].
Molecular docking analysis
Potential binding sites of GRK2 and TRAF2 were calculated using Discovery Studio (DS, version 2020). The crystal structure of human GRK2 (PDB ID: IYM7) and the TRAF domain of human TRAF2 (PDB ID: 1CA4) were obtained from RCSB Protein Data Bank 1. DS protein-protein docking was used to identify the interaction between GRK2 and TRAF2 and the potential binding sites. The lower energy pose indicated that stronger binding occurred.
Statistical analysis
All data were analyzed by GraphPad Prism 7 statistical software and are expressed as the mean ± SEM. Differences between multiple groups were evaluated by either one-way ANOVA with Tukey’s posttest or two-way ANOVA with Sidak’s posttest. A t-test was used to compare differences between two groups. P values less than 0.05 were considered to be significant. Western blotting and RT-PCR were repeated at least three times for each sample, and the results were subjected to semiquantitative analysis to ensure maximal accuracy of the conclusions drawn from these data.
Results
EP4 signaling is blocked in TNF-α-stimulated FLSs
We previously showed that after TNF-α stimulation, the cAMP concentration in FLSs in SD rats was decreased [17], and cAMP is the key molecule in GPCR signaling. Many proinflammatory cytokines, such as TNF-α, IL-1, PGE2, IL-17, IL-6, and GM-CSF, and chemokines are abundant in RA patients regardless of therapy [27]. PGE2 is produced in response to proinflammatory cytokines and plays an important pathogenic role through its receptors [28]. Among these important factors, PGE2 receptors (EPs) are GPCRs.
Accordingly, we investigated the mechanism by which TNF-α decreased cAMP expression in FLSs. FLSs, which were isolated from the synovia of rats, were cultured in vitro in the presence of TNF-α (at final concentrations of 0, 0.02, 0.2, 2, or 20 ng/mL) for 48 h, and then cAMP levels were analyzed. At concentrations of 2 or 20 ng/mL, TNF-α notably decreased the cAMP level in FLSs (Fig. 1a). Rat FLSs were infected with adenovirus encoding PM-ICUE3, which is a cAMP fluorescence resonance energy transfer (FRET) biosensor anchored on the plasma membrane. PGE2 (1 μM) induced abundant cAMP production in FLSs; however, PGE2 signaling was attenuated by TNF-α (20 ng/mL) pretreatment for 48 h. Since the PGE2 receptors EP2 and EP4 have been reported to be expressed on FLSs, we used specific EP2 and EP4 agonists to determine which receptor subtype was affected by TNF-α. Both the EP2 agonist butaprost and the EP4 agonist ONO-4819 triggered cAMP production in rat FLSs; notably, ONO-4819-induced cAMP signaling was significantly reduced by TNF-α pretreatment (Fig. 1b), indicating that EP4 function was blocked by chronic TNF-α stimulation. We then analyzed changes in the membrane expression of EP2 and EP4 in response to TNF-α stimulation. In accordance with the FRET data, 48 h of treatment with increasing concentrations of TNF-α decreased the membrane distribution of the EP4 subtype but not EP2 (Fig. 1c, d). A scatter plot of the membrane expression of EP4 and the concentration of TNF-α revealed a negative correlation (Fig. 1e) (R2 = –0.7805, P = 0.0469). This correlation suggested that the membrane expression of EP4 was reduced upon TNF-α stimulation. Further analysis showed a significantly positive correlation between intracellular cAMP levels and EP4 membrane expression (R2 = 0.989, P = 0.0005) (Fig. 1f). Since FLSs are able to produce PGE2 under TNF-α stimulation, to eliminate the effect of endogenous PGE2 on the receptors, indometacin (INN) was added to the system to inhibit PGE2 synthesis. Induction with either TNF-α or TNF-α plus INN greatly decreased the membrane expression of EP4 but had no obvious effect on EP2 distribution. Consistent with the data reported, no expression of EP1 or EP3 was detected on the surface of rat FLSs by flow cytometry (Fig. 1g–i). These data indicate that TNF-α-induced downregulation of cAMP production is related to the internalization of EP4 in rat synoviocytes. In the following experiments, we confirmed this phenomenon in primary hFLSs and further investigated the potential mechanism of TNF-α-induced EP4 internalization in hFLSs. Analysis of the antibody-specific band showed that both total and cytoplasmic EP4 levels were elevated by TNF-α or TNF-α plus INN treatment (Fig. 1j, k). In contrast, membrane EP4 distribution was substantially reduced (Fig. 1l). As revealed by the present data, total and cytoplasmic EP4 expression was lower in the TNF-α plus INN group than in the TNF-α alone group, suggesting a role of PGE2 in the response to TNF-α. It is known that EP4 couples with Gαs. Further research indicated that total Gαs expression was not notably changed under TNF-α or TNF-α plus INN stimulation (Fig. 1m). However, both treatments obviously decreased GTP-bound Gαs, which was activated. Interestingly, the activation of Gαs in hFLSs stimulated with TNF-α plus INN was higher than that in TNF-α-stimulated cells, suggesting that PGE2 may attenuate the activation of Gαs (Fig. 1n). These data suggest that TNF-α directly induces EP4 receptor desensitization and internalization in FLSs.
TNF-α impairs EP4 signaling in hFLSs in a TNFR2-dependent manner
TNF-α is known to induce significant hFLS proliferation. The TNFR superfamily induces diverse biological effects, including cell survival, proliferation, differentiation, and apoptosis [29, 30]. In the present study, the membrane expression of TNFR1 and TNFR2 in hFLSs was examined by flow cytometry and Western blotting. The results showed that membrane TNFR2 was obviously increased in response to TNF-α stimulation, but there was no significant change in TNFR1 expression (Fig. 2a–d). We silenced TNFR1 and TNFR2 gene expression by small interfering RNA (siRNA) transfection. The protein expression levels of TNFR1 and TNFR2 were measured by Western blotting to determine the optimal sequence. TNFR1 and TNFR2 protein expression in hFLSs was reduced by ~82.7% and 84.2%, respectively, in response to TNFR1- and TNFR2-specific siRNA compared with the negative control sequence (Fig. 2e, f). To determine which TNF-α receptor subtype is involved in this process, we used siRNA to knock down TNFR1 or TNFR2 in hFLSs. High content screening was used to analyze hFLS proliferation in response to TNF-α stimulation. Under TNF-α stimulation, TNFR1-silenced hFLSs showed no changes in proliferation, but the proliferation of TNFR2-silenced hFLSs was reduced compared with that of normal hFLSs (Fig. 2g, h). Silencing TNFR2 but not TNFR1 decreased EP4 expression in response to TNF-α stimulation, as observed by laser confocal microscopy (Fig. 2i, j). The gray value per pixel was determined using the plot profile method. The AUC was used to compare the fluorescence intensity. These data suggest that TNF-α impairs EP4 signaling in hFLSs via TNFR2 but not TNFR1.
TNF-α impairs EP4 signaling in hFLSs in a TNFR2- and TRAF2-dependent manner
We designed the following experiments to confirm the role of TRAF2 in regulating EP4-cAMP signaling. The total protein expression of TRAF2 was significantly elevated by TNF-α stimulation compared with that in normal hFLSs, but TRAF2 expression was higher in hFLSs after stimulation with TNF-α plus INN than in those stimulated with TNF-α alone (Fig. 3a). In other words, TNF-α-induced PGE2 diminished the expression of TRAF2. Evidence has shown that TRAF2 signaling is regulated by translocation. Recruitment of TRAF2 to plasma membrane lipid rafts is important for NF-κB signaling activation [31]. We then examined the expression of TRAF2 on the cell membrane. Consistent with the previous result, membrane TRAF2 expression was substantially increased upon stimulation by TNF-α plus INN or TNF-α alone, as detected by both Western blotting and laser confocal microscopy (Fig. 3b, c).
To determine the exact role of TRAF2, we silenced TRAF2 gene expression by siRNA transfection. The protein and gene expression levels of TRAF2 were measured by Western blotting and real-time qPCR, respectively, to determine the optimal sequence. TRAF2 gene expression was reduced by ~83.9%, while protein expression in hFLSs was reduced by ~89.9% in response to TRAF2-specific siRNA compared with the response to the negative control sequence (Fig. 3d). Subsequently, we found that TNF-α alone or in combination with INN obviously promoted hFLS viability. However, TNF-α alone increased cell viability compared with TNF-α plus INN administration. After TRAF2 knockdown, the effect of TNF-α plus INN on hFLS viability was significantly blocked (Fig. 3e). However, endogenous PGE2 produced by hFLSs under TNF-α induction slightly promoted hFLS proliferation, suggesting that this effect may occur through a TRAF2-independent pathway. Thus, TRAF2 is clearly involved in TNF-α-induced hFLS proliferation.
The concentrations of cAMP were measured, and the results showed that in response to TNF-α alone or in combination with INN, intracellular cAMP levels were significantly decreased. However, in TRAF2-defective hFLSs, TNF-α alone or in combination with INN failed to change the levels of cAMP (Fig. 3f), indicating that TRAF2 played a clear role in the TNF-α-induced decrease in cAMP production in hFLSs. Total and membrane EP4 protein levels were prominently increased; specifically, the level of membrane EP4 was much higher in response to TNF-α plus INN stimulation than in response to TNF-α stimulation alone. In TRAF2-silenced hFLSs, cytoplasmic EP4 expression was remarkably elevated in TNF-α-stimulated cells but was significantly decreased in cells induced by TNF-α plus INN (Fig. 3g–j), indicating that PGE2 may slightly reduce EP4 membrane translocation. EP4 expression levels were determined by Western blotting and real-time qPCR and were prominently decreased by TRAF2 siRNA (Fig. 3k).
TNF-α triggered translocation of the TRAF2-GRK2 complex to the cell membrane and induced complex dissociation
TNFR2 is located on the cell membrane, and TRAFs have high TNFR2 binding capacities and are widely accepted to be common signal transducers for TNFR2. To explore how TNF-α promotes the desensitization of the EP4 receptor, we further tested whether TRAF2 interacts with GRK2/β-Arrestin2 using a coimmunoprecipitation assay. After stimulation with TNF-α or TNF-α plus INN, the interaction between TRAF2 and GRK2 was significantly diminished, as shown in Fig. 4a. Then, the distribution of the TRAF2-GRK2 complex was systematically examined. In primary hFLSs, the complex was principally located in the cytoplasm and hardly expressed on the cell membrane. TNF-α along or in combination with INN for 48 h significantly inhibited the association of TRAF2 and GRK2 in hFLSs (Fig. 4b, c), but the interaction between TRAF2 and β-arrestin2 did not change significantly (Fig. 4d).
Next, we found that GRK2 distinctly translocated to the cell membrane in response to both treatments (Fig. 4e), and there was a significant decrease in cytoplasmic GRK2 (Fig. 4f). Laser confocal microscopy revealed synchronous membrane translocation of both TRAF2 and GRK2 (Fig. 4g). To track the translocation of the TRAF2-GRK2 complex and capture the time point at which they dissociated under TNF-α stimulation, we examined the protein complex at different time points (0, 5, 10, 15, 30, and 60 min) in the cytoplasm and cell membrane. Figure 4h shows that the association of TRAF2 and GRK2 in the cytoplasm was reduced gradually after stimulation with TNF-α. The complex transferred to the cell membrane within 15 min, and then the two proteins separated from each other gradually, as detected at 30 and 60 min.
Next, we observed the interaction between these two proteins in vivo. TNF-α (20 ng/mL) was injected into the knee joint of normal SD rats. After 0, 5, 10, 15, 30, and 60 min, the synovium was collected, and then cytoplasm and membrane proteins were isolated. We noted that coimmunoprecipitation was increased in the cell membrane after treatment with TNF-α for 10 to 15 min and then decreased. Less cytoplasmic TRAF2-GRK2 interactions were found in TNF-α-challenged rats than in saline-stimulated rats in a time-dependent manner, which was consistent with the in vitro results (Fig. 4i). To further identify the interaction between GRK2 and TRAF2, we used semiflexible small molecule and protein docking analysis on a DS platform. The target sequence of GRK2 was obtained from 3V5W alignment of the RCSB PDB database. GRK2 is composed of three different domains: an RGS homology (RH) domain, a protein kinase domain and a pleckstrin homology (PH) domain [32]. One feature of TRAF family members (except TRAF7) is the protein-protein interaction domain with ~230 amino acid residues at the C-terminus, which is known as the TRAF domain. The TRAF domain is subdivided into two different subdomains: the TRAF-N domain (a coiled-coil domain) and the TRAF-C domain (composed of 7 to 8 antiparallel β-chain folds). Members of the TRAF family form a mushroom-like trimeric structure in solution with TRAF domains, which is the functional unit of TRAF [33]. PDB ID: 1CA4 is the structure of the TRAF domain of human TRAF2. The best binding form with the lowest binding energy is presented in Fig. 5a, b.
TRAF2 promotes EP4 desensitization by activating GRK2 which contributes to hFLS proliferation
How is GRK2 recruited to the cell membrane in response to TNF-α stimulation? Analysis of the membrane and cytoplasmic protein levels of GRK2 in TRAF2-deficient hFLSs showed that membrane GRK2 was prominently decreased compared with that in the control siRNA group (Fig. 6a); conversely, cytoplasmic GRK2 was increased in both stimulation groups compared with that of hFLSs transfected with control siRNA (Fig. 6b). These results suggest that TNF-α modulates EP4-Gαs-cAMP signaling through the heterogeneous TRAF2-GRK2 complex. Thus, the interaction between TRAF2 and GRK2 may play a pivotal role in the crosstalk between the TNF-α and EP4 signaling pathways. In addition, we concluded that TRAF2 binds with GRK2 and is mainly distributed in the cytoplasm of normal primary hFLSs. The complex dissociates upon TNF-α stimulation, and both TRAF2 and GRK2 simultaneously translocate to the cell membrane, resulting in desensitization and internalization of EP4. GRK2 fails to be recruited to the cell membrane in the absence of TRAF2, indicating that TRAF2 promotes GRK2 translocation in the presence of TNF-α. In addition, as shown in Fig. 6c, measurement of phospho-GRK2 in the hFLS membrane revealed that TRAF2 also contributes to the activation of GRK2. Paroxetine, a selective serotonin reuptake inhibitor, was found to inhibit GRK2 [34]. To verify the effect of GRK2 on TNF-α-induced hFLS proliferation, paroxetine was added. The results suggest that inhibiting GRK2 attenuates TNF-α-induced hFLS proliferation (Fig. 6d).
Paroxetine treatment attenuated the symptoms of CIA rats
In FLSs from patients with RA, the concentration of cAMP was lower than that in primary hFLSs, while cell viability was enhanced (Fig. 7a, b). Accordingly, we investigated the relationship between the TNF-α-induced decrease in cAMP and the membrane expression of EP 1, 2, 3, and 4 in CIA FLSs by Western blotting. As shown in Fig. 7c, the uninjected hind paw appeared significantly swollen and enlarged on day 28. The cAMP concentration of CIA FLSs was lower than that of normal rat FLSs, and cell viability was enhanced (Fig. 7d, e). Body weight and pathological manifestations were observed every two days after the booster injection. CIA rats showed severe body weight loss, with significantly higher clinical manifestation scores, arthritis indexes, numbers of swollen joints and sizes of secondary paw swelling than control rats (Fig. 7f–j). MTX is a widely used RA treatment, and both paroxetine and MTX clearly exerted positive effects on alleviating the symptoms of CIA rats. Although there was no significant difference compared with that in the MTX group, paroxetine treatment restored the body weights of CIA rats. Regarding the alleviation of global symptoms in CIA rats, paroxetine exerted moderate therapeutic effects compared with those of MTX. Pathological changes in CIA joints were analyzed by H&E staining. Normal joints had only 1–3 layers of synoviocytes, and histological examination of CIA joints showed excessive synoviocyte proliferation and disorder, accompanied by a large amount of lymphocyte infiltration, abundant pannus formation, obvious cartilage erosion and evident local inflammation (Fig. 7k). As expected, MTX significantly reduced synovial inflammation and bone destruction in CIA joints. Paroxetine significantly prevented histological damage to the joints of CIA rats.
Discussion
Proinflammatory cytokines such as TNF-α and PGE2 participate in the regulation of inflammatory joint diseases, including RA. The high levels of TNF-α in the RA synovium play an important role in synovial hyperplasia in RA by suppressing apoptosis and promoting the proliferation of synoviocytes through NF-κB-dependent signaling pathways mediated by upregulated TNFR2 and TRAF2 [35]. TNF-α also stimulates synovial fibroblasts to secrete mediators such as PGE2, matrix metalloproteinases (MMPs), and vascular endothelial growth factor (VEGF), which regulate inflammation, connective tissue degradation, and angiogenesis, respectively [36]. Among these important proinflammatory factors in the RA synovial microenvironment, only PGE2 receptors (EPs) are GPCRs [37]. Our previous experiments showed that TNF-α also plays a critical role in cAMP production. This information led us to hypothesize that TNF-α regulates GPCR signaling in hFLSs through downstream molecules. Therefore, in our present study, we screened the potential target GPCR of TNF-α in CIA FLSs. EP2 and EP4 were abundantly expressed in both CIA FLSs and normal hFLSs, which was consistent with other reported findings [38]. According to the correlation coefficient analysis, TNF-α stimulation induced EP4 internalization from the cell membrane, and this change directly decreased the cAMP concentration, which indicates that the reduction in cAMP in TNF-α-stimulated hFLSs is primarily due to EP4 desensitization and internalization. EP2 and EP4 often mediate similar effects, but responses to EP4 stimulation are typically more prominent [39].
We used normal hFLSs to examine the complex networks of proinflammatory cytokines in RA synovial fibroblasts, and hFLSs were stimulated with TNF-α with or without INN to examine the mechanism by which EP4 is regulated by TNF-α. Indometacin is a nonselective inhibitor of cyclooxygenase (COX) 1 and 2 and inhibits the endogenous production of prostaglandins [40]. Our results indicated that TNF-α obviously promoted the proliferation of hFLSs in a manner dependent on the combined actions of TNF-α and TNF-α-induced PGE2. The administration of INN eliminated the specific effects of PGE2 on EPs, providing a better observation of the function of TNF-α. TNF-α induces hFLS hyperplasia by binding with TNFR2, which activates the transcription factor nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) [41]. The mechanisms mediating these activations depend on the recruitment of TRAFs to the intracellular region of the receptor [42, 43]. Recruitment of TRAF2 to plasma membrane lipid rafts is important for NF-κB signaling activation [31]. TRAF2 is currently the best-characterized TRAF family member [44] and plays a key role in the signal transduction of TNFR1 and TNFR2 [41]. Genetic evidence indicates that TRAF2 is necessary for the ubiquitination of receptor interacting protein 1 (RIP1), which then serves as a platform for the recruitment and stimulation of IκB kinase (IKK), leading to the activation of NF-κB [45]. NF-κB is highly activated in RA synovial fibroblasts (RASFs) [46] and lining cells, produces a strong survival signal and links inflammation and a decrease in apoptosis [47] (Fig. 8). The membrane expression levels of TNFR2 and TRAF2 in hFLSs were obviously increased after TNF-α stimulation. Moreover, the expression of TRAF2 was significantly increased after the depletion of endogenous PGE2 by INN. This finding suggests that TNF-α–induced PGE2 production may diminish TRAF2 expression, indicating that PGE2 may play a considerable role in the TNF-α signaling pathway.
The data obtained in our study demonstrated that TRAF2 could negatively regulate EP4-Gαs-cAMP signaling by promoting the recruitment of GRK2 to the plasma membrane and GRK2 phosphorylation. TRAF2 associates with GRK2 in the cytoplasm of normal hFLSs. In response to TNF-α, GRK2 is passively pulled to the cell membrane by the translocation of TRAF2 within 15 min, and GRK2 is then released and phosphorylated on the membrane, inducing excessive desensitization and internalization of EP4. The functional loss of EP4 attenuates the activation of Gαs and ultimately leads to a reduction in cAMP. cAMP inhibits cell growth by blocking growth factor activation of extracellular signal-related kinases (ERKs) [48]. The TNF-α-mediated reduction in cAMP production contributes to further proliferation and activation of hFLSs in a manner independent of the TNFR pathway, which indicates a new pattern of TNF-α actions in RA.
TNF blockers, such as etanercept, infliximab, adalimumab, and golimumab, suppress the immune system by interfering with the effects of TNF-α and have been shown to be highly effective in treating RA. However, increased frequencies of infection, cancer and neurological side effects have been reported during TNF therapy [49]. TNF-α can not only cause inflammation but also plays an important role in host defense, particularly in the killing of intracellular microorganisms such as Listeria and Mycobacteria and in inducing apoptosis in some tumor cells [50]. Consequently, there has been some concern that long-term inhibition of TNF is not a medically satisfying approach for RA treatment [51]. Specifically, attenuating TNF-α activation and blocking crosstalk with other stimulatory signals may be a potent therapeutic strategy.
GRK2 is a family of protein kinases that regulates the activity of GPCRs by phosphorylating their intracellular domains after the associated G proteins have been released and activated. Many studies have been elucidated the function of GRK2 in regulating EPs [52,53,54]. Shi and colleagues reported that similar to GRK2, the kinases apoptosis signal-regulating kinase 1 (ASK1) and germinal center kinase (GCK) can bind with the TRAF domain of TRAF2, and the RING finger domain is needed for their activation [55]. The TRAF2-binding region of ASK1 is the amino- and carboxyl-terminal noncatalytic domain [56]. Other researchers have shown that NF-κB-inducing kinase (NIK) association with TRAF2 requires the conserved WKI motif within the TRAF domain and mediates TNF activation of NF-κB [57]. This evidence could provide clues to map the binding domain regions of TRAF2 and GRK2.
TNFR1 contains a death domain in its intracellular region, but TNFR2 does not [58]. Activation of TNFR1 induces the formation of two different TNF receptor signaling complexes. Complex I controls the expression of antiapoptotic proteins that prevent activation of cell death processes, whereas Complex II triggers cell death processes [59]. We noted that silencing TRAF2 using siRNA caused significant increases in cAMP levels. This result is consistent with the changes in hFLS proliferation induced by TRAF2 siRNA. After TRAF2 knockdown, the TNF-α plus INN group almost completely lost the ability to promote hFLS proliferation. TRAF2 deficiency did not significantly affect the TNF-α-induced generation of PGE2 in hFLSs.
GPCR functions are precisely controlled by G protein-coupled receptor kinases (GRKs) and β-arrestins. GPCR desensitization is triggered by the GRK-mediated phosphorylation of ligand-activated GPCRs within the third intracellular loop or carboxy-terminal tail [60]. GRKs include seven known subtypes (GRK1-GRK 7), of which four members, GRK2, -3, -5, and -6, are ubiquitously expressed [61]. In particular, GRK2 and GRK6 are involved in the abnormal proliferation of FLSs in RA models [62, 63]. In our study, to determine the mechanism by which TNF-α promotes EP4 desensitization, we assessed whether TRAF2 interacts with GRK2/β-arrestin2 in hFLSs. The results showed that TRAF2 could interact with both GRK2 and β-arrestin2 in hFLSs. Interestingly, we further found that TRAF2 interacts with GRK2 to prevent it from participating in the regulation of EP4 receptor desensitization under normal conditions. However, in response to TNF-α with or without INN, the ability of TRAF2 to interact with GRK2 was weakened, which indicated that the dissociation of GRK2 was increased. Then, EP4 desensitization was increased, and the relatively high intracellular levels of EP4 in hFLSs inhibited cAMP production and promoted cell survival. This is the first study to show the interaction between TRAF2 and GRK2, and TRAF2 can regulate the desensitization of EP4 through GRK2 under TNF-α stimulation.
To better understand this apparent shift in EP4 function, we carried out the next experiment. The TRAF2-GRK2 interaction was weakened after transfection with TRAF2 siRNA. Logically, the dissociation of GRK2 was greatly increased. However, the membrane level of EP4 was elevated. To further confirm these results, we found that membrane GRK2 was prominently decreased and cytoplasmic GRK2 was significantly increased after transfection with TRAF2 siRNA. These results suggested that TRAF2 could promote GRK2 translocation, but the mechanism by which TRAF2 controls the translocation of GRK2 to the plasma membrane remains unknown. Multiple mechanisms have been shown to regulate the association of GRK2 with the plasma membrane and GRK2 activity, including the regulation of GRK2 by other kinases, such as PKA, PKC, and MAPKs, during the activation of GPCRs [64]. An alternative explanation is that the increased activity and membrane availability of GRK2 in TNF-α-stimulated cells during GPCR activation may result from decreased binding of other cytosolic inhibitor proteins to GRK2 or increased binding of GRK2 to other cytosolic proteins to promote GRK2 activity. Another possible explanation is that the binding region of these two molecules is important for GRK2 activity and translocation, and these hypotheses need to be further investigated. In the future, we will determine (a) the structural domain responsible for the interaction between TRAF2 and GRK2 and (b) whether TRAF2 directly or indirectly modulates the translocation of GRK2.
According to the study results, TNF-α triggers the translocation of the TRAF2-GRK2 complex to the cell membrane, and GRK2 on the cell membrane may desensitize EP4, resulting in a reduction in cAMP levels. This TNF-α-TRAF2-GRK2-EP4 signaling pathway is involved in synoviocyte hyperplasia. TRAF2 and GRK2 are key factors in this mechanism and can be used as therapeutic targets for treating RA. However, no TRAF2 inhibitor is currently available, and so we used a GRK2 inhibitor to block this pathway and investigated its effect in treating arthritic animals. Paroxetine is a classic drug for treating depression and has been confirmed to have strong GRK2 inhibitory effects. Therefore, we used paroxetine to inhibit GRK2 to study whether GRK2 inhibitors could abrogate the reduction in cAMP in TNF-α-stimulated hFLSs, thereby effectively preventing the proliferation of inflammatory synovial cells. This experiment further verified the pathological role of the TNF-α-TRAF2-GRK2-EP4 signaling pathway in the abnormal proliferation of hFLSs. Due to the potential side effects in the central nervous system, paroxetine is not suitable for treating RA in the clinic. Because of the important role of GRK2 in many diseases, several GRK2 inhibitors with relatively mild side effects are undergoing preclinical research. MTX, a dihydrofolate reductase inhibitor, is a commonly used drug for treating RA. Our data showed that MTX effectively improved the clinical parameters of CIA rats and was even slightly better than paroxetine, but no significant differences between the two drugs were observed. According to the American Rheumatology Association, the effective rate of MTX in treating RA and animal models is less than 50%, and some patients give up treatment because of the severe side effects [65]. Therefore, the development of new therapeutic targets and new drugs for RA therapy is urgent. GRK2 is a promising target for the development of new antirheumatic therapies.
In this study on hFLSs, we determined that TNF-α affected the desensitization of EP4 and examined the effect of TNF-α on the expression and subcellular distribution of GRK2. These findings suggested that TRAF2 not only terminated TNF-α signaling but also regulated the GPCR signaling pathway by binding to GRK2 to control the dissociation of GRK2 from the membrane and then promoted the TNF-α-induced proliferation of hFLSs via the EP4-AC-cAMP pathway.
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (81973332, 81973314, 81673444, 81330081 and 81202541), the Anhui Provincial Natural Science Foundation for Distinguished Young Scholars (1808085J28), the Key Projects of Natural Science Research of Anhui Colleges and Universities (KJ2017A176), the Anhui University Excellent Youth Talent Support Program (gxyqZD2017025), the Innovation and Entrepreneurship Support Program for Returnees of Anhui Province (2017), The University Synergy Innovation Program of Anhui Province (GXXT-2020-066), the Program for Upgrading Scientific Research Level of Anhui Medical University (2019xkjT008), and the Program for Upgrading Basic and Clinical Collaborative Research of Anhui Medical University (2020xkjT033).
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YT, BH, PPG, ZW, ZWZ, MMW, HFS, YH, SLX and LLZ conducted the study and analyzed the data, YT, QTW and WW analyzed the data and wrote the paper.
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Tai, Y., Huang, B., Guo, Pp. et al. TNF-α impairs EP4 signaling through the association of TRAF2-GRK2 in primary fibroblast-like synoviocytes. Acta Pharmacol Sin 43, 401–416 (2022). https://doi.org/10.1038/s41401-021-00654-z
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DOI: https://doi.org/10.1038/s41401-021-00654-z
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