The rapid spread of antibiotic resistance among bacterial pathogens is a serious human health threat. While a range of environments have been identified as reservoirs of antibiotic resistance genes (ARGs), we lack understanding of the origins of these ARGs and their spread from environment to clinic. This is partly due to our inability to identify the natural bacterial hosts of ARGs and the mobile genetic elements that mediate this spread, such as plasmids and integrons. Here we demonstrate that the in vivo proximity-ligation method Hi-C can reconstruct a known plasmid-host association from a wastewater community, and identify the in situ host range of ARGs, plasmids, and integrons by physically linking them to their host chromosomes. Hi-C detected both previously known and novel associations between ARGs, mobile genetic elements and host genomes, thus validating this method. We showed that IncQ plasmids and class 1 integrons had the broadest host range in this wastewater, and identified bacteria belonging to Moraxellaceae, Bacteroides, and Prevotella, and especially Aeromonadaceae as the most likely reservoirs of ARGs in this community. A better identification of the natural carriers of ARGs will aid the development of strategies to limit resistance spread to pathogens.
Multi-drug resistant pathogens are increasing in prevalence worldwide [1,2,3]. The alarming rate at which bacteria adapt to antibiotics is partly due to their ability to acquire antibiotic resistance genes (ARGs) through horizontal transfer of mobile genetic elements (MGEs) such as plasmids. For example, plasmid-mediated resistance has emerged against quinolones , carbapenems , and colistin . In addition, other genetic elements such as integrons facilitate the acquisition and expression of ARGs by bacteria .
Numerous studies have revealed the diversity, abundance, and distribution of ARGs in habitats such as soil, rivers, human and animal guts, and wastewater treatment plants (WWTPs), implicating them all as plausible reservoirs for ARGs . Cultivation-independent metagenomics of environmental samples is a popular approach, but it often cannot assign a specific bacterial host to the ARGs and the MGEs they are encoded on . Specifically, it cannot identify the hosts of plasmids because total DNA extraction disconnects plasmids and chromosomes. This host-plasmid association is nevertheless critical to understand the ecology of antibiotic resistance and the trajectories that bring resistance genes into the clinic .
Proximity-ligation methods such as Hi-C and 3C have been used to detect interactions between DNA molecules originating in the same cell within microbial communities (Fig. 1). Both methods are able to reconstruct strain- and species-level genomes from mixed bacterial cultures, and correctly link plasmids and phage to their bacterial hosts [11,12,13]. These methods can also reconstruct metagenome-assembled genomes (MAGs) from bacterial communities such as those of the mammalian gut communities [14,15,16]. In this study, we showed that cultivation-independent metagenomic Hi-C data can help determine the reservoirs of ARGs and the plasmids and integrons that carry them in a diverse wastewater community. For example, we were able to determine in our wastewater sample that bacteria belonging to the Moraxellaceae, Bacteroides, Prevotella, and Aeromonadaceae are the most likely reservoirs of ARGs. It also led to the reconstruction of several novel MAGs.
Materials and methods
The wastewater sample was collected in October 2017 at the Moscow WWTP in Idaho (USA). The facility services ~25,000 people and collects mainly domestic wastewater. The sample was collected at the entrance of the WWTP, and 2 ml subsamples were centrifuged at 12,000 × g for 3 min. Pellets were archived at −20 °C until further processing.
Wastewater pellets were thawed on ice. In half of the aliquots we added a known number of E. coli K12::gfp containing plasmid pB10::rfp cells , hereafter named EC, such that they represented ~10% of the total bacterial community. Wastewater aliquots spiked with EC are named WWEC and aliquots consisting of the wastewater only are named WW.
The EC strain was grown overnight at 37 °C from a glycerol stock (−70 °C) in LB broth containing nalidixic acid (50 mg.L−1), kanamycin (50 mg.L−1), and tetracycline (10 mg.L−1). The culture was centrifuged for 3 min at 5000 × g and pellets were resuspended with the same volume of PBS. We estimated the number of bacteria in the wastewater and in the E. coli culture using three methods as described below.
First a qPCR approach was used to target the 16S rRNA gene . Reactions were carried out in a final volume of 10 µL, with 1 µL of pure and 10-fold diluted total genomic DNA from the WW or the bacterial culture, and the PerfeCta® qPCR ToughMix 2× (Quanta BioSciences™, Beverly, MA, USA). The assays were performed in duplicate with an Applied Biosystems StepOnePlus Real-Time PCR System (Applied Biosystems™, Waltham, MA, USA), and a standard plasmid containing the targeted sequence was used to construct a full standard curve in duplicate. The total bacterial cell count in the E. coli culture was estimated by dividing the number of 16S rRNA gene copies by the gene copy number in E. coli K12 (7). The total bacterial cell count in the water sample was estimated by dividing the number of 16S rRNA gene copies by 4.9, the average 16S rRNA gene copy number among 10,996 bacteria in the rrnDB database (https://rrndb.umms.med.umich.edu/). The WW sample contained an estimated number of 3.5 × 108 bacteria ml−1 and the E. coli culture 2.7 × 109 ml−1. Using the other two methods, flow cytometry and viable plate counts on R2A agar, the estimated bacterial counts were very similar: 3.3 × 108 and 2.7 × 107 per ml, respectively, for WW (only ~10% of bacteria in wastewater can be cultured), and 3.5 × 109 for the E. coli culture with both methods.
For the E. coli culture, total genomic DNA was extracted from 2 ml of the overnight culture using the GenEluteTM Bacterial Genomic DNA kit (Sigma-Aldrich, St. Louis, MO, USA).
Shotgun metagenomic libraries were prepared as follows. Total genomic DNA was extracted and isolated from WW and WWEC pellets using the DNeasy® PowerWater® kit (Qiagen, Venlo, Netherlands). PCR-free Illumina libraries for short insert length sequencing with Hiseq were made by the IBEST Genomics Resources Core (Moscow, ID, USA) using TruSeq® DNA PCR-Free library Prep kit (Illumina, San Diego, CA, USA). The Hi-C libraries were prepared from the WW and WWEC pellets using the ProxiMeta™ Hi-C preparation kit (Phase Genomics, Seattle, WA, USA).
We pooled the Hi-C and shotgun metagenomic libraries, and sequenced both samples using two lanes of HiSeq 4000, 2 × 150 bp paired-end reads at the University of Oregon sequencing core (Eugene, OR, USA). This produced 269,312,499 and 95,284,717 read pairs for the WW shotgun metagenomic and Hi-C libraries, respectively (ratio Hi-C:shotgun = 0.35), and 291,051,995 and 117,388,834 read pairs for the WWEC shotgun metagenomic and Hi-C libraries (ratio HiC:shotgun = 0.40).
Hi-C data analysis was performed using the ProxiMeta workflow as previously described . Some steps are described in more detail below.
Processing of the shotgun sequencing data
Shotgun sequencing data were processed using the following steps. Sequencing adapters were removed using BBDuk (BBTools developed by the Joint Genome Institute) with options k = 23, ktrim = r, mink = 12, hdist = 1, minlength = 50, –tpe, –tbo. Low-quality bases will be trimmed with BBDuk and options qtrim = rl, trimq = 10, minlength = 50, chastityfilter = True.
Processing of the Hi-C reads
Each set of reads was mapped to the metagenomic assemblies. Mapping was done using the Burrows-Wheeler alignment tool BWA-MEM [21, 22]. All reads that were incorrectly paired, unmapped, not uniquely mapped, mapped with a MAPQ score <20, or read pairs mapping to the same contig (which are not informative for deconvolution) were removed from the analysis.
Deconvolution of the Hi-C data
Deconvolution of contigs in the de novo assembly was performed using the ProxiMeta platform  that is partly based on the previously described method . Briefly, contigs <1000 bp in size, or which contained fewer than two restriction sites for the relevant enzyme were discarded for purposes of clustering. This dataset was normalized by the number of restriction sites on the contigs and contig Hi-C read coverage (which implicitly accounts for length and abundance, among other characteristics). Finally, the contigs were grouped into clusters based on their Hi-C linkages using a proprietary Markov Chain Monte Carlo algorithm.
Annotation of genome clusters
Genome clusters were compared with RefSeq genomes using Mash  to identify any close database matches for new genome clusters. Genome clusters were further analyzed using the CheckM  lineage_wf workflow with the –reduced_tree option to assess genome quality and estimate high-level phylogenetic placements for each cluster based on single-copy marker gene analysis. Some genome clusters were excluded on the basis of promiscuous interactions with other clusters, quantified as their vertex entropy in the Hi-C graph connecting genome clusters, calculated using the R entropy package . We excluded clusters with vertex entropy higher than 3. We assessed abundance of different organisms as the median of the abundance of constituent contigs >20 kb in size, estimated as kallisto “transcripts per million” . Circos plots were generated using Circoletto , and genome alignments to references were analyzed using quast v5.0.0 .
Marker gene detection in the metagenome assemblies
Detection of marker genes was done using BLAT with the options -minIdentity = 90, and hits for which the coverage of the reference sequence was lower than 80% were discarded. When a contig had multiple hits for the same locus, we selected the best hit (best score obtained by multiplying the coverage by the identity). Detection of the ARGs and plasmids were, respectively, done using the MEGARes database  and the PlasmidFinder database , accessed in April 2018. The MEGARES database was depleted from all genes for which resistance is conferred by SNPs, multi-drug efflux pumps, and regulators. Detection of the class 1, 2, and 3 integron integrase genes was done using the reference sequences AB709942 (intI1), FQ482074 (intI1delta1), JX566770 (intI1R32_N39 aa329337 mutated + 35aa), JX469830 (intI2), and EF467661(intI3) .
Linking plasmid, ARG, and integron contigs to genome clusters
We used a simple heuristic to infer linkages between genome clusters and contigs thought to carry ARGs or plasmid sequences.
For each sample, we considered all Hi-C linkages between contigs of interest and any other contig. We then discarded all contig-cluster linkages represented by only one or two reads. We next used the same criteria to infer all linkages between ARG, integron and plasmid contigs. Phylogenetic analysis of the genomes linked to contigs of interest was performed using the CheckM tree workflow, ape v5.1  and phytools v.0.6-44 . We normalized the number of Hi-C contacts (reads) according to abundance of the plasmid/integron/ARG and abundance of the genome cluster. We discarded contacts <0.01 as spurious. We then summarized all normalized contacts across all contigs corresponding to each plasmid, integron, or ARG family (multiple contigs can correspond to the same plasmid, integron, or ARG family).
Taxonomic summaries of Hi-C linkages with plasmid/ARG/integron contigs
As an alternative measure of plasmid/ARG/integron—host interactions that did not depend on the accuracy of genome clusters, we ignored ProxiMeta clustering results and considered only the set of contigs to which each contig of interest (i.e., plasmids, ARGs, or integrons) is connected by Hi-C. We used BLAST to search these contigs, considering only the best hit among hits with high confidence (E-value <1e−20). We mapped these hits to NCBI taxonomic identifier (taxids) and species names where possible, otherwise recording “no hit” as the species name. We then filtered out contigs for which the coverage of the alignment was <80%, contigs for which there was “no hit”, and contigs identified as “uncultured” or “Candidatus” bacteria. Finally, we counted the number of Hi-C links to each species name, correcting for contig abundance. Where measured abundance was zero, we replaced it with a small nonzero number (0.1 transcript per million (TPMs)).
Results and discussion
Proximity-ligation reconstructs a known plasmid-host association from a wastewater sample
We first validated the ability of Hi-C to assemble the genome of a completely sequenced plasmid-bearing bacterium from a wastewater metagenome. To a portion of a wastewater sample, we added ~7 × 107 CFU/mL of E. coli K12::gfp containing the multi-drug resistance plasmid pB10::rfp, hereafter named EC, which represented ~10% of the total bacterial community (the raw sample is designated WW, the spiked sample WWEC). For both the inoculated and uninoculated samples we generated short-read metagenome assemblies and used ProxiMeta Hi-C deconvolution  to cluster the metagenomic contigs into putative MAGs. This yielded >1000 clusters of contigs for each sample, of which 51 (WW) and 38 (WWEC) were >80% complete bacterial MAGs, as measured by CheckM  (Fig. S1, Tables S1 and S2). In this paper, we use the term “cluster” to describe a cohesive group of contigs belonging to a genome of a microorganism. The EC genome was represented by one large cluster (4.2 Mbp) and three small ones (480 Kbp total) with similar high abundance, producing a >97% complete E. coli genome (Fig. S2a, Table S3). Furthermore, Hi-C linkage between pB10::rfp and its host was extremely strong relative to other clusters (Fig. S2b), confirming that Hi-C can accurately ascertain plasmid-host relationships within a natural diverse microbial community.
Cultivation-independent identification of host-ARG associations
We investigated if Hi-C links could be used to identify the hosts in the WW and WWEC samples that contain DNA sequences with high similarity to previously described ARGs, integrons, and plasmids, using well-known databases [29, 30] (Tables S4 and S5). In addition, we inferred phylogenomic placements of each cluster. We then computed the Hi-C linkage of each ARG-bearing or plasmid-bearing contig to each cluster. The results are presented in Figs. 2–4 for WW, and Figs. S3–S5 for WWEC. Most of the analyses described below are results found to be similar in both WW and WWEC, and if not, this is explicitly stated. ARGs were mostly linked to contigs in clusters related to the Gamma- and Betaproteobacteria (Figs. 2 and S3). The other links to ARGs were mostly associated with clusters affiliated with the Bacteroidetes (Figs. 3 and S4) and the Firmicutes (Figs. 4 and S5), and very few with clusters affiliated with Actinobacteria or Fusobacteria (data not shown). Firmicutes showed reproducible linkages to the ARGs ant9 (linked to Lachnospiraceae), tetO (linked to Lachnospiraceae), ermB and ermG (linked to Streptococacceae), mefA and mefB (linked to Proteocatella sp.), cat and lnuC. No single Firmicutes family or genus was a predominant host for all of these ARGs but clusters were spread out over the phylum and did not point to a specific reservoir of ARGs.
Reproducible linkages observed in both water samples pointed to specific candidate reservoirs that are well-known carriers of these ARGs. For example, clusters related to Prevotella and Bacteroides, two genera within the Bacteroidetes abundantly found in gut microbiota, were linked to tetQ, ermG, mefA, blaCFX, and blaCBLA, which confer resistance to tetracycline, macrolides/lincosamides/streptogramins (MLS), and beta-lactams, respectively, and are commonly found in these genera (Fig. 3) [34,35,36,37]. Specifically tetQ and ermG or ermF are frequently found in tetracycline and erythromycin resistant Bacteroides isolates, respectively (ermF was only detected in the WW sample) [34, 36]. We also found links with genes encoding the Class A betalactamases Cfx CblA, and CepA (the latter only in WW), which are naturally found in members of Bacteroides and Prevotella [35, 37, 38].
Similarly, the ARGs mphE and tet39, recently described on a Acinetobacter baumannii plasmid , were widespread in several clusters affiliated with the genus Acinetobacter (Fig. 2b). Moreover, an ant3” resistance gene and a class 3 integron marker were linked with a cluster related an Acinetobacter johnsonnii, a species recently described to carry these same genetic elements in a wastewater isolate . In addition, several aminoglycoside resistance genes (aac3, aph3”, and aph6), beta-lactamase genes (blaCARB and blaOXA variants), tetracycline resistance gene (tetH), and phenicol resistances gene (floR) were reproducibly associated with the Moraxellaceae.
Interestingly, the bacterial taxa that had the most contacts with known ARGs were affiliated with the Aeromonadaceae (Fig. 2a), a family typically associated with aquatic environments. In WW and WWEC, Aeromonadaceae were linked to no fewer than 18 and 21 ARGs, respectively (14 being in common), conferring resistance to eight antibiotic classes. Similarly, the contigs linked to clusters of the Enterobacteriaceae showed contacts with 18 ARGs in WW and 29 ARGs in WWEC. However, most of these are typically “core structural” genes found in this family, which often only confer reduced susceptibility to antibiotics (i.e., blaEC, pmrA, B,C, F, acrD, E, F, S, pbp4b, pbp2, ampH, arnA, and bacA) . Overall, our results are in line with current knowledge of the taxonomic distribution of the detected ARGs. Hi-C thus suggests that Aeromonadaceae, Moraxellaceae, and Bacteroidetes are the most likely reservoirs of ARGs in this wastewater, consistent with what was suggested in a recent study .
Cultivation-independent identification of host-plasmid associations
Next, we compared the in situ host range of broad-host-range (BHR) and narrow-host-range (NHR) plasmids (Fig. 2c, d). The results were strikingly consistent with our expectations, as two known groups of BHR plasmids (IncQ-1 and IncQ-2) were linked to clusters spanning both Beta- and Gammaproteobacteria, specifically the Enterobacteriaceae, Aeromonadaceae, Neisseriaceae, Rhodocyclaceae, and Comamomadaceae. IncP-1β plasmids, also known to be BHR plasmids were widespread in the WW sample but limited to the Betaproteobacteria. In WWEC, the marker for IncP1-β plasmids was only associated with the EC cluster, possibly because the links to inoculated IncP1-β plasmid pB10::rfp overwhelmed the analysis (Fig. S3). In contrast, markers for several NHR plasmids were almost exclusively linked to clusters belonging to the Enterobacteriaceae. Only one contig with a marker for an IncFIB plasmid was found in a Betaproteobacteria cluster, but the link was ~100 times weaker than with the Enterobacteriaceae. Another striking finding was the linkage of IncQ and IncU plasmids and a colE-type plasmid with the Aeromonadaceae. IncU plamids were originally described in Aeromonas sp. , and the colE-type plasmid was similar to those described in this genus . Moreover, in WWEC a marker for the IncA/C plasmids was also detected in this family (Fig. S3). IncA/C plasmid markers were also detected in the WW sample but no links to clusters were detected. Just like the IncU plasmids, IncA/C plasmids were first described in an Aeromonas species . Further in line with the literature, plasmids previously found in cultured Gram-positives were associated with the Gram-positive family of the Eubacteriaceae in this WW (Fig. 4). We show here that the Hi-C method allows assessing the in situ plasmid-host range without any cultivation steps. IncQ plasmids had the broadest range of putative hosts, followed by the IncP-1β plasmids. This is consistent with the results of studies based on cultured bacteria .
The method was able to identify the Aeromonadaceae as the hosts of the most ARGs as well as several well-known BHR antibiotic resistance plasmids in this wastewater community. This strongly suggests that this family is involved in the spread of antibiotic resistance in WWTP. Aeromonadaceae are ubiquitous in aquatic environment, they are frequently isolated from freshwater, estuarine or wastewater, and several species are pathogenic for humans or other vertebrate and invertebrate . We postulate that Aeromonadaceae may be an important vector of spread of antibiotic resistance in WWTP. On the other hand, clusters related to the Enterobacteriaceae showed links with many plasmids (Fig. 2c), though this may be due to the bias in the plasmid database we used . Although we detected NHR plasmids in this family that are typically involved in antibiotic resistance spread, there were very few links of Enterobacteriaceae clusters with ARGs, most of which showed homology to chromosomally and not plasmid encoded genes. This suggests that in this particular community, the Enterobacteriaceae were not the main vectors of antibiotic resistance spread.
Finally, in the WW sample, one Comamonadaceae cluster with high genome completeness (88.4%; cluster.20) showed strong linkages to an IncP-1β plasmid, a well-known host-plasmid association [48, 49]. We were able to reconstruct two large fragments (22.7 kb and 12.9 kb) carrying the typically conserved transfer regions of IncP1-β plasmids and genes of the maintenance/control region. The closest relative was plasmid pALIDE02 of a WWTP Comamonadaceae isolate (see SI, “Intracellular association of ARGs in cluster.20” and Fig. S8).
Cultivation-independent identification of host-integron associations
Among the integrons, class 1 integrons exhibited links to 39 clusters within the Beta- and Gammaproteobacteria in WW, making it the marker with the broadest host range in this study (Fig. 2). In WWEC no integrons other than the one found in EC were detected, possibly because the strong Hi-C signal of the class 1 integron integrase gene from pB10::rfp carried by EC (Fig S3). Class 2 and 3 integrons were associated with clusters affiliated with the Neisseriaceae (Fig. 2e). Whereas class 2 integrons were previously found in a Neisseria sp. WWTP isolate (Accession numbers FJ502342 and FJ502343), class 3 integrons were described in a Delftia sp. WWTP isolate , but so far never in Neisseriaceae (Integrall database, http://integrall.bio.ua.pt, consulted on 14 February 2019). This may thus be the first description of class 3 integrons in Neisseriaceae.
We conclude that the Hi-C links can help determine the taxonomic placement of the hosts of ARGs and mobile elements in an environmental habitat despite some methodological challenges that will need to be addressed in future work. We describe these challenges below.
Estimation of the relative abundance of host-marker associations
Like other metagenomics approaches, proximity ligation can only detect associations above some limit of detection imposed by sample complexity and sequencing depth. We attempted to estimate an upper bound on the detection limit of our method for our samples to inform future work applying this technology. This was done by using the abundance of pB10::rfp in WWEC (determined by shotgun read coverage) and the input fraction of its host EC (~10%) to roughly calibrate the relative abundance of hosts linked to other markers (e.g., ARGs or plasmids) (Table S6). Examining several ARGs and plasmid families recapitulating known host relationships, we estimated that these relative host abundances ranged from 0.001% (colE-type plasmids) to 0.5% (tet39). While we infer host associations for many elements at the low end of this range, we suggest that a relative host abundance of 0.01% is a conservative estimate of the limit of detection of our method. In other words, we are able to detect host-plasmid associations which are present in one out of 10,000 cells in the sample under the conditions used in this study. This estimate is only an upper bound, and we expect that with further development this detection limit could be lowered. Further validation will require experimental determination of the limit of detection, and also the isolation of specific culturable bacteria to verify their ARGs and plasmid content.
Limitations of Hi-C
A first limitation we identified is that highly abundant genomes may produce some clustering artifacts. In WWEC, several clusters had Hi-C linkages to chromosomal genes of the EC that was added to the sample (arrows on Figs. S3 and S5). One of these cluster was EC, as expected, but the other clusters were related to the Firmicutes, Alpha- or Betaproteobacteria. The presence of these EC genes in such taxa is very unlikely. We conclude that these Hi-C linkages detected in the WWEC were spurious links probably related to the high abundance of the spiked organism. We also observed Hi-C links between the EC cluster and ARGs or plasmid markers that do not belong to the genome of the spiked strain (i.e., IncFIB, IncFII, and col plasmids, and several ARGs). Such plasmids and ARGs were likely present in other strains of E. coli or closely related species present in the wastewater. Indeed, several clusters related to Escherichia sp. but different from our strain EC were detected in the WW sample (Fig. 2), some of which had Hi-C links to these genes. Similar observations were made for a cluster that shared some contigs closely related to E. coli, and for the cluster.20 described above containing an IncP1-β plasmid very similar to the plasmid pB10::rfp spiked with EC (see details in caption of Figs. S5 and S8, respectively). We therefore conclude that the resolution of contigs that are shared between closely related bacterial species or plasmids may produce spurious links, making it difficult to pinpoint the linkage of a marker to a specific strain. These limitations can be addressed in the future by further optimizing abundance normalization schemes such as the ones we employed in this study, though these require accurate abundance estimates.
Clustering-independent identification of plasmid and ARG hosts
It is possible that ProxiMeta has misclustered contigs, giving us chimeric clusters composed of contigs from different microorganisms. Moreover, the taxonomic assignment done by the lineage-specific marker sets approach used in CheckM may not always have been accurate. We therefore verified the accuracy of our plasmid/integron/ARG host assignments independently of the clusters assembled by ProxiMeta and the taxonomic affiliation inferred by CheckM. We did this by performing taxonomic profiling of all the contigs that were linked to contigs of plasmids, integrons, or ARGs. We first selected these plasmid/integron/ARG containing contigs and then isolated all the contigs linked to these contigs by at least one Hi-C link. Then we used BLAST to find matches of each such linked contig against the NCBI bacterial database (see the “Materials and methods” section). We summarized the host taxonomy of the contigs linked to plasmid/integron/ARG markers in Fig. 5. The new taxonomic affiliation obtained this way were mostly similar to those identified by ProxiMeta (Fig. 2). For example, the MLS resistance gene mphE was strongly associated with contigs related to both Gamma- and Betaproteobacteria, more specifically the Moraxellaceae and Neisseriaceae. Moreover, both quinolone resistance genes qnrS and qnrVC were mainly associated with contigs of the Gammaproteobacteria, mostly the Aeromonadaceae. As in our previous approach, markers for BHR plasmids were linked to clusters spanning the Beta- and Gammaproteobacteria while markers for NHR plasmids were strongly linked to clusters that only belonged mostly to the Enterobacteriaceae. Only the phylogenetic attribution of the ARG mefA was different between our two approaches.
While several questions about the accuracy and sensitivity of this approach remain, we show that in vivo Proximity-ligation can help assess the in situ host range of ARGs, plasmids, and integrons in a natural microbial community. We determined for the first-time using cultivation-independent method that IncQ plasmids and class 1 integrons had the broadest host range, confirming these genetic elements as important vectors for horizontal gene transfer. We also concluded that bacteria belonging to the Aeromonadaceae, Moraxellaceae, and Bacteroidetes are important reservoirs of antibiotic resistance in WWTP, and argued that Aeromonadaceae may play a critical role in the spread of antibiotic resistance in WWTP. These analyses can be easily expanded to other MGEs and other habitats. This novel approach fills an important gap in our ability to track the reservoirs and horizontal transfer of antibiotic resistance genes, with the ultimate goal of slowing down the spread of drug resistance.
Sequencing data are available in FASTQ format at SRA accession PRJNA506462. Processed data and scripts for linking contigs to genome clusters using Hi-C data are available at https://osf.io/ezb8j/.
Klemm EJ, Shakoor S, Page AJ, Qamar FN, Judge K, Saeed DK, et al. Emergence of an extensively drug-resistant Salmonella enterica serovar Typhi clone harboring a promiscuous plasmid encoding resistance to fluoroquinolones and third-generation cephalosporins. MBio. 2018;9:e00105–18.
Krapp F, Ozer EA, Qi C, Hauser AR. Case report of an extensively drug-resistant Klebsiella pneumoniae infection with genomic characterization of the strain and review of similar cases in the United States. Open Forum Infect Dis. 2018;5:ofy074.
Man TJBde, Lutgring JD, Lonsway DR, Anderson KF, Kiehlbauch JA, Chen L, et al. Genomic analysis of a pan-resistant isolate of Klebsiella pneumoniae, United States 2016. mBio. 2018;9:e00440–18.
Martínez-Martínez L, Pascual A, Jacoby GA. Quinolone resistance from a transferable plasmid. Lancet. 1998;351:797–9.
Yigit H, Queenan AM, Anderson GJ, Domenech-Sanchez A, Biddle JW, Steward CD, et al. Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother. 2001;45:1151–61.
Liu Y-Y, Wang Y, Walsh TR, Yi L-X, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16:161–8.
Mazel D. Integrons: agents of bacterial evolution. Nat Rev Microbiol. 2006;4:608–20.
Berendonk TU, Manaia CM, Merlin C, Fatta-Kassinos D, Cytryn E, Walsh F, et al. Tackling antibiotic resistance: the environmental framework. Nat Rev Microbiol. 2015;13:310–7.
Martínez JL, Coque TM, Baquero F. What is a resistance gene? Ranking risk in resistomes. Nat Rev Microbiol. 2015;13:116–23.
Surette M, Wright GD Lessons from the environmental antibiotic resistome. Annu Rev Microbiol. 2017;71:309–29.
Beitel CW, Froenicke L, Lang JM, Korf IF, Michelmore RW, Eisen JA, et al. Strain- and plasmid-level deconvolution of a synthetic metagenome by sequencing proximity ligation products. PeerJ. 2014;2:e415.
Burton JN, Liachko I, Dunham MJ, Shendure J. Species-level deconvolution of metagenome assemblies with Hi-C-based contact probability maps. G3 (Bethesda). 2014;4:1339–46.
Marbouty M, Cournac A, Flot J-F, Marie-Nelly H, Mozziconacci J, Koszul R. Metagenomic chromosome conformation capture (meta3C) unveils the diversity of chromosome organization in microorganisms. Elife. 2014;3:e03318.
Marbouty M, Baudry L, Cournac A, Koszul R. Scaffolding bacterial genomes and probing host-virus interactions in gut microbiome by proximity ligation (chromosome capture) assay. Sci Adv. 2017;3:e1602105.
Press MO, Wiser AH, Kronenberg ZN, Langford KW, Shakya M, Lo C-C, et al. Hi-C deconvolution of a human gut microbiome yields high-quality draft genomes and reveals plasmid-genome interactions. bioRxiv 2017; 198713. https://doi.org/10.1101/198713.
Stewart RD, Auffret MD, Warr A, Wiser AH, Press MO, Langford KW, et al. Assembly of 913 microbial genomes from metagenomic sequencing of the cow rumen. Nat Commun. 2018;9:870.
Fox RE, Zhong X, Krone SM, Top EM. Spatial structure and nutrients promote invasion of IncP-1 plasmids in bacterial populations. ISME J. 2008;2:1024–39.
Liu CM, Aziz M, Kachur S, Hsueh P-R, Huang Y-T, Keim P, et al. BactQuant: an enhanced broad-coverage bacterial quantitative real-time PCR assay. BMC Microbiol. 2012;12:56.
Li D, Liu C-M, Luo R, Sadakane K, Lam T-W. MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics. 2015;31:1674–6.
Mikheenko A, Saveliev V, Gurevich A. MetaQUAST: evaluation of metagenome assemblies. Bioinformatics. 2016;32:1088–90.
Li H, Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009;25:1754–60.
Li H Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. arXiv 1303.3997v2. 2013.
Ondov BD, Treangen TJ, Melsted P, Mallonee AB, Bergman NH, Koren S, et al. Mash: fast genome and metagenome distance estimation using MinHash. Genome Biol. 2016;17:132.
Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res. 2015;25:1043–55. gr.186072.114.
Hausser J, Strimmer K. Entropy inference and the James-Stein estimator, with application to nonlinear gene association networks. arXiv 0811.3579v3. 2008.
Schaeffer L, Pimentel H, Bray N, Melsted P, Pachter L. Pseudoalignment for metagenomic read assignment. Bioinformatics. 2017;33:2082–8.
Darzentas N. Circoletto: visualizing sequence similarity with Circos. Bioinformatics. 2010;26:2620–1.
Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics. 2013;29:1072–5.
Lakin SM, Dean C, Noyes NR, Dettenwanger A, Ross AS, Doster E, et al. MEGARes: an antimicrobial resistance database for high throughput sequencing. Nucleic Acids Res. 2017;45:D574–D580.
Carattoli A, Zankari E, García-Fernández A, Voldby Larsen M, Lund O, Villa L, et al. In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother. 2014;58:3895–903.
Moura A, Soares M, Pereira C, Leitão N, Henriques I, Correia A. INTEGRALL: a database and search engine for integrons, integrases and gene cassettes. Bioinformatics. 2009;25:1096–8.
Paradis E, Claude J, Strimmer K. APE: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004;20:289–90.
Revell LJ. phytools: an R package for phylogenetic comparative biology (and other things): phytools: R package. Methods Ecol Evol. 2012;3:217–23.
Eitel Z, Sóki J, Urbán E, Nagy E. ESCMID Study Group on Anaerobic Infection. The prevalence of antibiotic resistance genes in Bacteroides fragilis group strains isolated in different European countries. Anaerobe. 2013;21:43–9.
Binta B, Patel M. Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes. J Appl Oral Sci. 2016;24:142–7.
Shoemaker NB, Vlamakis H, Hayes K, Salyers AA. Evidence for extensive resistance gene transfer among Bacteroides spp. and among Bacteroides and other genera in the human colon. Appl Environ Microbiol. 2001;67:561–8.
Giraud-Morin C, Madinier I, Fosse T. Sequence analysis of cfxA2-like beta-lactamases in Prevotella species. J Antimicrob Chemother. 2003;51:1293–6.
Philippon A, Slama P, Dény P, Labia R. A structure-based classification of class A β-lactamases, a broadly diverse family of enzymes. Clin Microbiol Rev. 2016;29:29–57.
Blackwell GA, Hall RM. The tet39 determinant and the msrE-mphE genes in Acinetobacter plasmids are each part of discrete modules flanked by inversely oriented pdif (XerC-XerD) sites. Antimicrob Agents Chemother. 2017;61:e00780–17.
Simo Tchuinte PL, Stalder T, Venditti S, Ngandjio A, Dagot C, Ploy M-C, et al. Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg. Int J Antimicrob Agents. 2016;48:431–4.
Vikram A, Rovira P, Agga GE, Arthur TM, Bosilevac JM, Wheeler TL, et al. Impact of “raised without antibiotics” beef cattle production practices on occurrences of antimicrobial resistance. Appl Environ Microbiol. 2017;83:e01682–17.
Narciso-da-Rocha C, Rocha J, Vaz-Moreira I, Lira F, Tamames J, Henriques I, et al. Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant. Environ Int. 2018;118:179–88.
Sørum H, L’Abée-Lund TM, Solberg A, Wold A. Integron-containing IncU R plasmids pRAS1 and pAr32 from the fish pathogen Aeromonas salmonicida. Antimicrob Agents Chemother. 2003;47:1285–90.
Potron A, Nordmann P, Lafeuille E, Al Maskari Z, Al Rashdi F, Poirel L. Characterization of OXA-181, a carbapenem-hydrolyzing class D beta-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2011;55:4896–9.
Johnson TJ, Lang KS. IncA/C plasmids. Mob Genet Elements. 2012;2:55–58.
Suzuki H, Yano H, Brown CJ, Top EM. Predicting plasmid promiscuity based on genomic signature. J Bacteriol. 2010;192:6045–55.
Brenner DJ, Garrity GM, Bergey DH (eds). The Gammaproteobacteria, 2nd edn. New York, NY: Springer; 2005.
Krol JE, Penrod JT, McCaslin H, Rogers LM, Yano H, Stancik AD, et al. Role of IncP-1 plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses. Appl Environ Microbiol. 2011;78:828–38.
Norberg P, Bergström M, Jethava V, Dubhashi D, Hermansson M. The IncP-1 plasmid backbone adapts to different host bacterial species and evolves through homologous recombination. Nat Commun. 2011;2:268.
Xu H, Davies J, Miao V. Molecular characterization of class 3 integrons from Delftia spp. J Bacteriol. 2007;189:6276–83.
Research reported in this publication was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P30 GM103324 through an IBEST Pilot Grant, and in part by grants 2R44AI122654-02A1 and 1R43AI122654-01 to Phase Genomics. We thank the IBEST Genomics Resources Core staff Dan New, Matt Fagnan, and Sam Hunter for assistance with shotgun library preparations and sequencing, and Kathryn Mueller for help with Fig. 1 drawing.
Conflict of interest
MP, SS, and IL are employees and shareholders of Phase Genomics, Inc—a company commercializing proximity-ligation technology. IL and SS are executives at Phase Genomics, Inc.
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Stalder, T., Press, M.O., Sullivan, S. et al. Linking the resistome and plasmidome to the microbiome. ISME J 13, 2437–2446 (2019) doi:10.1038/s41396-019-0446-4