We are living in a microbial world with our bodies having as many microbial cells as human cells. Growing evidence implicates these microbes, known collectively as the microbiome, as key regulators of brain function and behaviour . One of the key findings from across many species is that the microbiome affects social behaviour . We have shown that germ-free (GF) mice, which grow up in a sterile environment and thus have no bacteria in or on their bodies, are less sociable than normal mice. Moreover, the amygdala, a brain region important for social behaviour, is particularly sensitive to changes in microbiome composition  and GF mice have widespread changes in amygdala neuronal morphology and function .
Ongoing research is trying to determine the molecular mechanisms underpinning such effects. Initially, we exploited unbiased genome-wide transcriptional profiling to determine gene expression in the amygdala of male GF mice. We found differential gene expression, exon usage and RNA-editing in GF mice (Fig. 1). We noticed upregulation of several immediate early response genes such as Fos, Fosb, Egr2 or Nr4a1 in association with increased cAMP response element-binding protein (CREB) signalling in GF mice . In addition, we found differential expression and recoding of several genes implicated in a variety of neuronal processes such as neurotransmission, neuronal plasticity, metabolism and morphology. These data strongly suggest altered baseline neuronal activity in the amygdala of GF animals, which may underpin the social deficits. However, what happens under a social stimulus remained known.
To this end we recently described dynamic regulation of several previously undescribed pathways in response to social stimulation. These include regulation of RNA-processing non-coding RNAs that are crucially involved in splicing regulation. Moreover, social stimulus evoked an increase in transcripts of genes involved in neuronal activity, which includes induction of several well established immediate early genes such as Fos or Arc, the MAP-K pathway and neurotrophic signalling via Bdnf. Moreover, we find upregulation of complement components, which have lately been established to be necessary for synaptic rearrangements and plasticity upon neuronal activity .
However, GF mice displayed a strikingly different pattern of amygdala gene activity in response to social interaction  (Fig. 1). In particular, the dynamic, stimulus-dependent transcriptional regulation seen in controls was attenuated and replaced by a marked increase in expression of splicing factors and alternative exon usage. This reveals a potential molecular basis for how the host microbiome is crucial for a normal behavioural response during social interaction. Moreover, social behaviour was correlated with the amygdala gene-expression response. These results reveal one of the key steps leading from absence of bacteria during brain development to a phenotype associated with reduced sociability in adulthood in mice. These data thus enhance our understanding of the link between the microbiome and brain health and neurodevelopmental disorders such as autism spectrum disorders. Future studies will be needed to determine what are the exact microbial signals that regulate alternative splicing events in the amygdala and whether they can be harnessed for therapeutic benefit.
Funding and Disclosure
The authors are supported by Science Foundation Ireland (Grant Nos. SFI/12/RC/2273). They have had research support from Mead Johnson, Cremo, 4D Pharma, Suntory Wellness, Dupont and Nutricia.
Allen AP, Dinan TG, Clarke G, Cryan JF. A psychology of the human brain-gut-microbiome axis. Soc Personal Psychol Compass. 2017;11:e12309.
Desbonnet L, Clarke G, Shanahan F, Dinan TG, Cryan JF. Microbiota is essential for social development in the mouse. Mol Psychiatry. 2014;19:146–8.
Cowan CSM, Hoban AE, Ventura-Silva AP, Dinan TG, Clarke G, Cryan JF. Gutsy moves: the amygdala as a critical node in microbiota to brain signaling. Bioessays. 2018;40:1700172.
Luczynski P, McVey Neufeld KA, Oriach CS, Clarke G, Dinan TG, Cryan JF. Growing up in a bubble: using germ-free animals to assess the influence of the gut microbiota on brain and behavior. Int J Neuropsychopharmacol. 2016;19:pii: pyw020.
Stilling RM, Ryan FJ, Hoban AE, Shanahan F, Clarke G, Claesson MJ, et al. Microbes & neurodevelopment—absence of microbiota during early life increases activity-related transcriptional pathways in the amygdala. Brain Behav Immun. 2015;50:209–20.
Stilling RM, Moloney GM, Ryan FJ, Hoban AE, Bastiaanssen TF, Shanahan F, et al. Social interaction-induced activation of RNA splicing in the amygdala of microbiome-deficient mice. eLife. 2018;7:pii: e33070.
The authors would like to thank Dr. Roman Stilling for his stimulating insights into amygdala-microbiome interactions that form the basis of this short article and Dr. Stilling and Dr. Kiran Sandhu for assistance with Fig. 1.
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Cryan, J.F., Dinan, T.G. Decoding the role of the microbiome on amygdala function and social behaviour. Neuropsychopharmacol 44, 233–234 (2019). https://doi.org/10.1038/s41386-018-0233-3
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