Parasitic helminths are unique pathogens, being the largest, most chronic, and yet often the least pathogenic infectious agents that we encounter. They travel across and persist in many mucosal sites, and their impact, experimentally and clinically, has supported many of our current models of mucosal immunity. Here, we present a special collection of recent Mucosal Immunology articles highlighting key advances made possible through the study of helminth infections, from the discovery of new immuno-epithelial cells to the cross-kingdom interactions that govern intestinal immunity and the therapeutic promise of helminth-mediated immune suppression. Together these articles are a celebration of the power of the worm to provide new insight into the mechanisms of mucosal immunity, and we hope you enjoy reading the collection.
Lessons from helminths began very early in history. Parasitic helminths are visible to the naked eye and were the first pathogens to be described, with the earliest known medical text, the Ebers papyrus (~1500 BC), describing human tapeworm, roundworm, schistosome, hookworm and guinea worm infections1. Helminths have long been associated with type 2 immune responses, many aspects of which were uncovered using experimental infections (Fig. 1). The first to be identified were eosinophils and mast cells, discovered in the late 1800s by Paul Ehrlich2 and later shown to be associated with parasitic worm infections3,4 (Fig. 1-1). Likewise, early helminth infection studies led Bridget Ogilvie and her team to describe “Reginin-like antibodies” (later renamed IgE) in 19641 (Fig. 1-2). When Mosmann and Coffman originally proposed the Th1/Th2 paradigm, this was supported by the identification of Th2-phenotype cells in helminth-infected mice5 (Fig. 1-3), while the impact of the Th2 signature cytokines was demonstrated in helminth infections of early IL-4 and IL-13 knockout animals6,7,8. Initially, IL-4 and IL-13 were thought to fulfil redundant roles in the immune response, until helminth infection studies revealed the dominant role of IL-13 in whipworm expulsion9. IL-13 was later shown to be a critical cytokine at mucosal surfaces, activating the “epithelial escalator” (increased epithelial turnover)10 and a key part of the “weep and sweep” response (coordinated increases in epithelial permeability, mucus production and intestinal motility) that clears intestinal parasites11 (Fig. 1-6). The first experimental data supporting the mucus layer as a key part of mucosal immunity was also provided by experimental helminth infections in the early 1980s12. More recently, the first studies on the alternative activation of macrophages13 (Fig. 1-5), and the concept of wound healing as an immune outcome14, were also built on helminth infection experiments. Such infections have also shown that tissue macrophages proliferate in situ15 (Fig. 1-8) and that T follicular helper cells are functionally polarised16,17. Finally, the identification and characterisation of 2 of the critical initiators and regulators of type 2 immunity—type 2 innate lymphoid cells (ILC2s)18 (Fig. 1-7) and tuft cells19 (Fig. 1-10)—both depended on the use of helminth infections.
Today, the contribution of helminths to fundamental understanding of mucosal immunology continues at pace. Two research articles in this collection describe the dialogue between tuft cells and ILC2s: first showing that the cytokine MIF is required for tuft cell and ILC2 activation, critical for rapid expulsion of intestinal helminths20, and second that the SOCS-family member CISH regulates that tuft cell-ILC2 circuit, controlling the thresholds of epithelial and immune cell interaction21. In a review article in this issue, Inclan-Rico et al. discuss current understanding of cell-cell interactions in the infected mucosa, highlighting the function of non-traditional immune cells such as epithelial cells, neurons and fibroblasts, and arguing that communication between haematopoietic, stromal and neural cells is essential not only for the initiation of mucosal immunity, but also for its amplification, regulation and repair22.
The theme of resolution and repair is continued in a study showing that the phosphatase PTPN2 is a critical signalling step in the alternative activation of macrophages, and its absence correlates with excessive lung damage in a model of pulmonary helminth infection23. Type 2 immunity (including macrophage alternative activation) is associated with distinct metabolic requirements, favouring oxidative phosphorylation over aerobic glycolysis and these metabolic changes can have both dietary and immunological determinants (Fig. 1-9). Our collection includes an intriguing new study of ILC activation in the intestine that demonstrates that a neuropeptide elicited by food consumption, VIP, synergises with cytokine alarmins to potently activate ILC2s and ILC3s in the intestinal tissue24. In a new review in this issue, Michla et al. focuses on cutting-edge ILC2 biology and particularly the metabolic pressures that govern ILC activity and consequent immune decisions25.
One of the features of mucosal sites is constant interaction with external stimuli, such as food, dust, and commensal microorganisms. Mucosal infections take place in the context of this background stimulation, and helminth infections have provided fascinating new insight into the interactions that take place. A recent paper in our collection shows that the bacterial microbiota also influences helminth expulsion by regulating intestinal contractility26. Cross-regulation also occurs via the immune system, and two new papers highlight the impact of interaction between opposing cytokine responses. Both describe an underlying IFNγ response present during helminth infection that limits the Th2 response critical for parasite expulsion27,28, and IL-10 is shown to be critical for keeping that IFNγ in check28.
The immune regulation revealed by helminth infection is concentrated at the site of infection, but these infections have also revealed long-range immune modulation, driving our understanding of communication between distant immune locations. Helminths played a key role in describing the gut-lung axis, including the early demonstration that intestinal infection can alter pulmonary disease. Connections between other distal sites are now being established and an exciting new paper in our collection reveals profound changes in immune populations in the skin during strictly enteric helminth infection29. Both the tissue specificity of immune responses and the connections between distant locations are explored in a new review by Vacca et al. in this issue30, highlighting the long reach of helminth infections.
The mechanisms of immune regulation revealed by helminth infections have also been powerful drivers of therapeutic strategies, aiming to suppress and moderate pathological immune responses in a variety of tissue sites. Soon after the discovery of regulatory T cells31, parasites were found to induce regulatory responses and suppress type 2 immunity, through the release of immunomodulatory products32 (Fig. 1-4). Loke et al. review the latest information on the human immune response to parasitic helminths, how recent technological developments such as human challenge studies, single cell sequencing and organoid culture systems have aided these investigations. Recent insight is enabling new approaches to vaccine design and harnessing of parasite-mediated immune suppression to treat inflammatory disease33.
Parasitic helminths will continue to be a unique and physiologically relevant testbed for new concepts in mucosal immunology. Better understanding of immunity to helminths is urgently needed. Almost one quarter of the world’s population is at risk of infection with parasitic helminths, causing significant morbidity and mortality, and yet we currently do not have any effective vaccines. But as this collection of reviews and current research papers illustrates, understanding the biology of helminth infections, their regulation and their tissue context is also answering key questions in immunobiology, mucosal immunity, and immunotherapy. Only by using human and animal model helminth infection experiments, can these fundamental questions be answered.
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Perona-Wright, G., McSorley, H.J. Lessons from helminths: what worms have taught us about mucosal immunology. Mucosal Immunol 15, 1049–1051 (2022). https://doi.org/10.1038/s41385-022-00560-5
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DOI: https://doi.org/10.1038/s41385-022-00560-5