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Restoring glutamate receptosome dynamics at synapses rescues autism-like deficits in Shank3-deficient mice

Molecular Psychiatry volume 26, pages 7596–7609 (2021)Cite this article

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Abstract

Shank3 monogenic mutations lead to autism spectrum disorders (ASD). Shank3 is part of the glutamate receptosome that physically links ionotropic NMDA receptors to metabotropic mGlu5 receptors through interactions with scaffolding proteins PSD95–GKAP–Shank3–Homer. A main physiological function of the glutamate receptosome is to control NMDA synaptic function that is required for plasticity induction. Intact glutamate receptosome supports glutamate receptors activation and plasticity induction, while glutamate receptosome disruption blocks receptors activity, preventing the induction of subsequent plasticity. Despite possible impact on metaplasticity and cognitive behaviors, scaffold interaction dynamics and their consequences are poorly defined. Here, we used mGlu5–Homer interaction as a biosensor of glutamate receptosome integrity to report changes in synapse availability for plasticity induction. Combining BRET imaging and electrophysiology, we show that a transient neuronal depolarization inducing NMDA-dependent plasticity disrupts glutamate receptosome in a long-lasting manner at synapses and activates signaling pathways required for the expression of the initiated neuronal plasticity, such as ERK and mTOR pathways. Glutamate receptosome disruption also decreases the NMDA/AMPA ratio, freezing the sensitivity of the synapse to subsequent changes of neuronal activity. These data show the importance of a fine-tuning of protein–protein interactions within glutamate receptosome, driven by changes of neuronal activity, to control plasticity. In a mouse model of ASD, a truncated mutant form of Shank3 prevents the integrity of the glutamate receptosome. These mice display altered plasticity, anxiety-like, and stereotyped behaviors. Interestingly, repairing the integrity of glutamate receptosome and its sensitivity to the neuronal activity rescued synaptic transmission, plasticity, and some behavioral traits of Shank3∆C mice. Altogether, our findings characterize mechanisms by which Shank3 mutations cause ASD and highlight scaffold dynamics as new therapeutic target.

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Fig. 1: Transient depolarization induces a long-lasting disruption of mGlu5–Homer interaction in spines.
Fig. 2: Acute mGlu5–Homer disruption impairs NMDA/AMPA postsynaptic currents ratio and ERK and mTOR signaling pathways.
Fig. 3: NMDA/AMPA currents ratio, ERK and mTOR signaling pathways, and mGlu5–Homer interaction are altered in Shank3∆C mice.
Fig. 4: Repairing glutamate receptosome dynamics rescues ERK and mTOR signaling pathways in Shank3∆C mice.
Fig. 5: in vivo glutamate receptosome dynamics reestablishment rescues NMDA/AMPA currents and autistic-like behaviors in Shank3∆C mice.

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Acknowledgements

The authors thank the iExplore animal facility (IGF, Montpellier), CompAn behavioral phenotyping facility (MMDN, Montpellier), and the Arpege platform (IGF, Montpellier) for the use of the Infinite F500 plate reader for cell-population BRET. The authors thank Muriel Asari for illustrations. The authors thank Hélène Hirbec for helping with statistical analysis and Giorgio Cignitti for helping with patch on slices. The authors also gratefully acknowledge Paul F. Worley for the generous gift of the Shank3∆C mouse model. The authors truly thank Dr. Amanda Hernan for proofreading. This work was supported by the Agence Nationale de la Recherche (VC, ANR-18-CE16-0011-01) and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (JP, grant agreement No. 646788), ANR Lanthslider (JP, ANR-17-CE11-0046), Comitato Telethon Fondazione Onlus (grant no. GGP16131 to CV and GGP17176 to CS), and Regione Lombardia NeOn Progetto “NeOn” POR-FESR 2014-2020, ID 239047, CUP E47F17000000009 to CS and CV).

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  1. These authors contributed equally: Sophie Sakkaki, Vincent Compan, Nathalie Bouquier.

Authors and Affiliations

  1. IGF, University of Montpellier, CNRS, INSERM, Montpellier, France

    Enora Moutin, Sophie Sakkaki, Vincent Compan, Nathalie Bouquier, Julie Areias, Anne-Laure Hemonnot-Girard, Vincent Seube, Bastien Glasson, Yan Chastagnier, Fabrice Raynaud, Etienne Audinat & Julie Perroy

  2. Cnr Neuroscience Institute, 3220129, Milan, Italy

    Federica Giona, Carlo Sala & Chiara Verpelli

  3. Interdisciplinary Institute for NeuroScience, CNRS, UMR 5297, Centre Broca Nouvelle-Aquitaine, 33076, Bordeaux, France

    Elise Goyet, Nathan Benac & Laurent Groc

  4. PhyMedExp, Univ Montpellier, INSERM, CNRS, CHU de Montpellier, France

    Fabrice Raynaud

  5. MMDN, Univ Montpellier, EPHE, INSERM, Montpellier, France

    Tangui Maurice

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Contributions

EM and JP conceived research and wrote the manuscript; JP, EM, VC, CV, CS, TM, EA, LG, NBo, and SS designed research experiments; VC, NBo, and FG cloned all plasmids. VC and EM produced viruses. EM performed cell-population BRET experiments; EM, JP, VS, and A-LH-G performed microscopy BRET experiments. EG performed single particle training experiments. NBe analyzed single particle training experiments, with the help of EG and LG. Author EM performed electrophysiology in slices, with the help of EA and FR. Author VC performed coimmunoprecipitations with the help of EM and NBo. Authors CV, CS, and FG designed and characterized the Homer–GluN2B construct. EM performed synaptosomes preparations. NBo and EM performed immunohistochemistry on injected brains. SS performed surgery and viral injections. SS, BG, and JA performed behavioral experiments with the help of TM. Author YC developed toolsets for BRET analysis. JP supervised the project. All authors contributed to the preparation of the manuscript and approved it.

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Correspondence to Enora Moutin or Julie Perroy.

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Moutin, E., Sakkaki, S., Compan, V. et al. Restoring glutamate receptosome dynamics at synapses rescues autism-like deficits in Shank3-deficient mice. Mol Psychiatry 26, 7596–7609 (2021). https://doi.org/10.1038/s41380-021-01230-x

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