Abstract
Background/Objectives
This study investigated the functions of CCAAT/enhancer-binding protein zeta (C/EBPZ; Gene ID: 10153) in adipose tissue.
Subjects/methods
Bioinformatics analysis were used to study the expression pattern of C/EBPZ in human adipose tissue. The expression and function of C/EBPZ in adipose tissue were further studied using chicken as animal model in vivo and in vitro.
Results
The human C/EBPZ transcripts were greater and more stable in subcutaneous adipose tissue than in visceral adipose tissue (P < 0.01), and they were increased with age in adipose tissue (P < 0.05). In addition, the chicken C/EBPZ transcripts (C/EBPZ /ACTB) of visceral (abdominal) adipose tissue were significantly different between fat and lean broilers and decreased with age during development (P < 0.01). RNA-seq analysis showed that the C/EBPZ overexpression associated with adipose tissue development and DNA replication in chicken preadipocytes (P < 0.05). Additionally, overexpression of chicken C/EBPZ inhibited preadipocytes differentiation and promoted preadipoytes proliferation in vitro (P < 0.05). In addition, C/EBPZ overexpression suppressed the promoter activities of PPARγ, C/EBPα, FASN and LPL, and promoted the promoter activities of GATA2 and FABP4 in chicken preadipocytes (P < 0.05).
Conclusions
C/EBPZ modulated the differentiation and proliferation of preadipocytes, and it might be a new negative regulator of adipogenesis.
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Introduction
Adipose tissue, a loose connective tissue composed mostly of adipocytes, is an important organ for lipid storage and endocrine in human being and animals [1]. Abnormality in formation of adipose tissue results in obesity, metabolic syndrome and lipodystrophy, and it is associated with type 2 diabetes mellitus (T2DM), cardiovascular disease, nonalcoholic fatty liver disease, cancers, and other several diseases [2].
Adipogenesis is mediated by a series of complex processes, including commitment of mesenchymal stem cells into preadipocytes and differentiation of preadipocytes to mature adipocytes [3]. Transcriptional control plays an important role in adipogenesis [3]. Of transcription factors (TFs), peroxisome proliferator-activated receptor gamma (PPARγ) is undoubtedly the most important transcriptional modulator of adipocyte development in all types of adipose tissue [4]. In addition, CCAAT/enhancer-binding proteins (C/EBPs), Kruppel-like factors (KLFs) and some other TFs play important roles in adipogenesis [4].
CCAAT/enhancer-binding protein zeta (C/EBPZ, Gene ID: 10153); alternative names CBF, CBF-2, NOC1 and HSP-CBF, is a ubiquitous and highly conserved protein in animals. Despite its name it should not be considered as a C/EBP family member because it lacks significant homology with other C/EBP members, and in fact it is not a basic leucine zipper (bZIP) protein [5]. To complicate things even more, DNA damage inducible transcript 3 (DDIT3, Gene ID: 1649) was also named C/EBPZ in several publications [6].
The real C/EBPZ (Gene ID: 10153) was first reported as a CCAAT-box binding transcription factor of human heat-shock protein 70 (HSP70) promoter, and it facilitated HSP70 promoter activity in a CCAAT-dependent manner [7]. Further study showed that C/EBPZ might not directly DNA sequence-specific bind to CCAAT-box but associated with a CCAAT-bound NF-Y in HSP activation, and it could be a protein scaffold that integrates interactions with different transcription factors [8].
C/EBPZ was reported to be a mRNA binding protein [9, 10] and nucleolar protein [11] in human cells. Additionally, C/EBPZ was involved in m6A methylation of RNA by recruiting methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit (METTL3) to transcription start sites (TSSs) of target genes in acute myeloid leukemia (AML) [12].
Genetic study showed that C/EBPZ was a recurrently mutated gene in AML [13] and associated with schizophrenia in human [14]. Additionally, comparative host-coronavirus protein interaction networks revealed that C/EBPZ might take a part in the viral-human protein-protein interaction for SARS-CoV-1, MERS-CoV, and SARS-CoV-2 [15].
DDIT3 (Gene ID: 1649), once named C/EBPZ in several publications, was reported as an important negative regulator in adipogenesis [16], however, there was no report of real C/EBPZ (Gene ID: 10153) on adipogenesis to date. In the current study, the role of C/EBPZ (Gene ID: 10153) in the regulation of adipogenesis was studied, and the results provided an evidence that C/EBPZ was a new negative regulator of adipogenesis.
Materials and methods
Experimental birds and management
All animal experiments were conducted according to the guidelines for care and use of experimental animals established by the Ministry of Science and Technology of the People’s Republic of China (Approval number: 2006-398), and they were approved by the Animal Experimental Ethical Committee of First Affiliated Hospital, Shihezi University School of Medicine (Approval number: A2019-009-01). In brief, several 12-day-old and six 7-week-old male Arbor Acres (AA) broilers were sacrificed under ether anaesthesia for stromal-vascular cells isolation and tissue expression analysis, respectively, and before sacrificed, they all had free access to feed and water.
Tissues
Six male AA birds were slaughtered at 7 weeks of age, 15 tissue samples were collected: including, visceral (abdominal) fat tissue, liver, duodenum, jejunum, ileum, pectoralis, leg muscle, gizzard, heart, spleen, kidney, pancreas, proventriculus, brain, and testis. All the tissues collected were snap-frozen and stored in liquid nitrogen until RNA extraction.
Information regarding the abdominal adipose tissues of the 14th generation population of Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF; fat and lean line, each line 53 male broilers) had been published previously [17, 18], and they were kindly gift from professor Hui Li of Northeast Agricultural University. Briefly, after 14 generations of divergent selection for abdominal fatness, the abdominal fat percentage at 7 weeks of age in the fat broiler line was 4.45 times that of the lean line. The experimental groups were not randomized, but were divided into fat and lean groups based on the 14 generations of divergent selection for abdominal fat content. In addition, a sample size of 53 in each group achieved a confidence level of 91% to detect the difference in C/EBPZ expression between fat and lean birds. The experimenters were not blinded to groups on the test days, however, most of the subsequent analyses were performed in blinded conditions.
Vector construction
To clone the coding sequence (CDS) of chicken C/EBPZ, total RNA from abdominal fat tissue of 7-week-old AA broilers was reverse-transcribed using oligo(dT)-anchor primer and ImProm-II reverse transcriptase (Promega, Madison, USA). A set of primers, C/EBPZ -F1 (5′- GCGTCGACCATGGCGGCGCTCGGGGAGT-3′) and C/EBPZ -R1 (5′- GAAGATCTTCATCTTTTTGATTTCTTGCCTC-3′), was designed according to the chicken C/EBPZ sequences (GenBank accession NM_001031060.1). Additionally, LA Taq (TaKaRa, Dalian, China) was used to perform PCR reaction. After gel extraction, the PCR product of C/EBPZ was cloned into the pMD-18T vector (TaKaRa) for sequencing (BGI, Beijing, China). To generate the C/EBPZ overexpression vector (pCMV-HA-C/EBPZ), the C/EBPZ cDNA fragment, produced by digestion of pMD-18T-C/EBPZ using SalI and BglII (TaKaRa), was subcloned into the pCMV-HA vector (Clontech, Mountain View, USA).
The promoter-reporter plasmids of chicken PPARγ, C/EBPα, fatty acid synthase (FASN), adipocyte fatty acid-binding protein (FABP4) and Lipoprotein lipase (LPL) were described in literatures [19]. The promoter-reporter plasmid of chicken GATA binding protein 2 (GATA2), pGL4.10-GATA2 (–2 001/–1), is a luciferase reporter plasmid of pGL4.10 (Promega) containing the chicken GATA2 promoter (nucleotides –2001 bp to –1 bp, relative to the start site of NM_001003797.1), and the promoter-reporter plasmid of chicken GATA binding protein 3 (GATA3), pGL4.10-GATA3 (–296/+186), is the luciferase reporter plasmid of pGL4.10 (Promega) containing the chicken GATA3 promoter (nucleotides –296 bp to +186 bp, relative to the start site of X66844.1). Both of them were constructed by full-length sequence synthesis of BGI company.
Cell culture
Chicken stromal-vascular cells were isolated from 12-day-old chickens according to the procedure previously described [19], and the separated chicken stromal-vascular cells were considered as chicken preadipocytes and grown in DMEM/F12 medium supplemented with 10% fetal bovine serum and maintained at 37 °C in a humidified, 5% CO2 atmosphere. Before function study, all the cells had been treated with TransSafeTM Mycoplasma Elimination Reagent (TransMyco Plus) Kit (TransGen, Beijing, China) to remove mycoplasma contamination. The experimental groups of cells were randomized before transfection or induction by oleate, and the experimenters were blinded to groups on the test days.
RNA isolation and real-time reverse transcription-PCR
Total RNA was extracted from stored tissues (100 mg) or chicken preadipocytes using Trizol (Invitrogen, Carlsbad, USA) following the manufacturer’s protocol. RNA quality was assessed by denaturing formaldehyde agarose gel electrophoresis. Reverse transcription (RT) was performed using 1 μg of total RNA, an oligo(dT) anchor primer (Promega), and ImProm-II reverse transcriptase (Promega), and reverse transcription conditions were 25 °C for 5 min, 42 °C for 60 min, and 70 °C for 15 min.
Real-time PCR was used to detect gene expression using the SYBR Premix Ex Taq (Takara) on a 7500 Real-Time PCR System (Applied Biosystems, Foster City, USA) with the primers shown in Table 1. Chicken glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or beta-actin (ACTB) were used as internal references. Part (1 μL) of each RT reaction product was amplified in a 20-μL PCR reaction system. Reaction mixtures were incubated in an ABI Prism 7500 sequence detection system (Applied Biosystems), programmed to conduct 1 cycle at 95 °C for 30 s and 40 cycles at 95 °C for 5 s and 60 °C for 34 s. Moreover, dissociation curves were analyzed using Dissociation Curve 1.0 software (Applied Biosystems) for each PCR reaction to detect and eliminate possible primer-dimer artifacts. All reactions were performed in triplicate. The relative amounts of C/EBPZ transcripts were calculated using the comparative cycle-time method.
Western blot assays
Chicken preadipocytes transfected with pCMV-HA-C/EBPZ or pCMV-HA for 2 days were lysed in RIPA buffer (PBS, pH 7.4, containing 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and a protease inhibitor cocktail). Then, the cell lysates were added into 5× denaturing loading buffer and boiled in water for 5 min. Cell lysates were separated on 5–12% SDS-polyacrylamide gels and transferred to polyvinylidenedifluoride membranes. After incubation with the primary antibody for HA-tag [HA-tag (C29F4) rabbit mAB #3742, CST, Danvers, USA; 1:1 000], chicken ACTB (anti-β actin mAB #TA-09, ZSGB-BIO, Beijing, China;1:1 000) or chicken PPARγ [PPARγ (81B8) Rabbit mAb #2443, CST;1:1 000], a secondary horseradish peroxide-conjugated antibody (1:5 000; Beyotime, Beijing, China) was added, and a BeyoECL Plus kit (Beyotime) was used for detection.
RNA-seq analysis
The chicken preadipocytes transfected with pCMV-HA-C/EBPZ and empty vector (pCMV-HA) for 48 h, respectively, were collected using Trizol (Invitrogen) for RNA-seq analysis. The RNA-seq analysis and following data analysis were performed by Novogene company (Beijing, China).
Induction of chicken preadipocyte differentiation
Chicken preadipocytes were passaged and seeded into 6-well plates at a density of 1 × 105 cells/cm2. After 12 h, when the cells were 60–80% confluent, they were transfected with pCMV-HA-C/EBPZ or pCMV-HA using the FuGENE HD transfection reagent (Promega) according to the manufacturer’s recommendations. At 24 h after transfection, 160 μM oleate (Sigma) was added into the medium to induce preadipocyte differentiation for 48 h.
Oil red O staining
We followed the methods of Li et al. in 2014 [18]. Oil red O staining of intracellular lipid droplets was performed in 6-well plates. Chicken preadipocytes induced to differentiate for 48 h were washed with PBS and fixed in 10% formaldehyde for 10 min. After rinsing with distilled water, they were stained with 0.5% oil red O working solution, prepared by vigorously mixing three parts of a stock solution (0.5% oil red O in isopropanol; Sigma) with two parts of water for 5 min and filtering through a 0.4-µm filter. Excess staining was removed by rinsing twice with PBS. The dye was extracted by 1 mL isopropanol incubation, and quantitative assessment was obtained by spectrophotometric analysis of absorbance of the extracted dye at 500 nm.
Cell proliferation assay
After the chicken preadipocytes were transfected with pCMV-HA-C/EBPZ and pCMV-HA, respectively, and incubation for 24 h, these cells were passaged and seeded into 96-well plates at a concentration of 5000 cells per well, respectively. At the designated time points after passage, including 24 h, 48 h, 72 h, 96 h and 120 h, the cell viability was detected using CellTiter-Glo® Luminescent Cell Viability Assay (Promega) following the manufacturer’s protocol.
Flow cytometric analysis
Chicken preadipocytes were seeded in 6-well plates. Transfection was performed when the cells were about 60% confluent. After transfection for 48 h, cells were digested by trypsin, and then ceased the digestion using DMEM/F12 with 10% FBS. The cells were transferred to 15 mL micro-centrifuge tubes and centrifuged under the condition of 1000 rpm for 5 min at 4 °C to discard the supernatant. The remained cells were re-suspended and washed by 1 mL cold PBS. Then PBS was discard after centrifugation of 1000 rpm for 5 min at 4 °C, and the cells were fixed in 100% ethanol overnight at −20 °C. After that, the fixed cells were stained with Cell cycle staining Kit (MultiSciences, Hangzhou, China), and the flow cytometric analysis was performed using BD FACSAriaTM III flow cytometer (BD Biosciences, San Jose, USA), and the data were analyzed using FlowJo software.
Luciferase reporter assays
The luciferase reporter assays were performed in chicken preadipocytes in 12-well dishes. In transfection assays, a fixed amount of total DNA (1 μg) was transfected in each well using FuGENE HD transfection reagent (Promega). The transfection system for plasmids was shown in Table 2. After transfection and incubation for 48 h, the cells were lysed in 250 μL of 1-time passive lysis buffer (Promega), and portions of the lysate were subjected to assays for firefly and renilla luciferase using the Dual-Luciferase Reporter Assay System (Promega). Promoter activity of each construct was expressed as the ratio of Firefly / Renilla luciferase activity.
Sequence analysis
The sequences used were all from NCBI protein database. Because the predicted sequences might be updated frequently, only the sequences of C/EBPZ officially certified by NCBI and titled by ‘NP_’ were used for sequence analysis. The sequences used included six species, including human, mouse, rat, chicken, frog and zebrafish (Table 3). DNAMAN was employed to get the overall identity of the multiple sequence alignment, and Clustal Omega was used to get the percent identity matrix. The Genomicus software program (http://www.dyogen.ens.fr/genomicus-62.02/cgi-bin/search.pl) was used to identify syntenic genes near C/EBPZ in the genomes of animals.
Statistical analysis
All statistical analyses were performed using the R software system (version R 3.5.1; https://cran.rproject.org/bin/windows/base/old/3.5.1/). Sample size estimation was performed using the R package ‘pwr’. The Shapiro–Wilk test was used to test the normality of data. The difference analysis between two groups was performed by two tailed Student’s t test or Wilcoxon rank sum test. The difference analysis in more than two groups was analyzed by ANOVA or Kruskal–Wallis tests, and comparisons among more than two groups were performed by Duncan’s multiple tests. The data that conformed to the normal distribution were directly applied to Duncan’s multiple tests, and the data that did not conform to the normal distribution were sorted rank firstly, and the ranks were then applied to Duncan’s multiple tests.
The chicken C/EBPZ transcripts of abdominal fat tissues from NEAUHLF were analyzed by Scherier–Ray–Hare tests with the following model:
Where Y is the dependent variable (the relative C/EBPZ expression), μ is the population mean, A is the fixed effect of the age, L is the line (broiler line selected by high and low abdominal fat content) as fixed effect, A*L is the interaction of age and line, and e is the random error.
The expression data of C/EBPZ of human adipose tissues (Supplementary Table 1) were achieved from the human protein atlas (https://www.proteinatlas.org/) [20] and analyzed by Cochran–Mantel–Haenszel test. The relationship between the pTMP of C/EBPZ and the percentage of adipocytes in adipose tissue was analyzed by Kendall rank test. The relationships between the pTMPs of C/EBPZ and other genes in adipose tissue were analyzed by Spearman tests using GEPIA online serves.
The data were presented as mean ± standard deviations (SD) unless otherwise indicated, and differences were considered significant at P < 0.05 unless otherwise indicated.
Results
Bioinformatics analysis of C/EBPZ expression in human adipose tissue
The data from human protein atlas showed that there was a low expression level of C/EBPZ in adipocytes (Fig. 1A), and the pTPM of C/EBPZ in adipose tissue was negative related to the percentage of adipocytes (τb = −1, P = 0.0415; Fig. 1B).
The pTPM data of C/EBPZ did not conform to a normal distribution (Shapiro–Wilk test; W = 0.99531, P = 0.0156). Cochran–Mantel–Haenszel test showed that C/EBPZ transcripts of human adipose tissue were significantly associated with the location (H = 6.4901, P = 0.0108) and age (H = 18.2767, P = 0.0026), and there was no significant association of them with sex (H = 0.4001, P = 0.5270).
Wilcoxon rank sum test showed that the C/EBPZ transcripts of subcutaneous adipose tissue were significantly greater than those of visceral adipose tissue (W = 87646, P = 0.004438; Fig. 1D). Duncan’s multiple tests showed that the C/EBPZ expression increased with age (P < 0.05; Fig. 1E). In addition, the C/EBPZ transcripts of subcutaneous adipose tissue were significantly greater than those of visceral adipose tissue at the ages of 20–29 years and 30–39 years (P < 0.05; Fig. 1F).
Additionally, Spearman correlation tests showed that the C/EBPZ transcripts of adipose tissue were negatively associated with those of PPARγ, C/EBPα, FASN, LPL, Perilipin 1 (PLIN1) and Sterol regulatory element binding transcription factor 1 (SREBP1) (r < 0, P < 0.05), and they were positively associated with the transcripts of KLF7 and DDIT3 (r > 0, P < 0.05, Supplementary Fig. 1).
Sequence and tissue expression pattern of chicken C/EBPZ
Chicken C/EBPZ was first reported in a study to sequence and characterize a large number of full-length cDNAs from bursal lymphocytes [21]. However, to date, there was no further report on chicken C/EBPZ. In this study, sequence analysis showed that there were 1076 amino acids in predicted chicken C/EBPZ, and it could express a protein about 123 kD. Chicken C/EBPZ has a high similarity in protein sequence to its human and murine orthologs (>60%, amino acids identity, Table 4). Especially, the conserved domains related to the interaction of NF-Y trimer [8] and function of proribosome [22] also could be found in chicken C/EBPZ (Supplementary Fig. 2). In addition, C/EBPZ was in a conserved chromosome region during the evolution of animals, and the linkage group of CDC42EP3-QPCT-PRKD3-C20RF56-C/EBPZ could be detected in both human and chicken chromosomes (Supplementary Table 2).
Real-time RT-PCR analysis showed that C/EBPZ was expressed ubiquitously in various chicken tissues at the age of 7 weeks, including duodenum, abdominal fat, brain, pectoralis, proventriculus, heart, ileum, kidney, jejunum, liver, leg muscle, gizzard, pancreas, spleen, and testis (Supplementary Fig. 3). The data of C/EBPZ transcripts did not conform to a normal distribution (Shapiro–Wilk test; W = 0.647974, P < 0.0001). Kruskal–Wallis analysis showed that the C/EBPZ transcripts were significantly associated with the category of tissues (H = 70.1, P < 0.001). Duncan’s multiple tests showed that among of them, pancreas, jejunum, testis and abdominal fat had high expression levels of C/EBPZ, and leg muscle, liver, pectoralis, heart and brain had low expression levels of C/EBPZ (P < 0.01, Supplementary Fig. 3).
Expression pattern of C/EBPZ in visceral adipose tissue during chicken development
The expression pattern of C/EBPZ in the abdominal fat tissues of 1- to 12-week-old broilers from NEAUHLF was studied by real-time PCR. The results showed that C/EBPZ was expressed in all the abdominal fat tissues tested. The relative C/EBPZ expression data (C/EBPZ /ACTB) did not conform to a normal distribution (Shapiro–Wilk test; W = 0.92498, P < 0.0001). Scherier–Ray–Hare test showed that C/EBPZ transcripts significantly associated with age (H = 33.560, P = 0.00043) and broiler line (selected by high and low abdominal fat content, respectively; H = 8.949, P = 0.00278), and there was no significant association of C/EBPZ transcripts with the interaction of age and line (H = 10.822, P = 0.45826).
Wilcoxon rank sum test showed that the C/EBPZ expression of adipose tissue was significantly greater in lean broilers than in fat broilers (W = 927, P = 0.002578; Fig. 2A). Duncan’s multiple tests showed that the C/EBPZ expression decreased during development, and it peaked at 1 week of age and reached its nadir at 12 weeks of age (P < 0.01; Fig. 2B).
Additionally, at the ages of 7 and 9 weeks, the C/EBPZ expression was significantly greater in lean males than in fats (P < 0.05; Fig. 2C). No significant difference was detected at the other ages (P > 0.05, Fig. 2C).
RNA-seq analysis of chicken preadipocytes with C/EBPZ overexpression
To investigate the role of C/EBPZ in chicken adipose tissue, C/EBPZ was overexpressed in chicken preadipocytes by transfection with the plasmid of pCMV-HA-C/EBPZ for 48 h. Western blot analysis showed that chicken preadipocytes transfected with pCMV-HA-C/EBPZ for 48 h could express a HA-tag protein around 130 kD, and no similar protein was detected at this level in the cells transfected with plasmid of pCMV-HA (empty vector, EV; Fig. 3A).
RNA-seq was employed to study the effect of C/EBPZ overexpression on mRNA transcriptome of chicken preadipocytes (Fig. 3B), and the reads per kilobase of exon model per million mapped reads (FPKM) were got for expression analysis (Fig. 3C).
Totally, the valid data of 17927 genes were obtained, and negative binomial distribution test showed that there were 448 genes upregulated and 354 genes downregulated in the C/EBPZ-overexpressed preadipocytes compared with EV group (P < 0.05; Fig. 3D and Supplementary Table 3), and the FPKM of C/EBPZ was significantly greater in C/EBPZ-overexpressed cells than in EV group (P < 0.05; Fig. 3E).
Real-time PCR was employed to verify the reliability of the results of RNA-seq. The results showed that all the four genes tested were differentially expressed between EV and C/EBPZ group (Fig. 3F), consistent with the result of RNA-seq.
Gene Ontology (GO) enrichment analysis showed that the differential expression genes were enriched in 8 of GO terms at level of adjusted P value < 0.05 (Supplementary Table 4), namely, regulation of cellular ketone metabolic process (GO:0010565), negative regulation of protein serine/threonine kinase activity (GO:0071901), negative regulation of protein modification process (GO:0031400), negative regulation of MAPK cascade (GO:0043409), modulation by virus of host morphology or physiology (GO:0019048), modification by symbiont of host morphology or physiology(GO:0044003), negative regulation of MAP kinase activity (GO:0043407), and inactivation of MAPK activity (GO:0000188, Fig. 3G).
Additionally, Gene Set Enrichment Analysis (GSEA) showed that 729 and 99 gene sets of GO terms were significantly enriched in C/EBPZ-overexpressed cells and EV group, respectively (P < 0.01, Supplementary Tables 5–6). Among them, the gene set related to the adipose tissue development (GO:0060612) was significantly upregulated in C/EBPZ-overexpressed cells, and the gene set related to RNA polymerase II basal transcription factor binding (GO:0001091) was significantly upregulated in EV group (Fig. 3I). However, none of them was significantly enriched at FDR < 25%.
The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that C/EBPZ was involved in the DNA replication (GGA03030), Glycosaminoglycan biosynthesis (GGA00534/GGA00532), Autophagy (GGA04136), and Arginine and proline metabolism (GGA00330) (P < 0.05, Fig. 3H and Supplementary Table 7). However, none of them reach the level of adjusted P value < 0.05.
Additionally, GSEA analysis showed that 16 and 13 gene sets of KEGG pathways were significantly enriched in the C/EBPZ-overexpressed cells and EV group, respectively (P < 0.01, Supplementary Tables 8–9), and two gene sets were significant enriched at FDR < 25%. They were ERBB signaling pathway (GGA04012) which upregulated in C/EBPZ-overexpressed cells and fatty acid degradation (GGA00071) which upregulated in EV group, respectively (Fig. 3J).
The expression of C/EBPZ in chicken preadipocytes during differentiation
The expression pattern of C/EBPZ during the differentiation of chicken preadipocytes induced by oleate was studied using real-time PCR. The result showed that C/EBPZ was expressed in chicken preadipocytes at every time point detected. Shapiro–Wilk tests showed that the data conform to a normal distribution (P > 0.05). Student’s t tests showed that there was no significant difference of C/EBPZ expression at any time point between negative control (NC) and preadipocytes induced by oleate (P > 0.05; Fig. 4A). Using the average expression level of C/EBPZ of NC at each time point to normalize the expression of C/EBPZ in preadipocytes induced by oleate, and Duncan’s multiple tests showed that the normalized expression of C/EBPZ was significant decreased with the time of oleate induced, especially the C/EBPZ expression at 24 h after oleate-induced was significantly greater than other time points (P < 0.05; Fig. 4B).
Effect of C/EBPZ overexpression on chicken adipogenesis in vitro
To further reveal the function of chicken C/EBPZ in adipogenesis, the effect of C/EBPZ overexpression on the proliferation and differentiation of chicken preadipocytes was studied in vitro. The result showed that C/EBPZ-overexpressing cells (Fig. 4G) exhibited an enhanced ability in cell proliferation at 96 h and 120 h after transfection compared to EV-transfected preadipocytes (P < 0.05, Fig. 4C). In addition, the flow cytometric analysis showed that after the transfection of pCMV-HA-C/EBPZ for 48 h, the proportion of G1 phase cells in C/EBPZ-overexpressed preadipocytes was significantly lower than that of EV group (P < 0.05), while there was no significant difference in S or G2 phase between them (Fig. 4D). Additionally, compared with EV-transfected chicken preadipocytes, C/EBPZ-overexpressing chicken preadipocytes exhibited a decrease in the intracellular lipid accumulation as evidenced by oil red O staining (Fig. 4E) and a quantitative assessment (P < 0.05, Fig. 4F).
Effect of C/EBPZ overexpression on the promoter activities of function genes of chicken adipose tissue
The effect of C/EBPZ overexpression on the promoter activities of several function genes of adipose tissue were studied using luciferase reporter assay. The results showed that the C/EBPZ overexpression significantly suppressed the promoter activities of PPARγ, C/EBPα, FASN, and LPL in chicken preadipocytes (P < 0.05, Fig. 5), and no significant effect on the promoter activity of GATA3 was found (P > 0.05, Fig. 5). In addition, C/EBPZ overexpression significantly facilitated the promoter activities of chicken GATA2 and FABP4 in chicken preadipocytes (P < 0.05, Fig. 5). Additionally, RT-PCR and western blot analysis showed that overexpression of C/EBPZ significantly inhibited PPARγ expression in chicken preadipocytes (P < 0.05, Fig. 6).
Discussion
Expression of C/EBPZ in human adipose tissue
The major functions of adipose tissue are to store and mobilize fat, and it plays important roles in the insulin resistance pathogenesis and development of T2DM [3]. C/EBPZ (Gene ID: 10153) is an interesting gene with a complex background of nomenclature [5], and its function in adipose tissue is not clear now.
Herein, to find out whether C/EBPZ plays a role in adipose tissue, the expression pattern of C/EBPZ in human adipose tissue was studied using bioinformatics methods. The results showed that the C/EBPZ transcripts of human adipose tissue were negatively related to the percentage of adipocytes (Fig. 1), and they were negatively and positively associated with the transcripts of positive and negative regulators of adipogenesis, respectively (Supplementary Fig. 1), suggested that C/EBPZ might play a negative role in adipogenesis.
The ectopic fat hypothesis suggests that visceral adipose tissue confers more increased metabolic risk than subcutaneous adipose tissue [23]. The C/EBPZ transcripts were greater and more stable in subcutaneous adipose tissue than in visceral adipose tissue (Fig. 1), suggested that C/EBPZ might be involved in different contribution of adipose tissue to metabolic risk. In addition, age changes in the metabolism of the adipose tissue and the part played by fatty tissue in decreasing the serum free fatty acid response [24]. It is possible that the increasing of C/EBPZ expression of adipose tissue with age (Fig. 1), might be result from the metabolism change of adipose tissue, and C/EBPZ might be involved in the metabolic regulation of adipose tissue.
Function of C/EBPZ in chicken adipose tissue
C/EBPZ was conserved in protein sequence and synteny pattern of chromosome between human and chicken, and both the C/EBPZ transcripts of human [20] and chicken (Supplementary Fig. 3) were ubiquitously expressed in various tissues, suggested that chicken and human C/EBPZs might have similar functions.
Chicken had been reported as an ideal animal model for obesity formation and metabolic disease [25]. In the current study, the results showed that C/EBPZ transcripts of visceral adipose tissue were greater in lean broilers than in fat broilers and decreased during the growth of adipose tissue (Fig. 2), suggested that chicken C/EBPZ might be a negative regulator of visceral fat development, consistent with the finding in human.
The development of adipose tissue involves hyperplastic (proliferation) and hypertrophie growth (differentiation) of adipocytes, and histological analysis report showed that the difference in visceral adipose tissue between fat and lean birds at the age of 7 weeks was resulted from the different size and number of adipocytes [26]. The C/EBPZ expression of visceral adipose tissue was greater in lean males than in fat males at 7 weeks of ages (Fig. 2), it is possible that C/EBPZ regulated the differentiation or proliferation of adipocytes in vivo.
GSEA analysis of RNA-seq showed genes related to adipose tissue development (GO:0060612) were upregulated in C/EBPZ-overexpressed chicken preadipocytes (Fig. 3), providing another evidence that C/EBPZ is a regulator of adipose tissue development. However, this was not consistent with the negative role of C/EBPZ in adipogenesis. It is possible that multiple roles of C/EBPZ exited in adipose tissue. In addition, most adipocytes had been developed in chicken visceral adipose tissue at 7 weeks of age [26], C/EBPZ expression could be detected at all the tested ages from 1 to 12 weeks in chicken visceral adipose tissue and differentially expressed at 9 weeks of age between fat and lean birds, suggested that C/EBPZ might also play a role in developed adipose tissue.
Additionally, GSEA analysis of RNA-seq showed that genes associated with RNA polymerase II basal transcription factor binding (GO:0001091) were downregulated in C/EBPZ-overexpressed preadipocytes (Fig. 3), suggested that C/EBPZ could function as a transcription factor in chicken adipose tissue, consistent with the previous reports in human [7, 8].
The function of C/EBPZ in chicken adipogenesis was further studied in vitro, and the results showed that C/EBPZ expression was decreased during differentiation of chicken preadipocytes (Fig. 4), which was similar as many other negative regulators of adipogenesis [3], for example, KLF2 [18] and KLF7 [19]. In addition, red O staining analysis showed that C/EBPZ overexpression inhibited the differentiation of chicken preadipocytes into adipocytes-like cells (Fig. 4). These results indicated that C/EBPZ was a negative regulator of chicken adipogenesis in vitro, too.
The adipogenesis is regulated by an elaborate network of transcription factors, and PPARγ oversee the entire terminal differentiation process at the center of this network [3]. In addition, C/EBPα could facilitated PPARγ expression and adipogeneis [3], and GATA2 and FABP4 could attenuate adipogenesis through the suppression of PPARγ expression [27, 28]. Luciferase reporter assays showed that C/EBPZ overexpression suppressed the promoter activities of chicken PPARγ and C/EBPα, and promoted the promoter activities of chicken GATA2 and FABP4 (Fig. 5), further indicated that C/EBPZ was a negative regulator of adipogenesis. In addition, western blot analysis showed that C/EBPZ overexpression inhibited PPARγ expression (Fig. 6). Thus, mechanistically, C/EBPZ might inhibit chicken adipogenesis at least through the suppression of PPARγ expression directly or indirectly (Fig. 6D).
LPL and FASN plays important roles in the transport of lipids [29] and de novo lipogenesis in adipose tissue [30], respectively. Overexpression of C/EBPZ inhibited the promoter activities of chicken FASN and LPL (Fig. 5), which was in line with the negative role of C/EBPZ in chicken adipose tissue development, suggested that C/EBPZ might also regulate fat accumulation of adipocytes (Fig. 6D). In addition, FABP4 acts as a fatty acids chaperone that regulates the basal and hormone-stimulated adipocyte fatty acid efflux [31]. Overexpression of C/EBPZ promotes the promoter activity of chicken FABP4 (Fig. 5), and the genes associated with fatty acid degradation (GGA00071) downregulated in C/EBPZ-overexpressed cells (Fig. 3), shed a light on that C/EBPZ might play a role in the control of fatty acid efflux in adipocytes (Fig. 6D). Together, chicken C/EBPZ might also regulate the function of mature adipocytes in vitro.
The proliferation of preadipocytes is another important process of adipose tissue development [32]. KEGG analysis of RNA-seq showed that genes involved in DNA replication (GGA03030) were differentially expressed between C/EBPZ-overexpressed preadipocytes and EV group (Fig. 3), suggested that chicken C/EBPZ could regulate preadipocyte proliferation.
C/EBPα is also a cell cycle regulator that promotes anti-proliferation through inhibition of cyclin-dependent kinases 2, 4, and 6 and repression of S-phase gene transcription [33]. In the current study, C/EBPZ-overexpressed preadipocytes showed an increased proliferation ability (Fig. 4), and chicken C/EBPZ overexpression suppressed the promoter activities of C/EBPα (Fig. 5), indicated that C/EBPZ could promote the proliferation of chicken preadipocytes (Fig. 6D). In addition, the flow cytometric analysis showed that the proportion of G1 phase cells in C/EBPZ-overexpressed preadipocytes was significantly lower than that of EV group, demonstrated that C/EBPZ might improve preadipoyctes proliferation at least by inhibiting C/EBPα expression and inducing the transition of cell cycle from G1 phase to S-phase.
GO analysis of RNA-seq showed that genes involved in the pathological process of infectious diseases (GO:0019048/ GO:0044003) were differentially expressed between C/EBPZ-overexpressed cells and EV group (Fig. 3G), which was consistent with the previous report that C/EBPZ took a part in the viral-human protein-protein interaction for SARS-CoV-1, MERS-CoV, and SARS-CoV-2 [15]. In addition, the present study showed that C/EBPZ overexpression regulated adipogenesis, these shed a light on that further study on the biological function of C/EBPZ in adipose tissue might be of signification for revealing the molecular mechanism underlying the connection of metabolic diseases and infectious diseases, thereby to improve the prognosis of infectious diseases with metabolic diseases.
In conclusion, the current study provided novel insights into the mechanisms underlying development and function of adipose tissue and demonstrated that C/EBPZ might be a new regulator of the development and function of adipose tissue. The results of this study shed a light on revealing the molecular mechanism of adipogenesis and metabolic diseases.
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Acknowledgements
This work was supported by the grant from the National Natural Science Foundation of China (Nos. 31960647 and 31501947), the Youth Innovative Talents Project of Shihezi University (No. CXBJ201905) and the Program for High-level personnel start at Shihezi University (No. RCZX201552). We thank Professor H. Li for the generous offer of abdominal adipose tissues of the 14th generation population of NEAUHLF.
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ZZ conceived the project and designed experiments. ZZ, YC and XP performed bioinformatics analysis and data analysis. YC, LG, TL, QG and JC performed the molecule or cell biology experiments. YZ, XW and ZL constructed and prepared plasmids. ZZ and YC wrote the manuscript.
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Chen, Y., Gao, L., Lin, T. et al. C/EBPZ modulates the differentiation and proliferation of preadipocytes. Int J Obes 46, 523–534 (2022). https://doi.org/10.1038/s41366-021-01020-z
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DOI: https://doi.org/10.1038/s41366-021-01020-z