Abstract 12

The prediction studies of type I diabetes in the preclinical period were initially based on the detection of islet cell antibodies (ICA). Others antibodies including those to glutamic acid decarboxilase (A-gad), insuline autoantibody (IAA) have been added to improve the predictive capacity and more recently the autoantibodies to tirosine phosphatase ICA 512. To analyse their potencial for screening children, the aim of this study was to determine the frequency of autoantibodies to ICA 512 in recent-onset IDDM and to compare this with previously ascertained frequency for other islet cell autoantibodies: A-GAD; IAA and ICA. We studied 60 patients with IDDM (48,4% girls and 51,6% boys; mean age: 10,22 ± 3,8a) and 60 sex and age controls without personal or family history of autoimmune disease. The assay were performed using fasted serum samples, for the diabetic group they were taken within 72 hours of the start of insuline therapy. ICA 512 As and A-GAD were tested by immunoprecipitation; IAA by radiobanding assay an ICA by indirect immunofluorescence. Results: Table

Table 1 No caption available.
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Conclusions: Our results indicate that positivity in a combination of test is more valuable for prediction of IDDM than a result for any single autoantibody and that the combination of ICA 512 and A-GAD has a sensitivity equivalent to ICA; IAA and A-GAD