ALLOPURINOL (ALLO), DEFEROXAMINE (DFO), AND BRAIN ENERGY METABOLISM OF NEWBORN PIGLETS FOLLOWING HYPOXIA-ISCHEMIA

Abstract 124

In the present study we tested the hypothesis that ALLO or DFO preserved brain energy metabolism in newborn piglets following hypoxia-ischemia (HI). Methods: Following instrumentation, HI was induced by occluding both carotid arteries and decreasing FiO₂. After 1 hour of HI, the brain was reperfused for an additional 24 hours. Using ¹H- and ³¹P-Magnetic Resonance Spectroscopy for measuring cerebral energy metabolism, phosphocreatine/inorganic phosphate (PCr/Pi), and lactate/N-acetylaspartate (Lac/NAA) were measured. Immediately upon reperfusion, piglets received either ALLO (20mg/kg iv, n=4), DFO (10mg/kg iv, n=4), or normal saline (PLAC, n=4). Results: All results are shown as mean (SD) for all treatments from normoxia (t=0) until 24 h post-HI. PCr/Pi ratios are shown as % of baseline values. (Table) Differences between the groups were not significant. Conclusions: ALLO tended to prevent a fall in PCr/Pi at 3 and 24 h post-HI. Both ALLO and DFO tended to prevent an increase in Lac/NAA at 24 h post-HI. Further studies are necessary to confirm these observations.

Table 1 No caption available.