Abstract
The cause of growth velocity decrease in children affected by chronic inllammatory diseases is unknown. The response to infection involves production of Interleukin-1β(IL-1), from monocyte cells. The study is aimed at examining the effect of IL-1 on acute release of somatostatin(SS) from rat hypothalamus, and the intracellular mechanism involved. Hypothalami from male Wistar rats were incubated with IL-1 and medium assayed for SS by RIA. IL-1 (1-10 U/ml) increased the release of SS (Tab. 1)
SS is expressed as pg/hypothalamus/20 min.; *= p<0.01, **=p<0.001 The production of SS by IL-1 was inhibited by the cyclo-oxygenase inhibitor, naproxen (NAP) (Tab.2) and by the cyclo-lipo-oxygenase inhibitor BW775 (Tab.3), but not by the lipo-oxygenase inhibitor BWA4C (Tab.4). The inhibitors are expressed in μg/ml. IL-1 is a potent stimulator of SS; the intracellular mechanism involved in this action is via cyclo-oxygenase paithway. This action of IL-1 suggest an explanation for the paradoxical GH-responses to TRH in some short children with chronic diseases.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Spagnoli, A., Navarra, P., Tsagarakis, S. et al. 158 IN VITRO ACURATE RELEASE OF SOMATOSTAIN FROM RAT HYPOTHALAMUS: EFFECT OF IL-1. Pediatr Res 30, 654 (1991). https://doi.org/10.1203/00006450-199112000-00188
Issue Date:
DOI: https://doi.org/10.1203/00006450-199112000-00188