Abstract
The effects of intravenous (IV) endotoxin (LPS) (E.coli 0111B4) on neutrophil (PMN) function in vitro and in vivo in an experimental pig model was examined.Six wk. old pigs were anesthetized with halothane, intubated and ventilated. Intrapleural catheters (10Fr) were inserted. LPS (1.5 ug/kg I.V.) was given and 30 mins. later 5ml zymosan activated pig serum (ZAS) or normal saline as control were placed into the pleural space. Leukocyte migration in vivo was monitored by hourly pleural lavages. In non-LPS challenged pigs,PMN migration (×106)began by lhr (40±10) and peaked at 4hr (220±50).Immature PMN (bands) were observed at 2hr(7±0.8) and peaked by 4hr (10±3). Monocytes migrated by lhr (5±1) and peaked at 6hr (35±5). Following LPS, blood PMN and bands fell by 50%, after which the number of bands increased approximately 10 fold. Migration of PMN into the pleural space was similar in LPS and non-LPS treated pigs. However, in LPS treated pigs, the number of bands migrating was significantly greater and mono - cytes significantly less. In vitro, migration and bacterial killing by blood PMN was transiently reduced after LPS. Furthermore, the bactericidal capacity of pleural PMN was significantly greater than blood PMN in both LPS and non-LPS treated pigs. Our findings illustrate the importance of assessing PMN function both in vivo and in vitro when studying the biologic effects of LPS. (MRC Canada)
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Biggar, W., Barker, C., Crawford, L. et al. BLOOD AND INFLAMMATORY NEUTROPHIL FUNCTION FOLLOWING EXPERIMENTAL ENDOTOXEMIA. Pediatr Res 18 (Suppl 4), 269 (1984). https://doi.org/10.1203/00006450-198404001-01058
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DOI: https://doi.org/10.1203/00006450-198404001-01058