Abstract
Thermolysin [EC 3.4.24.4] was immobilized by use of a carbamoyl sulfonated urethane prepolymer. The pH dependence of apparent activity of the immobilized thermolysin was dependent on the species of the introduced diamine; compared with the one obtained with hexamethylenediamine, that prepared with l-lysine showed an optimum pH shifted to the basic side while an addition of triethylenetetramine shifted the pH profile to the acidic side. The activity after repeated use was improved by treatment with glutaraldehyde and also by an addition of cobalt(II) ion. The metal ion at the active site of the immobilized enzyme was effectively (though not completely) replaced by a treatment with ethylenediaminetetraacetic acid and the successive addition of metal ion.
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Kunugi, S., Morikawa, Y., Ishida, M. et al. Immobilization of Thermolysin by Water-Soluble Reactive Polyurethane: Influence of Charges on the Matrix and Metal-Exchange at the Active-Site. Polym J 19, 269–273 (1987). https://doi.org/10.1295/polymj.19.269
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DOI: https://doi.org/10.1295/polymj.19.269