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Reply to “Mechanisms of APOBEC3G-catalyzed processive deamination of deoxycytidine on single-stranded DNA”

Nature Structural & Molecular Biology volume 16, pages 455–456 (2009)Cite this article

Nowarski et al. reply:

In their correspondence, Chelico and colleagues define a processive enzyme as one which “catalyzes multiple reactions on a single substrate before acting on a different substrate”. They have previously argued that apolipoprotein B mRNA–editing enzyme, catalytic polypeptide–like (APOBEC) 3G (A3G) processivity is based mostly on one-dimensional (linear) sliding on DNA and also involves microscopic jumping (hopping), that is, local dissociation and reassociation at a nearby locus on the DNA1. We agree with Chelico and colleagues that A3G can act processively on ssDNA. However, we differ on the conclusions regarding A3G's translocation mechanism and have reservations toward the use of the term 'processivity' to describe it. Instead, we propose that intersegmental transfer can explain the translocation mechanism and hypermutation properties of A3G.

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Authors and Affiliations

  1. Department of Pathology and the Lautenberg Center for General and Tumor Immunology, the Hebrew University, Hadassah Medical School, Jerusalem, Israel. e-mail: mkotler@cc.huji.ac.il,

    Roni Nowarski, Elena Britan-Rosich & Moshe Kotler

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  1. Roni Nowarski
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  2. Elena Britan-Rosich
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  3. Moshe Kotler
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Nowarski, R., Britan-Rosich, E. & Kotler, M. Reply to “Mechanisms of APOBEC3G-catalyzed processive deamination of deoxycytidine on single-stranded DNA”. Nat Struct Mol Biol 16, 455–456 (2009). https://doi.org/10.1038/nsmb0509-455

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