Abstract
The structure of the protein known both as neuronal nitric oxide synthase inhibitory protein, PIN (protein inhibitor of nNOS), and also as the 8 kDa dynein light chain (LC8) has been solved by X-ray diffraction. Two PIN/LC8 monomers related by a two-fold axis form a rectangular dimer. Two pairs of α-helices cover opposite faces, and each pair of helices packs against a β-sheet with five antiparallel β-strands. Each five-stranded β-sheet contains four strands from one monomer and a fifth strand from the other monomer. A 13-residue peptide from nNOS is bound to the dimer in a deep hydrophobic groove as a sixth antiparallel β-strand. The structure provides key insights into dimerization of and peptide binding by the multifunctional PIN/LC8 protein.
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References
Moncada, S. J. Hypertens. Suppl. 12, S35–39 (1994).
Yun, H.Y., Dawson, V.L. & Dawson, T.M. Crit. Rev. Neurobiol. 10, 291 –316 (1996).
Jaffrey, S.R. & Snyder, S.H. Science 274, 774–777 (1996).
Huang, E.P. Curr. Biol. 7, R350–352 (1997).
Greenwood, M.T., Guo, Y., Kumar, U., Beauséjours, S. & Hussain, S.N.A. Biochm. Biophys. Res. Commun. 238, 617–621 (1997).
Gillardon, F., Krep, H., Brinker, G., Lenz, C., Böttiger, B. & Hossmann, K.A. Neuroscience 84, 81–88 ( 1998).
Hirokawa, N. Science 279, 519–526 ( 1998).
King, S.M. & Patel-King, R.S. J. Biol. Chem. 270, 11445–11452 (1995).
King, S.M. et al. J. Biol. Chem. 271, 19358– 19366 (1996).
Benashski, S.E., Harrison, A., Patel-King, R.S. & King, S.M. J. Biol. Chem. 272, 20929–20935 (1997).
Pazour, G.J., Wilkerson, C.G. & Witman, G.B. J. Cell Biol. 141, 979– 992 (1998).
Dick, T., Ray, K., Salz, H.K. & Chia, W. Mol. Cell. Biol. 16, 1966–1977 ( 1996).
Phillis, R. Statton, D., Caruccio, P. & Murphey, R.K. Development 122, 2955–2963 (1996).
Espindola, F.S., Cheney, R.E., King, S.M., Suter, D.M. & Mooseker, M.S. Mol. Biol. Cell 7, 372A (1996).
Crépieux, P. et al. Mol. Cell. Biol. 17, 7375– 7385 (1997).
Tochio, H., Ohki, S., Zhang, Q., Li, M. & Zhang, M. Nature Struct. Biol. 5, 965–969 (1998).
Jaenicke, T. et al. Genomics 8, 194–206 (1990).
Paschal, B. M., Mikami, A., Pfister, K.K., de Vallee & R.G. J. Cell Biol. 118, 1133–1143 (1992).
Van Der Bliek, A. M. et al. J. Cell Biol. 122, 553– 563 (1993).
Rodriguez-Crespo, I. Straub, W., Gavilanes, F., & Montellano, P.R.O. Arch. Biochem. Biophys. 359, 297–304 (1998).
Collaborative Computational Project, Number 4. Acta Crystallogr. D50, 760– 763 (1991).
Terwilliger, T. C., Kim, S. H. & Eisenberg, D. Acta Crystallogr. A43, 1– 5 (1987).
Jones, T. A. et al. Acta Crystallogr. A47, 110– 119 (1991).
Kleywegt, G. J. & Jones, T. A. In From First Map to Final Model (eds. Bailey, S., Hubbard, P. & Waller, D.) 59–66 (SERC Daresbury Laboratory, Warrington; 1991).
Brunger, A.T. X-PLOR manual, Version 3.1. (Yale University Press, New Haven, Connecticut; 1992).
Kraulis, P.J. J. Appl. Crystallogr. 24, 946–947 (1991).
Wallace, A.C., Laskowski, R. & Thornton, J. Prot. Engng. 8, 127– 129 (1995).
Nicholls, A., Bharadwaj, R. & Honig, B. Biophys. J. 64, 166– 167 (1993).
ClustalW (http://www2.ebi.ac.uk/clustalw/).
Barton, G. J. Prot. Engng. 6, 37–40 ( 1993).
Acknowledgements
The authors thank M.A. Iwamoto and T.K. Ly for help with PIN/LC8 crystallizations and S. Ealick and S. Liu for helpful discussions. This work was partially supported by USPHS grants (J.C., S.H.S., and S.R.J.) and a Research Scientist Award (S.H.S.).
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Liang, J., Jaffrey, S., Guo, W. et al. Structure of the PIN/LC8 dimer with a bound peptide. Nat Struct Mol Biol 6, 735–740 (1999). https://doi.org/10.1038/11501
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DOI: https://doi.org/10.1038/11501
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