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NMR structure of a parallel homotrimeric coiled coil

Nature Structural Biology volume 5, pages 687–691 (1998)Cite this article

Abstract

The solution structure of the oligomerization domain of cartilage matrix protein (also known as matrilin-1) has been determined by heteronuclear NMR spectroscopy. The domain folds into a parallel, disulfide-linked, three-stranded, α-helical coiled coil, spanning five heptad repeats in the amino acid sequence. The sequence of the first two heptad repeats shows some deviations from the consensus of hydrophobic and hydrophilic residue preferences. While the corresponding region of the coiled coil has a higher intrinsic flexibility, backbone α-helix and superhelix parameters are consistent with a regular coiled coil structure.

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Figure 1: Interchain distance restraints.
Figure 2: Coiled-coil packing.
Figure 3: Core and surface of the coiled coil.

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Acknowledgements

We thank M.J.J. Blommers, W. Jahnke, and W. Stark (Physics, Novartis AG) for encouragement, and for allowing us use of their NMR equipment during all stages of this project; J.L. Markley and T. Schirmer for their comments on the manuscript. This work was supported by grants from EU program Biotechnology II to J.E., and from the Swiss NF to J.E. and A.T.A.

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Authors and Affiliations

  1. Department of Structural Biology, Biozentrum, University of Basel, Basel, CH-4056, Switzerland

    Sonja A. Dames

  2. Department of Biophysical Chemistry, Biozentrum, University of Basel, Basel, CH-4056, Switzerland

    Richard A. Kammerer

  3. Department of Structural Biology, Biozentrum, University of Basel, Basel, CH-4056, Switzerland

    Ronald Wiltscheck

  4. Department of Biophysical Chemistry, Biozentrum, University of Basel, Basel, CH-4056, Switzerland

    Jürgen Engel

  5. Department of Structural Biology, Biozentrum, University of Basel, Basel, CH-4056, Switzerland

    Andrei T. Alexandrescu

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  1. Sonja A. Dames
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  2. Richard A. Kammerer
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  5. Andrei T. Alexandrescu
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Correspondence to Andrei T. Alexandrescu.

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Dames, S., Kammerer, R., Wiltscheck, R. et al. NMR structure of a parallel homotrimeric coiled coil. Nat Struct Mol Biol 5, 687–691 (1998). https://doi.org/10.1038/90444

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