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The structure of a cypovirus and the functional organization of dsRNA viruses

Nature Structural Biology volume 6, pages 565–568 (1999)Cite this article

Abstract

Cytoplasmic polyhedrosis virus (CPV) is unique among the double-stranded RNA viruses of the family Reoviridae in having a single capsid layer. Analysis by cryo-electron microscopy allows comparison of the single shelled CPV and orthoreovirus with the high resolution crystal structure of the inner shell of the bluetongue virus (BTV) core. This suggests that the novel arrangement identified in BTV, of 120 protein subunits in a so-called 'T=2' organization, is a characteristic of the Reoviridae and allows us to delineate structural similarities and differences between two subgroups of the family — the turreted and the smooth-core viruses. This in turn suggests a coherent picture of the structural organization of many dsRNA viruses.

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Figure 1: Cryo-micrographs of CPV-5 virions used for image processing and three-dimensional reconstruction.
Figure 2: Surface representation of the non-occluded CPV-5 reconstruction.
Figure 3
Figure 4: Comparison of CPV-5 and orthoreovirus.
Figure 5: Close up of CPV-5 looking down an icosahedral three-fold axis of the particle with cartoon representations of the size and arrangement of the inner shell protein of BTV (VP3(T2)) marked in green (molecule A) and red (molecule B), derived from data in ref. 14.

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Acknowledgements

This work was supported by grants from the BBSRC and EC, an NERC industry-targeted studentship to C.L.H., sponsored by Oxford Instruments Plc. P.M. is supported by MAFF, D.I.S. by MRC and B.V.V.P. acknowledges support from the NIH. We thank T. Baker for kindly providing the electron density reconstructions for the orthoreovirus core and virion.

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Author notes

  1. Claire L. Hill

    Present address: Second Floor Laboratories, Jefferiss wing, Imperial Medical School at St Mary's, Praed Street, London, W2 1NY, UK

  2. Timothy F. Booth

    Present address: Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, 1051 Arlington Street, Winnipeg, Manitoba, R3E 3M4, Canada

Authors and Affiliations

  1. NERC Institute of Virology and Environmental Microbiology, Mansfield Road, OX1 3SR, Oxford, UK

    Claire L. Hill & Timothy F. Booth

  2. Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, 1 Baylor Plaza, Houston, 77030, Texas, USA

    B. V. Venkataram Prasad

  3. Laboratory of Molecular Biophysics, Rex Richards Building, South Parks Road, Oxford, OX1 3QU, UK

    Jonathan M. Grimes, Geoff C. Sutton & David I. Stuart

  4. Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, UK

    Peter P. C. Mertens

  5. The Oxford Centre for Molecular Sciences, New Chemistry Laboratory, Oxford, OX1 3QT, UK

    David I. Stuart

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Correspondence to David I. Stuart.

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Hill, C., Booth, T., Prasad, B. et al. The structure of a cypovirus and the functional organization of dsRNA viruses. Nat Struct Mol Biol 6, 565–568 (1999). https://doi.org/10.1038/9347

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