Abstract
The crystal structure of the purine represser mutant L54M bound to hypoxanthine and to the purF operator provides a stereochemical understanding of the high DNA affinity of this hinge helix mutant. Comparison of the PurR L54M–DNA complex to that of the wild type PurR–DNA complex reveals that these purine repressers bind and kink DNA similarly despite significant differences in their minor groove contacts and routes to interdigitation of the central C·G:G·C base pair step. Modeling studies, supported by genetic and biochemical data, show that the stereochemistry of the backbone atoms of the abutting hinge helices combined with the rigidity of the kinked base pair step constrain the interdigitating residue to leucine or methionine for the LacI/GalR family of transcription regulators.
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References
Schumacher, M.A., Choi, K.Y., Zalkin, H. & Brennan, R.G. Science 266, 763–770 (1994).
Rolfes, R.J. & Zalkin, H. J. Biol. Chem. 263, 19653–19661 (1988).
Farabaugh, P.J. Nature 274, 765–769 (1978).
Lewis, M. et al. Science 271, 1247–1254 (1996).
Weickert, M.J. & Adhya, S. J. Biol. Chem. 267, 15869–15874 (1992).
Nguyen, C.C. & Saier, M.H.J. FEBS Lett. 377, 98–102 (1995).
Schumacher, M.A., Glasfeld, A., Zalkin, H. & Brennan, R.G. J. Biol. Chem. 272, 22648–22653 (1997).
Glasfeld, A., Schumacher, M.A., Choi, K.Y., Zalkin, H. & Brennan, R.G. J. Am. Chem. Soc. 118, 13073–13074 (1996).
Choi, K.Y. & Zalkin, H. J. Bacteriol. 176, 1767–1772 (1994).
Pace, H.C. et al. Trends Biochem. Sci. 22, 334–339 (1997).
Lu, F. et al. Biochemistry 37, 971–982 (1998).
Hagerman, P.J. Proc. Natl. Acad. Sci. USA 93, 9993–9996 (1996).
Lavory, R. & Sklenar, H. J. Biomol. Struct. Dynam. 6, 63–91 (1988).
Creamer, T.P. & Rose, G.D. Proc. Natl. Acad. Sci. USA 89, 5937–5941 (1992).
Sternberg, M.J. & Chickos, J.S. Prot. Engng. 7, 149–155 (1994).
Gassner, N.C., Baase, W.A. & Matthews, B.W. Proc. Natl. Acad. Sci. USA 93, 12155–12158 (1996).
Carugo, O. & Argos, P. Prot. Engng. 10, 777–787 (1997).
Spolar, R.S. & Record, M.T., Jr . Science 263, 777–784 (1994).
Nagadoi, A. et al. Structure. 3, 1217–1224 (1995).
Spronk, C.A., Slijper, M., van Boom, J.H., Kaptein, R. & Boelens, R. Nature Struct. Biol. 3, 916–919 (1996).
Horlacher, R. & Boos, W. J. Biol. Chem. 272, 13026–13032 (1997).
Bockmann, J., Heuel, H. & Lengeler, J.W. Mol. Gen. Genet. 235, 22–32 (1992).
Tucker-Kellogg, L. et al. Structure. 5, 1047–1054 (1997).
Valentin-Hansen, P., Larsen, J.E., Hojrup, P., Short, S.A. & Barbier, C.S. Nucleic Acids Res. 14, 2215–2228 (1986).
Pedersen, H. & Valentin-Hansen, P. EMBO. J. 16, 2108–2118 (1997).
Kirn, J.L., Nikolov, D.B. & Burley, S.K. Nature 365, 520–527 (1993).
Kim, Y., Geiger, J.H., Hahn, S. & Sigler, P.B. Nature 365, 512–520 (1993).
Kim, J.L. & Burley, S.K. Nature Struct. Biol. 1, 638–653 (1994).
Juo, Z.S. et al. J. Mol. Biol. 261, 239–254 (1996).
Kosa, P.F., Ghosh, G., DeDecker, B.S. & Sigler, P.B. Proc. Natl. Acad. Sci. USA 94, 6042–6047 (1997).
Reddy, P. & Hahn, S. Cell 65, 349–357 (1991).
Werner, M.H., Huth, J.R., Gronenborn, A.M. & Clore, G.M. Cell 81, 705–714 (1995).
Love, J.J. et al. Nature 376, 791–795 (1995).
Swaminathan, K., Flynn, P., Reece, R.J. & Marmorstein, R. Nature Struct. Biol. 4, 751–759 (1997).
Rice, P.A., Yang, S., Mizuuchi, K. & Nash, H.A. Cell 87, 1295–1306 (1996).
Schumacher, M.A., Choi, K.Y., Zalkin, H. & Brennan, R.G. J. Mol. Biol. 242, 302–305 (1994).
Tronrud, D.E., Ten Eyck, L.F. & Matthews, B.W. Acta. Crystallogr. A43, 489–501 (1987).
Jones, T.A., Zou, J.Y., Cowan, S.W. & Kjeldgaard, M. Acta. Crystallogr. A47, 110–119 (1991).
Kraulis, P.J. J. Appl. Crystallogr. 24, 946–950 (1991).
Sayle, R. & Milner-White, E.J. Trends Biochem. Sci. 20, 333–379 (1995).
Nicholls, A., Sharp, K.A. & Honig, B. Proteins 11, 281–296 (1991).
Laskowski, R.A., MacArthur, M.W. & Thornton, J.M. J. Appl. Crystallogr. 26, 283–291 (1993).
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Arvidson, D., Lu, F., Faber, C. et al. The structure of PurR mutant L54M shows an alternative route to DNA kinking. Nat Struct Mol Biol 5, 436–441 (1998). https://doi.org/10.1038/nsb0698-436
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DOI: https://doi.org/10.1038/nsb0698-436