Obtaining high-resolution structural and molecular information from intact brain tissue is a key goal, but existing techniques face several limitations. In a new approach, named CLARITY by the authors, an intact mouse brain tissue was hybridized with a hydrogel to preserve its structure and then stripped of lipids. The resulting sample was optically transparent and could be penetrated by a range of chemical and biological reagents, enabling the authors to visualize many structural and molecular features of the intact brain.
References
Chung, K. et al. Structural and molecular interrogation of intact biological systems. Nature 10 Apr 2013 (10.1038/nature12107)
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Whalley, K. CLARITY in imaging. Nat Rev Neurosci 14, 380 (2013). https://doi.org/10.1038/nrn3513
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DOI: https://doi.org/10.1038/nrn3513