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Testing the water: marine metagenomics

Abstract

This month's Genome Watch reviews three recent papers that describe metagenomic analyses of marine and freshwater bacteria.

Main

Although bacterial genomes are traditionally sequenced from cultured isolates, metagenomic analyses allow researchers to gain insights into the genomes of unculturable microorganisms. Marine habitats have been a particular target and two recent studies that build on our current knowledge of the 'marine metagenome' have applied the metagenomic principle in different ways1,2.

Woyke et al.1 used flow cytometry to extract live cells from a sample of coastal water. The DNA from 11 single cells was individually amplified using multiple displacement amplification (MDA) and two cells were identified as the proteorhodopsin-containing Flavobacteria bacterium MS024-2A and F. bacterium MS024-3C. Following a second round of MDA, DNA from the uncultured flavobacteria was used for both Sanger shotgun sequencing and 454 pyrosequencing. Final assemblies were 1.9 Mb (17 contigs) and 1.5 Mb (21 contigs), which are estimated to represent 91% and 78% of each genome, respectively. Both genomes were smaller in comparison to other sequenced Bacteroidetes, a feature that is thought to be a nutrient and energy conserving adaptation in marine alphaproteobacteria. Both genomes also encode genes for the hydrolysis of allophanate (a by-product of urea breakdown), which is a likely additional source of nitrogen. Unexpectedly, F. bacterium MS024-2A is unique among marine flavobacteria, as it encodes the nickel and iron hydrogenase genes hyaA and hyaB, which suggests that it can use hydrogen as an energy source.

Credit: Neil Smith

Palenik et al.2 also sampled coastal water but took an alternative approach, using the natural fluorescence of Synechococcus spp. with flow cytometry to enrich for fluorescing cells. Synechococcus, a cyanobacterial genus, is classified into four clades. The enriched collection of Palenik et al. was estimated to contain 20,000 cells from clade I and 50,000 cells from clade IV, which are the two clades that are typically present in the coastal environment. Sequence reads were generated by 454 pyrosequencing after whole genome amplification and were then mapped to the four complete Synechococcus genomes that are currently available. Although reads mapped to coastal reference strains 20 times more frequently than to open-ocean reference strains, reads tended not to map to regions of atypical nucleotide content, even in the coastal reference strains. This implies that these regions represent horizontally acquired genes, possibly as genomic islands, that are not conserved across Synechococcus spp. In particular, a large region of atypical nucleotide content in the Synechococcus sp. CC9902 reference genome is absent in the metagenome, indicating that this region is a possible hot spot for recombination of acquired DNA.

Three families of plasmids that represent genetic elements previously unseen in marine cyanobacterial genomes were also reconstructed from the sequence data. This raises the possibility that plasmids have a role in gene transfer in coastal Synechococcus genomes, which contain none of the genomic islands that are characteristic of the phage seen in open-ocean genomes such as Synechococcus sp. WH 8102. Multiple mechanisms for horizontal gene transfer might therefore be active in the Synechococcus genus.

Alongside these investigations into the marine metagenome, a collaborative study in Mexico has tackled the microorganisms living in freshwater 'microbialites', or matrices of exopolymeric substances3. A diverse range of heterotrophs and autotrophs were identified, and members of the cyanobacterial order Chroococcales (of which Synechococcus is a genus) were commonly found in morphologically distinct samples. However, the metagenome was enriched for genes involved in phosphorus metabolism and the establishment and development of biofilms, features that distinguish these freshwater microbialite communities from their marine counterparts.

Metagenomic analyses were initially used to gain a broad understanding of which species were present in a microbial community, and this remains the most common form of investigation. However, as described above, marine metagenomics is expanding the approaches that can be taken to gain a better understanding of unculturable organisms.

References

  1. 1

    Woyke, T. et al. Assembling the marine metagenome, one cell at a time. PLoS ONE 4, e5299 (2009).

    Article  PubMed  PubMed Central  Google Scholar 

  2. 2

    Palenik, B., Ren, Q., Tai, V. & Paulsen, I. T. Coastal Synechococcus metagenome reveals major roles for horizontal gene transfer and plasmids in population diversity. Environ. Microbiol. 11, 349–359 (2009).

    CAS  Article  PubMed  Google Scholar 

  3. 3

    Breitbart, M. et al. Metagenomic and stable isotopic analyses of modern freshwater microbialites in Cuatro Cienegas, Mexico. Environ. Microbiol. 11, 16–34 (2009).

    CAS  Article  Google Scholar 

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Entrez Genome Project

Flavobacteria bacterium MS024-2A

Flavobacteria bacterium MS024-3C

Synechococcus sp. CC9902

Synechococcus sp. WH 8102

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Langridge, G. Testing the water: marine metagenomics. Nat Rev Microbiol 7, 552 (2009). https://doi.org/10.1038/nrmicro2188

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