Key Points
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Neisseria meningitidis and Neisseria gonorrhoeae are human-specific pathogens and possess a range of mechanisms to achieve successful colonization of their unique niches. The bacteria are closely related genetically but have some important differences, one of which is the expression of capsule polysaccharides by most disease-causing N. meningitidis isolates but not by N. gonorrhoeae isolates.
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Their specificity for the host is partly achieved by specific host adhesion mechanisms. As adhesins are required for the first step of colonization, they also constitute key virulence factors and have been studied extensively.
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The Review examines several important aspects of the structures and functions of the well-known major adhesins, Opa, Opc and pili. In addition, the known target receptors are described and the potential role of receptor modulation in increasing host susceptibility to infection is considered.
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A signature property of the pathogens is constant surface variation, which helps immune avoidance and is achieved by specific genetic mechanisms such as slipped strand mispairing and genomic recombination events, as well as bacterial responses to environmental factors. The bacterial capsule, lipopolysaccharide and surface proteins are subject to modulation.
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Changes in the form of antigenic and phase variation of adhesins are compensated for by the presence of many mechanisms of adhesion and by conservation of adhesion function in structural variants of some proteins. The strategies of surface variation, host targeting and immune evasion, and the interplay of adhesins and surface polysaccharides are examined.
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Constant surface variation has posed problems in developing broadly protective vaccines to combat some problematic strains of N. meningitidis and all strains of N. gonorrhoeae. In the search for new vaccine antigens, genome mining studies have identified numerous potential candidates, which include several previously unknown minor adhesins, many of which are autotransporter molecules.
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Recent advances in the field also include a greater understanding of the mechanisms of host targeting and tissue invasion that are afforded by the major adhesins, such as the Opa proteins. In addition, few Opa variants predominate in meningococcal isolates and their receptor targeting function is largely conserved. These studies invite examination of such candidates for the development of strategies for the prevention of tissue infiltration.
Abstract
Although renowned as a lethal pathogen, Neisseria meningitidis has adapted to be a commensal of the human nasopharynx. It shares extensive genetic and antigenic similarities with the urogenital pathogen Neisseria gonorrhoeae but displays a distinct lifestyle and niche preference. Together, they pose a considerable challenge for vaccine development as they modulate their surface structures with remarkable speed. Nonetheless, their host-cell attachment and invasion capacity is maintained, a property that could be exploited to combat tissue infiltration. With the primary focus on N. meningitidis, this Review examines the known mechanisms used by these pathogens for niche establishment and the challenges such mechanisms pose for infection control.
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Main
Neisseria meningitidis (meningococcus) and Neisseria gonorrhoeae (gonococcus), the well known agents of epidemic meningitis and gonorrhoea, respectively, are related Gram-negative bacteria that specifically infect humans; both pathogens prefer to inhabit distinct human mucosal niches and cause markedly different diseases (Fig. 1). One important difference between the pathogens is that almost all clinically important N. meningitidis strains are encapsulated, whereas N. gonorrhoeae strains lack capsule biosynthetic genes. N. meningitidis is a frequent asymptomatic colonizer of the human upper respiratory tract, and most adults are resistant to infection through acquired immunity. However, in susceptible individuals N. meningitidis can cause serious blood and brain infections that are usually manifested as meningitis and septicaemia. It also seems that meningococcal strains vary in their ability to cause sporadic or epidemic outbreaks. The outcomes of meningococcal infection may be devastating and, in the absence of timely intervention, can lead to neurological disorders and death1. N. gonorrhoeae is a sexually transmitted pathogen that primarily infects the urogenital tract, giving rise to intense local inflammation and a range of clinical manifestations2. A signature property of the two pathogens is their ability to modulate their surface antigenic make up with remarkable speed. This is the basis of their success as human-specific pathogens, as constant surface modulation3,4,5 and point mutations6 enable the bacteria to evade human immune mechanisms. Extensive surface variation also poses a substantial problem in developing effective vaccines against several strains of N. meningitidis and against N. gonorrhoeae. Although multicomponent vaccines are being developed, the available vaccines fall short of combating all virulent strains7,8.
An array of molecules is produced by bacteria to enable them to colonize and/or infect the host, including adhesins, which are key factors that are required for initial colonization of human mucosal sites. Characteristically, pathogens can modulate the expression and structure of adhesins and still maintain the ability to bind to mucosal epithelial cells for colonization. This might suggest some degree of structural conservation, a property that could be exploited for the prevention of infection. To achieve this aim, a thorough understanding of the range of host targeting strategies of the pathogens and of host factors that increase susceptibility to infection is needed. This Review describes the scale of the problem, focusing on our current understanding of key aspects of the pathogenic tactics of Neisseria spp., particularly cellular adhesion and invasion mechanisms. I also discuss the relationship between colonization and immune evasion strategies and address host susceptibility in the context of adhesion receptors. As carriage is itself considered an immunizing event that helps maintain long-term memory, the approaches that could control infection without eliminating colonization are also discussed.
Antigenic relatedness and carriage
Together with 17 other species, N. meningitidis and N. gonorrhoeae belong to the genus Neisseria9. Most species within the genus are classified as true human commensal bacteria and have negligible infection rates. The best recognized species of this group is Neisseria lactamica , which shares the human respiratory niche and antigenic structures with N. meningitidis. The highest carriage rate of N. lactamica occurs in early childhood and has been associated with the development of a cross-protective immunity against N. meningitidis10. Meningococcal carriage rate increases gradually after birth and reaches a peak in teenagers, with the average carriage rate being about 10% of the population in the United Kingdom1. Carriage rates tend to be high in institutional settings, for example, in military recruits and university students11. Thus, nasopharyngeal colonization with N. lactamica or other non-pathogenic Neisseria strains does not seem to protect against N. meningitidis carriage, but does protect against N. meningitidis infection. Indeed, infection rate with N. meningitidis is considerably lower (1–5 per 100,000 individuals in Europe) than carriage rate. In addition, infection rate is often associated with increased susceptibility in immunocompromised hosts1 (for example, following splenectomy or exposure to enteric pathogens that give rise to a cross-reacting but blocking IgA response) or in genetically predisposed hosts (particularly those with antibody and complement deficiencies)11,12. Other factors that may contribute to host susceptibility are considered below.
Meningococcal disease is a worldwide problem and is endemic in most countries. In endemic situations, it is prevalent in two age groups: children under 1 year of age and young adults between 15–19 years of age. In addition, periodic epidemics occur in Sub-Saharan Africa, especially in the 'meningitis belt' (Ref. 13).
Compared with N. meningitidis, which spreads by respiratory aerosol droplets and can infect those in close proximity, the gonococcal mode of transmission limits the population at risk. However, after sexual contact with an infected partner, the risk of female infection is much greater than the risk of male infection, aided partly by the ability of N. gonorrhoeae to bind to human sperm2. Urogenital surfaces are the primary sites of infection by N. gonorrhoeae, although other sites may also become involved. Gonococcal infections are usually localized and elicit an intense inflammatory response that gives rise to purulent discharge in male patients, a hallmark of gonorrhoea. In females, the different embryological origin of the urogenital tract results in a different mode of infection, which is often asymptomatic (reviewed in Ref. 2).
Virulence genes of neisseriae
Complete nucleotide sequences of several pathogenic Neisseria strains and of N. lactamica (some of which have been available for almost a decade) have facilitated the identification of numerous previously unknown putative adhesins and virulence factors14,15,16 (also see the Sanger Institute Neisseria lactamica website and the University of Oklahoma Neisseria gonorrhoeae Genome Sequencing Strain FA website). A number of islands of horizontally transferred DNA have been found in the genome of N. meningitidis. However, no classic organized pathogenicity islands are present that define the virulent behaviour of the organisms17. Instead, the N. meningitidis genome has 'genetic islands' with identifiable genes that differ in their GC content and codon usage, which have been acquired through horizontal exchange with other mucosal bacteria14,17. Free exchange between genes (gene conversion) both within and between the genomes of Neisseria spp.3 is a prominent mechanism for the acquisition of new traits and is facilitated by the natural competence of Neisseria species. Neisserial DNA in the environment is believed to arise by autolysis and by a recently identified N. gonorrhoeae type IV secretion system that actively transports DNA out of the cell18. Intergenomic recombination events tend to maintain a largely non-clonal population structure, although clonal clusters are clearly detectable in N. meningitidis by multilocus sequence typing (MLST). This has delineated several meningococcal hypervirulent lineages that are responsible for epidemics worldwide11,19.
Colonization and virulence factors
The key structures at the interface between the host and Neisseria spp. are the polysaccharide capsules and/or lipopolysaccharide (LPS) that may shield bacterial surfaces from the host innate and adaptive immune effector mechanisms, and the protruding surface proteins that are known as pili (hair-like projections; also known as fimbriae) (Fig. 2; Table 1). Pili facilitate adhesion to host tissues, further aided by the outer membrane adhesins, Opa and Opc, which are described below. At least 12 N. meningitidis LPS immunotypes, designated L1–L12, have been identified on a serological basis. The notable immunotypes are L3, L7 and L9, which can be sialylated, and L8, which lacks the terminal lacto-N-neotetraose (LNT) that is required for the addition of the sialic acid moiety20. Neisseria spp. also produce numerous secreted proteins (Box 1).
Specificity for the host, as well as for tissues within the host, is believed to be attained primarily through adhesins. Additionally, Neisseria spp. possess host-specific iron acquisition mechanisms and numerous immune evasion mechanisms21 (Box 2). Host specificity poses a problem for developing animal models of the disease, and as a result most of our knowledge of the pathogenic mechanisms of Neisseria spp. comes from in vitro investigations.
Mechanisms of phase and antigenic variation
Aside from the gene conversion events mentioned above, other mechanisms operate in Neisseria spp. to give rise to phase variation and structural or antigenic variation. Phase variation occurs primarily through the process that is commonly referred to as slipped strand mispairing (SSM) and involves DNA slippage induced by repetitive sequences of nucleotides within or upstream of genes. This results in translational control of expression, which can reversibly switch gene expression on and off4,20, or transcriptional control of expression, which can change the level of gene expression (as in the case of Opc)22. Antigenic variation can arise as a result of phase variation of one or more enzymes involved in LPS biosynthesis20 or of distinct Opa proteins, as described below. In the case of PilE (pilin), the main subunit that makes up the pilus fibre, variations arise from intergenomic and intragenomic recombinase A-dependent recombination events between one of several pilS (silent) pilin genes and pilE, the expressed pilin gene3,23.
Redundancy, antigenic and phase variation. The major adhesins (pili and Opa), which enable anchorage to host tissues, have been long recognized in N. meningitidis and N. gonorrhoeae. In addition, Opc expressed in N. meningitidis, but not N. gonorrhoeae24, is also an important adhesin (Fig. 2). Numerous additional apparently minor adhesins (several of which were identified by homology searching of the available genomes) are generally expressed at low levels in vitro but may be important in vivo. For example, in restricted iron environments, such as might be encountered in vivo, the transcriptome of N. meningitidis is considerably altered25 and as a result the minor adhesins may become expressed. Furthermore, several adhesins are subject to antigenic variation and/or phase variation, which can reach high frequencies and may vary between strains (see N. gonorrhoeae pilin variation rates26). Surface modulation facilitates evasion of immune effector mechanisms but can require multiplicity of adhesins (redundancy) to maintain colonization. Several adhesins may also operate simultaneously to increase the avidity of bacterial binding to the cell surface. This is often a prelude to internalization into epithelial cells27,28, which is another immune evasion strategy.
Such constant variation renders most important surface components unsuitable as vaccine candidates. However, structures that are required for survival in vivo (for example, capsules, which are further described below) have been used successfully to protect against several virulent N. meningitidis strains. In other cases, such as in the case of the Opa protein family, the frequency of expression, abundance and functional conservation (and therefore a degree of structural conservation) suggests they might be appropriate vaccine candidates29,30. These observations highlight the need for in-depth studies on the structure–function relationship of members of the Opa family; understanding their modes of action, for example, their mechanisms of host-cell receptor targeting, could lead to intervention strategies to prevent infection.
Surface sialic acids, pathogenicity and modulation of adhesion. The capsule is a highly hydrated structure and is thought to protect meningococci during airborne transmission between hosts31. Once in the respiratory tract, meningococci may become non-encapsulated through numerous genetic mechanisms32,33. One of these mechanisms may involve the induction of crgA, a gene that is upregulated on contact with target cells and the product of which is a transcriptional regulator of several genes, including those that are involved in capsule biosynthesis33. Even though many bacterial isolates from the nasopharynx are non-encapsulated, disseminated infections are almost always caused by encapsulated bacteria. The capsule can prevent antibody and complement deposition22, it is anti-opsonic and anti-phagocytic and it aids survival in the blood. Indeed, the serogroup B capsule has been shown to inhibit serum immunoglobulin G (IgG) deposition and, perhaps consequently, complement deposition on the bacteria (S. Ram, personal communication). High levels of capsule expression can also inhibit complement-mediated lysis in the presence of bactericidal antibodies that are specific for PorA or are raised against whole cells34.
One of 13 distinct capsular structures can be expressed by meningococcal strains and form the basis for the classification of N. meningitidis into different serogroups. Disease is caused most frequently by strains of serogroups A, B and C, followed by W135 and Y; the other serogroups rarely cause disease1. Four of these serogroup capsules contain sialic acid (Table 1), which is important for immune evasion35. As they are the outer-most structures of the bacterium and because of their importance in disseminated infections, capsules are good vaccine candidates. Current capsule-based vaccines against N. meningitidis target specific serogroups (A, C, Y and W135). However, serogroup B remains a problem for vaccine design, as it is not an effective immunogen owing to its structural similarities with glycans on human neuronal-cell adhesion molecules (Table 1).
Sialic acids are also present on the LPS of both N. meningitidis and N. gonorrhoeae. In vitro studies have shown that the addition of sialic acids to LPS can impart capsule-like properties to LPS, making the bacteria more resistant to antibody and complement-mediated killing and more able to avoid phagocytosis. Both LNT and sialylated LPS also mimic host-cell surface structures, which facilitates avoidance of the host antibody response2,35,36,37. However, (α2,3)-linked sialic acid on the LPS of N. meningitidis is recognized by sialic-acid-binding immunoglobulin-like lectins (Siglecs), which are present on some phagocytic cells. Thus, the expression of LPS sialic acids can potentially render bacteria more susceptible to phagocytosis38; the in vivo importance of this is not known. N. gonorrhoeae phenotypes that have unsialylated LPS use another host receptor, the asialoglycoprotein receptor (ASGPR), to interact with host urethral epithelial cells2.
Meningococcal LPS is responsible for eliciting inflammation during sepsis39 and is also highly toxic for human endothelial cells in vitro. This property is augmented in the presence of pili, suggesting that the two components cooperate in signalling to endothelial cells40. Interestingly, co-signalling of human endothelial cells by the pili and LPS of N. meningitidis was recently reported, and this results in bacterial uptake by non-phagocytic cells41.
Neisserial strains that harbour genes for the synthesis of sialic-acid-containing capsules can generate an endogenous source of sialic acid and can add this moiety to LNT of LPS through LPS sialyl transferase, which is present in strains of both N. meningitidis and N. gonorrhoeae. However, N. meningitidis serogroup A strains and N. gonorrhoeae do not synthesize sialic acid and therefore require an exogenous source for this purpose. Indeed, during infection, they acquire sialic acid from host fluids37. Thus, the surface of N. meningitidis and N. gonorrhoeae can be either devoid of, or encased in, one to several layers of negatively charged molecules that are provided by the capsule and/or sialylated LPS.
In addition to inhibiting opsonization-mediated phagocytosis and detection by complement, surface glycans can inhibit the function of non-pilus outer membrane adhesins and invasins by their juxtaposition and by charge neutralization, and thus are also anti-adhesins. As a result, non-encapsulated bacteria and those lacking sialic acids on LPS are the most invasive36,42. However, to cause disseminated disease, N. meningitidis requires the protection that is provided by surface sialic acids, especially those that are present in the capsule13,32. Therefore, one possible sequence of events during dissemination from the site of colonization is that capsule expression is switched from on to off and then on again to survive in the blood (Box 3).
Major adhesins of pathogenic Neisseria spp.
Of all the putative adhesins that have been identified so far, pili, Opa and Opc are expressed in the greatest abundance. Comparison of in vitro observations shows important quantitative differences between the interactions that are mediated by the major adhesins and several newly identified minor adhesins. The major adhesins also have the tendency to enhance bacterial self-agglutination, a phenomenon that influences bacterial adhesion levels.
Pili, the polymeric pericellular glycoproteins. Recent systematic genetic analyses have identified 15 proteins that are involved in the biogenesis, assembly and disassembly of pili (known as Pil proteins), and have begun to assign precise roles to them43. The pilus fibre consists of numerous PilE (major pilin) subunits arranged in a helical configuration. In addition, several minor subunits (PilC, PilV and PilX) can be incorporated in the fibre and modulate its function. Neisserial pilins undergo several distinct post-translational modifications, such as glycosylation44,45 (reviewed in Ref. 46), which can indirectly have an effect on cellular interactions, perhaps by affecting the agglutination of pili44,47. It has also been suggested that in N. meningitidis glycosylation is required to produce S pilins, which are truncated soluble pilin subunits that are not assembled but are secreted and could have a function in immune diversion and/or adhesion48. In N. meningitidis, the pgl (6-phosphogluconolactonase) gene cluster controls the modification of pili with glycans, the structure of which is determined by polymorphisms in pilin glycosylation genes and phase variation of glycosylation enzymes49,50,51. Whether this property has a role in increasing bacterial pathogenic potential is unsubstantiated.
Several modifications of pilin at serine 68 have been described. This residue may be modified with phosphoethanolamine or phosphorylcholine, or may remain unmodified52,53. These modifications alter the charge at this position, thus potentially affecting cellular interactions and immune recognition. Phosphorylcholine has been proposed to be a potential broadly effective vaccine candidate, as it is present on surface components of many mucosal organisms. The roles attributed to phosphorylcholine in various pathogens include mimicry of platelet activating factor (PAF), enabling binding to host cells through the PAF receptor and neutralizing the functions of host cationic antimicrobial peptides54. Interestingly, in commensal Neisseria spp. phosphorylcholine occurs on LPS and not on pili. In this case, incorporation of phosphorylcholine seems to involve the lic1 locus, which resembles that of Haemophilus influenzae and is absent from pathogenic Neisseria species55,56. In pathogenic Neisseria spp., phosphoethanolamine and phosphorylcholine can be incorporated by a single pilin phospho-form transferase enzyme (PptA). Moreover, PilV negatively modulates pilus modification by reducing the addition of both phosphoethanolamine and phosphorylcholine to pili in an as yet unresolved manner52,57,58.
The structure of the N. gonorrhoeae pilin was determined in 1995 (Ref. 45), and a new high resolution structure has been derived recently by a combination of the crystal structure of the pilin subunit and a three-dimensional cryo–electron microscopy reconstruction of the pilus filament59. In the assembled pili, the variable domains and the post-translational modifications are exposed on the surface (Fig. 2). Furthermore, the pilus contains alternative patches of positively and negatively charged regions. Glycans and phosphoethanolamine lie within negative patches in the assembled pili, and therefore their variations (phase or antigenic) are likely to modulate the adhesion properties of the pilus59.
It thus seems that pili maintain little structural conservation to allow host immune recognition, and past vaccine trials (in which the vaccine was based on the pilus structure) showed no protection against heterologous challenge60. However, minor conserved pilins, such as PilX, that function indirectly to increase host-cell targeting by increasing adhesion between bacteria might be useful vaccine antigens61.
In addition to adhesion, pili are involved in several other functions. For example, they facilitate uptake of foreign DNA from the extracellular milieu, thereby increasing the transformation frequency of bacteria and maintaining the genetic diversity that underpins the success of Neisseria spp. in the human host62. The pili of both N. gonorrhoeae and N. meningitidis are dynamic, as they can assemble and disassemble rapidly, and this is facilitated by the coordinate action of PilC and the ATPase PilT, resulting in 'twitching motility' (Ref. 65). By this process, a level of movement on cell surfaces of around 1 mm per second may be attained64. Extension and cellular attachment followed by retraction or disassembly of the pili may decrease the distance between the bacterial and eukaryotic membranes, thereby enabling the uptake of DNA and intimate cellular interactions through integral outer membrane adhesins. The considerable mechanical force that is generated by the process of pilus retraction may also be responsible for numerous signal transduction events, including the formation of cortical plaque structures and the shedding of the complement regulatory factor CD46, mediated by unknown cell-surface receptors63,65.
The opacity proteins. The Opa proteins of Neisseria spp. (initially termed PII or class 5 proteins) impart opacity to colonies that express the proteins66. N. meningitidis has an additional opacity protein, Opc. In N. meningitidis, colony opacity can only be seen clearly in non-encapsulated bacteria67 (Fig. 3). Opa proteins are a family of related transmembrane molecules that form eight-stranded β-barrel structures in the outer membrane of the bacterium with four surface-exposed loops (Fig. 2). Extensive structural variation occurs both within and between N. meningitidis strains in three of these loops. Furthermore, a single strain may express one to several Opa proteins, and alternate phase on/off of distinct opa genes can also give rise to antigenic variation. In addition, homologous recombination can increase the repertoire of Opa structures in a population4,68. Immunodominant regions of Opa proteins are contained within the variable regions of the protein, and as bactericidal antibodies elicited in the host are specific for the bacterial Opa type, their efficacy as cross-protective antigens is limited69. Adhesion-blocking antibodies have been generated by immunisation with purified Opa proteins, but these had no significant bactericidal or opsonic activity70. However, it seems that specific sets of Opa variants are prevalent in N. meningitidis isolates30. Besides immunological selection, such a repertoire of Opa proteins could arise as a consequence of functional constraints, perhaps directed by their ability to bind to carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1; discussed below). This suggests that Opa proteins could serve as potential vaccine components30.
N. meningitidis Opc (OpcA) is encoded by a single gene and does not vary greatly in structure22,24. Expression of Opc has not been shown in N. gonorrhoeae, and in fact certain N. meningitidis clonal lineages, such as ET37 complex, lack opcA71 and tend to cause severe sepsis instead of meningitis72,73,74. These observations have led to the speculation that Opc might be important in N. meningitidis-induced meningitis72.
Newly identified adhesins
Several minor adhesins or adhesin-like proteins have recently been described as a result of genome mining for the identification of new vaccine candidates. Their properties are summarized in Table 2.
NhhA and App. Both Neisseria hia homologue A (NhhA) and adhesion penetration protein (App) resemble the H. influenzae autotransporter proteins Hsf/Hia and Hap, respectively. NhhA is found in most disease-causing N. meningitidis isolates, but is absent from N. gonorrhoeae75,76. It mediates low levels of adhesion to epithelial cells, to heparan sulphate proteoglycans (HSPGs) and to laminin76. App is present in all neisserial genomes that have been sequenced, including commensal Neisseria species. It has been implicated in regulating interactions between the bacteria and the host tissue by mediating adhesion during the early stages of colonization, before it is autocleaved. At later stages, App autocleavage may allow bacterial detachment, therefore facilitating bacterial spread77.
HrpA–HrpB system. Recently, a two-partner secretion system, haemagglutinin/haemolysin-related protein A (HrpA)–HrpB, has been found in all strains of N. meningitidis. HrpA is the secreted effector protein and HrpB is the transporter component. A small proportion of HrpA remains associated with the outer membrane of N. meningitidis and according to one study contributes to bacterial adhesion to some epithelial cell lines78,126.
NadA and MspA. Neisserial adhesin A (NadA) belongs to the oligomeric coiled-coil (Oca) family of adhesins and seems to be more commonly associated with disease isolates than with carriage isolates and can mediate cellular adhesion79. Different alleles of the gene are found in three out of four hyper-virulent N. meningitidis lineages, but is largely absent from carrier strains and is not found in N. gonorrhoeae. The level of NadA expression may vary with the phase of bacterial growth and by SSM, as NadA contains tetra-nucleotide repeats (TAAA) in its promoter region80. NadA is expressed in several hyper-virulent lineages and is a proposed vaccine candidate against serogroup B N. meningitidis because it induces protective immune responses81.
Meningococcal serine protease A (MspA) is also expressed by several but not all virulent Neisseria strains. It is reported to mediate binding to both epithelial and endothelial cells and to elicit the production of bactericidal antibodies82.
Adhesion receptors and targeting mechanisms
Pili. As the capsule may protect N. meningitidis from desiccation during transmission between hosts, the organisms that are first encountered by the host are likely to be encapsulated. In such encapsulated organisms, the juxtaposition of the capsule masks many of the non-pilus adhesins and can render them functionally ineffective42,67,83. Therefore, pili are thought to have a crucial role in the initial establishment of encapsulated N. meningitidis on mucosal surfaces, facilitating penetration of the negatively charged barrier at the host–pathogen interface84. However, their targeted sites on mucosal surfaces are not random. For example, the pili of N. meningitidis interact with non-ciliated cells of the respiratory epithelium but do not interact with ciliated respiratory cells85.
In general, in vitro studies show little binding of N. meningitidis or N. gonorrhoeae pili to non-human cells44,86. Pili are also thought to be primary determinants of specificity for human epithelial and endothelial cells44,87 and those of N. meningitidis are known to mediate adhesion to cells of the human meninges88. In addition to this, binding to various other human cells has been demonstrated, including colonic cells86 and erythrocytes; in the case of erythrocytes, attachment is thought to be mediated primarily by PilE85,89.
Pilus receptors. Binding of the pili of N. meningitidis and N. gonorrhoeae to host cells is thought to involve CD4690, but not all studies support this observation87,91. N. gonorrhoeae pili may also bind to complement component C4 binding protein (C4BP) and complement receptor 3 (CR3; also known as αM-integrin). Binding to C4BP could be important for serum resistance, and binding to CR3 aids colonization of the cervical epithelium with the help of porins2,92.
Opacity protein receptors. As basic proteins, Opa and Opc use some common host-cell receptors when they target negatively charged structures on host-cell surfaces; these include HSPG and sialic acids93,94. The interaction between opacity proteins and sialic acids on LPS (which is also possible) can interfere with the recognition of target-cell receptors, which explains why there are reduced host–bacterium interactions following LPS sialylation94. HSPGs are targeted by a few N. meningitidis Opa proteins and several N. meningitidis Opa proteins tested93,95,96. HSPGs can also be used as receptors by the N. meningitidis Opc protein97,98. Opc may also directly bind to extracellular matrix (ECM) proteins, such as vitronectin and fibronectin72,99. As HSPGs interact with many ECM proteins, binding to HS molecules or ECM proteins introduces a complex array of molecular interactions between bacteria and the target cell100. Opc interactions with serum factors such as vitronectin and fibronectin leads to bacterial binding to endothelial αVβ3-integrin (the vitronectin receptor) and α5β1-integrin (the fibronectin receptor)42,72,99. This seems to be the main mode of interaction between Opc and polarized human endothelial cells (Fig. 3). Once inside the cells, N. meningitidis could escape the phagocytic vacuole126 and has been shown to bind to intracellular alpha-actinin through the Opc protein127.
In addition to the targets mentioned above, >90% of isolates of N. meningitidis and N. gonorrhoeae were shown to bind to CEACAM1. CEACAMs belong to the immunoglobulin superfamily29 (Fig. 4) and include several members (for example, CEACAM1, CEA and CEACAM3) of which CEACAM1 is the most widely distributed101. The binding sites of Opa proteins reside on the amino-terminal domains of the CEACAM family, which are largely conserved and therefore allow one or more Opa proteins to target several distinct CEACAMs29,102,103. As CEACAMs may contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or immunoreceptor tyrosine-based activation motifs (ITAMs)101, the consequences of downstream signalling following bacterial ligation depend on the receptor and target cell involved. From studies so far, it can be concluded that Opa–CEACAM interactions result in cellular invasion28,96,102.
Overall, it seems that tissue tropism may be influenced by pili, whereas host specificity may be determined by pili as well as Opa, as both seem to bind only to human receptors. As discussed above, the main receptors for the opacity proteins are known, but the identity of pilus receptors remains unclear. Further studies are needed to clarify the nature of molecules that are targeted by pili, especially in the case of N. meningitidis, as this will facilitate the generation of appropriate transgenic animal models of the disease. Future studies also need to use primary respiratory epithelial cells to assess the importance of identified receptors and indeed their precise distribution and levels of expression at various sites during health and disease. Another area of potential host-cell entry, the M cells of nasal epithelium-associated lymphoid tissue104, have also not been studied in detail for their role in neisserial transport.
Host susceptibility
Aside from the bactericidal capacity of the host, several other factors may contribute to increased host susceptibility to meningococcal infection, and may include several genetic polymorphisms105. Epidemiological studies also suggest that other compromising factors may contribute to host susceptibility, including physical damage to the mucosa that may ensue during respiratory infections (for example, viral infections in the winter months in the United Kingdom), dry atmospheric conditions (for example, in dry seasons in Africa) and smoking1. In addition, in several studies, synergism between specific viral and bacterial infections has been observed1,106. As meningococcal infection is not concurrent but follows influenza virus infection after a lag period1, it would seem that changes induced by viral infection other than physical damage could account for increased N. meningitidis infections. In this context, remodelling of mucosal tissues through upregulation of epithelial receptors by virus-induced cytokines might be potential determinants of enhanced bacterial adhesion and host-cell invasion.
CEACAM-density-dependent modulation of invasion. CEACAM1 can be upregulated in response to inflammatory cytokines28,102,107. This upregulation seems to increase Opa-mediated binding and invasion of fully encapsulated bacteria to human epithelial cells, a phenomenon that is aided by pili28. This is in contrast to observations on unstimulated cells that have low receptor density. It is possible that after initial host-cell–bacterium interactions through pili, bacterial and host-cell membranes are in close enough proximity for Opa and CEACAMs to engage, but further intimate interactions are inhibited by the capsule at low receptor expression levels. When receptor density is high, which would increase the functional affinity of the Opa–CEACAM interaction, such inhibition may be overcome. This provides a possible scenario in which hosts are rendered susceptible to invasion by virulent phenotypes following certain viral infections.
Natural and artificial anti-adhesion and anti-invasion measures. In contrast to the transmembrane CEACAM1, targeting of CEA, a glycosylphosphatidylinositol-anchored receptor, could lead to prevention of bacterial interactions with mucosal epithelial cells108. CEA is targeted by several gut pathogens through its mannosyl residues. It is shed in mg amounts daily in the gut and consequently has been proposed to form part of the innate immune response as it can act as a natural blocking agent for pathogen attachment in the gut101,108. Interestingly, CEA is also found in abundance on squamous epithelial cells of the tongue and oesophagus101, and on buccal (M.V. and N.J. Griffiths, unpublished observations) and cervical epithelial cells101. If CEA is shed from these cells as well, its presence at these tissues may also be regarded as a host strategy to prevent neisserial adhesion to the tissues. However, fewer Opa proteins target CEA compared with CEACAM1, a property that may reflect the evolutionary arms race between the pathogen and the host.
In addition to the Opa proteins of N. meningitidis, two unrelated adhesins, P5 and UspA1, of the mucosal pathogens H. influenzae and Moraxella catarrhalis , respectively, have been shown to bind primarily to CEACAM1 (Refs 109,110). As their binding sites on CEACAM1 overlap, the bacteria can compete for binding to the receptor; this has been shown in vitro. Opa and P5 are β-barrel proteins and their binding to CEACAM1 seems to involve several regions on the proteins. UspA1 belongs to the Oca family of proteins, and a recombinant molecule (rD-7) based on the structure of UspA1 has been developed that can bind CEACAM1. Moreover, rD-7 has the ability to block the interactions of all three mucosal pathogens111,112. Importantly, it can significantly inhibit Opa–CEACAM1-mediated cellular invasion of the encapsulated bacteria while not eliminating pilus-mediated adhesion, which occurs through a different receptor. This also occurs in the post-inflammation models of infection in which the density of CEACAM1 on the surface of target cells is enhanced, thereby supporting high levels of cellular invasion28 (Fig. 4).
Challenges for infection control
Meningococci excel at host adaptation. Their many adaptation mechanisms to the changing host environment pose a sizeable problem in the quest for a vaccine that will not become redundant as the bacterium develops new mechanisms to avoid host immunity. Notably, capsule switching between N. meningitidis of distinct serogroups has been observed in the course of natural colonization and in vaccinated individuals113. It is generally accepted that future successful vaccines will comprise several bacterial antigens. Such vaccines have been developed to cover the repertoire of circulating virulent strains of the bacteria8 and are needed to guard against the emergence of new resistant phenotypes. One clear strategy for lasting protection would be to reduce or eliminate the reservoir of the bacterium from the human population. To this end, vaccines that eliminate adhesion and induce herd immunity would be particularly beneficial. However, elimination of normal commensals may encourage other more aggressive pathogens to colonize the host. Is this a serious problem for a commensal that is transient and has a low to moderate carriage rate? Although this remains to be fully evaluated, herd immunity is a notable factor in the reduction of serogroup C disease in the United Kingdom following the introduction of the meningococcal serogroup C vaccine114.
An argument in favour of maintaining a level of N. meningitidis carriage is the notion that carriage is itself an immunizing event that helps to maintain long-term immunological memory. In this context, other choices may be available for infection control as specific blocking of certain interactions between adhesins and host-cell receptors at the mucosa could prevent tissue entry without eliminating carriage. The primary meningococcal invasins, Opa and Opc, and their cognate portals of cell entry, CEACAMs and integrins, could therefore be targeted specifically to interfere with the crucial step of host-cell penetration. In the case of some enteric bacteria, receptor mimics have proved effective for controlling infections (reviewed in Ref. 115). However, in the respiratory tract, adhesin and receptor analogues could be challenging to administer. Furthermore, it is currently unknown whether receptor-blocking agents can be generated to specifically prevent bacterial infiltration without interfering with the physiological functions of the receptor and without any potential side effects. Alternatively, peptides corresponding to adhesion domains could be used as vaccine antigens to induce blocking antibodies. This has been shown for the recombinant molecule rD-7, as the antibodies to adhesion domain of UspA1 prevented the interaction between M. catarrhalis UspA1 and CEACAM1 (Ref. 111). Another consideration, befitting the variable nature of the pathogen, is the possibility that other invasins that are at present not fully recognized could be upregulated in vivo. It is clear that more research will be required to identify any other key invasins of N. meningitidis, and studies so far have been hampered by the lack of a good model to study meningococcal pathogenesis.
References
Cartwright, K. Meningococcal Carriage and Disease in Meningococcal Disease (ed. Cartwright, K.) 115–146 (John Wiley & Sons, Chichester, 1995).
Edwards, J. L. & Apicella, M. A. The molecular mechanisms used by Neisseria gonorrhoeae to initiate infection differ between men and women. Clin. Microbiol. Rev. 17, 965–981 (2004). Presents recent advances in our understanding of the mechanisms of gonococcal pathogenesis in the context of the male and female human urogenital and genital tracts.
Segal, E., Hagblom, P., Seifert, H. S. & So, M. Antigenic variation of gonococcal pilus involves assembly of separated silent gene segments. Proc. Natl Acad. Sci. USA 83, 2177–2181 (1986).
Stern, A., Brown, M., Nickel, P. & Meyer, T. F. Opacity genes in Neisseria gonorrhoeae: control of phase and antigenic variation. Cell 47, 61–71 (1986).
van der Woude, M. W. & Baumler, A. J. Phase and antigenic variation in bacteria. Clin. Microbiol. Rev. 17, 581–611 (2004). Provides an overview of the prevalence, mechanisms and importance of phase variation in bacteria.
McGuinness, B. T. et al. Point mutation in meningococcal porA gene associated with increased endemic disease. Lancet 337, 514–517 (1991).
Weynants, V. E. et al. Additive and synergistic bactericidal activity of antibodies directed against minor outer membrane proteins of Neisseria meningitidis. Infect. Immun. 75, 5434–5442 (2007).
Giuliani, M. M. et al. A universal vaccine for serogroup B meningococcus. Proc. Natl Acad. Sci. USA 103, 10834–10839 (2006).
Tønjum, T. AL in Book Bergey's Manual of Systematic Bacteriology Vol. Two (ed. Garrity, G. M.) 777–798 (Springer US, 2005).
Gold, R., Goldschneider, I., Lepow, M. L., Draper, T. F. & Randolph, M. Carriage of Neisseria meningitidis and Neisseria lactamica in infants and children. J. Infect. Dis. 137, 112–121 (1978).
Yazdankhah, S. P. & Caugant, D. A. Neisseria meningitidis: an overview of the carriage state. J. Med. Microbiol. 53, 821–832 (2004). Discusses meningococcal carriage in various contexts, including methodology, molecular epidemiology, genetic exchange, immune response elicited by carriage and effect of vaccination on carriage.
Gotschlich, E. C., Goldschneider, I. & Artenstein, M. S. Human immunity to the meningococcus. IV. Immunogenicity of group A and group C meningococcal polysaccharides in human volunteers. J. Exp. Med. 129, 1367–1384 (1969).
Stephens, D. S. Conquering the meningococcus. FEMS Microbiol. Rev. 31, 3–14 (2007). An overview of the current knowledge of meningococcal pathogenesis, epidemiology and basis of host susceptibility. It discusses new discoveries and their probable effect in the control and prevention of meningococcal disease.
Tettelin, H. et al. Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science 287, 1809–1815 (2000).
Parkhill, J. et al. Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Nature 404, 502–506 (2000).
Bentley, S. D. et al. Meningococcal genetic variation mechanisms viewed through comparative analysis of serogroup C strain FAM18. PLoS Genet. 3, e23 (2007).
Perrin, A. et al. Comparative genomics identifies the genetic islands that distinguish Neisseria meningitidis, the agent of cerebrospinal meningitis, from other Neisseria species. Infect. Immun. 70, 7063–7072 (2002).
Hamilton, H. L. & Dillard, J. P. Natural transformation of Neisseria gonorrhoeae: from DNA donation to homologous recombination. Mol. Microbiol. 59, 376–385 (2006). Describes recent developments in our understanding of the key steps of gonococcal transformation: DNA donation, uptake, processing and integration into the gonococcal chromosome.
Feil, E. J., Maiden, M. C., Achtman, M. & Spratt, B. G. The relative contributions of recombination and mutation to the divergence of clones of Neisseria meningitidis. Mol. Biol. Evol. 16, 1496–1502 (1999). Describes the genetic diversities present in some bacterial populations at and below the species level and discusses approaches for defining bacterial species, especially highly mutable bacteria.
Jennings, M. P. et al. The genetic basis of the phase variation repertoire of lipopolysaccharide immunotypes in Neisseria meningitidis. Microbiology 145, 3013–3021 (1999).
Rohde, K. H. & Dyer, D. W. Mechanisms of iron acquisition by the human pathogens Neisseria meningitidis and Neisseria gonorrhoeae. Front. Biosi. 8, D1186–D1218 (2003).
Achtman, M. Epidemic spread and antigenic variability of Neisseria meningitidis. Trends Microbiol. 3, 186–192 (1995).
Haas, R. & Meyer, T. F. The repertoire of silent pilus genes in Neisseria gonorrhoeae: evidence for gene conversion. Cell 44, 107–115 (1986).
Zhu, P. X., Morelli, G. & Achtman, M. The opcA and ψ opcB regions in Neisseria: genes, pseudogenes, deletions, insertion elements and DNA islands. Mol. Microbiol. 33, 635–650 (1999).
Grifantini, R. et al. Identification of iron-activated and -repressed Fur-dependent genes by transcriptome analysis of Neisseria meningitidis group B. Proc. Natl Acad. Sci. USA 100, 9542–9547 (2003).
Criss, A. K., Kline, K. A. & Seifert, H. S. The frequency and rate of pilin antigenic variation in Neisseria gonorrhoeae. Mol. Microbiol. 58, 510–519 (2005).
Tran Van Nhieu, G. & Isberg, R. R. Bacterial internalization mediated by beta 1 chain integrins is determined by ligand affinity and receptor density. EMBO J. 12, 1887–1895 (1993).
Griffiths, N. J., Bradley, C. J., Heyderman, R. S. & Virji, M. IFN-γ amplifies NFκB-dependent Neisseria meningitidis invasion of epithelial cells via specific upregulation of CEA-related cell adhesion molecule 1. Cell. Microbiol. 9, 2968–2983 (2007).
Virji, M., Watt, S. M., Barker, S., Makepeace, K. & Doyonnas, R. The N-domain of the human CD66a adhesion molecule is a target for Opa proteins of Neisseria meningitidis and Neisseria gonorrhoeae. Mol. Microbiol. 22, 929–939 (1996). First demonstration of the targeting of CEACAM1 by diverse Opa types of clinical isolates of N. meningitidis and N. gonorrhoeae.
Callaghan, M. J., Jolley, K. A. & Maiden, M. C. Opacity-associated adhesin repertoire in hyperinvasive Neisseria meningitidis. Infect. Immun. 74, 5085–5094 (2006). This and subsequent studies by the authors examine Opa structural diversity in extensive collections of carriage and disease isolates of N. meningitidis.
Diaz Romero, J. & Outschoorn, I. M. Current status of meningococcal group B vaccine candidates: capsular or noncapsular? Clin. Microbiol. Rev. 7, 559–575 (1994).
Hammerschmidt, S. et al. Capsule phase variation in Neisseria meningitidis serogroup B by slipped-strand mispairing in the polysialyltransferase gene (siaD): correlation with bacterial invasion and the outbreak of meningococcal disease. Mol. Microbiol. 20, 1211–1220 (1996).
Deghmane, A. E., Giorgini, D., Larribe, M., Alonso, J. M. & Taha, M. K. Down-regulation of pili and capsule of Neisseria meningitidis upon contact with epithelial cells is mediated by CrgA regulatory protein. Mol. Microbiol. 43, 1555–1564 (2002).
Uria, M. J. et al. A generic mechanism in Neisseria meningitidis for enhanced resistance against bactericidal antibodies. J. Exp. Med. 205, 1423–1434 (2008).
Schneider, M. C., Exley, R. M., Ram, S., Sim, R. B. & Tang, C. M. Interactions between Neisseria meningitidis and the complement system. Trends Microbiol. 15, 233–240 (2007).
van Putten, J. P. & Robertson, B. D. Molecular mechanisms and implications for infection of lipopolysaccharide variation in Neisseria. Mol. Microbiol. 16, 847–853 (1995).
Parsons, N. J. et al. Cytidine 5′-monophospho-N-acetyl neuraminic acid and a low molecular weight factor from human blood cells induce lipopolysaccharide alteration in gonococci when conferring resistance to killing by human serum. Microb. Pathog. 5, 303–309 (1988).
Jones, C., Virji, M. & Crocker, P. R. Recognition of sialylated meningococcal lipopolysaccharide by siglecs expressed on myeloid cells leads to enhanced bacterial uptake. Mol. Microbiol 49, 1213–1225 (2003).
Brandtzaeg, P. et al. Neisseria meningitidis lipopolysaccharides in human pathology. J. Endotoxin Res. 7, 401–420 (2001).
Dunn, K. L., Virji, M. & Moxon, E. R. Investigations into the molecular basis of meningococcal toxicity for human endothelial and epithelial cells: the synergistic effect of LPS and pili. Microb. Pathog. 18, 81–96 (1995).
Lambotin, M. et al. Invasion of endothelial cells by Neisseria meningitidis requires cortactin recruitment by a phosphoinositide-3-kinase/Rac1 signalling pathway triggered by the lipo-oligosaccharide. J. Cell Sci. 118, 3805–3816 (2005).
Virji, M. et al. Opc- and pilus-dependent interactions of meningococci with human endothelial cells: molecular mechanisms and modulation by surface polysaccharides. Mol. Microbiol. 18, 741–754 (1995). Illustrates the interplay between several surface components of meningococci and its influence on host-cell interactions. Describes the mechanism of Opc-mediated invasion of endothelial cells.
Carbonnelle, E., Helaine, S., Nassif, X. & Pelicic, V. A systematic genetic analysis in Neisseria meningitidis defines the Pil proteins required for assembly, functionality, stabilization and export of type IV pili. Mol. Microbiol. 61, 1510–1522 (2006). In depth study on the molecular components that are involved in pilus assembly, functional maturation, emergence at the surface and retraction.
Virji, M. et al. Pilus-facilitated adherence of Neisseria meningitidis to human epithelial and endothelial cells: modulation of adherence phenotype occurs concurrently with changes in primary amino acid sequence and the glycosylation status of pilin. Mol. Microbiol. 10, 1013–1028 (1993).
Parge, H. E. et al. Structure of the fibre-forming protein pilin at 2.6 Å resolution. Nature 378, 32–38 (1995).
Virji, M. Post-translational modifications of meningococcal pili. Identification of common substituents: glycans and α-glycerophosphate — a review. Gene 192, 141–147 (1997). Review of the discovery of glycans and of the identification of digalactosyl 2,4-diacetamido-2,4, 6-trideoxyhexose on meningococcal pili. See reference 51 for a variant structure and reference 49 for genes involved in pilin glycosylation and frequency of variation.
Nassif, X. et al. Type-4 pili and meningococcal adhesiveness. Gene 192, 149–153 (1997).
Marceau, M. & Nassif, X. Role of glycosylation at Ser63 in production of soluble pilin in pathogenic Neisseria. J. Bacteriol. 181, 656–661 (1999).
Power, P. M. et al. Genetic characterization of pilin glycosylation and phase variation in Neisseria meningitidis. Mol. Microbiol. 49, 833–847 (2003).
Banerjee, A. & Ghosh, S. K. The role of pilin glycan in neisserial pathogenesis. Mol. Cell. Biochem. 253, 179–190 (2003).
Chamot-Rooke, J. et al. Alternative Neisseria spp. type IV pilin glycosylation with a glyceramido acetamido trideoxyhexose residue. Proc. Natl Acad. Sci. USA 104, 14783–14788 (2007).
Hegge, F. T. et al. Unique modifications with phosphocholine and phosphoethanolamine define alternate antigenic forms of Neisseria gonorrhoeae type IV pili. Proc. Natl Acad. Sci. USA 101, 10798–10803 (2004).
Weiser, J. N., Goldberg, J. B., Pan, N., Wilson, L. & Virji, M. The phosphorylcholine epitope undergoes phase variation on a 43-kilodalton protein in Pseudomonas aeruginosa and on pili of Neisseria meningitidis and Neisseria gonorrhoeae. Infect. Immun. 66, 4263–4267 (1998).
Lysenko, E. S., Gould, J., Bals, R., Wilson, J. M. & Weiser, J. N. Bacterial phosphorylcholine decreases susceptibility to the antimicrobial peptide LL-37/hCAP18 expressed in the upper respiratory tract. Infect. Immun. 68, 1664–1671 (2000).
Serino, L. & Virji, M. Genetic and functional analysis of the phosphorylcholine moiety of commensal Neisseria lipopolysaccharide. Mol. Microbiol. 43, 437–448 (2002).
Weiser, J. N., Shchepetov, M. & Chong, S. T. Decoration of lipopolysaccharide with phosphorylcholine: a phase-variable characteristic of Haemophilus influenzae. Infect. Immun. 65, 943–950 (1997).
Naessan, C. L. et al. Genetic and functional analyses of PptA, a phospho-form transferase targeting type IV pili in Neisseria gonorrhoeae. J. Bacteriol. 190, 387–400 (2008).
Warren, M. J. & Jennings, M. P. Identification and characterization of pptA: a gene involved in the phase-variable expression of phosphorylcholine on pili of Neisseria meningitidis. Infect. Immun. 71, 6892–6898 (2003).
Craig, L. et al. Type IV pilus structure by cryo–electron microscopy and crystallography: implications for pilus assembly and functions. Mol. Cell 23, 651–662 (2006).
Boslego, J. W. et al. Efficacy trial of a parenteral gonococcal pilus vaccine in men. Vaccine 9, 154–162 (1991).
Helaine, S., Dyer, D. H., Nassif, X., Pelicic, V. & Forest, K. T. 3D structure/function analysis of PilX reveals how minor pilins can modulate the virulence properties of type IV pili. Proc. Natl Acad. Sci. USA 104, 15888–15893 (2007).
Fussenegger, M., Rudel, T., Barten, R., Ryll, R. & Meyer, T. F. Transformation competence and type-4 pilus biogenesis in Neisseria gonorrhoeae — a review. Gene 192, 125–134 (1997).
Winther-Larsen, H. C. et al. A conserved set of pilin-like molecules controls type IV pilus dynamics and organelle-associated functions in Neisseria gonorrhoeae. Mol. Microbiol. 56, 903–917 (2005).
Merz, A. J., So, M. & Sheetz, M. P. Pilus retraction powers bacterial twitching motility. Nature 407, 98–102 (2000).
Maier, B. et al. Single pilus motor forces exceed 100 pN. Proc. Natl Acad. Sci. USA 99, 16012–16017 (2002). References 64 and 65 demonstrate that N. gonorrhoeae pili retract with a force that can exceed 80 picoNewton. The studies provide the evidence that single retraction events are powered by the action of a single PilT complex on a single pilus fibre.
Swanson, J. Studies on gonococcus infection. XIV. Cell wall protein differences among color/opacity colony variants of Neisseria gonorrhoeae. Infect. Immun. 21, 292–302 (1978).
Virji, M. et al. Expression of the Opc protein correlates with invasion of epithelial and endothelial cells by Neisseria meningitidis. Mol. Microbiol. 6, 2785–2795 (1992).
Hobbs, M. M. et al. Recombinational reassortment among opa genes from ET-37 complex Neisseria meningitidis isolates of diverse geographical origins. Microbiology 144, 157–166 (1998).
Milagres, L. G., Gorla, M. C., Sacchi, C. T. & Rodrigues, M. M. Specificity of bactericidal antibody response to serogroup B meningococcal strains in Brazilian children after immunization with an outer membrane vaccine. Infect. Immun. 66, 4755–4761 (1998).
de Jonge, M. I. et al. Functional activity of antibodies against the recombinant OpaJ protein from Neisseria meningitidis. Infect. Immun. 71, 2331–2340 (2003).
Seiler, A., Reinhardt, R., Sarkari, J., Caugant, D. A. & Achtman, M. Allelic polymorphism and site-specific recombination in the opc locus of Neisseria meningitidis. Mol. Microbiol. 19, 841–856 (1996).
Unkmeir, A. et al. Fibronectin mediates Opc-dependent internalization of Neisseria meningitidis in human brain microvascular endothelial cells. Mol. Microbiol. 46, 933–946 (2002).
Whalen, C. M., Hockin, J. C., Ryan, A. & Ashton, F. The changing epidemiology of invasive meningococcal disease in Canada, 1985 through 1992. Emergence of a virulent clone of Neisseria meningitidis. JAMA 273, 390–394 (1995).
Kriz, P., Vlckova, J. & Bobak, M. Targeted vaccination with meningococcal polysaccharide vaccine in one district of the Czech Republic. Epidemiol. Infect. 115, 411–418 (1995).
Peak, I. R., Srikhanta, Y., Dieckelmann, M., Moxon, E. R. & Jennings, M. P. Identification and characterisation of a novel conserved outer membrane protein from Neisseria meningitidis. FEMS Immunol. Med. Microbiol. 28, 329–334 (2000).
Scarselli, M. et al. Neisseria meningitidis NhhA is a multifunctional trimeric autotransporter adhesin. Mol. Microbiol. 61, 631–644 (2006).
Serruto, D. et al. Neisseria meningitidis App, a new adhesin with autocatalytic serine protease activity. Mol. Microbiol. 48, 323–334 (2003).
Schmitt, C. et al. A functional two-partner secretion system contributes to adhesion of Neisseria meningitidis to epithelial cells. J. Bacteriol. 189, 7968–7976 (2007).
Comanducci, M. et al. NadA, a novel vaccine candidate of Neisseria meningitidis. J. Exp. Med. 195, 1445–1454 (2002).
Comanducci, M. et al. NadA diversity and carriage in Neisseria meningitidis. Infect. Immun. 72, 4217–4223 (2004).
Ciabattini, A. et al. Intranasal immunization of mice with recombinant Streptococcus gordonii expressing NadA of Neisseria meningitidis induces systemic bactericidal antibodies and local IgA. Vaccine 26, 4244–4250 (2008).
Turner, D. P. et al. Characterization of MspA, an immunogenic autotransporter protein that mediates adhesion to epithelial and endothelial cells in Neisseria meningitidis. Infect. Immun. 74, 2957–2964 (2006).
Virji, M., Makepeace, K., Ferguson, D. J. P., Achtman, M. & Moxon, E. R. Meningococcal Opa and Opc proteins — their role in colonization and invasion of human epithelial and endothelial-cells. Mol. Microbiol. 10, 499–510 (1993).
Heckels, J. E., Blackett, B., Everson, J. S. & Ward, M. E. The influence of surface charge on the attachment of Neisseria gonorrhoeae to human cells. J. Gen. Microbiol. 96, 359–364 (1976).
Pinner, R. W., Spellman, P. A. & Stephens, D. S. Evidence for functionally distinct pili expressed by Neisseria meningitidis. Infect. Immun. 59, 3169–3175 (1991).
Jonsson, A. B., Ilver, D., Falk, P., Pepose, J. & Normark, S. Sequence changes in the pilus subunit lead to tropism variation of Neisseria gonorrhoeae to human tissue. Mol. Microbiol. 13, 403–416 (1994).
Kirchner, M. & Meyer, T. F. The PilC adhesin of the Neisseria type IV pilus-binding specificities and new insights into the nature of the host cell receptor. Mol. Microbiol. 56, 945–957 (2005).
Hardy, S. J., Christodoulides, M., Weller, R. O. & Heckels, J. E. Interactions of Neisseria meningitidis with cells of the human meninges. Mol. Microbiol. 36, 817–829 (2000).
Scheuerpflug, I., Rudel, T., Ryll, R., Pandit, J. & Meyer, T. F. Roles of PilC and PilE proteins in pilus-mediated adherence of Neisseria gonorrhoeae and Neisseria meningitidis to human erythrocytes and endothelial and epithelial cells. Infect. Immun. 67, 834–843 (1999).
Kallstrom, H. et al. Attachment of Neisseria gonorrhoeae to the cellular pilus receptor CD46: identification of domains important for bacterial adherence. Cell. Microbiol. 3, 133–143 (2001).
Tobiason, D. M. & Seifert, H. S. Inverse relationship between pilus-mediated gonococcal adherence and surface expression of the pilus receptor, CD46. Microbiology 147, 2333–2340 (2001).
Blom, A. M. et al. A novel interaction between type IV pili of Neisseria gonorrhoeae and the human complement regulator C4B-binding protein. J. Immunol. 166, 6764–6770 (2001).
Chen, T., Belland, R. J., Wilson, J. & Swanson, J. Adherence of pilus- Opa+ gonococci to epithelial cells in vitro involves heparan sulfate. J. Exp. Med. 182, 511–517 (1995).
Moore, J. et al. Recognition of saccharides by the OpcA, OpaD, and OpaB outer membrane proteins from Neisseria meningitidis. J. Biol. Chem. 280, 31489–31497 (2005).
van Putten, J. P. & Paul, S. M. Binding of syndecan-like cell surface proteoglycan receptors is required for Neisseria gonorrhoeae entry into human mucosal cells. EMBO J. 14, 2144–2154 (1995).
Virji, M. et al. Critical determinants of host receptor targeting by Neisseria meningitidis and Neisseria gonorrhoeae: identification of Opa adhesiotopes on the N-domain of CD66 molecules. Mol. Microbiol. 34, 538–551 (1999).
de Vries, F. P., Cole, R., Dankert, J., Frosch, M. & van Putten, J. P. M. Neisseria meningitidis producing the Opc adhesin binds epithelial cell proteoglycan receptors. Mol. Microbiol. 27, 1203–1212 (1998).
Prince, S. M., Achtman, M. & Derrick, J. P. Crystal structure of the OpcA integral membrane adhesin from Neisseria meningitidis. Proc. Natl Acad. Sci. USA 99, 3417–3421 (2002).
Virji, M., Makepeace, K. & Moxon, E. R. Distinct mechanisms of interactions of Opc-expressing meningococci at apical and basolateral surfaces of human endothelial-cells — the role of integrins in apical interactions. Mol. Microbiol. 14, 173–184 (1994).
Duensing, T. D. & Putten, J. P. Vitronectin binds to the gonococcal adhesin OpaA through a glycosaminoglycan molecular bridge. Biochem. J. 334 133–139 (1998).
Hammarstrom, S. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67–81 (1999).
Muenzner, P. et al. Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells. Infect. Immun. 68, 3601–3607 (2000).
Chen, T. & Gotschlich, E. C. CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins. Proc. Natl Acad. Sci. USA 93, 14851–14856 (1996).
van Kempen, M. J. P., Rijkers, G. T. & Van Cauwenberge, P. B. The immune response in adenoids and tonsils. Int. Arch. Allergy Immunol. 122, 8–19 (2000).
Emonts, M., Hazelzet, J. A., de Groot, R. & Hermans, P. W. Host genetic determinants of Neisseria meningitidis infections. Lancet Infect. Dis. 3, 565–577 (2003).
Hament, J. M., Kimpen, J. L., Fleer, A. & Wolfs, T. F. Respiratory viral infection predisposing for bacterial disease: a concise review. FEMS Immunol. Med. Microbiol. 26, 189–195 (1999).
Fahlgren, A. et al. Interferon-γ tempers the expression of carcinoembryonic antigen family molecules in human colon cells: a possible role in innate mucosal defence. Scand. J. Immunol. 58, 628–641 (2003).
Hammarstrom, S. & Baranov, V. Is there a role for CEA in innate immunity in the colon? Trends Microbiol. 9, 119–125 (2001).
Hill, D. J. & Virji, M. A novel cell-binding mechanism of Moraxella catarrhalis ubiquitous surface protein UspA: specific targeting of the N-domain of carcinoembryonic antigen-related cell adhesion molecules by UspA1. Mol. Microbiol. 48, 117–129 (2003).
Hill, D. J. et al. The variable P5 proteins of typeable and non-typeable Haemophilus influenzae target human CEACAM1. Mol. Microbiol. 39, 850–862 (2001).
Hill, D. J., Edwards, A. M., Rowe, H. A. & Virji, M. Carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-binding recombinant polypeptide confers protection against infection by respiratory and urogenital pathogens. Mol. Microbiol. 55, 1515–1527 (2005). Together with reference 30, this study provides a case for the protective role of certain receptor-blocking strategies to prevent infection by mucosal pathogens.
Conners, R. et al. The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil. EMBO J. 27, 1779–1789 (2008).
Simoes, M. J., Cunha, M., Almeida, F., Furtado, C. & Brum, L. Molecular surveillance of Neisseria meningitidis capsular switching in Portugal, 2002–2006. Epidemiol. Infect. 137, 161–165 (2008).
Ramsay, M. E., Andrews, N. J., Trotter, C. L., Kaczmarski, E. B. & Miller, E. Herd immunity from meningococcal serogroup C conjugate vaccination in England: database analysis. BMJ 326, 365–366 (2003).
Sharon, N. Carbohydrates as future anti-adhesion drugs for infectious diseases. Biochim. Biophys. Acta 1760, 527–537 (2006).
van Ulsen, P. & Tommassen, J. Protein secretion and secreted proteins in pathogenic Neisseriaceae. FEMS Microbiol. Rev. 30, 292–319 (2006).
Massari, P., King, C. A., Ho, A. Y. & Wetzler, L. M. Neisserial PorB is translocated to the mitochondria of HeLa cells infected with Neisseria meningitidis and protects cells from apoptosis. Cell. Microbiol. 5, 99–109 (2003).
van Putten, J. P. M., Duensing, T. D. & Carlson, J. Gonococcal invasion of epithelial cells driven by P.IA, a bacterial ion channel with GTP binding properties. J. Exp. Med. 188, 941–952 (1998).
Ayala, P. et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca2+ transients in infected epithelial cells. Cell. Microbiol. 7, 1736–1748 (2005).
Joiner, K. A., Scales, R., Warren, K. A., Frank, M. M. & Rice, P. A. Mechanism of action of blocking immunoglobulin G for Neisseria gonorrhoeae. J. Clin. Invest. 76, 1765–1772 (1985).
Rosenqvist, E. et al. Functional activities and epitope specificity of human and murine antibodies against the class 4 outer membrane protein (Rmp) of Neisseria meningitidis 1. Infect. Immun. 67, 1267–1276 (1999).
Lee, H. S., Ostrowski, M. A. & Gray-Owen, S. D. CEACAM1 dynamics during Neisseria gonorrhoeae suppression of CD4+ T lymphocyte activation. J. Immunol. 180, 6827–6835 (2008).
Madico, G. et al. Factor H binding and function in sialylated pathogenic Neisseriae is influenced by gonococcal, but not meningococcal, porin. J. Immunol. 178, 4489–4497 (2007).
Nassif, X. Interactions between encapsulated Neisseria meningitidis and host cells. Int. Microbiol. 2, 133–136 (1999).
Vandeputte-Rutten, L., Bos, M. P., Tommassen, J. & Gros, P. Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential. J. Biol. Chem. 278, 24825–24830 (2003).
Tala, A. et al. The HrpB–HrpA two-partner secretion system is essential for intracellular survival of Neisseria meningitidis. Cell. Microbiol. 10, 2461–2482 (2008).
Cunha, C. S., Griffiths, N. J., Murillo, I. & Virji, M. Neisseria meningitidis Opc invasin binds to the cytoskeletal protein alpha-actinin. Cell. Microbiol. 11, 389–405 (2009).
Acknowledgements
Research in my laboratory cited here has been funded by the Wellcome Trust, the Medical Research Council, the Meningitis Research Foundation, Meningitis UK and GlaxoSmithKline. I acknowledge my colleagues D. Hill and N. Griffiths for their dedicated participation and my collaborators S. Ram, A. Hadfield, R. Sessions, J. Derrick, L. Brady and D. Ferguson for unpublished data and images included in this Review. I also thank the anonymous referees for their comments.
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DATABASES
Entrez Genome Project
FURTHER INFORMATION
Sanger Institute Neisseria lactamica website
University of Oklahoma Neisseria gonorrhoeae genome Sequencing Strain FA website
Glossary
- Commensal bacterium
-
A bacterium that inhabits a host without apparent adverse effects to the host.
- Natural competence
-
An innate ability of bacteria to acquire DNA from the local environment and to assimilate genetic information through homologous recombination.
- Multilocus sequence typing
-
A technique used to characterize strains by their unique allelic profiles of a set of housekeeping genes.
- Phase variation
-
Reversible switching on and off of surface antigens that helps bacterial evasion of host immune responses.
- Anti-opsonic
-
A term applied to agents that prevent the binding of opsonins (for example, antibodies) that enhance phagocytosis.
- Serogroup
-
A designation denoting the immunochemistry (structure) of the capsule polysaccharides of Neisseria meningitidis; N. meningitidis strains are divided into serogroups based on the reactivity of strains with antibodies against distinct capsule structures.
- Meninges
-
Membranes that envelop the central nervous system.
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Virji, M. Pathogenic neisseriae: surface modulation, pathogenesis and infection control. Nat Rev Microbiol 7, 274–286 (2009). https://doi.org/10.1038/nrmicro2097
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DOI: https://doi.org/10.1038/nrmicro2097
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