Argonaute proteins constitute a highly diverse family of nucleic acid-guided proteins. They were first discovered in eukaryotes as key proteins in RNA interference systems, but homologous prokaryotic Argonaute proteins (pAgos) have also been found in archaea and bacteria. In this Progress article, we focus on long pAgo variants, a class of pAgos that are involved in nucleic acid-guided host defence against invading nucleic acids, and discuss the potential of pAgos in genome editing.
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Work in the authors' laboratory was financially supported by a grant from the Netherlands Organization of Scientific Research (NWO-ECHO grant 711013002 and NWO-TOP grant 714015001) to J.v.d.O., and by a long-term postdoctoral fellowship from the European Molecular Biology Organization (EMBO) to D.C.S. (ALTF 179–2015).
The authors declare no competing financial interests.
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Hegge, J., Swarts, D. & van der Oost, J. Prokaryotic Argonaute proteins: novel genome-editing tools?. Nat Rev Microbiol 16, 5–11 (2018). https://doi.org/10.1038/nrmicro.2017.73
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